CN111494284A - Dendrobium officinale extract processed by special method and application thereof in skin care products - Google Patents
Dendrobium officinale extract processed by special method and application thereof in skin care products Download PDFInfo
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- CN111494284A CN111494284A CN202010484013.6A CN202010484013A CN111494284A CN 111494284 A CN111494284 A CN 111494284A CN 202010484013 A CN202010484013 A CN 202010484013A CN 111494284 A CN111494284 A CN 111494284A
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- dendrobium officinale
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- powder
- stems
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- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 77
- 239000000284 extract Substances 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims description 33
- 102000003425 Tyrosinase Human genes 0.000 claims abstract description 36
- 108060008724 Tyrosinase Proteins 0.000 claims abstract description 36
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 23
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 23
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 20
- 238000002360 preparation method Methods 0.000 claims abstract description 15
- 238000012545 processing Methods 0.000 claims abstract description 5
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 40
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 30
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 29
- 239000000843 powder Substances 0.000 claims description 26
- 238000001035 drying Methods 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 25
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
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- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 5
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- 238000002386 leaching Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims 2
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- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 14
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- 239000002537 cosmetic Substances 0.000 description 7
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- 229940033280 alpha-arbutin Drugs 0.000 description 6
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- 239000012071 phase Substances 0.000 description 6
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- AIGAZQPHXLWMOJ-UHFFFAOYSA-N Tanshinone I Chemical compound C1=CC2=C(C)C=CC=C2C(C(=O)C2=O)=C1C1=C2C(C)=CO1 AIGAZQPHXLWMOJ-UHFFFAOYSA-N 0.000 description 4
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 4
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- 230000002087 whitening effect Effects 0.000 description 4
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 3
- 208000003351 Melanosis Diseases 0.000 description 3
- 229920002125 Sokalan® Polymers 0.000 description 3
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 3
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- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
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- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 2
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- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 description 2
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- KIUKXJAPPMFGSW-YXBJCWEESA-N (2s,4s,5r,6s)-6-[(2s,3r,5s,6r)-3-acetamido-2-[(3s,4r,5r,6r)-6-[(3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H](C(O[C@@H]3[C@@H]([C@@H](O)C(O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)C(C(O)=O)O1 KIUKXJAPPMFGSW-YXBJCWEESA-N 0.000 description 1
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 description 1
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- 102000030523 Catechol oxidase Human genes 0.000 description 1
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- 108010035532 Collagen Proteins 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- -1 DPPH free radical Chemical class 0.000 description 1
- AHMIDUVKSGCHAU-UHFFFAOYSA-N Dopaquinone Natural products OC(=O)C(N)CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-UHFFFAOYSA-N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
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- 241000208680 Hamamelis mollis Species 0.000 description 1
- AHMIDUVKSGCHAU-LURJTMIESA-N L-dopaquinone Chemical compound [O-]C(=O)[C@@H]([NH3+])CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-LURJTMIESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 101000773110 Pelophylax lessonae Tyrosinase Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 206010051246 Photodermatosis Diseases 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
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- 229920001436 collagen Polymers 0.000 description 1
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- XXJWXESWEXIICW-UHFFFAOYSA-N diethylene glycol monoethyl ether Chemical compound CCOCCOCCO XXJWXESWEXIICW-UHFFFAOYSA-N 0.000 description 1
- VJNCICVKUHKIIV-UHFFFAOYSA-N dopachrome Chemical compound O=C1C(=O)C=C2NC(C(=O)O)CC2=C1 VJNCICVKUHKIIV-UHFFFAOYSA-N 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
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- 229920002549 elastin Polymers 0.000 description 1
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- 239000012467 final product Substances 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 208000000069 hyperpigmentation Diseases 0.000 description 1
- 230000003810 hyperpigmentation Effects 0.000 description 1
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- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000008099 melanin synthesis Effects 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000007922 nasal spray Substances 0.000 description 1
- 229940097496 nasal spray Drugs 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
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- 229960005323 phenoxyethanol Drugs 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000008845 photoaging Effects 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920000166 polytrimethylene carbonate Polymers 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007665 sagging Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
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- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a dendrobium officinale extract with antioxidant and tyrosinase inhibitory activity, and a preparation method and application thereof. The result shows that the special processing treatment can obviously improve the oxidation resistance and tyrosinase inhibition capability of different components, and the obtained dendrobium officinale extract can be applied to skin care product compositions.
Description
Technical Field
The invention relates to the field of natural products and skin care products, and mainly relates to a dendrobium officinale extract with antioxidant and tyrosinase inhibitory activities, which is processed by a special method, a preparation method thereof and application thereof in skin care products.
Background
The clinical process of wrinkles, freckles, melanin hyperpigmentation, dullness and elasticity reduction caused by uv irradiation is called photoaging, and mainly because excessive uv irradiation causes excessive free radicals or Reactive Oxygen Species (ROS) to be generated in vivo, thereby causing oxidative damage to the body. In addition, ROS also activate the activation of upstream kinases to activate mitogen-activated protein kinase (MAPK), resulting in degradation of collagen and elastin, and the skin may develop symptoms of aging such as sagging, wrinkles, dryness, etc. Therefore, the development of skin care products containing active ingredients for scavenging free radicals has an important effect on skin aging resistance.
Tyrosinase, also known as polyphenol oxidase, is a key enzyme in the synthesis of melanin in the human body. Tyrosine generates dopa, dopaquinone and dopachrome in sequence under the catalytic action of tyrosinase, and the final product is melanin. The skin color of human depends on the content and distribution range of melanin in human body, and when the melanin level is abnormal, the skin color is often accompanied by the pathological changes such as chloasma, freckle and even melanoma. Scholars at home and abroad control the activity level of tyrosinase by adopting an effective tyrosinase inhibitor, so that the generation of melanin is influenced, the synthesis amount is reduced, and the whitening effect is achieved.
At present, tyrosinase inhibitors are derived from fermentation, artificial synthesis, natural plants and the like, and are widely applied to products in the field of whitening cosmetics, common inhibitors comprise kojic acid, arbutin, vitamin C, hydroquinone and the like, but the inhibitors mostly have certain side effects or stability problems, the inhibitors are not satisfactory in product application, such as low stability of kojic acid and carcinogenic risk after long-term use, and hydroquinone is listed as a forbidden additive by multiple countries. Therefore, the search for safe and effective tyrosinase inhibitors is the subject of intense research in the field of cosmetic applications and is an urgent need in the market.
A large number of research results and clinical experiments prove that the traditional Chinese medicine and the extract thereof have the unique advantages of mild property, good tolerance, small toxic and side effect, lasting effect and the like, the plant-derived antioxidant and tyrosinase inhibitor meet the safety requirements of consumers, and the dendrobium officinale extract has good antioxidant and tyrosinase inhibitory activity simultaneously and is suitable to be used as a new safe and effective skin care product additive.
Disclosure of Invention
The invention aims to provide a dendrobium officinale extract with antioxidant and tyrosinase inhibitory activities, which is processed by a special method, a preparation method thereof and application thereof in skin care products.
The object of the present invention is achieved by the following technical means. The dendrobium officinale extract with the antioxidant and tyrosinase inhibitory activities, which is processed by the special method, is prepared by processing and crushing fresh dendrobium officinale by the special method, then performing cold-soaking extraction by ethanol, then performing extraction by petroleum ether, ethyl acetate and n-butanol, and finally performing reduced pressure concentration and drying to obtain the dendrobium officinale extract with the antioxidant and tyrosinase inhibitory activities.
The dendrobium officinale extract with antioxidant and tyrosinase inhibitory activities, which is prepared by the special method, and the preparation method thereof mainly comprise the following specific steps:
(1) selecting fresh stems of dendrobium officinale, removing fibrous roots, leaves and impurities, keeping stems, cleaning, and tedding for 1-2 days;
(2) preheating a medicine frying pot, adjusting the temperature to 90-120 ℃, putting the spread and dried stems into the pot, stir-frying until the leaf sheaths are opened and the stems are soft, taking out the stems, and continuously stir-frying in the stir-frying process to avoid scorching;
(3) twisting and twisting the fried soft stem strips of the dendrobium, and then placing the stem strips in a drying air box at 50 ℃ for dehydration until the stem strips are golden yellow and slightly dark, and the water content is reduced to below 8 percent, thus obtaining the processed dendrobium officinale product.
(4) Pulverizing the processed herba Dendrobii to obtain herba Dendrobii powder; mixing Dendrobium officinale powder with 70-90% ethanol at a weight ratio of 1:1-1:3, cold soaking and extracting for 1-3 times, each time for 1-2 hr, mixing to obtain extractive solution, concentrating under reduced pressure, and drying to obtain herba Dendrobii total extract.
Preferably, the obtained extracting solution is extracted by petroleum ether, then extracted by ethyl acetate, and the obtained ethyl acetate phase is subjected to reduced pressure concentration and drying to obtain the dendrobium officinale extract processed by the special method. After the steps are carried out, the antioxidant activity and the tyrosinase inhibition activity of the obtained extract are obviously enhanced.
Preferably, the dendrobium officinale processed according to the special method is extracted with 70-90% ethanol for three times according to the mass ratio of 1:2, and the extraction time is 40-100min each time.
Most preferably, the dendrobium officinale processed according to the special method is extracted with 70-90% ethanol for three times in a mass ratio of 1:3, 1:2 and 1:1, wherein the extraction time is 60-90min each time.
Preferably, the extract is subjected to reduced pressure concentration at 50-60 ℃ and drying to obtain the dendrobium officinale total extract.
Preferably, the ethyl acetate extraction step is carried out for 2 to 5 times according to the volume ratio of 0.5 to 2:1, and each time of extraction is 30 to 90 min.
Preferably, the ethyl acetate extraction step is carried out according to the volume ratio of 0.8-1.2:1, and the extraction is carried out for 3-5 times, and each time, the extraction lasts for 40-60 min.
Most preferably, the ethyl acetate extraction step is performed for 3 times according to the volume ratio of 0.9:1, and each time of extraction is 50-60 min.
The invention also provides a dendrobium officinale extract with antioxidant and tyrosinase inhibitory activity, which is prepared by a special method, and the dendrobium officinale extract is prepared by the following method:
s1, selecting fresh stems of dendrobium officinale which do not bloom, removing fibrous roots, leaves and impurities, keeping stems, cleaning, and tedding for 1-2 days;
s2, preheating a medicine frying pan, adjusting the temperature to 90-120 ℃, putting the tedded stems into the pan, stir-frying until the leaf sheaths are open and the stems are soft, taking out the stems, and continuously stir-frying in the stir-frying process to avoid scorching;
s3, twisting and twisting the fried and soft stem strips of dendrobium, and then dehydrating in a drying air box at 50 ℃ until the stem strips are golden yellow and slightly dark, and the water content is reduced to below 8 percent to obtain the processed dendrobium officinale product;
s4, crushing the processed dendrobium officinale to obtain dendrobium officinale powder, mixing the dendrobium officinale powder with 70-90% ethanol according to the weight ratio of 1:1-1:3, carrying out cold-leaching extraction for 1-3 times for 1-2 hours each time, mixing to obtain an extracting solution, concentrating the extracting solution under reduced pressure, drying and crushing to obtain a dendrobium officinale crude extract powder MOE;
s5, mixing the extracting solution obtained in the step S4 and petroleum ether according to the weight ratio of 1:2, extracting for 3 times, each time for 50min, obtaining a petroleum ether layer at the uppermost layer, a middle layer and a water layer at the lowermost layer, removing the middle layer, collecting the petroleum ether layer, concentrating, drying and crushing to obtain powder MPE;
s6, mixing the water layer and the ethyl acetate in the step S5 according to the weight ratio of 1:2, extracting for 3 times, 50min each time, obtaining an upper ethyl acetate layer and a lower water layer, collecting the ethyl acetate layer, concentrating, drying and crushing to obtain powder MEA;
and S7, mixing the water layer and n-butanol obtained in the step S6 according to the weight ratio of 1:2, extracting for 3 times for 50min each time to obtain an upper n-butanol layer and a lower water layer, collecting the n-butanol layer, concentrating, drying and crushing to obtain powder MBA.
The invention also provides a skin care product which comprises the dendrobium officinale extract and at least one or more common carriers for the skin care product.
The invention also provides application of the dendrobium officinale extract in preparation of skin care products.
And the application of the dendrobium officinale extract in preparing products with antioxidant, anti-aging and tyrosinase inhibitory activities.
In the application, the skin care products and products contain the dendrobium officinale extract with the mass fraction of 0.1-30%.
Preferably, the skin care product can be cream, ointment, lotion, suspension, soap, and foundation.
The invention has the beneficial effects that: the dendrobium officinale extract obtained by the preparation method disclosed by the invention has no toxic or side effect, not only has good antioxidant activity, but also has an excellent tyrosinase inhibition effect, and can be used for achieving the effects of whitening and moisturizing the skin by removing epidermal cell free radicals and inhibiting the activity of skin tyrosinase to influence the generation of melanin. The dendrobium officinale is a traditional rare medicinal material, and the skin care product applying the extract and the technology has no toxic or side effect, so that a development and utilization approach of the dendrobium officinale is provided.
The specific implementation mode is as follows:
in order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments, but the invention is not limited by the specific embodiments. Simple modifications of the invention in accordance with its spirit fall within the scope of the invention.
Example 1
Preparing the dendrobium officinale extract with antioxidant and tyrosinase inhibitory activities, which is processed by a special method.
S1, selecting fresh stems of dendrobium officinale which do not bloom, removing fibrous roots, leaves and impurities, keeping stems, cleaning, and tedding for 1-2 days;
s2, preheating a medicine frying pan, adjusting the temperature to 90-120 ℃, putting the tedded stems into the pan, stir-frying until the leaf sheaths are open and the stems are soft, taking out the stems, and continuously stir-frying in the stir-frying process to avoid scorching;
s3, twisting and twisting the fried and soft stem strips of dendrobium, and then dehydrating in a drying air box at 50 ℃ until the stem strips are golden yellow and slightly dark, and the water content is reduced to below 8 percent, thus obtaining the dendrobium officinale processed product.
S4, pulverizing the processed herba Dendrobii to obtain herba Dendrobii powder; mixing Dendrobium officinale powder with 70-90% ethanol according to a weight ratio of 1:1-1:3, cold soaking and extracting for 1-3 times, each time extracting for 1-2 hours, mixing to obtain an extracting solution, concentrating under reduced pressure, drying and pulverizing to obtain Dendrobium officinale crude extract powder MOE.
And S5, mixing the extracting solution obtained in the step S4 and petroleum ether according to the weight ratio of 1:2, extracting for 3 times, each time for 50min, obtaining a petroleum ether layer at the uppermost layer, a middle layer and a water layer at the lowermost layer, removing the middle layer, collecting the petroleum ether layer, concentrating, drying and crushing to obtain powder MPE.
And S6, mixing the water layer and the ethyl acetate in the step S5 according to the weight ratio of 1:2, extracting for 3 times and 50min each time to obtain an upper ethyl acetate layer and a lower water layer, collecting the ethyl acetate layer, concentrating, drying and crushing to obtain the powder MEA.
And S7, mixing the water layer and n-butanol obtained in the step S6 according to the weight ratio of 1:2, extracting for 3 times for 50min each time to obtain an upper n-butanol layer and a lower water layer, collecting the n-butanol layer, concentrating, drying and crushing to obtain powder MBA.
Example 2
The embodiment mainly comprises the step of carrying out DPPH free radical scavenging activity experimental test and tyrosinase inhibition activity test on the dendrobium officinale active extract prepared in the embodiment 1. The method comprises the following specific steps:
(1) the required drugs DPPH (1, 1-diphenyl-2-trinitrophenylhydrazine), water-soluble vitamin E (Trolox), α -arbutin (α -arbutin), mushroom tyrosinase and levodopa (L-Dopa) were purchased from Sigma.
(2) DPPH free radical scavenging activity assay:
the experimental method comprises the following steps: on a 96-well plate, a sample to be detected and DPPH (final concentration is 100 mu M) are mixed and reacted, 3 repeated wells are set, meanwhile, a blank control without medicine and a Trolox positive control are set, after reaction for 1h at 30 ℃, an enzyme-labeling instrument is used for measuring an OD value, and the detection wavelength is 515 nm.
The percent oxidation resistance (1-experimental well OD515 nm/blank well OD515nm) × 100%
(3) Tyrosinase inhibitory activity:
the experimental method comprises the steps of mixing a sample to be detected (the sample to be detected and the positive control α -arbutin are both 100 mu g/m L) with levodopa (L-Dopa, the final concentration is 1.25mM), adding tyrosinase (the final concentration is 25U/m L) to start reaction, setting 3 repeated holes, setting a blank control without medicines and a α -arbutin (α -arbutin) positive control, reacting for 5min at room temperature, measuring an OD value by using an enzyme labeling instrument, and calculating the tyrosinase activity inhibition rate when the detection wavelength is 490 nm.
Tyrosinase activity inhibition (%) of × 100% (1-sample OD490 nm/experimental control OD490nm) ×%
(4) And (3) testing results:
TABLE 1 DPPH clearance and tyrosinase inhibition test results of Dendrobium officinale active extract processed by special method
| Test sample | Concentration (μ g/m L) | DPPH clearance (%) | Tyrosinase inhibition (%) |
| MOE | 100 | 20.25 | 17,64 |
| MPE | 100 | 11,16 | 12.37 |
| MEA | 100 | 48.14 | 50.50 |
| MBA | 100 | 38.92 | 41.81 |
| Trolox (positive control) | 25 | 95.07 | —— |
| α -arbutin (Positive control) | 100 | —— | 22.16 |
From the above tests and test data it can be seen that:
when the test concentration of the active extract MEA (ethyl acetate extract phase) of Dendrobium officinale prepared in the embodiment 1 is 100ug/m L, the DPPH free radical clearance rate reaches 48%, and the tyrosinase inhibitory activity reaches 50%, so that the active extract of Dendrobium officinale prepared in the embodiment of the invention has good antioxidant activity and strong melanin generation inhibitory activity.
Examples 3 and 4
And (3) preparing the dendrobium officinale skin care essence.
| Components | Example 3 | Example 4 |
| Dendrobium officinale extract | 2％ | 5％ |
| Hyaluronic acid sodium salt | 1％ | 1％ |
| 1, 3-propanediol | 5％ | 5％ |
| Ethoxy diglycol | 1％ | 1％ |
| Polyacrylate Cross-Linked Polymer-6 | 0.5％ | 0.5％ |
| PPG-26-Butaneth-26 | 0.3％ | 0.3％ |
| PEG-40 hydrogenated Castor oil | 0.2％ | 0.2％ |
| Lactic acid | 1％ | 1％ |
| EDTA disodium salt | 0.05％ | 0.05％ |
| Phenoxyethanol | 0.4％ | 0.4％ |
| Chlorophytidine ester | 0.1％ | 0.1％ |
| Deionized water | To 100 percent | To 100 percent |
The essences of examples 3 and 4 were prepared according to the following steps:
(1) preparing an oil phase: adding oil, fat, emulsifier and other oil soluble components into a dissolving pot, heating to 70-75 deg.C under stirring, and melting or dissolving uniformly;
(2) preparation of an aqueous phase: adding deionized water and water soluble components into dissolving pan, heating to 90-100 deg.C under stirring, maintaining for 25min for sterilization, and cooling to 70-80 deg.C;
(3) emulsification and cooling: adding the oil phase and the water phase into an emulsifying pot through a filter, stirring and emulsifying at 70-80 deg.C for 5-10min, naturally cooling the emulsified system to room temperature, adding heat-sensitive components, and stirring thoroughly.
Example 5:
nasal spray: the dendrobium officinale active extract prepared in the example 1 accounts for 0.2 percent
The preparation method comprises the following steps: adding one component into double distilled water with stirring until completely deeply decomposing, and adding the other component. After adding distilled water to 2ml, the solution was filtered on a sterile filter, bottled and partitioned according to the appropriate dose.
Example 6:
formula (W%) of whitening cream containing the dendrobium officinale active extract prepared in example 1:
deionized water to 100
The cosmetic with the formula is prepared by a conventional cosmetic preparation method.
Example 7:
emulsion formula (W%) containing the dendrobium officinale active extract prepared in example 1:
the cosmetic with the formula is prepared by a conventional cosmetic preparation method.
Example 8:
cosmetic water:
water phase: 0.35 g of sodium polyacrylate, 4 g of glycerol, 2.5 g of 1, 3-butanediol, 2.5 g of witch hazel extract, 50.5 g of vitamin B, 0.5 g of arbutin and EDTA-Na 250 mg; deionized water, appropriate amount, add to 100 ml. The preparation method comprises the following steps: 0.3 g of sodium polyacrylate is dissolved in 70ml of water, stirred and fully swelled. Slowly adding the rest components in the prescription, and stirring continuously; then 0.2% of the dendrobium officinale active extract prepared in example 1 is added, and stirred to be uniform. Adding deionized water to 100ml, and packaging.
Example 9:
repairing cream:
prescription: 6 g of glycerol, 1.5 g of carbomer, 1.5 g of triethanolamine, 6 g of propylene glycol, 0.2 g of ethyl hydroxybenzoate, 1.2 g of tanshinone extract, active extract of dendrobium officinale and a proper amount of deionized water, and the total weight is 100 g. The preparation method comprises the following steps: adding carbomer into deionized water, stirring, standing overnight to swell completely, adding glycerol, and adjusting pH with triethylamine to increase gel matrix viscosity. And uniformly mixing the tanshinone extract, the propylene glycol and the deionized water according to the prescription amount. Adding carbomer gel, adding 0.5% herba Dendrobii active extract and ethyl hydroxybenzoate, mixing, stirring, adding distilled water, and grinding.
The foregoing description should be understood as merely illustrative of the present invention. Various alternatives and modifications can be devised by those skilled in the art without departing from the invention. Accordingly, it is intended that the present invention embrace all such alternatives, modifications and variations as fall within the scope of the appended claims and the description.
Claims (10)
1. A preparation method of a dendrobium officinale extract with antioxidant and tyrosinase inhibitory activities is characterized by comprising the following steps: processing Dendrobium officinale by a special method, and separating to obtain Dendrobium officinale extracts with different components and enhanced antioxidant and tyrosinase inhibitory activities.
2. The method of claim 1, wherein the extract of Dendrobium officinale having antioxidant and tyrosinase inhibitory activities comprises: the fresh stem of dendrobium officinale is a 2-3-year fresh stem of dendrobium officinale with membranous leaf sheaths growing on the stem; the processing temperature of the special method is 90-120 ℃; the dendrobium officinale processed by the special method is extracted by a solvent, and extracts of different antioxidant and tyrosinase inhibitory active components are obtained according to the polarity of the solvent.
3. The method for preparing the dendrobium officinale extract with antioxidant and tyrosinase inhibitory activities according to claim 2, wherein the extract comprises the following components: the dendrobium officinale processed by the special method is extracted and separated by 90% ethanol, petroleum ether, ethyl acetate and n-butyl alcohol in sequence to obtain the dendrobium officinale extract with different components and enhanced antioxidant and tyrosinase inhibitory activity.
4. The special processing method of dendrobium officinale according to claim 1, which is characterized in that: the method for processing the dendrobium officinale by a special method comprises the following steps:
(1) selecting fresh stems of dendrobium officinale, removing fibrous roots, leaves and impurities, keeping stems, cleaning, and tedding for 1-2 days;
(2) preheating a medicine frying pot, adjusting the temperature to 90-120 ℃, putting the spread and dried stems into the pot, stir-frying until the leaf sheaths are opened and the stems are soft, taking out the stems, and continuously stir-frying in the stir-frying process to avoid scorching;
(3) twisting and twisting the fried soft stem strips of the dendrobium, and then placing the stem strips in a drying air box at 50 ℃ for dehydration until the stem strips are golden yellow and slightly dark, and the water content is reduced to below 8 percent, thus obtaining the processed dendrobium officinale product.
5. The method for preparing the dendrobium officinale extract with antioxidant and tyrosinase inhibitory activities according to claim 1 or 4, wherein the extract comprises the following components: the method comprises the following steps:
(1) pulverizing processed or unprocessed herba Dendrobii to obtain herba Dendrobii powder; mixing Dendrobium officinale powder with 70-90% ethanol according to a weight ratio of 1:1-1:3, cold-soaking and extracting for 1-3 times, each time extracting for 1-2 hours, mixing to obtain an extracting solution, concentrating, drying and crushing to obtain Dendrobium officinale crude extract powder MOE;
(2) mixing the extracting solution in the step (1) with petroleum ether according to the weight ratio of 1:2-2:1, standing and extracting to obtain a petroleum ether layer at the uppermost layer, a middle layer and a water layer at the lowermost layer, removing the middle layer, collecting the petroleum ether layer, concentrating, drying and crushing to obtain MPE powder;
(3) mixing the water layer obtained in the step (2) with ethyl acetate according to the weight ratio of 1:2-2:1, standing and extracting to obtain an upper ethyl acetate layer and a lower water layer, collecting the ethyl acetate layer, concentrating, drying and crushing to obtain powder MEA;
(4) and (3) mixing the water layer in the step (3) with n-butanol according to the weight ratio of 1:2-2:1, standing and extracting to obtain an upper n-butanol layer and a lower water layer, collecting the n-butanol layer, concentrating, drying and crushing to obtain powder MBA.
6. The dendrobium officinale extract with antioxidant and tyrosinase inhibitory activity, which is processed by a special method, is characterized by being prepared by the following method:
s1, selecting fresh stems of dendrobium officinale which do not bloom, removing fibrous roots, leaves and impurities, keeping stems, cleaning, and tedding for 1-2 days;
s2, preheating a medicine frying pan, adjusting the temperature to 90-120 ℃, putting the tedded stems into the pan, stir-frying until the leaf sheaths are open and the stems are soft, taking out the stems, and continuously stir-frying in the stir-frying process to avoid scorching;
s3, twisting and twisting the fried and soft stem strips of dendrobium, and then dehydrating in a drying air box at 50 ℃ until the stem strips are golden yellow and slightly dark, and the water content is reduced to below 8 percent to obtain the processed dendrobium officinale product;
s4, crushing the processed dendrobium officinale to obtain dendrobium officinale powder, mixing the dendrobium officinale powder with 70-90% ethanol according to the weight ratio of 1:1-1:3, carrying out cold-leaching extraction for 1-3 times for 1-2 hours each time, mixing to obtain an extracting solution, concentrating the extracting solution under reduced pressure, drying and crushing to obtain a dendrobium officinale crude extract powder MOE;
s5, mixing the extracting solution obtained in the step S4 and petroleum ether according to the weight ratio of 1:2, extracting for 3 times, each time for 50min, obtaining a petroleum ether layer at the uppermost layer, a middle layer and a water layer at the lowermost layer, removing the middle layer, collecting the petroleum ether layer, concentrating, drying and crushing to obtain powder MPE;
s6, mixing the water layer and the ethyl acetate in the step S5 according to the weight ratio of 1:2, extracting for 3 times, 50min each time, obtaining an upper ethyl acetate layer and a lower water layer, collecting the ethyl acetate layer, concentrating, drying and crushing to obtain powder MEA;
and S7, mixing the water layer and n-butanol obtained in the step S6 according to the weight ratio of 1:2, extracting for 3 times for 50min each time to obtain an upper n-butanol layer and a lower water layer, collecting the n-butanol layer, concentrating, drying and crushing to obtain powder MBA.
7. The skin care product comprises the dendrobium officinale extract as defined in claim 6 and at least one or more common carriers for skin care products.
8. The use of the dendrobium officinale extract of claim 6 in the preparation of skin care products.
9. The use of the Dendrobium officinale extract of claim 6 in the preparation of products with antioxidant, anti-aging and tyrosinase inhibitory activity.
10. The use according to claim 8 or 9, characterized in that the skin care products and products contain dendrobium officinale extract in a mass fraction of 0.1-30%.
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Application publication date: 20200807 |