CN111212658A - 双特异性抗体及其制备和使用方法 - Google Patents

双特异性抗体及其制备和使用方法 Download PDF

Info

Publication number
CN111212658A
CN111212658A CN201880059130.9A CN201880059130A CN111212658A CN 111212658 A CN111212658 A CN 111212658A CN 201880059130 A CN201880059130 A CN 201880059130A CN 111212658 A CN111212658 A CN 111212658A
Authority
CN
China
Prior art keywords
ser
gly
val
thr
leu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201880059130.9A
Other languages
English (en)
Other versions
CN111212658B (zh
Inventor
朱义
卡特里娜·贝科娃
比尔·布雷迪
布莱尔·伦肖
夏冬
高泽人
布莱恩·科瓦切维奇
乔纳森·K·法莱
菲尔·谭
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chengdu Bailidote Biological Pharmaceutical Co ltd
Seattle Immunization Co
Original Assignee
Sichuan Baili Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sichuan Baili Pharmaceutical Co Ltd filed Critical Sichuan Baili Pharmaceutical Co Ltd
Priority to CN202311257227.XA priority Critical patent/CN117343193A/zh
Publication of CN111212658A publication Critical patent/CN111212658A/zh
Application granted granted Critical
Publication of CN111212658B publication Critical patent/CN111212658B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2818—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD28 or CD152
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

Abstract

本公开提供了对人CTLA4、PD‑1或PD‑L1中的至少两种具有结合特异性的双特异性抗体。在一个实施方案中，双特异性抗体包括具有重链和轻链的IgG结构域，以及连接到重链的C端或轻链的N端的两个scFv部分，其中IgG结构域对第一抗原具有结合特异性，其中scFv部分对第二抗原具有结合特异性，并且其中第一抗原和第二抗原不同并且独立地选自α‑CTLA4、α‑PD‑1和α‑PD‑L1。

Description

双特异性抗体及其制备和使用方法
相关申请的交叉引用
本申请要求2017年11月2日提交的题为“双特异性抗体及其制备和使用方法(BISPECIFIC ANTIBODIES AND METHODS OF MAKING AND USING THEREOF)”的美国临时专利申请第62580845号的权益，其全部内容通过引用明确地并入本文。
技术领域
本公开总体涉及生物治疗技术领域，更具体地涉及制备和使用双特异性抗体。所有参考文献均全文引入本文。
背景技术
癌细胞发展出各种策略来逃避免疫监视。特异性肿瘤抗原的缺乏和主要组织相容性复合物(MHC)分子表达的丧失阻碍了T淋巴细胞对癌细胞的识别。免疫抑制性肿瘤微环境还有助于降低免疫系统对肿瘤细胞的识别。肿瘤微环境是由调节性T细胞、髓系来源的抑制细胞、肿瘤相关巨噬细胞、抑制性B细胞、肿瘤或基质细胞产生的免疫抑制细胞因子(如TGF-β或IL-10)以及调节T细胞功能的免疫检查点分子组成的免疫抑制细胞群来呈现的[Marshall HT等人，Front Oncol 2018，8：315]。
已经证明使用患者自身的免疫系统在保持正常组织完整的同时，在控制肿瘤生长和特异性消除肿瘤细胞方面是有效的。免疫疗法为治疗癌症提供了另一个角度[Khalil DN等人，ADV Cancer Res 2015，128：1-68]。
联合免疫系统的多种调节剂是免疫肿瘤学领域迅速发展的一个新领域。新的治疗药物可以通过多途径调节对肿瘤细胞的免疫应答，可以提高患者的应答率，在某些情况下降低毒性，从而极大地有益于癌症患者。
在癌症治疗中使用一种以上靶向免疫系统的单克隆抗体的联合治疗已显示比使用单一药物治疗更有效[Hellman MD等人，ADV Immunol 2016，130：251-77]。除了提高疗效和应答率外，联合治疗往往比单一药物治疗具有更大的毒性。调节免疫系统的双特异性药物对患者的毒性较小和/或更有效，与包括具有相同特异性的单克隆抗体的组合的治疗相比具有额外的作用机制。
发明内容
本公开涉及双特异性抗体，具体地，含有IgG组份的双特异性抗体因此克服了BiTE分子的快速清除，作为不需要患者免疫细胞离体扩增的现成疗法，具有优于CAR-T细胞疗法的优点。双特异性抗体的另一个优点是通过同时接合两个检查点受体来增强克服抑制性肿瘤微环境的能力。
本公开中的双特异性抗体可以与其它试剂例如T细胞接合器组合，并且进一步增强它们的活性。
本公开涉及双特异性抗体，其结合在免疫和肿瘤细胞上表达的两个不同靶标。两个靶标可以都是检查点抗原。在一个实施方案中，两个靶标可以都是免疫细胞上的检查点抗原。在一个实施方案中，两个靶标可以都是肿瘤细胞上的检查点抗原。在一个实施方案中，一个靶标是免疫细胞上的检查点抗原，另一个靶标是肿瘤细胞上的检查点抗原。在一个实施方案中，检查点抗原可以选自PD-1、PD-L1和CTLA4。在一个实施方案中，靶标可以包括PD-1、PD-L1和CTLA4的任何组合。
本公开还提供双特异性药物的组合物及其用于治疗癌症和自身免疫缺陷的治疗用途。
在一个实施方案中，本申请公开了双特异性抗体，其包括IgG重链和轻链，以及连接到重链的C端或轻链的N端的两个scFv部分(components)，其中IgG对第一抗原具有结合特异性，其中scFv部分对第二抗原具有结合特异性，并且其中第一抗原和第二抗原不同且独立地选自α-CTLA4、α-PD-1和α-PD-L1。
在一个实施方案中，双特异性抗体具有连接到重链C端的两个scFv部分。在一个实施方案中，第一抗原包括α-CTLA4，第二抗原包括α-PD-1或α-PD-L1。在另一个实施方案中，第一抗原包括α-PD-1或α-PD-L1，第二抗原包括α-CTLA4。
在一个实施方案中，双特异性抗体具有连接到轻链N端的两个scFv部分。在一个实施方案中，第一抗原包括α-PD-1或α-PD-L1，第二抗原包括α-CTLA4。在另一个实施方案中，第一抗原包含α-CTLA4，第二抗原包含α-PD-1或α-PD-L1。
在一个实施方案中，双特异性抗体是分离的单克隆抗体。
在一个实施方案中，双特异性抗体包括具有本文公开的序列的抗原肽序列。在一个实施方案中，双特异性抗体可以具有与公开的氨基酸序列具有至少70％、80％、90％、95％、98％或99％相似性的抗原肽序列。
在一个实施方案中，双特异性抗体包括具有本文公开的序列的抗原结合片段。在一个实施方案中，双特异性抗体可以具有与所公开的抗体序列具有至少70％、80％、90％、98％或99％相似性的序列的抗原结合片段。
在一个实施方案中，双特异性抗体可以具有对α-CTLA4、α-PD-1或α-PD-L1的结合亲和力，Kd不大于70nM、50nM、40nM、30nM、20nM、10nM或5nM。
在一个实施方案中，双特异性抗体可以具有对α-CTLA4和α-PD-1的结合亲和力，Kd不大于70nM、50nM、40nM、30nM、20nM、10nM或5nM。
在一个实施方案中，双特异性抗体可以具有对α-CTLA4和α-PD-L1的结合亲和力，Kd不大于70nM、50nM、40nM、30nM、20nM、10nM或5nM。
在一个实施方案中，双特异性抗体可以具有对α-CTLA4、α-PD-1或α-PD-L1中的两种的结合亲和力，Kd不大于70nM、50nM、40nM、30nM、20nM、10nM或5nM。
在一个实施方案中，双特异性抗体可以表现出一种或多种选自以下的功能特性：与α-CTLA4、α-PD-1或α-PD-L1的高亲和力结合，抑制PD-L1与PD-1的结合，增强T细胞活化，刺激抗体应答的能力和/或逆转免疫抑制细胞的抑制功能的能力，例如调节性T细胞。
在一个实施方案中，增强T细胞活化包括T细胞增殖、IFN-γ和/或IL-2分泌或其组合。
在一个实施方案中，双特异性抗体包括人源框架区域。
在一个实施方案中，双特异性抗体可以是人源化抗体、嵌合抗体或重组抗体。
在一个实施方案中，双特异性抗体包括IgG1恒定区以延长双特异性分子的循环半衰期。在一个实施方案中，双特异性抗体的IgG1恒定区包括与SEQ ID No.136具有至少98％相似性的氨基酸序列。
在一个实施方案中，本申请公开了分离的双特异性抗体，其选自描述的或具有本文公开的所述特征的那些克隆。
在一个实施方案中，本申请公开了双特异性抗体的IgG1重链，其包括选自本文公开的序列的氨基酸序列。在一个实施方案中，IgG1重链可具有与SEQ ID No.02、06、08、10、12、14、16、18、20、22、26、30、34、38、42、46、50、54、58、62、72、92、96、100、104、108、112、116、120、124、128或132具有至少70％、80％、90％、98％或99％相似性的氨基酸序列。
在一个实施方案中，本申请公开了双特异性抗体的κ轻链。在一个实施方案中，κ轻链可具有与SEQ ID No.04、28、32、36、40、44、48、52、56、60、和64具有至少70％、80％、90％、98％、或99％相似性的氨基酸序列。
在一个实施方案中，本申请公开了双特异性抗体的可变轻链，其包括本文公开的氨基酸序列。在一个实施方案中，可变轻链可以具有与SEQ ID No.94、98、102、106、110、114、118、122、126、130或134具有至少70％、80％、90％,98％或99％相似性的氨基酸序列。
在一个实施方案中，本申请公开了双特异性抗体的可变重链，其包括本文公开的氨基酸序列。在一个实施方案中，可变重链可具有与SEQ ID No.92、96、100、104、108、112、116、120、124、128或132具有至少70％、80％、90％、98％或99％相似性的氨基酸序列。
在一个实施方案中，本申请公开了编码双特异性抗体的分离的核酸，其包括本文公开的IgG1重链，本文公开的κ轻链，本文公开的可变轻链或本文公开的可变重链。在一个实施方案中，本申请公开了编码双特异性抗体的分离的核酸，其包括IgG1重链，所述IgG1重链具有与SEQ ID No.02、06、08、10、12、14、16、18、20、22、26、30、34、38、42、46、50、54、58、62、72、92、96、100、104,108、112、116、120、124、128或132具有至少70％、80％、90％、98％或99％相似性的氨基酸序列，κ轻链具有与SEQ ID No.04、28、32、36、40、44、48、52、56、60和64具有至少70％、80％、90％、98％或99％相似性的氨基酸序列，可变轻链具有与SEQ IDNo.94、98、102、106、110、114、118、122、126、130或134具有至少70％、80％、90％、98％或99％相似性的氨基酸序列，可变重链具有与SEQ ID No.92、96、100、104、108、112、116、120、124、128或132具有至少70％、80％、90％、98％或99％相似性的氨基酸序列。
在一个实施方案中，本申请公开了包括本文公开的分离的核酸的表达载体(vector)。在一个实施方案中，表达载体(vector)包括具有与本文公开的核酸序列具有至少70％、80％、90％、98％或99％相似性的序列的分离的核酸。
在一个实施方案中，表达载体(vector)可在细胞中表达。
在一个实施方案中，本申请公开了包括本文公开的核酸的宿主细胞。
在一个实施方案中，本申请公开了包括表达载体(vector)的宿主细胞。
在一些实施方案中，本申请公开了宿主细胞，其中宿主细胞是原核细胞或真核细胞。
在一些实施方案中，本申请公开了产生抗体的方法，包括培养其提供的宿主细胞以产生抗体。
在一些实施方案中，本申请公开了包括双特异性抗体和细胞毒类药物(cytotoxicagent)的免疫偶联物。在一些实施方案中，细胞毒类药物是化疗药物、生长抑制剂、毒素或放射性同位素。
在一个实施方案中，本申请公开了药物组合物，其包括双特异性抗体和药学上可接受的载体(carrier)。在一个实施方案中，本申请公开了药物组合物，其包括免疫偶联物和药学上可接受的载体(carrier)。在一个实施方案中，药物组合物还包括放射性同位素、放射性核素、毒素、治疗剂、化疗药物或其组合。
在一个实施方案中，本申请公开了治疗患有癌症的受试者的方法，包括向受试者施用有效量的本文公开的双特异性抗体。在一个实施方案中，癌症包括乳腺癌、结肠直肠癌、胰腺癌、头颈癌、黑素瘤、卵巢癌、前列腺癌、非小细胞肺癌、小细胞肺癌、神经胶质瘤、食管癌、鼻咽癌、肾癌、胃癌、肝癌、膀胱癌、宫颈癌、脑肿瘤、淋巴瘤、白血病、骨髓瘤。
在一个实施方案中，本申请公开了治疗患有癌症的受试者的方法，其中癌症包括表达PD-L1的细胞。
在一个实施方案中，本申请公开了治疗患有癌症的受试者的方法，进一步包括共同施用有效量的治疗剂。在一些实施方案中，治疗剂包括抗体、化疗药物、酶或其组合。
在一些实施方案中，治疗剂包括卡培他滨、顺铂、曲妥珠单抗、氟维司群、他莫昔芬、来曲唑、依西美坦、阿那曲唑、氨鲁米特、睾内酯、伏氯唑、福美坦、法曲唑、来曲唑、埃罗替尼、阿法替尼(lafatinib)、达沙替尼、吉非替尼、伊马替尼、帕唑替尼(pazopinib)、拉帕替尼、舒尼替尼、尼洛替尼、索拉非尼、nab-紫杉醇(nab-palitaxel)、其衍生物或组合。
在一个实施方案中，本申请公开了治疗患有癌症的受试者的方法，其中受试者是人。
在一个实施方案中，本申请公开了包括有效浓度的本文公开的双特异性抗体的溶液，其中溶液是受试者的血浆。
根据下面的详细描述，本申请的其他实施例对于本领域技术人员将变得显而易见，其中通过示出预期用于执行本申请的最佳模式来描述本申请的实施例。如将认识到的，本申请能够具有其它和不同的实施例，并且其若干细节能够在各种显而易见的方面进行修改，所有这些都不脱离本申请的精神和范围。因此，附图和详细描述将认为是说明性的，而不是限制性的。
附图说明
结合附图，通过下面的描述和所附权利要求，本公开的前述和其它特征将变得更加清楚。应当理解，这些附图仅描述了根据本公开安排的若干实施例，因此不应被认为是对其范围的限制，将通过使用附图以附加的特性和细节来描述本公开，其中：
图1示出了靶向CTLA4、PD-1和PD-L1抗原的示例性双特异性抗体的图；
图2示出了示例性抗PD-1抗体与CHO细胞系表面表达的PD-1抗原的结合；
图3描述了评估代表性双特异性抗体阻断CTLA4和CD80之间相互作用的能力的生化测定的结果；
图4示出了用超抗原SEB刺激PBMC；图4A示出了用PD224D1×CT4 IgG1空白和PL230C6×CT4 IgG1空白的处理；图4B示出了CT4×PD224D1 IgG1空白、CT4×PL221G5 IgG1和CT4×PL221G5 IgG1空白的处理；
图5示出了PD-1/PDL-1途径的信号测定的结果；
图6示出了树突细胞混合淋巴细胞反应研究的结果；
图7示出了调节性T细胞抑制测定的结果；图7A示出了CD8+T细胞的增殖；图7B示出了IFNg的产生；
图8示出了CD8 T细胞脱粒对用示例性双特异性抗体处理的应答；
图9示出了MiXeno HCC287小鼠肿瘤模型的结果；
图10示出了示例性双特异性抗体对流感特异性(Flu-specific)CD8+T细胞增殖的影响；
图11示出了PBMC对CEFT肽库的记忆应答的结果；以及
图12示出了示例性双特异性抗体增强重定向T细胞的细胞毒性。
具体实施方式
在以下对本申请的实施例的详细描述中，参考了附图，在附图中，相同的附图标记表示类似的元素，并且在附图中以示例的方式示出了可以实践本申请的具体实施例。充分详细地描述这些实施例以使得本领域的技术人员能够实践该应用。在其它情况下，未详细示出公知的回路、结构和技术，以免混淆对本说明书的理解。因此，下面的详细描述不是限制性的，本申请的范围仅由所附权利要求限定。
本公开涉及特异性结合人CTLA4、PD-1或PD-L1的双特异性抗体。在一些实施方案中，双特异性抗体包括结合CTLA4的第一臂和结合PD-1或PD-L1的第二臂。在一些实施方案中，双特异性抗体包括结合PD-1或PD-L1的第一臂和结合CTLA4的第二臂。包括臂的结构域的实例包括但不限于Fab和scFv结构域。每个臂含有两个抗原结合结构域并通过Fc结构域与另一个臂连接。Fc结构域可以是人IgG1、IgG2、IgG3、IgG4或工程化同种型。
本申请的双特异性抗体(图1)靶向人CTLA4、人PD-1和人PD-L1。这些靶向双特异性抗体各自携带抗CTLA4(SEQ Ids 91、93)和抗人PD-1(SEQ IDs 95、97、131、133)或PD-L1结合结构域(SEQ IDs 99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129)。任一结合结构域被转化为scFv(VH-VL方向)用于放置在C端，或scFv(VL-VH方向)用于放置在N端(分别为图1A和B)。
在一些实施方案中，本文所述的scFv分子含有20个氨基酸的柔性gly-gly-gly-gly-ser(G4S)X4接头，其可操作地连接VH和VL，而与V区方向(LH或HL)无关。双特异性抗体中的其余位置可由人IgG1 Fc或IgG1空白Fc重链、VH-CH1-铰链-CH2-CH3及其相应的κ轻链VL-CL组成。scFv结构域通过10-氨基酸(G4S)×2接头遗传连接到IgG1重链的N端或C端，产生连续的～100kDa重链单体肽。当与合适的轻链共转染时，可以通过人IgG1 Fc(蛋白A)纯化最终的对称双特异性分子并测定以评估功能活性。
预先构建重链和轻链基因“盒”，使得可以使用任一限制性酶切位点克隆V-区(图1A实例：重链的HindIII/NotI和轻链的HindIII/BsiWI)。在一个实施方案中，使用“无限制克隆”NEBui lder(NEB，Ipswich，MA)。
双特异性抗体通过包括设计完整分子、合成和克隆每个结构域的核苷酸序列、在哺乳动物细胞中表达和纯化终产物的方法产生。使用Geneous 10.2.3软件包(Biomaterials，奥克兰，新西兰)组装核苷酸序列，并将其分解成其用于基因合成的组成结构域(Genewiz，南普莱恩菲尔德(South Plainfield)，新泽西州)。在一个实施例中(图1A)，CT4×PD224D1 IgG1空白的双特异性抗体的重链(SEQ ID 2)由与人IgG1空白的Fc结构域(铰链，CH1和CH2)融合的抗CTLA4 VH结构域，随后与抗PD-1scFV结构域(克隆PD224D1)融合的10-氨基酸(G4S)x 2接头组成。使用基于NEBuilder Web的工具，将5'和3'核苷酸附加到每个结构域上，使得每个结构域与其侧翼结构域重叠20-30个核苷酸，这指导位点特异性重组，从而在单个基因组装步骤中遗传融合每个结构域。
CT4×PD224D1 IgG1空白的轻链由与人Cκ结构域融合的抗CTLA4 VL结构域组成。合成的基因片段用限制性酶HindIII和BsiWI酶切，然后与人Cκ结构域框内连接。对于这两种构建体，将小等分试样转化到大肠杆菌(E.coli)DH10b(Invitrogen，卡尔斯巴德(Carlsbad)，加利福尼亚州)中，并铺在TB+羧苄青霉素100μg/ml板(Teknova，霍利斯特(Hollister)，加利福尼亚州)上，在37℃孵育过夜。选择所得菌落并将2ml过夜培养物接种于TB+羧苄青霉素中。从过夜培养物中制备DNA(Thermo-Fisher，卡尔斯巴德(Carlsbad)，加利福尼亚州)，随后使用针对每个结构域侧翼序列的测序引物(Sigma，圣路易斯(St.Louis)，密苏里州(MO))对DNA进行测序(Genewiz，南普莱恩菲尔德(SouthPlainfield)，新泽西州)。所有DNA序列在Geneous中组装和分析。
实施例
实施例1：抗PD-L1抗体与PD-L1抗原的结合
在Fortebio Octet RED96仪器上通过表面等离子体共振(Surface PlasmonResonance)评价抗PD-L1抗体对PD-L1重组蛋白的结合亲和力和动力学。抗原固定在传感器芯片表面上，测试的抗体流过固定的抗原。所有分子显示出与抗原的强结合，例如如表1所示。
表1.抗PD-L1抗体与PD-L1抗原的结合
Figure BDA0002408401030000071
实施例2：抗PD-1抗体与PD-1抗原的结合
用流式细胞仪(FACS)法检测双特异性抗体及其部分与表达于CHO细胞表面的PD-1抗原的结合情况。将双特异性抗体与表达PD-1抗原的CHO细胞系孵育，然后用直接缀合Alexa Fluor 647荧光染料的第二抗人抗体检测。在流式细胞仪BD LSRFortessa上分析测试抗体的细胞结合。所有测试的抗体以个位数纳摩尔范围的KD与抗原结合(图2)。
实施例3：阻断CTLA4和CD80之间的相互作用
在生化相互作用测定(Cisbio)中测试双特异性抗体阻断CTLA4与其配体CD80之间相互作用的能力。简言之，将双特异性抗体与CTLA4和CD80蛋白孵育。然后向混合物中加入识别CTLA4和CD80蛋白并用HTRF供体/受体荧光对标记的检测抗体。CTLA4和CD80之间的相互作用通过FRET效率评估。所有测试的双特异性抗体都能够阻断CTLA4和CD80之间的相互作用(图3)。
实施例4：超抗原SEB刺激PBMC
评价双特异性抗体增强用超抗原SEB刺激后人外周血单核细胞(PBMC)释放细胞因子的能力。如图4A和图4B所示，所有双特异性分子在用SEB刺激后能够显著增强PBMC产生IL-2。
实施例5：PD-1/PD-L1通路信号传导
测试双特异性抗体阻断PD-1/PD-L1通路的能力。简言之，将测试分子与表达PD-1受体和荧光素酶NFAT报告基因的Jurkat报告基因细胞系和CHO-PD-L1细胞系(Promega)孵育。通过NFAT信号传导的增加评估测试抗体阻断通过PD-1/PD-L1通路的信号传导的能力。依次通过荧光素酶报告基因的活性监测NFAT信号传导。在平板读数器(Clariostar，BMG)上读取测定。所有测试的双特异性抗体和单克隆抗体对照都能够阻断PD-1/PD-L1信号传导(图5)。
实施例6：树突细胞混合白细胞反应
测试双特异性抗体增强树突细胞混合白细胞反应(MLR)的能力。将测试分子与来自一个供体的树突细胞和分离自另一个供体的T细胞孵育6天。树突细胞在GM-CSF和IL-4存在下从单核细胞体外分化。用干细胞分离试剂盒从PBMC中分离单核细胞和T细胞群。通过分泌的IFNγ评价测试分子增强MLR的能力。所有测试的双特异性抗体都能够增加IFNγ的产生，如图6所示。
实施例7：调节性T细胞对CD8 T细胞的抑制
测试双特异性抗体阻断调节性T细胞对效应CD8 T细胞增殖和细胞因子产生的抑制作用的能力。用干细胞分离试剂盒分离CD8 T细胞并用CellTrace染料(ThermoFisher)标记。如先前MLR研究中所述制备树突细胞。用干细胞分离试剂盒从PBMC中分离调节性T细胞并体外扩增。双特异性抗体与效应CD8 T细胞、树突细胞和调节性T细胞孵育4天。双特异性抗体在调节性T细胞存在下挽救效应CD8 T细胞功能的能力通过效应CD8 T细胞(图7A)和分泌的IFNγ的增殖来评估(图7B)。
实施例8：CD8 T细胞脱粒
在本研究中评估双特异性分子对细胞毒性CD8 T细胞具有作用的能力。简言之，用干细胞分离试剂盒纯化CD8 T细胞，并在双特异性测试分子存在下用CEFT肽库(JPTPeptide Technologies)刺激。培养基补充有IL-7和IL-21。在第11天，在存在布雷菲德菌素(Brefeldin)和莫能菌素(Monensin)以及直接用荧光染料标记的抗CD107a抗体的情况下用肽再刺激CD8 T细胞。24小时后，CD8 T细胞用直接缀合荧光染料的抗IFNγ抗体染色并在流式细胞仪BD LSRFortessa上检测。如图8所示，所有测试的双特异性抗体都能够增加细胞毒性IFNγ阳性T细胞的数量。
实施例9：Mixeno小鼠肿瘤模型
人源化小鼠模型用于评价这类双特异性抗体抑制体内肿瘤生长的能力。简言之，用人PBMC(每只小鼠5×106个细胞)重建NOG小鼠。在第3天，给动物皮下接种人肺癌细胞系HCC827(0.5E6细胞/动物)，开始用双特异性抗体和对照抗体进行两周一次的处理。每2至3天测量肿瘤体积。监测动物体重。测试的双特异性抗体能够比对照抗体更好地抑制肿瘤生长(图9)。
实施例10：流感特异性T细胞的增殖
在本研究中评估双特异性抗体增加抗原特异性CD8 T细胞增殖的能力。用干细胞分离试剂盒从PBMC中纯化CD8 T细胞，用流感特异性肽(JPT Peptide Technologies)脉冲，并在双特异性抗体存在下孵育14天。培养基补充有IL-7和IL-21。在第15天，细胞用肽特异性MHC dextramers(Immudex)染色，并在流式细胞仪BD LSRFortessa上评估。所有测试的双特异性抗体都能够增加抗原特异性CD8 T细胞的数量(图10)。
实施例11：PBMC对CEFT肽库的记忆应答
评估双特异性抗体增强T细胞记忆应答的能力。简言之，PBMC在CMV、EBV、流感和破伤风特异性肽(JPT Peptide Technologies)存在下孵育4-5天。定量检测分泌的IFNγ的量。如图11所示，相比于对照处理，双特异性CT4×PD224D1能够提高IFNγ的产量数倍。
实施例12：增强重定向T细胞细胞毒性
测试双特异性抗体增强针对肿瘤细胞系靶标的重定向T细胞细胞毒性(RTCC)的能力。肿瘤细胞系稳定表达通过慢病毒转导(Sartorius)递送的核定位红色荧光蛋白(RFP)。肿瘤细胞与PBMC和给定肿瘤细胞系特异性T细胞接合分子共培养。将双特异性抗体加入到共培养物中。通过计数RFP标记的肿瘤细胞核评价肿瘤细胞的裂解。在活细胞成像仪IncuCyte(Sartorius)上获得图像。孵育96小时后评价抗体的活性。在本研究中测试了4个PBMC供体。所有测试的双特异性抗体能够在至少一个测试的PBMC供体中增强RTCC活性(图12)。
虽然已参考特定实施例或实例描述了本发明，但应了解，所述实施例是说明性的且本发明的范围不限于此。本公开的替代实施例对于本公开所属领域的普通技术人员来说是显而易见的。这些替代实施例被认为包括在本公开的范围内。因此，本发明的范围由所附权利要求书限定且由上文描述支持。本公开中引用或提及的所有参考文献在此全文引入作为参考。
序列表
Figure BDA0002408401030000101
Figure BDA0002408401030000111
Figure BDA0002408401030000121
Figure BDA0002408401030000131
Figure BDA0002408401030000141
SEQ ID 01:CT4 x PD224D1核苷酸序列(nt)
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCA
CCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGAT
GGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCT
GCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACT
GGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAG
AGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTC
AGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGA
CCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAG
AGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGT
CTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGA
GCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAG
GAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAA
GTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCAC
AGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTAT
CCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTC
CGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCG
TGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGT
GGTGGATCCGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGC
CTCTGGATTCTCCCTCAGTAGCTATGCAATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTACATCGGCTACA
TTGGTGATACTACTGGCATAGCCTACGCGAGCTGGGCGAATGGCAGATTCACCATCTCCAAAGACAATACCAAGAACACG
GTGGATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGAGGCTGGTCCTACTTAGACAT
CTGGGGCCAAGGGACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCG
GTGGAGGCGGCTCTGCCCTTGTGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACT
TGCCAGGCCAGTCAGAACATTTACAGCAATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTA
TCAGGCCTCCACTCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATATGGGACAGATTTCACTCTCACCATCA
GCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAGGCGGTTATTATAGTGCTGCCCTTAATACTTTCGGCGGA
GGGACCAAGGTGGAGATCAAA
SEQ ID 02:CT4 x PD224D1氨基酸序列(aa)
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTASGFSLSSYAMSWVRQAPGKGLEYIGYIGDTTGIAYASWANGRFTISKDNTKNTVDLQMNSLRAEDTAVYYCARGWSYLDIWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSALVMTQSPSSLSASVGDRVTITCQASQNIYSNLAWYQQKPGKVPKLLIYQASTLASGVPSRFSGSGYGTDFTLTISSLQPEDVATYYCQGGYY SAALNTFGGGTKVEIK
SEQ ID 03:CT4κ轻链nt
GAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
CGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 04:CT4κ轻链aa
EIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 05:CT4 x PL230C6 nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGTCGGTGGAGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACCGCCTCTGGAATCGACCTTAATACCTACGACATGATCTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTTGGAATCATTACTTATAGTGGTAGTAGATACTACGCGAACTGGGCGAAAGGCCGATTCACCATCTCCAAAGACAATACCAAGAACACGGTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATTATATGAGTGGTTCCCACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCCGGTGGAGGCGGTTCAGGCGGAGGTGGAAGTGGTGGTGGCGGCTCTGGAGGCGGCGGATCTGCCTATGATATGACCCAGTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCAAGTGTCAGGCCAGTGAGGACATTTATAGCTTCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATTCTGCATCCTCTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTTATGGTAAAAATAATGTTGATAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 06:CT4 x PL230C6 aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQSVEESGGGLVQPGGSLRLSCTASGIDLNTYDMIWVRQAPGKGLEWVGIITYSGSRYYANWAKGRFTISKDNTKNTVYLQMNSLRAEDTAVYYCARDYMSGSHLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSAYDMTQSPSSVSASVGDRVTIKCQASEDIYSFLAWYQQKPGKAPKLLIHSASSLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGYG KNNVDNAFGGGTKVEIK
SEQ ID 07:CT4 x PL221G5空白2Fc nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCTTCAGTAGCGGGTACGACATGTGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGCATGCATTGCTGCTGGTAGTGCTGGTATCACTTACGACGCGAACTGGGCGAAAGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGATCGGCGTTTTCGTTCGACTACGCCATGGACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAGCATTAGTTCCCACTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTTACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGGGTTATAGTTGGGGTAATGTTGATAATGTTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 08:CT4 x PL221G5空白Fc aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSEVQLLESGGGLVQPGGSLRLSCAASGFSFSSGYDMCWVRQAPGKGLEWIACIAAGSAGITYDANWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARSAFSFDYAMDLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSTLSASVGDRVTITCQASQSISSHLNWYQQKPGKAPKLLIYKASTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQQGYSWGNVDNVFGGGTKVEIK
SEQ ID 09:CT4 x PL231H2 nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCACAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTCCAGCCTGGGGGCTCCCTGAGACTCTCCTGTACAACTTCTGGAATCGACCTTAGTACCTACGACATGATCTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGGAATCATTAGTTATGTTGGTAACACATACTACGCGAGCTGGGCGAAAGGCCGATTCACCCTCTCCAAAGACAATACCTCGACCACGGTGGATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATTTTATTAGTGGTTCCCACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGCCTATGATATGACCCAGTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGTCAGGCCAGTGAGAGCATTAGTAGCTTCTTATCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTTCTGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTTATAGTAAAAGTAATGTGGATAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 10:CT4 x PL231H2 aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGLVQPGGSLRLSCTTSGIDLSTYDMIWVRQAPGKGLEWVGIISYVGNTYYASWAKGRFTLSKDNTSTTVDLQMNSLRAEDTAVYYCARDFISGSHLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSAYDMTQSPSSVSASVGDRVTINCQASESISSFLSWYQQKPGKAPKLLIFSASTLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGY SKSNVDNAFGGGTKVEIK
SEQ ID 11:CT4 x PL004B5 nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCAGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCCTCAGTAGCTACTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGAGTCATTGATACTAATGTTTATATATACTACGCGAACTGGGCAAAAGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGATATGTGGGTAATAATGATGATTATATTAACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCAGAGTGTTTATAATGGCTACTGGTTATCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCTAGGCAGTTATACTAGTAGTACTGAGAACTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 12:CT4 x PL004B5 aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCAASGFSLSSYWMSWVRQAPGKGLEWIGVIDTNVYIYYANWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARYVGNNDDYINLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSTLSASVGDRVTITCQSSQSVYNGYWLSWYQQKPGKAPKLLIYGASTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCLGSYTSSTENSFGGGTKVEIK
SEQ ID 13:CT4 x PL004B9 nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCAGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGTGTCTGGAATCGACCTCAGTGTCATCAATATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGAACCATTACTTATGTTGGTAACACATATTACGCGAGCTGGGCGAAAGGCAGATTCACCATCTCCAAAACCTCGACCACGGTGGATCTTAAAATCACCAGTCCGACAACCGAGGACACGGCTGTGTATTACTGTGCGAGAGAATCTGGTACTATTTATTACAGTTACTTTAACTTGTGGGGCCAAGGGACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGCATTCGAATTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAAGTGCCAGGCCAGTGAAAGCATTAGCAACTACTTATCCTGGTATCAGCAGATTCCAGGGAAAGTTCCTAAGCTCCTGATCTATTATGCATCCAATCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAGCTATTATGGTGGTGGTAGTGCCTATACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 14:CT4 x PL004B9 aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTVSGIDLSVINMGWVRQAPGKGLEWIGTITYVGNTYYASWAKGRFTISKTSTTVDLKITSPTTEDTAVYYCARESGTIYYSYFNLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSAFELTQSPSSLSASVGDRVTIKCQASESISNYLSWYQQIPGKVPKLLIYYASNLASGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCQS YYGGGSAYTFGGGTKVEIK
SEQ ID 15:CT4 x PD206F12 nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCAGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCGACTTCAGTAGCGGCTACTGGATATGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTTGATCGCATGCATTTATGCTGGTACTAGTGGTAGTACTTCCTACGCGAGCTGGGCGAGAGGCAGATTCACCATCTCCGAAACCTCCAAGAACACGGTGACTCTTCAAATGAACAGCCTGAGAGCCGAGGACTCGGCTGTGTATTACTGTGCGAGAAATCTTTACACTTACAATAGCTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCAGAGTGTTTATGATAACAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATACAGTATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAGGCACTTATTATAGTAGTGGTTGGAACTTTGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 16:CT4 x PD206F12 aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCAASGFDFSSGYWICWVRQAPGKGLELIACIYAGTSGSTSYASWARGRFTISETSKNTVTLQMNSLRAEDSAVYYCARNLYTYNSLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSTLSASVGDRVTITCQSSQSVYDNNWLAWYQQKPGKAPKLLIYTVSTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQGTYYSSGWNFAFGGGTKVEIK
SEQ ID 17:CT4 x PD215A1 nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCACAGGAGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCTCCTTTAGCAGCTACTGGATGTGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGATGCATTACGACTGGTAGTGGTAGCACTTACTACGCGAGCTGGGCGAAGCGCCGGTTCACCATCTCCAAAGACAATTCCAAGAACACGGTGACTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTACGAGAGCATTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAGCATTTACAGCTACTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATCTGGCATCTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAAGCAGTTGGTTGAGTGGTGCTGTTGGTAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 18:CT4 x PD215A1 aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQEQLLESGGGLVQPGGSLRLSCTASGFSFSSYWMCWVRQAPGKGLEWIGCITTGSGSTYYASWAKRRFTISKDNSKNTVTLQMNSLRAEDTAVYYCTRAFDLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSSLSASVGDRVTITCQASQSIYSYLNWYQQKPGKAPKLLIYGASNLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQSSWLSG AVGNAFGGGTKVEIK
SEQ ID 19:CT4 x PD220F6 nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCACAGGAGCAGGTGAAGGAGACCGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATCAGCAGCTATGGAGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCGCATTGATTTTTCCCGGGATTGGTTTCAAAGACTACGCGAGCTGGGTGAATGGCCGGTTCACCCTCTCCAGCGACAACGCCCAGAACACTGTGGAACTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGAGATTTGGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCCGAGTGTTTATAGTAACTACTTATCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATTATGCATCCACTCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTGCAGGCGGTTATAGTAGTAGTACTCGTGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 20:CT4 x PD220F6 aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQEQVKETGGGLVQPGGSLRLSCAASGFTISSYGVSWVRQAPGKGLEWVALIFPGIGFKDYASWVNGRFTLSSDNAQNTVELQMNSLRAEDTAVYYCARDLDLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSSLSASVGDRVTITCQSSPSVYSNYLSWYQQKPGKVPKLLIYYASTLASGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCAGGYSS STRAFGGGTKVEIK
SEQ ID 21:CT4 x PD225G11 nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCAGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCAGCCACTGGATATGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGCATGCATTTATACTGGTAGTATTGATGTCTTTTACTACGCGAGCTGGGCGAAAGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGAGCCGCTAATACTGATACTACCTACTTTAACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGCCTATGATATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGCCAGGCCAGTCAGAGCATTAACAACCAACTATCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATGGTGCATCCACTCTGGCATCTGGGGTCCCATCTCGGTTCACCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCATGTTCATTATTGCAGTGGTGGTAGTTGTTTTTGGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 22:CT4 x PD225G11 aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSEVQLLESGGGLVQPGGSLRLSCAASGFTFSSSHWICWVRQAPGKGLEWIACIYTGSIDVFYYASWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARAANTDTTYFNLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSAYDMTQSPSSLSASVGDRVTINCQASQSINNQLSWYQQKPGKVPKLLIYGASTLASGVPSRFTGSGSGTDFTLTISSLQPEDVATYYCHVHYCSGGSCFWAFGGGTKVEIK
SEQ ID 23:G4S x 4 nt
GGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCT
SEQ ID 24:G4S x 4 aa
GGGGSGGGGSGGGGSGGGGS
SEQ ID 25:PL230C6 x CT4 nt
CAGTCGGTGGAGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACCGCCTCTGGAATCGACCTTAATACCTACGACATGATCTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTTGGAATCATTACTTATAGTGGTAGTAGATACTACGCGAACTGGGCGAAAGGCCGATTCACCATCTCCAAAGACAATACCAAGAACACGGTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATTATATGAGTGGTTCCCACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGTTCAGGCGGAGGTGGAAGTGGTGGTGGCGGCTCTGGAGGCGGCGGATCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 26:PL230C6 x CT4 aa
QSVEESGGGLVQPGGSLRLSCTASGIDLNTYDMIWVRQAPGKGLEWVGIITYSGSRYYANWAKGRFTISKDNTKNTVYLQMNSLRAEDTAVYYCARDYMSGSHLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYG SSPWTFGQGTKVEIK
SEQ ID 27:PL230C6κ轻链nt
GCCTATGATATGACCCAGTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCAAGTGTCAGGCCAGTGAGGACATTTATAGCTTCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATTCTGCATCCTCTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTTATGGTAAAAATAATGTTGATAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
CGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 28:PL230C6κ轻链aa
AYDMTQSPSSVSASVGDRVTIKCQASEDIYSFLAWYQQKPGKAPKLLIHSASSLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGYGKNNVDNAFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 29:PD224D1 x CT4 nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCTCCCTCAGTAGCTATGCAATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTACATCGGCTACATTGGTGATACTACTGGCATAGCCTACGCGAGCTGGGCGAATGGCAGATTCACCATCTCCAAAGACAATACCAAGAACACGGTGGATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGAGGCTGGTCCTACTTAGACATCTGGGGCCAAGGGACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 30:PD224D1 x CT4 aa
EVQLVESGGGLVQPGGSLRLSCTASGFSLSSYAMSWVRQAPGKGLEYIGYIGDTTGIAYASWANGRFTISKDNTKNTVDLQMNSLRAEDTAVYYCARGWSYLDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYG SSPWTFGQGTKVEIK
SEQ ID 31:PD224轻链nt
GCCCTTGTGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAACATTTACAGCAATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATCAGGCCTCCACTCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATATGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAGGCGGTTATTATAGTGCTGCCCTTAATACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 32:PD224轻链aa
ALVMTQSPSSLSASVGDRVTITCQASQNIYSNLAWYQQKPGKVPKLLIYQASTLASGVPSRFSGSGYGTDFTLTISSLQPEDVATYYCQGGYYSAALNTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 33:PL221G5 x CT4空白Fc nt
GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCTTCAGTAGCGGGTACGACATGTGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGCATGCATTGCTGCTGGTAGTGCTGGTATCACTTACGACGCGAACTGGGCGAAAGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGATCGGCGTTTTCGTTCGACTACGCCATGGACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 34:PL221G5 x CT4空白Fc aa
EVQLLESGGGLVQPGGSLRLSCAASGFSFSSGYDMCWVRQAPGKGLEWIACIAAGSAGITYDANWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARSAFSFDYAMDLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIK
SEQ ID 35:PL221G5轻链nt
GACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAGCATTAGTTCCCACTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTTACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGGGTTATAGTTGGGGTAATGTTGATAATGTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 36:PL221G5轻链aa
DIQMTQSPSTLSASVGDRVTITCQASQSISSHLNWYQQKPGKAPKLLIYKASTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQQGYSWGNVDNVFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 37:PL231H2 x CT4空白Fc nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTCCAGCCTGGGGGCTCCCTGAGACTCTCCTGTACAACTTCTGGAATCGACCTTAGTACCTACGACATGATCTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGGAATCATTAGTTATGTTGGTAACACATACTACGCGAGCTGGGCGAAAGGCCGATTCACCCTCTCCAAAGACAATACCTCGACCACGGTGGATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATTTTATTAGTGGTTCCCACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 38:PL231H2 x CT4空白Fc aa
QVQLVESGGGLVQPGGSLRLSCTTSGIDLSTYDMIWVRQAPGKGLEWVGIISYVGNTYYASWAKGRFTLSKDNTSTTVDLQMNSLRAEDTAVYYCARDFISGSHLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQY GSSPWTFGQGTKVEIK
SEQ ID 39:PL231H2轻链nt
GCCTATGATATGACCCAGTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGTCAGGCCAGTGAGAGCATTAGTAGCTTCTTATCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTTCTGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTTATAGTAAAAGTAATGTGGATAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 40:PL231H2轻链aa
AYDMTQSPSSVSASVGDRVTINCQASESISSFLSWYQQKPGKAPKLLIFSASTLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGYSKSNVDNAFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 41:PL004B5 x CT4 nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCCTCAGTAGCTACTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGAGTCATTGATACTAATGTTTATATATACTACGCGAACTGGGCAAAAGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGATATGTGGGTAATAATGATGATTATATTAACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 42:PL004B5 x CT4 aa
EVQLVESGGGLVQPGGSLRLSCAASGFSLSSYWMSWVRQAPGKGLEWIGVIDTNVYIYYANWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARYVGNNDDYINLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIK
SEQ ID 43:PL004B5轻链nt
GACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCAGAGTGTTTATAATGGCTACTGGTTATCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCTAGGCAGTTATACTAGTAGTACTGAGAACTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 44:PL004B5轻链aa
DIQMTQSPSTLSASVGDRVTITCQSSQSVYNGYWLSWYQQKPGKAPKLLIYGASTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCLGSYTSSTENSFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 45:PL004B9 x CT4 nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGTGTCTGGAATCGACCTCAGTGTCATCAATATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGAACCATTACTTATGTTGGTAACACATATTACGCGAGCTGGGCGAAAGGCAGATTCACCATCTCCAAAACCTCGACCACGGTGGATCTTAAAATCACCAGTCCGACAACCGAGGACACGGCTGTGTATTACTGTGCGAGAGAATCTGGTACTATTTATTACAGTTACTTTAACTTGTGGGGCCAAGGGACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 46:PL004B9 x CT4 aa
EVQLVESGGGLVQPGGSLRLSCTVSGIDLSVINMGWVRQAPGKGLEWIGTITYVGNTYYASWAKGRFTISKTSTTVDLKITSPTTEDTAVYYCARESGTIYYSYFNLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQ QYGSSPWTFGQGTKVEIK
SEQ ID 47:PL004B9轻链nt
GCATTCGAATTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAAGTGCCAGGCCAGTGAAAGCATTAGCAACTACTTATCCTGGTATCAGCAGATTCCAGGGAAAGTTCCTAAGCTCCTGATCTATTATGCATCCAATCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAGCTATTATGGTGGTGGTAGTGCCTATACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 48:PL004B9轻链aa
AFELTQSPSSLSASVGDRVTIKCQASESISNYLSWYQQIPGKVPKLLIYYASNLASGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCQSYYGGGSAYTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 49:PD206F12 x CT4 nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCGACTTCAGTAGCGGCTACTGGATATGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTTGATCGCATGCATTTATGCTGGTACTAGTGGTAGTACTTCCTACGCGAGCTGGGCGAGAGGCAGATTCACCATCTCCGAAACCTCCAAGAACACGGTGACTCTTCAAATGAACAGCCTGAGAGCCGAGGACTCGGCTGTGTATTACTGTGCGAGAAATCTTTACACTTACAATAGCTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 50:PD206F12 x CT4 aa
EVQLVESGGGLVQPGGSLRLSCAASGFDFSSGYWICWVRQAPGKGLELIACIYAGTSGSTSYASWARGRFTISETSKNTVTLQMNSLRAEDSAVYYCARNLYTYNSLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQ QYGSSPWTFGQGTKVEIK
SEQ ID 51:PD206F12轻链nt
GACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCAGAGTGTTTATGATAACAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATACAGTATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAGGCACTTATTATAGTAGTGGTTGGAACTTTGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 52:PD206F12轻链aa
DIQMTQSPSTLSASVGDRVTITCQSSQSVYDNNWLAWYQQKPGKAPKLLIYTVSTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQGTYYSSGWNFAFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 53:PD215A1 x CT4 nt
CAGGAGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCTCCTTTAGCAGCTACTGGATGTGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGATGCATTACGACTGGTAGTGGTAGCACTTACTACGCGAGCTGGGCGAAGCGCCGGTTCACCATCTCCAAAGACAATTCCAAGAACACGGTGACTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTACGAGAGCATTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 54:PD215A1 x CT4 aa
QEQLLESGGGLVQPGGSLRLSCTASGFSFSSYWMCWVRQAPGKGLEWIGCITTGSGSTYYASWAKRRFTISKDNSKNTVTLQMNSLRAEDTAVYYCTRAFDLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSS PWTFGQGTKVEIK
SEQ ID 55:PD215A1轻链nt
GACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAGCATTTACAGCTACTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATCTGGCATCTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAAGCAGTTGGTTGAGTGGTGCTGTTGGTAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ IF 56:PD215A1轻链aa
DIQMTQSPSSLSASVGDRVTITCQASQSIYSYLNWYQQKPGKAPKLLIYGASNLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQSSWLSGAVGNAFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 57:PD220F6 x CT4 nt
CAGGAGCAGGTGAAGGAGACCGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATCAGCAGCTATGGAGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCGCATTGATTTTTCCCGGGATTGGTTTCAAAGACTACGCGAGCTGGGTGAATGGCCGGTTCACCCTCTCCAGCGACAACGCCCAGAACACTGTGGAACTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGAGATTTGGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 58:PD220F6 x CT4 aa
QEQVKETGGGLVQPGGSLRLSCAASGFTISSYGVSWVRQAPGKGLEWVALIFPGIGFKDYASWVNGRFTLSSDNAQNTVELQMNSLRAEDTAVYYCARDLDLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSS PWTFGQGTKVEIK
SEQ ID 59:PD220F6轻链nt
GACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCCGAGTGTTTATAGTAACTACTTATCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATTATGCATCCACTCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTGCAGGCGGTTATAGTAGTAGTACTCGTGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 60:PD220F6轻链aa
DIQMTQSPSSLSASVGDRVTITCQSSPSVYSNYLSWYQQKPGKVPKLLIYYASTLASGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCAGGYSSSTRAFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 61:PD225G11 x CT4 nt
GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCAGCCACTGGATATGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGCATGCATTTATACTGGTAGTATTGATGTCTTTTACTACGCGAGCTGGGCGAAAGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGAGCCGCTAATACTGATACTACCTACTTTAACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTGGCGGTGGAGGGTCCGGCGGTGGTGGATCCCAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 62:PD225G11 x CT4 aa
EVQLLESGGGLVQPGGSLRLSCAASGFTFSSSHWICWVRQAPGKGLEWIACIYTGSIDVFYYASWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARAANTDTTYFNLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGGGGGSGGGGSQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIK
SEQ ID 63:PD225G11轻链nt
GCCTATGATATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGCCAGGCCAGTCAGAGCATTAACAACCAACTATCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATGGTGCATCCACTCTGGCATCTGGGGTCCCATCTCGGTTCACCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCATGTTCATTATTGCAGTGGTGGTAGTTGTTTTTGGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT
SEQ ID 64:PD225G11轻链aa
AYDMTQSPSSLSASVGDRVTINCQASQSINNQLSWYQQKPGKVPKLLIYGASTLASGVPSRFTGSGSGTDFTLTISSLQPEDVATYYCHVHYCSGGSCFWAFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
SEQ ID 65:CT4重链信号肽核苷酸序列nt
ATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTCCTGTCC
SEQ ID 66:CT4重链信号肽核苷酸序列aa
MKHLWFFLLLVAAPRWVLS
SEQ ID 67:PL230/PD224重链信号肽nt
ATGGCTGTCTTGGGGCTGCTCTTCTGCCTGGTGACATTCCCAAGCTGTGTCCTATCC
SEQ ID 68:PL230/PD224重链信号肽aa
MAVLGLLFCLVTFPSCVLS
SEQ ID 69:PL230/PD224轻链信号肽nt
ATGAGGGCCCCTGCTCAGTTTCTTGGCTTCTTGCTTTTCTGGATTCCAGCCTCCAGAAGT
SEQ ID 70:PL230/PD224轻链信号肽aa
MRAPAQFLGFLLFWIPASRS
SEQ ID 71:CT4 scFv nt
CAGGTGCAATTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGATGGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 72:CT4 scFv aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSEIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIK
SEQ ID73:PD224D1 scFv nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGC
CTCTGGATTCTCCCTCAGTAGCTATGCAATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTACATCGGCTACA
TTGGTGATACTACTGGCATAGCCTACGCGAGCTGGGCGAATGGCAGATTCACCATCTCCAAAGACAATACCAAGAACACG
GTGGATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGAGGCTGGTCCTACTTAGACAT
CTGGGGCCAAGGGACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCG
GTGGAGGCGGCTCTGCCCTTGTGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACT
TGCCAGGCCAGTCAGAACATTTACAGCAATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTA
TCAGGCCTCCACTCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATATGGGACAGATTTCACTCTCACCATCA
GCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAGGCGGTTATTATAGTGCTGCCCTTAATACTTTCGGCGGA
GGGACCAAGGTGGAGATCAAA
SEQ ID 74:PD224D1 scFv aa
EVQLVESGGGLVQPGGSLRLSCTASGFSLSSYAMSWVRQAPGKGLEYIGYIGDTTGIAYASWANGRFTISKDNTKNTVDLQMNS
LRAEDTAVYYCARGWSYLDIWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSALVMTQSPSSLSASVGDRVTITCQASQNIYSN
LAWYQQKPGKVPKLLIYQASTLASGVPSRFSGSGYGTDFTLTISSLQPEDVATYYCQGGYYSAALNTFGGGTKVEIK
SEQ ID 75:PD206F12 scFv nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCGACTTCAGTAGCGGCTACTGGATATGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTTGATCGCATGCATTTATGCTGGTACTAGTGGTAGTACTTCCTACGCGAGCTGGGCGAGAGGCAGATTCACCATCTCCGAAACCTCCAAGAACACGGTGACTCTTCAAATGAACAGCCTGAGAGCCGAGGACTCGGCTGTGTATTACTGTGCGAGAAATCTTTACACTTACAATAGCTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCAGAGTGTTTATGATAACAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATACAGTATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAGGCACTTATTATAGTAGTGGTTGGAACTTTGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 76:PD206F12 scFv aa
EVQLVESGGGLVQPGGSLRLSCAASGFDFSSGYWICWVRQAPGKGLELIACIYAGTSGSTSYASWARGRFTISETSKNTVTLQMNSLRAEDSAVYYCARNLYTYNSLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSTLSASVGDRVTITCQSSQSVYDNNWLAWYQQKPGKAPKLLIYTVSTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQGTYYSSGWNFAFGGGTKVEIK
SEQ ID 77:PD215A1 scFv nt
CAGGAGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCTCCTTTAGCAGCTACTGGATGTGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGATGCATTACGACTGGTAGTGGTAGCACTTACTACGCGAGCTGGGCGAAGCGCCGGTTCACCATCTCCAAAGACAATTCCAAGAACACGGTGACTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTACGAGAGCATTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAGCATTTACAGCTACTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATCTGGCATCTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAAGCAGTTGGTTGAGTGGTGCTGTTGGTAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 78:PD215A1 scFv aa
QEQLLESGGGLVQPGGSLRLSCTASGFSFSSYWMCWVRQAPGKGLEWIGCITTGSGSTYYASWAKRRFTISKDNSKNTVTLQMNSLRAEDTAVYYCTRAFDLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSSLSASVGDRVTITCQASQSIYSYLNWYQQKPGKAPKLLIYGASNLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQS SWLSGAVGNAFGGGTKVEIK
SEQ ID 79:PD225G11 scFv nt
GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCAGCCACTGGATATGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGCATGCATTTATACTGGTAGTATTGATGTCTTTTACTACGCGAGCTGGGCGAAAGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGAGCCGCTAATACTGATACTACCTACTTTAACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGCCTATGATATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGCCAGGCCAGTCAGAGCATTAACAACCAACTATCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATGGTGCATCCACTCTGGCATCTGGGGTCCCATCTCGGTTCACCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCATGTTCATTATTGCAGTGGTGGTAGTTGTTTTTGGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 80:PD225G11 scFv aa
EVQLLESGGGLVQPGGSLRLSCAASGFTFSSSHWICWVRQAPGKGLEWIACIYTGSIDVFYYASWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARAANTDTTYFNLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSAYDMTQSPSSLSASVGDRVTINCQASQSINNQLSWYQQKPGKVPKLLIYGASTLASGVPSRFTGSGSGTDFTLTISSLQPEDVATYYCHVHYCSGGSCFWAFGGGTKVEIK
SEQ ID 81:PL230C6 scFv nt
CAGTCGGTGGAGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACCGCCTCTGGAATCGACCTTAATACCTACGACATGATCTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTTGGAATCATTACTTATAGTGGTAGTAGATACTACGCGAACTGGGCGAAAGGCCGATTCACCATCTCCAAAGACAATACCAAGAACACGGTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATTATATGAGTGGTTCCCACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCCGGTGGAGGCGGTTCAGGCGGAGGTGGAAGTGGTGGTGGCGGCTCTGGAGGCGGCGGATCTGCCTATGATATGACCCAGTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCAAGTGTCAGGCCAGTGAGGACATTTATAGCTTCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATTCTGCATCCTCTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTTATGGTAAAAATAATGTTGATAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 82:PL230C6 scFv aa
QSVEESGGGLVQPGGSLRLSCTASGIDLNTYDMIWVRQAPGKGLEWVGIITYSGSRYYANWAKGRFTISKDNTKNTVYLQMNSLRAEDTAVYYCARDYMSGSHLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSAYDMTQSPSSVSASVGDRVTIKCQASEDIYSFLAWYQQKPGKAPKLLIHSASSLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGYGKNNVDNAFGGGTKVEIK
SEQ ID 83:PL231H2 scFv nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTCCAGCCTGGGGGCTCCCTGAGACTCTCCTGTACAACTTCTGGAATCGACCTTAGTACCTACGACATGATCTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGGAATCATTAGTTATGTTGGTAACACATACTACGCGAGCTGGGCGAAAGGCCGATTCACCCTCTCCAAAGACAATACCTCGACCACGGTGGATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATTTTATTAGTGGTTCCCACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGTTCTGCCTATGATATGACCCAGTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGTCAGGCCAGTGAGAGCATTAGTAGCTTCTTATCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTTCTGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTTATAGTAAAAGTAATGTGGATAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 84:PL231H2 scFv aa
QVQLVESGGGLVQPGGSLRLSCTTSGIDLSTYDMIWVRQAPGKGLEWVGIISYVGNTYYASWAKGRFTLSKDNTSTTVDLQMNSLRAEDTAVYYCARDFISGSHLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSAYDMTQSPSSVSASVGDRVTINCQASESISSFLSWYQQKPGKAPKLLIFSASTLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGYSKSNVDNAFGGGTKVEIK
SEQ ID 85:PL221G5 scFv nt
GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCTTCAGTAGCGGGTACGACATGTGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGCATGCATTGCTGCTGGTAGTGCTGGTATCACTTACGACGCGAACTGGGCGAAAGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGATCGGCGTTTTCGTTCGACTACGCCATGGACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAGCATTAGTTCCCACTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTTACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGGGTTATAGTTGGGGTAATGTTGATAATGTTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 86:PL221G5 scFv aa
EVQLLESGGGLVQPGGSLRLSCAASGFSFSSGYDMCWVRQAPGKGLEWIACIAAGSAGITYDANWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARSAFSFDYAMDLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSTLSASVGDRVTITCQASQSISSHLNWYQQKPGKAPKLLIYKASTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQQGYSWGNVDNVFGGGTKVEIK
SEQ ID 87:PL004B5 scFv nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCCTCAGTAGCTACTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGAGTCATTGATACTAATGTTTATATATACTACGCGAACTGGGCAAAAGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGATATGTGGGTAATAATGATGATTATATTAACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCAGAGTGTTTATAATGGCTACTGGTTATCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCTAGGCAGTTATACTAGTAGTACTGAGAACTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 88:PL004B5 scFv aa
EVQLVESGGGLVQPGGSLRLSCAASGFSLSSYWMSWVRQAPGKGLEWIGVIDTNVYIYYANWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARYVGNNDDYINLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSTLSASVGDRVTITCQSSQSVYNGYWLSWYQQKPGKAPKLLIYGASTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCLGSYTSSTENSFGGGTKVEIK
SEQ ID 89:PL004B9 scFv nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGTGTCTGGAATCGACCTCAGTGTCATCAATATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGAACCATTACTTATGTTGGTAACACATATTACGCGAGCTGGGCGAAAGGCAGATTCACCATCTCCAAAACCTCGACCACGGTGGATCTTAAAATCACCAGTCCGACAACCGAGGACACGGCTGTGTATTACTGTGCGAGAGAATCTGGTACTATTTATTACAGTTACTTTAACTTGTGGGGCCAAGGGACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGCATTCGAATTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAAGTGCCAGGCCAGTGAAAGCATTAGCAACTACTTATCCTGGTATCAGCAGATTCCAGGGAAAGTTCCTAAGCTCCTGATCTATTATGCATCCAATCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAGCTATTATGGTGGTGGTAGTGCCTATACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 90:PL004B9 scFv aa
EVQLVESGGGLVQPGGSLRLSCTVSGIDLSVINMGWVRQAPGKGLEWIGTITYVGNTYYASWAKGRFTISKTSTTVDLKITSPTTEDTAVYYCARESGTIYYSYFNLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSAFELTQSPSSLSASVGDRVTIKCQASESISNYLSWYQQIPGKVPKLLIYYASNLASGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCQSYYGGGSAYTFGGGTKVEIK
SEQ ID 91:CT4 VH nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCA
CCTTCAGTAGCTATACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGACATTTATATCATATGAT
GGAAACAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCT
GCAAATGAACAGCCTGAGAGCTGAGGACACGGCTATATATTACTGTGCGAGGACCGGCTGGCTGGGGCCCTTTGACTACT
GGGGCCAGGGAACCCTGGTCACCGTCTCCTCA
SEQ ID 92:CT4 VH aa
QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYTMHWVRQAPGKGLEWVTFISYDGNNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAIYYCARTGWLGPFDYWGQGTLVTVSS
SEQ ID 93:CT4 VL nt
GAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA
SEQ ID 94:CT4 VL aa
EIVLTQSPGTLSLSPGERATLSCRASQSVGSSYLAWYQQKPGQAPRLLIYGAFSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIK
SEQ ID 95:PD224D1 VH nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCTCCCTCAGTAGCTATGCAATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTACATCGGCTACATTGGTGATACTACTGGCATAGCCTACGCGAGCTGGGCGAATGGCAGATTCACCATCTCCAAAGACAATACCAAGAACACGGTGGATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGAGGCTGGTCCTACTTAGACATCTGGGGCCAAGGGACCCTGGTCACCGTCTCGAGC
SEQ ID 96:PD224D1 VH aa
EVQLVESGGGLVQPGGSLRLSCTASGFSLSSYAMSWVRQAPGKGLEYIGYIGDTTGIAYASWANGRFTISKDNTKNTVDL
QMNSLRAEDTAVYYCARGWSYLDIWGQGTLVTVSS
SEQ ID 97:PD224D1 VL nt
GCCCTTGTGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAACATTTACAGCAATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATCAGGCCTCCACTCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATATGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAGGCGGTTATTATAGTGCTGCCCTTAATACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 98:PD224D1 VL aa
ALVMTQSPSSLSASVGDRVTITCQASQNIYSNLAWYQQKPGKVPKLLIYQASTLASGVPSRFSGSGYGTDFTLTISSLQP
EDVATYYCQGGYYSAALNTFGGGTKVEIK
SEQ ID 99:PD206F12 VH nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCGACTTCAGTAGCGGCTACTGGATATGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTTGATCGCATGCATTTATGCTGGTACTAGTGGTAGTACTTCCTACGCGAGCTGGGCGAGAGGCAGATTCACCATCTCCGAAACCTCCAAGAACACGGTGACTCTTCAAATGAACAGCCTGAGAGCCGAGGACTCGGCTGTGTATTACTGTGCGAGAAATCTTTACACTTACAATAGCTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGC
SEQ ID 100:PD206F12 VH aa
EVQLVESGGGLVQPGGSLRLSCAASGFDFSSGYWICWVRQAPGKGLELIACIYAGTSGSTSYASWARGRFTISETSKNTVTLQMNSLRAEDSAVYYCARNLYTYNSLWGQGTLVTVSS
SEQ ID 101:PD206F12 VL nt
GACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCAGAGTGTTTATGATAACAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATACAGTATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAGGCACTTATTATAGTAGTGGTTGGAACTTTGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 102:PD206F12 VL aa
DIQMTQSPSTLSASVGDRVTITCQSSQSVYDNNWLAWYQQKPGKAPKLLIYTVSTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQGTYYSSGWNFAFGGGTKVEIK
SEQ ID 103:PD215A1 VH nt
CAGGAGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGCCTCTGGATTCTCCTTTAGCAGCTACTGGATGTGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGATGCATTACGACTGGTAGTGGTAGCACTTACTACGCGAGCTGGGCGAAGCGCCGGTTCACCATCTCCAAAGACAATTCCAAGAACACGGTGACTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTACGAGAGCATTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGC
SEQ ID 104:PD215A1 VH aa
QEQLLESGGGLVQPGGSLRLSCTASGFSFSSYWMCWVRQAPGKGLEWIGCITTGSGSTYYASWAKRRFTISKDNSKNTVTLQMNSLRAEDTAVYYCTRAFDLWGQGTLVTVSS
SEQ ID 105:PD215A1 VL nt
GACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAGCATTTACAGCTACTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCAATCTGGCATCTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAAGCAGTTGGTTGAGTGGTGCTGTTGGTAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 106:PD215A1 VL aa
DIQMTQSPSSLSASVGDRVTITCQASQSIYSYLNWYQQKPGKAPKLLIYGASNLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQSSWLSGAVGNAFGGGTKVEIK
SEQ ID 107:PD225G11 VH nt
GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCAGCCACTGGATATGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGCATGCATTTATACTGGTAGTATTGATGTCTTTTACTACGCGAGCTGGGCGAAAGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGAGCCGCTAATACTGATACTACCTACTTTAACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGC
SEQ ID 108:PD225G11 VH aa
EVQLLESGGGLVQPGGSLRLSCAASGFTFSSSHWICWVRQAPGKGLEWIACIYTGSIDVFYYASWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARAANTDTTYFNLWGQGTLVTVSS
SEQ ID 109:PD225G11 VL nt
GCCTATGATATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGCCAGGCCAGTCAGAGCATTAACAACCAACTATCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATGGTGCATCCACTCTGGCATCTGGGGTCCCATCTCGGTTCACCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCATGTTCATTATTGCAGTGGTGGTAGTTGTTTTTGGGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 110:PD225G11 VL aa
AYDMTQSPSSLSASVGDRVTINCQASQSINNQLSWYQQKPGKVPKLLIYGASTLASGVPSRFTGSGSGTDFTLTISSLQPEDVATYYCHVHYCSGGSCFWAFGGGTKVEIK
SEQ ID 111:PL230C6 VH nt
CAGTCGGTGGAGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACCGCCTCTGGAATCGACCTTAATACCTACGACATGATCTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTTGGAATCATTACTTATAGTGGTAGTAGATACTACGCGAACTGGGCGAAAGGCCGATTCACCATCTCCAAAGACAATACCAAGAACACGGTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATTATATGAGTGGTTCCCACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCC
SEQ ID 112:PL230C6 VH aa
QSVEESGGGLVQPGGSLRLSCTASGIDLNTYDMIWVRQAPGKGLEWVGIITYSGSRYYANWAKGRFTISKDNTKNTVYLQMNSLRAEDTAVYYCARDYMSGSHLWGQGTLVTVSS
SEQ ID 113:PL230C6 VL nt
GCCTATGATATGACCCAGTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCAAGTGTCAGGCCAGTGAGGACATTTATAGCTTCTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATTCTGCATCCTCTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTTATGGTAAAAATAATGTTGATAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 114:PL230C6 VL aa
AYDMTQSPSSVSASVGDRVTIKCQASEDIYSFLAWYQQKPGKAPKLLIHSASSLASGVPSRFSGSGSGTDFTLTISSLQP
EDFATYYCQQGYGKNNVDNAFGGGTKVEIK
SEQ ID 115:PL231H2 VH nt
CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTCCAGCCTGGGGGCTCCCTGAGACTCTCCTGTACAACTTCTGGAATCGACCTTAGTACCTACGACATGATCTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGGAATCATTAGTTATGTTGGTAACACATACTACGCGAGCTGGGCGAAAGGCCGATTCACCCTCTCCAAAGACAATACCTCGACCACGGTGGATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGATTTTATTAGTGGTTCCCACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGC
SEQ ID 116:PL231H2 VH aa
QVQLVESGGGLVQPGGSLRLSCTTSGIDLSTYDMIWVRQAPGKGLEWVGIISYVGNTYYASWAKGRFTLSKDNTSTTVDLQMNSLRAEDTAVYYCARDFISGSHLWGQGTLVTVSS
SEA ID 117:PL231H2 VL nt
GCCTATGATATGACCCAGTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCAATTGTCAGGCCAGTGAGAGCATTAGTAGCTTCTTATCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTTCTGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTTATAGTAAAAGTAATGTGGATAATGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 118:PL231H2 VL aa
AYDMTQSPSSVSASVGDRVTINCQASESISSFLSWYQQKPGKAPKLLIFSASTLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGYSKSNVDNAFGGGTKVEIK
SEQ ID 119:PL221G5 VH nt
GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCTTCAGTAGCGGGTACGACATGTGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGCATGCATTGCTGCTGGTAGTGCTGGTATCACTTACGACGCGAACTGGGCGAAAGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGATCGGCGTTTTCGTTCGACTACGCCATGGACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGC
SEA ID 120:PL221G5 VH aa
EVQLLESGGGLVQPGGSLRLSCAASGFSFSSGYDMCWVRQAPGKGLEWIACIAAGSAGITYDANWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARSAFSFDYAMDLWGQGTLVTVSS
SEQ ID 121:PL221G5 VL nt
GACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCCAGTCAGAGCATTAGTTCCCACTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTTACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGGGTTATAGTTGGGGTAATGTTGATAATGTTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEA ID 122:PL221G5 VL aa
DIQMTQSPSTLSASVGDRVTITCQASQSISSHLNWYQQKPGKAPKLLIYKASTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQQGYSWGNVDNVFGGGTKVEIK
SEA ID 123:PL004B5 VH nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCTCCCTCAGTAGCTACTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGAGTCATTGATACTAATGTTTATATATACTACGCGAACTGGGCAAAAGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTTCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGATATGTGGGTAATAATGATGATTATATTAACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGC
SEQ ID 124:PL004B5 VH aa
EVQLVESGGGLVQPGGSLRLSCAASGFSLSSYWMSWVRQAPGKGLEWIGVIDTNVYIYYANWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARYVGNNDDYINLWGQGTLVTVSS
SEQ ID 125:PL004B5 VL nt
GACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCAGAGTGTTTATAATGGCTACTGGTTATCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCACTCTGGCATCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCTAGGCAGTTATACTAGTAGTACTGAGAACTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 126:PL004B5 VL aa
DIQMTQSPSTLSASVGDRVTITCQSSQSVYNGYWLSWYQQKPGKAPKLLIYGASTLASGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCLGSYTSSTENSFGGGTKVEIK
SEA ID 127:PL004B9 VH nt
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTACAGTGTCTGGAATCGACCTCAGTGTCATCAATATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATCGGAACCATTACTTATGTTGGTAACACATATTACGCGAGCTGGGCGAAAGGCAGATTCACCATCTCCAAAACCTCGACCACGGTGGATCTTAAAATCACCAGTCCGACAACCGAGGACACGGCTGTGTATTACTGTGCGAGAGAATCTGGTACTATTTATTACAGTTACTTTAACTTGTGGGGCCAAGGGACCCTGGTCACCGTCTCGAGC
SEQ ID 128:PL004B9 VH aa
EVQLVESGGGLVQPGGSLRLSCTVSGIDLSVINMGWVRQAPGKGLEWIGTITYVGNTYYASWAKGRFTISKTSTTVDLKITSPTTEDTAVYYCARESGTIYYSYFNLWGQGTLVTVSS
SEQ ID 129:PL004B9 VL nt
GCATTCGAATTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAAGTGCCAGGCCAGTGAAAGCATTAGCAACTACTTATCCTGGTATCAGCAGATTCCAGGGAAAGTTCCTAAGCTCCTGATCTATTATGCATCCAATCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAGCTATTATGGTGGTGGTAGTGCCTATACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 130:PL004B9 VL aa
AFELTQSPSSLSASVGDRVTIKCQASESISNYLSWYQQIPGKVPKLLIYYASNLASGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCQSYYGGGSAYTFGGGTKVEIK
SEQ ID 131:PD220F6 VH nt
CAGGAGCAGGTGAAGGAGACCGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATCAGCAGCTATGGAGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCGCATTGATTTTTCCCGGGATTGGTTTCAAAGACTACGCGAGCTGGGTGAATGGCCGGTTCACCCTCTCCAGCGACAACGCCCAGAACACTGTGGAACTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGAGATTTGGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGC
SEQ ID 132:PD220F6 VH aa
QEQVKETGGGLVQPGGSLRLSCAASGFTISSYGVSWVRQAPGKGLEWVALIFPGIGFKDYASWVNGRFTLSSDNAQNTVELQMNSLRAEDTAVYYCARDLDLWGQGTLVTVSS
SEQ ID 133:PD220F6 VL nt
GACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCCGAGTGTTTATAGTAACTACTTATCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATTATGCATCCACTCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTGCAGGCGGTTATAGTAGTAGTACTCGTGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 134:PD220F6 VL aa
DIQMTQSPSSLSASVGDRVTITCQSSPSVYSNYLSWYQQKPGKVPKLLIYYASTLASGVPSRFSGSGSGTDFTLTI SSLQPEDVATYYCAGGYSSSTRAFGGGTKVEIK
SEQ ID 135:人IgG1恒定区nt
GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGT
SEQ ID 136:人IgG1恒定区aa
ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG
SEQ ID 137:人IgG1效应子空白nt
GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCCGCGGGGGCACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCGCGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGT
SEQ ID 138:人IgG1效应子空白aa
ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCAVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG
SEQ ID 139:PD220F6 scFv nt
CAGGAGCAGGTGAAGGAGACCGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATCAGCAGCTATGGAGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCGCATTGATTTTTCCCGGGATTGGTTTCAAAGACTACGCGAGCTGGGTGAATGGCCGGTTCACCCTCTCCAGCGACAACGCCCAGAACACTGTGGAACTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGAGATTTGGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCGAGCGGTGGAGGCGGATCTGGCGGAGGTGGTTCCGGCGGTGGCGGCTCCGGTGGAGGCGGCTCTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGTCCAGTCCGAGTGTTTATAGTAACTACTTATCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATTATGCATCCACTCTGGCATCTGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTGCAGGCGGTTATAGTAGTAGTACTCGTGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA
SEQ ID 140:PD220F6 scFv aa
QEQVKETGGGLVQPGGSLRLSCAASGFTISSYGVSWVRQAPGKGLEWVALIFPGIGFKDYASWVNGRFTLSSDNAQNTVELQMNSLRAEDTAVYYCARDLDLWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSDIQMTQSPSSLSASVGDRVTITCQSSPSVYSNYLSWYQQKPGKVPKLLIYYASTLASGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCAGGYSSSTRAFGGGTKVEIK
SEQ ID 141:CT4 VH CDR1 aa
SSYTMH
SEQ ID 142:CT4 VH CDR2 aa
FISYDGNNKYYADSVKG
SEQ ID 143:CT4 VH CDR3 aa
TGWLGPFDY
SEQ ID 144:CT4 VL CDR1 aa
RASQSVGSSYLA
SEQ ID 145:CT4 VL CDR2 aa
GAFSRAT
SEQ ID 146:CT4 VL CDR3 aa
QQYGSSPWT
SEQ ID 147:PL230C6 VH CDR1 aa
NTYDMI
SEQ ID 148:PL230C6VH CDR2 aa
IITYSGSRYYANWAKG
SEQ ID 149:PL230C6VH CDR3 aa
DYMSGSHL
SEQ ID 150:PL230C6VL CDR1 aa
QASEDIYSFLA
SEQ ID 151:PL230C6VL CDR2 aa
SASSLAS
SEQ ID 152:PL230C6VL CDR3 aa
QQGYGKNNVDNA
SEQ ID 153:PD224D1 VH CDR1 aa
NTYDMI
SEQ ID 154:PD224D1 CDR2 aa
IITYSGSRYYANWAKG
SEQ ID 155:PD224D1 CDR3 aa
DYMSGSHL
SEQ ID 156:PD224D1 CDR1 aa
QASEDIYSFLA
SEQ ID 157:PD224D1 CDR2 aa
SASSLAS
SEQ ID 158:PD224D1 CDR3 aa
QQGYGKNNVDNA
SEQ ID 159:PL221G5 VH CDR1 aa
SGYDMC
SEQ ID 160:PL221G5 CDR2 aa
CIAAGSAGITYDANWAKG
SEQ ID 161:PL221G5 CDR3 aa
SAFSFDYAMDL
SEQ ID 162:PL221G5 CDR1 aa
QASQSISSHLN
SEQ ID 163:PL221G5 CDR2 aa
KASTLAS
SEQ ID 164:PL221G5 CDR3 aa
QQGYSWGNVDNV
SEQ ID 165:PL231H2 VH CDR1 aa
STYDMI
SEQ ID 166:PL231H2 VH CDR2 aa
IISYVGNTYYASWAKG
SEQ ID 167:PL231H2 VH CDR3 aa
DFISGSHL
SEQ ID 168:PL231H2 VL CDR1 aa
QASESISSFLS
SEQ ID 169:PL231H2 VL CDR2 aa
SASTLAS
SEQ ID 170:PL231H2 VL CDR3 aa
QQGYSKSNVDNA
SEQ ID 171:PL004B5 VH CDR1 aa
SSYWMS
SEQ ID 172:PL004B5 VH CDR2 aa
VIDTNVYIYYANWAKG
SEQ ID 173:PL004B5 VH CDR3 aa
YVGNNDDYINL
SEQ ID 174:PL004B5 VL CDR1 aa
QSSQSVYNGWLS
SEQ ID 175:PL004B5 VL CDR2 aa
GASTLAS
SEQ ID 176:PL004B5 VL CDR3 aa
LGSYTSSTENS
SEQ ID 177:PL004B9 VH CDR1 aa
SVINMG
SEQ ID 178:PL004B9 VH CDR2 aa
TITYVGNTYYASWAKG
SEQ ID 179:PL004B9 VH CDR3 aa
ESGTIYYSYFNL
SEQ ID 180:PL004B9 VL CDR1 aa
QASESISNYLS
SEQ ID 181:PL004B9 VL CDR2 aa
YASNLAS
SEQ ID 182:PL004B9 VL CDR3 aa
QSYYGGGSAYT
SEQ ID 183:PD206F12 VH CDR1 aa
SGYWIC
SEQ ID 184:PD206F12 VH CDR2 aa
CIYAGTSGSTSYASWARG
SEQ ID 185:PD206F12 VH CDR3 aa
NLYTYNSL
SEQ ID 186:PD206F12 VL CDR1 aa
QSSQSVYDNNWLA
SEQ ID 187:PD206F12 VL CDR2 aa
TVSTLAS
SEQ ID 188:PD206F12 VL CDR3 aa
QGTYYSSGWNFA
SEQ ID 189:PD215A1 VH CDR1 aa
SSYWMC
SEQ ID 190:PD215A1 VH CDR2 aa
CITTGSGSTYYASWAKR
SEQ ID 191:PD215A1 VH CDR3 aa
AFDL
SEQ ID 192:PD215A1 VL CDR1 aa
QASQSIYSYLN
SEQ ID 193:PD215A1 VL CDR2 aa
GASNLAS
SEQ ID 194:PD215A1 VL CDR3 aa
QSSWLSGAVGNA
SEQ ID 195:PD220F6 VH CDR1 aa
SSYGVS
SEQ ID 196:PD220F6 VH CDR2 aa
LIFPGIGFKDYASWVNG
SEQ ID 197:PD220F6 VH CDR3 aa
DLDL
SEQ ID 198:PD220F6 VL CDR1 aa
QSSPSVYSNYLS
SEQ ID 199:PD220F6 VL CDR2 aa
YASTLAS
SEQ ID 200:PD220F6 VL CDR3 aa
AGGYSSSTRA
SEQ ID 201:PD225G11 VH CDR1 aa
SSHWIC
SEQ ID 202:PD225G11 VH CDR2 aa
CIYTGSIDVFYYASWAKG
SEQ ID 203:PD225G11 VH CDR3 aa
AANTDTTYFNL
SEQ ID 204:PD225G11 VL CDR1 aa
QASQSINNQLS
SEQ ID 205:PD225G11 VL CDR2 aa
GASTLAS
SEQ ID 206:PD225G11 VL CDR3 aa
HVHYCSGGSCFWA
Figure BDA0002408401030000631
序列表
<110> 西雅图免疫公司
四川百利药业有限责任公司
<120> 双特异性抗体及其制备和使用方法
<130> I190370CN
<140> 2018800591309
<141> 2018-11-01
<150> 62/580,845
<151> 2017-11-02
<160> 206
<170> SIPOSequenceListing 1.0
<210> 1
<211> 2103
<212> DNA
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 1
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc cgaggtgcag 1380
ctggtggagt ctgggggagg cttggtccag cctggggggt ccctgagact ctcctgtaca 1440
gcctctggat tctccctcag tagctatgca atgagctggg tccgccaggc tccagggaag 1500
gggctggagt acatcggcta cattggtgat actactggca tagcctacgc gagctgggcg 1560
aatggcagat tcaccatctc caaagacaat accaagaaca cggtggatct tcaaatgaac 1620
agcctgagag ccgaggacac ggctgtgtat tactgtgcga gaggctggtc ctacttagac 1680
atctggggcc aagggaccct ggtcaccgtc tcgagcggtg gaggcggatc tggcggaggt 1740
ggttccggcg gtggcggctc cggtggaggc ggctctgccc ttgtgatgac ccagtctcca 1800
tcctccctgt ctgcatctgt aggagacaga gtcaccatca cttgccaggc cagtcagaac 1860
atttacagca atttagcctg gtatcagcag aaaccaggga aagttcctaa gctcctgatc 1920
tatcaggcct ccactctggc atctggggtc ccatctcggt tcagtggcag tggatatggg 1980
acagatttca ctctcaccat cagcagcctg cagcctgaag atgttgcaac ttattactgt 2040
caaggcggtt attatagtgc tgcccttaat actttcggcg gagggaccaa ggtggagatc 2100
aaa 2103
<210> 2
<211> 701
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 2
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser
450 455 460
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr
465 470 475 480
Ala Ser Gly Phe Ser Leu Ser Ser Tyr Ala Met Ser Trp Val Arg Gln
485 490 495
Ala Pro Gly Lys Gly Leu Glu Tyr Ile Gly Tyr Ile Gly Asp Thr Thr
500 505 510
Gly Ile Ala Tyr Ala Ser Trp Ala Asn Gly Arg Phe Thr Ile Ser Lys
515 520 525
Asp Asn Thr Lys Asn Thr Val Asp Leu Gln Met Asn Ser Leu Arg Ala
530 535 540
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly Trp Ser Tyr Leu Asp
545 550 555 560
Ile Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly
565 570 575
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
580 585 590
Ala Leu Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
595 600 605
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Asn Ile Tyr Ser Asn
610 615 620
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile
625 630 635 640
Tyr Gln Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
645 650 655
Ser Gly Tyr Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
660 665 670
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gly Gly Tyr Tyr Ser Ala Ala
675 680 685
Leu Asn Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 3
<211> 645
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 3
gaaattgtgt tgacgcagtc tccaggcacc ctgtctttgt ctccagggga aagagccacc 60
ctctcctgca gggccagtca gagtgttggc agcagctact tagcctggta ccagcagaaa 120
cctggccagg ctcccaggct cctcatctat ggtgcattca gcagggccac tggcatccca 180
gacaggttca gtggcagtgg gtctgggaca gacttcactc tcaccatcag cagactggag 240
cctgaagatt ttgcagtgta ttactgtcag cagtatggta gctcaccgtg gacgttcggc 300
caagggacca aggtggaaat caaacgtacg gtggctgcac catctgtctt catcttcccg 360
ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc 420
tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc 480
caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg 540
acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag 600
ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgt 645
<210> 4
<211> 215
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 4
Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly
1 5 10 15
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser Ser
20 25 30
Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu
35 40 45
Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser
50 55 60
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu
65 70 75 80
Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro
85 90 95
Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala
100 105 110
Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser
115 120 125
Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu
130 135 140
Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser
145 150 155 160
Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu
165 170 175
Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val
180 185 190
Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys
195 200 205
Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 5
<211> 2106
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 5
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc ccagtcggtg 1380
gaggagtctg ggggaggctt ggtccagcct ggggggtccc tgagactctc ctgtaccgcc 1440
tctggaatcg accttaatac ctacgacatg atctgggtcc gccaggctcc aggcaagggg 1500
ctagagtggg ttggaatcat tacttatagt ggtagtagat actacgcgaa ctgggcgaaa 1560
ggccgattca ccatctccaa agacaatacc aagaacacgg tgtatctgca aatgaacagc 1620
ctgagagctg aggacacggc tgtgtattac tgtgcgagag attatatgag tggttcccac 1680
ttgtggggcc agggaaccct ggtcaccgtc tcttccggtg gaggcggttc aggcggaggt 1740
ggaagtggtg gtggcggctc tggaggcggc ggatctgcct atgatatgac ccagtctcca 1800
tcttccgtgt ctgcatctgt aggagacaga gtcaccatca agtgtcaggc cagtgaggac 1860
atttatagct tcttggcctg gtatcagcag aaaccaggga aagcccctaa gctcctgatc 1920
cattctgcat cctctctggc atctggggtc ccatcaaggt tcagcggcag tggatctggg 1980
acagatttca ctctcaccat cagcagcctg cagcctgaag attttgcaac ttactattgt 2040
caacagggtt atggtaaaaa taatgttgat aatgctttcg gcggagggac caaggtggag 2100
atcaaa 2106
<210> 6
<211> 702
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 6
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Glu Glu Ser Gly
450 455 460
Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala
465 470 475 480
Ser Gly Ile Asp Leu Asn Thr Tyr Asp Met Ile Trp Val Arg Gln Ala
485 490 495
Pro Gly Lys Gly Leu Glu Trp Val Gly Ile Ile Thr Tyr Ser Gly Ser
500 505 510
Arg Tyr Tyr Ala Asn Trp Ala Lys Gly Arg Phe Thr Ile Ser Lys Asp
515 520 525
Asn Thr Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu
530 535 540
Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Met Ser Gly Ser His
545 550 555 560
Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly
565 570 575
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
580 585 590
Ala Tyr Asp Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly
595 600 605
Asp Arg Val Thr Ile Lys Cys Gln Ala Ser Glu Asp Ile Tyr Ser Phe
610 615 620
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
625 630 635 640
His Ser Ala Ser Ser Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
645 650 655
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
660 665 670
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Tyr Gly Lys Asn Asn
675 680 685
Val Asp Asn Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 7
<211> 2127
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 7
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc cgaggtgcag 1380
ctgttggagt ctgggggagg cttggtacag cctggggggt ccctgagact ctcctgtgca 1440
gcctctggat tctccttcag tagcgggtac gacatgtgct gggtccgcca ggctccaggg 1500
aaggggctgg agtggatcgc atgcattgct gctggtagtg ctggtatcac ttacgacgcg 1560
aactgggcga aaggccggtt caccatctcc agagacaatt ccaagaacac gctgtatctg 1620
caaatgaaca gcctgagagc cgaggacacg gccgtatatt actgtgcgag atcggcgttt 1680
tcgttcgact acgccatgga cctctggggc cagggaaccc tggtcaccgt ctcgagcggt 1740
ggaggcggat ctggcggagg tggttccggc ggtggcggct ccggtggagg cggctctgac 1800
atccagatga cccagtctcc ttccaccctg tctgcatctg taggagacag agtcaccatc 1860
acttgccagg ccagtcagag cattagttcc cacttaaact ggtatcagca gaaaccaggg 1920
aaagccccta agctcctgat ctataaggca tccactctgg catctggggt cccatcaagg 1980
ttcagcggca gtggatctgg gacagaattt actctcacca tcagcagcct gcagcctgat 2040
gattttgcaa cttattactg ccaacagggt tatagttggg gtaatgttga taatgttttc 2100
ggcggaggga ccaaggtgga gatcaaa 2127
<210> 8
<211> 709
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 8
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Leu Glu Ser
450 455 460
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala
465 470 475 480
Ala Ser Gly Phe Ser Phe Ser Ser Gly Tyr Asp Met Cys Trp Val Arg
485 490 495
Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Ala Cys Ile Ala Ala Gly
500 505 510
Ser Ala Gly Ile Thr Tyr Asp Ala Asn Trp Ala Lys Gly Arg Phe Thr
515 520 525
Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser
530 535 540
Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Ala Phe
545 550 555 560
Ser Phe Asp Tyr Ala Met Asp Leu Trp Gly Gln Gly Thr Leu Val Thr
565 570 575
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
580 585 590
Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser
595 600 605
Thr Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Gln Ala
610 615 620
Ser Gln Ser Ile Ser Ser His Leu Asn Trp Tyr Gln Gln Lys Pro Gly
625 630 635 640
Lys Ala Pro Lys Leu Leu Ile Tyr Lys Ala Ser Thr Leu Ala Ser Gly
645 650 655
Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu
660 665 670
Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln
675 680 685
Gln Gly Tyr Ser Trp Gly Asn Val Asp Asn Val Phe Gly Gly Gly Thr
690 695 700
Lys Val Glu Ile Lys
705
<210> 9
<211> 2109
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 9
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc acaggtgcag 1380
ctggtggagt ctgggggagg cctggtccag cctgggggct ccctgagact ctcctgtaca 1440
acttctggaa tcgaccttag tacctacgac atgatctggg tccgccaggc tccaggcaag 1500
gggctagagt gggtgggaat cattagttat gttggtaaca catactacgc gagctgggcg 1560
aaaggccgat tcaccctctc caaagacaat acctcgacca cggtggatct gcaaatgaac 1620
agcctgagag ctgaggacac ggctgtgtat tactgtgcga gagattttat tagtggttcc 1680
cacttgtggg gccagggaac cctggtcacc gtctcgagcg gtggaggcgg atctggcgga 1740
ggtggttccg gcggtggcgg ctccggtgga ggcggttctg cctatgatat gacccagtct 1800
ccatcttccg tgtctgcatc tgtaggagac agagtcacca tcaattgtca ggccagtgag 1860
agcattagta gcttcttatc ctggtatcag cagaaaccag ggaaagcccc taagctcctg 1920
atcttttctg catccactct ggcatctggg gtcccatcaa ggttcagcgg cagtggatct 1980
gggacagatt tcactctcac catcagcagc ctgcagcctg aagattttgc aacttactat 2040
tgtcaacagg gttatagtaa aagtaatgtg gataatgctt tcggcggagg gaccaaggtg 2100
gagatcaaa 2109
<210> 10
<211> 703
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 10
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser
450 455 460
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr
465 470 475 480
Thr Ser Gly Ile Asp Leu Ser Thr Tyr Asp Met Ile Trp Val Arg Gln
485 490 495
Ala Pro Gly Lys Gly Leu Glu Trp Val Gly Ile Ile Ser Tyr Val Gly
500 505 510
Asn Thr Tyr Tyr Ala Ser Trp Ala Lys Gly Arg Phe Thr Leu Ser Lys
515 520 525
Asp Asn Thr Ser Thr Thr Val Asp Leu Gln Met Asn Ser Leu Arg Ala
530 535 540
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Phe Ile Ser Gly Ser
545 550 555 560
His Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly
565 570 575
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
580 585 590
Ser Ala Tyr Asp Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val
595 600 605
Gly Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Glu Ser Ile Ser Ser
610 615 620
Phe Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
625 630 635 640
Ile Phe Ser Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser
645 650 655
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
660 665 670
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Tyr Ser Lys Ser
675 680 685
Asn Val Asp Asn Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 11
<211> 2121
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 11
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc agaggtgcag 1380
ctggtggagt ctgggggagg cttggtccag cctggggggt ccctgagact ctcctgtgca 1440
gcctctggat tctccctcag tagctactgg atgagctggg tccgccaggc tccagggaag 1500
gggctggagt ggatcggagt cattgatact aatgtttata tatactacgc gaactgggca 1560
aaaggcagat tcaccatctc cagagacaat tccaagaaca cgctgtatct tcaaatgaac 1620
agcctgagag ccgaggacac ggctgtgtat tactgtgcga gatatgtggg taataatgat 1680
gattatatta acttgtgggg ccagggaacc ctggtcaccg tctcgagcgg tggaggcgga 1740
tctggcggag gtggttccgg cggtggcggc tccggtggag gcggctctga catccagatg 1800
acccagtctc cttccaccct gtctgcatct gtaggagaca gagtcaccat cacttgccag 1860
tccagtcaga gtgtttataa tggctactgg ttatcctggt atcagcagaa accagggaaa 1920
gcccctaagc tcctgatcta tggtgcatcc actctggcat ctggggtccc atcaaggttc 1980
agcggcagtg gatctgggac agaattcact ctcaccatca gcagcctgca gcctgatgat 2040
tttgcaactt attactgcct aggcagttat actagtagta ctgagaactc tttcggcgga 2100
gggaccaagg tggagatcaa a 2121
<210> 12
<211> 707
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 12
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser
450 455 460
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala
465 470 475 480
Ala Ser Gly Phe Ser Leu Ser Ser Tyr Trp Met Ser Trp Val Arg Gln
485 490 495
Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Val Ile Asp Thr Asn Val
500 505 510
Tyr Ile Tyr Tyr Ala Asn Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg
515 520 525
Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala
530 535 540
Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Tyr Val Gly Asn Asn Asp
545 550 555 560
Asp Tyr Ile Asn Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
565 570 575
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
580 585 590
Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser
595 600 605
Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Gln Ser
610 615 620
Val Tyr Asn Gly Tyr Trp Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys
625 630 635 640
Ala Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val
645 650 655
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr
660 665 670
Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Leu Gly
675 680 685
Ser Tyr Thr Ser Ser Thr Glu Asn Ser Phe Gly Gly Gly Thr Lys Val
690 695 700
Glu Ile Lys
705
<210> 13
<211> 2112
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 13
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc agaggtgcag 1380
ctggtggagt ctgggggagg cttggtccag cctggggggt ccctgagact ctcctgtaca 1440
gtgtctggaa tcgacctcag tgtcatcaat atgggctggg tccgccaggc tccagggaag 1500
gggctggagt ggatcggaac cattacttat gttggtaaca catattacgc gagctgggcg 1560
aaaggcagat tcaccatctc caaaacctcg accacggtgg atcttaaaat caccagtccg 1620
acaaccgagg acacggctgt gtattactgt gcgagagaat ctggtactat ttattacagt 1680
tactttaact tgtggggcca agggaccctg gtcaccgtct cgagcggtgg aggcggatct 1740
ggcggaggtg gttccggcgg tggcggctcc ggtggaggcg gctctgcatt cgaattgacc 1800
cagtctccat cctccctgtc tgcatctgta ggagacagag tcaccatcaa gtgccaggcc 1860
agtgaaagca ttagcaacta cttatcctgg tatcagcaga ttccagggaa agttcctaag 1920
ctcctgatct attatgcatc caatctggca tctggggtcc catctcggtt cagtggcagt 1980
ggatctggga cagatttcac tctcaccatc agcagcctgc agcctgaaga tgttgcaact 2040
tattactgtc aaagctatta tggtggtggt agtgcctata ctttcggcgg agggaccaag 2100
gtggagatca aa 2112
<210> 14
<211> 704
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 14
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser
450 455 460
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr
465 470 475 480
Val Ser Gly Ile Asp Leu Ser Val Ile Asn Met Gly Trp Val Arg Gln
485 490 495
Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Thr Ile Thr Tyr Val Gly
500 505 510
Asn Thr Tyr Tyr Ala Ser Trp Ala Lys Gly Arg Phe Thr Ile Ser Lys
515 520 525
Thr Ser Thr Thr Val Asp Leu Lys Ile Thr Ser Pro Thr Thr Glu Asp
530 535 540
Thr Ala Val Tyr Tyr Cys Ala Arg Glu Ser Gly Thr Ile Tyr Tyr Ser
545 550 555 560
Tyr Phe Asn Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly
565 570 575
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
580 585 590
Gly Gly Ser Ala Phe Glu Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala
595 600 605
Ser Val Gly Asp Arg Val Thr Ile Lys Cys Gln Ala Ser Glu Ser Ile
610 615 620
Ser Asn Tyr Leu Ser Trp Tyr Gln Gln Ile Pro Gly Lys Val Pro Lys
625 630 635 640
Leu Leu Ile Tyr Tyr Ala Ser Asn Leu Ala Ser Gly Val Pro Ser Arg
645 650 655
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser
660 665 670
Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Ser Tyr Tyr Gly
675 680 685
Gly Gly Ser Ala Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 15
<211> 2121
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 15
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc agaggtgcag 1380
ctggtggagt ctgggggagg cttggtccag cctggggggt ccctgagact ctcctgtgca 1440
gcctctggat tcgacttcag tagcggctac tggatatgct gggtccgcca ggctccaggg 1500
aaggggctgg agttgatcgc atgcatttat gctggtacta gtggtagtac ttcctacgcg 1560
agctgggcga gaggcagatt caccatctcc gaaacctcca agaacacggt gactcttcaa 1620
atgaacagcc tgagagccga ggactcggct gtgtattact gtgcgagaaa tctttacact 1680
tacaatagct tgtggggcca gggaaccctg gtcaccgtct cgagcggtgg aggcggatct 1740
ggcggaggtg gttccggcgg tggcggctcc ggtggaggcg gctctgacat ccagatgacc 1800
cagtctcctt ccaccctgtc tgcatctgta ggagacagag tcaccatcac ttgccagtcc 1860
agtcagagtg tttatgataa caactggtta gcctggtatc agcagaaacc agggaaagcc 1920
cctaagctcc tgatctatac agtatccact ctggcatctg gggtcccatc aaggttcagc 1980
ggcagtggat ctgggacaga attcactctc accatcagca gcctgcagcc tgatgatttt 2040
gcaacttatt actgccaagg cacttattat agtagtggtt ggaactttgc tttcggcgga 2100
gggaccaagg tggagatcaa a 2121
<210> 16
<211> 707
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 16
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser
450 455 460
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala
465 470 475 480
Ala Ser Gly Phe Asp Phe Ser Ser Gly Tyr Trp Ile Cys Trp Val Arg
485 490 495
Gln Ala Pro Gly Lys Gly Leu Glu Leu Ile Ala Cys Ile Tyr Ala Gly
500 505 510
Thr Ser Gly Ser Thr Ser Tyr Ala Ser Trp Ala Arg Gly Arg Phe Thr
515 520 525
Ile Ser Glu Thr Ser Lys Asn Thr Val Thr Leu Gln Met Asn Ser Leu
530 535 540
Arg Ala Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Asn Leu Tyr Thr
545 550 555 560
Tyr Asn Ser Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly
565 570 575
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
580 585 590
Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala
595 600 605
Ser Val Gly Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Gln Ser Val
610 615 620
Tyr Asp Asn Asn Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala
625 630 635 640
Pro Lys Leu Leu Ile Tyr Thr Val Ser Thr Leu Ala Ser Gly Val Pro
645 650 655
Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile
660 665 670
Ser Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gly Thr
675 680 685
Tyr Tyr Ser Ser Gly Trp Asn Phe Ala Phe Gly Gly Gly Thr Lys Val
690 695 700
Glu Ile Lys
705
<210> 17
<211> 2100
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 17
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc acaggagcag 1380
ctgttggagt ctgggggagg cttggtacag cctggggggt ccctgagact ctcctgtaca 1440
gcctctggat tctcctttag cagctactgg atgtgctggg tccgccaggc tccagggaag 1500
gggctggagt ggatcggatg cattacgact ggtagtggta gcacttacta cgcgagctgg 1560
gcgaagcgcc ggttcaccat ctccaaagac aattccaaga acacggtgac tctgcaaatg 1620
aacagcctga gagccgagga cacggccgta tattactgta cgagagcatt tgacttgtgg 1680
ggccagggaa ccctggtcac cgtctcgagc ggtggaggcg gatctggcgg aggtggttcc 1740
ggcggtggcg gctccggtgg aggcggctct gacatccaga tgacccagtc tccatcctcc 1800
ctgtctgcat ctgtaggaga cagagtcacc atcacttgcc aggccagtca gagcatttac 1860
agctacttaa actggtatca gcagaaacca gggaaagccc ctaagctcct gatctatggt 1920
gcatccaatc tggcatctgg ggtcccatca aggttcagtg gcagtggatc tgggacagat 1980
ttcactctca ccatcagcag tctgcaacct gaagattttg caacttacta ctgtcaaagc 2040
agttggttga gtggtgctgt tggtaatgct ttcggcggag ggaccaaggt ggagatcaaa 2100
<210> 18
<211> 700
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 18
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Glu Gln Leu Leu Glu Ser
450 455 460
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr
465 470 475 480
Ala Ser Gly Phe Ser Phe Ser Ser Tyr Trp Met Cys Trp Val Arg Gln
485 490 495
Ala Pro Gly Lys Gly Leu Glu Trp Ile Gly Cys Ile Thr Thr Gly Ser
500 505 510
Gly Ser Thr Tyr Tyr Ala Ser Trp Ala Lys Arg Arg Phe Thr Ile Ser
515 520 525
Lys Asp Asn Ser Lys Asn Thr Val Thr Leu Gln Met Asn Ser Leu Arg
530 535 540
Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr Arg Ala Phe Asp Leu Trp
545 550 555 560
Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
565 570 575
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile
580 585 590
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg
595 600 605
Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Tyr Ser Tyr Leu Asn
610 615 620
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Gly
625 630 635 640
Ala Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly
645 650 655
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp
660 665 670
Phe Ala Thr Tyr Tyr Cys Gln Ser Ser Trp Leu Ser Gly Ala Val Gly
675 680 685
Asn Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 19
<211> 2097
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 19
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc acaggagcag 1380
gtgaaggaga ccgggggagg cttggtacag cctggggggt ccctgagact ctcctgtgca 1440
gcctctggat tcaccatcag cagctatgga gtgagctggg tccgccaggc tccagggaag 1500
gggctggagt gggtcgcatt gatttttccc gggattggtt tcaaagacta cgcgagctgg 1560
gtgaatggcc ggttcaccct ctccagcgac aacgcccaga acactgtgga actgcaaatg 1620
aacagcctga gagccgagga cacggccgta tattactgtg cgagagattt ggacttgtgg 1680
ggccagggaa ccctggtcac cgtctcgagc ggtggaggcg gatctggcgg aggtggttcc 1740
ggcggtggcg gctccggtgg aggcggctct gacatccaga tgacccagtc tccatcctcc 1800
ctgtctgcat ctgtaggaga cagagtcacc atcacttgcc agtccagtcc gagtgtttat 1860
agtaactact tatcctggta tcagcagaaa ccagggaaag ttcctaagct cctgatctat 1920
tatgcatcca ctctggcatc tggggtccca tctcggttca gtggcagtgg atctgggaca 1980
gatttcactc tcaccatcag cagcctgcag cctgaagatg ttgcaactta ttactgtgca 2040
ggcggttata gtagtagtac tcgtgctttc ggcggaggga ccaaggtgga gatcaaa 2097
<210> 20
<211> 699
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 20
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Glu Gln Val Lys Glu Thr
450 455 460
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala
465 470 475 480
Ala Ser Gly Phe Thr Ile Ser Ser Tyr Gly Val Ser Trp Val Arg Gln
485 490 495
Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Leu Ile Phe Pro Gly Ile
500 505 510
Gly Phe Lys Asp Tyr Ala Ser Trp Val Asn Gly Arg Phe Thr Leu Ser
515 520 525
Ser Asp Asn Ala Gln Asn Thr Val Glu Leu Gln Met Asn Ser Leu Arg
530 535 540
Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Leu Asp Leu Trp
545 550 555 560
Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
565 570 575
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile
580 585 590
Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg
595 600 605
Val Thr Ile Thr Cys Gln Ser Ser Pro Ser Val Tyr Ser Asn Tyr Leu
610 615 620
Ser Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile Tyr
625 630 635 640
Tyr Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser
645 650 655
Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu
660 665 670
Asp Val Ala Thr Tyr Tyr Cys Ala Gly Gly Tyr Ser Ser Ser Thr Arg
675 680 685
Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
690 695
<210> 21
<211> 2130
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 21
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctcagctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc agaggtgcag 1380
ctgttggagt ctgggggagg cttggtacag cctggggggt ccctgagact ctcctgtgca 1440
gcctctggat tcacctttag cagcagccac tggatatgct gggtccgcca ggctccaggg 1500
aaggggctgg agtggatcgc atgcatttat actggtagta ttgatgtctt ttactacgcg 1560
agctgggcga aaggccggtt caccatctcc agagacaatt ccaagaacac gctgtatctg 1620
caaatgaaca gcctgagagc cgaggacacg gccgtatatt actgtgcgag agccgctaat 1680
actgatacta cctactttaa cttgtggggc cagggaaccc tggtcaccgt ctcgagcggt 1740
ggaggcggat ctggcggagg tggttccggc ggtggcggct ccggtggagg cggctctgcc 1800
tatgatatga cccagtctcc atcctccctg tctgcatctg taggagacag agtcaccatc 1860
aattgccagg ccagtcagag cattaacaac caactatcct ggtatcagca gaaaccaggg 1920
aaagttccta agctcctgat ctatggtgca tccactctgg catctggggt cccatctcgg 1980
ttcaccggca gtggatctgg gacagatttc actctcacca tcagcagcct gcagcctgaa 2040
gatgttgcaa cttattactg tcatgttcat tattgcagtg gtggtagttg tttttgggct 2100
ttcggcggag ggaccaaggt ggagatcaaa 2130
<210> 22
<211> 710
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 22
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Leu Glu Ser
450 455 460
Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala
465 470 475 480
Ala Ser Gly Phe Thr Phe Ser Ser Ser His Trp Ile Cys Trp Val Arg
485 490 495
Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile Ala Cys Ile Tyr Thr Gly
500 505 510
Ser Ile Asp Val Phe Tyr Tyr Ala Ser Trp Ala Lys Gly Arg Phe Thr
515 520 525
Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser
530 535 540
Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ala Ala Asn
545 550 555 560
Thr Asp Thr Thr Tyr Phe Asn Leu Trp Gly Gln Gly Thr Leu Val Thr
565 570 575
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
580 585 590
Gly Ser Gly Gly Gly Gly Ser Ala Tyr Asp Met Thr Gln Ser Pro Ser
595 600 605
Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Asn Cys Gln Ala
610 615 620
Ser Gln Ser Ile Asn Asn Gln Leu Ser Trp Tyr Gln Gln Lys Pro Gly
625 630 635 640
Lys Val Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly
645 650 655
Val Pro Ser Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu
660 665 670
Thr Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys His
675 680 685
Val His Tyr Cys Ser Gly Gly Ser Cys Phe Trp Ala Phe Gly Gly Gly
690 695 700
Thr Lys Val Glu Ile Lys
705 710
<210> 23
<211> 60
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 23
ggtggaggcg gatctggcgg aggtggttcc ggcggtggcg gctccggtgg aggcggctct 60
<210> 24
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 24
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
1 5 10 15
Gly Gly Gly Ser
20
<210> 25
<211> 2100
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 25
cagtcggtgg aggagtctgg gggaggcttg gtccagcctg gggggtccct gagactctcc 60
tgtaccgcct ctggaatcga ccttaatacc tacgacatga tctgggtccg ccaggctcca 120
ggcaaggggc tagagtgggt tggaatcatt acttatagtg gtagtagata ctacgcgaac 180
tgggcgaaag gccgattcac catctccaaa gacaatacca agaacacggt gtatctgcaa 240
atgaacagcc tgagagctga ggacacggct gtgtattact gtgcgagaga ttatatgagt 300
ggttcccact tgtggggcca gggaaccctg gtcaccgtct cgagcgctag caccaagggc 360
ccatcggtct tccccctggc accctcctcc aagagcacct ctgggggcac agcggccctg 420
ggctgcctgg tcaaggacta cttccccgaa ccggtgacgg tgtcgtggaa ctcaggcgcc 480
ctgaccagcg gcgtgcacac cttcccggct gtcctacagt cctcaggact ctactccctc 540
agcagcgtgg tgaccgtgcc ctccagcagc ttgggcaccc agacctacat ctgcaacgtg 600
aatcacaagc ccagcaacac caaggtggac aagagagttg agcccaaatc ttgtgacaaa 660
actcacacat gcccaccgtg cccagcacct gaagccgcgg gggcaccgtc agtcttcctc 720
ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg 780
gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg 840
gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg 900
gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcgcg 960
gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc caaagggcag 1020
ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac caagaaccag 1080
gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag 1140
agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc 1200
tccttcttcc tctatagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc 1260
ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc 1320
ctgtctccgg gtggcggtgg agggtccggc ggtggtggat cccaggtgca attggtggag 1380
tctgggggag gcgtggtcca gcctgggagg tccctgagac tctcctgtgc agcctctgga 1440
ttcaccttca gtagctatac tatgcactgg gtccgccagg ctccaggcaa ggggctggag 1500
tgggtgacat ttatatcata tgatggaaac aataaatact acgcagactc cgtgaagggc 1560
cgattcacca tctccagaga caattccaag aacacgctgt atctgcaaat gaacagcctg 1620
agagctgagg acacggctat atattactgt gcgaggaccg gctggctggg gccctttgac 1680
tactggggcc agggaaccct ggtcaccgtc tcgagcggtg gaggcggttc aggcggaggt 1740
ggaagtggtg gtggcggctc tggaggcggc ggatctgaaa ttgtgttgac gcagtctcca 1800
ggcaccctgt ctttgtctcc aggggaaaga gccaccctct cctgcagggc cagtcagagt 1860
gttggcagca gctacttagc ctggtaccag cagaaacctg gccaggctcc caggctcctc 1920
atctatggtg cattcagcag ggccactggc atcccagaca ggttcagtgg cagtgggtct 1980
gggacagact tcactctcac catcagcaga ctggagcctg aagattttgc agtgtattac 2040
tgtcagcagt atggtagctc accgtggacg ttcggccaag ggaccaaggt ggaaatcaaa 2100
<210> 26
<211> 700
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 26
Gln Ser Val Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser
1 5 10 15
Leu Arg Leu Ser Cys Thr Ala Ser Gly Ile Asp Leu Asn Thr Tyr Asp
20 25 30
Met Ile Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Gly
35 40 45
Ile Ile Thr Tyr Ser Gly Ser Arg Tyr Tyr Ala Asn Trp Ala Lys Gly
50 55 60
Arg Phe Thr Ile Ser Lys Asp Asn Thr Lys Asn Thr Val Tyr Leu Gln
65 70 75 80
Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg
85 90 95
Asp Tyr Met Ser Gly Ser His Leu Trp Gly Gln Gly Thr Leu Val Thr
100 105 110
Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro
115 120 125
Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val
130 135 140
Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala
145 150 155 160
Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly
165 170 175
Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly
180 185 190
Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys
195 200 205
Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys
210 215 220
Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser Val Phe Leu
225 230 235 240
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
245 250 255
Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys
260 265 270
Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
275 280 285
Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu
290 295 300
Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Ala
305 310 315 320
Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys
325 330 335
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
340 345 350
Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
355 360 365
Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln
370 375 380
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
385 390 395 400
Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln
405 410 415
Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
420 425 430
His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly Gly
435 440 445
Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly
450 455 460
Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly
465 470 475 480
Phe Thr Phe Ser Ser Tyr Thr Met His Trp Val Arg Gln Ala Pro Gly
485 490 495
Lys Gly Leu Glu Trp Val Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys
500 505 510
Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn
515 520 525
Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp
530 535 540
Thr Ala Ile Tyr Tyr Cys Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp
545 550 555 560
Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly
565 570 575
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
580 585 590
Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly
595 600 605
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser Ser
610 615 620
Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu
625 630 635 640
Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser
645 650 655
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu
660 665 670
Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro
675 680 685
Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 27
<211> 651
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 27
gcctatgata tgacccagtc tccatcttcc gtgtctgcat ctgtaggaga cagagtcacc 60
atcaagtgtc aggccagtga ggacatttat agcttcttgg cctggtatca gcagaaacca 120
gggaaagccc ctaagctcct gatccattct gcatcctctc tggcatctgg ggtcccatca 180
aggttcagcg gcagtggatc tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagattttg caacttacta ttgtcaacag ggttatggta aaaataatgt tgataatgct 300
ttcggcggag ggaccaaggt ggagatcaaa cgtacggtgg ctgcaccatc tgtcttcatc 360
ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat 420
aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt 480
aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc 540
accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc 600
catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg t 651
<210> 28
<211> 217
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 28
Ala Tyr Asp Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Lys Cys Gln Ala Ser Glu Asp Ile Tyr Ser Phe
20 25 30
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
35 40 45
His Ser Ala Ser Ser Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Tyr Gly Lys Asn Asn
85 90 95
Val Asp Asn Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr
100 105 110
Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu
115 120 125
Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro
130 135 140
Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly
145 150 155 160
Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr
165 170 175
Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His
180 185 190
Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val
195 200 205
Thr Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 29
<211> 2100
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 29
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtacag cctctggatt ctccctcagt agctatgcaa tgagctgggt ccgccaggct 120
ccagggaagg ggctggagta catcggctac attggtgata ctactggcat agcctacgcg 180
agctgggcga atggcagatt caccatctcc aaagacaata ccaagaacac ggtggatctt 240
caaatgaaca gcctgagagc cgaggacacg gctgtgtatt actgtgcgag aggctggtcc 300
tacttagaca tctggggcca agggaccctg gtcaccgtct cgagcgctag caccaagggc 360
ccatcggtct tccccctggc accctcctcc aagagcacct ctgggggcac agcggccctg 420
ggctgcctgg tcaaggacta cttccccgaa ccggtgacgg tgtcgtggaa ctcaggcgcc 480
ctgaccagcg gcgtgcacac cttcccggct gtcctacagt cctcaggact ctactccctc 540
agcagcgtgg tgaccgtgcc ctccagcagc ttgggcaccc agacctacat ctgcaacgtg 600
aatcacaagc ccagcaacac caaggtggac aagagagttg agcccaaatc ttgtgacaaa 660
actcacacat gcccaccgtg cccagcacct gaagccgcgg gggcaccgtc agtcttcctc 720
ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg 780
gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg 840
gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg 900
gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcgcg 960
gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc caaagggcag 1020
ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac caagaaccag 1080
gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag 1140
agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc 1200
tccttcttcc tctatagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc 1260
ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc 1320
ctgtctccgg gtggcggtgg agggtccggc ggtggtggat cccaggtgca attggtggag 1380
tctgggggag gcgtggtcca gcctgggagg tccctgagac tctcctgtgc agcctctgga 1440
ttcaccttca gtagctatac tatgcactgg gtccgccagg ctccaggcaa ggggctggag 1500
tgggtgacat ttatatcata tgatggaaac aataaatact acgcagactc cgtgaagggc 1560
cgattcacca tctccagaga caattccaag aacacgctgt atctgcaaat gaacagcctg 1620
agagctgagg acacggctat atattactgt gcgaggaccg gctggctggg gccctttgac 1680
tactggggcc agggaaccct ggtcaccgtc tcgagcggtg gaggcggatc tggcggaggt 1740
ggttccggcg gtggcggctc cggtggaggc ggttctgaaa ttgtgttgac gcagtctcca 1800
ggcaccctgt ctttgtctcc aggggaaaga gccaccctct cctgcagggc cagtcagagt 1860
gttggcagca gctacttagc ctggtaccag cagaaacctg gccaggctcc caggctcctc 1920
atctatggtg cattcagcag ggccactggc atcccagaca ggttcagtgg cagtgggtct 1980
gggacagact tcactctcac catcagcaga ctggagcctg aagattttgc agtgtattac 2040
tgtcagcagt atggtagctc accgtggacg ttcggccaag ggaccaaggt ggaaatcaaa 2100
<210> 30
<211> 700
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 30
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Ser Ser Tyr
20 25 30
Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Tyr Ile
35 40 45
Gly Tyr Ile Gly Asp Thr Thr Gly Ile Ala Tyr Ala Ser Trp Ala Asn
50 55 60
Gly Arg Phe Thr Ile Ser Lys Asp Asn Thr Lys Asn Thr Val Asp Leu
65 70 75 80
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Arg Gly Trp Ser Tyr Leu Asp Ile Trp Gly Gln Gly Thr Leu Val Thr
100 105 110
Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro
115 120 125
Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val
130 135 140
Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala
145 150 155 160
Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly
165 170 175
Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly
180 185 190
Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys
195 200 205
Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys
210 215 220
Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser Val Phe Leu
225 230 235 240
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
245 250 255
Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys
260 265 270
Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
275 280 285
Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu
290 295 300
Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Ala
305 310 315 320
Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys
325 330 335
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
340 345 350
Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
355 360 365
Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln
370 375 380
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
385 390 395 400
Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln
405 410 415
Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
420 425 430
His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly Gly
435 440 445
Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly
450 455 460
Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly
465 470 475 480
Phe Thr Phe Ser Ser Tyr Thr Met His Trp Val Arg Gln Ala Pro Gly
485 490 495
Lys Gly Leu Glu Trp Val Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys
500 505 510
Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn
515 520 525
Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp
530 535 540
Thr Ala Ile Tyr Tyr Cys Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp
545 550 555 560
Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly
565 570 575
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
580 585 590
Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly
595 600 605
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser Ser
610 615 620
Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu
625 630 635 640
Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser
645 650 655
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu
660 665 670
Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro
675 680 685
Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 31
<211> 648
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 31
gcccttgtga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc aggccagtca gaacatttac agcaatttag cctggtatca gcagaaacca 120
gggaaagttc ctaagctcct gatctatcag gcctccactc tggcatctgg ggtcccatct 180
cggttcagtg gcagtggata tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagatgttg caacttatta ctgtcaaggc ggttattata gtgctgccct taatactttc 300
ggcggaggga ccaaggtgga gatcaaacgt acggtggctg caccatctgt cttcatcttc 360
ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac 420
ttctatccca gagaggccaa agtacagtgg aaggtggata acgccctcca atcgggtaac 480
tcccaggaga gtgtcacaga gcaggacagc aaggacagca cctacagcct cagcagcacc 540
ctgacgctga gcaaagcaga ctacgagaaa cacaaagtct acgcctgcga agtcacccat 600
cagggcctga gctcgcccgt cacaaagagc ttcaacaggg gagagtgt 648
<210> 32
<211> 216
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 32
Ala Leu Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Asn Ile Tyr Ser Asn
20 25 30
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile
35 40 45
Tyr Gln Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Tyr Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gly Gly Tyr Tyr Ser Ala Ala
85 90 95
Leu Asn Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val
100 105 110
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys
115 120 125
Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg
130 135 140
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn
145 150 155 160
Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser
165 170 175
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys
180 185 190
Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr
195 200 205
Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 33
<211> 2121
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 33
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt ctccttcagt agcgggtacg acatgtgctg ggtccgccag 120
gctccaggga aggggctgga gtggatcgca tgcattgctg ctggtagtgc tggtatcact 180
tacgacgcga actgggcgaa aggccggttc accatctcca gagacaattc caagaacacg 240
ctgtatctgc aaatgaacag cctgagagcc gaggacacgg ccgtatatta ctgtgcgaga 300
tcggcgtttt cgttcgacta cgccatggac ctctggggcc agggaaccct ggtcaccgtc 360
tcgagcgcta gcaccaaggg cccatcggtc ttccccctgg caccctcctc caagagcacc 420
tctgggggca cagcggccct gggctgcctg gtcaaggact acttccccga accggtgacg 480
gtgtcgtgga actcaggcgc cctgaccagc ggcgtgcaca ccttcccggc tgtcctacag 540
tcctcaggac tctactccct cagcagcgtg gtgaccgtgc cctccagcag cttgggcacc 600
cagacctaca tctgcaacgt gaatcacaag cccagcaaca ccaaggtgga caagagagtt 660
gagcccaaat cttgtgacaa aactcacaca tgcccaccgt gcccagcacc tgaagccgcg 720
ggggcaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg 780
acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc 840
aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag 900
tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat 960
ggcaaggagt acaagtgcgc ggtctccaac aaagccctcc cagcccccat cgagaaaacc 1020
atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg 1080
gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc 1140
gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct 1200
cccgtgctgg actccgacgg ctccttcttc ctctatagca agctcaccgt ggacaagagc 1260
aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac 1320
tacacgcaga agagcctctc cctgtctccg ggtggcggtg gagggtccgg cggtggtgga 1380
tcccaggtgc aattggtgga gtctggggga ggcgtggtcc agcctgggag gtccctgaga 1440
ctctcctgtg cagcctctgg attcaccttc agtagctata ctatgcactg ggtccgccag 1500
gctccaggca aggggctgga gtgggtgaca tttatatcat atgatggaaa caataaatac 1560
tacgcagact ccgtgaaggg ccgattcacc atctccagag acaattccaa gaacacgctg 1620
tatctgcaaa tgaacagcct gagagctgag gacacggcta tatattactg tgcgaggacc 1680
ggctggctgg ggccctttga ctactggggc cagggaaccc tggtcaccgt ctcgagcggt 1740
ggaggcggat ctggcggagg tggttccggc ggtggcggct ccggtggagg cggttctgaa 1800
attgtgttga cgcagtctcc aggcaccctg tctttgtctc caggggaaag agccaccctc 1860
tcctgcaggg ccagtcagag tgttggcagc agctacttag cctggtacca gcagaaacct 1920
ggccaggctc ccaggctcct catctatggt gcattcagca gggccactgg catcccagac 1980
aggttcagtg gcagtgggtc tgggacagac ttcactctca ccatcagcag actggagcct 2040
gaagattttg cagtgtatta ctgtcagcag tatggtagct caccgtggac gttcggccaa 2100
gggaccaagg tggaaatcaa a 2121
<210> 34
<211> 707
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 34
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Phe Ser Ser Gly
20 25 30
Tyr Asp Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp
35 40 45
Ile Ala Cys Ile Ala Ala Gly Ser Ala Gly Ile Thr Tyr Asp Ala Asn
50 55 60
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr
65 70 75 80
Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr
85 90 95
Tyr Cys Ala Arg Ser Ala Phe Ser Phe Asp Tyr Ala Met Asp Leu Trp
100 105 110
Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro
115 120 125
Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr
130 135 140
Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr
145 150 155 160
Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro
165 170 175
Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr
180 185 190
Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn
195 200 205
His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser
210 215 220
Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala
225 230 235 240
Gly Ala Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
245 250 255
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
260 265 270
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
275 280 285
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr
290 295 300
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
305 310 315 320
Gly Lys Glu Tyr Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro
325 330 335
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
340 345 350
Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val
355 360 365
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
370 375 380
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
385 390 395 400
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
405 410 415
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
420 425 430
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
435 440 445
Ser Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln
450 455 460
Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg
465 470 475 480
Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Thr Met His
485 490 495
Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Thr Phe Ile
500 505 510
Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg
515 520 525
Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met
530 535 540
Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Thr
545 550 555 560
Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr
565 570 575
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
580 585 590
Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly
595 600 605
Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala
610 615 620
Ser Gln Ser Val Gly Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro
625 630 635 640
Gly Gln Ala Pro Arg Leu Leu Ile Tyr Gly Ala Phe Ser Arg Ala Thr
645 650 655
Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr
660 665 670
Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys
675 680 685
Gln Gln Tyr Gly Ser Ser Pro Trp Thr Phe Gly Gln Gly Thr Lys Val
690 695 700
Glu Ile Lys
705
<210> 35
<211> 651
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 35
gacatccaga tgacccagtc tccttccacc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc aggccagtca gagcattagt tcccacttaa actggtatca gcagaaacca 120
gggaaagccc ctaagctcct gatctataag gcatccactc tggcatctgg ggtcccatca 180
aggttcagcg gcagtggatc tgggacagaa tttactctca ccatcagcag cctgcagcct 240
gatgattttg caacttatta ctgccaacag ggttatagtt ggggtaatgt tgataatgtt 300
ttcggcggag ggaccaaggt ggagatcaaa cgtacggtgg ctgcaccatc tgtcttcatc 360
ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat 420
aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt 480
aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc 540
accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc 600
catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg t 651
<210> 36
<211> 217
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 36
Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Ser Ser His
20 25 30
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
35 40 45
Tyr Lys Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Tyr Ser Trp Gly Asn
85 90 95
Val Asp Asn Val Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr
100 105 110
Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu
115 120 125
Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro
130 135 140
Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly
145 150 155 160
Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr
165 170 175
Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His
180 185 190
Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val
195 200 205
Thr Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 37
<211> 2103
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 37
caggtgcagc tggtggagtc tgggggaggc ctggtccagc ctgggggctc cctgagactc 60
tcctgtacaa cttctggaat cgaccttagt acctacgaca tgatctgggt ccgccaggct 120
ccaggcaagg ggctagagtg ggtgggaatc attagttatg ttggtaacac atactacgcg 180
agctgggcga aaggccgatt caccctctcc aaagacaata cctcgaccac ggtggatctg 240
caaatgaaca gcctgagagc tgaggacacg gctgtgtatt actgtgcgag agattttatt 300
agtggttccc acttgtgggg ccagggaacc ctggtcaccg tctcgagcgc tagcaccaag 360
ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc 420
ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc 480
gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc 540
ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac 600
gtgaatcaca agcccagcaa caccaaggtg gacaagagag ttgagcccaa atcttgtgac 660
aaaactcaca catgcccacc gtgcccagca cctgaagccg cgggggcacc gtcagtcttc 720
ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc 780
gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc 840
gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt 900
gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc 960
gcggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg 1020
cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac 1080
caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg 1140
gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac 1200
ggctccttct tcctctatag caagctcacc gtggacaaga gcaggtggca gcaggggaac 1260
gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc 1320
tccctgtctc cgggtggcgg tggagggtcc ggcggtggtg gatcccaggt gcaattggtg 1380
gagtctgggg gaggcgtggt ccagcctggg aggtccctga gactctcctg tgcagcctct 1440
ggattcacct tcagtagcta tactatgcac tgggtccgcc aggctccagg caaggggctg 1500
gagtgggtga catttatatc atatgatgga aacaataaat actacgcaga ctccgtgaag 1560
ggccgattca ccatctccag agacaattcc aagaacacgc tgtatctgca aatgaacagc 1620
ctgagagctg aggacacggc tatatattac tgtgcgagga ccggctggct ggggcccttt 1680
gactactggg gccagggaac cctggtcacc gtctcgagcg gtggaggcgg atctggcgga 1740
ggtggttccg gcggtggcgg ctccggtgga ggcggttctg aaattgtgtt gacgcagtct 1800
ccaggcaccc tgtctttgtc tccaggggaa agagccaccc tctcctgcag ggccagtcag 1860
agtgttggca gcagctactt agcctggtac cagcagaaac ctggccaggc tcccaggctc 1920
ctcatctatg gtgcattcag cagggccact ggcatcccag acaggttcag tggcagtggg 1980
tctgggacag acttcactct caccatcagc agactggagc ctgaagattt tgcagtgtat 2040
tactgtcagc agtatggtag ctcaccgtgg acgttcggcc aagggaccaa ggtggaaatc 2100
aaa 2103
<210> 38
<211> 701
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 38
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Thr Ser Gly Ile Asp Leu Ser Thr Tyr
20 25 30
Asp Met Ile Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Gly Ile Ile Ser Tyr Val Gly Asn Thr Tyr Tyr Ala Ser Trp Ala Lys
50 55 60
Gly Arg Phe Thr Leu Ser Lys Asp Asn Thr Ser Thr Thr Val Asp Leu
65 70 75 80
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Arg Asp Phe Ile Ser Gly Ser His Leu Trp Gly Gln Gly Thr Leu Val
100 105 110
Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
115 120 125
Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
130 135 140
Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145 150 155 160
Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
165 170 175
Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
180 185 190
Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
195 200 205
Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
210 215 220
Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser Val Phe
225 230 235 240
Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
245 250 255
Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
260 265 270
Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
275 280 285
Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
290 295 300
Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305 310 315 320
Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
325 330 335
Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
340 345 350
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
355 360 365
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
370 375 380
Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385 390 395 400
Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
405 410 415
Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
420 425 430
Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly
435 440 445
Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly
450 455 460
Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser
465 470 475 480
Gly Phe Thr Phe Ser Ser Tyr Thr Met His Trp Val Arg Gln Ala Pro
485 490 495
Gly Lys Gly Leu Glu Trp Val Thr Phe Ile Ser Tyr Asp Gly Asn Asn
500 505 510
Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp
515 520 525
Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu
530 535 540
Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Thr Gly Trp Leu Gly Pro Phe
545 550 555 560
Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly
565 570 575
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
580 585 590
Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro
595 600 605
Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser
610 615 620
Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu
625 630 635 640
Leu Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe
645 650 655
Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu
660 665 670
Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser
675 680 685
Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 39
<211> 651
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 39
gcctatgata tgacccagtc tccatcttcc gtgtctgcat ctgtaggaga cagagtcacc 60
atcaattgtc aggccagtga gagcattagt agcttcttat cctggtatca gcagaaacca 120
gggaaagccc ctaagctcct gatcttttct gcatccactc tggcatctgg ggtcccatca 180
aggttcagcg gcagtggatc tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagattttg caacttacta ttgtcaacag ggttatagta aaagtaatgt ggataatgct 300
ttcggcggag ggaccaaggt ggagatcaaa cgtacggtgg ctgcaccatc tgtcttcatc 360
ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat 420
aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt 480
aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc 540
accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc 600
catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg t 651
<210> 40
<211> 217
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 40
Ala Tyr Asp Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Glu Ser Ile Ser Ser Phe
20 25 30
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
35 40 45
Phe Ser Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Tyr Ser Lys Ser Asn
85 90 95
Val Asp Asn Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr
100 105 110
Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu
115 120 125
Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro
130 135 140
Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly
145 150 155 160
Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr
165 170 175
Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His
180 185 190
Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val
195 200 205
Thr Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 41
<211> 2112
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 41
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt ctccctcagt agctactgga tgagctgggt ccgccaggct 120
ccagggaagg ggctggagtg gatcggagtc attgatacta atgtttatat atactacgcg 180
aactgggcaa aaggcagatt caccatctcc agagacaatt ccaagaacac gctgtatctt 240
caaatgaaca gcctgagagc cgaggacacg gctgtgtatt actgtgcgag atatgtgggt 300
aataatgatg attatattaa cttgtggggc cagggaaccc tggtcaccgt ctcgagcgct 360
agcaccaagg gcccatcggt cttccccctg gcaccctcct ccaagagcac ctctgggggc 420
acagcggccc tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg 480
aactcaggcg ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga 540
ctctactccc tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac 600
atctgcaacg tgaatcacaa gcccagcaac accaaggtgg acaagagagt tgagcccaaa 660
tcttgtgaca aaactcacac atgcccaccg tgcccagcac ctgaagccgc gggggcaccg 720
tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 780
gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac 840
gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc 900
acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag 960
tacaagtgcg cggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa 1020
gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggatgagctg 1080
accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc 1140
gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg 1200
gactccgacg gctccttctt cctctatagc aagctcaccg tggacaagag caggtggcag 1260
caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1320
aagagcctct ccctgtctcc gggtggcggt ggagggtccg gcggtggtgg atcccaggtg 1380
caattggtgg agtctggggg aggcgtggtc cagcctggga ggtccctgag actctcctgt 1440
gcagcctctg gattcacctt cagtagctat actatgcact gggtccgcca ggctccaggc 1500
aaggggctgg agtgggtgac atttatatca tatgatggaa acaataaata ctacgcagac 1560
tccgtgaagg gccgattcac catctccaga gacaattcca agaacacgct gtatctgcaa 1620
atgaacagcc tgagagctga ggacacggct atatattact gtgcgaggac cggctggctg 1680
gggccctttg actactgggg ccagggaacc ctggtcaccg tctcgagcgg tggaggcgga 1740
tctggcggag gtggttccgg cggtggcggc tccggtggag gcggttctga aattgtgttg 1800
acgcagtctc caggcaccct gtctttgtct ccaggggaaa gagccaccct ctcctgcagg 1860
gccagtcaga gtgttggcag cagctactta gcctggtacc agcagaaacc tggccaggct 1920
cccaggctcc tcatctatgg tgcattcagc agggccactg gcatcccaga caggttcagt 1980
ggcagtgggt ctgggacaga cttcactctc accatcagca gactggagcc tgaagatttt 2040
gcagtgtatt actgtcagca gtatggtagc tcaccgtgga cgttcggcca agggaccaag 2100
gtggaaatca aa 2112
<210> 42
<211> 704
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 42
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser Tyr
20 25 30
Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
35 40 45
Gly Val Ile Asp Thr Asn Val Tyr Ile Tyr Tyr Ala Asn Trp Ala Lys
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Arg Tyr Val Gly Asn Asn Asp Asp Tyr Ile Asn Leu Trp Gly Gln Gly
100 105 110
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe
115 120 125
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu
130 135 140
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145 150 155 160
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
165 170 175
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
180 185 190
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
195 200 205
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys
210 215 220
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro
225 230 235 240
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
245 250 255
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
260 265 270
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
275 280 285
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
290 295 300
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
305 310 315 320
Tyr Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
325 330 335
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
340 345 350
Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr
355 360 365
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
370 375 380
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
385 390 395 400
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
405 410 415
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
420 425 430
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
435 440 445
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Glu
450 455 460
Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys
465 470 475 480
Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Thr Met His Trp Val Arg
485 490 495
Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Thr Phe Ile Ser Tyr Asp
500 505 510
Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile
515 520 525
Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu
530 535 540
Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Thr Gly Trp Leu
545 550 555 560
Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
565 570 575
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
580 585 590
Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser
595 600 605
Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser
610 615 620
Val Gly Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala
625 630 635 640
Pro Arg Leu Leu Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro
645 650 655
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile
660 665 670
Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr
675 680 685
Gly Ser Ser Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 43
<211> 654
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 43
gacatccaga tgacccagtc tccttccacc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc agtccagtca gagtgtttat aatggctact ggttatcctg gtatcagcag 120
aaaccaggga aagcccctaa gctcctgatc tatggtgcat ccactctggc atctggggtc 180
ccatcaaggt tcagcggcag tggatctggg acagaattca ctctcaccat cagcagcctg 240
cagcctgatg attttgcaac ttattactgc ctaggcagtt atactagtag tactgagaac 300
tctttcggcg gagggaccaa ggtggagatc aaacgtacgg tggctgcacc atctgtcttc 360
atcttcccgc catctgatga gcagttgaaa tctggaactg cctctgttgt gtgcctgctg 420
aataacttct atcccagaga ggccaaagta cagtggaagg tggataacgc cctccaatcg 480
ggtaactccc aggagagtgt cacagagcag gacagcaagg acagcaccta cagcctcagc 540
agcaccctga cgctgagcaa agcagactac gagaaacaca aagtctacgc ctgcgaagtc 600
acccatcagg gcctgagctc gcccgtcaca aagagcttca acaggggaga gtgt 654
<210> 44
<211> 218
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 44
Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Gln Ser Val Tyr Asn Gly
20 25 30
Tyr Trp Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu
35 40 45
Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe
50 55 60
Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu
65 70 75 80
Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Leu Gly Ser Tyr Thr Ser
85 90 95
Ser Thr Glu Asn Ser Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg
100 105 110
Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln
115 120 125
Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr
130 135 140
Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser
145 150 155 160
Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr
165 170 175
Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys
180 185 190
His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro
195 200 205
Val Thr Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 45
<211> 2109
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 45
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtacag tgtctggaat cgacctcagt gtcatcaata tgggctgggt ccgccaggct 120
ccagggaagg ggctggagtg gatcggaacc attacttatg ttggtaacac atattacgcg 180
agctgggcga aaggcagatt caccatctcc aaaacctcga ccacggtgga tcttaaaatc 240
accagtccga caaccgagga cacggctgtg tattactgtg cgagagaatc tggtactatt 300
tattacagtt actttaactt gtggggccaa gggaccctgg tcaccgtctc gagcgctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc ccaggtgcaa 1380
ttggtggagt ctgggggagg cgtggtccag cctgggaggt ccctgagact ctcctgtgca 1440
gcctctggat tcaccttcag tagctatact atgcactggg tccgccaggc tccaggcaag 1500
gggctggagt gggtgacatt tatatcatat gatggaaaca ataaatacta cgcagactcc 1560
gtgaagggcc gattcaccat ctccagagac aattccaaga acacgctgta tctgcaaatg 1620
aacagcctga gagctgagga cacggctata tattactgtg cgaggaccgg ctggctgggg 1680
ccctttgact actggggcca gggaaccctg gtcaccgtct cgagcggtgg aggcggatct 1740
ggcggaggtg gttccggcgg tggcggctcc ggtggaggcg gttctgaaat tgtgttgacg 1800
cagtctccag gcaccctgtc tttgtctcca ggggaaagag ccaccctctc ctgcagggcc 1860
agtcagagtg ttggcagcag ctacttagcc tggtaccagc agaaacctgg ccaggctccc 1920
aggctcctca tctatggtgc attcagcagg gccactggca tcccagacag gttcagtggc 1980
agtgggtctg ggacagactt cactctcacc atcagcagac tggagcctga agattttgca 2040
gtgtattact gtcagcagta tggtagctca ccgtggacgt tcggccaagg gaccaaggtg 2100
gaaatcaaa 2109
<210> 46
<211> 703
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 46
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Val Ser Gly Ile Asp Leu Ser Val Ile
20 25 30
Asn Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
35 40 45
Gly Thr Ile Thr Tyr Val Gly Asn Thr Tyr Tyr Ala Ser Trp Ala Lys
50 55 60
Gly Arg Phe Thr Ile Ser Lys Thr Ser Thr Thr Val Asp Leu Lys Ile
65 70 75 80
Thr Ser Pro Thr Thr Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu
85 90 95
Ser Gly Thr Ile Tyr Tyr Ser Tyr Phe Asn Leu Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser
450 455 460
Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala
465 470 475 480
Ala Ser Gly Phe Thr Phe Ser Ser Tyr Thr Met His Trp Val Arg Gln
485 490 495
Ala Pro Gly Lys Gly Leu Glu Trp Val Thr Phe Ile Ser Tyr Asp Gly
500 505 510
Asn Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser
515 520 525
Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg
530 535 540
Ala Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Thr Gly Trp Leu Gly
545 550 555 560
Pro Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly
565 570 575
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
580 585 590
Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu
595 600 605
Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val
610 615 620
Gly Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro
625 630 635 640
Arg Leu Leu Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp
645 650 655
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser
660 665 670
Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly
675 680 685
Ser Ser Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 47
<211> 648
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 47
gcattcgaat tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcaagtgcc aggccagtga aagcattagc aactacttat cctggtatca gcagattcca 120
gggaaagttc ctaagctcct gatctattat gcatccaatc tggcatctgg ggtcccatct 180
cggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagatgttg caacttatta ctgtcaaagc tattatggtg gtggtagtgc ctatactttc 300
ggcggaggga ccaaggtgga gatcaaacgt acggtggctg caccatctgt cttcatcttc 360
ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac 420
ttctatccca gagaggccaa agtacagtgg aaggtggata acgccctcca atcgggtaac 480
tcccaggaga gtgtcacaga gcaggacagc aaggacagca cctacagcct cagcagcacc 540
ctgacgctga gcaaagcaga ctacgagaaa cacaaagtct acgcctgcga agtcacccat 600
cagggcctga gctcgcccgt cacaaagagc ttcaacaggg gagagtgt 648
<210> 48
<211> 216
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 48
Ala Phe Glu Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Lys Cys Gln Ala Ser Glu Ser Ile Ser Asn Tyr
20 25 30
Leu Ser Trp Tyr Gln Gln Ile Pro Gly Lys Val Pro Lys Leu Leu Ile
35 40 45
Tyr Tyr Ala Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Ser Tyr Tyr Gly Gly Gly Ser
85 90 95
Ala Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val
100 105 110
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys
115 120 125
Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg
130 135 140
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn
145 150 155 160
Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser
165 170 175
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys
180 185 190
Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr
195 200 205
Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 49
<211> 2109
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 49
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt cgacttcagt agcggctact ggatatgctg ggtccgccag 120
gctccaggga aggggctgga gttgatcgca tgcatttatg ctggtactag tggtagtact 180
tcctacgcga gctgggcgag aggcagattc accatctccg aaacctccaa gaacacggtg 240
actcttcaaa tgaacagcct gagagccgag gactcggctg tgtattactg tgcgagaaat 300
ctttacactt acaatagctt gtggggccag ggaaccctgg tcaccgtctc gagcgctagc 360
accaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 420
gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 480
tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 540
tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 600
tgcaacgtga atcacaagcc cagcaacacc aaggtggaca agagagttga gcccaaatct 660
tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg aagccgcggg ggcaccgtca 720
gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc 780
acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg 840
gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg 900
taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac 960
aagtgcgcgg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc 1020
aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc 1080
aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg 1140
gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac 1200
tccgacggct ccttcttcct ctatagcaag ctcaccgtgg acaagagcag gtggcagcag 1260
gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag 1320
agcctctccc tgtctccggg tggcggtgga gggtccggcg gtggtggatc ccaggtgcaa 1380
ttggtggagt ctgggggagg cgtggtccag cctgggaggt ccctgagact ctcctgtgca 1440
gcctctggat tcaccttcag tagctatact atgcactggg tccgccaggc tccaggcaag 1500
gggctggagt gggtgacatt tatatcatat gatggaaaca ataaatacta cgcagactcc 1560
gtgaagggcc gattcaccat ctccagagac aattccaaga acacgctgta tctgcaaatg 1620
aacagcctga gagctgagga cacggctata tattactgtg cgaggaccgg ctggctgggg 1680
ccctttgact actggggcca gggaaccctg gtcaccgtct cgagcggtgg aggcggatct 1740
ggcggaggtg gttccggcgg tggcggctcc ggtggaggcg gttctgaaat tgtgttgacg 1800
cagtctccag gcaccctgtc tttgtctcca ggggaaagag ccaccctctc ctgcagggcc 1860
agtcagagtg ttggcagcag ctacttagcc tggtaccagc agaaacctgg ccaggctccc 1920
aggctcctca tctatggtgc attcagcagg gccactggca tcccagacag gttcagtggc 1980
agtgggtctg ggacagactt cactctcacc atcagcagac tggagcctga agattttgca 2040
gtgtattact gtcagcagta tggtagctca ccgtggacgt tcggccaagg gaccaaggtg 2100
gaaatcaaa 2109
<210> 50
<211> 703
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 50
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Ser Gly
20 25 30
Tyr Trp Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Leu
35 40 45
Ile Ala Cys Ile Tyr Ala Gly Thr Ser Gly Ser Thr Ser Tyr Ala Ser
50 55 60
Trp Ala Arg Gly Arg Phe Thr Ile Ser Glu Thr Ser Lys Asn Thr Val
65 70 75 80
Thr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Ser Ala Val Tyr Tyr
85 90 95
Cys Ala Arg Asn Leu Tyr Thr Tyr Asn Ser Leu Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro
115 120 125
Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly
130 135 140
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn
145 150 155 160
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln
165 170 175
Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser
180 185 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser
195 200 205
Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr
210 215 220
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser
225 230 235 240
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
245 250 255
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
260 265 270
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
275 280 285
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
290 295 300
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
305 310 315 320
Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
325 330 335
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
340 345 350
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
355 360 365
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
370 375 380
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
385 390 395 400
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
405 410 415
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
420 425 430
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly
435 440 445
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser
450 455 460
Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala
465 470 475 480
Ala Ser Gly Phe Thr Phe Ser Ser Tyr Thr Met His Trp Val Arg Gln
485 490 495
Ala Pro Gly Lys Gly Leu Glu Trp Val Thr Phe Ile Ser Tyr Asp Gly
500 505 510
Asn Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser
515 520 525
Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg
530 535 540
Ala Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Thr Gly Trp Leu Gly
545 550 555 560
Pro Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly
565 570 575
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
580 585 590
Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu
595 600 605
Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val
610 615 620
Gly Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro
625 630 635 640
Arg Leu Leu Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp
645 650 655
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser
660 665 670
Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly
675 680 685
Ser Ser Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
690 695 700
<210> 51
<211> 657
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 51
gacatccaga tgacccagtc tccttccacc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc agtccagtca gagtgtttat gataacaact ggttagcctg gtatcagcag 120
aaaccaggga aagcccctaa gctcctgatc tatacagtat ccactctggc atctggggtc 180
ccatcaaggt tcagcggcag tggatctggg acagaattca ctctcaccat cagcagcctg 240
cagcctgatg attttgcaac ttattactgc caaggcactt attatagtag tggttggaac 300
tttgctttcg gcggagggac caaggtggag atcaaacgta cggtggctgc accatctgtc 360
ttcatcttcc cgccatctga tgagcagttg aaatctggaa ctgcctctgt tgtgtgcctg 420
ctgaataact tctatcccag agaggccaaa gtacagtgga aggtggataa cgccctccaa 480
tcgggtaact cccaggagag tgtcacagag caggacagca aggacagcac ctacagcctc 540
agcagcaccc tgacgctgag caaagcagac tacgagaaac acaaagtcta cgcctgcgaa 600
gtcacccatc agggcctgag ctcgcccgtc acaaagagct tcaacagggg agagtgt 657
<210> 52
<211> 219
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 52
Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Gln Ser Val Tyr Asp Asn
20 25 30
Asn Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu
35 40 45
Leu Ile Tyr Thr Val Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe
50 55 60
Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu
65 70 75 80
Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gly Thr Tyr Tyr Ser
85 90 95
Ser Gly Trp Asn Phe Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105 110
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu
115 120 125
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe
130 135 140
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln
145 150 155 160
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser
165 170 175
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu
180 185 190
Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser
195 200 205
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 53
<211> 2094
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 53
caggagcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtacag cctctggatt ctcctttagc agctactgga tgtgctgggt ccgccaggct 120
ccagggaagg ggctggagtg gatcggatgc attacgactg gtagtggtag cacttactac 180
gcgagctggg cgaagcgccg gttcaccatc tccaaagaca attccaagaa cacggtgact 240
ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtac gagagcattt 300
gacttgtggg gccagggaac cctggtcacc gtctcgagcg ctagcaccaa gggcccatcg 360
gtcttccccc tggcaccctc ctccaagagc acctctgggg gcacagcggc cctgggctgc 420
ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc 480
agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagcagc 540
gtggtgaccg tgccctccag cagcttgggc acccagacct acatctgcaa cgtgaatcac 600
aagcccagca acaccaaggt ggacaagaga gttgagccca aatcttgtga caaaactcac 660
acatgcccac cgtgcccagc acctgaagcc gcgggggcac cgtcagtctt cctcttcccc 720
ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 780
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 840
cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 900
gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg cgcggtctcc 960
aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga 1020
gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1080
ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1140
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1200
ttcctctata gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1260
tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1320
ccgggtggcg gtggagggtc cggcggtggt ggatcccagg tgcaattggt ggagtctggg 1380
ggaggcgtgg tccagcctgg gaggtccctg agactctcct gtgcagcctc tggattcacc 1440
ttcagtagct atactatgca ctgggtccgc caggctccag gcaaggggct ggagtgggtg 1500
acatttatat catatgatgg aaacaataaa tactacgcag actccgtgaa gggccgattc 1560
accatctcca gagacaattc caagaacacg ctgtatctgc aaatgaacag cctgagagct 1620
gaggacacgg ctatatatta ctgtgcgagg accggctggc tggggccctt tgactactgg 1680
ggccagggaa ccctggtcac cgtctcgagc ggtggaggcg gatctggcgg aggtggttcc 1740
ggcggtggcg gctccggtgg aggcggttct gaaattgtgt tgacgcagtc tccaggcacc 1800
ctgtctttgt ctccagggga aagagccacc ctctcctgca gggccagtca gagtgttggc 1860
agcagctact tagcctggta ccagcagaaa cctggccagg ctcccaggct cctcatctat 1920
ggtgcattca gcagggccac tggcatccca gacaggttca gtggcagtgg gtctgggaca 1980
gacttcactc tcaccatcag cagactggag cctgaagatt ttgcagtgta ttactgtcag 2040
cagtatggta gctcaccgtg gacgttcggc caagggacca aggtggaaat caaa 2094
<210> 54
<211> 698
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 54
Gln Glu Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Phe Ser Ser Tyr
20 25 30
Trp Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
35 40 45
Gly Cys Ile Thr Thr Gly Ser Gly Ser Thr Tyr Tyr Ala Ser Trp Ala
50 55 60
Lys Arg Arg Phe Thr Ile Ser Lys Asp Asn Ser Lys Asn Thr Val Thr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Thr Arg Ala Phe Asp Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser
100 105 110
Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser
115 120 125
Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp
130 135 140
Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr
145 150 155 160
Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr
165 170 175
Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln
180 185 190
Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp
195 200 205
Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro
210 215 220
Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser Val Phe Leu Phe Pro
225 230 235 240
Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
245 250 255
Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn
260 265 270
Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
275 280 285
Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
290 295 300
Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Ala Val Ser
305 310 315 320
Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys
325 330 335
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp
340 345 350
Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
355 360 365
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
370 375 380
Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385 390 395 400
Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly
405 410 415
Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
420 425 430
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly Gly Ser Gly
435 440 445
Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val
450 455 460
Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr
465 470 475 480
Phe Ser Ser Tyr Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly
485 490 495
Leu Glu Trp Val Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr
500 505 510
Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys
515 520 525
Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala
530 535 540
Ile Tyr Tyr Cys Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp
545 550 555 560
Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
565 570 575
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile
580 585 590
Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg
595 600 605
Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser Ser Tyr Leu
610 615 620
Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr
625 630 635 640
Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly Ser
645 650 655
Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro Glu
660 665 670
Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro Trp Thr
675 680 685
Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
690 695
<210> 55
<211> 651
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 55
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc aggccagtca gagcatttac agctacttaa actggtatca gcagaaacca 120
gggaaagccc ctaagctcct gatctatggt gcatccaatc tggcatctgg ggtcccatca 180
aggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 240
gaagattttg caacttacta ctgtcaaagc agttggttga gtggtgctgt tggtaatgct 300
ttcggcggag ggaccaaggt ggagatcaaa cgtacggtgg ctgcaccatc tgtcttcatc 360
ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat 420
aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt 480
aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc 540
accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc 600
catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg t 651
<210> 56
<211> 217
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 56
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Tyr Ser Tyr
20 25 30
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
35 40 45
Tyr Gly Ala Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Ser Ser Trp Leu Ser Gly Ala
85 90 95
Val Gly Asn Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr
100 105 110
Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu
115 120 125
Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro
130 135 140
Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly
145 150 155 160
Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr
165 170 175
Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His
180 185 190
Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val
195 200 205
Thr Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 57
<211> 2094
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 57
caggagcagg tgaaggagac cgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt caccatcagc agctatggag tgagctgggt ccgccaggct 120
ccagggaagg ggctggagtg ggtcgcattg atttttcccg ggattggttt caaagactac 180
gcgagctggg tgaatggccg gttcaccctc tccagcgaca acgcccagaa cactgtggaa 240
ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gagagatttg 300
gacttgtggg gccagggaac cctggtcacc gtctcgagcg ctagcaccaa gggcccatcg 360
gtcttccccc tggcaccctc ctccaagagc acctctgggg gcacagcggc cctgggctgc 420
ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc 480
agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagcagc 540
gtggtgaccg tgccctccag cagcttgggc acccagacct acatctgcaa cgtgaatcac 600
aagcccagca acaccaaggt ggacaagaga gttgagccca aatcttgtga caaaactcac 660
acatgcccac cgtgcccagc acctgaagcc gcgggggcac cgtcagtctt cctcttcccc 720
ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 780
gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 840
cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 900
gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg cgcggtctcc 960
aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga 1020
gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1080
ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1140
gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1200
ttcctctata gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1260
tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1320
ccgggtggcg gtggagggtc cggcggtggt ggatcccagg tgcaattggt ggagtctggg 1380
ggaggcgtgg tccagcctgg gaggtccctg agactctcct gtgcagcctc tggattcacc 1440
ttcagtagct atactatgca ctgggtccgc caggctccag gcaaggggct ggagtgggtg 1500
acatttatat catatgatgg aaacaataaa tactacgcag actccgtgaa gggccgattc 1560
accatctcca gagacaattc caagaacacg ctgtatctgc aaatgaacag cctgagagct 1620
gaggacacgg ctatatatta ctgtgcgagg accggctggc tggggccctt tgactactgg 1680
ggccagggaa ccctggtcac cgtctcgagc ggtggaggcg gatctggcgg aggtggttcc 1740
ggcggtggcg gctccggtgg aggcggttct gaaattgtgt tgacgcagtc tccaggcacc 1800
ctgtctttgt ctccagggga aagagccacc ctctcctgca gggccagtca gagtgttggc 1860
agcagctact tagcctggta ccagcagaaa cctggccagg ctcccaggct cctcatctat 1920
ggtgcattca gcagggccac tggcatccca gacaggttca gtggcagtgg gtctgggaca 1980
gacttcactc tcaccatcag cagactggag cctgaagatt ttgcagtgta ttactgtcag 2040
cagtatggta gctcaccgtg gacgttcggc caagggacca aggtggaaat caaa 2094
<210> 58
<211> 698
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 58
Gln Glu Gln Val Lys Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Ile Ser Ser Tyr
20 25 30
Gly Val Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Ala Leu Ile Phe Pro Gly Ile Gly Phe Lys Asp Tyr Ala Ser Trp Val
50 55 60
Asn Gly Arg Phe Thr Leu Ser Ser Asp Asn Ala Gln Asn Thr Val Glu
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Arg Asp Leu Asp Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser
100 105 110
Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser
115 120 125
Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp
130 135 140
Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr
145 150 155 160
Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr
165 170 175
Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln
180 185 190
Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp
195 200 205
Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro
210 215 220
Cys Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser Val Phe Leu Phe Pro
225 230 235 240
Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
245 250 255
Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn
260 265 270
Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
275 280 285
Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
290 295 300
Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Ala Val Ser
305 310 315 320
Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys
325 330 335
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp
340 345 350
Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
355 360 365
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
370 375 380
Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385 390 395 400
Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly
405 410 415
Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
420 425 430
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Gly Gly Ser Gly
435 440 445
Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val
450 455 460
Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr
465 470 475 480
Phe Ser Ser Tyr Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly
485 490 495
Leu Glu Trp Val Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr
500 505 510
Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys
515 520 525
Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala
530 535 540
Ile Tyr Tyr Cys Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp
545 550 555 560
Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
565 570 575
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile
580 585 590
Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg
595 600 605
Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser Ser Tyr Leu
610 615 620
Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr
625 630 635 640
Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly Ser
645 650 655
Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro Glu
660 665 670
Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro Trp Thr
675 680 685
Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
690 695
<210> 59
<211> 648
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 59
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc agtccagtcc gagtgtttat agtaactact tatcctggta tcagcagaaa 120
ccagggaaag ttcctaagct cctgatctat tatgcatcca ctctggcatc tggggtccca 180
tctcggttca gtggcagtgg atctgggaca gatttcactc tcaccatcag cagcctgcag 240
cctgaagatg ttgcaactta ttactgtgca ggcggttata gtagtagtac tcgtgctttc 300
ggcggaggga ccaaggtgga gatcaaacgt acggtggctg caccatctgt cttcatcttc 360
ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac 420
ttctatccca gagaggccaa agtacagtgg aaggtggata acgccctcca atcgggtaac 480
tcccaggaga gtgtcacaga gcaggacagc aaggacagca cctacagcct cagcagcacc 540
ctgacgctga gcaaagcaga ctacgagaaa cacaaagtct acgcctgcga agtcacccat 600
cagggcctga gctcgcccgt cacaaagagc ttcaacaggg gagagtgt 648
<210> 60
<211> 216
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 60
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Pro Ser Val Tyr Ser Asn
20 25 30
Tyr Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu
35 40 45
Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser
50 55 60
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
65 70 75 80
Pro Glu Asp Val Ala Thr Tyr Tyr Cys Ala Gly Gly Tyr Ser Ser Ser
85 90 95
Thr Arg Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val
100 105 110
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys
115 120 125
Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg
130 135 140
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn
145 150 155 160
Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser
165 170 175
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys
180 185 190
Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr
195 200 205
Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 61
<211> 2121
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 61
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt cacctttagc agcagccact ggatatgctg ggtccgccag 120
gctccaggga aggggctgga gtggatcgca tgcatttata ctggtagtat tgatgtcttt 180
tactacgcga gctgggcgaa aggccggttc accatctcca gagacaattc caagaacacg 240
ctgtatctgc aaatgaacag cctgagagcc gaggacacgg ccgtatatta ctgtgcgaga 300
gccgctaata ctgatactac ctactttaac ttgtggggcc agggaaccct ggtcaccgtc 360
tcgagcgcta gcaccaaggg cccatcggtc ttccccctgg caccctcctc caagagcacc 420
tctgggggca cagcggccct gggctgcctg gtcaaggact acttccccga accggtgacg 480
gtgtcgtgga actcaggcgc cctgaccagc ggcgtgcaca ccttcccggc tgtcctacag 540
tcctcaggac tctactccct cagcagcgtg gtgaccgtgc cctccagcag cttgggcacc 600
cagacctaca tctgcaacgt gaatcacaag cccagcaaca ccaaggtgga caagagagtt 660
gagcccaaat cttgtgacaa aactcacaca tgcccaccgt gcccagcacc tgaagccgcg 720
ggggcaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg 780
acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc 840
aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag 900
tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat 960
ggcaaggagt acaagtgcgc ggtctccaac aaagccctcc cagcccccat cgagaaaacc 1020
atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg 1080
gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc 1140
gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct 1200
cccgtgctgg actccgacgg ctccttcttc ctctatagca agctcaccgt ggacaagagc 1260
aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac 1320
tacacgcaga agagcctctc cctgtctccg ggtggcggtg gagggtccgg cggtggtgga 1380
tcccaggtgc aattggtgga gtctggggga ggcgtggtcc agcctgggag gtccctgaga 1440
ctctcctgtg cagcctctgg attcaccttc agtagctata ctatgcactg ggtccgccag 1500
gctccaggca aggggctgga gtgggtgaca tttatatcat atgatggaaa caataaatac 1560
tacgcagact ccgtgaaggg ccgattcacc atctccagag acaattccaa gaacacgctg 1620
tatctgcaaa tgaacagcct gagagctgag gacacggcta tatattactg tgcgaggacc 1680
ggctggctgg ggccctttga ctactggggc cagggaaccc tggtcaccgt ctcgagcggt 1740
ggaggcggat ctggcggagg tggttccggc ggtggcggct ccggtggagg cggttctgaa 1800
attgtgttga cgcagtctcc aggcaccctg tctttgtctc caggggaaag agccaccctc 1860
tcctgcaggg ccagtcagag tgttggcagc agctacttag cctggtacca gcagaaacct 1920
ggccaggctc ccaggctcct catctatggt gcattcagca gggccactgg catcccagac 1980
aggttcagtg gcagtgggtc tgggacagac ttcactctca ccatcagcag actggagcct 2040
gaagattttg cagtgtatta ctgtcagcag tatggtagct caccgtggac gttcggccaa 2100
gggaccaagg tggaaatcaa a 2121
<210> 62
<211> 707
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 62
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Ser
20 25 30
His Trp Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp
35 40 45
Ile Ala Cys Ile Tyr Thr Gly Ser Ile Asp Val Phe Tyr Tyr Ala Ser
50 55 60
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr
65 70 75 80
Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr
85 90 95
Tyr Cys Ala Arg Ala Ala Asn Thr Asp Thr Thr Tyr Phe Asn Leu Trp
100 105 110
Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro
115 120 125
Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr
130 135 140
Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr
145 150 155 160
Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro
165 170 175
Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr
180 185 190
Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn
195 200 205
His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser
210 215 220
Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala
225 230 235 240
Gly Ala Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
245 250 255
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
260 265 270
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
275 280 285
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr
290 295 300
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
305 310 315 320
Gly Lys Glu Tyr Lys Cys Ala Val Ser Asn Lys Ala Leu Pro Ala Pro
325 330 335
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
340 345 350
Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val
355 360 365
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
370 375 380
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
385 390 395 400
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
405 410 415
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
420 425 430
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
435 440 445
Ser Pro Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln
450 455 460
Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg
465 470 475 480
Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Thr Met His
485 490 495
Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Thr Phe Ile
500 505 510
Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val Lys Gly Arg
515 520 525
Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met
530 535 540
Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Thr
545 550 555 560
Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr
565 570 575
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
580 585 590
Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly
595 600 605
Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala
610 615 620
Ser Gln Ser Val Gly Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro
625 630 635 640
Gly Gln Ala Pro Arg Leu Leu Ile Tyr Gly Ala Phe Ser Arg Ala Thr
645 650 655
Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr
660 665 670
Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys
675 680 685
Gln Gln Tyr Gly Ser Ser Pro Trp Thr Phe Gly Gln Gly Thr Lys Val
690 695 700
Glu Ile Lys
705
<210> 63
<211> 654
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 63
gcctatgata tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcaattgcc aggccagtca gagcattaac aaccaactat cctggtatca gcagaaacca 120
gggaaagttc ctaagctcct gatctatggt gcatccactc tggcatctgg ggtcccatct 180
cggttcaccg gcagtggatc tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagatgttg caacttatta ctgtcatgtt cattattgca gtggtggtag ttgtttttgg 300
gctttcggcg gagggaccaa ggtggagatc aaacgtacgg tggctgcacc atctgtcttc 360
atcttcccgc catctgatga gcagttgaaa tctggaactg cctctgttgt gtgcctgctg 420
aataacttct atcccagaga ggccaaagta cagtggaagg tggataacgc cctccaatcg 480
ggtaactccc aggagagtgt cacagagcag gacagcaagg acagcaccta cagcctcagc 540
agcaccctga cgctgagcaa agcagactac gagaaacaca aagtctacgc ctgcgaagtc 600
acccatcagg gcctgagctc gcccgtcaca aagagcttca acaggggaga gtgt 654
<210> 64
<211> 218
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 64
Ala Tyr Asp Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Ile Asn Asn Gln
20 25 30
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile
35 40 45
Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Thr Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Val Ala Thr Tyr Tyr Cys His Val His Tyr Cys Ser Gly Gly
85 90 95
Ser Cys Phe Trp Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg
100 105 110
Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln
115 120 125
Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr
130 135 140
Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser
145 150 155 160
Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr
165 170 175
Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys
180 185 190
His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro
195 200 205
Val Thr Lys Ser Phe Asn Arg Gly Glu Cys
210 215
<210> 65
<211> 57
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 65
atgaaacacc tgtggttctt cctcctcctg gtggcagctc ccagatgggt cctgtcc 57
<210> 66
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 66
Met Lys His Leu Trp Phe Phe Leu Leu Leu Val Ala Ala Pro Arg Trp
1 5 10 15
Val Leu Ser
<210> 67
<211> 57
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 67
atggctgtct tggggctgct cttctgcctg gtgacattcc caagctgtgt cctatcc 57
<210> 68
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 68
Met Ala Val Leu Gly Leu Leu Phe Cys Leu Val Thr Phe Pro Ser Cys
1 5 10 15
Val Leu Ser
<210> 69
<211> 60
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 69
atgagggccc ctgctcagtt tcttggcttc ttgcttttct ggattccagc ctccagaagt 60
<210> 70
<211> 20
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 70
Met Arg Ala Pro Ala Gln Phe Leu Gly Phe Leu Leu Phe Trp Ile Pro
1 5 10 15
Ala Ser Arg Ser
20
<210> 71
<211> 738
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 71
caggtgcaat tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc gagcggtgga 360
ggcggatctg gcggaggtgg ttccggcggt ggcggctccg gtggaggcgg ttctgaaatt 420
gtgttgacgc agtctccagg caccctgtct ttgtctccag gggaaagagc caccctctcc 480
tgcagggcca gtcagagtgt tggcagcagc tacttagcct ggtaccagca gaaacctggc 540
caggctccca ggctcctcat ctatggtgca ttcagcaggg ccactggcat cccagacagg 600
ttcagtggca gtgggtctgg gacagacttc actctcacca tcagcagact ggagcctgaa 660
gattttgcag tgtattactg tcagcagtat ggtagctcac cgtggacgtt cggccaaggg 720
accaaggtgg aaatcaaa 738
<210> 72
<211> 246
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 72
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
115 120 125
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln
130 135 140
Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser
145 150 155 160
Cys Arg Ala Ser Gln Ser Val Gly Ser Ser Tyr Leu Ala Trp Tyr Gln
165 170 175
Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Gly Ala Phe Ser
180 185 190
Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr
195 200 205
Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu Pro Glu Asp Phe Ala Val
210 215 220
Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro Trp Thr Phe Gly Gln Gly
225 230 235 240
Thr Lys Val Glu Ile Lys
245
<210> 73
<211> 732
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 73
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtacag cctctggatt ctccctcagt agctatgcaa tgagctgggt ccgccaggct 120
ccagggaagg ggctggagta catcggctac attggtgata ctactggcat agcctacgcg 180
agctgggcga atggcagatt caccatctcc aaagacaata ccaagaacac ggtggatctt 240
caaatgaaca gcctgagagc cgaggacacg gctgtgtatt actgtgcgag aggctggtcc 300
tacttagaca tctggggcca agggaccctg gtcaccgtct cgagcggtgg aggcggatct 360
ggcggaggtg gttccggcgg tggcggctcc ggtggaggcg gctctgccct tgtgatgacc 420
cagtctccat cctccctgtc tgcatctgta ggagacagag tcaccatcac ttgccaggcc 480
agtcagaaca tttacagcaa tttagcctgg tatcagcaga aaccagggaa agttcctaag 540
ctcctgatct atcaggcctc cactctggca tctggggtcc catctcggtt cagtggcagt 600
ggatatggga cagatttcac tctcaccatc agcagcctgc agcctgaaga tgttgcaact 660
tattactgtc aaggcggtta ttatagtgct gcccttaata ctttcggcgg agggaccaag 720
gtggagatca aa 732
<210> 74
<211> 244
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 74
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Ser Ser Tyr
20 25 30
Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Tyr Ile
35 40 45
Gly Tyr Ile Gly Asp Thr Thr Gly Ile Ala Tyr Ala Ser Trp Ala Asn
50 55 60
Gly Arg Phe Thr Ile Ser Lys Asp Asn Thr Lys Asn Thr Val Asp Leu
65 70 75 80
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Arg Gly Trp Ser Tyr Leu Asp Ile Trp Gly Gln Gly Thr Leu Val Thr
100 105 110
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gly Gly Gly Gly Ser Ala Leu Val Met Thr Gln Ser Pro Ser
130 135 140
Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Gln Ala
145 150 155 160
Ser Gln Asn Ile Tyr Ser Asn Leu Ala Trp Tyr Gln Gln Lys Pro Gly
165 170 175
Lys Val Pro Lys Leu Leu Ile Tyr Gln Ala Ser Thr Leu Ala Ser Gly
180 185 190
Val Pro Ser Arg Phe Ser Gly Ser Gly Tyr Gly Thr Asp Phe Thr Leu
195 200 205
Thr Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln
210 215 220
Gly Gly Tyr Tyr Ser Ala Ala Leu Asn Thr Phe Gly Gly Gly Thr Lys
225 230 235 240
Val Glu Ile Lys
<210> 75
<211> 750
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 75
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt cgacttcagt agcggctact ggatatgctg ggtccgccag 120
gctccaggga aggggctgga gttgatcgca tgcatttatg ctggtactag tggtagtact 180
tcctacgcga gctgggcgag aggcagattc accatctccg aaacctccaa gaacacggtg 240
actcttcaaa tgaacagcct gagagccgag gactcggctg tgtattactg tgcgagaaat 300
ctttacactt acaatagctt gtggggccag ggaaccctgg tcaccgtctc gagcggtgga 360
ggcggatctg gcggaggtgg ttccggcggt ggcggctccg gtggaggcgg ctctgacatc 420
cagatgaccc agtctccttc caccctgtct gcatctgtag gagacagagt caccatcact 480
tgccagtcca gtcagagtgt ttatgataac aactggttag cctggtatca gcagaaacca 540
gggaaagccc ctaagctcct gatctataca gtatccactc tggcatctgg ggtcccatca 600
aggttcagcg gcagtggatc tgggacagaa ttcactctca ccatcagcag cctgcagcct 660
gatgattttg caacttatta ctgccaaggc acttattata gtagtggttg gaactttgct 720
ttcggcggag ggaccaaggt ggagatcaaa 750
<210> 76
<211> 250
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 76
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Ser Gly
20 25 30
Tyr Trp Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Leu
35 40 45
Ile Ala Cys Ile Tyr Ala Gly Thr Ser Gly Ser Thr Ser Tyr Ala Ser
50 55 60
Trp Ala Arg Gly Arg Phe Thr Ile Ser Glu Thr Ser Lys Asn Thr Val
65 70 75 80
Thr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Ser Ala Val Tyr Tyr
85 90 95
Cys Ala Arg Asn Leu Tyr Thr Tyr Asn Ser Leu Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
115 120 125
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln
130 135 140
Ser Pro Ser Thr Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr
145 150 155 160
Cys Gln Ser Ser Gln Ser Val Tyr Asp Asn Asn Trp Leu Ala Trp Tyr
165 170 175
Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Thr Val Ser
180 185 190
Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly
195 200 205
Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe Ala
210 215 220
Thr Tyr Tyr Cys Gln Gly Thr Tyr Tyr Ser Ser Gly Trp Asn Phe Ala
225 230 235 240
Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
245 250
<210> 77
<211> 729
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 77
caggagcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtacag cctctggatt ctcctttagc agctactgga tgtgctgggt ccgccaggct 120
ccagggaagg ggctggagtg gatcggatgc attacgactg gtagtggtag cacttactac 180
gcgagctggg cgaagcgccg gttcaccatc tccaaagaca attccaagaa cacggtgact 240
ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtac gagagcattt 300
gacttgtggg gccagggaac cctggtcacc gtctcgagcg gtggaggcgg atctggcgga 360
ggtggttccg gcggtggcgg ctccggtgga ggcggctctg acatccagat gacccagtct 420
ccatcctccc tgtctgcatc tgtaggagac agagtcacca tcacttgcca ggccagtcag 480
agcatttaca gctacttaaa ctggtatcag cagaaaccag ggaaagcccc taagctcctg 540
atctatggtg catccaatct ggcatctggg gtcccatcaa ggttcagtgg cagtggatct 600
gggacagatt tcactctcac catcagcagt ctgcaacctg aagattttgc aacttactac 660
tgtcaaagca gttggttgag tggtgctgtt ggtaatgctt tcggcggagg gaccaaggtg 720
gagatcaaa 729
<210> 78
<211> 243
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 78
Gln Glu Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Phe Ser Ser Tyr
20 25 30
Trp Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
35 40 45
Gly Cys Ile Thr Thr Gly Ser Gly Ser Thr Tyr Tyr Ala Ser Trp Ala
50 55 60
Lys Arg Arg Phe Thr Ile Ser Lys Asp Asn Ser Lys Asn Thr Val Thr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Thr Arg Ala Phe Asp Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser
100 105 110
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
115 120 125
Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu
130 135 140
Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln
145 150 155 160
Ser Ile Tyr Ser Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala
165 170 175
Pro Lys Leu Leu Ile Tyr Gly Ala Ser Asn Leu Ala Ser Gly Val Pro
180 185 190
Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile
195 200 205
Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Ser Ser
210 215 220
Trp Leu Ser Gly Ala Val Gly Asn Ala Phe Gly Gly Gly Thr Lys Val
225 230 235 240
Glu Ile Lys
<210> 79
<211> 759
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 79
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt cacctttagc agcagccact ggatatgctg ggtccgccag 120
gctccaggga aggggctgga gtggatcgca tgcatttata ctggtagtat tgatgtcttt 180
tactacgcga gctgggcgaa aggccggttc accatctcca gagacaattc caagaacacg 240
ctgtatctgc aaatgaacag cctgagagcc gaggacacgg ccgtatatta ctgtgcgaga 300
gccgctaata ctgatactac ctactttaac ttgtggggcc agggaaccct ggtcaccgtc 360
tcgagcggtg gaggcggatc tggcggaggt ggttccggcg gtggcggctc cggtggaggc 420
ggctctgcct atgatatgac ccagtctcca tcctccctgt ctgcatctgt aggagacaga 480
gtcaccatca attgccaggc cagtcagagc attaacaacc aactatcctg gtatcagcag 540
aaaccaggga aagttcctaa gctcctgatc tatggtgcat ccactctggc atctggggtc 600
ccatctcggt tcaccggcag tggatctggg acagatttca ctctcaccat cagcagcctg 660
cagcctgaag atgttgcaac ttattactgt catgttcatt attgcagtgg tggtagttgt 720
ttttgggctt tcggcggagg gaccaaggtg gagatcaaa 759
<210> 80
<211> 253
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 80
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Ser
20 25 30
His Trp Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp
35 40 45
Ile Ala Cys Ile Tyr Thr Gly Ser Ile Asp Val Phe Tyr Tyr Ala Ser
50 55 60
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr
65 70 75 80
Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr
85 90 95
Tyr Cys Ala Arg Ala Ala Asn Thr Asp Thr Thr Tyr Phe Asn Leu Trp
100 105 110
Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
115 120 125
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Tyr
130 135 140
Asp Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg
145 150 155 160
Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Ile Asn Asn Gln Leu Ser
165 170 175
Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile Tyr Gly
180 185 190
Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Thr Gly Ser Gly
195 200 205
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp
210 215 220
Val Ala Thr Tyr Tyr Cys His Val His Tyr Cys Ser Gly Gly Ser Cys
225 230 235 240
Phe Trp Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
245 250
<210> 81
<211> 735
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 81
cagtcggtgg aggagtctgg gggaggcttg gtccagcctg gggggtccct gagactctcc 60
tgtaccgcct ctggaatcga ccttaatacc tacgacatga tctgggtccg ccaggctcca 120
ggcaaggggc tagagtgggt tggaatcatt acttatagtg gtagtagata ctacgcgaac 180
tgggcgaaag gccgattcac catctccaaa gacaatacca agaacacggt gtatctgcaa 240
atgaacagcc tgagagctga ggacacggct gtgtattact gtgcgagaga ttatatgagt 300
ggttcccact tgtggggcca gggaaccctg gtcaccgtct cttccggtgg aggcggttca 360
ggcggaggtg gaagtggtgg tggcggctct ggaggcggcg gatctgccta tgatatgacc 420
cagtctccat cttccgtgtc tgcatctgta ggagacagag tcaccatcaa gtgtcaggcc 480
agtgaggaca tttatagctt cttggcctgg tatcagcaga aaccagggaa agcccctaag 540
ctcctgatcc attctgcatc ctctctggca tctggggtcc catcaaggtt cagcggcagt 600
ggatctggga cagatttcac tctcaccatc agcagcctgc agcctgaaga ttttgcaact 660
tactattgtc aacagggtta tggtaaaaat aatgttgata atgctttcgg cggagggacc 720
aaggtggaga tcaaa 735
<210> 82
<211> 245
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 82
Gln Ser Val Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser
1 5 10 15
Leu Arg Leu Ser Cys Thr Ala Ser Gly Ile Asp Leu Asn Thr Tyr Asp
20 25 30
Met Ile Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Gly
35 40 45
Ile Ile Thr Tyr Ser Gly Ser Arg Tyr Tyr Ala Asn Trp Ala Lys Gly
50 55 60
Arg Phe Thr Ile Ser Lys Asp Asn Thr Lys Asn Thr Val Tyr Leu Gln
65 70 75 80
Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg
85 90 95
Asp Tyr Met Ser Gly Ser His Leu Trp Gly Gln Gly Thr Leu Val Thr
100 105 110
Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gly Gly Gly Gly Ser Ala Tyr Asp Met Thr Gln Ser Pro Ser
130 135 140
Ser Val Ser Ala Ser Val Gly Asp Arg Val Thr Ile Lys Cys Gln Ala
145 150 155 160
Ser Glu Asp Ile Tyr Ser Phe Leu Ala Trp Tyr Gln Gln Lys Pro Gly
165 170 175
Lys Ala Pro Lys Leu Leu Ile His Ser Ala Ser Ser Leu Ala Ser Gly
180 185 190
Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu
195 200 205
Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln
210 215 220
Gln Gly Tyr Gly Lys Asn Asn Val Asp Asn Ala Phe Gly Gly Gly Thr
225 230 235 240
Lys Val Glu Ile Lys
245
<210> 83
<211> 528
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 83
aaagacaata cctcgaccac ggtggatctg caaatgaaca gcctgagagc tgaggacacg 60
gctgtgtatt actgtgcgag agattttatt agtggttccc acttgtgggg ccagggaacc 120
ctggtcaccg tctcgagcgg tggaggcgga tctggcggag gtggttccgg cggtggcggc 180
tccggtggag gcggttctgc ctatgatatg acccagtctc catcttccgt gtctgcatct 240
gtaggagaca gagtcaccat caattgtcag gccagtgaga gcattagtag cttcttatcc 300
tggtatcagc agaaaccagg gaaagcccct aagctcctga tcttttctgc atccactctg 360
gcatctgggg tcccatcaag gttcagcggc agtggatctg ggacagattt cactctcacc 420
atcagcagcc tgcagcctga agattttgca acttactatt gtcaacaggg ttatagtaaa 480
agtaatgtgg ataatgcttt cggcggaggg accaaggtgg agatcaaa 528
<210> 84
<211> 246
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 84
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Thr Ser Gly Ile Asp Leu Ser Thr Tyr
20 25 30
Asp Met Ile Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Gly Ile Ile Ser Tyr Val Gly Asn Thr Tyr Tyr Ala Ser Trp Ala Lys
50 55 60
Gly Arg Phe Thr Leu Ser Lys Asp Asn Thr Ser Thr Thr Val Asp Leu
65 70 75 80
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Arg Asp Phe Ile Ser Gly Ser His Leu Trp Gly Gln Gly Thr Leu Val
100 105 110
Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
115 120 125
Gly Gly Ser Gly Gly Gly Gly Ser Ala Tyr Asp Met Thr Gln Ser Pro
130 135 140
Ser Ser Val Ser Ala Ser Val Gly Asp Arg Val Thr Ile Asn Cys Gln
145 150 155 160
Ala Ser Glu Ser Ile Ser Ser Phe Leu Ser Trp Tyr Gln Gln Lys Pro
165 170 175
Gly Lys Ala Pro Lys Leu Leu Ile Phe Ser Ala Ser Thr Leu Ala Ser
180 185 190
Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr
195 200 205
Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys
210 215 220
Gln Gln Gly Tyr Ser Lys Ser Asn Val Asp Asn Ala Phe Gly Gly Gly
225 230 235 240
Thr Lys Val Glu Ile Lys
245
<210> 85
<211> 756
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 85
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt ctccttcagt agcgggtacg acatgtgctg ggtccgccag 120
gctccaggga aggggctgga gtggatcgca tgcattgctg ctggtagtgc tggtatcact 180
tacgacgcga actgggcgaa aggccggttc accatctcca gagacaattc caagaacacg 240
ctgtatctgc aaatgaacag cctgagagcc gaggacacgg ccgtatatta ctgtgcgaga 300
tcggcgtttt cgttcgacta cgccatggac ctctggggcc agggaaccct ggtcaccgtc 360
tcgagcggtg gaggcggatc tggcggaggt ggttccggcg gtggcggctc cggtggaggc 420
ggctctgaca tccagatgac ccagtctcct tccaccctgt ctgcatctgt aggagacaga 480
gtcaccatca cttgccaggc cagtcagagc attagttccc acttaaactg gtatcagcag 540
aaaccaggga aagcccctaa gctcctgatc tataaggcat ccactctggc atctggggtc 600
ccatcaaggt tcagcggcag tggatctggg acagaattta ctctcaccat cagcagcctg 660
cagcctgatg attttgcaac ttattactgc caacagggtt atagttgggg taatgttgat 720
aatgttttcg gcggagggac caaggtggag atcaaa 756
<210> 86
<211> 252
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 86
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Phe Ser Ser Gly
20 25 30
Tyr Asp Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp
35 40 45
Ile Ala Cys Ile Ala Ala Gly Ser Ala Gly Ile Thr Tyr Asp Ala Asn
50 55 60
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr
65 70 75 80
Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr
85 90 95
Tyr Cys Ala Arg Ser Ala Phe Ser Phe Asp Tyr Ala Met Asp Leu Trp
100 105 110
Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
115 120 125
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile
130 135 140
Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly Asp Arg
145 150 155 160
Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Ser Ser His Leu Asn
165 170 175
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Lys
180 185 190
Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly
195 200 205
Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp
210 215 220
Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Tyr Ser Trp Gly Asn Val Asp
225 230 235 240
Asn Val Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
245 250
<210> 87
<211> 750
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 87
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt ctccctcagt agctactgga tgagctgggt ccgccaggct 120
ccagggaagg ggctggagtg gatcggagtc attgatacta atgtttatat atactacgcg 180
aactgggcaa aaggcagatt caccatctcc agagacaatt ccaagaacac gctgtatctt 240
caaatgaaca gcctgagagc cgaggacacg gctgtgtatt actgtgcgag atatgtgggt 300
aataatgatg attatattaa cttgtggggc cagggaaccc tggtcaccgt ctcgagcggt 360
ggaggcggat ctggcggagg tggttccggc ggtggcggct ccggtggagg cggctctgac 420
atccagatga cccagtctcc ttccaccctg tctgcatctg taggagacag agtcaccatc 480
acttgccagt ccagtcagag tgtttataat ggctactggt tatcctggta tcagcagaaa 540
ccagggaaag cccctaagct cctgatctat ggtgcatcca ctctggcatc tggggtccca 600
tcaaggttca gcggcagtgg atctgggaca gaattcactc tcaccatcag cagcctgcag 660
cctgatgatt ttgcaactta ttactgccta ggcagttata ctagtagtac tgagaactct 720
ttcggcggag ggaccaaggt ggagatcaaa 750
<210> 88
<211> 250
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 88
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser Tyr
20 25 30
Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
35 40 45
Gly Val Ile Asp Thr Asn Val Tyr Ile Tyr Tyr Ala Asn Trp Ala Lys
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Arg Tyr Val Gly Asn Asn Asp Asp Tyr Ile Asn Leu Trp Gly Gln Gly
100 105 110
Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
115 120 125
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr
130 135 140
Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile
145 150 155 160
Thr Cys Gln Ser Ser Gln Ser Val Tyr Asn Gly Tyr Trp Leu Ser Trp
165 170 175
Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Gly Ala
180 185 190
Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser
195 200 205
Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp Phe
210 215 220
Ala Thr Tyr Tyr Cys Leu Gly Ser Tyr Thr Ser Ser Thr Glu Asn Ser
225 230 235 240
Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
245 250
<210> 89
<211> 741
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 89
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtacag tgtctggaat cgacctcagt gtcatcaata tgggctgggt ccgccaggct 120
ccagggaagg ggctggagtg gatcggaacc attacttatg ttggtaacac atattacgcg 180
agctgggcga aaggcagatt caccatctcc aaaacctcga ccacggtgga tcttaaaatc 240
accagtccga caaccgagga cacggctgtg tattactgtg cgagagaatc tggtactatt 300
tattacagtt actttaactt gtggggccaa gggaccctgg tcaccgtctc gagcggtgga 360
ggcggatctg gcggaggtgg ttccggcggt ggcggctccg gtggaggcgg ctctgcattc 420
gaattgaccc agtctccatc ctccctgtct gcatctgtag gagacagagt caccatcaag 480
tgccaggcca gtgaaagcat tagcaactac ttatcctggt atcagcagat tccagggaaa 540
gttcctaagc tcctgatcta ttatgcatcc aatctggcat ctggggtccc atctcggttc 600
agtggcagtg gatctgggac agatttcact ctcaccatca gcagcctgca gcctgaagat 660
gttgcaactt attactgtca aagctattat ggtggtggta gtgcctatac tttcggcgga 720
gggaccaagg tggagatcaa a 741
<210> 90
<211> 247
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 90
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Val Ser Gly Ile Asp Leu Ser Val Ile
20 25 30
Asn Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
35 40 45
Gly Thr Ile Thr Tyr Val Gly Asn Thr Tyr Tyr Ala Ser Trp Ala Lys
50 55 60
Gly Arg Phe Thr Ile Ser Lys Thr Ser Thr Thr Val Asp Leu Lys Ile
65 70 75 80
Thr Ser Pro Thr Thr Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu
85 90 95
Ser Gly Thr Ile Tyr Tyr Ser Tyr Phe Asn Leu Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
115 120 125
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Phe Glu Leu Thr Gln
130 135 140
Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Lys
145 150 155 160
Cys Gln Ala Ser Glu Ser Ile Ser Asn Tyr Leu Ser Trp Tyr Gln Gln
165 170 175
Ile Pro Gly Lys Val Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Asn Leu
180 185 190
Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp
195 200 205
Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr
210 215 220
Tyr Cys Gln Ser Tyr Tyr Gly Gly Gly Ser Ala Tyr Thr Phe Gly Gly
225 230 235 240
Gly Thr Lys Val Glu Ile Lys
245
<210> 91
<211> 354
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 91
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc 60
tcctgtgcag cctctggatt caccttcagt agctatacta tgcactgggt ccgccaggct 120
ccaggcaagg ggctggagtg ggtgacattt atatcatatg atggaaacaa taaatactac 180
gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat 240
ctgcaaatga acagcctgag agctgaggac acggctatat attactgtgc gaggaccggc 300
tggctggggc cctttgacta ctggggccag ggaaccctgg tcaccgtctc ctca 354
<210> 92
<211> 118
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 92
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
20 25 30
Thr Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Thr Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val
50 55 60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Arg Thr Gly Trp Leu Gly Pro Phe Asp Tyr Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser
115
<210> 93
<211> 324
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 93
gaaattgtgt tgacgcagtc tccaggcacc ctgtctttgt ctccagggga aagagccacc 60
ctctcctgca gggccagtca gagtgttggc agcagctact tagcctggta ccagcagaaa 120
cctggccagg ctcccaggct cctcatctat ggtgcattca gcagggccac tggcatccca 180
gacaggttca gtggcagtgg gtctgggaca gacttcactc tcaccatcag cagactggag 240
cctgaagatt ttgcagtgta ttactgtcag cagtatggta gctcaccgtg gacgttcggc 300
caagggacca aggtggaaat caaa 324
<210> 94
<211> 108
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 94
Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly
1 5 10 15
Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser Ser
20 25 30
Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu
35 40 45
Ile Tyr Gly Ala Phe Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser
50 55 60
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Arg Leu Glu
65 70 75 80
Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Gly Ser Ser Pro
85 90 95
Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
100 105
<210> 95
<211> 345
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 95
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtacag cctctggatt ctccctcagt agctatgcaa tgagctgggt ccgccaggct 120
ccagggaagg ggctggagta catcggctac attggtgata ctactggcat agcctacgcg 180
agctgggcga atggcagatt caccatctcc aaagacaata ccaagaacac ggtggatctt 240
caaatgaaca gcctgagagc cgaggacacg gctgtgtatt actgtgcgag aggctggtcc 300
tacttagaca tctggggcca agggaccctg gtcaccgtct cgagc 345
<210> 96
<211> 115
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 96
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Ser Ser Tyr
20 25 30
Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Tyr Ile
35 40 45
Gly Tyr Ile Gly Asp Thr Thr Gly Ile Ala Tyr Ala Ser Trp Ala Asn
50 55 60
Gly Arg Phe Thr Ile Ser Lys Asp Asn Thr Lys Asn Thr Val Asp Leu
65 70 75 80
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Arg Gly Trp Ser Tyr Leu Asp Ile Trp Gly Gln Gly Thr Leu Val Thr
100 105 110
Val Ser Ser
115
<210> 97
<211> 327
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 97
gcccttgtga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc aggccagtca gaacatttac agcaatttag cctggtatca gcagaaacca 120
gggaaagttc ctaagctcct gatctatcag gcctccactc tggcatctgg ggtcccatct 180
cggttcagtg gcagtggata tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagatgttg caacttatta ctgtcaaggc ggttattata gtgctgccct taatactttc 300
ggcggaggga ccaaggtgga gatcaaa 327
<210> 98
<211> 109
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 98
Ala Leu Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Asn Ile Tyr Ser Asn
20 25 30
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile
35 40 45
Tyr Gln Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Tyr Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gly Gly Tyr Tyr Ser Ala Ala
85 90 95
Leu Asn Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105
<210> 99
<211> 354
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 99
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt cgacttcagt agcggctact ggatatgctg ggtccgccag 120
gctccaggga aggggctgga gttgatcgca tgcatttatg ctggtactag tggtagtact 180
tcctacgcga gctgggcgag aggcagattc accatctccg aaacctccaa gaacacggtg 240
actcttcaaa tgaacagcct gagagccgag gactcggctg tgtattactg tgcgagaaat 300
ctttacactt acaatagctt gtggggccag ggaaccctgg tcaccgtctc gagc 354
<210> 100
<211> 118
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 100
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Ser Gly
20 25 30
Tyr Trp Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Leu
35 40 45
Ile Ala Cys Ile Tyr Ala Gly Thr Ser Gly Ser Thr Ser Tyr Ala Ser
50 55 60
Trp Ala Arg Gly Arg Phe Thr Ile Ser Glu Thr Ser Lys Asn Thr Val
65 70 75 80
Thr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Ser Ala Val Tyr Tyr
85 90 95
Cys Ala Arg Asn Leu Tyr Thr Tyr Asn Ser Leu Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser
115
<210> 101
<211> 336
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 101
gacatccaga tgacccagtc tccttccacc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc agtccagtca gagtgtttat gataacaact ggttagcctg gtatcagcag 120
aaaccaggga aagcccctaa gctcctgatc tatacagtat ccactctggc atctggggtc 180
ccatcaaggt tcagcggcag tggatctggg acagaattca ctctcaccat cagcagcctg 240
cagcctgatg attttgcaac ttattactgc caaggcactt attatagtag tggttggaac 300
tttgctttcg gcggagggac caaggtggag atcaaa 336
<210> 102
<211> 112
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 102
Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Gln Ser Val Tyr Asp Asn
20 25 30
Asn Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu
35 40 45
Leu Ile Tyr Thr Val Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe
50 55 60
Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu
65 70 75 80
Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gly Thr Tyr Tyr Ser
85 90 95
Ser Gly Trp Asn Phe Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105 110
<210> 103
<211> 339
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 103
caggagcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtacag cctctggatt ctcctttagc agctactgga tgtgctgggt ccgccaggct 120
ccagggaagg ggctggagtg gatcggatgc attacgactg gtagtggtag cacttactac 180
gcgagctggg cgaagcgccg gttcaccatc tccaaagaca attccaagaa cacggtgact 240
ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtac gagagcattt 300
gacttgtggg gccagggaac cctggtcacc gtctcgagc 339
<210> 104
<211> 113
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 104
Gln Glu Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Phe Ser Ser Tyr
20 25 30
Trp Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
35 40 45
Gly Cys Ile Thr Thr Gly Ser Gly Ser Thr Tyr Tyr Ala Ser Trp Ala
50 55 60
Lys Arg Arg Phe Thr Ile Ser Lys Asp Asn Ser Lys Asn Thr Val Thr
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Thr Arg Ala Phe Asp Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser
100 105 110
Ser
<210> 105
<211> 330
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 105
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc aggccagtca gagcatttac agctacttaa actggtatca gcagaaacca 120
gggaaagccc ctaagctcct gatctatggt gcatccaatc tggcatctgg ggtcccatca 180
aggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct 240
gaagattttg caacttacta ctgtcaaagc agttggttga gtggtgctgt tggtaatgct 300
ttcggcggag ggaccaaggt ggagatcaaa 330
<210> 106
<211> 110
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 106
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Tyr Ser Tyr
20 25 30
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
35 40 45
Tyr Gly Ala Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Ser Ser Trp Leu Ser Gly Ala
85 90 95
Val Gly Asn Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105 110
<210> 107
<211> 366
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 107
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt cacctttagc agcagccact ggatatgctg ggtccgccag 120
gctccaggga aggggctgga gtggatcgca tgcatttata ctggtagtat tgatgtcttt 180
tactacgcga gctgggcgaa aggccggttc accatctcca gagacaattc caagaacacg 240
ctgtatctgc aaatgaacag cctgagagcc gaggacacgg ccgtatatta ctgtgcgaga 300
gccgctaata ctgatactac ctactttaac ttgtggggcc agggaaccct ggtcaccgtc 360
tcgagc 366
<210> 108
<211> 122
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 108
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Ser
20 25 30
His Trp Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp
35 40 45
Ile Ala Cys Ile Tyr Thr Gly Ser Ile Asp Val Phe Tyr Tyr Ala Ser
50 55 60
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr
65 70 75 80
Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr
85 90 95
Tyr Cys Ala Arg Ala Ala Asn Thr Asp Thr Thr Tyr Phe Asn Leu Trp
100 105 110
Gly Gln Gly Thr Leu Val Thr Val Ser Ser
115 120
<210> 109
<211> 333
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 109
gcctatgata tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcaattgcc aggccagtca gagcattaac aaccaactat cctggtatca gcagaaacca 120
gggaaagttc ctaagctcct gatctatggt gcatccactc tggcatctgg ggtcccatct 180
cggttcaccg gcagtggatc tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagatgttg caacttatta ctgtcatgtt cattattgca gtggtggtag ttgtttttgg 300
gctttcggcg gagggaccaa ggtggagatc aaa 333
<210> 110
<211> 111
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 110
Ala Tyr Asp Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Ile Asn Asn Gln
20 25 30
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile
35 40 45
Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Thr Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Val Ala Thr Tyr Tyr Cys His Val His Tyr Cys Ser Gly Gly
85 90 95
Ser Cys Phe Trp Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105 110
<210> 111
<211> 345
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 111
cagtcggtgg aggagtctgg gggaggcttg gtccagcctg gggggtccct gagactctcc 60
tgtaccgcct ctggaatcga ccttaatacc tacgacatga tctgggtccg ccaggctcca 120
ggcaaggggc tagagtgggt tggaatcatt acttatagtg gtagtagata ctacgcgaac 180
tgggcgaaag gccgattcac catctccaaa gacaatacca agaacacggt gtatctgcaa 240
atgaacagcc tgagagctga ggacacggct gtgtattact gtgcgagaga ttatatgagt 300
ggttcccact tgtggggcca gggaaccctg gtcaccgtct cttcc 345
<210> 112
<211> 115
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 112
Gln Ser Val Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser
1 5 10 15
Leu Arg Leu Ser Cys Thr Ala Ser Gly Ile Asp Leu Asn Thr Tyr Asp
20 25 30
Met Ile Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Gly
35 40 45
Ile Ile Thr Tyr Ser Gly Ser Arg Tyr Tyr Ala Asn Trp Ala Lys Gly
50 55 60
Arg Phe Thr Ile Ser Lys Asp Asn Thr Lys Asn Thr Val Tyr Leu Gln
65 70 75 80
Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg
85 90 95
Asp Tyr Met Ser Gly Ser His Leu Trp Gly Gln Gly Thr Leu Val Thr
100 105 110
Val Ser Ser
115
<210> 113
<211> 330
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 113
gcctatgata tgacccagtc tccatcttcc gtgtctgcat ctgtaggaga cagagtcacc 60
atcaagtgtc aggccagtga ggacatttat agcttcttgg cctggtatca gcagaaacca 120
gggaaagccc ctaagctcct gatccattct gcatcctctc tggcatctgg ggtcccatca 180
aggttcagcg gcagtggatc tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagattttg caacttacta ttgtcaacag ggttatggta aaaataatgt tgataatgct 300
ttcggcggag ggaccaaggt ggagatcaaa 330
<210> 114
<211> 110
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 114
Ala Tyr Asp Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Lys Cys Gln Ala Ser Glu Asp Ile Tyr Ser Phe
20 25 30
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
35 40 45
His Ser Ala Ser Ser Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Tyr Gly Lys Asn Asn
85 90 95
Val Asp Asn Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105 110
<210> 115
<211> 348
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 115
caggtgcagc tggtggagtc tgggggaggc ctggtccagc ctgggggctc cctgagactc 60
tcctgtacaa cttctggaat cgaccttagt acctacgaca tgatctgggt ccgccaggct 120
ccaggcaagg ggctagagtg ggtgggaatc attagttatg ttggtaacac atactacgcg 180
agctgggcga aaggccgatt caccctctcc aaagacaata cctcgaccac ggtggatctg 240
caaatgaaca gcctgagagc tgaggacacg gctgtgtatt actgtgcgag agattttatt 300
agtggttccc acttgtgggg ccagggaacc ctggtcaccg tctcgagc 348
<210> 116
<211> 116
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 116
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Thr Ser Gly Ile Asp Leu Ser Thr Tyr
20 25 30
Asp Met Ile Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Gly Ile Ile Ser Tyr Val Gly Asn Thr Tyr Tyr Ala Ser Trp Ala Lys
50 55 60
Gly Arg Phe Thr Leu Ser Lys Asp Asn Thr Ser Thr Thr Val Asp Leu
65 70 75 80
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Arg Asp Phe Ile Ser Gly Ser His Leu Trp Gly Gln Gly Thr Leu Val
100 105 110
Thr Val Ser Ser
115
<210> 117
<211> 330
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 117
gcctatgata tgacccagtc tccatcttcc gtgtctgcat ctgtaggaga cagagtcacc 60
atcaattgtc aggccagtga gagcattagt agcttcttat cctggtatca gcagaaacca 120
gggaaagccc ctaagctcct gatcttttct gcatccactc tggcatctgg ggtcccatca 180
aggttcagcg gcagtggatc tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagattttg caacttacta ttgtcaacag ggttatagta aaagtaatgt ggataatgct 300
ttcggcggag ggaccaaggt ggagatcaaa 330
<210> 118
<211> 110
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 118
Ala Tyr Asp Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Glu Ser Ile Ser Ser Phe
20 25 30
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
35 40 45
Phe Ser Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Tyr Ser Lys Ser Asn
85 90 95
Val Asp Asn Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105 110
<210> 119
<211> 366
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 119
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt ctccttcagt agcgggtacg acatgtgctg ggtccgccag 120
gctccaggga aggggctgga gtggatcgca tgcattgctg ctggtagtgc tggtatcact 180
tacgacgcga actgggcgaa aggccggttc accatctcca gagacaattc caagaacacg 240
ctgtatctgc aaatgaacag cctgagagcc gaggacacgg ccgtatatta ctgtgcgaga 300
tcggcgtttt cgttcgacta cgccatggac ctctggggcc agggaaccct ggtcaccgtc 360
tcgagc 366
<210> 120
<211> 122
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 120
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Phe Ser Ser Gly
20 25 30
Tyr Asp Met Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp
35 40 45
Ile Ala Cys Ile Ala Ala Gly Ser Ala Gly Ile Thr Tyr Asp Ala Asn
50 55 60
Trp Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr
65 70 75 80
Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr
85 90 95
Tyr Cys Ala Arg Ser Ala Phe Ser Phe Asp Tyr Ala Met Asp Leu Trp
100 105 110
Gly Gln Gly Thr Leu Val Thr Val Ser Ser
115 120
<210> 121
<211> 330
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 121
gacatccaga tgacccagtc tccttccacc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc aggccagtca gagcattagt tcccacttaa actggtatca gcagaaacca 120
gggaaagccc ctaagctcct gatctataag gcatccactc tggcatctgg ggtcccatca 180
aggttcagcg gcagtggatc tgggacagaa tttactctca ccatcagcag cctgcagcct 240
gatgattttg caacttatta ctgccaacag ggttatagtt ggggtaatgt tgataatgtt 300
ttcggcggag ggaccaaggt ggagatcaaa 330
<210> 122
<211> 110
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 122
Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Ser Ser His
20 25 30
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
35 40 45
Tyr Lys Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Tyr Ser Trp Gly Asn
85 90 95
Val Asp Asn Val Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105 110
<210> 123
<211> 357
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 123
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt ctccctcagt agctactgga tgagctgggt ccgccaggct 120
ccagggaagg ggctggagtg gatcggagtc attgatacta atgtttatat atactacgcg 180
aactgggcaa aaggcagatt caccatctcc agagacaatt ccaagaacac gctgtatctt 240
caaatgaaca gcctgagagc cgaggacacg gctgtgtatt actgtgcgag atatgtgggt 300
aataatgatg attatattaa cttgtggggc cagggaaccc tggtcaccgt ctcgagc 357
<210> 124
<211> 119
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 124
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser Tyr
20 25 30
Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
35 40 45
Gly Val Ile Asp Thr Asn Val Tyr Ile Tyr Tyr Ala Asn Trp Ala Lys
50 55 60
Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu
65 70 75 80
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala
85 90 95
Arg Tyr Val Gly Asn Asn Asp Asp Tyr Ile Asn Leu Trp Gly Gln Gly
100 105 110
Thr Leu Val Thr Val Ser Ser
115
<210> 125
<211> 333
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 125
gacatccaga tgacccagtc tccttccacc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc agtccagtca gagtgtttat aatggctact ggttatcctg gtatcagcag 120
aaaccaggga aagcccctaa gctcctgatc tatggtgcat ccactctggc atctggggtc 180
ccatcaaggt tcagcggcag tggatctggg acagaattca ctctcaccat cagcagcctg 240
cagcctgatg attttgcaac ttattactgc ctaggcagtt atactagtag tactgagaac 300
tctttcggcg gagggaccaa ggtggagatc aaa 333
<210> 126
<211> 111
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 126
Asp Ile Gln Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Gln Ser Val Tyr Asn Gly
20 25 30
Tyr Trp Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu
35 40 45
Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe
50 55 60
Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu
65 70 75 80
Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Leu Gly Ser Tyr Thr Ser
85 90 95
Ser Thr Glu Asn Ser Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105 110
<210> 127
<211> 354
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 127
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60
tcctgtacag tgtctggaat cgacctcagt gtcatcaata tgggctgggt ccgccaggct 120
ccagggaagg ggctggagtg gatcggaacc attacttatg ttggtaacac atattacgcg 180
agctgggcga aaggcagatt caccatctcc aaaacctcga ccacggtgga tcttaaaatc 240
accagtccga caaccgagga cacggctgtg tattactgtg cgagagaatc tggtactatt 300
tattacagtt actttaactt gtggggccaa gggaccctgg tcaccgtctc gagc 354
<210> 128
<211> 118
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 128
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Thr Val Ser Gly Ile Asp Leu Ser Val Ile
20 25 30
Asn Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile
35 40 45
Gly Thr Ile Thr Tyr Val Gly Asn Thr Tyr Tyr Ala Ser Trp Ala Lys
50 55 60
Gly Arg Phe Thr Ile Ser Lys Thr Ser Thr Thr Val Asp Leu Lys Ile
65 70 75 80
Thr Ser Pro Thr Thr Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Glu
85 90 95
Ser Gly Thr Ile Tyr Tyr Ser Tyr Phe Asn Leu Trp Gly Gln Gly Thr
100 105 110
Leu Val Thr Val Ser Ser
115
<210> 129
<211> 327
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 129
gcattcgaat tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcaagtgcc aggccagtga aagcattagc aactacttat cctggtatca gcagattcca 120
gggaaagttc ctaagctcct gatctattat gcatccaatc tggcatctgg ggtcccatct 180
cggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag cctgcagcct 240
gaagatgttg caacttatta ctgtcaaagc tattatggtg gtggtagtgc ctatactttc 300
ggcggaggga ccaaggtgga gatcaaa 327
<210> 130
<211> 109
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 130
Ala Phe Glu Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Lys Cys Gln Ala Ser Glu Ser Ile Ser Asn Tyr
20 25 30
Leu Ser Trp Tyr Gln Gln Ile Pro Gly Lys Val Pro Lys Leu Leu Ile
35 40 45
Tyr Tyr Ala Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65 70 75 80
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Ser Tyr Tyr Gly Gly Gly Ser
85 90 95
Ala Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105
<210> 131
<211> 339
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 131
caggagcagg tgaaggagac cgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt caccatcagc agctatggag tgagctgggt ccgccaggct 120
ccagggaagg ggctggagtg ggtcgcattg atttttcccg ggattggttt caaagactac 180
gcgagctggg tgaatggccg gttcaccctc tccagcgaca acgcccagaa cactgtggaa 240
ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gagagatttg 300
gacttgtggg gccagggaac cctggtcacc gtctcgagc 339
<210> 132
<211> 113
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 132
Gln Glu Gln Val Lys Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Ile Ser Ser Tyr
20 25 30
Gly Val Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Ala Leu Ile Phe Pro Gly Ile Gly Phe Lys Asp Tyr Ala Ser Trp Val
50 55 60
Asn Gly Arg Phe Thr Leu Ser Ser Asp Asn Ala Gln Asn Thr Val Glu
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Arg Asp Leu Asp Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser
100 105 110
Ser
<210> 133
<211> 327
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 133
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc 60
atcacttgcc agtccagtcc gagtgtttat agtaactact tatcctggta tcagcagaaa 120
ccagggaaag ttcctaagct cctgatctat tatgcatcca ctctggcatc tggggtccca 180
tctcggttca gtggcagtgg atctgggaca gatttcactc tcaccatcag cagcctgcag 240
cctgaagatg ttgcaactta ttactgtgca ggcggttata gtagtagtac tcgtgctttc 300
ggcggaggga ccaaggtgga gatcaaa 327
<210> 134
<211> 109
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 134
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
1 5 10 15
Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Pro Ser Val Tyr Ser Asn
20 25 30
Tyr Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu
35 40 45
Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser
50 55 60
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
65 70 75 80
Pro Glu Asp Val Ala Thr Tyr Tyr Cys Ala Gly Gly Tyr Ser Ser Ser
85 90 95
Thr Arg Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys
100 105
<210> 135
<211> 987
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 135
gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg 60
ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg 120
tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca 180
ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc 240
tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagag agttgagccc 300
aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga 360
ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct 420
gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg 480
tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac 540
agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag 600
gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc 660
aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag 720
ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc 780
gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg 840
ctggactccg acggctcctt cttcctctat agcaagctca ccgtggacaa gagcaggtgg 900
cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg 960
cagaagagcc tctccctgtc tccgggt 987
<210> 136
<211> 329
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 136
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
1 5 10 15
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
20 25 30
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
35 40 45
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
50 55 60
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
65 70 75 80
Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
85 90 95
Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
100 105 110
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
115 120 125
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
130 135 140
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145 150 155 160
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
165 170 175
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
180 185 190
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
195 200 205
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
210 215 220
Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
225 230 235 240
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
245 250 255
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
260 265 270
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
275 280 285
Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
290 295 300
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305 310 315 320
Gln Lys Ser Leu Ser Leu Ser Pro Gly
325
<210> 137
<211> 987
<212> DNA
<213> Artificial Sequence
<220>
<223> synthesized
<400> 137
gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg 60
ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg 120
tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca 180
ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc 240
tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagag agttgagccc 300
aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaagc cgcgggggca 360
ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct 420
gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg 480
tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac 540
agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag 600
gagtacaagt gcgcggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc 660
aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag 720
ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc 780
gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg 840
ctggactccg acggctcctt cttcctctat agcaagctca ccgtggacaa gagcaggtgg 900
cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg 960
cagaagagcc tctccctgtc tccgggt 987
<210> 138
<211> 329
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 138
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
1 5 10 15
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
20 25 30
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
35 40 45
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
50 55 60
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
65 70 75 80
Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
85 90 95
Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
100 105 110
Pro Ala Pro Glu Ala Ala Gly Ala Pro Ser Val Phe Leu Phe Pro Pro
115 120 125
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
130 135 140
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145 150 155 160
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
165 170 175
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
180 185 190
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Ala Val Ser Asn
195 200 205
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
210 215 220
Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
225 230 235 240
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
245 250 255
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
260 265 270
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
275 280 285
Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
290 295 300
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305 310 315 320
Gln Lys Ser Leu Ser Leu Ser Pro Gly
325
<210> 139
<211> 726
<212> DNA
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 139
caggagcagg tgaaggagac cgggggaggc ttggtacagc ctggggggtc cctgagactc 60
tcctgtgcag cctctggatt caccatcagc agctatggag tgagctgggt ccgccaggct 120
ccagggaagg ggctggagtg ggtcgcattg atttttcccg ggattggttt caaagactac 180
gcgagctggg tgaatggccg gttcaccctc tccagcgaca acgcccagaa cactgtggaa 240
ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gagagatttg 300
gacttgtggg gccagggaac cctggtcacc gtctcgagcg gtggaggcgg atctggcgga 360
ggtggttccg gcggtggcgg ctccggtgga ggcggctctg acatccagat gacccagtct 420
ccatcctccc tgtctgcatc tgtaggagac agagtcacca tcacttgcca gtccagtccg 480
agtgtttata gtaactactt atcctggtat cagcagaaac cagggaaagt tcctaagctc 540
ctgatctatt atgcatccac tctggcatct ggggtcccat ctcggttcag tggcagtgga 600
tctgggacag atttcactct caccatcagc agcctgcagc ctgaagatgt tgcaacttat 660
tactgtgcag gcggttatag tagtagtact cgtgctttcg gcggagggac caaggtggag 720
atcaaa 726
<210> 140
<211> 242
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 140
Gln Glu Gln Val Lys Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Ile Ser Ser Tyr
20 25 30
Gly Val Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
35 40 45
Ala Leu Ile Phe Pro Gly Ile Gly Phe Lys Asp Tyr Ala Ser Trp Val
50 55 60
Asn Gly Arg Phe Thr Leu Ser Ser Asp Asn Ala Gln Asn Thr Val Glu
65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
85 90 95
Ala Arg Asp Leu Asp Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser
100 105 110
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
115 120 125
Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu
130 135 140
Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Pro
145 150 155 160
Ser Val Tyr Ser Asn Tyr Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys
165 170 175
Val Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Thr Leu Ala Ser Gly Val
180 185 190
Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
195 200 205
Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Ala Gly
210 215 220
Gly Tyr Ser Ser Ser Thr Arg Ala Phe Gly Gly Gly Thr Lys Val Glu
225 230 235 240
Ile Lys
<210> 141
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 141
Ser Ser Tyr Thr Met His
1 5
<210> 142
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 142
Phe Ile Ser Tyr Asp Gly Asn Asn Lys Tyr Tyr Ala Asp Ser Val Lys
1 5 10 15
Gly
<210> 143
<211> 9
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 143
Thr Gly Trp Leu Gly Pro Phe Asp Tyr
1 5
<210> 144
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 144
Arg Ala Ser Gln Ser Val Gly Ser Ser Tyr Leu Ala
1 5 10
<210> 145
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 145
Gly Ala Phe Ser Arg Ala Thr
1 5
<210> 146
<211> 9
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 146
Gln Gln Tyr Gly Ser Ser Pro Trp Thr
1 5
<210> 147
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 147
Asn Thr Tyr Asp Met Ile
1 5
<210> 148
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 148
Ile Ile Thr Tyr Ser Gly Ser Arg Tyr Tyr Ala Asn Trp Ala Lys Gly
1 5 10 15
<210> 149
<211> 8
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 149
Asp Tyr Met Ser Gly Ser His Leu
1 5
<210> 150
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 150
Gln Ala Ser Glu Asp Ile Tyr Ser Phe Leu Ala
1 5 10
<210> 151
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 151
Ser Ala Ser Ser Leu Ala Ser
1 5
<210> 152
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 152
Gln Gln Gly Tyr Gly Lys Asn Asn Val Asp Asn Ala
1 5 10
<210> 153
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 153
Asn Thr Tyr Asp Met Ile
1 5
<210> 154
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 154
Ile Ile Thr Tyr Ser Gly Ser Arg Tyr Tyr Ala Asn Trp Ala Lys Gly
1 5 10 15
<210> 155
<211> 8
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 155
Asp Tyr Met Ser Gly Ser His Leu
1 5
<210> 156
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 156
Gln Ala Ser Glu Asp Ile Tyr Ser Phe Leu Ala
1 5 10
<210> 157
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 157
Ser Ala Ser Ser Leu Ala Ser
1 5
<210> 158
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 158
Gln Gln Gly Tyr Gly Lys Asn Asn Val Asp Asn Ala
1 5 10
<210> 159
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 159
Ser Gly Tyr Asp Met Cys
1 5
<210> 160
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 160
Cys Ile Ala Ala Gly Ser Ala Gly Ile Thr Tyr Asp Ala Asn Trp Ala
1 5 10 15
Lys Gly
<210> 161
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 161
Ser Ala Phe Ser Phe Asp Tyr Ala Met Asp Leu
1 5 10
<210> 162
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 162
Gln Ala Ser Gln Ser Ile Ser Ser His Leu Asn
1 5 10
<210> 163
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 163
Lys Ala Ser Thr Leu Ala Ser
1 5
<210> 164
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 164
Gln Gln Gly Tyr Ser Trp Gly Asn Val Asp Asn Val
1 5 10
<210> 165
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 165
Ser Thr Tyr Asp Met Ile
1 5
<210> 166
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 166
Ile Ile Ser Tyr Val Gly Asn Thr Tyr Tyr Ala Ser Trp Ala Lys Gly
1 5 10 15
<210> 167
<211> 8
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 167
Asp Phe Ile Ser Gly Ser His Leu
1 5
<210> 168
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 168
Gln Ala Ser Glu Ser Ile Ser Ser Phe Leu Ser
1 5 10
<210> 169
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 169
Ser Ala Ser Thr Leu Ala Ser
1 5
<210> 170
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 170
Gln Gln Gly Tyr Ser Lys Ser Asn Val Asp Asn Ala
1 5 10
<210> 171
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 171
Ser Ser Tyr Trp Met Ser
1 5
<210> 172
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 172
Val Ile Asp Thr Asn Val Tyr Ile Tyr Tyr Ala Asn Trp Ala Lys Gly
1 5 10 15
<210> 173
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 173
Tyr Val Gly Asn Asn Asp Asp Tyr Ile Asn Leu
1 5 10
<210> 174
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 174
Gln Ser Ser Gln Ser Val Tyr Asn Gly Trp Leu Ser
1 5 10
<210> 175
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 175
Gly Ala Ser Thr Leu Ala Ser
1 5
<210> 176
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 176
Leu Gly Ser Tyr Thr Ser Ser Thr Glu Asn Ser
1 5 10
<210> 177
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 177
Ser Val Ile Asn Met Gly
1 5
<210> 178
<211> 16
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 178
Thr Ile Thr Tyr Val Gly Asn Thr Tyr Tyr Ala Ser Trp Ala Lys Gly
1 5 10 15
<210> 179
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 179
Glu Ser Gly Thr Ile Tyr Tyr Ser Tyr Phe Asn Leu
1 5 10
<210> 180
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 180
Gln Ala Ser Glu Ser Ile Ser Asn Tyr Leu Ser
1 5 10
<210> 181
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 181
Tyr Ala Ser Asn Leu Ala Ser
1 5
<210> 182
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 182
Gln Ser Tyr Tyr Gly Gly Gly Ser Ala Tyr Thr
1 5 10
<210> 183
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 183
Ser Gly Tyr Trp Ile Cys
1 5
<210> 184
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 184
Cys Ile Tyr Ala Gly Thr Ser Gly Ser Thr Ser Tyr Ala Ser Trp Ala
1 5 10 15
Arg Gly
<210> 185
<211> 8
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 185
Asn Leu Tyr Thr Tyr Asn Ser Leu
1 5
<210> 186
<211> 13
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 186
Gln Ser Ser Gln Ser Val Tyr Asp Asn Asn Trp Leu Ala
1 5 10
<210> 187
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 187
Thr Val Ser Thr Leu Ala Ser
1 5
<210> 188
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 188
Gln Gly Thr Tyr Tyr Ser Ser Gly Trp Asn Phe Ala
1 5 10
<210> 189
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 189
Ser Ser Tyr Trp Met Cys
1 5
<210> 190
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 190
Cys Ile Thr Thr Gly Ser Gly Ser Thr Tyr Tyr Ala Ser Trp Ala Lys
1 5 10 15
Arg
<210> 191
<211> 4
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 191
Ala Phe Asp Leu
1
<210> 192
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 192
Gln Ala Ser Gln Ser Ile Tyr Ser Tyr Leu Asn
1 5 10
<210> 193
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 193
Gly Ala Ser Asn Leu Ala Ser
1 5
<210> 194
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 194
Gln Ser Ser Trp Leu Ser Gly Ala Val Gly Asn Ala
1 5 10
<210> 195
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 195
Ser Ser Tyr Gly Val Ser
1 5
<210> 196
<211> 17
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 196
Leu Ile Phe Pro Gly Ile Gly Phe Lys Asp Tyr Ala Ser Trp Val Asn
1 5 10 15
Gly
<210> 197
<211> 4
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 197
Asp Leu Asp Leu
1
<210> 198
<211> 12
<212> PRT
<213> Artificial Sequence
<220>
<223> synthesized
<400> 198
Gln Ser Ser Pro Ser Val Tyr Ser Asn Tyr Leu Ser
1 5 10
<210> 199
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 199
Tyr Ala Ser Thr Leu Ala Ser
1 5
<210> 200
<211> 10
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 200
Ala Gly Gly Tyr Ser Ser Ser Thr Arg Ala
1 5 10
<210> 201
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 201
Ser Ser His Trp Ile Cys
1 5
<210> 202
<211> 18
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 202
Cys Ile Tyr Thr Gly Ser Ile Asp Val Phe Tyr Tyr Ala Ser Trp Ala
1 5 10 15
Lys Gly
<210> 203
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 203
Ala Ala Asn Thr Asp Thr Thr Tyr Phe Asn Leu
1 5 10
<210> 204
<211> 11
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 204
Gln Ala Ser Gln Ser Ile Asn Asn Gln Leu Ser
1 5 10
<210> 205
<211> 7
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 205
Gly Ala Ser Thr Leu Ala Ser
1 5
<210> 206
<211> 13
<212> PRT
<213> Artificial Sequence
<220>
<223> Synthesized
<400> 206
His Val His Tyr Cys Ser Gly Gly Ser Cys Phe Trp Ala
1 5 10

Claims (41)

1.一种双特异性抗体，其包括具有重链和轻链的IgG结构域，以及连接到所述重链的C端或所述轻链的N端的两个scFv部分，其中所述IgG结构域对第一抗原具有第一结合特异性，其中所述scFv部分对第二抗原具有第二结合特异性，并且其中所述第一抗原和所述第二抗原不同并且独立地选自α-CTLA4、α-PD-1和α-PD-L1。
2.根据权利要求1所述的双特异性抗体，其中，所述两个scFv部分连接到所述重链的所述C端。
3.根据权利要求2所述的双特异性抗体，其中，所述第一抗原包括α-CTLA4，所述第二抗原包括α-PD-1或α-PD-L1。
4.根据权利要求2所述的双特异性抗体，其中，所述第一抗原包括α-PD-1或α-PD-L1，所述第二抗原包括α-CTLA4。
5.根据权利要求1所述的双特异性抗体，其中，所述两个scFv部分连接到所述轻链的所述N端。
6.根据权利要求5所述的双特异性抗体，其中，所述第一抗原包括α-PD-1或α-PD-L1，所述第二抗原包括α-CTLA4。
7.根据权利要求5所述的双特异性抗体，其中，所述第一抗原包括α-CTLA4，所述第二抗原包括α-PD-1或α-PD-L1。
8.根据权利要求1所述的双特异性抗体，其中，所述双特异性抗体是分离的单克隆抗体。
9.根据权利要求1所述的双特异性抗体，其包括具有与SEQ ID No.92、94、96、98、100、102、104、106、108、110、112、114、116、118、120、122、124、126、128、130、132或134具有至少98％相似性的序列的抗原结合片段。
10.根据权利要求1所述的双特异性抗体，其具有对α-CTLA4的第一结合特异性和对α-PD-1或α-PD-L1的第二结合特异性，其中所述第一结合特异性和所述第二结合特异性都不具有大于70nM的Kd。
11.根据权利要求1所述的双特异性抗体，其具有对α-CTLA4的第一结合特异性和对α-PD-1的第二结合特异性，其中所述第一结合特异性和所述第二结合特异性都不具有大于70nM的Kd。
12.根据权利要求1所述的双特异性抗体，其具有对α-CTLA4的第一结合特异性和对α-PD-L1的第二结合特异性，其中所述第一结合特异性和所述第二结合特异性都不具有大于70nM的Kd。
13.根据权利要求1所述的双特异性抗体，其包括人框架区域。
14.根据权利要求1所述的双特异性抗体，其中，所述抗体是人源化抗体、嵌合抗体或重组抗体。
15.根据权利要求1所述的双特异性抗体，其中，所述IgG结构域包括IgG1恒定区，其中所述IgG1恒定区包括与SEQ ID No.136具有至少98％相似性的氨基酸序列。
16.一种IgG1重链，其包括与SEQ ID No.02、06、08、10、12、14、16、18、20、22、26、30、34、38、42、46、50、54、58、62、72、92、96、100、104,108、112、116、120、124、128或132具有至少98％相似性的氨基酸序列。
17.一种κ轻链，其包括与SEQ ID No.4、28、32、36、40、44、48、52、56、60和64具有至少98％相似性的氨基酸序列。
18.一种可变轻链，其包括与SEQ ID No.94、98、102、106、110、114、118、122、126、130或134具有至少98％相似性的氨基酸序列。
19.一种可变重链，其包括与SEQ ID No.92、96、100、104,108、112、116、120、124、128或132具有至少98％相似性的氨基酸序列。
20.一种分离的核酸，其编码根据权利要求1所述的双特异性抗体、根据权利要求16所述的IgG1重链、根据权利要求17所述的κ轻链、根据权利要求18所述的可变轻链或根据权利要求19所述的可变重链。
21.一种表达载体，其包括根据权利要求20所述的分离的核酸。
22.根据权利要求21所述的表达载体，其中，所述载体可在细胞中表达。
23.一种宿主细胞，其包括根据权利要求20所述的核酸。
24.一种宿主细胞，其包括根据权利要求21所述的表达载体。
25.根据权利要求23或24所述的宿主细胞，其中，所述宿主细胞是原核细胞或真核细胞。
26.一种产生双特异性抗体的方法，其包括培养根据权利要求23至25中的一个所述的宿主细胞，从而产生所述双特异性抗体。
27.一种免疫偶联物，其包括根据权利要求1所述的双特异性抗体以及细胞毒类药物。
28.根据权利要求27所述的免疫偶联物，其中，所述细胞毒类药物包括化疗药物、生长抑制剂、毒素或放射性同位素。
29.一种药物组合物，其包括根据权利要求1所述的双特异性抗体和药学上可接受的载体。
30.根据权利要求29所述的药物组合物，进一步包括放射性同位素、放射性核素、毒素、治疗剂、化疗药物或其组合。
31.一种药物组合物，其包括根据权利要求30所述的免疫偶联物和药学上可接受的载体。
32.一种对患有癌症的受试者进行治疗的方法，其包括施用所述受试者有效量的根据权利要求1所述的双特异性抗体。
33.根据权利要求32所述的方法，其中，所述癌症包括表达PD-L1的细胞。
34.根据权利要求32所述的方法，其中，所述癌症包括乳腺癌、结肠直肠癌、胰腺癌、头颈癌、黑素瘤、卵巢癌、前列腺癌、非小细胞肺癌、小细胞肺癌、神经胶质瘤、食管癌、鼻咽癌、肾癌、胃癌、肝癌、膀胱癌、宫颈癌、脑肿瘤、淋巴瘤、白血病、骨髓瘤。
35.根据权利要求32所述的方法，进一步包括共同施用有效量的治疗剂。
36.根据权利要求35所述的方法，其中，所述治疗剂包括抗体、化疗药物、酶或其组合。
37.根据权利要求36所述的方法，其中，所述治疗剂包括卡培他滨、顺铂、曲妥珠单抗、氟维司群、他莫昔芬、来曲唑、依西美坦、阿那曲唑、氨鲁米特、睾内酯、伏氯唑、福美坦、法曲唑、来曲唑、埃罗替尼、阿法替尼(lafatinib)、达沙替尼、吉非替尼、伊马替尼、帕唑替尼(pazopinib)、拉帕替尼、舒尼替尼、尼洛替尼、索拉非尼、nab-紫杉醇(nab-palitaxel)、其衍生物或组合。
38.根据权利要求32所述的方法，其中，所述受试者是人。
39.一种溶液，其包括有效浓度的根据权利要求1所述的双特异性抗体，其中所述溶液是受试者的血浆。
40.一种双特异性抗体，其具有对第一抗原的第一结合特异性和对第二抗原的第二结合特异性，其中所述第一抗原和所述第二抗原都是检查点抗原，并且其中所述第一特异性和所述第二特异性都不具有大于70nM的Kd。
41.一种双特异性抗体，其包括具有重链和轻链的IgG结构域，以及连接到所述重链的C端或所述轻链的N端的两个scFv部分，其中所述IgG结构域对第一抗原具有第一结合特异性，其中所述scFv部分对第二抗原具有第二结合特异性，并且其中所述第一抗原和所述第二抗原都是检查点抗原，并且其中所述第一抗原不同于所述第二抗原。
CN201880059130.9A 2017-11-02 2018-11-01 双特异性抗体及其制备和使用方法 Active CN111212658B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311257227.XA CN117343193A (zh) 2017-11-02 2018-11-01 双特异性抗体及其制备和使用方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762580845P 2017-11-02 2017-11-02
US62/580,845 2017-11-02
PCT/US2018/058810 WO2019090002A1 (en) 2017-11-02 2018-11-01 Bispecific antibodies and methods of making and using thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202311257227.XA Division CN117343193A (zh) 2017-11-02 2018-11-01 双特异性抗体及其制备和使用方法

Publications (2)

Publication Number Publication Date
CN111212658A true CN111212658A (zh) 2020-05-29
CN111212658B CN111212658B (zh) 2024-05-03

Family

ID=

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006105338A2 (en) * 2005-03-31 2006-10-05 Xencor, Inc. Fc VARIANTS WITH OPTIMIZED PROPERTIES
US20070281334A1 (en) * 2002-03-29 2007-12-06 Xoma Technology Ltd. Methods and materials for increasing expression of recombinant polypeptides
US20160145355A1 (en) * 2013-06-24 2016-05-26 Biomed Valley Discoveries, Inc. Bispecific antibodies
US20170073418A1 (en) * 2014-12-22 2017-03-16 Systimmune, Inc. Bispecific Tetravalent Antibodies and Methods of Making and Using Thereof
CN106967172A (zh) * 2016-08-23 2017-07-21 中山康方生物医药有限公司 抗ctla4‑抗pd‑1 双功能抗体、其药物组合物及其用途

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070281334A1 (en) * 2002-03-29 2007-12-06 Xoma Technology Ltd. Methods and materials for increasing expression of recombinant polypeptides
WO2006105338A2 (en) * 2005-03-31 2006-10-05 Xencor, Inc. Fc VARIANTS WITH OPTIMIZED PROPERTIES
US20160145355A1 (en) * 2013-06-24 2016-05-26 Biomed Valley Discoveries, Inc. Bispecific antibodies
US20170073418A1 (en) * 2014-12-22 2017-03-16 Systimmune, Inc. Bispecific Tetravalent Antibodies and Methods of Making and Using Thereof
CN107206074A (zh) * 2014-12-22 2017-09-26 西雅图免疫公司 双特异性四价抗体及其制备和使用方法
CN106967172A (zh) * 2016-08-23 2017-07-21 中山康方生物医药有限公司 抗ctla4‑抗pd‑1 双功能抗体、其药物组合物及其用途

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DANIELLE M LUSSIER等: "Combination immunotherapy with α-CTLA-4 and α-PD-L1 antibody blockade prevents immune escape and leads to complete control of metastatic osteosarcoma" *

Also Published As

Publication number Publication date
WO2019090002A1 (en) 2019-05-09
JP7418326B2 (ja) 2024-01-19
US20200347137A1 (en) 2020-11-05
RU2020108444A3 (zh) 2022-03-17
SG11202003237QA (en) 2020-05-28
AU2018358138A1 (en) 2020-04-23
AU2018358138B2 (en) 2022-06-02
AU2018358138C1 (en) 2022-12-08
CA3069238A1 (en) 2019-05-09
EP3703736A1 (en) 2020-09-09
IL271346A (en) 2020-01-30
EP3703736A4 (en) 2021-11-03
CN117343193A (zh) 2024-01-05
TW201927819A (zh) 2019-07-16
KR20200091382A (ko) 2020-07-30
RU2020108444A (ru) 2021-12-02
JP2021501575A (ja) 2021-01-21

Similar Documents

Publication Publication Date Title
CN110869389B (zh) 抗ror1抗体及其制备和使用方法
DK2519543T3 (en) HETERODIMER BINDING PROTEINS AND USE THEREOF
AU2024200534A1 (en) Anti-TL1A/anti-TNF-alpha bispecific antigen binding proteins and uses thereof
KR20210134300A (ko) 항-sars-cov-2 스파이크 당단백질 항체 및 항원-결합 단편
RU2753902C2 (ru) Комбинированная терапия на основе активирующих т-клетки биспецифических антигенсвязывающих молекул против cd3 и фолатного рецептора 1 (folr1) и антагонистов, связывающихся с осью pd-1
KR102230620B1 (ko) Ｃｄｈ19 및 ｃｄ3에 대한 항체 작제물
CN111954680B (zh) IL2Rβ/共同γ链抗体
KR20220167273A (ko) 항-코로나바이러스 항체 및 사용 방법
KR101459159B1 (ko) Ｏｘ-2/ｃｄ200에 대한 항체 및 이들의 용도
AU2021254602A1 (en) Humanized anti-MUC1* antibodies
AU2021203876A1 (en) Anti-CD3 antibodies, activatable anti-CD3 antibodies, multispecific anti-CD3 antibodies, multispecific activatable anti-CD3 antibodies, and methods of using the same
KR102551269B1 (ko) 저-점도 항원 결합 단백질 및 이의 제조 방법
KR102361237B1 (ko) 코일드 코일 면역글로불린 융합 단백질 및 이것의 조성물
KR20230002261A (ko) 항-sars-cov-2 중화 항체 및 이의 사용 방법
KR20150006471A (ko) 항-pcsk9 항체 및 그의 용도
KR20210030405A (ko) Ilt4에 결합하는 항체
KR20160021849A (ko) 렉틴-유사 산화된 ldl 수용체1 항체 및 사용 방법
KR20230017815A (ko) 항-sars-cov-2 스파이크 당단백질 항체 및 항원-결합 단편
KR20230027300A (ko) Il-10 뮤테인 및 이의 융합 단백질
KR20200091382A (ko) 이중특이성 항체 및 이의 제조 및 사용 방법
CN111212658B (zh) 双特异性抗体及其制备和使用方法
RU2787783C2 (ru) Биспецифические антитела и способы их получения и применения
KR20220154710A (ko) 소형 유도 및 항법 제어(miniGNC) 항체-유사 단백질 및 이의 제조 및 사용 방법
CN113316587B (zh) 一种双特异性分子及其制备与用途
CN115362175A (zh) 抗acvr1抗体及其用途

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40019735

Country of ref document: HK

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20220601

Address after: No. 15318, 95 Street northeast, Redmond, Washington, U. S.

Applicant after: Seattle Immunization Co.

Applicant after: Chengdu bailidote Biological Pharmaceutical Co.,Ltd.

Address before: No. 15318, 95 Street northeast, Redmond, Washington, U. S.

Applicant before: Seattle Immunization Co.

Applicant before: SICHUAN BAILI PHARM Co.,Ltd.

GR01 Patent grant