CN110974770A - Anti-aging composition, preparation and preparation method - Google Patents
Anti-aging composition, preparation and preparation method Download PDFInfo
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
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- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/925—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of animal origin
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
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- A61Q19/08—Anti-ageing preparations
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- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The invention provides an anti-aging composition, a preparation and a preparation method, belonging to the technical field of daily skin care, wherein the anti-aging composition comprises the following raw materials in percentage by mass: 1-99 wt% of animal and plant cell extract containing cell factor; 0.01-40 wt% of snake oil; 0.01-1 wt% of cordyceps extract, the anti-aging composition provided by the invention adopts the animal and plant cell extract containing cell factors, snake oil and the cordyceps extract to be matched with each other to play a synergistic effect, not only can the protection effect on cells damaged by oxidation be obviously improved, but also the water content of skin can be improved, the wrinkle area can be reduced, the melanin distribution can be reduced, the anti-aging effect is obvious, and the composition is simple, the toxic and side effects are small, and the safety is high.
Description
Technical Field
The invention relates to the technical field of daily skin care, and particularly relates to an anti-aging composition, a preparation and a preparation method.
Background
Skin aging easily causes a series of problems such as wrinkles, sagging, dryness, and pigment deposition. Skin aging includes two aging processes, namely natural aging which is determined by intrinsic genes; the second is aging caused by external environmental factors, such as ultraviolet rays, pollutants, and the like. The mechanism of skin aging is very complex. The prior anti-aging cosmetics use industrial components with strong oxidation resistance to achieve good anti-aging effect, take effect quickly in a short time, but have great harm to human bodies after being used for a long time.
The stem cells can activate the self-healing function of human bodies, supplement and regulate diseased cells, activate the cell function, increase the number of normal cells, improve the activity of the cells, improve the quality of the cells, prevent and delay the pathological changes of the cells and restore the normal physiological function of the cells, thereby achieving the aim of disease rehabilitation.
Patent CN110025566A filter-sterilizes the supernatant obtained by culturing adipose-derived stem cells to obtain a cosmetic based on the stem cell culture supernatant. The number of passages of the adipose-derived stem cells is limited, and the adipose-derived stem cells are required to be separated from adipose tissues frequently. In patent CN110129392A, the collected umbilical cord mesenchymal stem cells are added with deionized water for lysis, then the cell debris is removed by low-temperature centrifugation, and the supernatant is freeze-dried to obtain the umbilical cord mesenchymal stem cell factor. However, the research finds that when the single stem cell culture solution is applied to the skin anti-aging research, the defects of poor release performance, low skin storage amount and low efficacy exist, so that the effect time is long, and a good anti-aging function cannot be obtained.
Disclosure of Invention
Therefore, the invention provides an anti-aging composition, a preparation and a preparation method, aiming at overcoming the defects of poor anti-aging effect and low safety of stem cell culture solution in the prior art.
The invention provides an anti-aging composition which comprises the following raw materials in percentage by mass:
1-99 wt% of animal and plant cell extract containing cell factor;
0.01-40 wt% of snake oil;
0.01-1 wt% of cordyceps extract.
Further, the composite material comprises the following raw materials in percentage by mass:
30-70 wt% of animal and plant cell extract containing cell factor;
5-25 wt% of snake oil;
0.1-0.5 wt% of cordyceps extract.
Further, the animal and plant cell extract containing the cell factors is at least one of an embryonic stem cell culture solution, an animal umbilical cord extract, a plant cell extract and a placenta extract.
Further, the plant cell extract is a plant stem cell extract, which may be, but is not limited to, apple stem cell extract.
Further, the preparation method of the embryonic stem cell culture solution comprises the following steps:
transferring the embryonic stem cells into a serum-free culture medium for subculture, periodically replacing a culture solution, collecting the replaced culture solution, filtering, desalting and concentrating to obtain the embryonic stem cell culture solution.
The invention provides an anti-aging preparation, which comprises any one of the anti-aging compositions.
Further, the anti-aging preparation is toner, lotion, eye cream, gel, cream, essence or facial mask.
Further, the anti-aging preparation comprises the following components in percentage by mass:
0.1-5 wt% of emulsifier;
24-42 wt% of water;
the anti-aging composition accounts for 35 to 80 weight percent.
Further, the anti-aging preparation further comprises the following components in percentage by mass:
35-80 wt% of any of the anti-aging compositions described above.
Further, the humectant is at least one of glycerol, propylene glycol, butanediol, hyaluronic acid, panthenol, trehalose and dextran;
the emulsifier is at least one of PEG-100 stearate, potassium cetyl phosphate, glyceryl stearate and span 60;
the thickening agent is at least one of xanthan gum, carbomer and hydroxyethyl cellulose;
the emollient is at least one of GTCC, cetearyl alcohol, isopropyl palmitate, lanolin and dimethicone;
the preservative is at least one of phenoxyethanol, methyl paraben, propyl paraben, chlorphenesin and bis (hydroxymethyl) imidazolidinyl urea;
the antioxidant is at least one of tocopherol and vitamin C, BHT.
Further, the humectant is prepared from the following components in a mass ratio of (0.1-0.5): (1-10): (1-5) hyaluronic acid, glycerol, butylene glycol;
the emollient is prepared from the following components in percentage by mass (1-6): (1-8) isopropyl palmitate and dimethicone.
The invention provides a method for preparing any one of the above preparations, comprising the following steps:
preparing an aqueous phase solution: adding the humectant and the cordyceps extract into water, dissolving and mixing uniformly, adding the emulsifier and the thickener, homogenizing until the mixture is uniformly dispersed, then adding the embryonic stem cell culture solution and the preservative, and stirring uniformly to obtain a water-phase solution;
preparing an oil phase solution: uniformly mixing an emollient, snake oil and an antioxidant to obtain an oil phase solution for later use;
an emulsification step: mixing the water phase solution and the oil phase solution, and homogenizing to obtain the final product.
Further, in the aqueous phase solution preparation step and/or the emulsification step, the homogenization speed is 2000-5000rpm, the stirring speed is 10-50rpm, and the time is 3-10min during the homogenization process.
The technical scheme of the invention has the following advantages:
(1) the anti-aging composition provided by the invention adopts the animal and plant cell extract containing the cell factors, the snake oil and the cordyceps extract to be matched with each other, and the synergistic effect is exerted, so that the protective effect on cells damaged by oxidation can be obviously improved, the water content of skin can be improved, the wrinkle area can be reduced, the melanin distribution can be reduced, the anti-aging effect is obviously improved, and the composition is simple, the toxic and side effects are small, and the safety is high.
(2) The anti-aging composition provided by the invention adopts a serum-free culture medium to culture the embryonic stem cells to obtain a serum-free embryonic stem cell culture solution, so that the safety of the composition is further improved.
(3) The anti-aging preparation provided by the invention is prepared by preferably selecting the following components in a mass ratio of (0.1-0.5): (1-10): (1-5) taking hyaluronic acid, glycerol and butanediol as moisturizers, wherein the mass ratio of the hyaluronic acid to the glycerol to the butanediol is (1-6): the isopropyl palmitate and the simethicone in the formula (1-8) are used as the emollients, and the obtained essence has proper viscosity, strong moisturizing force and good use feeling, and is easier to promote skin absorption, so that a better anti-aging effect is obtained.
(4) According to the preparation method of the anti-aging preparation, the cordyceps sinensis extract, the embryonic stem cell culture solution, the humectant, the thickener, the emulsifier and the preservative are mixed to form the water phase solution, the emollient, the snake oil and the antioxidant are mixed to obtain the oil phase solution, and then the water phase solution and the oil phase solution are homogenized, so that the oil phase and the water phase of the essence both contain anti-aging ingredients; in addition, in the homogenizing process, the oil phase containing the anti-aging ingredients in the essence can be uniformly dispersed in the water phase in a micro-bead form to form the essence with uniform dispersion and good stability.
Detailed Description
Example 1
The embodiment provides an anti-aging composition and a preparation method thereof, and the raw materials comprise the following components:
41g of embryonic stem cell culture solution, 15g of snake oil, 0.5g of cordyceps extract, 3g of potassium cetyl phosphate and 40.5g of water.
Preparation of embryonic Stem cell culture solution
Transferring the embryonic stem cells into a culture bottle containing a serum-free culture medium for subculture for 2 days, replacing the culture solution every 2 days, collecting the culture solution replaced every time, filtering the culture solution through a filter membrane with the aperture of 0.22 micrometer, desalting and concentrating to 1/10 of the original volume to obtain the embryonic stem cell culture solution.
Preparation of anti-aging composition
Weighing the embryonic stem cell culture solution, snake oil, the cordyceps extract, potassium cetyl phosphate and water according to the formula, dissolving the cordyceps extract and the potassium cetyl phosphate in the water, adding the embryonic stem cell culture solution and the snake oil, and homogenizing at a homogenization speed of 3000rpm and a stirring speed of 30rpm for 5min to obtain the compound.
Example 2
The embodiment provides an anti-aging composition and a preparation method thereof, and raw materials of the composition comprise the following components:
30g of embryonic stem cell culture solution, 25g of snake oil, 0.2g of cordyceps extract, 5g of potassium cetyl phosphate and 39.8g of water.
Preparation of embryonic Stem cell culture solution
Transferring the embryonic stem cells into a culture bottle containing a serum-free culture medium for culturing for 2 days, replacing the culture solution every 2 days, collecting the culture solution replaced every time, filtering the culture solution through a filter membrane with the aperture of 0.22 micrometer, desalting and concentrating to 1/10 of the original volume to obtain the embryonic stem cell culture solution.
Preparation of anti-aging composition
Weighing the embryonic stem cell culture solution, snake oil, the cordyceps extract, potassium cetyl phosphate and water according to the formula, dissolving the cordyceps extract and the potassium cetyl phosphate in the water, adding the embryonic stem cell culture solution and the snake oil, and homogenizing at a homogenization speed of 3000rpm and a stirring speed of 30rpm for 5min to obtain the compound.
Example 3
The embodiment provides an anti-aging composition and a preparation method thereof, and raw materials of the composition comprise the following components:
70g of embryonic stem cell culture solution, 5g of snake oil, 0.3g of cordyceps extract, 0.1g of potassium cetyl phosphate and 24.6g of water.
Preparation of embryonic Stem cell culture solution
Transferring the embryonic stem cells into a culture bottle containing a serum-free culture medium for culturing for 2 days, replacing the culture solution every 2 days, collecting the culture solution replaced every time, filtering the culture solution through a filter membrane with the aperture of 0.22 micrometer, desalting and concentrating to 1/10 of the original volume to obtain the embryonic stem cell culture solution.
Preparation of anti-aging composition
Weighing the embryonic stem cell culture solution, snake oil, the cordyceps extract, potassium cetyl phosphate and water according to the formula, dissolving the cordyceps extract and the potassium cetyl phosphate in the water, adding the embryonic stem cell culture solution and the snake oil, and homogenizing at a homogenization speed of 3000rpm and a stirring speed of 30rpm for 5min to obtain the compound.
Example 4
The embodiment provides anti-aging essence and a preparation method thereof, and the anti-aging essence comprises the following raw materials:
41g of embryonic stem cell culture solution, 15g of snake oil, 0.5g of cordyceps extract, 0.2g of hyaluronic acid, 1.8g of butanediol, 2g of glycerol, 3g of potassium cetyl phosphate, 0.2g of carbomer, 3g of isopropyl palmitate, 4g of simethicone, 0.5g of phenoxyethanol, 0.2g of methylparaben, 0.5g of tocopherol and 28.4g of water.
Preparation of embryonic Stem cell culture solution
Transferring the embryonic stem cells into a culture bottle containing a serum-free culture medium for culturing for 2 days, replacing the culture solution every 2 days, collecting the culture solution replaced every time, filtering the culture solution through a filter membrane with the aperture of 0.22 micrometer, desalting and concentrating to 1/10 of the original volume to obtain the embryonic stem cell culture solution.
Preparation of anti-aging essence
(1) Preparing an aqueous phase solution: mixing hyaluronic acid and glycerol, dispersing until no agglomeration, adding into water, adding Cordyceps extract, stirring to dissolve, and mixing; adding cetyl potassium phosphate and carbomer, homogenizing at 3000rpm and 30rpm stirring speed for 5min, adding mixture of embryonic stem cell culture solution, phenoxyethanol and methyl paraben, and stirring to obtain water phase solution;
(2) preparing an oil phase solution: mixing isopropyl palmitate, simethicone, tocopherol and snake oil, and stirring at the rotating speed of 30rpm until the isopropyl palmitate, the simethicone, the tocopherol and the snake oil are uniformly mixed to obtain an oil phase solution;
(3) an emulsification step: mixing the water phase solution and the oil phase solution, homogenizing at 3000rpm and 30rpm for 5min, adding triethanolamine, and adjusting pH to 7.0-7.5.
Example 5
The embodiment provides anti-aging essence and a preparation method thereof, and the anti-aging essence comprises the following raw materials:
41g of embryonic stem cell culture solution, 15g of snake oil, 0.5g of cordyceps extract, 4g of glycerol, 3g of potassium cetyl phosphate, 0.2g of carbomer, 3g of isopropyl palmitate, 4g of simethicone, 0.5g of phenoxyethanol, 0.2g of methylparaben, 0.5g of tocopherol and 28.4g of water.
Preparation of embryonic Stem cell culture solution
Transferring the embryonic stem cells into a culture bottle containing a serum-free culture medium for culturing for 2 days, replacing the culture solution every 2 days, collecting the culture solution replaced every time, filtering the culture solution through a filter membrane with the aperture of 0.22 micrometer, desalting and concentrating to 1/10 of the original volume to obtain the embryonic stem cell culture solution.
Preparation of anti-aging essence
(1) Preparing an aqueous phase solution: adding Cordyceps extract into water, stirring to dissolve, adding glycerol, potassium cetyl phosphate, and carbomer, homogenizing at 3000rpm and 30rpm for 5min, adding embryonic stem cell culture solution, phenoxyethanol and methyl hydroxybenzoate mixture, and stirring to obtain water phase solution;
(2) preparing an oil phase solution: mixing isopropyl palmitate, simethicone, tocopherol and snake oil, and stirring at the rotating speed of 30rpm until the isopropyl palmitate, the simethicone, the tocopherol and the snake oil are uniformly mixed to obtain an oil phase solution;
(3) an emulsification step: mixing the water phase solution and the oil phase solution, homogenizing at 3000rpm and 30rpm for 5min, adding triethanolamine, and adjusting pH to 7.0-7.5.
Example 6
The embodiment provides anti-aging essence and a preparation method thereof, and the anti-aging essence comprises the following raw materials:
41g of embryonic stem cell culture solution, 15g of snake oil, 0.5g of cordyceps extract, 0.2g of hyaluronic acid, 1.8g of butanediol, 2g of glycerol, 3g of potassium cetyl phosphate, 0.2g of carbomer, 3g of GTCC, 4g of simethicone, 0.5g of phenoxyethanol, 0.2g of methylparaben, 0.5g of tocopherol and 28.4g of water.
Preparation of embryonic Stem cell culture solution
Transferring the embryonic stem cells into a culture bottle containing a serum-free culture medium for culturing for 2 days, replacing the culture solution every 2 days, collecting the culture solution replaced every time, filtering the culture solution through a filter membrane with the aperture of 0.22 micrometer, desalting and concentrating to 1/10 of the original volume to obtain the embryonic stem cell culture solution.
Preparation of anti-aging essence
(1) Preparing an aqueous phase solution: mixing hyaluronic acid and glycerol, dispersing until no agglomeration, adding into water, adding Cordyceps extract, stirring to dissolve, and mixing; adding cetyl potassium phosphate and carbomer, homogenizing at 3000rpm and 30rpm stirring speed for 5min, adding mixture of embryonic stem cell culture solution, phenoxyethanol and methyl paraben, and stirring to obtain water phase solution;
(2) preparing an oil phase solution: mixing GTCC, simethicone, tocopherol and snake oil, and stirring at the rotating speed of 30rpm until the mixture is uniformly mixed to obtain an oil phase solution;
(3) an emulsification step: mixing the water phase solution and the oil phase solution, homogenizing at 3000rpm and 30rpm for 5min, adding triethanolamine, and adjusting pH to 7.0-7.5.
Example 7
The embodiment provides an anti-aging composition and a preparation method thereof, and the raw materials comprise the following components:
41g of umbilical cord extract, 15g of snake oil, 0.5g of cordyceps extract, 3g of potassium cetyl phosphate and 40.5g of water.
Preparation of anti-aging composition
Weighing the bovine umbilical cord extract, snake oil, the cordyceps extract, potassium cetyl phosphate and water according to the formula, dissolving the cordyceps extract and the potassium cetyl phosphate in the water, adding the bovine umbilical cord extract and the snake oil, and homogenizing at a homogenization speed of 3000rpm and a stirring speed of 30rpm for 5min to obtain the feed additive.
Example 8
The embodiment provides an anti-aging composition and a preparation method thereof, and the raw materials comprise the following components:
41g of cow placenta extract, 15g of snake oil, 0.5g of cordyceps extract, 3g of potassium cetyl phosphate and 40.5g of water.
Preparation of anti-aging composition
Weighing cattle placenta extract, snake oil, Cordyceps extract, potassium cetyl phosphate and water according to the prescription amount, dissolving the Cordyceps extract and potassium cetyl phosphate in water, adding the cattle placenta extract and snake oil, and homogenizing at a homogenizing speed of 3000rpm and a stirring speed of 30rpm for 5min to obtain the product.
Example 9
The embodiment provides an anti-aging composition and a preparation method thereof, and the raw materials comprise the following components:
41g of apple stem cell extract, 15g of snake oil, 0.5g of cordyceps extract, 3g of potassium cetyl phosphate and 40.5g of water.
Preparation of anti-aging composition
Weighing apple stem cell extract, snake oil, cordyceps extract, potassium cetyl phosphate and water according to the prescription amount, dissolving the cordyceps extract and the potassium cetyl phosphate in the water, adding the apple stem cell extract and the snake oil, and homogenizing at a homogenizing speed of 3000rpm and a stirring speed of 30rpm for 5min to obtain the apple stem cell extract.
Comparative example 1
The comparative example provides an anti-aging composition and a preparation method thereof, and the raw materials comprise the following components:
41g of embryonic stem cell culture solution, 0.5g of cordyceps extract, 3g of potassium cetyl phosphate and 55.5g of water.
Preparation of embryonic Stem cell culture solution
Transferring the embryonic stem cells into a culture bottle containing a serum-free culture medium for culturing for 2 days, replacing the culture solution every 2 days, collecting the culture solution replaced every time, filtering the culture solution through a filter membrane with the aperture of 0.22 micrometer, desalting and concentrating to 1/10 of the original volume to obtain the embryonic stem cell culture solution.
Preparation of anti-aging composition
Weighing the embryonic stem cell culture solution, the cordyceps extract, the potassium cetyl phosphate and water according to the prescription amount, dissolving the cordyceps extract and the potassium cetyl phosphate in the water, adding the embryonic stem cell culture solution, and uniformly stirring and mixing to obtain the cordyceps extract-potassium cetyl phosphate.
Comparative example 2
The comparative example provides an anti-aging composition and a preparation method thereof, and the raw materials comprise the following components:
41g of embryonic stem cell culture solution, 15g of snake oil, 3g of potassium cetyl phosphate and 41g of water.
Preparation of embryonic Stem cell culture solution
Transferring the embryonic stem cells into a culture bottle containing a serum-free culture medium for culturing for 2 days, replacing the culture solution every 2 days, collecting the culture solution replaced every time, filtering the culture solution through a filter membrane with the aperture of 0.22 micrometer, desalting and concentrating to 1/10 of the original volume to obtain the embryonic stem cell culture solution.
Preparation of anti-aging composition
Weighing the embryonic stem cell culture solution, snake oil, potassium cetyl phosphate and water according to the prescription amount, dissolving the cordyceps extract and the potassium cetyl phosphate in the water, adding the embryonic stem cell culture solution and the snake oil, and homogenizing at a homogenization speed of 3000rpm and a stirring speed of 30rpm for 5min to obtain the cordyceps sinensis extractive solution.
Comparative example 3
The comparative example provides an anti-aging composition and a preparation method thereof, and the raw materials comprise the following components:
41g of embryonic stem cell culture solution, 15g of snake oil, 0.5g of rosemary, 3g of potassium cetyl phosphate and 40.5g of water.
Preparation of embryonic Stem cell culture solution
Transferring the embryonic stem cells into a culture bottle containing a serum-free culture medium for culturing for 2 days, replacing the culture solution every 2 days, collecting the culture solution replaced every time, filtering the culture solution through a filter membrane with the aperture of 0.22 micrometer, desalting and concentrating to 1/10 of the original volume to obtain the embryonic stem cell culture solution.
Preparation of anti-aging composition
Weighing the embryonic stem cell culture solution, the snake oil, the rosemary, the potassium cetyl phosphate and water according to the prescription amount, dissolving the rosemary and the potassium cetyl phosphate in the water, adding the embryonic stem cell culture solution and the snake oil, and homogenizing at a homogenization speed of 3000rpm and a stirring speed of 30rpm for 5min to obtain the compound.
Comparative example 4
The comparative example provides an anti-aging composition and a preparation method thereof, and the raw materials comprise the following components:
41g of umbilical cord extract, 15g of vaseline, 0.5g of cordyceps extract, 3g of potassium cetyl phosphate and 40.5g of water.
Preparation of anti-aging composition
Weighing the bovine umbilical cord extract, vaseline, cordyceps extract, potassium cetyl phosphate and water according to the prescription amount, dissolving the cordyceps extract and the potassium cetyl phosphate in the water, adding the bovine umbilical cord extract and the vaseline, and homogenizing at a homogenization speed of 3000rpm and a stirring speed of 30rpm for 5min to obtain the composition.
Experimental example 1
1. Test method
Preparation of 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) solution: completely dissolving DPPH with absolute ethyl alcohol, preparing a mother solution with the concentration of 0.1mmol/L, storing the mother solution at 4 ℃ in a dark place, and diluting the mother solution to 100 mu mol/L DPPH solution before use.
Preparing a sample solution: the antiaging compositions obtained in examples 1 to 3, examples 7 to 9 and comparative examples 1 to 4 were each formulated with deionized water into sample solutions having a composition concentration of 0.1 mg/ml.
The test group comprises that 100 mu l of each sample solution and 100 mu mol/l of DPPH ethanol solution are respectively taken to be put into a 96-well plate, a blank control group comprises that 100 mu l of each sample solution and 100 mu mol/l of absolute ethanol are respectively taken to be put into a 96-well plate, a standard group comprises that 100 mu l of deionized water and 100 mu mol/l of DPPH ethanol solution are respectively taken to be put into a 96-well plate, the deionized water and the 100 mu mol/l of DPPH ethanol solution are respectively mixed uniformly, after the mixture is kept still for 30min at room temperature, α -arbutin is taken as a positive control, the absorbance of a sample is rapidly measured at the wavelength of 517nm, and the inhibition rate is DPPH clearance rate (%) [1- (sample solution absorbance-blank control group absorbance)/standard group absorbance ] × 100%, wherein when each group is tested, the sample solution of the test group is consistent with the.
TABLE 1 result of DPPH radical scavenging
Item | DPPH clearance (%) |
Example 1 | 89.88 |
Example 2 | 81.73 |
Example 3 | 93.64 |
Example 7 | 83.45 |
Example 8 | 82.92 |
Example 9 | 84.02 |
Comparative example 1 | 62.57 |
Comparative example 2 | 69.29 |
Comparative example 3 | 73.04 |
Comparative example 4 | 59.37 |
As shown in the table, the DPPH free radical clearance rate of the composition is 81% -93%, while the DPPH free radical clearance rate of the comparative examples 1-4 is lower than 75%, which shows that the composition prepared by the invention can obviously improve the DPPH free radical clearance rate and improve the anti-aging function.
Experimental example 2
1. Test method
The antiaging compositions obtained in example 1, example 7 and comparative examples 1 to 4 were stored at room temperature for 1 month, and the contents of EGF, bFGF and TGF- β before and after storage in each composition were measured, respectively, and the content decrease rates of the three active ingredients were calculated, and the results are shown in the following tables.
The EGF content is detected according to the operation method of the EGF ELISA kit, the bFGF content is detected according to the operation method of the bFGF ELISA kit, and the TGF- β content is detected according to the operation method of the TGF- β ELISA kit.
Table 2 active ingredient content results table
Item | EGF decrease (%) | bFGF Rate of decrease (%) | TGF- β reduction (%) |
Example 1 | 33.42 | 31.53 | 37.38 |
Example 7 | 34.23 | 32.81 | 36.54 |
Comparative example 1 | 40.43 | 37.22 | 43.27 |
Comparative example 2 | 37.96 | 37.19 | 42.61 |
Comparative example 3 | 36.82 | 36.48 | 40.13 |
Comparative example 4 | 39.07 | 38.14 | 40.83 |
The results show that the combined use of the embryonic stem cell culture solution, the snake oil and the cordyceps extract can obviously reduce the reduction rate of active ingredients in the stem cell culture solution, and the combined use of the embryonic stem cell culture solution, the snake oil and the cordyceps extract can improve the protective effect on oxidative damage of skin cells and improve the anti-aging function.
Experimental example 3
The essence prepared in the example 1 and the essence prepared in the comparative examples 1 to 3 and a plurality of biological fiber mask cloths are respectively taken by adopting a conventional mask preparation method to prepare 4 masks, each mask is respectively soaked in 25ml of the essence prepared in the example 1 and the comparative examples 1 to 3, and the 4 masks are respectively used as experimental raw materials. Selecting 32 test persons with no obvious difference in skin quality and water content under the conditions of room temperature and relative humidity of 50-60%, and carrying out a facial mask performance experiment when the age is 32-38 years, wherein specifically, 8 volunteers apply facial masks prepared from the essence liquid in the embodiment 1, 8 facial masks prepared from the essence liquid in the embodiment 2, 8 facial masks prepared from the essence liquid in the embodiment 3, and the facial masks are applied once every two days for fifteen times, each application lasts for about 15 minutes, and the diet is light and consistent as much as possible in the period. Before and after fifteen trials of the sample, respectively testing the moisture content of the Skin by using a CK Multi Skin Test Center MC750 (multifunctional Skin Test Center) in a room with the temperature of 25 +/-1 ℃ and the humidity of 50% +/-5%, and testing for 3 times to take an average value; testing the facial melanin value by a Mexameter MX 18 skin melanin and blood red pigment tester, and taking an average value after 3 times of testing; the area of skin wrinkles around the eyes was measured with a Vissionline VI650 skin wrinkle tester and the percentage improvement in moisture content, percentage improvement in wrinkle area ratio, and percentage improvement in melanin were calculated before and after using the mask, and the results are shown in Table 3.
Table 3 facial mask performance test results table
The results show that compared with comparative examples 1-3, the facial mask prepared by the invention has the advantages that the moisture content of the skin is obviously improved, and the improvement conditions of wrinkles and melanin are also obviously improved.
Experimental example 4
10 men and women aged 20-35 years old were invited to observe and apply the essences prepared in examples 4, 5 and 6, and then the appearance, texture, applied touch, and applied moisture preference evaluations were performed on each sample according to the scoring criteria. The scoring standard is like-5 points, preferred-4 points, generally-3 points, less preferred-2 points, and less preferred-1 point. The results are shown in Table 4.
Table 4 satisfaction evaluation results table
As can be seen from the above table, the satisfaction degrees of the appearance and the texture of the three essences are not very different, but the satisfaction degree of the essence of example 4 is the highest in terms of the smearing touch feeling and the smearing moistening degree, which indicates that the essence of example 4 of the present invention can obtain a better skin feeling by optimizing the composition of the emollient and the humectant.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. The anti-aging composition is characterized by comprising the following raw materials in percentage by mass:
1-99 wt% of animal and plant cell extract containing cell factor;
0.01-40 wt% of snake oil;
0.01-1 wt% of cordyceps extract.
2. The anti-aging composition according to claim 1, which comprises the following raw materials in percentage by mass:
30-70 wt% of animal and plant cell extract containing cell factor;
5-25 wt% of snake oil;
0.1-0.5 wt% of cordyceps extract.
3. The antiaging composition according to claim 1, wherein the cytokine-containing animal/plant cell extract is at least one selected from the group consisting of an embryonic stem cell culture solution, an animal umbilical cord extract, a plant cell extract and a placenta extract.
4. The anti-aging composition according to claim 3, wherein the embryonic stem cell culture fluid is prepared by a method comprising the steps of:
transferring the embryonic stem cells into a serum-free culture medium for subculture, periodically replacing a culture solution, collecting the replaced culture solution, filtering, desalting and concentrating to obtain the embryonic stem cell culture solution.
5. An anti-aging formulation comprising the anti-aging composition of any one of claims 1 to 4;
the anti-aging preparation is toner, lotion, eye cream, condensation, cream, essence or facial mask.
6. The anti-aging formulation according to claim 5, comprising, in mass percent:
0.1-5 wt% of emulsifier;
24-42 wt% of water;
the anti-aging composition accounts for 35 to 80 weight percent.
8. the anti-aging formulation according to claim 7, wherein the humectant is at least one of glycerin, propylene glycol, butylene glycol, hyaluronic acid, panthenol, trehalose, and dextran; preferably, the humectant is (0.1-0.5) by mass: (1-10): (1-5) hyaluronic acid, glycerol, butylene glycol;
the emulsifier is at least one of PEG-100 stearate, potassium cetyl phosphate, glyceryl stearate and span 60;
the thickening agent is at least one of xanthan gum, carbomer and hydroxyethyl cellulose;
the emollient is at least one of GTCC, cetearyl alcohol, isopropyl palmitate, lanolin and dimethicone; preferably, the emollient is prepared from (1-6) by mass: (1-8) isopropyl palmitate with dimethicone;
the preservative is at least one of phenoxyethanol, nipagin ester, chlorphenesin and bis (hydroxymethyl) imidazolidinyl urea;
the antioxidant is at least one of tocopherol and vitamin C, BHT.
9. A method of preparing the anti-aging formulation of claim 7 or 8, comprising the steps of:
preparing an aqueous phase solution: adding the humectant and the cordyceps extract into water, dissolving and mixing uniformly, adding the emulsifier and the thickener, homogenizing until the mixture is uniformly dispersed, then adding the embryonic stem cell culture solution and the preservative, and stirring uniformly to obtain a water-phase solution;
preparing an oil phase solution: uniformly mixing an emollient, snake oil and an antioxidant to obtain an oil phase solution for later use;
an emulsification step: mixing the water phase solution and the oil phase solution, and homogenizing to obtain the final product.
10. The preparation method as claimed in claim 9, wherein in the aqueous phase solution preparation step and/or the emulsification step, the homogenization speed is 2000-5000rpm, the stirring speed is 10-50rpm, and the time is 3-10min during the homogenization.
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113197920A (en) * | 2021-05-19 | 2021-08-03 | 杭州露源生物科技有限公司 | Preparation method of embryonic stem cell culture extract for skin aging resistance |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003292430A (en) * | 2002-04-01 | 2003-10-15 | Noevir Co Ltd | Skin care preparation |
CN105560157A (en) * | 2015-12-29 | 2016-05-11 | 徐跃 | Novel application of Cordyceps fungus extract |
CN107334816A (en) * | 2017-05-08 | 2017-11-10 | 张友明 | A kind of skin scald agent |
CN107988152A (en) * | 2017-11-10 | 2018-05-04 | 武汉北度生物科技有限公司 | The separation method of oxidation-resistant active ingredient in human umbilical cord mesenchymal stem cells nutrient solution |
CN108354856A (en) * | 2018-04-02 | 2018-08-03 | 佛山市芊茹化妆品有限公司 | A kind of snake oil anti-apolexis composition and its application in cosmetics |
CN109276531A (en) * | 2018-11-09 | 2019-01-29 | 东阿阿胶股份有限公司 | A kind of facial mask and its preparation process containing donkey intacellin |
-
2019
- 2019-12-26 CN CN201911365251.9A patent/CN110974770A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003292430A (en) * | 2002-04-01 | 2003-10-15 | Noevir Co Ltd | Skin care preparation |
CN105560157A (en) * | 2015-12-29 | 2016-05-11 | 徐跃 | Novel application of Cordyceps fungus extract |
CN107334816A (en) * | 2017-05-08 | 2017-11-10 | 张友明 | A kind of skin scald agent |
CN107988152A (en) * | 2017-11-10 | 2018-05-04 | 武汉北度生物科技有限公司 | The separation method of oxidation-resistant active ingredient in human umbilical cord mesenchymal stem cells nutrient solution |
CN108354856A (en) * | 2018-04-02 | 2018-08-03 | 佛山市芊茹化妆品有限公司 | A kind of snake oil anti-apolexis composition and its application in cosmetics |
CN109276531A (en) * | 2018-11-09 | 2019-01-29 | 东阿阿胶股份有限公司 | A kind of facial mask and its preparation process containing donkey intacellin |
Non-Patent Citations (3)
Title |
---|
李孟心 等: "Nrf2 HO-1信号通路在人诱导性多能干细胞氧化应激中的作用", 《中国生物化学与分子生物学报》 * |
王建新 等: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
陈浩宏: "《活力自然美 能量美容俏佳人》", 31 January 2013, 上海科学技术出版社 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113197920A (en) * | 2021-05-19 | 2021-08-03 | 杭州露源生物科技有限公司 | Preparation method of embryonic stem cell culture extract for skin aging resistance |
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