CN111039713A - Probiotic fertilizer with lactobacillus extracellular polysaccharide and preparation process thereof - Google Patents
Probiotic fertilizer with lactobacillus extracellular polysaccharide and preparation process thereof Download PDFInfo
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- CN111039713A CN111039713A CN201911106374.0A CN201911106374A CN111039713A CN 111039713 A CN111039713 A CN 111039713A CN 201911106374 A CN201911106374 A CN 201911106374A CN 111039713 A CN111039713 A CN 111039713A
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- fertilizer
- acid bacteria
- lactic acid
- potassium
- nitrogen
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a probiotic fertilizer with lactobacillus extracellular polysaccharide, which comprises the following raw material components in percentage by weight: 1-9% of potassium phosphite, 48-58% of nitrogen-phosphorus-potassium compound fertilizer, 40-55% of fermentation active material and 1-10% of resistance additive; the nitrogen-phosphorus-potassium compound fertilizer is prepared from a nitrogen fertilizer, a phosphate fertilizer and a potassium fertilizer according to the weight ratio of 1:1:1 or 2:1: 1; the fermentation active material comprises a biomass material, lactic acid bacteria, glycosyl and water, wherein the weight ratio of the biomass material to the lactic acid bacteria to the glycosyl to the water is 6:1:1: 2; the resistance additive is a mixture of triazolone and polyoxyethylene sorbitol in a weight ratio of 1: 1. The fertilizer provided by the invention can effectively ensure the normal growth of plants, improve the disease resistance of the plants, ferment the active materials to generate extracellular polysaccharide, and adjust an immune system after the plants absorb the extracellular polysaccharide, so that the capacity of resisting the external environment is enhanced, and the survival rate of the plants is improved.
Description
Technical Field
The invention relates to a fertilizer, in particular to a probiotic fertilizer with lactobacillus extracellular polysaccharide and a preparation process thereof.
Background
The fertilizer is a substance which provides one or more nutrient elements necessary for plants, improves the soil property and improves the soil fertility level, and is one of the material bases of agricultural production. Mainly comprises ammonium phosphate fertilizer, macroelement water-soluble fertilizer, secondary element fertilizer, biological fertilizer, organic fertilizer, multi-dimensional field energy concentrated organic fertilizer and the like.
Although various existing fertilizers can provide relatively comprehensive nutrient elements and provide essential components for plant growth, the absorption rate of plants for absorbing fertilizers is not high, most of the existing fertilizers only provide nutrient elements but cannot improve the disease resistance and the immunity of the plants, so that the plants are easy to suffer from pathological changes and death due to fertilization, the survival rate of the plants is reduced, and harvest is influenced.
Disclosure of Invention
The invention aims to solve the defects that the disease resistance and the immunity of plants cannot be improved in the fertilizer in the prior art, and provides a probiotic fertilizer with extracellular polysaccharide of lactic acid bacteria and a preparation process thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
the probiotic fertilizer with the extracellular polysaccharide of the lactic acid bacteria comprises the following raw material components in percentage by weight: 1-9% of potassium phosphite, 48-58% of nitrogen-phosphorus-potassium compound fertilizer, 40-55% of fermentation active material and 1-10% of resistance additive;
the nitrogen-phosphorus-potassium compound fertilizer is prepared from a nitrogen fertilizer, a phosphate fertilizer and a potassium fertilizer according to the weight ratio of 1:1:1 or 2:1: 1;
the fermentation active material comprises a biomass material, lactic acid bacteria, glycosyl and water, wherein the weight ratio of the biomass material to the lactic acid bacteria to the glycosyl to the water is 6:1:1: 2;
the resistance additive is a mixture of triazolone and polyoxyethylene sorbitol in a weight ratio of 1: 1.
Preferably, the probiotic fertilizer with the extracellular polysaccharide of the lactic acid bacteria comprises the following raw material components in percentage by weight: 1-7% of potassium phosphite, 50-56% of nitrogen-phosphorus-potassium compound fertilizer, 42-53% of fermentation active material and 1-8% of resistance additive.
Preferably, the probiotic fertilizer with the extracellular polysaccharide of the lactic acid bacteria comprises the following raw material components in percentage by weight: 2-5% of potassium phosphite, 51-54% of nitrogen-phosphorus-potassium compound fertilizer, 43-53% of fermentation active material and 3-6% of resistance additive.
Preferably, the nitrogen fertilizer is ammonium bicarbonate, the phosphate fertilizer is calcium phosphate, and the potassium fertilizer is potassium chloride.
Preferably, the biomass material is crop stalks.
Preferably, the biomass material is one or two mixtures or three mixtures of straws, rice straws or corn stalks.
A preparation process of a probiotic fertilizer with extracellular polysaccharide of lactic acid bacteria comprises the following steps:
s1, weighing the potassium phosphite, the nitrogen-phosphorus-potassium compound fertilizer, the fermentation active material and the resistance additive in percentage by weight for later use;
s2, crushing the weighed biomass materials in the fermentation active materials by a crusher, and mixing the prepared material particles, lactic acid bacteria, glycosyl and water according to the weight ratio of 6:1:1: 2;
s3, fermenting the mixture prepared in the S2 at the temperature of 37-42 ℃ for 8-12 hours to generate acid bacteria extracellular polysaccharide and prepare an intermediate product;
and S4, adding the potassium phosphite, the nitrogen-phosphorus-potassium compound fertilizer and the resistance additive weighed in the step S1 into the intermediate product, and uniformly stirring for 2 hours at a speed of 40-60r/min by using a stirrer to obtain a colloidal substance.
And S5, drying the colloidal substance generated in the S4 at a constant temperature of 60-80 ℃ to obtain the solid fertilizer.
Preferably, the solid fertilizer is made into granular or powdery fertilizer dissolved in water by a granulator or a pulverizer.
Compared with the prior art, the invention has the beneficial effects that:
the probiotic fertilizer with the extracellular polysaccharide of the lactic acid bacteria, provided by the invention, provides nutrient elements necessary for plant growth, so that normal growth of plants is ensured, the disease resistance of the plants can be improved by the potassium phosphite and the resistance additive, the extracellular polysaccharide can be generated by the fermentation active material, and the extracellular polysaccharide generated by the lactic acid bacteria is stable, safe, nontoxic and free of side effects, so that the immune system can be adjusted after the plants absorb the extracellular polysaccharide, the capacity of resisting the external environment is enhanced, and the survival rate of the plants is improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
The probiotic fertilizer with the extracellular polysaccharide of the lactic acid bacteria comprises the following raw material components in percentage by weight: 1-9% of potassium phosphite, 48-58% of nitrogen-phosphorus-potassium compound fertilizer, 40-55% of fermentation active material and 1-10% of resistance additive;
the nitrogen-phosphorus-potassium compound fertilizer is prepared from a nitrogen fertilizer, a phosphate fertilizer and a potassium fertilizer according to the weight ratio of 1:1:1 or 2:1: 1;
the fermentation active material comprises a biomass material, lactic acid bacteria, glycosyl and water, wherein the weight ratio of the biomass material to the lactic acid bacteria to the glycosyl is 6:1:1: 2;
the resistance additive is a mixture of triazolone and polyoxyethylene sorbitol in a weight ratio of 1: 1.
The probiotic fertilizer with the lactobacillus extracellular polysaccharide comprises the following raw material components in percentage by weight: 1-7% of potassium phosphite, 50-56% of nitrogen-phosphorus-potassium compound fertilizer, 42-53% of fermentation active material and 1-8% of resistance additive.
The probiotic fertilizer with the lactobacillus extracellular polysaccharide comprises the following raw material components in percentage by weight: 2-5% of potassium phosphite, 51-54% of nitrogen-phosphorus-potassium compound fertilizer, 43-53% of fermentation active material and 3-6% of resistance additive.
The nitrogenous fertilizer is ammonium bicarbonate, the phosphate fertilizer is calcium phosphate, and the potash fertilizer is potassium chloride.
The biomass material is crop stalks.
The biomass material is one or two or three of straw, rice straw or corn stalk.
A preparation process of a probiotic fertilizer with extracellular polysaccharide of lactic acid bacteria comprises the following steps:
s1, weighing the potassium phosphite, the nitrogen-phosphorus-potassium compound fertilizer, the fermentation active material and the resistance additive in percentage by weight for later use;
s2, crushing the weighed biomass materials in the fermentation active materials by a crusher, and mixing the prepared material particles, lactic acid bacteria, glycosyl and water according to the weight ratio of 6:1:1: 2;
s3, fermenting the mixture prepared in the S2 at the temperature of 37-42 ℃ for 8-12 hours to generate acid bacteria extracellular polysaccharide and prepare an intermediate product;
and S4, adding the potassium phosphite, the nitrogen-phosphorus-potassium compound fertilizer and the resistance additive weighed in the step S1 into the intermediate product, and uniformly stirring for 2 hours at a speed of 40-60r/min by using a stirrer to obtain a colloidal substance.
And S5, drying the colloidal substance generated in the S4 at a constant temperature of 60-80 ℃ to obtain the solid fertilizer.
The solid fertilizer is made into granular or powdery fertilizer dissolved in water by a granulator or a pulverizer.
Example one
The probiotic fertilizer with the extracellular polysaccharide of the lactic acid bacteria comprises the following raw material components in percentage by weight: 1% of potassium phosphite, 55% of nitrogen-phosphorus-potassium compound fertilizer, 43% of fermentation active material and 1% of resistance additive.
Example two
The probiotic fertilizer with the extracellular polysaccharide of the lactic acid bacteria comprises the following raw material components in percentage by weight: 3% of potassium phosphite, 48% of nitrogen-phosphorus-potassium compound fertilizer, 45% of fermentation active material and 4% of resistance additive.
EXAMPLE III
The probiotic fertilizer with the extracellular polysaccharide of the lactic acid bacteria comprises the following raw material components in percentage by weight: 1% of potassium phosphite, 48% of nitrogen-phosphorus-potassium compound fertilizer, 50% of fermentation active material and 1% of resistance additive.
A control experiment is set to verify the synergistic effect of the probiotic fertilizer on the growth of plants.
Subject: leaf of Chinese lettuce.
Experiment design: four farmlands with similar soil quality are selected for planting leaf lettuce, the area of each farmland is 1 mu, fertilizers are prepared according to the weight percentage of the components in the first embodiment, the second embodiment and the third embodiment, and only nitrogen-phosphorus-potassium compound fertilizers are applied to the control group.
And (3) recording an experiment: observing the growth vigor of the leaf lettuce, and recording the number of surviving and dead plants, plant height, leaf number, single plant weight and yield per mu.
Taoyi lettuce survival rate statistical table
| Example one | Example two | EXAMPLE III | Control group | |
| Number of surviving plants | 1831 | 1922 | 1940 | 1832 |
| Number of dead plants | 21 | 19 | 15 | 95 |
| Survival rate | 98.87％ | 99.02％ | 99.23％ | 95.07％ |
Sedum indicum and leaf lettuce growth statistical table
| Example one | Example two | EXAMPLE III | Control group | |
| Investigating strain number | 40 | 40 | 40 | 40 |
| Plant height (cm) | 13.2 | 13.4 | 13.5 | 12.6 |
| Number of blades (sheet) | 8.5 | 8.7 | 8.6 | 7.8 |
| Weight of single plant | 34.3 | 36.2 | 36.5 | 30.1 |
Mu yield statistical table for Otsumadai leaf lettuce
| Example one | Example two | EXAMPLE III | Control group | |
| Mu yield (kg) | 2239.3 | 2364.8 | 2379.2 | 1903.4 |
And (5) drawing a conclusion that: as can be seen from the above tables 1-3, the probiotic fertilizer of the present invention can effectively ensure the normal growth of plants, improve the disease resistance of plants, ferment the active materials to produce extracellular polysaccharide, and the extracellular polysaccharide produced by lactic acid bacteria is stable, safe, non-toxic, and free from side effects, and after the extracellular polysaccharide is absorbed by plants, the plants can adjust the immune system, so as to enhance the ability of the plants to resist the external environment, and improve the survival rate of the plants.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. The probiotic fertilizer with the extracellular polysaccharide of the lactic acid bacteria is characterized by comprising the following raw material components in percentage by weight: 1-9% of potassium phosphite, 48-58% of nitrogen-phosphorus-potassium compound fertilizer, 40-55% of fermentation active material and 1-10% of resistance additive;
the nitrogen-phosphorus-potassium compound fertilizer is prepared from a nitrogen fertilizer, a phosphate fertilizer and a potassium fertilizer according to the weight ratio of 1:1:1 or 2:1: 1;
the fermentation active material comprises a biomass material, lactic acid bacteria, glycosyl and water, wherein the weight ratio of the biomass material to the lactic acid bacteria to the glycosyl to the water is 6:1:1: 2;
the resistance additive is a mixture of triazolone and polyoxyethylene sorbitol in a weight ratio of 1: 1.
2. The probiotic fertilizer with extracellular polysaccharide of lactic acid bacteria according to claim 1, characterized by comprising the following raw material components in percentage by weight: 1-7% of potassium phosphite, 50-56% of nitrogen-phosphorus-potassium compound fertilizer, 42-53% of fermentation active material and 1-8% of resistance additive.
3. The probiotic fertilizer with extracellular polysaccharide of lactic acid bacteria according to claim 1, characterized by comprising the following raw material components in percentage by weight: 2-5% of potassium phosphite, 51-54% of nitrogen-phosphorus-potassium compound fertilizer, 43-53% of fermentation active material and 3-6% of resistance additive.
4. The probiotic fertilizer with extracellular polysaccharides of lactic acid bacteria according to any one of claims 1 to 3, characterized in that the nitrogen fertilizer is ammonium bicarbonate, the phosphate fertilizer is calcium phosphate, and the potassium fertilizer is potassium chloride.
5. The probiotic fertilizer with extracellular polysaccharides of lactic acid bacteria according to any one of claims 1 to 3, characterized in that the biomass material is crop stalks.
6. The probiotic fertilizer with extracellular polysaccharides of lactic acid bacteria according to claim 5, characterized in that the biomass material is one or a mixture of two or a mixture of three of straw, rice straw or corn stalks.
7. A preparation process of a probiotic fertilizer with extracellular polysaccharide of lactic acid bacteria is characterized by comprising the following steps:
s1, weighing the potassium phosphite, the nitrogen-phosphorus-potassium compound fertilizer, the fermentation active material and the resistance additive in percentage by weight for later use;
s2, crushing the weighed biomass materials in the fermentation active materials by a crusher, and mixing the prepared material particles, lactic acid bacteria, glycosyl and water according to the weight ratio of 6:1:1: 2;
s3, fermenting the mixture prepared in the S2 at the temperature of 37-42 ℃ for 8-12 hours to generate acid bacteria extracellular polysaccharide and prepare an intermediate product;
and S4, adding the potassium phosphite, the nitrogen-phosphorus-potassium compound fertilizer and the resistance additive weighed in the step S1 into the intermediate product, and uniformly stirring for 2 hours at a speed of 40-60r/min by using a stirrer to obtain a colloidal substance.
And S5, drying the colloidal substance generated in the S4 at a constant temperature of 60-80 ℃ to obtain the solid fertilizer.
8. The process for preparing a probiotic fertilizer with extracellular polysaccharides of lactic acid bacteria according to claim 7, wherein the solid fertilizer is made into granular or powdery fertilizer dissolved in water by a granulator or a pulverizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911106374.0A CN111039713A (en) | 2019-11-13 | 2019-11-13 | Probiotic fertilizer with lactobacillus extracellular polysaccharide and preparation process thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911106374.0A CN111039713A (en) | 2019-11-13 | 2019-11-13 | Probiotic fertilizer with lactobacillus extracellular polysaccharide and preparation process thereof |
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| CN111039713A true CN111039713A (en) | 2020-04-21 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935750A (en) * | 2005-09-20 | 2007-03-28 | 刘家宝 | Anti-plant-virus fertilizer additivesand fertilizer containing them |
| CN103130553A (en) * | 2011-11-21 | 2013-06-05 | 南通中科睿智科技服务有限公司 | Antibiotic biologically-active selenium-enriched organic fertilizer |
| CN108707049A (en) * | 2018-08-03 | 2018-10-26 | 甘肃米香原农业科技有限责任公司 | A kind of watermelon lactic acid bacteria bacterial manure and preparation method thereof |
| CN109824446A (en) * | 2019-04-16 | 2019-05-31 | 漳州三炬生物技术有限公司 | A kind of Water soluble fertilizer and its preparation method and application with controlling disease function |
-
2019
- 2019-11-13 CN CN201911106374.0A patent/CN111039713A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935750A (en) * | 2005-09-20 | 2007-03-28 | 刘家宝 | Anti-plant-virus fertilizer additivesand fertilizer containing them |
| CN103130553A (en) * | 2011-11-21 | 2013-06-05 | 南通中科睿智科技服务有限公司 | Antibiotic biologically-active selenium-enriched organic fertilizer |
| CN108707049A (en) * | 2018-08-03 | 2018-10-26 | 甘肃米香原农业科技有限责任公司 | A kind of watermelon lactic acid bacteria bacterial manure and preparation method thereof |
| CN109824446A (en) * | 2019-04-16 | 2019-05-31 | 漳州三炬生物技术有限公司 | A kind of Water soluble fertilizer and its preparation method and application with controlling disease function |
Non-Patent Citations (1)
| Title |
|---|
| 罗玲泉: "酸乳胞外多糖提取及高产胞外多糖发酵剂研究", 《湖南农业大学》 * |
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Application publication date: 20200421 |