CN110840817A

CN110840817A - Antibacterial mask liquid with skin quality improving effect

Info

Publication number: CN110840817A
Application number: CN201911379778.7A
Authority: CN
Inventors: 韩卫星
Original assignee: Guangdong Baioufei Silk Cell Research Center Co Ltd
Current assignee: Guangdong Baioufei Silk Cell Research Center Co Ltd
Priority date: 2019-12-27
Filing date: 2019-12-27
Publication date: 2020-02-28

Abstract

This scheme relates to cosmetics technical field. More particularly, the scheme relates to an antibacterial mask liquid with the effect of improving skin quality, and the mask liquid comprises 1-5% of caragana microphylla leaf extract, 1-3% of holly tree extract and 0.5-2% of myricetin by weight percentage. Is easy to be absorbed by skin, can accelerate self-renewal and repair of skin cells, and has effects of whitening skin, keeping moisture, resisting aging, resisting wrinkle, removing mottle, etc. Meanwhile, the skin quality can be rapidly improved, the skin elasticity can be improved, wrinkles can be smoothed, and the like. Because of having certain bacteriostatic effect, the stimulation of adding chemical components to the skin is avoided.

Description

Antibacterial mask liquid with skin quality improving effect

Technical Field

This scheme relates to cosmetics technical field. More particularly, the present invention relates to an antibacterial mask liquid having an effect of improving skin texture.

Background

The skin is one of the important organs of the human body and is an important mark for the health and beauty of the human body, and the face is the main skin part exposed outside and becomes the most main window for showing the skin quality. With the development of society and the progress of science and technology, the pressure of people on work and life is greater and greater, and the problem of environmental pollution is more and more serious. The long-term life of the skin care product in the environment with fast pace, irregular diet and great pollution causes the continuous weakening of the skin tolerance of people, the sensitive and damaged skin conditions are more and more serious, and more people have sub-health skin. Therefore, skin care has gradually become an indispensable part of human life.

For improving the facial skin, the facial mask has the advantages of remarkable skin improving effect and convenience in use. In a common facial mask, facial mask solution is soaked on non-woven fabric, then the facial mask is covered on the face for a certain time, and the facial skin is maintained and repaired by the contact of the facial mask solution and the facial skin. The components of the mask liquid directly influence the improvement effect of the mask on the skin. The mask liquid in the existing mask adopts more chemical components, on one hand, certain irritation exists on the skin, the phenomena of skin allergy and the like are easily caused, and on the other hand, the existing mask has uneven quality and has an insufficient obvious effect on resisting wrinkles and ageing on the face. Based on the above, the novel anti-wrinkle, anti-aging and moisturizing product is different from competitor products and can achieve a certain effect. There is a need to develop a product that is distinguishable from the prior art.

The plant extract is extracted from natural plants, and contains alkaloid, flavonoid, organic acid, saponin and other active substances with antibacterial, antiinflammatory and antipruritic effects and no side effects. Therefore, facial mask solutions containing plant extracts are becoming more and more popular with beauty lovers.

Disclosure of Invention

The technical scheme aims to solve the problems of the existing facial mask and provide an antibacterial facial mask liquid with the effect of improving the skin quality of the skin in order to develop a new product.

The technical scheme adopted by the scheme for solving the technical problem is as follows: an antibacterial facial mask liquid with skin-improving effect comprises (by weight) Caragana microphylla leaf extract 1-5%, folium Ilicis Cornutae Immaturum extract 1-3%, and myricetin 0.5-2%.

Preferably, the feed also comprises cuttlefish bone meal 2-5%. Is a regenerative tissue material and has good effect on repairing skin.

Preferably, the facial mask liquid comprises 3% of caragana microphylla leaf extract, 1.5% of Chinese holly leaf extract, 0.5-2% of myricetin and 3% of cuttlefish bone meal in percentage by weight.

The caragana microphylla leaf extract, the paper mulberry extract and the myricetin are combined for the facial mask liquid for facial care for the first time, and the caragana microphylla leaf extract, the paper mulberry extract and the myricetin are added simultaneously, so that the composition has a synergistic effect, and has the effects of whitening, resisting aging and preventing spots. The caragana microphylla leaf extract contains more flavonoids, the Chinese holly leaf extract contains various soothing anti-allergy, anti-inflammatory and antibacterial, whitening and freckle-removing components, and the Chinese holly leaf extract can regulate skin immunity from endogenesis and enhance the self-repairing capability of skin under the combined action of other components of the facial mask liquid, so that the effects of whitening, moisturizing, soothing anti-allergy, anti-inflammatory and antibacterial and the like are achieved. The myricetin can inhibit various enzymes, has an antioxidation effect, and is more remarkable in anti-wrinkle and anti-aging aspects when being matched with other components.

Preferably, the mask liquid further comprises, by weight, 0.05-0.2% of tremella polysaccharide, 0.5-0.8% of green pine needle extract and 50.3-0.7% of vitamin B. The tremella polysaccharide has strong antiallergic effect, can prevent stimulation of other components to facial pores, the pine needle extract is extracted from pine needle leaves, contains lignans, volatile oils, crude protein, amino acids, pigments, vitamins, polyphenols, fatty acids, flavones, glycosides, terpenes and other chemical book active ingredients, has wide pharmacological action and pharmacological action in the aspects of anti-aging and the like, and the vitamin B5 can promote normal keratinization of skin and improve the tolerance of the skin to surfactants. Balancing the discomfort caused by the sensitivity of other ingredients. The combination of the three components has better effect on the elastic recovery of the skin.

Preferably, the mask liquid further comprises, by weight, 0.01% of tremella polysaccharide, 0.8% of green pine needle extract and 50.7% of vitamin B.

Preferably, the facial mask liquid also comprises 5-8% of propylene glycol, 10-20% of glycerol, 0.5-2% of aloe barbadensis extract, 0.3-0.8% of oat β -glucan, 30.5-1.5% of ceramide, 0.1-0.3% of dipotassium glycyrrhizinate, 0.5-1.8% of sodium hyaluronate, 0.05-1.2% of pH regulator and the balance of water by weight percentage.

Preferably, the caragana microphylla leaf extract is extracted by the following method: freeze drying Caragana Korshinskii kom leaves, pulverizing at 0-4 deg.C to below 100 mesh in a low temperature ultramicro pulverizer to obtain powder, mixing the powder with deionized 90% ethanol solution at a ratio of 1g powder to 8mL solution, adding sodium bicarbonate with a mass of 1.3% of the powder, heating to reflux and extracting for 1-3 times, filtering to remove impurities, and vacuum concentrating the filtrate under reduced pressure to obtain Caragana Korshinskii kom leaf extract.

Preferably, the preparation method of the paper mulberry extract comprises the following steps: (1) selecting paper mulberry flowers, removing impurities, cleaning, freeze-drying and crushing into 80-120 meshes, (2) extracting the powder with an ethanol solution with the mass concentration of 80-85% according to the proportion of 1g/5-6ml, assisting with ultrasonic treatment, wherein the extraction time is 1-3h, blowing carbon dioxide into an extracting solution during extraction, and adding 3-5% by weight of sodium acetate into the extracting solution; (3) decolorizing the extractive solution, and vacuum concentrating under reduced pressure to obtain folium Ilicis Cornutae Immaturum extract.

The beneficial effect of this scheme is: is easy to be absorbed by skin, can accelerate self-renewal and repair of skin cells, and has effects of whitening skin, keeping moisture, resisting aging, resisting wrinkle, removing mottle, etc. Meanwhile, the skin quality can be rapidly improved, the skin elasticity can be improved, wrinkles can be smoothed, and the like. Because of having certain bacteriostatic effect, the stimulation of adding chemical components to the skin is avoided.

Detailed Description

The technical solution of the present invention will be further specifically described below by way of specific examples. In the present invention, the raw materials and equipment used are commercially available or commonly used in the art, unless otherwise specified. The methods in the following examples are conventional in the art unless otherwise specified.

Example 1 an antibacterial mask solution having skin-condition improving effects, which comprises, by weight, 1-5% of caragana microphylla leaf extract, 1-3% of holly tree extract, 0.5-2% of myricetin, 2-5% of cuttlefish bone meal, 5-8% of propylene glycol, 10-20% of glycerin, 0.5-2% of aloe barbadensis extract, 0.3-0.8% of oat β -dextran, 30.5-1.5% of ceramide, 0.1-0.3% of dipotassium glycyrrhizinate, 0.5-1.8% of sodium hyaluronate, 0.05-1.2% of a pH adjusting agent, and the balance water.

Example 2 an antibacterial mask solution having skin texture improving effects, which comprises, by weight, 3% of caragana microphylla leaf extract, 1.5% of holly tree extract, 0.5-2% of myricetin, 3% of cuttlefish bone meal, 5-8% of propylene glycol, 10-20% of glycerin, 0.5-2% of aloe vera extract, 0.3-0.8% of oat β -dextran, 30.5-1.5% of ceramide, 0.1-0.3% of dipotassium glycyrrhizinate, 0.5-1.8% of sodium hyaluronate, 0.05-1.2% of pH adjusting agent, and the balance water.

Example 3: on the basis of the embodiment 1, the mask liquid further comprises, by weight, 0.05-0.2% of tremella polysaccharide, 0.5-0.8% of green pine needle extract and 50.3-0.7% of vitamin B.

Example 4: on the basis of embodiment 2, the mask liquid further comprises, by weight, 0.01% of tremella polysaccharide, 0.8% of green pine needle extract and 50.7% of vitamin B.

The extraction method of the caragana microphylla leaf extract used in the scheme is as follows: freeze drying Caragana Korshinskii kom leaves, pulverizing at 0-4 deg.C to below 100 mesh in a low temperature ultramicro pulverizer to obtain powder, mixing the powder with deionized 90% ethanol solution at a ratio of 1g powder to 8mL solution, adding sodium bicarbonate with a mass of 1.3% of the powder, heating to reflux and extracting for 1-3 times, filtering to remove impurities, and vacuum concentrating the filtrate under reduced pressure to obtain Caragana Korshinskii kom leaf extract.

The preparation method of the paper mulberry extract used in the scheme comprises the following steps: (1) selecting paper mulberry flowers, removing impurities, cleaning, freeze-drying and crushing into 80-120 meshes, (2) extracting the powder with an ethanol solution with the mass concentration of 80-85% according to the proportion of 1g/5-6ml, assisting with ultrasonic treatment, wherein the extraction time is 1-3h, blowing carbon dioxide into an extracting solution during extraction, and adding 3-5% by weight of sodium acetate into the extracting solution; (3) decolorizing the extractive solution, and vacuum concentrating under reduced pressure to obtain folium Ilicis Cornutae Immaturum extract.

The antioxidant activity of the essence is measured by DPPH method.

Accurately weighing DPPH reagent mg, dissolving with anhydrous ethanol, quantitatively transferring into 10mL volumetric flask, adding anhydrous ethanol to constant volume to scale, taking into volumetric flask, shaking to obtain DPPH stock solution with concentration of 0.1 mmol/L

Dissolving DPPH 1mg in about 20mL ethanol solvent, performing ultrasonic treatment for 5min, and shaking thoroughly and uniformly. 1mL of this DPPH solution was taken, and the value of A was measured at 519nm so that A became 1.2 to 1.3.

Adding 4.0mL of PPH solution and sample essence into a 10mL test tube in sequence, adding absolute ethyl alcohol to scale, immediately mixing, measuring a light absorption value (A) at a wavelength of 517nm by using a 1cm cuvette, marking the light absorption value as Ai, then measuring the light absorption value after storing in a greenhouse in a dark place for 30min, marking the light absorption value as Aj, and taking an ethanol solution only added with DPPH as a control test, and marking the light absorption value as Ac. The radical clearance (K) was calculated as follows:

K(%)=[1-(Ai-Aj)/Ac]*100%

the test was repeated three times, and the average was taken as the final result, and the results are shown in Table 1.

Determination of tyrosinase activity inhibiting ability:

3mL of 0.05% L-tyrosine, 4mL of phosphate buffer solution with pH 6.8 and 0.3 mL of sample solution are added into a 10mL centrifuge tube at a time, the mixture is shaken and heated in a water bath at 37 ℃ for 10min, 1mL of 500U/mL tyrosinase is added and heated for 5min, centrifugation is carried out (12000rpm, 5min), the absorbance A1 is measured at 475nm, and the absorbance A2 is measured when the phosphate buffer solution is used for replacing the sample solution. And calculating the inhibition rate of the sample solution on tyrosinase. The inhibition ratio of tyrosinase is (A2-A1)/A2, and the results are shown in Table 1.

Determination of the Activity of superoxide dismutase (SOD):

the experiment adopts a photochemical reduction method, and the principle is as follows: under the condition of the existence of methionine and riboflavin, the nitro blue tetrazole generates photochemical reduction reaction after being irradiated by light to generate blue methyl hydrazone, and the blue methyl hydrazone has maximum light absorption at 560 nm. SOD can inhibit photochemical reduction of NBT, and the inhibition intensity is in direct proportion to enzyme activity in a certain range.

Reagent:

1.50 mmol/l Phosphate Buffer (PBS) pH 7.8. Containing 0.1 mol/l EDTA

2.220m mol/l methionine: 3.2824g methionine was weighed out and dissolved to 100ml (ready for use) in 50 mmol/l phosphate buffer pH 7.8.

3.1.25m mol/l Nitro-tetrazolium chloride (NBT) solution (ready-made): NBT (0.102 g) was weighed out and dissolved in 50 mmol/l Phosphate Buffer Solution (PBS) pH7.8 to 100 ml.

4.0.033m mol/l riboflavin: 2.52mg was weighed out and dissolved in PBS to a volume of 200ml (stored in a dark place).

The steps are carried out according to the conventional procedure of the method.

	Free radical clearance/%)	Tyrosinase inhibition%	Superoxide dismutase inhibition%
				Example 1	87.4	82.7	80.8
Example 2	88.1	81.4	82.7
				Example 3	92.3	85.9	87.2
Example 4	93.6	87.1	88.3

The result shows that the facial mask liquid provided by the invention has obvious free radical scavenging capacity and can effectively inhibit the activity of tyrosinase and the activity of superoxide dismutase.

Skin elasticity test: taking 20g of the facial mask liquid obtained in the examples 1-4, and soaking the facial mask liquid through non-woven fabric to obtain a facial mask; 150 female volunteers with mild skin aging and no previous skin care cosmetic allergy history are selected, the female volunteers are 30-45 years old, and are randomly divided into 5 groups of 30 persons in each group according to a parallel comparison principle. A blank test is carried out on facial skin 20min after facial cleaning of the volunteers by using a German CK skin elasticity tester MPA580, a blank value is determined, then facial masks prepared by the facial mask liquid of the embodiment 1-4 and commercially available facial masks of a certain brand are respectively applied to all groups of volunteers, 1 time per day and one time, facial cleaning is carried out before application, and the application time is 20 min. Skin elasticity test was performed on volunteers at 5d, 10d, 15d and 20d after the start of application of the mask, and the test time was 20min after completion of the application of the mask. The rate of increase in skin elasticity was calculated.

Skin elasticity test table:

	5d	10d	15d	20d
					example 1	11.52%	25.48	28.31％	36.88％
Example 2	9.89%	23.69％	31.78％	37.27％
					Example 3	10.25%	24.88％	32.13％	36.96％
Example 4	9.63%	26.61％	29.58％	35.51％
					Comparative example 1	6.54%	10.28％	13.49％	18.53％

From the above test results, it can be seen that: the facial mask prepared by the facial mask liquid disclosed by the invention has a good effect of increasing the elasticity of facial skin, and the skin aging performance is remarkably improved, which shows that the facial mask liquid disclosed by the invention has remarkable effects of increasing the elasticity of the skin, tightening and removing wrinkles. The skin elasticity has close relationship with the moisture content of the horny layer, the moisture content of the horny layer is high, and the skin elasticity is good.

The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit of the invention as set forth in the claims.

Claims

1. An antibacterial mask liquid with the effect of improving skin quality is characterized in that: the facial mask liquid comprises 1-5% of caragana microphylla leaf extract, 1-3% of holly tree extract and 0.5-2% of myricetin by weight percentage.

2. The mask liquid according to claim 1, characterized in that: the mask liquid also comprises 2-5% of cuttlefish bone meal according to the weight percentage.

3. The antibacterial mask liquid with the skin-texture improving effect according to claim 2, is characterized in that: the facial mask liquid comprises 3% of caragana microphylla leaf extract, 1.5% of Chinese holly leaf extract, 0.5-2% of myricetin and 3% of cuttlefish bone meal.

4. The antibacterial mask liquid with the skin-texture improving effect according to claim 3, is characterized in that: the mask liquid also comprises, by weight, 0.05-0.2% of tremella polysaccharide, 0.5-0.8% of green pine needle extract and 50.3-0.7% of vitamin B.

5. The antibacterial mask liquid with the skin-texture improving effect according to claim 3, is characterized in that: the mask liquid also comprises 0.01 percent of tremella polysaccharide, 0.8 percent of green pine needle extract and 50.7 percent of vitamin B according to weight percentage.

6. The antibacterial mask liquid with the skin quality improving effect according to claim 3, wherein the mask liquid further comprises, by weight, 5-8% of propylene glycol, 10-20% of glycerin, 0.5-2% of aloe barbadensis extract, 0.3-0.8% of oat β -glucan, 30.5-1.5% of ceramide, 0.1-0.3% of dipotassium glycyrrhizinate, 0.5-1.8% of sodium hyaluronate, 0.05-1.2% of pH regulator and the balance of water.

7. The antibacterial mask liquid with the skin-texture improving effect according to claim 1, is characterized in that: the extraction method of the caragana microphylla leaf extract comprises the following steps: freeze drying Caragana Korshinskii kom leaves, pulverizing at 0-4 deg.C to below 100 mesh in a low temperature ultramicro pulverizer to obtain powder, mixing the powder with deionized 90% ethanol solution at a ratio of 1g powder to 8mL solution, adding sodium bicarbonate with a mass of 1.3% of the powder, heating to reflux and extracting for 1-3 times, filtering to remove impurities, and vacuum concentrating the filtrate under reduced pressure to obtain Caragana Korshinskii kom leaf extract.

8. The antibacterial mask liquid with the skin-texture improving effect according to claim 1, is characterized in that: the preparation method of the paper mulberry extract comprises the following steps: (1) selecting paper mulberry flowers, removing impurities, cleaning, freeze-drying and crushing into 80-120 meshes, (2) extracting the powder with an ethanol solution with the mass concentration of 80-85% according to the proportion of 1g/5-6ml, assisting with ultrasonic treatment, wherein the extraction time is 1-3h, blowing carbon dioxide into an extracting solution during extraction, and adding 3-5% by weight of sodium acetate into the extracting solution; (3) decolorizing the extractive solution, and vacuum concentrating under reduced pressure to obtain folium Ilicis Cornutae Immaturum extract.