RU2020110056A - Обнаружение рекомбиназной полимеразной амплификации с применением зонда с двойным гаптеном - Google Patents

Обнаружение рекомбиназной полимеразной амплификации с применением зонда с двойным гаптеном Download PDF

Info

Publication number
RU2020110056A
RU2020110056A RU2020110056A RU2020110056A RU2020110056A RU 2020110056 A RU2020110056 A RU 2020110056A RU 2020110056 A RU2020110056 A RU 2020110056A RU 2020110056 A RU2020110056 A RU 2020110056A RU 2020110056 A RU2020110056 A RU 2020110056A
Authority
RU
Russia
Prior art keywords
kda
nucleic acid
hapten
zone
composition
Prior art date
Application number
RU2020110056A
Other languages
English (en)
Russian (ru)
Inventor
Майкл Л. ПАУЭЛЛ
Фрэнк Рэй БОУЛЕР
Кэтрин Джин ГРИНВУД
Олаф ПИПЕНБУРГ
Найл А. АРМС
Original Assignee
Эбботт Диагностикс Скарборо, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Эбботт Диагностикс Скарборо, Инк. filed Critical Эбботт Диагностикс Скарборо, Инк.
Publication of RU2020110056A publication Critical patent/RU2020110056A/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/11—Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
    • C12Y301/11002—Exodeoxyribonuclease III (3.1.11.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02023—DNA-formamidopyrimidine glycosylase (3.2.2.23)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
RU2020110056A 2017-09-14 2018-09-14 Обнаружение рекомбиназной полимеразной амплификации с применением зонда с двойным гаптеном RU2020110056A (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762558705P 2017-09-14 2017-09-14
US62/558,705 2017-09-14
PCT/US2018/051078 WO2019055780A1 (en) 2017-09-14 2018-09-14 POLYMERASE RECOMBINASE AMPLIFICATION DETECTION USING DOUBLE HAPTEN PROBE

Publications (1)

Publication Number Publication Date
RU2020110056A true RU2020110056A (ru) 2021-10-14

Family

ID=65630678

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2020110056A RU2020110056A (ru) 2017-09-14 2018-09-14 Обнаружение рекомбиназной полимеразной амплификации с применением зонда с двойным гаптеном

Country Status (12)

Country Link
US (1) US20190078147A1 (cg-RX-API-DMAC10.html)
EP (2) EP4464412A3 (cg-RX-API-DMAC10.html)
JP (2) JP7412332B2 (cg-RX-API-DMAC10.html)
KR (2) KR102800957B1 (cg-RX-API-DMAC10.html)
CN (1) CN111201328B (cg-RX-API-DMAC10.html)
AU (1) AU2018334226B2 (cg-RX-API-DMAC10.html)
BR (1) BR112020005121A2 (cg-RX-API-DMAC10.html)
CA (1) CA3075629A1 (cg-RX-API-DMAC10.html)
ES (1) ES2991912T3 (cg-RX-API-DMAC10.html)
MX (1) MX2020002826A (cg-RX-API-DMAC10.html)
RU (1) RU2020110056A (cg-RX-API-DMAC10.html)
WO (1) WO2019055780A1 (cg-RX-API-DMAC10.html)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102020103958B4 (de) * 2020-02-14 2021-08-26 Testo bioAnalytics GmbH Oligonukleotidsonde zum spezifischen Nachweis von Mikroorganismen, korrespondierendes Verfahren und Verwendung
EP3950955A1 (en) * 2020-08-06 2022-02-09 Albert-Ludwigs-Universität Freiburg Multianalyte assay for the simultaneous detection of nucleic acid and analytes
CN113552106B (zh) * 2021-07-23 2022-12-30 济南大学 一种检测ATP、谷胱甘肽、Fpg糖基化酶的通用荧光传感器
GR20220100698A (el) * 2022-08-18 2024-03-12 Πανεπιστημιο Πατρων, Μεθοδος βελτιωσης της ανιχνευσιμοτητας των δοκιμων που βασιζονται σε ταινιες ξηρων αντιδραστηριων
GB202500508D0 (en) 2025-01-15 2025-02-26 Biocrucible Ltd Molecular diagnostic device

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591552A (en) * 1982-09-29 1986-05-27 New York Blood Center, Inc. Detection of hepatitis B surface antigen (or antibody to same) with labeled synthetic peptide
PT1024191E (pt) * 1991-12-02 2008-12-22 Medical Res Council Produção de auto-anticorpos a partir de reportórios de segmentos de anticorpo e exibidos em fagos
US5770563A (en) * 1991-12-06 1998-06-23 The United States Of America As Represented By The Department Of Health And Human Services Heparin- and sulfatide binding peptides from the type I repeats of human thrombospondin and conjugates thereof
AU692152B2 (en) * 1993-03-17 1998-06-04 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Immunogenic chimeras comprising nucleic acid sequences encoding endoplasmic reticulum signal sequence peptides and at least one other peptide, and their uses in vaccines and disease treatments
DE4441626A1 (de) * 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
WO2002009738A1 (en) * 2000-07-28 2002-02-07 Murphy Christopher J Transplant media
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
US8030000B2 (en) * 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
EP2029782B1 (en) 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
US9334531B2 (en) * 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
JP5847076B2 (ja) * 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2011038197A1 (en) * 2009-09-25 2011-03-31 Alere San Diego, Inc. Detection of nucleic acids in crude matrices
ES2873850T3 (es) * 2013-08-19 2021-11-04 Abbott Molecular Inc Bibliotecas de secuenciación de próxima generación
WO2016168490A1 (en) * 2015-04-15 2016-10-20 University Of Washington Isotachophoresis enhanced isothermal nucleic acid amplification
GB201519565D0 (en) * 2015-11-05 2015-12-23 Alere San Diego Inc Sample preparation device
EP3368654B1 (en) * 2015-10-30 2025-11-19 Abbott Diagnostics Scarborough, Inc. Determination of polymorphisms using isothermal nucleic acid amplification
CN108463560B (zh) * 2016-01-15 2022-09-20 昆塔波尔公司 具有降低的背景的基于光学的纳米孔分析

Also Published As

Publication number Publication date
JP2020537499A (ja) 2020-12-24
JP2024045115A (ja) 2024-04-02
KR20250058779A (ko) 2025-04-30
JP7884496B2 (ja) 2026-07-03
AU2018334226B2 (en) 2024-07-11
EP3682029A4 (en) 2021-06-02
JP7412332B2 (ja) 2024-01-12
EP3682029A1 (en) 2020-07-22
MX2020002826A (es) 2020-07-22
CN111201328B (zh) 2024-07-02
KR102800957B1 (ko) 2025-04-30
WO2019055780A1 (en) 2019-03-21
BR112020005121A2 (pt) 2020-09-24
CN111201328A (zh) 2020-05-26
CA3075629A1 (en) 2019-03-21
US20190078147A1 (en) 2019-03-14
ES2991912T3 (es) 2024-12-05
EP3682029B1 (en) 2024-08-14
KR20200054268A (ko) 2020-05-19
EP4464412A2 (en) 2024-11-20
EP4464412A3 (en) 2025-02-26
AU2018334226A1 (en) 2020-04-02

Similar Documents

Publication Publication Date Title
JP2022522480A5 (cg-RX-API-DMAC10.html)
CN103703145B (zh) 解折叠邻近探针及其使用方法
US7122319B2 (en) Multiplexed immunohistochemical assays using molecular tags
JP2020537499A5 (cg-RX-API-DMAC10.html)
KR101719285B1 (ko) 표적 물질에 의해 조절되는 핵산 중합효소 활성을 이용한 생체물질의 검출 및 정량 방법
US8741558B2 (en) Compositions, methods, and kits for identifying candidate molecules from encoded chemical libraries
EP3277834B1 (en) Methods of capturing sperm nucleic acids
CN106544424A (zh) 多交叉恒温扩增结合金纳米生物传感检测志贺菌的方法
WO2019084458A1 (en) HIGHLY SPECIFIC DOSINGS
WO2010056579A1 (en) Methods and compositions in particle-based detection of target molecules using covalent bond forming reactive pairs
CN104404162A (zh) 采用引物关联通用探针检测多重基因或不同靶标的实时荧光pcr方法
US11293055B2 (en) Nucleic acid detection kit and nucleic acid detection method using nanoparticles
US11692215B2 (en) Nucleic acid cleaving enzyme-based biosensor and methods of use thereof
CN107523624B (zh) 一种结合audg和自避分子识别系统的多交叉恒温扩增方法
Xiang et al. Graphene oxide and molecular beacons-based multiplexed DNA detection by synchronous fluorescence analysis
JP2016214101A (ja) 口腔細菌の簡易迅速検査方法
KR20200095870A (ko) 압타머를 이용한 다중(multiplex) PCR 방법
KR102852958B1 (ko) 표적 rna 분자 및 표적 단백질을 동시에 검출하는 방법 및 키트
WO2021215989A1 (en) Rapid detection of specific genetic sequences using a multi-labelled dna hybrid comprising a reporter strand and an anchor strand
CN119365595A (zh) 纯化抗体-核酸缀合物的方法
EP2202319A1 (en) Nucleic acid probe, and method for production of probe polymer
US7118864B2 (en) Amplifiable probe
US9890418B1 (en) Method of detecting nucleic acids with enhanced signal using dual-functional capture particles
WO2007108378A1 (ja) シグナルプローブポリマーの形成方法
US20180148772A1 (en) Method for Quantitatively Profiling Nucleic Acids

Legal Events

Date Code Title Description
FA93 Acknowledgement of application withdrawn (no request for examination)

Effective date: 20210915