CN1113678A

CN1113678A - Reproduction for cotton sterile strain and its matched breeding method

Info

Publication number: CN1113678A
Application number: CN 94106074
Authority: CN
Inventors: 宇文璞
Original assignee: Individual
Current assignee: Individual
Priority date: 1994-06-08
Filing date: 1994-06-08
Publication date: 1995-12-27
Anticipated expiration: 2014-06-08
Also published as: CN1040828C

Abstract

The sterile plant of bud yellow A recessive nuclear sterile strain selected by said method can be used as both mother plant and paternal plant concurrently, and the soil temp. in the depth of 5-15 cm below the ground surface is controlled in 26.9-36 deg.C from 3-5 days before effective flower blooming stage to end of the blooming stage, and after its boll-forming stage and ripening stage the cotton seeds are ingathered. under the condition of natural production the sterile plant rate of said seed is 100%, and its restoring source is extensive. Said method uses said seed as mother plant and uses the restorer line, such as sea-island cotton restorer line and terrestrial cotton restorer line as paternal plant to make interplanting and hybridization, so that its hybrid strain is strong in dominance and high in yield.

Description

Reproduction for cotton sterile strain and its matched breeding method

The present invention relates to plant hybridization production of hybrid seeds field, specifically numerous kind and a matched breeding method that grows cotton male sterile line.

Cotton crossbreed can significantly improve output of cotton, the method that at present prepares cotton crossbreed and adopted is, do maternal with hereditary recessive gms line, recover system and make male parent, hybridization preparation crossbreed, wherein, numerous kind of male sterile line is to adopt sterile strain and heterozygosis can educate strain to hybridize after pollinating, and gathers in the crops the seed of tying in the sterile strain.The male sterile line kind of this method breeding contains and respectively accounts for 50% educated strain and sterile strain, when its work female parent is the paternal hybrid production of hybrid seeds with recovery, need before flowering stage, manually to remove educated strain wherein, this just not only need add the plantation amount that is twice the male sterile line kind when the production of hybrid seeds, also need consume a large amount of artificial seedlings that remove, and be difficult to all can to educate strain and remove before flowering stage, so with the prepared crossbreed cost height of this numerous kind of producing method for seed, purity is low.For addressing this problem, it is the three series mating producing method for seed that the CN1015684B patent has proposed employing male sterile line, maintenance line, recovery, the breeding of this method male sterile line kind be with sterile be maternal, remain male parent and hybridize, gather in the crops its seed, be sterile plant rate and be 100% male sterile line kind, the weak point of this method is, the recovery system that the male sterile line kind of being bred is difficult to find, and when supporting preparation crossbreed, need special transformation to recover to be, so the production of hybrid seeds cycle is long, cost height, advantage are low.

Purpose of the present invention just provides that a kind of numerous kind of program is simple, and the seed advantage is strong, and sterile plant rate can reach the breeding method of 100% male sterile line kind, and a kind of cross-breeding method supporting with it is provided simultaneously.

The object of the present invention is achieved like this, utilize sterile strain changeable characteristics of its fertility under extraordinary temperature condition of the yellow A recessive gms line of bud, select the sterile strain double as female parent and the male parent of the yellow A recessive gms line of bud for use, before its effectively the flowers are in blossom florescence 3-5 days to effective florescence end, control the dark soil temperature of the following 5-15cm of ground surface at 26.9-36 ℃, treat that sterile strain blooms and produce fertile flower powder, selfing knot bell, ripe back its seed of results.Wherein said control soil temperature can not be crossed low or too high.Experiment shows that when the soil temperature that the following 5-15cm of ground surface is dark was lower than 26.9 ℃, the fertility of the sterile strain of the yellow A recessive gms line of bud can not change, and can cause a large amount of fruit abscission rates when being higher than 36 ℃, influences harvest.

Generally, the sterile strain of cotton male sterile line does not have fertility, and therefore sterile strain can not selfing be tied bell, breeding seed.Innovative point of the present invention is to make the sterile strain of the yellow A recessive gms line of bud to produce fertility under certain condition, through selfing knot bell, thus breeding seed, and the seed of being bred is under natural growthing condition, be still male sterile line, thereby can be hybridization, the preparation crossbreed with recovering.The male sterile line kind of the inventive method breeding is because be that sterile strain selfing produces, so the sterile plant rate of this seed is 100%.Therefore when the preparation crossbreed, needn't strengthen the plantation amount of male sterile line, manually remove the workload that to educate strain, reduce cost thereby also remove from.

The male sterile line kind of breeding of the present invention has kept recessive gms line to recover source advantage widely, and sea-island cotton, upland cotton and the hybrid thereof of using during the land for growing field crops produces all can do its recovery source, so be easy to filter out the advantage combination; Have normal corolla, column cap and nectary after this male sterile line kind growth, when the preparation crossbreed, the pollination mode is unrestricted.

The producing method for seed that matches with the yellow A recessive gms line of the bud of being bred kind provided by the invention is: the yellow A recessive gms line of the bud kind of selecting for use said method to breed is done maternal, recover system and make male parent, the seed of tying on maternal alternate plantation, hybridization, the results female parent with male parent.

The innovative point of this method is, the male sterile line of selecting for use has and recovers the source widely, does not need special transformation to recover system, has simplified the procedure of breeding, has reduced breeding cost, and prepared crossbreed has remarkable advantages.

Be described in further detail the present invention below in conjunction with embodiment.

The concrete grammar of breeding cotton male sterile line kind:

Select the sterile strain such as the sterile strain of chicken foot leaf A of the yellow A recessive gms line of bud for use, the sterile strain of 8748A, the low sterile strain of phenol A of dominance etc., before it is bloomed 3-5 days, to effective end of term, the plastic foil canopy is set, control the dark soil temperature of the following 5-15cm in the face of land at 26.9-36 ℃, produce the fertile flower powder through it, selfing knot bell, after the maturation, gather in the crops its seed, when continuing numerous kind afterwards, can select this seed for use, ridge ground routinely, sowing, effectively spend 3-5 days extremely effective florescence ends before flowering stage, frame film canopy, control ground temperature, consumed cost by reducing control ground temperature, can this seed be seen through the mulch film kind on the stalk of field prior to seeding with regard to covering with plastic film, see through mulch film behind the seed sprouting and grow in outside the mulch film, root growth is controlled in the desired scope at effective florescence ground temperature guaranteeing under mulch film.Its specific implementation method is:

The concrete mode of table 1 breeding male sterile lines

Select the sterile strain of the yellow A of bud for use	Soil depth	Soil temperature
			The sterile strain of 8748A	5	36℃
The sterile strain of chicken foot leaf A	10	30℃
			Dominance is hanged down the sterile strain of phenol A	15	26.9℃

The source of the yellow A recessive gms line of the selected bud of the present invention is: select for use 216 heterozygosis among the cotton sterile male line 81A of recessive nuclear gene control can educate strain, isolate sterile strain and No. 10 hybridization of middle cotton with true leaf 3-5 sheet color base mark, the filial generation after the hybridization is the yellow A recessive gms line of bud.

The economical character of the yellow A male sterile line of bud is good, is example with 8748A, can educate about strain plant height 85cm, and about sterile strain 75cm, the plant type compactness, precocity, the knot bell is strong, and single bell weighs 5.0 grams, ginning outturn 40%, 6060 meters/gram of fibre fineness, powerful 4.68 grams, 31.11 meters of breaking lengths, mature coefficient 1.68.

The source of the yellow A recessive gms line of bud 8748A: yellow A827A of bud and No. 12 hybridization of middle cotton transformation.

The source of the yellow A recessive gms line of bud chicken foot leaf A: yellow A8748A of bud and chicken foot leaf type recover system's hybridization transformation gained.

The source of the low phenol A of the yellow A recessive gms line of bud dominance: yellow A8748A of bud and dominance low-phenol cotton hybridization transformation gained.

The specific embodiment of the matched breeding of the cotton male sterile line of the present invention's breeding is:

The matched breeding example of the male sterile line of table 2 the present invention breeding

Select female parent for use	Select male parent for use	Recover the strain rate	Restoring degree
				The sterile strain of 8748A	Sea-island cotton recovers system new extra large number	100％	100％
Chicken foot leaf A	It is 2145 that upland cotton recovers	100％	100％
				8748A	It is 2145 that upland cotton recovers	100％	100％
Chicken foot leaf A	Sea-island cotton recovers system new extra large number	100％	100％

The male parent source that this method is selected for use is:

It is 2145 that upland cotton recovers: from the Ji cotton No. 17 preferred, its properties and characteristics is a mid-early maturity, bell weighs 5.3 grams, ginning outturn 43%, the long 29mm of suede, specific strength 16, micron value 4.2, anti-withering.

Sea-island cotton recovers new extra large No. 3 source of system, introduces from Xinjiang, belongs to the island cotton seed, zero formula fruit branch, the long 40mm of suede, 7000 meters/gram of fineness, brute force 4 grams, precocity.

Prepare crossbreed according to above method, can find out with the male sterile line recovery source of the inventive method breeding extensive, its recovery rate, restoring degree is 100%, prepared crossbreed is than assorted No. 11 volume increase 18.6 in the Handan of the supporting preparation of three line method, than middle cotton 12 volume increase 20%, fiber strength increases by 21.24%, and Cheng Ling many 15.6%.The long 37mm of land sea crossbreed suede, regularity 46%, than strong by 30.6, micron value 3.3, mid-early maturity.

Claims

1, a breeding method that grows cotton male sterile line, it is characterized in that selecting for use the sterile strain double as female parent and the male parent of the yellow A recessive gms line of bud, before its effectively the flowers are in blossom florescence 3-5 days to effective florescence end, control the dark soil temperature of the following 5-15cm of ground surface at 26.9-36 ℃, make its knot bell, ripe back its seed of results.

2, the breeding method of cotton male sterile line according to claim 1, it is characterized in that the yellow A recessive gms line of said bud be 8748A or chicken foot leaf A, the low phenol A of dominance one of them.

3,, it is characterized in that said control soil temperature is to adopt plastic mulching according to the breeding method of claim 1,2 described any one cotton male sterile lines.

4, a matched breeding method that grows cotton male sterile line, it is maternal to it is characterized in that selecting for use the male-sterile seed of breeding according to claim 1 to do, and recovers system and makes male parent, maternal look into male parent between plantation, hybridize, gather in the crops its maternal seed.

5, the matched breeding method of cotton male sterile line according to claim 4 is characterized in that said recovery is is that sea-island cotton recovers system.

6, the matched breeding method of cotton male sterile line according to claim 4 is characterized in that said recovery is is that upland cotton recovers system.

7, the matched breeding method of cotton male sterile line according to claim 5 is characterized in that it is No. 3, new sea that said sea-island cotton recovers system.

8, the matched breeding method of cotton male sterile line according to claim 6 is characterized in that it is 2145 that said upland cotton recovers system.