CN111135118A

CN111135118A - Anti-aging polypeptide composition

Info

Publication number: CN111135118A
Application number: CN202010060231.7A
Authority: CN
Inventors: 曾德文; 莫亚琴
Original assignee: Shanghai Zhongkuang Biotechnology Co Ltd
Current assignee: Shanghai Zhongkuang Biotechnology Co Ltd
Priority date: 2020-01-19
Filing date: 2020-01-19
Publication date: 2020-05-12

Abstract

The invention relates to the technical field of skin care products, and particularly relates to an anti-aging polypeptide composition. Comprises the following components in parts by weight: 0.2-0.7 part of humectant A, 0.03-0.35 part of active polypeptide and 0.5-2 parts of swertiamarin. The composition obtained by the invention can enable the skin to have the real functions of locking water and preserving moisture, keep the skin moist and elastic, and simultaneously stimulate skin cells to synthesize more type III collagen, thereby preventing the skin from aging.

Description

Anti-aging polypeptide composition

Technical Field

The invention relates to the technical field of skin care products, and particularly relates to an anti-aging polypeptide composition.

Background

Aging is not a disease but a physiological phenomenon. The skin is located on the body surface and is one of the most prominent organs in the aging process of the body. The skin is directly contacted with the external environment and is stimulated by various tension factors such as oxidation, dryness, ultraviolet rays and the like, so that the skin is very easily damaged. Meanwhile, the skin is easy to age due to the decline of skin function and genetic teratogenesis caused by the influence of internal factors such as age, diet and living habits. The main characteristics of human aging are: thinning skin, wrinkles, decreased skin elasticity, pigmentation, muscle atrophy, decreased skin water retention, etc. At present, people live more stably and abundantly, the requirement for beautifying the appearance is higher and higher, and people want to be youthful forever, so that various methods for delaying aging, such as using anti-aging drugs and anti-aging cosmetics, are searched for in all directions. The anti-aging effect of the commercially available anti-aging cosmetics is general, or various chemically synthesized hormones are contained therein, which easily causes damage to the body and is difficult to satisfy the needs of users.

Disclosure of Invention

In order to solve the above technical problems, the first aspect of the present invention provides an anti-aging polypeptide composition, comprising at least: 0.2-0.7 part of humectant A, 0.03-0.35 part of active polypeptide and 0.5-2 parts of swertiamarin.

As a preferred technical solution of the present invention, the moisturizer a is selected from one or more of sodium hyaluronate, oat glucan, ceramide and isopropyl myristate.

As a preferred technical scheme of the present invention, the sodium hyaluronate is a combination of sodium hyaluronate macromolecules, sodium hyaluronate middle molecules, and sodium hyaluronate small molecules.

In a preferred embodiment of the present invention, the active polypeptide is an extracellular matrix polypeptide derived from an animal.

As a preferable technical scheme of the invention, the preparation method of the animal source extracellular matrix polypeptide comprises the following steps:

(1) cleaning, cutting and high-temperature treating animal-derived tissues to obtain tissue samples;

(2) carrying out cryogenic treatment on the tissue sample, unfreezing the tissue sample by using warm water at the temperature of 25-35 ℃, and circulating for 3-5 times to obtain the unfrozen tissue sample;

(3) physically crushing the thawed tissue sample to obtain powder particles;

(4) dissolving the powder particles in water, and stirring and emulsifying at a high speed to obtain slurry;

(5) heating and dissolving the slurry, performing suction filtration, collecting filtrate, and drying to obtain a powder semi-finished product;

(6) and (3) carrying out irradiation treatment on the powder semi-finished product to obtain the animal source extracellular matrix polypeptide.

In a second aspect, the invention provides an anti-aging essence, which is prepared from the raw materials including the polypeptide composition capable of resisting aging.

As a preferable technical scheme, the anti-aging essence comprises, by weight, 0.5-5 parts of a humectant B, 0.1-1 part of an antioxidant, 0.5-4 parts of an anti-aging polypeptide composition, and water in an amount up to 100 parts.

In a preferred embodiment of the present invention, the humectant B is one or more selected from glycerin, hexylene glycol, pentylene glycol, dipropylene glycol, butylene glycol, rose water, and propylene glycol.

As a preferable technical scheme of the invention, the antioxidant is selected from one or more of tocopherol acetate, vitamin C, vitamin E and p-hydroxyacetophenone.

The third aspect of the invention provides a preparation method of anti-aging essence, which comprises the following steps:

(1) mixing the humectant A and glycerol uniformly in advance;

(2) sequentially adding water, hexanediol, pentanediol, an antioxidant and the mixture into an emulsifying pot, starting stirring, and controlling the temperature to be 70-90 ℃;

(3) after the raw materials of the emulsifying pot are completely dissolved, starting to cool, adding active polypeptide and swertiamarin when the temperature is reduced to 40-50 ℃, and uniformly stirring;

(4) when the temperature is reduced to 35-45 ℃, sampling and detecting, and filtering and discharging after the temperature is qualified to obtain the product.

Has the advantages that: the invention provides an anti-aging polypeptide composition, which is prepared by firstly adopting a special preparation method to obtain active polypeptide containing a large number of small molecules, and by adding sodium hyaluronate and swertiamarin with different molecular weights and reasonably regulating and controlling the mass ratio of the sodium hyaluronate and the swertiamarin, the anti-wrinkle moisturizing polypeptide composition with good effects is obtained. The invention further provides the anti-aging essence containing the anti-aging composition, tests show that the anti-wrinkle moisturizing and anti-aging effects are good, and the obtained essence is still homogeneous and stable after being placed for a long time.

Detailed Description

The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.

The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.

When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.

The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

Approximating language, as used herein throughout the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes portions that are literally received for modification without substantial change in the basic function to which the invention is related. Accordingly, the use of "about" to modify a numerical value means that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.

In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.

In a more preferred embodiment, the anti-aging composition at least comprises 0.3 to 0.5 part of humectant A, 0.05 to 0.2 part of active polypeptide and 0.8 to 1.5 parts of swertiamarin in parts by weight.

In a most preferred embodiment, the anti-aging composition at least comprises 0.45 part of humectant A, 0.1 part of active polypeptide and 1 part of swertiamarin in parts by weight.

Humectant A

The moisturizer is a cosmetic raw material with the functions of moisturizing and repairing sebum membranes, and the raw material has a special molecular structure, can adsorb and retain moisture, and maintains the skin barrier function while maintaining the skin hydration.

In a preferred embodiment, the moisturizer a of the present invention is selected from one or more of sodium hyaluronate, oat glucan, ceramide and isopropyl myristate.

In a more preferred embodiment, the humectant a of the present invention is sodium hyaluronate.

In a most preferred embodiment, the sodium hyaluronate of the present invention is a combination of sodium hyaluronate macromolecules, sodium hyaluronate mesomolecules and sodium hyaluronate micromolecules.

In a preferred embodiment, the sodium hyaluronate is a sodium hyaluronate macromolecule, a sodium hyaluronate middle molecule and a sodium hyaluronate small molecule, wherein the mass ratio of the sodium hyaluronate macromolecule to the sodium hyaluronate middle molecule to the sodium hyaluronate small molecule is (1-5): (0.1-1): 1.

in a more preferred embodiment, the sodium hyaluronate is a combination of sodium hyaluronate macromolecules, sodium hyaluronate middle molecules and sodium hyaluronate small molecules, wherein the mass ratio of the sodium hyaluronate macromolecules to the sodium hyaluronate middle molecules to the sodium hyaluronate small molecules is (2-4): (0.3-0.7): 1.

in a most preferred embodiment, the sodium hyaluronate is a combination of sodium hyaluronate macromolecules, sodium hyaluronate middle molecules and sodium hyaluronate small molecules, wherein the mass ratio of the sodium hyaluronate macromolecules to the sodium hyaluronate middle molecules to the sodium hyaluronate small molecules is 3: 0.5: 1.

in a preferred embodiment, the molecular weight of the sodium hyaluronate macromolecules of the invention is 1200-1700 KDa.

In a more preferred embodiment, the molecular weight of the sodium hyaluronate macromolecules of the invention is 1300-1600 kDa.

In a most preferred embodiment, the sodium hyaluronate macromolecules of the invention have a molecular weight of 1500 KDa.

In a preferred embodiment, the molecular weight of the molecule in the sodium hyaluronate of the present invention is 300-450 KDa.

In a more preferred embodiment, the molecular weight of the molecule in the sodium hyaluronate of the present invention is 300-400 KDa.

In a most preferred embodiment, the molecular weight of the molecule in the sodium hyaluronate of the present invention is 340 kDa.

In a preferred embodiment, the sodium hyaluronate small molecule of the present invention has a molecular weight of 5 to 20 kDa.

In a more preferred embodiment, the sodium hyaluronate small molecule of the present invention has a molecular weight of 8-15 KDa.

In a most preferred embodiment, the sodium hyaluronate small molecule of the present invention has a molecular weight of 10 KDa.

The sodium hyaluronate is an inherent component in a human body, is a glucuronic acid, the moisture level of the skin is closely related to the content of the hyaluronic acid, and the content of the hyaluronic acid in the skin is gradually reduced along with the aging, the working pressure is increased, and the influence of ultraviolet light or the radiation of electronic products is large, so that the water retention function of the skin is weakened, a large number of wrinkles are generated, and the aging of the skin is accelerated. Therefore, in order to prevent skin aging, a skin care product containing sodium hyaluronate is needed, but the effect is not ideal, the moisturizing effect is poor when the sodium hyaluronate with low concentration is added, the phenomenon of suck-back is generated when the sodium hyaluronate with high concentration is used, the moisture in the dermis layer is easily sucked out, and the result is in a negative way; the inventor finds through long-term experiments that the technical problems can be solved and the moisturizing and anti-wrinkle effects can be effectively realized by combining sodium hyaluronate with different molecular weights, and the possible reason is that the small-molecular sodium hyaluronate has strong migration capacity, can quickly permeate into a dermis layer to promote microcirculation of blood in the skin and is beneficial to the skin to fully absorb nutrients; the sodium hyaluronate with medium molecular weight can combine water and has the function of keeping water in or among keratinocytes, repair intercellular substance, promote proliferation and differentiation of epidermal cells and realize skin regeneration; the sodium hyaluronate with large molecular weight has stronger viscoelasticity and lubricity due to the huge molecular structure, is easy to form a layer of breathable film on the surface layer of the skin, can prevent the interference of bacteria, dust and the like to the outside, and can keep the activity of small-molecule sodium hyaluronate and medium-molecule sodium hyaluronate in the inner layer of the skin to the inside; in a word, the synergistic action among hyaluronic acids with different molecular weights can enable the skin to have the functions of really locking and moisturizing, and keep the moisturizing elasticity of the skin, thereby preventing the skin from aging.

Active polypeptide

The active polypeptide is an animal source extracellular matrix polypeptide.

In a preferred embodiment, the method for preparing the extracellular matrix polypeptide of animal origin comprises the steps of:

(3) physically crushing the thawed tissue sample to obtain powder particles;

In a more preferred embodiment, the method for producing an extracellular matrix polypeptide of animal origin according to the present invention comprises the steps of:

(1) cleaning animal-derived tissue, dividing into strips with length of 10cm and width of 2cm, and treating at 110-140 deg.C for 20-40min to obtain tissue sample;

(2) the tissue sample is immediately put into subzero treatment for 24 hours at the temperature of between 90 ℃ below zero and 70 ℃ below zero, and then is treated by warm water at the temperature of 30 ℃ according to the weight ratio of 10: 1, thawing the tissue sample according to the proportion, and circulating for 3-5 times to obtain a thawed tissue sample;

(3) physically crushing the thawed tissue sample at-196 ℃ to prepare powder particles with the particle size of less than 200 meshes;

(4) mixing the powder particles according to a mass ratio of (1: 4): 100 is mixed in pure water, and is emulsified for 5min at 18000-;

(5) heating the slurry to 100 deg.C, treating for 30min, cooling, adding 100 parts of pure water, heating to 80-120 deg.C, treating for 20-50min, vacuum filtering with 20-40 μm filter membrane, collecting filtrate, and freeze drying to obtain powder semi-finished product;

(6) and (3) carrying out 30KGy irradiation treatment on the powder semi-finished product to obtain the animal source extracellular matrix polypeptide.

In a most preferred embodiment, the method for producing an extracellular matrix polypeptide of animal origin according to the present invention comprises the steps of:

(1) cleaning animal derived tissue, cutting into strips with length of 10cm and width of 2cm, and treating at 100 deg.C for 25min to obtain tissue sample;

(2) the tissue sample is immediately put into subzero treatment at-85 ℃ for 24h, and then is treated by warm water at 30 ℃ according to the weight ratio of 10: 1, thawing the tissue sample according to the proportion, and circulating for 4 times in such a way to obtain a thawed tissue sample;

(4) mixing the powder particles according to a mass ratio of 2.5: 100 is mixed in pure water, and is emulsified for 5min at 21000rpm to obtain slurry;

(5) heating the slurry to 100 deg.C, treating for 30min, cooling, adding 100 parts of pure water, heating to 95 deg.C, treating for 30min, vacuum filtering with 30 μm filter membrane, collecting filtrate, and freeze drying to obtain powder semi-finished product;

The inventor finds out through experiments that the treatment of the animal-derived matrix at the temperature of 100-140 ℃ can eliminate bacteria and viruses on the surface of the animal-derived matrix on the one hand, and can realize effective degreasing and improve the sterilization efficiency on the other hand. If the temperature is lower than 100 ℃, the bacillus killing rate is lower, and if the temperature is higher than 140 ℃, the protein structure can be damaged. Then, by the treatments in the steps (2) and (3), powder particles having a uniform particle diameter can be obtained. The possible reason is that when the tissue sample is placed at-90 to-70 ℃ for treatment for 20-28h, then the tissue sample is unfrozen by water at 25-35 ℃, and tissue fibers and structures are loosened after repeating for 2-3 times, so that the tissue sample is deeply crushed; then the mixture is immediately placed at the temperature of-196 ℃ for crushing, and can be crushed into particles with certain particle size, so that the polypeptide can be hydrolyzed into micromolecular polypeptide to the maximum extent, and the biological activity of the micromolecular polypeptide is not influenced.

When the active polypeptide is applied to skin, the invention can effectively regulate and control the skin aging process, prevent skin damage and delay skin aging. The reason for possible existence is that the active polypeptide prepared by the invention has smaller particle size, smaller molecular weight and good transdermal absorption capacity, can promote cell metabolism, improve the living environment of skin, enhance blood circulation and keep the integrity of stratum corneum moisture and fiber structure; the special structure of the skin moisturizing cream has certain water locking capacity, and has synergistic effect with the sodium hyaluronate, so that the skin can be kept in a moist state for a long time, cells are stimulated to synthesize more collagen, the aged skin is filled and repaired, the tension of the skin is enhanced, and the skin moistening elasticity is recovered; when the mass ratio of the sodium hyaluronate to the active polypeptide is (0.2-0.7): (0.03-0.35), the effect is best.

Swertiamarin

The swertiamarin is a cycloolefine ether terpenoid compound separated from swertia pseudochinensis (Swertia patent) which is a Gentianaceae plant. White fine powder, bitter, slightly hygroscopic in air. Is easily soluble in methanol and ethanol, slightly soluble in water, and insoluble in chloroform and petroleum ether. The Chinese medicine composition is clinically applicable to pains caused by gastrointestinal spasm, gastroenteritis, intestinal ascariasis, biliary ascariasis, cholecystitis, cholelithiasis and other biliary tract diseases.

In a preferred embodiment, the swertiamarin of the present invention is commercially available from a source including, but not limited to, International flavor and fragrance company, USA under the designation SWT-7.

The inventor finds in experiments that when the swertiamarin is added into the polypeptide composition and acts on the skin, on one hand, the swertiamarin has the effects of resisting bacteria and diminishing inflammation and can repair skin injury; on the other hand, the invention can also reduce the generation of wrinkles and realize the effect of high-efficiency anti-wrinkle, and the probable reasons are that the iridoid compound is adopted to be matched with the bioactive polypeptide, thereby being beneficial to decomposing and generating the hematin, expanding blood capillaries, activating a cell enzyme system in the skin, increasing the secretion of collagen and elastic fiber protein, effectively preventing the oxidation of protein, protecting the activity of intercellular substance, directly acting on the life cycle of cells, enhancing the supporting effect of the collagen and the elastic fiber protein on the epidermis, improving the outline, increasing the elasticity and the tightness of the skin, and fundamentally solving and fading wrinkles.

The second aspect of the invention provides anti-aging essence which comprises, by weight, 0.5-5 parts of a humectant B, 0.1-1 part of an antioxidant, 0.5-4 parts of an anti-aging polypeptide composition, and water in an amount up to 100 parts.

In a preferred embodiment, the anti-aging essence comprises 3.5 to 4.5 parts by weight of humectant B, 0.3 to 0.8 part by weight of antioxidant, 1 to 3 parts by weight of anti-aging polypeptide composition and water in a balance of 100 parts by weight.

In a most preferred embodiment, the anti-aging essence comprises, by weight, 4 parts of a moisturizing agent B, 0.5 part of an antioxidant, 1.55 parts of an anti-aging polypeptide combination, and water in a balance of 100 parts.

Humectant B

In a preferred embodiment, the humectant B of the present invention is selected from one or more of glycerin, hexylene glycol, pentylene glycol, dipropylene glycol, butylene glycol, rose water, propylene glycol.

In a more preferred embodiment, the humectant B of the present invention is glycerol, hexanediol and pentanediol, wherein the mass ratio of the glycerol to the hexanediol to the pentanediol is (3-8): 1: 1.

in a most preferred embodiment, the humectant B of the present invention is glycerin, hexylene glycol, and pentylene glycol, wherein the mass ratio of glycerin, hexylene glycol, and pentylene glycol is 6: 1: 1.

antioxidant agent

The antioxidant (Antioxidants) of the present invention is a substance that prevents the adverse effects of oxygen. It is a class of substances that helps to trap and neutralize free radicals, thereby removing the damage that free radicals cause to the human body.

In a preferred embodiment, the antioxidant of the present invention is selected from one or more of tocopherol acetate, vitamin C, vitamin E, p-hydroxyacetophenone.

In a most preferred embodiment, the antioxidant of the present invention is p-hydroxyacetophenone.

(1) mixing the humectant A and glycerol uniformly in advance;

In a preferred embodiment, the preparation method of the anti-aging essence comprises the following steps:

(1) mixing the humectant A and glycerol uniformly in advance;

(2) sequentially adding water, hexanediol, pentanediol, an antioxidant and the mixture into an emulsifying pot, starting stirring, and controlling the temperature to be 85 ℃;

(3) after the raw materials of the emulsifying pot are completely dissolved, starting to cool, adding active polypeptide and swertiamarin when the temperature is reduced to 45 ℃, and uniformly stirring;

(4) when the temperature is reduced to 40 ℃, sampling and detecting, and filtering and discharging after the temperature is qualified to obtain the product.

The inventor finds that by adjusting the adding sequence of the components in the anti-aging essence and controlling the temperature, the homogenized and stable essence can be obtained, and the reason for the existence of the components is probably that sodium hyaluronate with different molecular weights is dispersed in glycerin, and is added with water, hexanediol, pentanediol and antioxidant in sequence and stirred at 70-90 ℃ for homogenization; secondly, the active polypeptide and the swertiamarin are simultaneously added and homogenized for the second time at the temperature of 40-50 ℃, the mass ratio of sodium hyaluronate to the swertiamarin needs to be controlled, the dispersibility of the swertiamarin cannot be improved when the sodium hyaluronate is too little, the skin absorption is not facilitated when the sodium hyaluronate is too much, and researches show that when the mass ratio of the sodium hyaluronate to the swertiamarin is (0.2-0.7): (0.5-2), on one hand, the biological activity of the active polypeptide and the swertiamarin is ensured, and on the other hand, the dispersibility and compatibility of the swertiamarin in the essence are improved, so that the homogeneous and stable essence is obtained.

It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.

In addition, the raw materials used are commercially available from national chemical reagents, unless otherwise specified.

Examples

In order to better understand the above technical solutions, the following detailed descriptions will be provided with reference to specific embodiments. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention. In addition, the raw materials are commercially available and the extraction methods of the extract are all conventional extraction methods, if not otherwise specified.

Sodium hyaluronate the following examples are purchased from Shandong Furuida Biotech, Inc.

Example 1

Example 1 provides an anti-aging polypeptide composition, which includes 0.45 part of humectant A, 0.1 part of active polypeptide, and 1 part of swertiamarin.

The humectant A is sodium hyaluronate.

The sodium hyaluronate is a combination of sodium hyaluronate macromolecules, sodium hyaluronate middle molecules and sodium hyaluronate small molecules, wherein the mass ratio of the sodium hyaluronate macromolecules to the sodium hyaluronate middle molecules to the sodium hyaluronate small molecules is 3: 0.5: 1.

the molecular weight of the sodium hyaluronate macromolecules is 1500 KDa.

The molecular weight of the molecule in the sodium hyaluronate is 340 KDa.

The molecular weight of the sodium hyaluronate micromolecules is 10 KDa.

The active polypeptide is an animal source extracellular matrix polypeptide.

The preparation method of the animal source extracellular matrix polypeptide comprises the following steps:

(4) mixing the powder particles according to a mass ratio of 2.5: 100 is mixed in pure water, and is emulsified for 5min at high speed of 21000rpm to obtain slurry;

The swertiamarin can be obtained commercially, and is produced by American International flavor and fragrance company under the brand name SWT-7.

An anti-aging essence comprises 4 parts of a humectant B, 0.5 part of an antioxidant, 1.55 parts of an anti-aging polypeptide composition and water, wherein the total amount of the water is 100 parts.

The humectant B is glycerol, hexanediol and pentanediol, wherein the mass ratio of the glycerol to the hexanediol to the pentanediol is 6: 1: 1.

the antioxidant is p-hydroxyacetophenone.

The preparation method of the anti-aging essence comprises the following steps:

(1) mixing the humectant A and glycerol uniformly in advance;

Example 2

Embodiment 2 provides an anti-aging polypeptide composition, which comprises 0.2 part of humectant A, 0.03 part of active polypeptide and 0.5 part of swertiamarin.

The humectant A is sodium hyaluronate.

the molecular weight of the sodium hyaluronate macromolecules is 1500 KDa.

The molecular weight of the molecule in the sodium hyaluronate is 340 KDa.

The molecular weight of the sodium hyaluronate micromolecules is 10 KDa.

The active polypeptide is an animal source extracellular matrix polypeptide.

the antioxidant is p-hydroxyacetophenone.

The preparation method of the anti-aging essence comprises the following steps:

(1) mixing the humectant A and glycerol uniformly in advance;

Example 3

Embodiment 3 provides an anti-aging polypeptide composition, which comprises 0.7 part of humectant A, 0.35 part of active polypeptide and 2 parts of swertiamarin.

The humectant A is sodium hyaluronate.

the molecular weight of the sodium hyaluronate macromolecules is 150 KDa.

The molecular weight of the molecule in the sodium hyaluronate is 340KDa W.

The molecular weight of the sodium hyaluronate micromolecules is 10 KDa.

The active polypeptide is an animal source extracellular matrix polypeptide.

the antioxidant is p-hydroxyacetophenone.

The preparation method of the anti-aging essence comprises the following steps:

(1) mixing the humectant A and glycerol uniformly in advance;

Example 4

Example 4 is similar to example 1 except that the sodium hyaluronate is free of sodium hyaluronate macromolecules.

Example 5

Example 5 is similar to example 1 except that the sodium hyaluronate is free of sodium hyaluronate mesomolecules.

Example 6

Example 5 is similar to example 1 except that the sodium hyaluronate is free of small sodium hyaluronate molecules.

Example 7

Example 7 is similar to example 1, except that the anti-aging polypeptide composition is devoid of bioactive peptides.

Example 8

Example 8 is similar to example 1, except that swertiamarin is absent from the anti-aging polypeptide composition.

Example 9

Example 9 is similar to example 1, except that the mass ratio of sodium hyaluronate to swertiamarin is 1: 1.

example 10

Example 10 is similar to example 1, except that the mass ratio of sodium hyaluronate to swertiamarin is 1: 15.

evaluation of Performance

1. And (3) stability testing: the anti-aging essences prepared in examples 1 to 10 were allowed to stand at room temperature for 30 days, and whether or not there was a delamination phenomenon was observed, and the results thereof are shown in table 1 below.

2. And (3) centrifugal test: the anti-aging essences prepared in examples 1 to 10 were centrifuged at 3000r/min for 30min to observe whether there was any delamination, and the results are shown in table 1 below.

TABLE 1 test data

	Stability of	Centrifugal stability
			Example 1	Without delamination	Without delamination
Example 2	Without delamination	Without delamination
			Example 3	Without delamination	Without delamination
Example 4	Layering	Layering
			Example 5	Layering	Layering
Example 6	Layering	Layering
			Example 7	Without delamination	Layering
Example 8	Without delamination	Without delamination
			Example 9	Without delamination	Layering
Example 10	Without delamination	Layering

3. And (3) anti-wrinkle efficacy test: seven testers were selected to apply the anti-aging essence prepared in example 1.

The test method comprises the following steps: all the testers performed cleansing treatment before sleeping at night, each person used the distributed moisturizing cream on the right face and the moisturizing cream on the left face, and the results are shown in the following table 2.

TABLE 2 test data

4. And (3) anti-wrinkle efficacy test: 50 of 25 to 50-year-old volunteers were randomly divided into 5 groups of 10 persons each, and the moisturizing and skin-caring creams prepared in examples 4 to 8 were used, respectively.

The test method comprises the following steps: all volunteers performed face cleaning treatment before sleeping every night, and each person used the distributed moisturizing cream on the right face and did not use the moisturizing cream on the left face. The trial period is one week, and the effective expression is that the left face and the right face of a trial user are obviously finer, whiter, smoother, less fine lines on the face, obviously lightened color spots, and are tender, white and more elastic; null means no change in the texture of the skin of the subject; the results are shown in table 3 below, where the skin quality of the test subjects was generally improved but the effect was not significant, ranging between effective and ineffective.

TABLE 3 test data

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims

1. An anti-aging polypeptide composition, which is characterized by comprising at least the following components in parts by weight: 0.2-0.7 part of humectant A, 0.03-0.35 part of active polypeptide and 0.5-2 parts of swertiamarin.

2. The polypeptide composition of claim 1, wherein the humectant A is selected from one or more of sodium hyaluronate, oat glucan, ceramide, and isopropyl myristate.

3. The polypeptide composition of claim 2, wherein the sodium hyaluronate is a combination of large sodium hyaluronate molecules, medium sodium hyaluronate molecules and small sodium hyaluronate molecules.

4. The anti-aging polypeptide composition of claim 1, wherein the active polypeptide is an extracellular matrix polypeptide of animal origin.

5. The anti-aging polypeptide composition as claimed in claim 4, wherein the preparation method of the extracellular matrix polypeptide of animal origin comprises the following steps:

(3) physically crushing the thawed tissue sample to obtain powder particles;

6. An anti-aging essence, which is prepared from the anti-aging polypeptide composition according to any one of claims 1 to 5.

7. The anti-aging essence according to claim 6, wherein the anti-aging essence comprises, by weight, 0.5-5 parts of a humectant B, 0.1-1 parts of an antioxidant, 0.5-4 parts of an anti-aging polypeptide composition, and water to 100 parts.

8. The anti-aging essence according to claim 7, wherein the moisturizer B is selected from one or more of glycerin, hexylene glycol, pentylene glycol, dipropylene glycol, butylene glycol, rose water, and propylene glycol.

9. The anti-aging essence according to claim 7, wherein the antioxidant is selected from one or more of tocopherol acetate, vitamin C, vitamin E, and p-hydroxyacetophenone.

10. The method for preparing the anti-aging essence according to any one of claims 6 to 9, wherein the method comprises the following steps:

(1) mixing the humectant A and glycerol uniformly in advance;