CN106613629A - Seedling method for elaeocarpusdecipiens - Google Patents

Seedling method for elaeocarpusdecipiens Download PDF

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Publication number
CN106613629A
CN106613629A CN201610960005.8A CN201610960005A CN106613629A CN 106613629 A CN106613629 A CN 106613629A CN 201610960005 A CN201610960005 A CN 201610960005A CN 106613629 A CN106613629 A CN 106613629A
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elaeocarpus decipiens
seedling
seed
weight
elaeocarpus
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余安芹
苏丹青
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Bengbu Jinniu Agricultural Science And Technology Development Co Ltd
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Bengbu Jinniu Agricultural Science And Technology Development Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a seedling method for elaeocarpusdecipiens which comprises the following steps: (1) a seed is stored and germinated after a treatment process ; (2) a seedling is transplanted from a substrate; (3) a cultivated field is fertilized and plowed and a spacing between rows is determined. (4) The seedlings, with nutrient solution of plant roots, are transplanted with soil clod and then a nurturing management is carried out. The seedling method for elaeocarpusdecipiens, by a mixture of the seed of pit stored and the uniform and robust seedlings from plastic bag containing, has the advantages of reducing a disease incidence in a seedling stage which can lay a foundation for transplant seedling. The plant roots of transplanted seedling with nutrient solution are capable of improving a survival rate of. The seedling method for elaeocarpusdecipiens has the advantages that the method is simple, material source is wide, the cost is low, and a seedling amount is big, so as to a planting income can be improved .

Description

A kind of method for culturing seedlings of Elaeocarpus decipiens
Technical field
The present invention relates to technology seedling growth Cultivating techniques field, more particularly to a kind of method for culturing seedlings of Elaeocarpus decipiens.
Background technology
Elaeocarpus decipiens, the false Fructus Myricae rubrae of another name, prunus mume (sieb.) sieb.et zucc. wipe meal, Fructus Canarii, wild olive, the tree of gallbladder eight, Fructus Canarii albi, edge lobe Elaeocarpus decipiens.
Elaeocarpaceae Elaeocarpus aiphyllium, high up to 15 meters, there is little cognate at leaf keratin, lanceolar or lanceolar, edge; Petiole has at the beginning microtriche, and deglabration is net when solid.Axil being born in raceme, rachises are very thin, and floral white, sepal drapes over one's shoulders pin more Shape, petal obovate, flower pesticide top is without appurtenance;Drupe is oval, and outer peel is without hair, endocarp heavily fortified point sclerotin, the month at florescence 6-7.
The evergreen quick growing species of trees of Elaeocarpus decipiens, material is good, and strong adaptability, pest and disease damage is few.Be garden view and admire and turn the four sides green it is excellent Kind.It is distributed in Guangxi, Guangdong, Jiangxi, Fujian, Taiwan, Zhejiang;Vietnam, in raw low mountain mountain valley woods.
English likes warm moist environment, and tolerance to cold is slightly worse.Slightly shade tolerant, well developed root system, rudiment power is strong, resistance to pruning.Happiness draining is good Good, moistening, fertile acid ground.Suitable yellow earth and the red-yellow soil mountain area for being born in acidity, if in Plain plant, it is necessary to which draining is good Good, the speed of growth is medium fast.To sulfur dioxide resistance.
To realize that Elaeocarpus decipiens seeds can grow in the Yangtze river basin, more nursery stock species are provided for market, it is another for increase nursery stock Income, the high method for culturing seedlings of the high-survival rate of the open present invention of spy, plantation income.
The content of the invention
In order to overcome the above-mentioned deficiencies of the prior art, the invention provides a kind of method for culturing seedlings of Elaeocarpus decipiens.
The technical solution adopted in the present invention is:
A kind of method for culturing seedlings of Elaeocarpus decipiens, step includes as follows:
(1) harvesting ripe Elaeocarpus decipiens fruit, is 10 by itself and the clever shell of Semen Tritici aestivi, Baijunling by weight:5:0.02 be mixed after carry out heap Put and macerate rotten 5-7 days, after then being air-dried with water elutriation removal peel and Semen Tritici aestivi grain husk shell placement ventilation, Elaeocarpus decipiens seed and adjuvant are entered Row mixing carries out cellar storage, and it is 33-38% to control the water content of compound, the mixing weight of described Elaeocarpus decipiens seed and adjuvant Amount is than being 1:1, sealing cellar storage grows tender shoots to the Elaeocarpus decipiens seed broken shell for having 40-50% months in next year 2;
(2) in the booth in month in spring 2, nursery is carried out with bag containing soilless substrate, each bag containing soilless substrate is placed the Elaeocarpus decipiens after 1-2 grains store sth. in a cellar and broken Shell seed bud, and coating 3-4mm thick hypothalluses, the substrate in the bag containing soilless substrate by peat, Vermiculitum, plant ash, decomposed manure, 50% sulfur suspending agent, by its weight 13 are compared:14:8:6:0.01 mixing is formed, and its water content 40-45% treats that sprout exposes substrate After pour time permeable, and control temperature of shed, on 22-28 DEG C of daytime, at 18-22 DEG C of night, per time slow water is poured within 5-7 days, wait seedling length extremely High 31-42cm can be transplanted;
(3) in the winter time December to transplanting carries out ploughing deeply 34-40cm, to transplanting every then in month in spring 2, the last ten-days period Mu applies the 33-36cm that turns over after decomposed manure 1800-2000kg, applies N15, P15, K15 to the first mu in transplanting ground before transplanting and answers The 30-33cm that turns over is carried out after Hefei 45-50kg and carbamide 40-50kg, and makees a width of 50-60cm furrow, a height of 30-35cm, furrow ditch Wide 20-25cm, and make the kind plant hole that a diameter of 20-25cm, depth are 10-15cm on furrow;
(4) the Elaeocarpus decipiens seedling in step (2) is first removed after bag containing soilless substrate, band soil libation at an ancient wedding ceremony nutritional solution B is transplanted in step (3) and transplants ground Kind plant hole in banket, then pour permeable, carry out middle weeding, and carry out dry antiperspirant immediately to water, waterlogging caused by excessive rain drainage works, together When carry out at rainy season pest and disease damage prevention daily management.
Further, the adjuvant described in step (1) is made up of fine sand, clever shell, herb liquid, nutritional solution, and it prepares weight ratio Example is 20:8:0.1:0.1;Its preparation steps is:First herb liquid is diluted to into 200 times of liquid carries out being sprayed to its table to Elaeocarpus decipiens seed Face it is micro- it is wet after, mix homogeneously with fine sand, clever shell and nutritional solution.
Further, nutritional solution A is 1 by weight:1:2:2:8 brassin lactones, indolebutyric acid, compound sodium nitrophenolate, amine are fresh Ester and ethanol are mixed.
Further, the spacing of the kind plant hole described in step (3) is 40-50cm.
Further, the nutritional solution B described in step (5) by Bombyx mori L., brassin lactones, compound sodium nitrophenolate, cattle manure by weight For 0.1:0.2:0.2:5 mixings are formed.
Beneficial effects of the present invention:The Seedling that the seed and bag containing soilless substrate that blended material stores sth. in a cellar is educated is healthy and strong together, reduces children in canopy The disease incidence in seedling stage, is that replanting and culturing is laid a solid foundation, in addition to the process of transplanting seedling root libation at an ancient wedding ceremony nutritional solution, to improve into Motility rate, the method that the present invention is adopted is simple, and materials source is wide, and low cost, output of seedling is big, to improve plantation income.
Specific embodiment
Embodiment 1
A kind of method for culturing seedlings of Elaeocarpus decipiens, step includes as follows:
(1) harvesting ripe Elaeocarpus decipiens fruit, is 10 by itself and the clever shell of Semen Tritici aestivi, Baijunling by weight:5:0.02 be mixed after carry out heap Put and macerate rotten 5 days, after then being air-dried with water elutriation removal peel and Semen Tritici aestivi grain husk shell placement ventilation, Elaeocarpus decipiens seed is carried out with adjuvant Mixing carries out cellar storage, and it is 33-35% to control the water content of compound, the mixed weight of described Elaeocarpus decipiens seed and adjuvant Than for 1:1, sealing cellar storage grows tender shoots to the Elaeocarpus decipiens seed broken shell for having 40-43% months in next year 2;
(2) in the booth in month in spring 2, nursery is carried out with bag containing soilless substrate, each bag containing soilless substrate is placed the Elaeocarpus decipiens after 1-2 grains store sth. in a cellar and broken Shell seed bud, and coating 3-4mm thick hypothalluses, the substrate in the bag containing soilless substrate by peat, Vermiculitum, plant ash, decomposed manure, 50% sulfur suspending agent, by its weight 13 are compared:14:8:6:0.01 mixing is formed, and its water content 40-42% treats that sprout exposes substrate After pour time permeable, and control temperature of shed, on 22-24 DEG C of daytime, at 18-22 DEG C of night, time slow water was poured per 5 days, wait seedling length paramount 31-35cm can be transplanted;
(3) in the winter time December to transplanting carries out ploughing deeply 34-36cm, to transplanting every then in month in spring 2, the last ten-days period Mu applies the 33-34cm that turns over after decomposed manure 1800kg, applies the compound fertilizer of N15, P15, K15 to transplanting the first mu in ground before transplanting The 30-31cm that turns over is carried out after 45-46kg and carbamide 40-42kg, and makees a width of 50-53cm furrow, a height of 30-32cm, furrow ditch width 20- 22cm, and make the kind plant hole that a diameter of 20-22cm, depth are 10-12cm on furrow;
(4) the Elaeocarpus decipiens seedling in step (2) is first removed after bag containing soilless substrate, band soil libation at an ancient wedding ceremony nutritional solution B is transplanted in step (3) and transplants ground Kind plant hole in banket, then pour permeable, carry out middle weeding, and carry out dry antiperspirant immediately to water, waterlogging caused by excessive rain drainage works, together When carry out at rainy season pest and disease damage prevention daily management.
Further, the adjuvant described in step (1) is made up of fine sand, clever shell, herb liquid, nutritional solution, and it prepares weight ratio Example is 20:8:0.1:0.1;Its preparation steps is:First herb liquid is diluted to into 200 times of liquid carries out being sprayed to its table to Elaeocarpus decipiens seed Face it is micro- it is wet after, mix homogeneously with fine sand, clever shell and nutritional solution.
Further, nutritional solution A is 1 by weight:1:2:2:8 brassin lactones, indolebutyric acid, compound sodium nitrophenolate, amine are fresh Ester and ethanol are mixed.
Further, the spacing of the kind plant hole described in step (3) is 40-42cm.
Further, the nutritional solution B described in step (5) by Bombyx mori L., brassin lactones, compound sodium nitrophenolate, cattle manure by weight For 0.1:0.2:0.2:5 mixings are formed.
Embodiment 2
A kind of method for culturing seedlings of Elaeocarpus decipiens, step includes as follows:
(1) harvesting ripe Elaeocarpus decipiens fruit, is 10 by itself and the clever shell of Semen Tritici aestivi, Baijunling by weight:5:0.02 be mixed after carry out heap Put and macerate rotten 6 days, after then being air-dried with water elutriation removal peel and Semen Tritici aestivi grain husk shell placement ventilation, Elaeocarpus decipiens seed is carried out with adjuvant Mixing carries out cellar storage, and it is 34-36% to control the water content of compound, the mixed weight of described Elaeocarpus decipiens seed and adjuvant Than for 1:1, sealing cellar storage grows tender shoots to the Elaeocarpus decipiens seed broken shell for having 44-47% months in next year 2;
(2) in the booth in month in spring 2, nursery is carried out with bag containing soilless substrate, each bag containing soilless substrate is placed the Elaeocarpus decipiens after 1-2 grains store sth. in a cellar and broken Shell seed bud, and coating 3-4mm thick hypothalluses, the substrate in the bag containing soilless substrate by peat, Vermiculitum, plant ash, decomposed manure, 50% sulfur suspending agent, by its weight 13 are compared:14:8:6:0.01 mixing is formed, and its water content 43-44% treats that sprout exposes substrate After pour time permeable, and control temperature of shed, on 24-26 DEG C of daytime, at 19-20 DEG C of night, time slow water was poured per 6 days, wait seedling length paramount 36-38cm can be transplanted;
(3) in the winter time December to transplanting carries out ploughing deeply 36-38cm, to transplanting every then in month in spring 2, the last ten-days period Mu applies the 34-35cm that turns over after decomposed manure 1900kg, applies the compound fertilizer of N15, P15, K15 to transplanting the first mu in ground before transplanting The 32cm that turns over is carried out after 47-48kg and carbamide 46-48kg, and makees a width of 54-57cm furrow, a height of 33-34cm, furrow ditch width 23- 24cm, and make the kind plant hole that a diameter of 23-24cm, depth are 13-14cm on furrow;
(4) the Elaeocarpus decipiens seedling in step (2) is first removed after bag containing soilless substrate, band soil libation at an ancient wedding ceremony nutritional solution B is transplanted in step (3) and transplants ground Kind plant hole in banket, then pour permeable, carry out middle weeding, and carry out dry antiperspirant immediately to water, waterlogging caused by excessive rain drainage works, together When carry out at rainy season pest and disease damage prevention daily management.
Further, the adjuvant described in step (1) is made up of fine sand, clever shell, herb liquid, nutritional solution, and it prepares weight ratio Example is 20:8:0.1:0.1;Its preparation steps is:First herb liquid is diluted to into 200 times of liquid carries out being sprayed to its table to Elaeocarpus decipiens seed Face it is micro- it is wet after, mix homogeneously with fine sand, clever shell and nutritional solution.
Further, nutritional solution A is 1 by weight:1:2:2:8 brassin lactones, indolebutyric acid, compound sodium nitrophenolate, amine are fresh Ester and ethanol are mixed.
Further, the spacing of the kind plant hole described in step (3) is 46-48cm.
Further, the nutritional solution B described in step (5) by Bombyx mori L., brassin lactones, compound sodium nitrophenolate, cattle manure by weight For 0.1:0.2:0.2:5 mixings are formed.
Embodiment 3
A kind of method for culturing seedlings of Elaeocarpus decipiens, step includes as follows:
(1) harvesting ripe Elaeocarpus decipiens fruit, is 10 by itself and the clever shell of Semen Tritici aestivi, Baijunling by weight:5:0.02 be mixed after carry out heap Put and macerate rotten 7 days, after then being air-dried with water elutriation removal peel and Semen Tritici aestivi grain husk shell placement ventilation, Elaeocarpus decipiens seed is carried out with adjuvant Mixing carries out cellar storage, and it is 36-38% to control the water content of compound, the mixed weight of described Elaeocarpus decipiens seed and adjuvant Than for 1:1, sealing cellar storage grows tender shoots to the Elaeocarpus decipiens seed broken shell for having 48-50% months in next year 2;
(2) in the booth in month in spring 2, nursery is carried out with bag containing soilless substrate, each bag containing soilless substrate is placed the Elaeocarpus decipiens after 1-2 grains store sth. in a cellar and broken Shell seed bud, and coating 3-4mm thick hypothalluses, the substrate in the bag containing soilless substrate by peat, Vermiculitum, plant ash, decomposed manure, 50% sulfur suspending agent, by its weight 13 are compared:14:8:6:0.01 mixing is formed, and its water content 44-45% treats that sprout exposes substrate After pour time permeable, and control temperature of shed, on 26-28 DEG C of daytime, at 20-22 DEG C of night, time slow water was poured per 7 days, wait seedling length paramount 40-42cm can be transplanted;
(3) in the winter time December to transplanting carries out ploughing deeply 38-40cm, to transplanting every then in month in spring 2, the last ten-days period Mu applies the 35-36cm that turns over after decomposed manure 2000kg, applies the compound fertilizer of N15, P15, K15 to transplanting the first mu in ground before transplanting The 32-33cm that turns over is carried out after 48-50kg and carbamide 48-50kg, and makees a width of 58-60cm furrow, a height of 34-35cm, furrow ditch width 24- 25cm, and make the kind plant hole that a diameter of 24-25cm, depth are 14-15cm on furrow;
(4) the Elaeocarpus decipiens seedling in step (2) is first removed after bag containing soilless substrate, band soil libation at an ancient wedding ceremony nutritional solution B is transplanted in step (3) and transplants ground Kind plant hole in banket, then pour permeable, carry out middle weeding, and carry out dry antiperspirant immediately to water, waterlogging caused by excessive rain drainage works, together When carry out at rainy season pest and disease damage prevention daily management.
Further, the adjuvant described in step (1) is made up of fine sand, clever shell, herb liquid, nutritional solution, and it prepares weight ratio Example is 20:8:0.1:0.1;Its preparation steps is:First herb liquid is diluted to into 200 times of liquid carries out being sprayed to its table to Elaeocarpus decipiens seed Face it is micro- it is wet after, mix homogeneously with fine sand, clever shell and nutritional solution.
Further, nutritional solution A is 1 by weight:1:2:2:8 brassin lactones, indolebutyric acid, compound sodium nitrophenolate, amine are fresh Ester and ethanol are mixed.
Further, the spacing of the kind plant hole described in step (3) is 48-50cm.
Further, the nutritional solution B described in step (5) by Bombyx mori L., brassin lactones, compound sodium nitrophenolate, cattle manure by weight For 0.1:0.2:0.2:5 mixings are formed.
By above-described embodiment, table 1 below conclusion is obtained:
Table 1
Accelerating germination rate Seedling percent Seedling growth Transplanting survival rate
Embodiment 1 90% 96% Seedling is healthy and strong together, well developed root system 95%
Embodiment 2 88% 92% Seedling is healthy and strong together, well developed root system 92%
Embodiment 3 92% 94% Seedling is healthy and strong together, well developed root system 95%
Conventional method 71% 80-85% Growing way is uneven, and root hair is few 85%
Can be obtained by data above, the Seedling that the seed and bag containing soilless substrate that blended material stores sth. in a cellar is educated is healthy and strong together, reduce Seedling Stage in canopy Disease incidence, be that replanting and culturing is laid a solid foundation, in addition to transplant seedling root libation at an ancient wedding ceremony nutritional solution process, with improve survive Rate, the method that the present invention is adopted is simple, and materials source is wide, and low cost, output of seedling is big, to improve plantation income.

Claims (5)

1. a kind of method for culturing seedlings of Elaeocarpus decipiens, it is characterised in that step includes as follows:
(1) harvesting ripe Elaeocarpus decipiens fruit, is 10 by itself and the clever shell of Semen Tritici aestivi, Baijunling by weight:5:0.02 be mixed after carry out heap Put and macerate rotten 5-7 days, after then being air-dried with water elutriation removal peel and Semen Tritici aestivi grain husk shell placement ventilation, Elaeocarpus decipiens seed and adjuvant are entered Row mixing carries out cellar storage, and it is 33-38% to control the water content of compound, the mixing weight of described Elaeocarpus decipiens seed and adjuvant Amount is than being 1:1, sealing cellar storage grows tender shoots to the Elaeocarpus decipiens seed broken shell for having 40-50% months in next year 2;
(2) in the booth in month in spring 2, nursery is carried out with bag containing soilless substrate, each bag containing soilless substrate is placed the Elaeocarpus decipiens after 1-2 grains store sth. in a cellar and broken Shell seed bud, and coating 3-4mm thick hypothalluses, the substrate in the bag containing soilless substrate by peat, Vermiculitum, plant ash, decomposed manure, 50% sulfur suspending agent, by its weight 13 are compared:14:8:6:0.01 mixing is formed, and its water content 40-45% treats that sprout exposes substrate After pour time permeable, and control temperature of shed, on 22-28 DEG C of daytime, at 18-22 DEG C of night, per time slow water is poured within 5-7 days, wait seedling length extremely High 31-42cm can be transplanted;
(3) in the winter time December to transplanting carries out ploughing deeply 34-40cm, to transplanting every then in month in spring 2, the last ten-days period Mu applies the 33-36cm that turns over after decomposed manure 1800-2000kg, applies N15, P15, K15 to the first mu in transplanting ground before transplanting and answers The 30-33cm that turns over is carried out after Hefei 45-50kg and carbamide 40-50kg, and makees a width of 50-60cm furrow, a height of 30-35cm, furrow ditch Wide 20-25cm, and make the kind plant hole that a diameter of 20-25cm, depth are 10-15cm on furrow;
(4) the Elaeocarpus decipiens seedling in step (2) is first removed after bag containing soilless substrate, band soil libation at an ancient wedding ceremony nutritional solution B is transplanted in step (3) and transplants ground Kind plant hole in banket, then pour permeable, carry out middle weeding, and carry out dry antiperspirant immediately to water, waterlogging caused by excessive rain drainage works, together When carry out at rainy season pest and disease damage prevention daily management.
2. the method for culturing seedlings of a kind of Elaeocarpus decipiens according to claim 1, it is characterised in that the adjuvant described in step (1) is Fine sand, clever shell, herb liquid, nutritional solution A make, and it is 20 that it prepares part by weight:8:0.1:0.1;Its preparation steps is:First by Medicinal liquid be diluted to 200 times of liquid Elaeocarpus decipiens seed is carried out being sprayed to its surface it is micro- it is wet after, mix with fine sand, clever shell and nutritional solution It is even.
3. the method for culturing seedlings of a kind of Elaeocarpus decipiens according to claim 2, it is characterised in that nutritional solution A is by weight being 1:1: 2:2:8 brassin lactones, indolebutyric acid, compound sodium nitrophenolate, diethyl aminoethyl hexanoate and ethanol are mixed.
4. a kind of method for culturing seedlings of Elaeocarpus decipiens according to claim 1, it is characterised in that the kind plant hole described in step (3) Spacing be 40-50cm.
5. a kind of method for culturing seedlings of Elaeocarpus decipiens according to claim 1, it is characterised in that the nutritional solution B described in step (4) By weight it is 0.1 by Bombyx mori L., brassin lactones, compound sodium nitrophenolate, cattle manure:0.2:0.2:5 mixings are formed.
CN201610960005.8A 2016-10-28 2016-10-28 Seedling method for elaeocarpusdecipiens Withdrawn CN106613629A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111066545A (en) * 2020-01-02 2020-04-28 广州普邦园林股份有限公司 Method for preventing Duying transplantation ulcer disease

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CN103250538A (en) * 2013-05-15 2013-08-21 怒江滇鑫农业科技示范园 Seedling culturing method for Chinese yew
CN105085006A (en) * 2015-07-25 2015-11-25 安徽博发文化生态园有限公司 Vegetable seedling-raising nutrient soil substrate and preparation method thereof
CN105110942A (en) * 2015-08-29 2015-12-02 固镇县传红家庭农场 Luffa seedling nutrient substrate

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Publication number Priority date Publication date Assignee Title
CN102084774A (en) * 2010-11-05 2011-06-08 中国热带农业科学院椰子研究所 Method for raising seedling of oil palm seed
CN103250538A (en) * 2013-05-15 2013-08-21 怒江滇鑫农业科技示范园 Seedling culturing method for Chinese yew
CN103250538B (en) * 2013-05-15 2014-07-30 怒江滇鑫农业科技示范园 Seedling culturing method for Chinese yew
CN105085006A (en) * 2015-07-25 2015-11-25 安徽博发文化生态园有限公司 Vegetable seedling-raising nutrient soil substrate and preparation method thereof
CN105110942A (en) * 2015-08-29 2015-12-02 固镇县传红家庭农场 Luffa seedling nutrient substrate

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Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111066545A (en) * 2020-01-02 2020-04-28 广州普邦园林股份有限公司 Method for preventing Duying transplantation ulcer disease
CN111066545B (en) * 2020-01-02 2022-01-14 广州普邦园林股份有限公司 Method for preventing Duying transplantation ulcer disease

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