CN111067859A

CN111067859A - Polypeptide acne-removing anti-allergy skin care product and preparation method thereof

Info

Publication number: CN111067859A
Application number: CN202010039505.4A
Authority: CN
Inventors: 曾德文; 莫亚琴; 陈秀森
Original assignee: Shanghai Zhongkuang Biotechnology Co Ltd
Current assignee: Shanghai Zhongkuang Biotechnology Co Ltd
Priority date: 2020-01-15
Filing date: 2020-01-15
Publication date: 2020-04-28

Abstract

The invention relates to the technical field of skin care products, and particularly relates to a polypeptide acne-removing and anti-allergy skin care product and a preparation method thereof. The polypeptide acne-removing and anti-allergy skin care product at least comprises the following components in parts by weight: 100 parts of water, 0.01-1 part of active polypeptide, 1-5 parts of fructo-oligosaccharide, 0.1-5 parts of saffron extract, 0.5-5 parts of lactobacillus/mung bean seed extract fermentation product filtrate, 1-5 parts of micromolecular acid, 2-8 parts of humectant and 1-5 parts of preservative. The invention provides a polypeptide acne-removing and allergy-resisting skin care product, which effectively promotes skin healing, improves skin conditions, has the effects of reducing skin redness and swelling, removing acne and resisting allergy, can effectively improve skin darkness, roughness and sensitivity and remove free radicals, and has special fragrance, soft skin feel, good spreadability and easy absorption.

Description

Polypeptide acne-removing anti-allergy skin care product and preparation method thereof

Technical Field

The invention relates to the technical field of skin care products, and particularly relates to a polypeptide acne-removing and anti-allergy skin care product and a preparation method thereof.

Background

With the development of economy and the continuous improvement of life quality, the acne removing consciousness of Chinese acne patients is improved year by year. The combined survey results of the Chinese dermatosis society and the Chinese teenager union show that the incidence of acne in China is on a rising trend year by year, more than 80% of young people suffer from the problem of acne in different degrees, and the serious disease condition of the young people is more than 1/3. Acne is a common chronic skin disease, the number of teenagers is the highest, the acne is frequently found on the face, chest and back, and various skin injuries such as blackheads, papules, pustules, nodules, cysts, scars and the like are shown, so that the acne hinders the appearance. The main cause of the whelk is acne formed by sebum accumulation due to the blockage of pores caused by the accumulation of sebum, and once infection occurs, the whelk is worsened and forms a serious cyst or abscess in a short time.

The market already has more products declared as acne removing, acne resisting and the like, and the products have three forms of medicament, cosmeceutical and cosmetic. Compared with medicines or cosmeceuticals, the cosmetics can be used daily, so that the products are various. The types and the contents of the effective components in the acne-removing cosmetics not only determine the quality of the acne-removing effect, but also have certain influence on the health of consumers, and also have higher safety requirements, so that the acne-removing cosmetics are not only safe to use, but also cannot generate obvious stimulation and damage to the application part and any drug resistance. In order to achieve the curative effect of the medicine, some illegal merchants forbid adding forbidden substances such as antibiotics, glucocorticoids, metronidazole and the like into cosmetic acne-removing products. The substances have obvious temporary acne removal, but can induce dysbacteriosis, easily cause contact dermatitis, antibiotic allergy and other symptoms, and can cause serious internal organ injury and endanger life after long-term use.

Therefore, the development of a skin care product which has soft skin feel, good spreadability and easy absorption, effectively promotes skin healing, improves skin conditions, reduces skin redness and swelling, removes acne and resists allergy is urgently needed.

Disclosure of Invention

In order to solve the technical problems, the invention provides a polypeptide acne-removing and anti-allergy skin care product which at least comprises the following components in parts by weight: 0.01-1 part of active polypeptide, 1-5 parts of fructo-oligosaccharide, 0.1-5 parts of saffron extract, 0.5-5 parts of lactobacillus/mung bean seed extract fermentation product filtrate, 1-5 parts of micromolecular acid, 2-8 parts of humectant, 1-5 parts of preservative and 100 parts of water.

As a preferred technical scheme of the invention, the molecular weight of the active polypeptide is 4-10 KDa.

In a preferred embodiment of the present invention, the active polypeptide is an extracellular matrix polypeptide derived from an animal.

In a preferred embodiment of the present invention, the raw material for preparing the animal-derived extracellular matrix polypeptide is an animal-derived tissue selected from at least one of porcine small intestine submucosa matrix, porcine peritoneal matrix, bladder submucosa matrix, and tissue basement membrane.

In a preferred embodiment of the present invention, the small molecule acid is at least one selected from the group consisting of caprylylsalicylic acid, salicylic acid, mandelic acid, tartaric acid and lactobionic acid.

As a preferable technical scheme of the invention, the humectant is a combination of sodium hyaluronate and glycerin, and the weight ratio is (0.1-1): (2-5).

As a preferred technical scheme of the invention, the sodium hyaluronate is a combination of high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, and the weight ratio is (1-5): (0.1-1): 1.

as a preferable technical scheme of the invention, the molecular weight of the high molecular weight sodium hyaluronate is 1300-1700 KDa; the molecular weight of the medium molecular weight sodium hyaluronate is 250-500 KDa; the molecular weight of the low molecular weight sodium hyaluronate is 6-12 KDa.

As a preferable technical scheme of the invention, the weight ratio of fructo-oligosaccharide, saffron extract and lactic acid bacteria/mung bean seed extract fermentation product filtrate is (1.5-2.5): 1: (0.5-1.5).

The second aspect of the invention provides a preparation method of the polypeptide acne-removing and anti-allergy skin care product, which at least comprises the following steps:

(1) mixing the humectant uniformly, then adding water and antiseptic in sequence, heating to 80-90 deg.C under stirring, and continuing stirring for 20-60 min;

(2) cooling the product obtained in step (1) to 40-50 deg.C, sequentially adding active polypeptide, fructo-oligosaccharide, stigma croci Sativi extract, small molecular acid and lactobacillus/semen Phaseoli Radiati extract fermentation product filtrate, stirring, filtering, and discharging.

Has the advantages that: the invention provides a polypeptide acne-removing and anti-allergy skin care product, which effectively promotes skin healing, improves skin conditions, has the effects of reducing skin redness and swelling, removing acne and anti-allergy, can effectively improve skin darkness, roughness and sensitivity, can remove free radicals and the like, and has special fragrance, soft skin feel, good spreadability and easy absorption.

Detailed Description

The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.

The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.

When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.

The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

Approximating language, as used herein throughout the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes portions that are literally received for modification without substantial change in the basic function to which the invention is related. Accordingly, the use of "about" to modify a numerical value means that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.

In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.

In order to solve the technical problems, the invention provides a polypeptide acne-removing and anti-allergy skin care product in a first aspect, which at least comprises the following components in parts by weight: 0.01-1 part of active polypeptide, 1-5 parts of fructo-oligosaccharide, 0.1-5 parts of saffron extract, 0.5-5 parts of lactobacillus/mung bean seed extract fermentation product filtrate, 1-5 parts of micromolecular acid, 2-8 parts of humectant, 1-5 parts of preservative and 100 parts of water.

Preferably, the polypeptide acne-removing and anti-allergy skin care product comprises the following components in parts by weight: 0.1 part of active polypeptide, 2 parts of fructo-oligosaccharide, 1 part of saffron extract, 1 part of lactobacillus/mung bean seed extract fermentation product filtrate, 2 parts of micromolecular acid, 3.45 parts of humectant, 1.5 parts of preservative and water for supplementing 100 parts.

Water (W)

In the invention, the water is deionized water or distilled water.

Preferably, the water is distilled water.

Active polypeptide

In the invention, the molecular weight of the active polypeptide is 4-10 KDa.

Preferably, the active polypeptide is an extracellular matrix polypeptide of animal origin.

Preferably, the raw material for preparing the animal-derived extracellular matrix polypeptide is an animal-derived tissue.

More preferably, the method for producing an extracellular matrix polypeptide of animal origin comprises at least the following steps:

(1) cleaning, cutting and high-temperature treating animal-derived tissues to obtain tissue samples;

(2) carrying out deep cooling treatment on a tissue sample, then unfreezing, and then carrying out physical crushing to obtain powder;

(3) dissolving the powder in water, stirring and emulsifying to obtain slurry;

(4) heating and dissolving the slurry, carrying out suction filtration, collecting filtrate, and drying to obtain a semi-finished product;

(5) and (4) carrying out irradiation treatment on the semi-finished product to obtain the animal source extracellular matrix polypeptide.

Preferably, in the step (1), the tissue of animal origin is at least one selected from the group consisting of porcine small intestine submucosa matrix, porcine peritoneal matrix, bladder submucosa matrix and tissue basement membrane.

More preferably, in the step (1), the tissue of animal origin is a porcine small intestine submucosa matrix.

Preferably, in the step (1), the temperature of the high-temperature treatment is 100-.

More preferably, in the step (1), the temperature of the high-temperature treatment is 120 ℃ and the time is 30 min.

The inventors believe that the high temperature treatment inactivates bacteria and viruses on the feedstock and removes grease.

Preferably, in the step (2), the condition of the cryogenic treatment is 100-60 ℃ below zero for 20-28 h.

More preferably, in the step (2), the cryogenic treatment condition is below 80 ℃ for 24 hours.

Preferably, in the step (2), warm water with the temperature of 25-35 ℃ is adopted for unfreezing, and the material-liquid ratio is (9-13): 1.

more preferably, in the step (2), warm water at 30 ℃ is adopted for thawing, and the ratio of material to liquid is 10: 1.

the feed-liquid ratio refers to the ratio of the mass of a substance to the volume of liquid water serving as a leaching solution, and the unit is g/L.

Preferably, in the step (2), the thawing operation is circulated 2 to 5 times.

The inventors believe that thawing can loosen tissue fibers and structures.

Preferably, in the step (2), the temperature for physical pulverization is 200-60 ℃ below zero.

More preferably, in the step (2), the temperature for physical pulverization is 196 ℃ below zero.

Preferably, in the step (2), the particle size of the powder is not larger than 200 meshes.

Preferably, in the step (3), the water is distilled water.

Preferably, in the step (3), the ratio of powder to water is (1-5): 100.

more preferably, in the step (3), the ratio of powder to water is 4: 100.

preferably, in the step (4), the specific operations of heating and dissolving are as follows: treating the slurry at 80-120 deg.C for 20-40min, cooling to room temperature, and supplementing 100 parts of water; heating to 80-120 deg.C, and treating for 15-25 min.

More preferably, in the step (4), the specific operations of heating and dissolving are as follows: treating the slurry at 100 deg.C for 30min, cooling to room temperature, and supplementing 100 parts of water; then heated to 100 ℃ for treatment for 20 min.

The inventor thinks that the invention heats the slurry to 100 ℃ for 30min to ensure the slurry to be dissolved, and adds 100 parts of purified water to heat to 100 ℃ after cooling for 20min to further promote the complete dissolution of the slurry.

Preferably, in the step (4), the drying is one selected from freeze drying, spray drying and forced air drying.

More preferably, in the step (4), the drying is freeze-drying.

Preferably, in the step (4), the filtration is performed by membrane filtration.

More preferably, the pore size of the filter membrane is 20-40 μm.

More preferably, the pore size of the filter is 30 μm.

Preferably, in the step (6), the radiation treatment has a radiation dose of 25 to 35 KGy.

More preferably, in the step (6), the radiation treatment is performed at a radiation dose of 30 KGy.

Most preferably, the method for preparing the animal-derived extracellular matrix polypeptide comprises the following steps:

(1) cleaning animal derived tissue, cutting into strips with length of 10cm and width of 2cm, and treating at 120 deg.C for 30min to obtain tissue sample;

(2) the tissue sample is immediately put into subzero treatment at minus 80 ℃ for 24 hours, and then is treated by warm water at 30 ℃ according to the weight ratio of 10: 1, unfreezing the tissue sample according to a proportion, circulating for 3 times in this way, and then physically crushing at-196 ℃ to prepare powder particles with the particle size of below 200 meshes;

(3) mixing the powder particles according to a mass ratio of 4: 100 is mixed in pure water, and is emulsified for 5min at 21000rpm to obtain slurry;

(4) heating the slurry to 100 deg.C, treating for 30min, cooling, adding 100 parts of pure water, heating to 100 deg.C, treating for 20min, filtering with 30 μm filter membrane, collecting filtrate, and freeze drying to obtain powder semi-finished product;

(5) and (3) carrying out 30KGy irradiation treatment on the powder semi-finished product to obtain the animal source extracellular matrix polypeptide.

The invention takes natural animal-derived tissues as polypeptide sources, adopts a set of combined physical preparation technology, comprises high-temperature treatment, repeated ultralow-temperature circulating freezing, physical crushing, high-speed emulsification, freeze drying and ray irradiation methods, reduces the cost and the use of chemical reagents, prepares the ultra-low molecular weight sterile composite polypeptide rich in the type III collagen sequence, and finally achieves the effect of transdermal absorption. The inventor believes that the step (1) of high temperature treatment can inactivate bacteria and viruses on raw materials and remove grease; the circulating cryogenic treatment in the step (2) can loosen tissue fibers and structures and prepare active polypeptide with lower molecular weight. In addition, the selected natural animal source tissue raw materials, especially the porcine small intestine submucosa matrix, comprises the components of type I collagen, type III collagen and type IV collagen, the low molecular weight active polypeptide prepared by the natural animal source tissue raw materials has long metabolic cycle, long activity maintaining time and lower cost, forms functional polypeptide for compatibility use, has various biological functions of resisting bacteria, fungi, viruses and the like, especially has the best removal effect on acne bacillus, particle propionibacterium, acne propionibacterium, staphylococcus aureus and staphylococcus epidermidis, and can also obviously reduce the efficacy and the potential of inflammation caused by the bacteria, fungi, viruses and the like.

Fructo-oligosaccharide

The fructo-oligosaccharide is also called oligofructose or sucrose triose family oligosaccharide, the oligofructose is present in a plurality of fruits and vegetables which are generally eaten, sucrose molecule is kestose formed by combining β - (1 → 2) glycosidic bonds with 1-3 fructose molecules, and the sucrose tetrasaccharide and the sucrose pentasaccharide belong to straight chain heterooligosaccharide consisting of fructose and glucose, and the molecular formula is G-F-Fn (n is 1, 2, 3, G is glucose, and F is fructose).

In the present invention, the source of the fructo-oligosaccharide is not particularly limited, but may be, but not limited to, Quantum Kogaku (China) Bio-Rad.

Saffron extract

The main component of the saffron extract is crocin, which is the main chemical component of saffron with the functions of medicine, nourishing, preserving health, beautifying and nourishing face. The application range of crocin and crocin extract is wide, and the crocin and crocin extract can be used in the aspects of food, health products, medicines, daily chemicals, cosmetics, dyes and the like.

In the present invention, the origin of said saffron extract is not particularly limited, but there may be mentioned, but not limited to, knoishi (tin-free) pharmaceutical company, ltd.

Lactic acid bacteria/mung bean seed extract fermentation product filtrate

In the present invention, the source of the filtrate of the lactic acid bacteria/mung bean seed extract fermentation product is not particularly limited, and there can be mentioned, but not limited to, obsidian biotechnology limited.

In the invention, the weight ratio of the fructo-oligosaccharide, the saffron extract and the filtrate of the lactic acid bacteria/mung bean seed extract fermentation product is (1.5-2.5): 1: (0.5-1.5).

Preferably, the weight ratio of the fructo-oligosaccharide, the saffron extract and the filtrate of the lactic acid bacteria/mung bean seed extract fermentation product is 2: 1: 1.

the inventor conjectures that the compounding of the saffron extract and the filtrate of the lactobacillus/mung bean seed extract fermentation product can maintain the fluidity and the structural integrity of cell membranes, enhance the signal transduction of the cell membranes, promote the absorption of the product by skin, and facilitate the entry of various effective components into cells through the cell membranes to play a role, the saffron extract has the effects of promoting blood circulation to remove blood stasis, accelerating blood circulation, promoting skin metabolism, further accelerating metabolism to transport deposited fat and melanin, has the effects of resisting oxidation, enhancing the integrity of skin cells, and repairing skin barriers, and has remarkable soothing effect on skin, the filtrate fermented by lactobacillus and mung bean has the effects of regulating skin oil composition, promoting the synthesis of collagen, promoting the proliferation of fibroblasts, and enhancing the skin barrier function, so that the oligosaccharide can effectively inhibit the growth of surface bacteria, has the synergistic effect of the effective components of the lactobacillus/mung bean seed extract, can better inhibit the weight of the acne and reduce the recurrence of acne, and the recurrence of the lactobacillus/mung bean seed extract is more easily reduced than the polysaccharide-polysaccharide.

Small molecule acid

In the invention, the small molecule acid is at least one selected from caprylylsalicylic acid, salicylic acid, mandelic acid, fruit acid and lactobionic acid.

Preferably, the small molecule acid is salicylic acid.

The inventor finds that the invention can remove skin cutin, improve whelk and promote skin metabolism by adopting a proper amount of salicylic acid. The inventors believe that the possible reasons are that salicylic acid reduces desmosomal connections between keratinocytes, increases the rate of metabolism of the epithelial cells, accelerates the shedding of aged keratinocytes, promotes skin renewal, and also makes the epithelial cells more regularly arranged, and the stratum corneum thus becomes smooth and fine. In addition, the salicylic acid is fat-soluble, can permeate into the deep layer of pores along sebaceous glands secreting fat, is favorable for dissolving old accumulated cuticle in the pores, improves pore blockage, blocks acne formation and reduces enlarged pores. Meanwhile, the salicylic acid acts on the cells of the hair follicle wall, can help to dredge the blocked hair follicle, repair abnormal cell shedding, prevent the pore blockage caused by acne, is most effective for blackhead acne, can reduce the abnormal shedding phenomenon of the hair follicle wall, prevent the generation of new focuses, but has no effect on reducing sebum secretion and eliminating acne bacillus, only cleans aged cutin, and ensures that the skin is not easy to generate acne.

Moisture-retaining agent

In the invention, the humectant is a combination of sodium hyaluronate and glycerin, and the weight ratio is (0.1-1): (2-5).

Preferably, the humectant is a combination of sodium hyaluronate and glycerin, and the weight ratio is 0.45: 3.

preferably, the sodium hyaluronate is a combination of high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, and the weight ratio is (1-5): (0.1-1): 1.

more preferably, the sodium hyaluronate is a combination of high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, and the weight ratio is 3: 0.5: 1.

preferably, the high molecular weight sodium hyaluronate has a molecular weight of 1300-1700 KDa; the molecular weight of the medium molecular weight sodium hyaluronate is 250-500 KDa; the molecular weight of the low molecular weight sodium hyaluronate is 6-12 KDa.

More preferably, the high molecular weight sodium hyaluronate has a molecular weight of 1500 KDa; the molecular weight of the medium molecular weight sodium hyaluronate is 340 KDa; the molecular weight of the low molecular weight sodium hyaluronate is 10 KDa.

The source of the sodium hyaluronate is not particularly limited, and there may be mentioned, but not limited to, Shandong Furirida pharmaceutical group company.

Preferably, the weight ratio of the humectant to the salicylic acid is (3-4): (1.5-2.5).

More preferably, the weight ratio of the humectant to salicylic acid is 3.45: 2.

the inventor finds that the skin defense force is deteriorated and erythema, pruritus, stabbing pain or allergy phenomena can occur when salicylic acid is used for removing the cuticle, but the invention adopts the compound use of glycerin, high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, particularly the compound use of the high molecular weight sodium hyaluronate with the molecular weight of 1300-. The inventor conjectures that the high molecular weight sodium hyaluronate can prevent the moisture in the skin from evaporating to achieve the purpose of moisturizing by forming a transparent hydrated film on the surface layer of the skin, can block the invasion of external bacteria, dust and ultraviolet rays, and can protect the skin from being damaged; the sodium hyaluronate of the medium molecule can keep the moisture content in the skin relatively stable, and the intercellular and intracellular moisture is maintained at proper level; the low molecular weight sodium alginate has very low molecular weight, can enter the skin through transdermal absorption to deeply moisturize, has the effects of slightly expanding capillary vessels, increasing blood circulation, improving intermediate metabolism, promoting skin nutrition absorption, has a strong wrinkle removing function, can increase skin elasticity and delay skin aging. In the invention, sodium hyaluronate has different molecular weights and different structures and moisturizing mechanisms, thereby showing different moisturizing effects and having remarkable prevention and repair effects on dark pox marks, sunken scars or hypertrophic scars left after healing of the pox, thereby achieving the effects of resisting aging and repairing skin. In addition, the excellent barrier properties of the skin almost completely limit the absorption of these nutrients due to the properties of some biological macromolecules and hydrophilicity, while the amphiphilic nature of hyaluronic acid helps it bind to the cell membrane, reducing the strength of the skin barrier, promoting the skin's osmotic function. The skin moisturizes and transfers protein and hyaluronic acid together, which is helpful for the biomacromolecule to penetrate through the horny layer.

Preservative

In the present invention, the preservative is not particularly limited.

Preferably, the preservative contains a hydroxyl group.

More preferably, the preservative is a combination of pentanediol, hexanediol, and p-hydroxyacetophenone in a weight ratio of 1: (0.5-1.5): (1.8-2).

More preferably, the preservative is pentanediol, hexanediol and p-hydroxyacetophenone in a weight ratio of 1: 1: 1.

the inventor unexpectedly finds that the compounding of the pentanediol and the hexanediol with the p-hydroxyacetophenone not only can keep the stability of the cosmetics, but also can play the roles of resisting oxidation and relieving. The inventors speculate that the possible reason is the combination of pentanediol, hexanediol and p-hydroxyacetophenone, especially when the amount ratio is 1: 1: 1, the growth rate of microorganisms can be influenced, the growth of mould spores is inhibited, the stable property of the skin care product is kept, the skin care product can resist secondary pollution after being used after being uncovered, the skin care product is not easy to deteriorate, and the storage time is prolonged; after the skin care cream is smeared on skin, free radicals in cells can be eliminated, the activity of the cells is maintained, more extracellular matrix and collagen are secreted, so that the structural integrity of the skin is effectively maintained, and the effects of oxidation resistance and relieving are achieved.

Preferably, the preparation method of the polypeptide acne-removing and anti-allergy skin care product at least comprises the following steps:

(1) mixing the humectant uniformly, then adding water and antiseptic in sequence, heating to 85 deg.C while stirring, and continuing stirring for 40 min;

(2) cooling the product obtained in the step (1) to 45 ℃, sequentially adding active polypeptide, fructo-oligosaccharide, saffron extract, micromolecular acid and lactobacillus/mung bean seed extract fermentation product filtrate, uniformly stirring, filtering and discharging to obtain the product.

The inventor finds in the experimental process that the antibacterial activity of the active polypeptide is reduced and about 30% of the antibacterial activity is lost when the active polypeptide is treated at 80-90 ℃, and the antibacterial activity is basically not changed when the active polypeptide is treated at 40-50 ℃; secondly, the invention adopts 1-5% of small molecular acid, so that the pH value of the skin care product is in a near-neutral environment of 6.5-7.5, the antibacterial activity of the active polypeptide is strongest, and the antibacterial activity of the active polypeptide is reduced in a slightly acidic (pH <5.0) or slightly basic (pH >8.0) environment. Under the environment with proper pH value, the antibacterial peptide molecule can better maintain the amphipathy of the functional structure and can adopt the optimal functional conformation, thereby showing the high-efficiency antibacterial action.

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.

In addition, the starting materials used are all commercially available, unless otherwise specified.

Examples

Example 1

Embodiment 1 provides a polypeptide acne-removing and anti-allergy skin care product, which comprises the following components in parts by weight: 0.1 part of active polypeptide, 2 parts of fructo-oligosaccharide, 1 part of saffron extract, 1 part of lactobacillus/mung bean seed extract fermentation product filtrate, 2 parts of micromolecular acid, 3.45 parts of humectant, 1.5 parts of preservative and water for supplementing 100 parts.

The water is distilled water.

The preparation method of the active polypeptide comprises the following steps:

(1) cleaning porcine small intestine submucosa matrix, cutting into strips with length of 10cm and width of 2cm, and treating at 120 deg.C for 30min to obtain tissue sample;

(4) heating the serous fluid to 100 deg.C, treating for 30min, cooling, adding 100 parts of pure water, heating to 100 deg.C, treating for 20min, filtering with 30 μm nylon gauze, collecting filtrate, and freeze drying to obtain powder semi-finished product;

The fructooligosaccharides are purchased from Quangagagaku (China) Bio-products Ltd.

Said saffron extract was purchased from knoishi (tin-free) pharmaceutical limited.

The lactobacillus/mung bean seed extract fermentation product filtrate was purchased from obsidian biotechnology limited.

The weight ratio of the fructo-oligosaccharide, the saffron extract and the filtrate of the lactic acid bacteria/mung bean seed extract fermentation product is 2: 1: 1.

the micromolecular acid is salicylic acid.

The humectant is a combination of sodium hyaluronate and glycerin, and the weight ratio is 0.45: 3; the sodium hyaluronate is a combination of high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, and the weight ratio is 3: 0.5: 1; the molecular weight of the high molecular weight sodium hyaluronate is 1500 KDa; the molecular weight of the medium molecular weight sodium hyaluronate is 340 KDa; the molecular weight of the low molecular weight sodium hyaluronate is 10 KDa; the sodium hyaluronate was purchased from Shandong Furuida pharmaceutical group.

The weight ratio of the humectant to the salicylic acid is 3.45: 2.

the preservative is a combination of pentanediol, hexanediol and p-hydroxyacetophenone, and the weight ratio is 1: 1: 1.

the preparation method of the polypeptide acne-removing and anti-allergy skin care product comprises the following steps:

Example 2

Embodiment 2 provides a polypeptide acne-removing and anti-allergy skin care product, which comprises the following components in parts by weight: 0.01 part of active polypeptide, 1 part of fructo-oligosaccharide, 0.1 part of saffron extract, 0.5 part of lactic acid bacteria/mung bean seed extract fermentation product filtrate, 1 part of micromolecular acid, 2 parts of humectant, 1 part of preservative and the balance of water to 100 parts.

The water is distilled water.

The preparation method of the active polypeptide comprises the following steps:

The micromolecular acid is salicylic acid.

The humectant is a combination of sodium hyaluronate and glycerin, and the weight ratio is 0.1: 2; the sodium hyaluronate is a combination of high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, and the weight ratio is 1: 0.1: 1; the molecular weight of the high molecular weight sodium hyaluronate is 1500 KDa; the molecular weight of the medium molecular weight sodium hyaluronate is 340 KDa; the molecular weight of the low molecular weight sodium hyaluronate is 10 KDa; the sodium hyaluronate was purchased from Shandong Furuida pharmaceutical group.

The weight ratio of the humectant to the salicylic acid is 3: 1.5.

the preservative is a combination of pentanediol, hexanediol and p-hydroxyacetophenone, and the weight ratio is 1: 0.5: 1.8.

Example 3

Embodiment 3 provides a polypeptide acne-removing and anti-allergy skin care product, which comprises the following components in parts by weight: 1 part of active polypeptide, 5 parts of fructo-oligosaccharide, 5 parts of saffron extract, 5 parts of lactobacillus/mung bean seed extract fermentation product filtrate, 5 parts of micromolecular acid, 8 parts of humectant, 5 parts of preservative and the balance of water to 100 parts.

The water is distilled water.

The preparation method of the active polypeptide comprises the following steps:

The weight ratio of the fructo-oligosaccharide, the saffron extract and the filtrate of the lactic acid bacteria/mung bean seed extract fermentation product is 2.5: 1: 1.5.

the micromolecular acid is salicylic acid.

The humectant is a combination of sodium hyaluronate and glycerin, and the weight ratio is 1: 5; the sodium hyaluronate is a combination of high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, and the weight ratio is 5: 1: 1; the molecular weight of the high molecular weight sodium hyaluronate is 1500 KDa; the molecular weight of the medium molecular weight sodium hyaluronate is 340 KDa; the molecular weight of the low molecular weight sodium hyaluronate is 10 KDa; the sodium hyaluronate was purchased from Shandong Furuida pharmaceutical group.

The weight ratio of the humectant to the salicylic acid is 4: 2.5.

the preservative is a combination of pentanediol, hexanediol and p-hydroxyacetophenone, and the weight ratio is 1: 1.5: 2.

Example 4

Example 4 differs from example 1 in that the polypeptide acne-removing and anti-allergy skin care product at least comprises the following components in parts by weight: 0.1 part of active polypeptide, 1.5 parts of fructo-oligosaccharide, 1 part of saffron extract, 0.5 part of lactic acid bacteria/mung bean seed extract fermentation product filtrate, 1.5 parts of micromolecular acid, 3 parts of humectant, 1.5 parts of preservative and the balance of water to 100 parts.

Example 5

Example 5 differs from example 1 in that the polypeptide acne-removing and anti-allergy skin care product at least comprises the following components in parts by weight: 0.1 part of active polypeptide, 2.5 parts of fructo-oligosaccharide, 1 part of saffron extract, 1.5 parts of lactobacillus/mung bean seed extract fermentation product filtrate, 2.5 parts of micromolecular acid, 4 parts of humectant, 1.5 parts of preservative and the balance of water to 100 parts.

Example 6

Example 6 differs from example 1 in that it does not contain an active polypeptide.

Example 7

Example 7 differs from example 1 in that it does not contain fructooligosaccharides.

Example 8

Example 8 differs from example 1 in that it does not contain saffron extract.

Example 9

Example 9 differs from example 1 in that the lactic acid bacteria/mung bean seed extract fermentation product filtrate is not contained.

Example 10

Example 10 differs from example 1 in that no small molecule acid is included.

Example 11

Example 11 differs from example 1 in that sodium hyaluronate is not present.

Example 12

Example 12 differs from example 1 in that the sodium hyaluronate has a molecular weight of 1500 KDa.

Example 13

Example 13 differs from example 1 in that the sodium hyaluronate has a molecular weight of 340 KDa.

Example 14

Example 14 differs from example 1 in that the sodium hyaluronate has a molecular weight of 10 KDa.

Example 15

Example 15 differs from example 1 in that the preservative is p-hydroxyacetophenone.

Example 16

Example 16 differs from example 1 in that the weight part of the small molecule acid is increased to 15 parts.

Example 17

Example 17 differs from example 1 in that the preparation method of the polypeptide acne-removing and anti-allergy skin care product comprises the following steps: mixing humectant uniformly, adding water and antiseptic in sequence, heating to 85 deg.C under stirring, adding active polypeptide, fructo-oligosaccharide, stigma croci Sativi extract, small molecular acid and lactobacillus/semen Phaseoli Radiati extract fermentation product filtrate, stirring for 40min, cooling to room temperature, filtering, and discharging.

Performance testing

1. Product safety evaluation (human body skin patch experiment)

Firstly, testing a test article: skin care products prepared in examples 1-5.

The test population: 100 persons, half each of male and female; age: between 25 and 35 years of age.

Health condition: the skin of the subject is healthy, has no allergic history of the skin disease, and meets the voluntary selection standard of the subject.

Grouping: 5 groups of 20 persons each, mean age 30 ± 5 years.

Secondly, a spot pasting method: selecting a qualified spot tester, dripping 0.03g of a test object into the spot tester in a closed spot-patch test mode, covering the back of the test object with an externally-applied special adhesive tape, removing the test object after 24 hours, respectively recording the skin reactions of the test object after 0.5, 6, 12, 24 and 48 hours after removal, and recording the results according to the skin reaction grading standard in the cosmetic hygiene standard.

Thirdly, experimental results: the result of the human body skin patch test shows that: the skin reactions of all the groups of subjects are observed at 0.5 hour, 6 hours, 12 hours, 24 hours and 48 hours through the patch tests of the compositions prepared in the examples 1 to 5 respectively, wherein 0 case has adverse skin reactions, which indicates that the acne-removing and anti-allergy skin care product is safe to use.

2. Evaluation of Permeability, absorbability and anti-acne Effect

Firstly, an experimental article: the skin care products prepared in examples 1 and 6-17.

II, the tested people group: 130 persons, half each of male and female; age: between 25 and 35 years of age.

The selected volunteers had whelks in their faces and had not used any product for treating whelks for the previous month of the test.

Grouping: 13 groups of 10 people each, mean age 30 ± 5 years.

Thirdly, experimental samples and experimental methods:

each group was applied to the face once a day, morning and evening, using the skin care products prepared in examples 1 and 6-17, respectively.

Fourthly, experiment time: and 14 days.

Fifthly, the standard of experimental results:

absorbency, permeability: good, better, general, poor;

the acne removing effect is as follows: valid and invalid.

Sixthly, experimental results:

the results of the absorption, permeability and anti-acne effect tests of the skin care products provided by the examples of the invention on the volunteers are shown in table 1.

The evaluation of the effect using the volunteer of example 1 is shown in Table 2.

TABLE 1 results of the Performance test of skin care products obtained in examples 1, 6 to 17 (numerical units: human)

	Good/human	Better/human	General/human	Poor/human	Effective in removing acne	Acne eliminating effect/human
							Example 1	10	0	0	0	10	0
Example 6	8	2	0	0	1	9
							Example 7	8	1	1	0	8	2
Example 8	8	1	1	0	7	3
							Example 9	9	1	0	0	8	2
Example 10	7	1	2	0	6	4
							Example 11	8	1	1	0	6	4
Example 12	6	2	1	1	8	2
							Example 13	7	1	2	0	7	3
Example 14	9	1	0	0	8	2
							Example 15	/	/	/	/	9	1
Example 16	/	/	/	/	8	2
							Example 17	/	/	/	/	6	4

Table 2. evaluation of acne removing Effect of skin care products according to example 1

The acne-removing skin care product disclosed by the invention can well penetrate through the surface layer of the skin and enter the deep layer to be absorbed; the components are matched according to a specific proportion, and the rich multiple functional components are mutually blended and cooperated, so that the effects of peeling aged cutin, dredging pores, inhibiting bacteria, diminishing inflammation, controlling oil, promoting metabolism, removing pigmentation caused by acne growth and the like in all directions and multiple angles can be achieved, and the acne removing, anti-allergy, repairing and skin caring effects are excellent.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims

1. The polypeptide acne-removing and anti-allergy skin care product is characterized by at least comprising the following components in parts by weight: 0.01-1 part of active polypeptide, 1-5 parts of fructo-oligosaccharide, 0.1-5 parts of saffron extract, 0.5-5 parts of lactobacillus/mung bean seed extract fermentation product filtrate, 1-5 parts of micromolecular acid, 2-8 parts of humectant, 1-5 parts of preservative and 100 parts of water.

2. The polypeptide acne-removing and anti-allergy skin care product according to claim 1, wherein the molecular weight of the active polypeptide is 4-10 KDa.

3. The polypeptide acne-removing and anti-allergy skin care product according to claim 1, wherein the active polypeptide is an animal-derived extracellular matrix polypeptide.

4. The polypeptide skin care product for removing acne and resisting allergy according to claim 3, wherein the raw material for preparing the animal-derived extracellular matrix polypeptide is animal-derived tissue selected from at least one of porcine small intestine submucosa matrix, porcine peritoneal matrix, bladder submucosa matrix and tissue basement membrane.

5. The polypeptide acne-removing and anti-allergy skin care product according to claim 1, wherein the small molecular acid is at least one selected from caprylylsalicylic acid, salicylic acid, mandelic acid, fruit acid and lactobionic acid.

6. The polypeptide acne-removing and allergy-resisting skin care product according to claim 1, wherein the moisturizing agent is a combination of sodium hyaluronate and glycerin, and the weight ratio is (0.1-1): (2-5).

7. The polypeptide acne-removing and allergy-resisting skin care product according to claim 6, wherein the sodium hyaluronate is a combination of high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, and the weight ratio of the sodium hyaluronate is (1-5): (0.1-1): 1.

8. the polypeptide acne-removing and anti-allergy skin care product according to claim 1, wherein the molecular weight of the high molecular weight sodium hyaluronate is 1300-1700 KDa; the molecular weight of the medium molecular weight sodium hyaluronate is 250-500 KDa; the molecular weight of the low molecular weight sodium hyaluronate is 6-12 KDa.

9. The polypeptide acne-removing and anti-allergy skin care product according to claim 1, wherein the weight ratio of the fructo-oligosaccharide to the filtrate of the saffron extract and the filtrate of the lactic acid bacteria/mung bean seed extract fermentation product is (1.5-2.5): 1: (0.5-1.5).

10. A method for preparing the polypeptide acne-removing and anti-allergy skin care product according to any one of claims 1 to 9, which is characterized by at least comprising the following steps: