CN111234036B - 非洲猪瘟病毒p72融合蛋白及其制备方法和应用 - Google Patents
非洲猪瘟病毒p72融合蛋白及其制备方法和应用 Download PDFInfo
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- CN111234036B CN111234036B CN202010163171.1A CN202010163171A CN111234036B CN 111234036 B CN111234036 B CN 111234036B CN 202010163171 A CN202010163171 A CN 202010163171A CN 111234036 B CN111234036 B CN 111234036B
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K39/00—Medicinal preparations containing antigens or antibodies
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/12011—Asfarviridae
- C12N2710/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C—CHEMISTRY; METALLURGY
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- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/12011—Asfarviridae
- C12N2710/12034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/01—DNA viruses
Abstract
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CN202010163171.1A CN111234036B (zh) | 2020-03-10 | 2020-03-10 | 非洲猪瘟病毒p72融合蛋白及其制备方法和应用 |
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CN202010163171.1A CN111234036B (zh) | 2020-03-10 | 2020-03-10 | 非洲猪瘟病毒p72融合蛋白及其制备方法和应用 |
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CN111234036A CN111234036A (zh) | 2020-06-05 |
CN111234036B true CN111234036B (zh) | 2022-10-28 |
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Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111549040A (zh) * | 2020-06-15 | 2020-08-18 | 畜科生物工程有限公司 | 表达非洲猪瘟病毒p72、B602L蛋白的重组腺病毒载体、重组腺病毒及构建方法 |
CN111662390A (zh) * | 2020-06-17 | 2020-09-15 | 天康生物(上海)有限公司 | 禽流感HA-Fd融合蛋白及其制备方法和疫苗 |
CN113940992B (zh) * | 2020-07-15 | 2023-01-17 | 浙江海隆生物科技有限公司 | 一种非洲猪瘟亚单位疫苗组合物及其制备方法和应用 |
CN112111005B (zh) * | 2020-09-09 | 2021-04-23 | 中国检验检疫科学研究院 | 一种能与阳性血清竞争结合非洲猪瘟病毒b646l抗原的单克隆抗体及其应用 |
CN113150079B (zh) * | 2021-01-21 | 2021-12-31 | 中国检验检疫科学研究院 | 一种真核表达的非洲猪瘟病毒p72抗原及其应用 |
CN114835822B (zh) * | 2022-04-22 | 2022-11-04 | 浙江洪晟生物科技股份有限公司 | 猪瘟病毒的多聚体疫苗及其制备方法 |
CN115197961B (zh) * | 2022-06-20 | 2024-04-16 | 岭南现代农业科学与技术广东省实验室肇庆分中心 | 一种非洲猪瘟病毒b602l重组蛋白稳定表达细胞系及其构建方法和应用 |
CN115044612A (zh) * | 2022-06-20 | 2022-09-13 | 岭南现代农业科学与技术广东省实验室肇庆分中心 | 一种非洲猪瘟病毒p72蛋白稳定表达细胞系及其构建方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110642926A (zh) * | 2019-12-02 | 2020-01-03 | 北京纳百生物科技有限公司 | 非洲猪瘟病毒p72重组蛋白、单克隆抗体及试纸 |
CN110760006A (zh) * | 2019-10-31 | 2020-02-07 | 河南省生物工程技术研究中心 | 一种非洲猪瘟免疫系统靶向基因工程疫苗 |
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Patent Citations (2)
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CN110760006A (zh) * | 2019-10-31 | 2020-02-07 | 河南省生物工程技术研究中心 | 一种非洲猪瘟免疫系统靶向基因工程疫苗 |
CN110642926A (zh) * | 2019-12-02 | 2020-01-03 | 北京纳百生物科技有限公司 | 非洲猪瘟病毒p72重组蛋白、单克隆抗体及试纸 |
Non-Patent Citations (2)
Title |
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Structure of the African swine fever virus major capsid protein p72;Liu, Qi等;《CELL RESEARCH》;20191130;第29卷(第11期);953-955 * |
非洲猪瘟病毒多表位融合蛋白P72的构建、表达及免疫学特性研究;郭晶等;《家畜生态学报》;20190630;第40卷(第6期);60-65 * |
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