CN111073848A - 一种卵裂期胚胎培养组合物及卵裂期胚胎培养液 - Google Patents
一种卵裂期胚胎培养组合物及卵裂期胚胎培养液 Download PDFInfo
- Publication number
- CN111073848A CN111073848A CN202010003060.4A CN202010003060A CN111073848A CN 111073848 A CN111073848 A CN 111073848A CN 202010003060 A CN202010003060 A CN 202010003060A CN 111073848 A CN111073848 A CN 111073848A
- Authority
- CN
- China
- Prior art keywords
- embryo culture
- cleavage stage
- stage embryo
- cleavage
- sodium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 106
- 238000003776 cleavage reaction Methods 0.000 title claims abstract description 97
- 230000007017 scission Effects 0.000 title claims abstract description 97
- 239000000203 mixture Substances 0.000 title claims abstract description 35
- 102000008100 Human Serum Albumin Human genes 0.000 claims abstract description 21
- 108091006905 Human Serum Albumin Proteins 0.000 claims abstract description 21
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229960003987 melatonin Drugs 0.000 claims abstract description 21
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000005515 coenzyme Substances 0.000 claims abstract description 5
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims description 22
- 239000012531 culture fluid Substances 0.000 claims description 21
- 235000017471 coenzyme Q10 Nutrition 0.000 claims description 20
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 claims description 20
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims description 19
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 18
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 18
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 12
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 12
- 229940024606 amino acid Drugs 0.000 claims description 12
- 235000001014 amino acid Nutrition 0.000 claims description 12
- 150000001413 amino acids Chemical class 0.000 claims description 12
- HJCMDXDYPOUFDY-WHFBIAKZSA-N Ala-Gln Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O HJCMDXDYPOUFDY-WHFBIAKZSA-N 0.000 claims description 11
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 11
- 229960002648 alanylglutamine Drugs 0.000 claims description 11
- 108010044940 alanylglutamine Proteins 0.000 claims description 11
- 239000008103 glucose Substances 0.000 claims description 11
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 11
- 229960003080 taurine Drugs 0.000 claims description 11
- 150000000000 tetracarboxylic acids Chemical class 0.000 claims description 11
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 10
- 235000002639 sodium chloride Nutrition 0.000 claims description 10
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 9
- 239000004471 Glycine Substances 0.000 claims description 9
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 9
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 9
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims description 9
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 9
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 9
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 9
- 235000004279 alanine Nutrition 0.000 claims description 9
- 235000009582 asparagine Nutrition 0.000 claims description 9
- 229960001230 asparagine Drugs 0.000 claims description 9
- 239000001110 calcium chloride Substances 0.000 claims description 9
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 claims description 8
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 8
- 229960001031 glucose Drugs 0.000 claims description 8
- 229940005581 sodium lactate Drugs 0.000 claims description 8
- 239000001540 sodium lactate Substances 0.000 claims description 8
- 235000011088 sodium lactate Nutrition 0.000 claims description 8
- 229940054269 sodium pyruvate Drugs 0.000 claims description 8
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims description 6
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 6
- 235000003704 aspartic acid Nutrition 0.000 claims description 6
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 6
- 235000011148 calcium chloride Nutrition 0.000 claims description 6
- 235000013922 glutamic acid Nutrition 0.000 claims description 6
- 239000004220 glutamic acid Substances 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 6
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 6
- 239000001103 potassium chloride Substances 0.000 claims description 6
- 235000011164 potassium chloride Nutrition 0.000 claims description 6
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 6
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims 4
- 102000048143 Insulin-Like Growth Factor II Human genes 0.000 claims 3
- 108090001117 Insulin-Like Growth Factor II Proteins 0.000 claims 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 15
- 230000001737 promoting effect Effects 0.000 abstract description 8
- 230000011278 mitosis Effects 0.000 abstract description 7
- 230000011712 cell development Effects 0.000 abstract description 5
- 230000000052 comparative effect Effects 0.000 description 19
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 16
- 101001076292 Homo sapiens Insulin-like growth factor II Proteins 0.000 description 16
- 102100025947 Insulin-like growth factor II Human genes 0.000 description 16
- 229940110767 coenzyme Q10 Drugs 0.000 description 16
- 229940068935 insulin-like growth factor 2 Drugs 0.000 description 16
- 210000002257 embryonic structure Anatomy 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 13
- 238000000338 in vitro Methods 0.000 description 11
- 229930182566 Gentamicin Natural products 0.000 description 8
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 8
- 238000011161 development Methods 0.000 description 8
- 230000018109 developmental process Effects 0.000 description 8
- 229960002518 gentamicin Drugs 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 229960003767 alanine Drugs 0.000 description 7
- 230000000844 anti-bacterial effect Effects 0.000 description 7
- 229960005261 aspartic acid Drugs 0.000 description 7
- 239000003899 bactericide agent Substances 0.000 description 7
- 229960001484 edetic acid Drugs 0.000 description 7
- 229960002989 glutamic acid Drugs 0.000 description 7
- 229960002429 proline Drugs 0.000 description 7
- 229960001153 serine Drugs 0.000 description 7
- 238000012258 culturing Methods 0.000 description 6
- 230000004720 fertilization Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 210000003470 mitochondria Anatomy 0.000 description 6
- 239000003642 reactive oxygen metabolite Substances 0.000 description 6
- 239000003963 antioxidant agent Substances 0.000 description 5
- 230000003078 antioxidant effect Effects 0.000 description 5
- 235000013601 eggs Nutrition 0.000 description 5
- 210000000287 oocyte Anatomy 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 241000282414 Homo sapiens Species 0.000 description 4
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 4
- 235000006708 antioxidants Nutrition 0.000 description 4
- 210000001109 blastomere Anatomy 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 229960002449 glycine Drugs 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 230000002000 scavenging effect Effects 0.000 description 4
- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 3
- CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000007640 basal medium Substances 0.000 description 3
- 230000013020 embryo development Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 230000037361 pathway Effects 0.000 description 3
- 108020003175 receptors Proteins 0.000 description 3
- 102000005962 receptors Human genes 0.000 description 3
- 229910000162 sodium phosphate Inorganic materials 0.000 description 3
- NGSFWBMYFKHRBD-DKWTVANSSA-M sodium;(2s)-2-hydroxypropanoate Chemical compound [Na+].C[C@H](O)C([O-])=O NGSFWBMYFKHRBD-DKWTVANSSA-M 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- BKOOMYPCSUNDGP-UHFFFAOYSA-N 2-methylbut-2-ene Chemical group CC=C(C)C BKOOMYPCSUNDGP-UHFFFAOYSA-N 0.000 description 2
- 239000004599 antimicrobial Substances 0.000 description 2
- 210000002459 blastocyst Anatomy 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 210000003463 organelle Anatomy 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 230000002407 ATP formation Effects 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 108091006149 Electron carriers Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 102100037852 Insulin-like growth factor I Human genes 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 102000001419 Melatonin receptor Human genes 0.000 description 1
- 108050009605 Melatonin receptor Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 102000015494 Mitochondrial Uncoupling Proteins Human genes 0.000 description 1
- 108010050258 Mitochondrial Uncoupling Proteins Proteins 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000625 blastula Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000000091 immunopotentiator Effects 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 210000003563 lymphoid tissue Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 230000004898 mitochondrial function Effects 0.000 description 1
- 230000008811 mitochondrial respiratory chain Effects 0.000 description 1
- 230000002297 mitogenic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000003101 oviduct Anatomy 0.000 description 1
- 230000010627 oxidative phosphorylation Effects 0.000 description 1
- AZQWKYJCGOJGHM-UHFFFAOYSA-N para-benzoquinone Natural products O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 230000009993 protective function Effects 0.000 description 1
- -1 quinone compound Chemical class 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 210000005000 reproductive tract Anatomy 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0604—Whole embryos; Culture medium therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Gynecology & Obstetrics (AREA)
- Biotechnology (AREA)
- Reproductive Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
物料 | 浓度 |
NaCl | 90.08mmol/L |
KC1 | 5.5mmol/L |
NaH<sub>2</sub>P0<sub>4</sub> | 0.25mmol/L |
MgSO<sub>4</sub> | 1mmol/L |
NaHCO<sub>3</sub> | 25mmol/L |
CaCl<sub>2</sub> | 1.8mmol/L |
葡萄糖 | 0.5mmol/L |
乳酸钠 | 10.5mmol/L |
丙酮酸钠 | 0.32mmol/L |
丙氨酸 | 0.1mmol/L |
天门冬氨酸 | 0.1mmol/L |
天冬酰胺 | 0.1mmol/L |
谷氨酸 | 0.1mmol/L |
甘氨酸 | 0.1mmol/L |
脯氨酸 | 0.1mmol/L |
丝氨酸 | 0.1mmol/L |
丙氨酰-谷氨酰胺 | 1mmol/L |
牛磺酸 | 0.1mmol/L |
四元羧酸乙二胺四乙酸 | 0.01mmol/L |
HSA | 5g/L |
庆大霉素 | 50mg/L |
辅酶Q10 | 1mg/L |
褪黑素 | 1nmol/L |
IGF-2 | 50nmol/L |
物料 | 浓度 |
NaCl | 90.08mmol/L |
KC1 | 5.5mmol/L |
NaH<sub>2</sub>P0<sub>4</sub> | 0.25mmol/L |
MgSO<sub>4</sub> | 1mmol/L |
NaHCO<sub>3</sub> | 25mmol/L |
CaCl<sub>2</sub> | 1.8mmol/L |
葡萄糖 | 0.5mmol/L |
乳酸钠 | 10.5mmol/L |
丙酮酸钠 | 0.32mmol/L |
丙氨酸 | 0.1mmol/L |
天门冬氨酸 | 0.1mmol/L |
天冬酰胺 | 0.1mmol/L |
谷氨酸 | 0.1mmol/L |
甘氨酸 | 0.1mmol/L |
脯氨酸 | 0.1mmol/L |
丝氨酸 | 0.1mmol/L |
丙氨酰-谷氨酰胺 | 1mmol/L |
牛磺酸 | 0.1mmol/L |
四元羧酸乙二胺四乙酸 | 0.01mmol/L |
HSA | 5g/L |
庆大霉素 | 50mg/L |
辅酶Q10 | 1mg/L |
IGF-2 | 50nmol/L |
物料 | 浓度 |
NaCl | 90.08mmol/L |
KC1 | 5.5mmol/L |
NaH<sub>2</sub>P0<sub>4</sub> | 0.25mmol/L |
MgSO<sub>4</sub> | 1mmol/L |
NaHCO<sub>3</sub> | 25mmol/L |
CaCl<sub>2</sub> | 1.8mmol/L |
葡萄糖 | 0.5mmol/L |
乳酸钠 | 10.5mmol/L |
丙酮酸钠 | 0.32mmol/L |
丙氨酸 | 0.1mmol/L |
天门冬氨酸 | 0.1mmol/L |
天冬酰胺 | 0.1mmol/L |
谷氨酸 | 0.1mmol/L |
甘氨酸 | 0.1mmol/L |
脯氨酸 | 0.1mmol/L |
丝氨酸 | 0.1mmol/L |
丙氨酰-谷氨酰胺 | 1mmol/L |
牛磺酸 | 0.1mmol/L |
四元羧酸乙二胺四乙酸 | 0.01mmol/L |
HSA | 5g/L |
庆大霉素 | 50mg/L |
辅酶Q10 | 1mg/L |
褪黑素 | 1nmol/L |
物料 | 浓度 |
G-1.5 | -- |
HSA | 5g/L |
辅酶Q10 | 1mg/L |
褪黑素 | 1nmol/L |
IGF-2 | 50nmol/L |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010003060.4A CN111073848B (zh) | 2020-01-02 | 2020-01-02 | 一种卵裂期胚胎培养组合物及卵裂期胚胎培养液 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010003060.4A CN111073848B (zh) | 2020-01-02 | 2020-01-02 | 一种卵裂期胚胎培养组合物及卵裂期胚胎培养液 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111073848A true CN111073848A (zh) | 2020-04-28 |
CN111073848B CN111073848B (zh) | 2023-07-07 |
Family
ID=70321736
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010003060.4A Active CN111073848B (zh) | 2020-01-02 | 2020-01-02 | 一种卵裂期胚胎培养组合物及卵裂期胚胎培养液 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111073848B (zh) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113005076A (zh) * | 2021-05-07 | 2021-06-22 | 江苏省人民医院 | 未成熟卵母细胞体外培养液及其应用 |
CN113755429A (zh) * | 2021-09-29 | 2021-12-07 | 中国科学院苏州生物医学工程技术研究所 | 用于制备卵裂培养液的组合物、卵裂培养液及制备方法 |
CN113834936A (zh) * | 2021-08-20 | 2021-12-24 | 李竞宇 | 生长分化因子9在预测胚胎发育潜力中的应用 |
EP3957719A1 (en) * | 2020-08-21 | 2022-02-23 | Shenzhen Hongji Biotechnology Co., Ltd | Modified mesenchymal stem cell culture medium, bone marrow mesenchymal stem cells and culture methods and use thereof |
CN116814525A (zh) * | 2023-06-09 | 2023-09-29 | 广东海洋大学 | 一种用于东风螺胚胎培养的培养基及东风螺胚胎囊外培养的方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102703377A (zh) * | 2012-06-08 | 2012-10-03 | 中国农业大学 | 一种提高动物体外胚胎囊胚发育率的方法 |
US20140315995A1 (en) * | 2013-04-22 | 2014-10-23 | Neocutis Sa | Antioxidant Compositions and Methods of Using the Same |
CN108251355A (zh) * | 2018-01-31 | 2018-07-06 | 成都艾伟孚生物科技有限公司 | 一步式胚胎培养液 |
CN108251354A (zh) * | 2018-01-31 | 2018-07-06 | 成都艾伟孚生物科技有限公司 | 一步式胚胎培养液及其制备方法 |
WO2018189436A1 (fr) * | 2017-04-12 | 2018-10-18 | Patrick Choay Sas | Adjuvants dépourvus de cytokines pour milieux de culture cellulaire notamment pour fécondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons |
CN108728403A (zh) * | 2018-06-05 | 2018-11-02 | 瑞柏生物(中国)股份有限公司 | 一种卵裂培养液及其制备方法 |
-
2020
- 2020-01-02 CN CN202010003060.4A patent/CN111073848B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102703377A (zh) * | 2012-06-08 | 2012-10-03 | 中国农业大学 | 一种提高动物体外胚胎囊胚发育率的方法 |
US20140315995A1 (en) * | 2013-04-22 | 2014-10-23 | Neocutis Sa | Antioxidant Compositions and Methods of Using the Same |
CN105120834A (zh) * | 2013-04-22 | 2015-12-02 | 新科蒂斯公司 | 抗氧化剂组合物及其使用方法 |
WO2018189436A1 (fr) * | 2017-04-12 | 2018-10-18 | Patrick Choay Sas | Adjuvants dépourvus de cytokines pour milieux de culture cellulaire notamment pour fécondation in vitro, ou pour la culture de follicules, cellules germinales males ou embryons |
CN108251355A (zh) * | 2018-01-31 | 2018-07-06 | 成都艾伟孚生物科技有限公司 | 一步式胚胎培养液 |
CN108251354A (zh) * | 2018-01-31 | 2018-07-06 | 成都艾伟孚生物科技有限公司 | 一步式胚胎培养液及其制备方法 |
CN108728403A (zh) * | 2018-06-05 | 2018-11-02 | 瑞柏生物(中国)股份有限公司 | 一种卵裂培养液及其制备方法 |
Non-Patent Citations (8)
Title |
---|
M STOJKOVIC等: "Coenzyme Q10 in submicron-sized dispersion improves development, hatching, cell proliferation, and adenosine triphosphate content of in vitro-produced bovine embryos", 《BIOL REPROD》 * |
M STOJKOVIC等: "Coenzyme Q10 in submicron-sized dispersion improves development, hatching, cell proliferation, and adenosine triphosphate content of in vitro-produced bovine embryos", 《BIOL REPROD》, vol. 61, no. 2, 31 August 1999 (1999-08-31), pages 541 - 547, XP055057685, DOI: 10.1095/biolreprod61.2.541 * |
MORTEZAEE K 等: "Therapeutic value of melatonin post-treatment on CCl4-induced fibrotic rat liver", 《 CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY》 * |
MORTEZAEE K 等: "Therapeutic value of melatonin post-treatment on CCl4-induced fibrotic rat liver", 《 CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY》, vol. 94, no. 2, 31 December 2016 (2016-12-31), pages 119 - 130 * |
李建娟 等: "胚胎培养液中 IGF-Ⅱ与胚胎的关系", 《潍坊医学院学报》 * |
李建娟 等: "胚胎培养液中 IGF-Ⅱ与胚胎的关系", 《潍坊医学院学报》, vol. 2014, no. 3, 12 June 2014 (2014-06-12), pages 222 * |
胡晓东 等: "褪黑素对人卵裂期胚胎发育的影响及机制研究", 《生殖医学杂志》 * |
胡晓东 等: "褪黑素对人卵裂期胚胎发育的影响及机制研究", 《生殖医学杂志》, vol. 25, no. 8, 15 August 2016 (2016-08-15) * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3957719A1 (en) * | 2020-08-21 | 2022-02-23 | Shenzhen Hongji Biotechnology Co., Ltd | Modified mesenchymal stem cell culture medium, bone marrow mesenchymal stem cells and culture methods and use thereof |
CN113005076A (zh) * | 2021-05-07 | 2021-06-22 | 江苏省人民医院 | 未成熟卵母细胞体外培养液及其应用 |
CN113834936A (zh) * | 2021-08-20 | 2021-12-24 | 李竞宇 | 生长分化因子9在预测胚胎发育潜力中的应用 |
CN113755429A (zh) * | 2021-09-29 | 2021-12-07 | 中国科学院苏州生物医学工程技术研究所 | 用于制备卵裂培养液的组合物、卵裂培养液及制备方法 |
CN116814525A (zh) * | 2023-06-09 | 2023-09-29 | 广东海洋大学 | 一种用于东风螺胚胎培养的培养基及东风螺胚胎囊外培养的方法 |
CN116814525B (zh) * | 2023-06-09 | 2024-04-30 | 广东海洋大学 | 一种用于东风螺胚胎培养的培养基及东风螺胚胎囊外培养的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN111073848B (zh) | 2023-07-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111073848A (zh) | 一种卵裂期胚胎培养组合物及卵裂期胚胎培养液 | |
Wiley et al. | Effect of potassium concentration, type of protein supplement, and embryo density on mouse preimplantation development in vitro | |
EP0872180B1 (en) | Medium composition for external fertilization | |
JP4202121B2 (ja) | 哺乳動物の配偶子および胚の培養基サプリメント、およびその使用法 | |
Kane | A review of in vitro gamete maturation and embryo culture and potential impact on future animal biotechnology | |
US6610543B2 (en) | Cellular culture medium, particularly for in vitro fertilization, or for the culture of follicles, male germ cells or embryos | |
CN110923199B (zh) | 一种卵细胞激活培养液及其使用方法 | |
Loutradis et al. | Biological factors in culture media affecting in vitro fertilization, preimplantation embryo development, and implantation | |
Brinster | Culture of two-cell rabbit embryos to morulae | |
Palasz et al. | The effect of macromolecular supplementation on the surface tension of TCM-199 and the utilization of growth factors by bovine oocytes and embryos in culture | |
CN112048467A (zh) | Ksom-aa培养液在体外培养nod背景鼠胚胎中的应用 | |
Feng et al. | Fertilization and early embryology: Effect of different co-culture systems in early human embryo development | |
Pool | Development of culture media for human assisted reproductive technology | |
Chida | Monozygous double inner cell masses in mouse blastocysts following fertilization in vitro and in vivo | |
Salvador et al. | Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine | |
Hoppe et al. | An albumin requirement for fertilization of mouse eggs in vitro | |
CN110452868A (zh) | 一种一步式胚胎培养液 | |
EP2740790B1 (en) | Composition for embryo culture | |
Spindle | In vitro development of one-cell embryos from outbred mice: influence of culture medium composition | |
CN101886059A (zh) | 用于胚胎体外生产的培养液以及牛胚胎体外生产的方法 | |
CN111235092B (zh) | 囊胚培养液及其制备方法 | |
Dandekar et al. | Development of two-cell mouse embryos in protein-free and protein-supplemented media | |
RU2778147C1 (ru) | Питательная среда для культивирования ооцитов крупного рогатого скота in vitro | |
CN111172099B (zh) | 配子缓冲液及其制备方法 | |
CN116804182A (zh) | 一种小鼠胚胎用一步式培养液及其制备方法和应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20210326 Address after: 528300 room 413, 4th floor, block 22, Shunlian Machinery City, No.18, Xingye 4th Road, Guanglong Industrial Park, Chihua community, Chencun Town, Shunde District, Foshan City, Guangdong Province Applicant after: Foshan Fukang Biotechnology Co.,Ltd. Address before: 510000 room A219, 29 Guangyun Road, Huangshi street, Baiyun District, Guangzhou City, Guangdong Province Applicant before: Guangzhou Yukang Biotechnology Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231129 Address after: Room 601, No. 6 Yonghe Yushan International Rongjing 1st Road, Xintang Town, Zengcheng District, Guangzhou City, Guangdong Province, 511300, self-made A1 Patentee after: GUANGZHOU FUKANG BIOTECHNOLOGY Co.,Ltd. Address before: 528300 room 413, 4th floor, block 22, Shunlian Machinery City, No.18, Xingye 4th Road, Guanglong Industrial Park, Chihua community, Chencun Town, Shunde District, Foshan City, Guangdong Province Patentee before: Foshan Fukang Biotechnology Co.,Ltd. |
|
TR01 | Transfer of patent right |