CN111066545A

CN111066545A - Method for preventing Duying transplantation ulcer disease

Info

Publication number: CN111066545A
Application number: CN202010002473.0A
Authority: CN
Inventors: 路秉翰; 谭广文; 殷明亮; 曾凤; 易慧琳; 黄玉; 刘晓洲
Original assignee: Guangzhou Pubang Landscape Architecture Co ltd; South China Agricultural University
Current assignee: Guangzhou Pubang Landscape Architecture Co ltd; South China Agricultural University
Priority date: 2020-01-02
Filing date: 2020-01-02
Publication date: 2020-04-28
Anticipated expiration: 2040-01-02
Also published as: CN111066545B

Abstract

The invention discloses a method for preventing a dulcamara transplantation ulcer disease. The pathogenic bacteria of the dioxin ulcer disease are identified to be ascomycota cryptotheca by a molecular technical means. Aiming at the way of canker, selecting and transplanting the unharmed Elaeagnus dulcis, reasonably mixing backfilled soil, planting a herbaceous host plant clover as an indicator plant, and properly adding No. 93 gasoline into the Pinxiukang and the Borax metalaxyl to ensure that the prevention and treatment effect is more remarkable. And moreover, the dark treatment can inhibit conidia, so that the probability of the occurrence of the Elaeagnus dulcis transplantation canker is reduced to below 5% from the original 55%, and the generation and the infection of the Elaeagnus dulcis canker are prevented through the ways of seedling lifting before planting, transplanting during planting, maintenance management after planting and the like.

Description

Method for preventing Duying transplantation ulcer disease

Technical Field

The invention belongs to the field of prevention of plant canker, and particularly relates to the field of prevention of Duying transplantation canker.

Background

Elaeocarpus dulcis (Elaeocarpus spp.) is a good local broad-leaved tree species in Guangdong province, and is one of the group-building species in mountain secondary forests in south China, because it is suitable for the climate in south China, and is therefore widely applied to urban garden construction. The Elaeagnus dulcis is luxuriant and emerald green in appearance, is an excellent tree species for landscaping, and has high ornamental value.

However, the stem part of the Elaeagnus dulcis is easy to suffer from diseases, and one or even a plurality of 'watermark' shaped disease spots appear on the stem or thicker branches; then the lesion site is swollen, enlarged and cracked to form typical ulcer spots; after a few months of disease, the disease spots surround the whole trunk, the diseased part is obviously sunken, and the upper tree crown is dry and dead. The Du Ying suffering from canker dies within one to two years, the disease spots of the survival plants are difficult to remove, and the survival plants can also have serious diseases. In the middle stage of the onset of the Duying canker, the lesion rapidly spreads just before and after the onset of the Duying sprout, from March to March when the temperature rises. From April to May, the disease basically stops moving when the Duying slips about 1 ruler. In May to June, spore horns are generated at the diseased part and are dispersed when meeting water, and conidia are spread along with wind and rain and are infected. From July to September, the disease develops again, and the disease stops moving in winter.

At present, the prevention report of the Duying canker is temporarily absent, the upper part of a dead plant after the disease is cut off in the prior art, but the branch of the lower part of a survival plant still has germs, so that the upper part of the dead plant can only be completely destroyed, and the larger economic loss is caused.

In light of the existing problems, the present invention has been made in an effort to develop a method for effectively preventing the transplanting ulcer disease of dulcamara.

Disclosure of Invention

The invention aims to provide an effective method for preventing the transplanting ulcer disease of dulcamara. The method mainly uses cultivation measures to reduce the use of chemical pesticides through researches on aspects of lifting seedlings before planting, transplanting during planting, maintenance management after planting and the like, infection ways of pathogenic bacteria and the like, thereby avoiding or reducing the occurrence of canker.

The technical scheme adopted by the invention is as follows:

in a first aspect of the invention, a method for preventing a dulcamara transplant ulcer disease is provided. According to an embodiment of the invention, the method comprises the steps of:

1) cutting off roots of the Chinese juniper trees, and spraying a growth promoter on the roots of the Chinese juniper trees;

2) spraying antitranspirant on the tree crowns, and carrying out dark treatment on the whole tree;

3) before planting, sterilizing the trees;

4) adjusting the pH value of the backfilled soil, and mixing the nitrogen fertilizer and the biocontrol bacteria;

5) transplanting the Elaeagnus dulcis to the planting hole, and compacting by using backfilled soil;

6) after planting, canker host indicator plants are planted along the projected edge of the crown.

According to an embodiment of the present invention, the phytopathogen is a crypthecodinium genus of ascomycota.

According to the embodiment of the invention, the juniper tree in the step 1) is 2-3 years old; the root cutting is step-by-step root cutting, namely, a trunk of the root is taken as a center, the excavation radius is used for drawing a circle, the circle is divided into 8-12 parts equally, the half is excavated for the first time at intervals, the rest part and the main root are cut off for the second time, and the radius of an earth digging ball is 6-8 times of the breast diameter of the trunk.

According to the embodiment of the invention, the growth promoter in the step 1) is naphthylacetic acid, GA3 gibberellin.

According to the embodiment of the invention, the anti-transpirant in the step 2) is a fast-activity forest transpirant, phenylmercuric acetate PMA and fulvic acid FA.

According to an embodiment of the present invention, the bactericide in step 3) is Pinxiukang and Boral metalaxyl; the concentration ratio of Pingxiukang to Bor metalaxyl is 850-950 ppm: 600 to 800 ppm.

According to an embodiment of the invention, the backfill soil of the step 4) is at least one of sulfur powder, mushroom fertilizer, wood dust, yellow loam and peat soil; the volume ratio of the sulfur powder to the mushroom fertilizer to the wood chips to the yellow loam to the peat soil is 1:1 (2-2.2), (4-4.6), (6-6.3); adjusting the pH value of the backfill soil to 5.0-5.5; the content of the nitrogen fertilizer is 15-25 g per plant; the biocontrol bacterium is bacillus subtilis.

According to the embodiment of the invention, the planting hole in the step 5) is 1-1.2 times of the thickness of the soil ball, and the diameter of the planting hole is 1.5-1.6 times of the diameter of the soil ball.

According to an embodiment of the present invention, the indicator plant in step 6) is clover, miscanthus sinensis.

According to the embodiment of the invention, the method also comprises periodic maintenance management, and comprises the following steps:

(1) one week after planting, pouring naphthylacetic acid and nutrient solution;

(2) after new leaves germinate, watering and applying a nitrogen-phosphorus-potassium compound fertilizer;

(3) spraying urea to the leaves 30-35 days after the sprouts grow out;

(4) spraying a bactericide on the whole tree; the bactericide is Pingxiukang, Borer metalaxyl and gasoline; the concentration ratio of Pingxiukang to Borer metalaxyl is 850-950 ppm: 600 to 800 ppm.

The backfill soil can inhibit the propagation of pathogens by mixing according to a specific proportion, and is beneficial to the rooting of the Elaeagnus dulcis.

850-950 ppm of Pingxiukang and 600-800 ppm of Borax and metalaxyl mixed solution are sprayed on the whole tree, No. 93 gasoline is added into the mixed medicament according to the proportion of 200:1, so that the medicament is better adsorbed on the surface of the leaf, the medicament effect is better after 14 days of medicament application, the action time of the medicament is prolonged, and the prevention and treatment effect is obvious.

Dark treatment, conidium inhibition: spore horns are generated in disease parts between 5 and 6 months, and conidia are spread along with wind and rain and invade the disease. And (4) dark treatment to inhibit conidia. Because the conidia of the Cryptotheca genus need illumination in production, the trunk is wrapped by the double-layer black shading net and the single-layer permeable geotextile 5-6 months per year, the production and the propagation of the conidia are reduced or avoided, and the occurrence of diseases is restrained from the source.

The invention has the beneficial effects that:

the pathogenic bacteria of the Duying canker are reported to belong to the genus of Ascomycota for the first time, the non-diseased Duying trees are selected and transplanted according to the way of the canker, the backfilled soil is mixed in a reasonable proportion, the herbaceous host plant clover is planted to be used as an indicating plant, and the Pinxiukang, the Borer and the metalaxyl are properly added with 93 gasoline, so that the prevention and treatment effect is more remarkable. And the dark treatment can inhibit conidium, so that the probability of the duvet transplantation canker is reduced to below 5 percent from the original 55 percent. Moreover, the cultivation method is mainly used, the use of a large amount of chemical reagents is reduced, a reference can be provided for the prevention or treatment of canker of other landscape garden plants, and an effective green prevention and control technology is provided.

Drawings

FIG. 1 trunk dip of Duying's ulcer disease in West Tung forest farm.

FIG. 2 Deutton canker symptoms in the Xigulin forest field bark splitting.

FIG. 3 death and dehiscence of Elaeagnus disease symptoms in the Tianxin forest farm.

FIG. 4 Deutton canker symptom death in the Tianxin forest farm.

FIG. 5 shows normal healthy plants of Elaeagnus dulcis.

FIG. 6 shows normal healthy plants of Elaeagnus dulcis.

Detailed Description

Reagent:

naphthylacetic acid was purchased from Anyang Quanfeng Biotechnology Co., Ltd (79-67), GA3 gibberellin was purchased from Henan Fuwei Biotechnology Co., Ltd (618-35), Kanghui forest antitranspirant was purchased from Wei high technology chemical Co., Ltd (58-468) in Henan, Phenylmercuric acid (PMA) was purchased from Jinkan commercial Co., Ltd (245-46), Fulvic Acid (FA) was purchased from Shanghai Yizheng Biotechnology Co., Ltd (479-66-3), Xiukang was purchased from Zhengzhong chemical product Co., Ltd (155-46), Bor metalaxyl was purchased from Jiangsu Longgu chemical Co., Ltd (55-46-1), NPK compound fertilizer was purchased from Zhengming chemical trade Co., Ltd (68-354), and HB101 phytol was purchased from Changning agricultural sales Co., Ltd (135-15).

FIGS. 1-4 show the Duying with ulcer disease in West-hole forest farm and Tianxin forest farm, respectively. Fig. 5 to 6 show normal dulcamara.

The following examples are given to further illustrate the embodiments of the present invention, but are not intended to limit the invention thereto.

Example 1:

the juniper primary producing area mountain forest and the juniper nursery at the transplanting place comprise the following steps:

1) cutting roots of 50 unharmed and 2-3-year-old dulcamara trees step by step, namely drawing a circle by using a root trunk as a center and an excavation radius, dividing the circle into 8 parts, excavating half for the first time, excavating at intervals, cutting the rest part and the main root for the second time, wherein the radius of an earth excavation ball is 6-8 times of the breast diameter of the trunk; 600ppm of naphthylacetic acid growth promoter is sprayed on the roots of the trees.

2) Spraying 1:20 fast-living forest antitranspirant on the tree crown, covering the whole tree with a sunshade net for dark treatment and spray cooling.

3) Before planting, 850ppm of Pingxiukang and 650ppm of Borax metalaxyl are used as mixed bactericides, and the mixture is sprayed on the whole trees containing soil balls.

4) Mixing sulfur powder, mushroom fertilizer, wood chips, yellow loam and peat soil according to the proportion of 1:1:2:4:6 proportion to prepare backfill soil, adjusting the pH value of the backfill soil to 5.4, and then mixing 15g of nitrogen fertilizer and biocontrol bacteria (bacillus subtilis).

5) The planting hole is 1-1.2 times of the thickness of the soil ball, the diameter is 1.5-1.6 times of the diameter of the soil ball, and the bottom of the planting hole is concave. The concave groove is 20cm long, 20cm wide and 10-15cm deep. And arranging a water storage tank at the lower side of the topography at the bottom of the planting hole, digging a water storage tank with the depth of 20cm and the width of 20cm along the edge of the hole, filling broken stones, arranging a D100mm polyethylene threaded pipe in the water storage tank, opening a hole on the surface, and wrapping a layer of non-woven fabric. Paving 10cm of broken stone at the bottom of the planting hole, and then paving a layer of 200g/m²The non-woven fabric is not filled with broken stones in the concave groove at the center of the bottom of the hole, the non-woven fabric is not laid on the top surface, and the concave groove is backfilled with mixed backfill soil and is continuously backfilled to 10cm high.

Cutting off soil balls for binding, uniformly placing root systems of the seedlings in the planting holes to ensure that the root systems are spread, backfilling mixed backfilled soil for 30-40cm, compacting and compacting the backfilled soil, and enabling the top surfaces of the planting holes to be flush with the ground. And immediately pouring root water after cofferdam, wherein the water is required to be completely poured, and the mud is expressed until the mud is wet.

6) After planting, planting the host clover of the canker pathogenic bacteria grass with the width of 0.5-1 m (preventing pathogenic bacteria from infecting the trunk of the Elaeagnus dulcis) along the periphery of the projection edge of the crown as an indication plant. Removing the indicating plants and burning the indicating plants intensively between 12 months and 1 month in the next year, and spraying 650ppm Bor-metalaxyl to sterilize the soil.

Because the host plant is a herbaceous plant, the plant is more susceptible to diseases than woody Elaeagnus dulcis. When the host plant is infected, the pesticide is sprayed to prevent and control the Chinese dulcamara immediately, the clovers are removed and burnt intensively, and 650ppm of Borax metalaxyl is sprayed to kill bacteria on the soil.

7) And (3) regular maintenance management: after transplanting and fixing, watering times in a maintenance period by taking soil cracks on the surface of a soil ball as watering intervals, and watering 650ppm of naphthylacetic acid rooting water and nutrient solution (HB101 phytochemicals) one week after planting; 30 days after the big tree sprouts new leaves, a nitrogen-phosphorus-potassium compound fertilizer with the concentration of 0.2 to 0.5 percent is applied in combination with watering; dissolving urea into water at a ratio of 1:200 30 days after sprouting, and spraying leaves 17-19 days in sunny days; spraying 850ppm of Pinxiukang and 600ppm of Bor and metalaxyl mixed bactericide from 1-2 middle of month and 5-6 middle of month each year, wherein No. 93 gasoline is added into the mixed pesticide according to the volume ratio of 200: 1; and (4) dark treatment to inhibit conidia. Spore horns are generated in disease parts between 5 and 6 months, and conidia are spread along with wind and rain and invade the disease. Because the conidia of the Cryptotheca genus need illumination in production, the trunk is wrapped by a double-layer black shading net and a single-layer permeable geotextile (90% dark treatment) in 5-6 months every year, and the production and the propagation of the conidia are reduced.

As a result: the canker condition of the trees is checked after the trees are transplanted for one year, 50 trees are transplanted to survive for 48 trees, the death reason is dry trunks, canker occurs for 2 trees, the occurrence reason is that the trunks of the Elaeagnus dulcis have holes, the growth vigor is poor, and the incidence rate of the canker is 4%.

Example 2:

the method for transplanting the mountain forest of the native place of dulcamara to garden engineering comprises the following steps:

1) cutting roots of 60 non-diseased Elaeagnus dulcis trees of 2-3 years old step by step, namely drawing a circle by taking a trunk of the root as a center and an excavation radius, dividing the circle into 8 parts, excavating half for the first time at intervals, excavating for the second time, and cutting the rest part and the main root for the second time, wherein the radius of an earth excavation ball is 6-8 times of the breast diameter of the trunk; 600ppm of GA3 gibberellin growth promoter is sprayed on the roots of the trees.

2) Spraying 1:20 fast-living forest antitranspirant on the crown of the tree, covering the whole tree with a sunshade net and cooling by spraying.

3) Before planting, 900ppm of Pingxiukang and 600ppm of Borax metalaxyl are used as mixed bactericides, and the mixture is sprayed on the whole trees containing soil balls.

4) Mixing sulfur powder, mushroom fertilizer, wood chips, yellow loam and peat soil according to the proportion of 1:1: 2.2: 4.6: 6.3, adjusting the pH value of the backfill soil to 5.4, and then mixing 15g of nitrogen fertilizer and biocontrol bacteria (bacillus subtilis).

5) The planting hole is 1-1.2 times of the thickness of the soil ball, the diameter of the planting hole is 1.5-1.6 times of the diameter of the soil ball, and the bottom of the planting hole is of a concave shape. The concave groove is 20cm long, 20cm wide and 15cm deep;

transplanting the dulcamara to the concave planting hole, arranging a water storage tank, filling broken stones in the water storage tank, arranging a polyethylene threaded pipe in the tank, forming a hole on the surface of the polyethylene threaded pipe, and wrapping a layer of non-woven fabric outside the polyethylene threaded pipe; compacting and compacting the backfilled undisturbed soil until the backfilled undisturbed soil is level to the ground, and pouring the fixed root water until the mud surface is wet.

6) After planting, planting the host clover of the canker pathogenic bacteria grass with the width of 0.5-1 m (preventing pathogenic bacteria from infecting the trunk of the Elaeagnus dulcis) along the periphery of the projection edge of the crown as an indication plant. Removing the indicating plants and burning the indicating plants intensively between 12 months and 1 month in the next year, and spraying 800ppm Bor-metalaxyl to the soil for sterilization.

7) And (3) regular maintenance management: after transplanting and fixing, watering times in a maintenance period by taking soil cracks on the surface of a soil ball as watering intervals, and watering 650ppm of naphthylacetic acid rooting water and nutrient solution again one week after planting; after the big tree sprouts new leaves for 35 days, a nitrogen-phosphorus-potassium compound fertilizer with the concentration of 0.4 percent is applied in combination with watering; dissolving urea into water at a ratio of 1:200 30 days after sprouting, and spraying leaves 17-19 days in sunny days; spraying 850ppm of Pingxiukang and 800ppm of Bor and metalaxyl mixed bactericide from 1-2 middle of month and 5-6 middle of month each year, wherein No. 93 gasoline is added into the mixed pesticide according to the volume ratio of 200: 1; and 5-6 months every year, the trunk is wrapped by a double-layer black shading net and a single-layer permeable geotextile so as to reduce the spread of pathogenic bacteria conidia.

As a result: the canker condition of the trees is checked after the trees are transplanted for one year, 57 survivors are transplanted for 60 trees, 3 canker diseases occur due to dry trunks, the holes are formed in the trunks of the Elaeagnus dulcis, and the incidence rate of the canker diseases is 5%.

Comparative example 1

under the same conditions as in example 1, sulfur powder, mushroom fertilizer, wood chips, yellow loam and peat soil were mixed in a ratio of 1:1:1:4:7 to prepare backfill soil, the pH of the backfill soil was adjusted to 5.4, and then 15g of nitrogen fertilizer was mixed.

Comparative example 2

under the same conditions as in example 1, sulfur powder, mushroom fertilizer, wood chips, yellow loam and peat soil were mixed in a ratio of 1:1:3:4:5 to prepare backfill soil, the pH of the backfill soil was adjusted to 5.4, and then 15g of nitrogen fertilizer was mixed.

Comparative example 3

under the same conditions as in example 1, sulfur powder, mushroom fertilizer, wood chips, yellow loam and peat soil were mixed in a ratio of 1:1:1:4:4 to prepare backfill soil, the pH of the backfill soil was adjusted to 5.4, and then 15g of nitrogen fertilizer was mixed.

Comparative example 4

under the same conditions as in example 1, sulfur powder, mushroom fertilizer, wood chips, yellow loam and peat soil were mixed in a ratio of 1:1:2:4:3 to prepare backfill soil, the pH of the backfill soil was adjusted to 5.4, and then 15g of nitrogen fertilizer was mixed.

Comparative example 5

otherwise, the same conditions as in example 1 were applied, unmodified conventional planting soil was added, and then 15g of nitrogen fertilizer was mixed.

The results are shown in Table 1:

TABLE 1 inhibitory Effect of different backfill formulations on ulcer disease pathogenic bacteria

The letters abcd in the table represent significant differences between the various values.

As can be seen from Table 1, when the ratio of the sulfur powder to the mushroom fertilizer to the wood chips to the yellow loam to the peat soil is 1:1:2:4:6, the inhibition rate on the growth of hyphae is highest, the inhibition rate on the spore yield and the spore germination are also highest, and the inhibition effect is obvious compared with a control, which indicates that the backfill soil has a better inhibition effect on the pathogenic bacteria of the Elaeagnus dulcis ulcer disease and effectively inhibits the occurrence of the disease.

TABLE 2 influence of different backfill proportions on root growth of Elaeagnus dulcis

It can be seen from table 2 that when the ratio of sulfur powder, mushroom fertilizer, wood dust, yellow loam and peat soil is 1:1:2:4:6, the rooting rate, average root length and average root diameter are all the highest, and the study shows that under the condition, the backfill soil formula has a significant promotion effect on the rooting of the Elaeagnus dulcis.

Comparative example 6

The mountain forest of the native place of Duying is transplanted to garden engineering, other conditions are the same as the example 1, and the host maidenhair of canker pathogenic bacteria grass is planted around the projection edge of the crown, the width is 0.5-1 m, (the pathogenic bacteria are prevented from infecting the Duying trunk), and the host maidenhair is used as an indicator plant. And (3) clearing the indicating plants and burning the indicating plants intensively between 12 months and 1 month in the next year, and spraying 600-800 ppm of Borax metalaxyl to the soil for sterilization. Because the host plant is a herbaceous plant, the plant is more susceptible to diseases than woody Elaeagnus dulcis. When the host plant is infected, the pesticide is sprayed to prevent and treat the dulcamara immediately, the maidenhair is removed and burnt intensively, and 650ppm of Borax metalaxyl is sprayed to kill the germs on the soil (as shown in Table 3).

Comparative example 7

The mountain forest of the native place of Duying is transplanted to garden engineering, other conditions are the same as the example 1, the host pennisetum alopecuroides of the canker pathogenic bacteria grass is planted around the projection edge of the crown, the width is 0.5-1 m, (the pathogenic bacteria are prevented from infecting the Duying trunk), and the host pennisetum alopecuroides is used as an indicator plant. And (3) clearing the indicating plants and burning the indicating plants intensively between 12 months and 1 month in the next year, and spraying 600-800 ppm of Borax metalaxyl to the soil for sterilization. Because the host plant is a herbaceous plant, the plant is more susceptible to diseases than woody Elaeagnus dulcis. When the host plant is infected, the pesticide is sprayed to prevent and control the Chinese gooseberry, the Chinese gooseberry is removed and burnt intensively, and 650ppm of Borax metalaxyl is sprayed to kill bacteria on the soil.

Comparative example 8

The mountain forest of the native place of Duying is transplanted to garden engineering, other conditions are the same as the example 1, and the host Miscanthus sinensis of the canker pathogenic bacteria grass is planted around the projection edge of the crown, the width is 0.5-1 m, (the pathogenic bacteria are prevented from infecting the Duying trunk), and the Miscanthus sinensis serves as an indicator plant. And (3) clearing the indicating plants and burning the indicating plants intensively between 12 months and 1 month in the next year, and spraying 600-800 ppm of Borax metalaxyl to the soil for sterilization. Because the host plant is a herbaceous plant, the plant is more susceptible to diseases than woody Elaeagnus dulcis. When the host plant is infected, the pesticide is sprayed to prevent and treat the Elaeagnus dulcis immediately, the Miscanthus sinensis is removed and burnt intensively, and 650ppm of Borl and metalaxyl are sprayed to kill bacteria on the soil.

Comparative example 9

The mountain forest of the native place of production of the Elaeagnus dulcis is transplanted to garden engineering, other conditions are the same as the example 1, the host of the pathogenic bacteria grass of the canker disease is the Carex aurantiaca, the width is 0.5-1 m, and the plants are used as indicating plants, wherein the host is the Carex aurantiaca (the width is 0.5-1 m) and the plants can prevent the pathogenic bacteria from infecting the trunk of the Elae. And (3) clearing the indicating plants and burning the indicating plants intensively between 12 months and 1 month in the next year, and spraying 600-800 ppm of Borax metalaxyl to the soil for sterilization. Because the host plant is a herbaceous plant, the plant is more susceptible to diseases than woody Elaeagnus dulcis. When the host plant is infected, the pesticide is sprayed to the Elaeagnus dulcis immediately to prevent and control the Elaeagnus dulcis, the Elaeagnus dulcis is removed and burnt intensively, and 650ppm Borer and metalaxyl are sprayed to soil to sterilize.

TABLE 3 infection of various hosts by the pathogenic bacteria of Elaeagnus dulcis Rolfe

From table 3, it can be seen that clover has the highest incidence and the highest disease value, thus indicating that clover is more easily infected by the pathogenic bacteria, and is most suitable for being planted at the projected edge of the crown of the Elaeagnus dulcis as an indicator plant.

Comparative example 10

Under the same conditions as in example 1, Pingxikukang (Table 4) was used as a mixed bactericide for periodic maintenance management.

Comparative example 11

Under the same conditions as in example 1, Borax metalaxyl was used as the mixed fungicide for the periodic maintenance management.

Comparative example 12

Under the same conditions as in example 1, Pingxukang + Borl metalaxyl was used as the mixed fungicide for the periodic maintenance management.

Comparative example 13

Other conditions were the same as in example 1, and no bactericide was used for the periodic maintenance management.

TABLE 4 preventive and therapeutic effects of the drugs on Elaeagnus dulcis ulcer

Comparative example 14

Other conditions were the same as in example 1, and the periodic maintenance management was performed in 30% darkness for 5 to 6 months per year (Table 5).

Comparative example 15

Other conditions are the same as those in example 1, and the periodic maintenance management is performed by performing 60% dark treatment every 5-6 months.

Comparative example 16

Other conditions are the same as those in the embodiment 1, and the periodical maintenance management is carried out for 5-6 months per year without carrying out dark treatment and directly irradiating by using a fluorescent lamp.

TABLE 5 inhibitory Effect of dark treatment on pathogenic bacteria

Note: the data in the table are the average of 3 replicates, with different lower case letters in the same column indicating significant differences at the 0.05 level.

As can be seen from Table 1, the treatment with T3 showed the best inhibitory effect on hyphae, spore yield and spores, and the inhibitory effect was the best with increasing darkness. The research shows that the dark treatment has an inhibiting effect on the propagation of pathogenic bacteria of the Elaeagnus dulcis canker, the generation of conidium can be reduced or avoided, and the occurrence of diseases can be inhibited from the source.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. A method for preventing Duying transplantation canker is characterized by comprising the following steps:

3) before planting, sterilizing the trees;

6) after planting, the host indicator plant of canker is planted at the projection edge along the crown.

2. The method of claim 1, wherein the phytopathogen is a cryptotheca of the phylum ascomycota.

3. The method of claim 1, wherein the step 1) root-breaking method is: and (3) cutting off the root step by step, namely, taking the trunk of the root as the center, drawing a circle by the excavation radius, dividing the circle into 8-12 parts, excavating half for the first time at intervals, excavating for the second time, cutting off the rest part and the main root, wherein the radius of the soil excavation ball is 6-8 times of the breast diameter of the trunk.

4. The method as claimed in claim 1, wherein the growth promoter of step 1) is naphthylacetic acid, GA3 gibberellin.

5. The method according to claim 1, wherein the anti-transpirant in step 2) is fast-activity forest transpirant, phenylmercuric acetic acid, fulvic acid.

6. The method according to claim 1, wherein the bactericide in step 3) is Pingxukang and Boral metalaxyl; the concentration ratio of Pingxiukang to Bor metalaxyl is 850-950 ppm: 600 to 800 ppm.

7. The method according to claim 1, wherein the backfill soil of step 4) is at least one of sulfur powder, mushroom manure, wood chips, yellow loam, peat soil; the volume ratio of the sulfur powder to the mushroom fertilizer to the wood chips to the yellow loam to the peat soil is 1:1 (2-2.2), (4-4.6) and (6-6.3).

8. The method of claim 1, wherein the backfill soil is adjusted to a pH of 5.0 to 5.5; the content of the nitrogen fertilizer is 15-25 g per plant; the biocontrol bacterium is bacillus subtilis.

9. The method of claim 1, wherein the indicator plant of step 6) is Trifolium pratense, Miscanthus floridulus.

10. The method of claim 1, further comprising periodic maintenance management, wherein the periodic maintenance management steps are as follows:

1) one week after planting, pouring naphthylacetic acid and nutrient solution;

2) after new leaves germinate, watering and applying nitrogen phosphorus potassium compound fertilizer;

3) spraying urea to the leaves 30-35 days after the sprouts grow out;

4) spraying a bactericide on the whole tree; the bactericide is Pingxiukang, Borer metalaxyl and gasoline.