CN111303275A - 一种重组人生长激素及其制备方法与药物应用 - Google Patents
一种重组人生长激素及其制备方法与药物应用 Download PDFInfo
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- CN111303275A CN111303275A CN201911190141.3A CN201911190141A CN111303275A CN 111303275 A CN111303275 A CN 111303275A CN 201911190141 A CN201911190141 A CN 201911190141A CN 111303275 A CN111303275 A CN 111303275A
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Images
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
参数 | 制备例1 | 制备例2 | 制备例3 |
分批发酵培养基中的甘油(g/L) | 20 | 30 | 40 |
接种量(%) | 2.5 | 5 | 10 |
pH | 6.8 | 7.0 | 7.2 |
DO(%) | 40 | 30 | 45 |
L(+)阿拉伯糖(mM) | 0.5 | 1.0 | 0.2 |
发酵产量 | 250mg/L | 260mg/L | 270mg/L |
参数 | 制备例1 | 制备例4 | 制备例5 |
诱导时OD600 | 20 | 35 | 50 |
发酵结束时OD600 | 55 | 65 | 80 |
发酵周期(h) | 11.5 | 13 | 16.5 |
发酵产量 | 250mg/L | 300mg/L | 350mg/L |
Claims (8)
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Cited By (3)
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CN112226454A (zh) * | 2020-11-02 | 2021-01-15 | 杭州科迪赛生物科技有限公司 | 一种重组人生长激素的真核表达及其纯化方法 |
CN113845585A (zh) * | 2021-11-08 | 2021-12-28 | 浙江毓昌生物技术有限公司 | 使用金属螯合色谱法去除重组人生长激素内毒素的方法 |
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CN113845585A (zh) * | 2021-11-08 | 2021-12-28 | 浙江毓昌生物技术有限公司 | 使用金属螯合色谱法去除重组人生长激素内毒素的方法 |
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