CN111201328B - 使用双半抗原探针的对重组酶聚合酶扩增的检测 - Google Patents

使用双半抗原探针的对重组酶聚合酶扩增的检测 Download PDF

Info

Publication number
CN111201328B
CN111201328B CN201880065797.XA CN201880065797A CN111201328B CN 111201328 B CN111201328 B CN 111201328B CN 201880065797 A CN201880065797 A CN 201880065797A CN 111201328 B CN111201328 B CN 111201328B
Authority
CN
China
Prior art keywords
nucleic acid
hapten
rpa
lateral flow
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201880065797.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN111201328A (zh
Inventor
M·L·鲍威尔
F·R·布乐尔
凯撒利尔·J·格林伍德
奥拉夫·派普拉
尼尔·A·阿姆斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Diagnostics Scarborough Inc
Original Assignee
Alere San Diego Inc
Abbott Diagnostics Scarborough Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alere San Diego Inc, Abbott Diagnostics Scarborough Inc filed Critical Alere San Diego Inc
Publication of CN111201328A publication Critical patent/CN111201328A/zh
Application granted granted Critical
Publication of CN111201328B publication Critical patent/CN111201328B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/11—Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
    • C12Y301/11002—Exodeoxyribonuclease III (3.1.11.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02023—DNA-formamidopyrimidine glycosylase (3.2.2.23)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201880065797.XA 2017-09-14 2018-09-14 使用双半抗原探针的对重组酶聚合酶扩增的检测 Active CN111201328B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762558705P 2017-09-14 2017-09-14
US62/558,705 2017-09-14
PCT/US2018/051078 WO2019055780A1 (en) 2017-09-14 2018-09-14 POLYMERASE RECOMBINASE AMPLIFICATION DETECTION USING DOUBLE HAPTEN PROBE

Publications (2)

Publication Number Publication Date
CN111201328A CN111201328A (zh) 2020-05-26
CN111201328B true CN111201328B (zh) 2024-07-02

Family

ID=65630678

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880065797.XA Active CN111201328B (zh) 2017-09-14 2018-09-14 使用双半抗原探针的对重组酶聚合酶扩增的检测

Country Status (12)

Country Link
US (1) US20190078147A1 (cg-RX-API-DMAC10.html)
EP (2) EP4464412A3 (cg-RX-API-DMAC10.html)
JP (2) JP7412332B2 (cg-RX-API-DMAC10.html)
KR (2) KR102800957B1 (cg-RX-API-DMAC10.html)
CN (1) CN111201328B (cg-RX-API-DMAC10.html)
AU (1) AU2018334226B2 (cg-RX-API-DMAC10.html)
BR (1) BR112020005121A2 (cg-RX-API-DMAC10.html)
CA (1) CA3075629A1 (cg-RX-API-DMAC10.html)
ES (1) ES2991912T3 (cg-RX-API-DMAC10.html)
MX (1) MX2020002826A (cg-RX-API-DMAC10.html)
RU (1) RU2020110056A (cg-RX-API-DMAC10.html)
WO (1) WO2019055780A1 (cg-RX-API-DMAC10.html)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102020103958B4 (de) * 2020-02-14 2021-08-26 Testo bioAnalytics GmbH Oligonukleotidsonde zum spezifischen Nachweis von Mikroorganismen, korrespondierendes Verfahren und Verwendung
EP3950955A1 (en) * 2020-08-06 2022-02-09 Albert-Ludwigs-Universität Freiburg Multianalyte assay for the simultaneous detection of nucleic acid and analytes
CN113552106B (zh) * 2021-07-23 2022-12-30 济南大学 一种检测ATP、谷胱甘肽、Fpg糖基化酶的通用荧光传感器
GR20220100698A (el) * 2022-08-18 2024-03-12 Πανεπιστημιο Πατρων, Μεθοδος βελτιωσης της ανιχνευσιμοτητας των δοκιμων που βασιζονται σε ταινιες ξηρων αντιδραστηριων
GB202500508D0 (en) 2025-01-15 2025-02-26 Biocrucible Ltd Molecular diagnostic device

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010135310A1 (en) * 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591552A (en) * 1982-09-29 1986-05-27 New York Blood Center, Inc. Detection of hepatitis B surface antigen (or antibody to same) with labeled synthetic peptide
PT1024191E (pt) * 1991-12-02 2008-12-22 Medical Res Council Produção de auto-anticorpos a partir de reportórios de segmentos de anticorpo e exibidos em fagos
US5770563A (en) * 1991-12-06 1998-06-23 The United States Of America As Represented By The Department Of Health And Human Services Heparin- and sulfatide binding peptides from the type I repeats of human thrombospondin and conjugates thereof
AU692152B2 (en) * 1993-03-17 1998-06-04 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Immunogenic chimeras comprising nucleic acid sequences encoding endoplasmic reticulum signal sequence peptides and at least one other peptide, and their uses in vaccines and disease treatments
DE4441626A1 (de) * 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
WO2002009738A1 (en) * 2000-07-28 2002-02-07 Murphy Christopher J Transplant media
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
US8030000B2 (en) * 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
EP2029782B1 (en) 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
US9334531B2 (en) * 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2011038197A1 (en) * 2009-09-25 2011-03-31 Alere San Diego, Inc. Detection of nucleic acids in crude matrices
ES2873850T3 (es) * 2013-08-19 2021-11-04 Abbott Molecular Inc Bibliotecas de secuenciación de próxima generación
WO2016168490A1 (en) * 2015-04-15 2016-10-20 University Of Washington Isotachophoresis enhanced isothermal nucleic acid amplification
GB201519565D0 (en) * 2015-11-05 2015-12-23 Alere San Diego Inc Sample preparation device
EP3368654B1 (en) * 2015-10-30 2025-11-19 Abbott Diagnostics Scarborough, Inc. Determination of polymorphisms using isothermal nucleic acid amplification
CN108463560B (zh) * 2016-01-15 2022-09-20 昆塔波尔公司 具有降低的背景的基于光学的纳米孔分析

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010135310A1 (en) * 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A multifunctional single-attachment-point reagent for controlled protein biotinylation;Elisabeth Garanger等;《Bioconjug Chem.》;第20卷(第1期);摘要、图2 *
Attaching Analytes in the Proximity of the Active Site of Enzymes;Alynne 1. MacLean等;《Journal of the Chemical Society Chemical Communications》(第24期);摘要、图1 *
Elisabeth Garanger等.A multifunctional single-attachment-point reagent for controlled protein biotinylation.《Bioconjug Chem.》.2009,第20卷(第1期),摘要、图2. *

Also Published As

Publication number Publication date
RU2020110056A (ru) 2021-10-14
JP2020537499A (ja) 2020-12-24
JP2024045115A (ja) 2024-04-02
KR20250058779A (ko) 2025-04-30
JP7884496B2 (ja) 2026-07-03
AU2018334226B2 (en) 2024-07-11
EP3682029A4 (en) 2021-06-02
JP7412332B2 (ja) 2024-01-12
EP3682029A1 (en) 2020-07-22
MX2020002826A (es) 2020-07-22
KR102800957B1 (ko) 2025-04-30
WO2019055780A1 (en) 2019-03-21
BR112020005121A2 (pt) 2020-09-24
CN111201328A (zh) 2020-05-26
CA3075629A1 (en) 2019-03-21
US20190078147A1 (en) 2019-03-14
ES2991912T3 (es) 2024-12-05
EP3682029B1 (en) 2024-08-14
KR20200054268A (ko) 2020-05-19
EP4464412A2 (en) 2024-11-20
EP4464412A3 (en) 2025-02-26
AU2018334226A1 (en) 2020-04-02

Similar Documents

Publication Publication Date Title
US12281355B2 (en) Multiplexed imaging with enzyme mediated amplification
US11639518B2 (en) Compositions and methods for analyte detection
KR102800957B1 (ko) 이중-합텐 프로브를 이용한 재조합효소 폴리머라제 증폭의 검출
CN103703145B (zh) 解折叠邻近探针及其使用方法
AU2019280824A1 (en) Multiplexed catalyzed reporter deposition
CN101836117B (zh) 用于检测生物靶点的快速和灵敏的方法
US11873312B2 (en) Transcriptome in vivo analysis (TIVA) and transcriptome in situ analysis (TISA)
JPH05508074A (ja) インビトロにおける増幅を用いたポリヌクレオチド捕捉アッセイ
JP2005519627A (ja) バイオコンジュゲート‐ナノ粒子プローブ
WO2000056920A1 (en) One step sample preparation and detection of nucleic acids in complex biological samples
JP7046082B2 (ja) 新規化合物及びサンプル中の標的分子の検出のためのその使用
CA2902982A1 (en) Polymethine compounds and their use as fluorescent labels
WO2020176534A1 (en) Multiplexed signal amplification methods using enzymatic based chemical deposition
CN114829368A (zh) 通过使用连续标记方案的合成进行测序的方法
HK40119331A (en) Detection of recombinase polymerase amplification using dual-hapten probe
KR20010042167A (ko) 검출 복합체에 관한 방법, 키트 및 조성물
BR122025013616A2 (pt) Composição de amplificação pela polimerase recombinase, dispositivo e método de detecção de produtos de amplificação
US20180119149A1 (en) In vitro selection of formaldehyde cross-linking aptamers
WO2024224323A1 (en) Compositions, systems and methods for multiplex detection of target biomarkers in a sample
US20240010685A1 (en) Rna sensors and uses thereof
KR20160134094A (ko) 펩티드핵산을 이용한 항체 의존성 세포 독성에 관여하는 항체 Fc 감마 리셉터의 유전자형 판별방법

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20201030

Address after: Maine

Applicant after: Abbott diagnostic products Scarborough

Address before: California, USA

Applicant before: Alere San Diego Inc.

TA01 Transfer of patent application right
GR01 Patent grant