WO2015078398A1 - Chinese herbal medicine compound for repairing ultraviolet damage, and uses thereof in cosmetics - Google Patents

Abstract

Uses of a peach blossom and peach seed compound extract for repairing ultraviolet damage and promoting energy metabolism, and uses of the peach blossom and peach seed compound extract as a cosmetic additive having a function of repairing ultraviolet damage and promoting energy metabolism.

Description

Chinese herbal compound for ultraviolet damage repair and its application in cosmetics Technical field

The invention relates to a Chinese herbal compound for ultraviolet damage repair and its application in cosmetics, in particular to a peach peach kernel compound and its application in cosmetics.

Background technique

Ultraviolet rays not only damage the outermost skin cells, but also cause dermal cell necrosis and metabolic disorders. The impurities in the cells cannot be metabolized, causing sunburn, loss of elasticity, premature aging, sensitivity, wrinkles, and skin. After the injury, the tingling and itching are unbearable, scratching the bleeding, the skin's own resistance to disease and resistance is weakened, and even edema, exudation, formation of folliculitis, sputum and other difficult to cure skin diseases.

At present, the understanding of the mechanism of ultraviolet light on skin damage includes oxidative stress, inflammatory reaction, apoptosis, degradation of collagen and elastin. When the skin receives excessive ultraviolet radiation, the skin tissue cells will produce oxidative stress, produce excessive reactive oxygen species ROS, destroy the integrity of the cell membrane and the anti-oxidation system inside the cell, further causing cell inflammation, apoptosis and tumor formation. . At the same time, excessive ultraviolet radiation causes skin collagen, hyaluronic acid and elastin to degrade, resulting in elastic degeneration, roughness and wrinkles. Therefore, most of the repair methods after the ultraviolet ray damage are based on these mechanisms, and substances having antioxidation, anti-inflammatory, and matrix metalloproteinase inhibition activities are used. For example, ash tree extract effectively removes a large number of free radicals generated in human Fb cells after UVB irradiation, and reduces the expression of MMP-1 mRNA and protein, thereby resisting skin damage after UVB irradiation; for example, marigold flower extract contains β Carotene, which inhibits the production of wrinkles by inhibiting the activity of hyaluronidase, elastase, and MMP-1, thereby exerting an ultraviolet protection effect.

ATP is a key molecule in the metabolism of cell energy. It is the main energy source that skin cells can directly use. It is also an important indicator of the healthy viability of cells. The reduction of ATP leads to the reduction of energy metabolism, and the corresponding functional decline. The most obvious is skin aging, dull acne and so on. New research has found that intense exposure to ultraviolet light can cause a decrease in ATP production, leading to a decrease in energy metabolism and affecting the normal function of skin cells. The increase of ATP can promote the increase of cells after injury, and promote Into the metabolism of cell energy, which is conducive to the repair of skin damage caused by ultraviolet radiation.

Peach blossom is the flower of the Prunus persica (L.) Batsch of the Rosaceae plant. The bitterness is flat and the water is swollen. The "Shen Nong's Herbal Classic" records that the peach blossom is "a good color"; the peach kernel is the dry ripe of the Prunus persica (L.) Batsch or the Prunus davidiana (Carr.) Franch. seed. It tastes bitter and sweet, promotes blood circulation, and can moisturize dryness. The two are compatible, activating blood circulation, moistening dryness and diuresis, and can make blood stasis, water and swollen body restore blood and blood circulation, and eliminate qi and blood stasis in the body.

The prior art describes the research progress of the skin damage mechanism of ultraviolet rays and natural products having ultraviolet protection. The research progress of the skin damage mechanism of ultraviolet light and the UV-protectant of natural sources is reviewed. Specifically pointed out: long-term exposure of the skin to ultraviolet light can cause skin damage, skin photoaging and even skin cancer and other skin lesions. The main mechanisms of photoaging caused by ultraviolet light are: oxidative stress, inflammatory reaction, apoptosis, degradation of collagen and elastin. At present, the focus of attention at home and abroad is the development of UV protective agents. At present, flavonoids, terpenoids, tannins and coumarins are mainly studied. However, none of the existing reports mention that the promotion of energy metabolism and the synthesis of ATP can also repair UVB.

Chinese patent application 201110198166.5 discloses a compound Chinese medicine extract and its application in whitening moisturizing anti-aging skin care products. This document mainly discloses a compound Chinese medicine extract and its application in whitening moisturizing anti-aging skin care products. The compound Chinese medicine extract is prepared from the following raw materials by mass: 10 to 50 parts of Selaginella, 10 to 50 parts of peach, 10 to 50 parts of white and 10 to 50 parts of notoginseng. The compound Chinese herbal extract obtained by the above raw materials has an inhibitory effect on the proliferation of tyrosinase and melanocytes, has an excellent whitening effect, and has a promoting effect on proliferation of fibroblasts; in epidermal cell culture, the above-mentioned compound Chinese herbal extracts The production of ceramide has a good promotion effect. The compound Chinese medicine extract is applied to prepare a whitening moisturizing anti-aging skin care product, and the obtained whitening moisturizing anti-aging skin care product has the lasting natural whitening effect, and can improve the metabolism and moisturizing function of the skin. This patent application, while also revealing a composition, is not a composition of the peach kernel of the present invention.

Chinese patent application 201210312256.7 discloses a mulberry leaf spotting beauty tea and a preparation method thereof. Specifically, a mulberry leaf spotting beauty tea and a preparation method thereof are provided, and various raw materials are: mulberry leaves, peach blossoms, raspberries, alfalfa, spatholobus, codonopsis, perrin, gynostemma, angelica, polygonatum, peach kernel, turmeric, incense Attachment, white peony root, Chuanxiong, Danshen, Coix seed, Atractylodes, Peach blossom, White and, Ligustrum lucidum, Campsis, white peony. The invention solves the defects that the mulberry leaf tea is easy to be stuffy yellow, brittle, and the soup color is red, and the polyphenols in the mulberry leaves are exhausted. It may be converted into amino acid, which is beneficial to remove the bitter taste in tea and make it more delicious. The mulberry leaf contains mulberry glycoside F, which has inhibitory effect on the biosynthesis of melanin. It is confirmed that mulberry leaf juice has anti-oxidation effect, non-toxic, no Chemical pollution, can be used for facial acne, pigmentation, phlegm, pharyngeal, hair growth, hair care, liver and eyesight treatment, plus some Chinese herbal medicines that promote blood circulation and phlegm, most of these herbs are light, do not affect mulberry tea The taste is easy to accept, and it can better achieve the beauty and remove the pigmentation. Similarly, the application is directed to mulberry leaf compositions, although peach and peach kernels are added to it, but it is not a major Chinese herbal ingredient.

It can be seen that the prior art does not mention the role of the peach peach kernel compound in repairing UVB radiation damage and promoting energy metabolism. The present invention finds for the first time that the peach peach kernel compound has the properties of ultraviolet damage repair and promoting skin cell energy metabolism.

Summary of the invention

The invention aims to provide a Chinese herbal compound extract with ultraviolet damage repair, which can promote ATP synthesis and repair ultraviolet damage caused by UVB. At the same time, it can be added as an efficacy additive to cosmetics for skin care after UV injury.

The extraction process of peach peach kernel is as follows:

Peach medicinal materials are soaked in a suitable solvent for about 0.5-1 h, and extracted by the corresponding method. The filtrate is taken, statically filtered, concentrated, and dried to form a powder.

The peach kernel is soaked in a suitable solvent for about 0.5-1 h, and extracted by the corresponding method. The filtrate is taken, statically filtered, concentrated, and dried to form a powder.

The peach blossom extract and the peach kernel extract were mixed in proportion, and finally a 1% by weight composite extract solution was prepared.

The Chinese herbal medicines in the formula may be appropriately enlarged or reduced according to the proportion; suitable solvents include water (distilled water, deionized water, purified water), different concentrations of ethanol solution, different concentrations of polyol solution and other solvents of similar polarity; the extraction method may It is a method of boiling, reflux extraction, dipping, seepage, and the like.

The preparation of the compound extract of Peach Blossom Peach can also be carried out by supercritical extraction, subcritical extraction and organic solvent extraction. Solvents for organic solvent extraction include n-hexane, ethyl acetate, diethyl ether, petroleum ether, Medium and low polarity solvents such as acetone, and different concentrations of ethanol, methanol, and polyols. The extraction method may be a method such as reflux extraction, impregnation, or osmosis. Moreover, ultrasonic or microwave methods can also be used for assisting during the extraction process.

In a first aspect, the present invention relates to the use of a peach peach kernel compound extract for the repair of ultraviolet damage and the promotion of energy metabolism.

In a second aspect, the present invention relates to the use of a peach peach kernel compound extract as an additive having ultraviolet damage repair and promoting energy metabolism in cosmetics.

In one embodiment, the peach peach kernel compound extract is used in an amount of 0.00001 to 100% by weight. In another embodiment, the peach peach kernel compound extract is used in an amount of 0.0001 to 50% by weight. In a preferred embodiment, the peach peach kernel compound extract is used in an amount of from 0.001% to 20% by weight.

The cosmetic of the present invention is selected from the group consisting of facial cleansers, lotions, lotions, creams, gels, masks, shampoos, shower gels, and hair conditioners. In one embodiment, the cosmetic of the present invention comprises a peach peach kernel compound extract and an acceptable adjuvant.

In one embodiment, the cosmetic comprises from 0.00001 to 100% by weight of a peach peach kernel compound extract. In another embodiment, the cosmetic comprises 0.0001 to 50% by weight of a peach peach kernel compound extract. In another embodiment, the cosmetic comprises from 0.001% to 20% by weight of the peach peach kernel compound extract.

DRAWINGS

Figure 1 shows the percentage of ATP content of normal cells, UV irradiated cells, and various concentrations of the peach extract of the present invention. In the figure, # indicates a significant difference in the UVB irradiation group with respect to the normal group; ** indicates a significant difference (p < 0.01) with respect to the UVB irradiation group.

detailed description

At present, most of the repairs after UV damage provide solutions from the perspectives of oxidative stress, DNA repair, inflammation, apoptosis, collagen and elastin degradation. There is no solution for UV damage repair from the perspective of ATP energy metabolism. The invention firstly proposes that the peach peach kernel compound has the effects of ultraviolet damage repair and energy metabolism. Peach blossom peach kernel complex provided by the invention The extract can repair the energy loss of cells after UVB irradiation, promote the energy metabolism of skin cells and the synthesis of ATP, which is beneficial to the repair of UV damage.

After extensive and intensive research, the inventors of the present invention found that the peach peach kernel compound extract has ultraviolet damage repair and promotes energy metabolism.

Therefore, the peach peach kernel compound extract can be added to cosmetics as an efficacy additive.

None of the literatures involved in the peach peach kernel compound extract has UV damage repair and promote energy metabolism, and it has not been mentioned as a functional additive added to cosmetics. Therefore, the present invention firstly proposes that the peach peach kernel compound extract has ultraviolet damage repair and promotes energy metabolism, and can be added as a functional additive to cosmetics for ultraviolet damage repair and skin cell energy supplementation.

In cosmetic applications, the peach peach kernel compound extract can be in various forms. For example, the application form of the peach peach kernel compound extract may be a cleansing lotion, a lotion, a lotion, a cream, a gel, a mask, a shampoo, a shower gel, a conditioner, and the like which can be applied to human skin.

Other ingredients other than the peach peach kernel extract may be any ingredients required for a cosmetic formulation, such as: water, ethanol, butylene glycol, propylene glycol, oils, emulsifiers, thickeners, preservatives, etc.; Compound functions, such as: adding sunscreen ingredients to promote energy metabolism, sunscreen cosmetics, adding oil control ingredients to promote energy metabolism, oil control cosmetics, adding moisturizing ingredients to promote energy metabolism, moisturizing cosmetics, adding whitening ingredients to promote energy metabolism, whitening cosmetics, adding anti- The aging component becomes an anti-aging cosmetic that promotes energy metabolism and the like.

In general, the peach peach kernel compound extract can be used at a concentration of 0.00001-100% by weight. Preferably, the peach peach kernel compound extract can be used at a concentration of 0.0001 to 50% by weight. In one embodiment of the present invention, the peach peach kernel compound extract is used at a concentration of 0.00001 to 100% by weight. In another embodiment, the peach peach kernel compound extract has a concentration of from 0.001% to 50% by weight. In one embodiment, the peach peach kernel compound extract is used at a concentration of from 0.001% to 20% by weight.

In one embodiment, the peach peach kernel compound extract is used at a concentration of from 0.001 to 100 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of 0.001-50 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of 0.001 to 10 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of from 0.01 to 10 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of 0.1-10 ug/ml. In another embodiment, the peach peach kernel compound extract is used at a concentration of 1-10 ug/ml.

The peach peach kernel compound extract can be added to various cosmetics in a ratio of 0.00001-100% by weight. For example, the peach peach kernel compound extract may be used in an amount of 0.00001 to 25% by weight, for example 0.0001 to 50% by weight, for example 0.001 to 75% by weight, for example 0.1 to 100% by weight. In a preferred embodiment, the peach peach kernel compound extract in the cosmetic is used in an amount of 0.0001 to 50% by weight. In another preferred embodiment, the peach peach kernel compound extract is used in the cosmetic in an amount of 0.001 to 20% by weight.

The weight ratio of the peach flower extract and the peach kernel extract in the peach peach kernel compound extract may be any ratio. For example, in one embodiment, the weight ratio of peach blossom extract and peach kernel extract in the peach peach kernel compound extract is 3:7.

The invention is further illustrated by the following specific examples. However, it is to be understood that the examples are not intended to limit the scope of the invention. The test methods which do not specify the specific conditions in the following examples are usually carried out according to conventional conditions or according to the conditions recommended by the manufacturer. All percentages and parts are by weight unless otherwise indicated.

Example 1: Repair of skin cell energy damage caused by UVB irradiation of peach peach kernel

Experimental sample preparation:

Weigh the appropriate amount of Peach Blossom, extract it by 15 times deionized water → filter, concentrate the filtrate, and dry it into powder to obtain Peach Blossom Extract.

Weigh the appropriate amount of the peach kernel, soak it in 12 times ethanol overnight → filter, concentrate the filtrate, and dry it into powder to obtain the peach kernel extract.

Three portions of the peach blossom extract were taken, and another 7 parts of the peach kernel extract were taken and mixed in proportion, and finally a composite extract containing 1% by weight was prepared.

A 1% by weight peach peach kernel extract was removed and diluted to 10 ug/ml, 1 ug/ml, and 0.1 ug/ml with PBS buffer to obtain a sample solution.

experimental method:

1) The cells are resuscitated, and when the growth state is good, the cells are synchronized.

2) After the synchronization, the adjusted concentration is inoculated into the cell plate. The next day, the cells were placed under a UVB irradiation apparatus, and normal medium and medium containing the extract of the example were added to continue the culture incubation.

3) After incubation for a period of time, add the test solution in the kit to each well, shake, and stabilize the fluorescence at room temperature. The instrument will detect the reading.

Experimental results:

The fluorescence unit RLU measured in each well was recorded, and the average of 3 replicate wells in each group was averaged. The experimental results were subjected to t test, P<0.05, indicating significant difference; p<0.01 indicates that the difference was very significant. At the same time, the percentage value of ATP content of each unit of fluorescence was calculated based on 100% of normal cells.

The results showed that the compound extract of Peach Blossom Peach has UV damage repair and promoted cell energy metabolism. Even at low concentrations (for example, 0.1 ug / ml concentration), Peach Blossom Peach Compound extract has a good repair effect on cell energy loss after UVB UV irradiation, promotes the synthesis of ATP, and maintains the energy metabolism level of skin cells. As shown in Table 1 and Figure 1, the repair rate can reach more than 75% at 10ug/ml, and the repair rate can reach nearly 50% at a low dose concentration of 0.1ug/ml.

Table 1: UV damage repair of peach peach kernel compound extract

Peach peach kernel extract concentration	Protection rate (%)
		10ug/ml	76.43%
1ug/ml	59.19%
		0.1ug/ml	48.15%

Example 2: Preparation of energy metabolism anti-aging cream

An energy metabolism anti-aging cream comprising the peach peach kernel compound extract of the present invention was prepared according to the ingredients and amounts of Table 2.

Table 2

ingredient	% concentration (weight)
		Peach Blossom Peach Extract	1.0
Glyceryl stearate citrate	4.0
		Glycerol stearate	2.0
Cetearyl alcohol	2.0
		mineral oil	10.0
Caprylic/capric triglyceride	5.0
		1,3-butanediol	7.0
Preservatives, flavors	Moderate amount

water

To 100.0

The preparation method comprises the steps of: dissolving the peach peach extract with 1,3-butanediol, adding it to water, and stirring uniformly to obtain an aqueous phase. Add glyceryl stearate citrate, glyceryl stearate, cetearyl alcohol, caprylic/capric triglyceride, mineral oil to the oil phase pot, heat to 75 ° C -80 ° C, stir evenly Oil phase. The oil phase is slowly added to the water phase, stirring is continued, and the preservative and the essence are added after being evenly mixed, and the mixture is uniformly obtained.

Example 3: Promoting energy metabolism Preparation of moisturizing water

The energy-enhancing moisturizing water containing the peach peach kernel compound extract of the present invention was prepared according to the ingredients and amounts of Table 3.

table 3

ingredient	% concentration (weight)
		Peach Blossom Peach Extract	2.0
1,3-butanediol	8.0
		Preservative, water-soluble flavor	Moderate amount
water	To 100

Preparation method: first dissolve the peach peach kernel extract with 1,3-butanediol, then add to the water, stir, evenly add the preservative and flavor, and stir evenly.

Claims (10)

1. The use of peach peach kernel compound extract in the repair of ultraviolet damage and the promotion of energy metabolism.
2. The use of peach peach kernel compound extract as an additive with ultraviolet damage repair and energy metabolism is used in cosmetics.
3. The use according to claim 1 or 2, wherein the peach peach kernel compound extract is used in an amount of from 0.00001 to 100% by weight.
4. The use according to claim 1 or 2, wherein the peach peach kernel compound extract is used in an amount of 0.0001 to 50% by weight.
5. The use according to claim 1 or 2, wherein the peach peach kernel compound extract is used in an amount of from 0.001 to 20% by weight.
6. The use according to claim 2, wherein the cosmetic is selected from the group consisting of: facial cleansers, lotions, lotions, creams, gels, masks, shampoos, shower gels, hair conditioners.
7. The use according to claim 6 wherein the cosmetic comprises a peach peach kernel compound extract and an acceptable adjuvant.
8. The use according to claim 6, wherein the cosmetic comprises from 0.00001 to 100% by weight of a peach peach kernel compound extract.
9. The use according to claim 6, wherein the cosmetic comprises 0.0001 to 50% by weight of a peach peach kernel compound extract.
10. The use according to claim 6, wherein the cosmetic comprises 0.001 to 20% by weight of a peach peach kernel compound extract.

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