JP2010083786A - Anti-aging agent and skin cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an anti-aging agent containing a natural extract and having excellent safety, a laminin 5 production promoter, an agent for damage recovery action by ultraviolet (UV-B) irradiation, an I type collagen production promoter, a profilaggrin production promoter, a filaggrin production promoter or a skin cosmetic. <P>SOLUTION: The anti-aging agent includes an extract from Caragana korshinskii and/or Camellia oleifera as an active ingredient. The laminin 5 production promoter, or the agent for damage recovery action by ultraviolet (UV-B) irradiation includes an extract from Caragana korshinskii as an active ingredient. The I type collagen production promoter, the profilaggrin production promoter or the filaggrin production promoter includes an extract from Camellia oleifera as an active ingredient. The skin cosmetic includes an extract from Caragana korshinskii and/or Camellia oleifera. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to an anti-aging agent and a skin cosmetic containing a natural extract.

  The skin is composed of the stratum corneum, epidermis, basement membrane and dermis. The basement membrane is present at the boundary between the epidermis and the dermis and plays an important role not only in connecting the epidermis and the dermis but also in maintaining the skin function (see Non-Patent Document 1). The main skeleton of the basement membrane has a network structure composed of type IV collagen. Various glycoproteins mainly composed of laminin 5 are present at the boundary between the basement membrane and the epidermis and connect the basement membrane and the epidermis. Such laminin 5 is produced from epidermal keratinocytes present in the epidermis. Is done. In young skin, the basement membrane works to maintain the homeostatic interaction of the epidermis and dermis, ensuring moisture retention, flexibility, elasticity, and the like. Maintained.

  However, laminin 5 which is a main component of the basement membrane degrades and deteriorates when it is affected by certain external factors such as ultraviolet irradiation, drastic air drying, excessive skin washing, etc., or when aging progresses. And the basement membrane structure is destroyed (see Non-Patent Document 2). As a result, the skin's moisturizing function and elasticity are lowered, and the keratin begins to exfoliate abnormally, so the skin loses its tension and gloss, and exhibits aging symptoms such as roughness and wrinkles. As described above, changes accompanying skin aging, that is, wrinkles, dullness, disappearance of texture, decrease in elasticity, etc. are associated with a decrease in basement membrane components and a change in basement membrane structure, and production of laminin 5 It is considered that aging symptoms of the skin can be prevented and improved by promoting the above.

  Laminin consists of various combinations of α chain, β chain, and γ chain, and 15 types (laminin 1 to laminin 15) are currently known. These α chain, β chain, and γ chain form a coiled triple chain structure at a site called a long arm, thereby forming a huge laminin molecule. Among them, laminin 5 (α3β3γ2) is abundantly present in the basement membrane of epithelial tissues such as skin, digestive organs, kidneys and lungs. In genetic diseases caused by congenital abnormalities of the genes encoding each chain of laminin 5 (lethal congenital epidermolysis bullosa, Herlitz junctional epidermolysis bullosa), the epidermis of the whole body may show fatal symptoms Are known. Laminin 5 is known to strongly adhere cells (high cell adhesion activity) and strongly promote cell motility (high cell motility activity) compared to other extracellular matrix molecules (non-cell motility activity). (See Patent Document 3).

  Thus, since laminin 5 has a high cell motility activity, it is known that laminin 5 promotes cell migration in damaged skin and promotes wound healing (see Patent Document 1). That is, promoting the production of laminin 5 is important in promoting healing of skin damage that destroys the structure of the basement membrane.

  Conventionally, a skin external preparation containing laminin 5 (see Patent Document 2) has been known, and soy extract (see Patent Document 3), phenylpropanoids (Patent Document 3) have an action of promoting laminin 5 production. Literature 4), pantothenic acid (see Patent Literature 5), lysophosphatidylcholine or lysophosphatidic acid (see Patent Literature 6), coenzyme Q10 (see Patent Literature 7) and the like are known.

  When the skin is exposed to excessive UV rays, the skin cells produce active oxygen, cytokines, etc. due to the stimulation of the UV rays, and act on the cells themselves and surrounding cells, causing an acute inflammatory reaction such as sunburn, that is, inflammation such as blisters on the skin Is formed, which in turn causes adverse effects such as pigmentation. Moreover, it is known that the active oxygen generated at this time causes DNA damage. In cells in which DNA has been damaged, a tumor suppressor gene protein called p53 is expressed in the cell, and this protein is known to induce DNA repair, cell cycle arrest, and apoptosis depending on the degree of DNA damage. Yes. Increased apoptosis in the skin may lead to a decrease in tissue regeneration ability and may promote skin aging. Therefore, if the damage caused by ultraviolet irradiation can be prevented / improved, it is considered that the skin moisturizing ability, elasticity, barrier function and other skin functions can be maintained.

  Further, it is known that chronic exposure to ultraviolet rays promotes skin aging (photoaging) and contributes to skin cancer as well as spots and wrinkles. As a result, it is considered that skin aging, wrinkle formation, and the like occur, and the skin function decreases. Therefore, it is considered that prevention / improvement of skin inflammatory reaction induced by ultraviolet rays and subsequent skin function disorder also lead to prevention / improvement of skin aging and cancer.

  Conventionally, sunscreen products containing ultraviolet absorbers such as benzophenone derivatives and inorganic ultraviolet scattering agents such as titanium oxide and zinc oxide have been used as a method for preventing damage caused by ultraviolet irradiation (see Patent Documents 8 to 10). . However, although these sunscreen products have a high UV protection effect, they are satisfied as a continuous preventive effect due to problems in use and physical durability such as friction resistance and sweat resistance. However, it is also a problem in terms of safety, such as causing inflammation with an ultraviolet absorber. In addition, there is a demand for the development of a preparation that can prevent or ameliorate inflammation caused by exposure to ultraviolet rays and subsequent damage. For example, an extract from licorice root (see Patent Document 11) is known as having such a damage recovery effect by ultraviolet (UV-B) irradiation.

  The epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin, hyaluronic acid, and collagen that are outside these cells and support the skin structure. In young skin, the proliferation of fibroblasts is active, and the interaction between skin tissues such as fibroblasts and collagen keeps homeostasis, ensuring moisture retention, flexibility, elasticity, etc. It is maintained in a fresh state with its tension and gloss.

  However, when there is an influence of certain external factors such as irradiation of ultraviolet rays, significant drying of air, excessive skin washing, etc., or when aging progresses, collagen (type I collagen), which is a major component of the extracellular matrix. Etc.) and the elasticity decreases due to crosslinking. As a result, the skin retains its moisturizing function and elasticity, and the keratin begins to exfoliate abnormally, so that the skin loses its tension and gloss and exhibits aging phenomena such as rough skin and wrinkles. As described above, changes accompanying skin aging, that is, wrinkles, dullness, disappearance of texture, decrease in elasticity, and the like involve reduction / denaturation of dermal matrix components such as collagen (type I collagen, etc.). . Therefore, it is considered that aging of skin can be prevented and / or improved by promoting the production of collagen (such as type I collagen) in dermal fibroblasts.

  Conventionally, as a herbal medicine having a collagen production promoting action, a quintuce extract (see Patent Document 12) is known.

  An amino acid which is a main component of natural moisturizing factors (NMF) is produced by degrading filaggrin derived from keratohyalin granules in the stratum corneum. This filaggrin is expressed as profilagrin in epidermal keratinocytes present in the granule layer immediately below the stratum corneum. After that, it is immediately phosphorylated, accumulated in keratohyalin granules, decomposed to filaggrin through dephosphorylation and hydrolysis, transferred to the stratum corneum, increases the aggregation efficiency of keratin filaments and participates in the internal construction of keratinocytes It is known (see Non-Patent Document 1).

  In recent years, it has been known that this filaggrin is very important and indispensable for moisture retention of the skin, and that the synthetic power of filaggrin is reduced by conditions such as drying, and the amount of amino acids in the stratum corneum is reduced ( Non-patent document 2). It is known that when the amount of amino acid in the stratum corneum decreases and the water content of the stratum corneum decreases, the stratum corneum becomes hard, causing cracks in the stratum corneum and causing skin wrinkles and the like.

  Therefore, in the epidermal keratinocytes, by promoting the production of profilagrin, the production of filaggrin and thereby increasing the amount of amino acids in the stratum corneum can essentially improve the water environment of the stratum corneum. It is expected that wrinkles can be prevented.

  As what has such profilaggulin production promotion effect and filaggrin production promotion effect, for example, liquorice (refer patent document 13), liquiritin known as flavanone glycoside contained in a natural plant (refer patent document 14) For example, plant extracts or yeast extracts belonging to the genus Citrus (see Patent Document 15) are known as those having profilaggrin and filaggrin protein production promoting action.

In addition, it is known that a spear extract has an antiallergic action, a leucine dehydrogenase inhibitory action, etc. (refer patent documents 16 and 17), and an oil tea extract is known to have an antibacterial action. However, it is not known at all that these extracts have an anti-aging effect (see Patent Document 18).
JP 2006-63033 A Japanese Patent Laid-Open No. 10-147515 JP 2004-217618 A JP 2007-77169 A JP 2005-179243 A JP 2000-226308 A JP 2007-63160 A JP-A-6-305949 Japanese Patent Laid-Open No. 7-145029 JP-A-8-259419 JP 2004-250368 A JP 2002-226323 A JP 2002-363054 A JP 2003-146886 A JP 2001-261568 A JP-A-11-180885 JP 2002-87973 A JP 2008-69138 A "Fragrance Journal Extra Number", 2000, Vol.17, p.14-19 "Arch. Dermatol. Res.", 1996, Vol.288, p.442-446

  The present invention finds an anti-aging effect from natural products with high safety, an anti-aging agent containing the same as an active ingredient, an agent for promoting laminin 5 production, and an agent for recovering damage by ultraviolet (UV-B) irradiation An object of the present invention is to provide a type I collagen production promoter, a profilagrin production promoter, a filaggrin production promoter, and a skin cosmetic.

  In order to solve the above-mentioned problems, the anti-aging agent of the present invention is characterized by containing an extract from bonito and / or oil tea as an active ingredient.

  Moreover, the laminin 5 production promoter of this invention and the damage recovery agent by ultraviolet-ray (UV-B) irradiation contain the extract from a shoot as an active ingredient, The type I collagen production promotion of this invention is characterized by the above-mentioned. The agent, the profilagrin production promoter and the filaggrin production promoter contain an extract from oil tea as an active ingredient.

  Furthermore, the skin cosmetic of the present invention is characterized by blending an extract from straw and / or oil tea.

  According to the present invention, an extract from natural candy and / or oil tea is contained as an active ingredient, and an anti-aging agent excellent in safety, a laminin 5 production promoter, and ultraviolet (UV-B) irradiation. A damage recovery agent, a type I collagen production promoter, a profilagrin production promoter, a filaggrin production promoter, and a skin cosmetic can be provided.

[Anti-aging agent, laminin 5 production promoter, damage recovery agent by ultraviolet (UV-B) irradiation, type I collagen production promoter, profilaggrin production promoter, filaggrin production promoter]
The present invention will be described below.
The anti-aging agent of the present invention contains an extract from candy and / or oil tea as an active ingredient. Moreover, the laminin 5 production promoter of this invention and the damage recovery agent by the ultraviolet-ray (UV-B) irradiation contain the extract from a candy as an active ingredient. Furthermore, the type I collagen production promoter, profilagrin production promoter, and filaggrin production promoter of the present invention contain an extract from oil tea as an active ingredient.

  Here, in the present invention, the “extract from cocoon and / or oil tea” includes an extract obtained from cocoon and / or oil tea as an extraction raw material, a diluted or concentrated solution of the extract, and the extract is dried. The dried product obtained in this way, or any of these crudely purified products or purified products.

The extraction raw materials used in the present invention are bonito and oil tea.
Kamajo (scientific name: Caragana chamlagu) is a deciduous shrub belonging to the genus Muresuzume, which is distributed from northern China to the Korean peninsula, etc., and can be easily obtained from these regions. Examples of the constituent parts of the ridge that can be used as the raw material for extraction include a leaf part, a flower part, a branch part, a stem part, a seed part, and a root part, and a seed part is preferable.

  Oil tea (scientific name: Camellia oleifera) is an evergreen tree belonging to the genus Camellia belonging to the south of the Yangtze River basin in China, and can be easily obtained from these areas. Examples of the constituent parts of the oil tea that can be used as the raw material for extraction include a leaf part, a flower part, a branch part, a stem part, a seed part, and a root part, and the leaf part is preferable.

  Anti-aging action, laminin 5 production promotion action, damage recovery action by ultraviolet (UV-B) irradiation, type I collagen production promotion action, profilagrin production promotion action or pro Although details of the substance having a filaggrin production promoting action are unknown, an extract having these actions can be obtained from straw and / or oil tea by an extraction method generally used for plant extraction.

  For example, after drying the plant, it is pulverized as it is or using a crusher, and subjected to extraction with an extraction solvent, whereby an extract having the above-described action can be obtained. Drying may be performed in the sun or using a commonly used dryer. Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing a pretreatment such as degreasing, extraction with a polar solvent such as cocoon strips or oil tea can be performed efficiently.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These may be used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. Is preferred.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a mixed solution of water and a lower aliphatic alcohol, it is preferable to mix 1 to 90 parts by volume of a lower aliphatic alcohol with respect to 10 parts by volume of water. When using a mixed solution, it is preferable to mix 1 to 40 parts by volume of a lower aliphatic ketone with 10 parts by volume of water, and when using a mixed solution of water and a polyhydric alcohol, water 10 It is preferable to mix 10 to 90 parts by volume of a polyhydric alcohol with respect to the volume part.

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent 5 to 15 times (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. The obtained extract is diluted, concentrated, dried, purified, etc. according to a conventional method in order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crude purified product or a purified product thereof. Processing may be performed.

  Purification can be performed by, for example, activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like. The obtained extract can be used as it is as an active ingredient of an anti-aging agent, but a concentrated solution or a dried product is easier to use.

  Extracts from cocoon strips or oil tea have a characteristic odor, and can be purified for the purpose of decolorization, deodorization, etc. within a range that does not cause a decrease in their physiological activity. Since it is not used in a large amount when it is blended, etc., there is no practical problem even if it is not purified.

  The extract from cocoon and oil tea obtained as described above has an anti-aging effect, and the extract from cocoon has a laminin 5 production promoting action and damage recovery by ultraviolet (UV-B) irradiation. The extract from oil tea has an action to promote type I collagen production, an action to promote profilagrin production, and an action to promote filaggrin production. Therefore, the extract from cocoon and / or oil tea can be used as an active ingredient of an anti-aging agent by utilizing their action, and the extract from cocoon can be used for laminin 5 by utilizing their action. It can be used as an active ingredient of a production accelerator or an agent for recovering damage caused by ultraviolet (UV-B) irradiation, and the extract from oil tea is a type I collagen production promoter, profilagrin production promoter or filaggrin production promoter. It can be used as an active ingredient. In addition, in the anti-aging agent of this invention, only one of a Koji strip extract and an oil tea extract may be used as said active ingredient, and both may be mixed and used as said active ingredient. When both are mixed and used as the active ingredient, the blending ratio may be appropriately determined according to the degree of their action.

  Here, the anti-aging effect which the extract from a cocoon has is exhibited based on the laminin 5 production promotion effect and / or the damage recovery effect by ultraviolet (UV-B) irradiation, for example. However, the anti-aging action which the extract from a cocoon has is not limited to the anti-aging action exhibited based on the said action.

  In addition, the anti-aging effect of the extract from oil tea is based on, for example, one or more actions selected from the group consisting of a type I collagen production promoting action, a profilagulin production promoting action and a filaggrin production promoting action. Demonstrated. However, the anti-aging action which the extract from oil tea has is not limited to the anti-aging action exhibited based on the said action.

  The anti-aging agent of the present invention may be composed only of an extract from straw and / or oil tea, or may be a formulation of an extract from straw and / or oil tea. Moreover, the laminin 5 production promoter of this invention or the damage recovery agent by ultraviolet-rays (UV-B) irradiation may consist only of the extract from a shoot, or the extract from a shoot is formulated. It may be converted into one. Furthermore, the type I collagen production promoter, profilagrin production promoter, or filaggrin production promoter of the present invention may consist only of an extract from oil tea, or a formulation of an extract from oil tea. It may be.

  Extracts from candy and / or oil tea can be used in the form of powder, granules, tablets, liquids, etc. according to conventional methods using pharmaceutically acceptable carriers such as dextrin and cyclodextrin and other optional auxiliaries. It can be formulated into any dosage form. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring / flavoring agent, and the like can be used. Extracts from candy and / or oil tea can be used by blending with other compositions (for example, skin cosmetics to be described later), and used as ointments, liquids for external use, patches, etc. Can do.

  In addition, the anti-aging agent of the present invention, laminin 5 production promoter, damage recovery agent by ultraviolet (UV-B) irradiation, type I collagen production promoter, profilaggrin production promoter or filaggrin production promoter are used as necessary. Formulated with other natural extracts having anti-aging effect, laminin 5 production promoting action, damage recovery effect by ultraviolet (UV-B) irradiation, type I collagen production promoting action, profilagrin production promoting action or filaggrin production promoting action And can be used as an active ingredient.

  As an administration method of the anti-aging agent, laminin 5 production promoter, damage recovery agent by ultraviolet (UV-B) irradiation, type I collagen production promoter, profilagrin production promoter or filaggrin production promoter of the present invention, Although transdermal administration and the like can be mentioned, a suitable method for the prevention / treatment or the like may be appropriately selected according to the type of disease.

  In addition, the dosage of the anti-aging agent, laminin 5 production promoter, damage recovery agent by ultraviolet (UV-B) irradiation, type I collagen production promoter, profilaggrin production promoter or filaggrin production promoter of the present invention, What is necessary is just to increase / decrease suitably according to the kind of disease, severity, an individual difference of a patient, an administration method, an administration period, etc.

  The anti-aging agent of the present invention comprises laminin 5 production promoting action and / or damage recovery action caused by ultraviolet (UV-B) irradiation, and / or type I collagen production of oil tea extract. Skin aging can be prevented, treated or ameliorated through one or more actions selected from the group consisting of a promoting action, a profilagulin production promoting action and a filaggrin production promoting action. However, the anti-aging agent of the present invention has anti-aging action, laminin 5 production promoting action, damage recovery action by ultraviolet (UV-B) irradiation, type I collagen production promoting action, and profilagrin production promoting action in addition to these uses. , It can be used for all purposes that are meaningful in exerting a filaggrin production promoting effect.

  The laminin 5 production promoter of the present invention can promote the production of laminin 5 through the laminin 5 production promoting action of the extract from the spear. Thereby, reconstruction of the basement membrane structure can be induced, and skin aging symptoms such as wrinkles, dullness, disappearance of texture, and decrease in elasticity can be prevented or improved. However, the laminin 5 production-promoting agent of the present invention can be used for all uses that are meaningful for exerting the laminin 5 production-promoting action in addition to these uses. For example, for laminin 5 deficiency (deficiency) It can be used as a preventive or therapeutic agent for diseases caused (such as epidermolysis bullosa).

  The agent for recovering damage by irradiation with ultraviolet rays (UV-B) according to the present invention is based on chronic exposure to ultraviolet rays (UV-B) through the damage recovery action by irradiation with ultraviolet rays (UV-B) contained in the extract from the shoots. In addition to preventing and improving skin aging such as spots and wrinkles, it is also possible to prevent and ameliorate skin inflammatory reactions induced by ultraviolet rays and subsequent impairment of skin function. However, the damage recovery agent by ultraviolet (UV-B) irradiation of the present invention should be used for all purposes that are meaningful in exhibiting the damage recovery action by ultraviolet (UV-B) irradiation in addition to these applications. For example, it can be used as a preventive or therapeutic agent for diseases caused by ultraviolet (UV-B) irradiation (such as skin cancer).

  The type I collagen production promoter of the present invention can promote the production of type I collagen through the type I collagen production promoting action of the extract from oil tea. Thereby, the production amount of type I collagen in the skin tissue is increased, and skin aging symptoms such as wrinkles, dullness, disappearance of texture, and decrease in elasticity can be prevented and improved. However, the type I collagen production-promoting agent of the present invention can be used for all purposes that are meaningful for exerting the type I collagen production-promoting action in addition to these uses.

  The profilaggrin production promoter of the present invention promotes the production of profilaggrin in cells through the profilaggrin production promoting action of the extract from oil tea, and increases the amount of filaggrin obtained by hydrolysis of profilagrin, The skin moisturizing ability can be improved, whereby the elasticity of the skin can be maintained, and skin aging, dry skin, wrinkles, rough skin, etc. can be prevented and improved. However, the profilaggrin production promoter of the present invention can be used for all purposes that are meaningful for exerting a profilagrin production promoting action in addition to these uses.

  The filaggrin production promoter of the present invention can promote the production of filaggrin in cells through the filaggrin production promoting action of the extract from oil tea, and can improve the skin moisturizing ability. It can maintain sexuality, and can prevent and improve skin aging, dry skin, wrinkles, rough skin, and the like. However, the filaggrin production promoter of the present invention can be used for all purposes that are meaningful for exerting the filaggrin production promoting action in addition to these uses.

[Skin cosmetic]
The extract from cocoon has laminin 5 production promoting action or damage recovery action by ultraviolet (UV-B) irradiation, and the oil tea extract promotes type I collagen production, profilagulin production promoting action. Alternatively, it has a filaggrin production promoting action, and is excellent in use feeling and safety when applied to the skin. Therefore, the extract from candy and / or oil tea is suitable for blending in skin cosmetics, and in particular, the extract from candy and / or oil tea has an anti-aging effect. Suitable for blending into anti-aging skin cosmetics.

  In this case, the skin cosmetics may be blended with an extract from candy and / or oil tea, or an anti-aging agent formulated from an extract from candy and / or oil tea, extraction from candy. Laminin 5 production promoter formulated from products or damage recovery agent by ultraviolet (UV-B) irradiation, or type I collagen production promoter, profilaggrin production promoter or filaggrin production promotion formulated from oil tea extract An agent may be blended.

  Extracts from spear and / or oil tea, or anti-aging agents formulated from them, laminin 5 production promoter, damage recovery agent by ultraviolet (UV-B) irradiation, type I collagen production promoter, profilagrin By adding a production promoter or filaggrin production promoter to skin cosmetics, laminin 5 production promoting action on skin cosmetics, damage recovery action by ultraviolet (UV-B) irradiation, type I collagen production promoting action, profilagulin production A promoting action or a filaggrin production promoting action can be imparted.

  There are no particular limitations on the types of skin cosmetics that can be blended with the extract from candy and / or oil tea. For example, ointments, creams, emulsions, lotions, packs, jellies, foundations, lip balms, lipsticks, Examples include bath salts.

  In the case where an extract from candy and / or oil tea is blended into the skin cosmetic, the blending amount can be appropriately adjusted according to the type of the skin cosmetic, but a suitable blending ratio is a standard extraction. It is about 0.0001 to 10% by mass in terms of product, and a particularly suitable blending ratio is about 0.001 to 1% by mass in terms of standard extract.

  The skin cosmetic of the present invention has a laminin 5 production promoting action or an ultraviolet ray (UV-B) irradiation recovery action possessed by an extract from a spear or a type I collagen production promoting action possessed by an extract from oil tea, Unless it interferes with filaggrin production promoting action or filaggrin production promoting action, main ingredients, auxiliaries or other ingredients used in the production of normal skin cosmetics, such as astringents, bactericides / antibacterial agents, whitening agents, UV absorbers, Use in combination with moisturizers, cell activators, anti-inflammatory / antiallergic agents, antioxidant / active oxygen scavengers, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, etc. Can do. By using in this way, it becomes a more general product, and a synergistic action with the above-mentioned components used in combination may bring about an excellent effect that is usually expected.

  In addition, the anti-aging agent, laminin 5 production promoter, damage recovery agent by ultraviolet (UV-B) irradiation, type I collagen production promoter, profilaggrin production promoter, filaggrin production promoter or skin cosmetic of the present invention are: Although it is preferably applied to humans, it can also be applied to animals other than humans as long as the respective effects are exhibited.

  Hereinafter, although a manufacture example, a test example, and a compounding example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all.

[Production Example 1] Manufacture of cocoon extract 100 g of seed of bonito was put into 1000 mL of extraction solvent, heated and extracted at 80 ° C for 2 hours with gentle stirring, and then filtered while hot. The obtained filtrate was concentrated under reduced pressure at 40 ° C., and further dried with a vacuum drier to obtain strip extract (samples 1 to 3). Each extraction rate when water, 50 vol% ethanol (volume ratio of water to ethanol = 1: 1) and 80 vol% ethanol (volume ratio of water to ethanol = 1: 4) is used as the extraction solvent. It is shown in 1.

[Production Example 2] Production of oil tea extract 100 g of oil tea leaves were put into 1000 mL of extraction solvent, heated and extracted at 80 ° C for 2 hours with gentle stirring, and then filtered while hot. The obtained filtrate was concentrated under reduced pressure at 40 ° C., and further dried with a vacuum dryer to obtain an oil tea leaf part extract (samples 4 to 6). Each extraction rate when water, 50 vol% ethanol (volume ratio of water to ethanol = 1: 1) and 80 vol% ethanol (volume ratio of water to ethanol = 1: 4) is used as the extraction solvent. It is shown in 2.

[Test Example 1] Laminin 5 production promotion action test The laminin 5 production promotion action was tested as follows for the silkworm extract (sample 2) obtained in Production Example 1.

Normal human epidermal keratinocytes (NHEK) were cultured in an 80 cm ² flask using normal human epidermal keratinocyte medium (KGM) at 37 ° C. and 5% CO ₂ -95% air, and cells were treated with trypsin. Was recovered. The collected cells are diluted with a medium (KGM-BPE) obtained by removing BPE from KGM so that the cell density becomes 1.0 × 10 ⁵ cells / mL, and then seeded at 500 μL per well in a 24-well plate. ° _C., and cultured overnight under conditions of 5% CO 2 -95% air.

After completion of the culture, the medium was removed, and a sample solution (sample 2, sample concentration: 12.5 μg / mL) dissolved in KGM-BPE was added to each well in an amount of 500 μL, and 37 ° C., 5% CO ₂ -95% air. It was cultured for 48 hours under the conditions. After completion of the culture, 100 μL of the supernatant was transferred to an ELISA plate, adsorbed onto the plate at 37 ° C. for 2 hours, and then the solution was discarded, and a phosphate physiological buffer solution (PBS-T) containing 0.05% Tween 20 was used. Washing was performed.

  Thereafter, a blocking operation was performed with a phosphate physiological buffer containing 1% bovine serum albumin. The solution was discarded, washed with phosphate physiological buffer (PBS-T) containing 0.05% Tween 20, and reacted with anti-human laminin 5 antibody (mouse IgG, manufactured by Chemicon). Discard the solution, wash with phosphate buffered saline (PBS-T) containing 0.05% Tween-20, react with the avidin-biotinylated peroxidase complex, perform the same washing procedure, and develop color. Reaction was performed. From the obtained measurement results, the laminin 5 production promotion rate (%) was calculated by the following formula.

Laminin 5 production promotion rate (%) = A / B × 100
In the formula, A represents “absorbance at a wavelength of 405 nm when a sample is added”, and B represents “absorbance at a wavelength of 405 nm when no sample is added”.

  As a result of calculating by the above formula, the laminin 5 production promotion rate of the cocoon extract was 113.4%. From this, it was confirmed that the Koji strip extract has an excellent laminin 5 production promoting action.

[Test Example 2] Damage recovery action test by irradiation with ultraviolet (UV-B) The damage recovery action by ultraviolet (UV-B) irradiation as described below for the stalk extract (sample 2) obtained in Production Example 1 Was tested.

Human normal skin fibroblasts (NB1RGB) were cultured using α-MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells were diluted with α-MEM medium to a cell density of 2.0 × 10 ⁵ cells / mL, and then seeded at 200 μL per well in a 48-well plate. After culturing for 24 hours, the medium was replaced with 100 μL of PBS (−) and irradiated with 1.0 J / cm ² of UV-B. Immediately after the irradiation, PBS (-) was taken out, 400 μL of a sample solution (sample 2, sample concentration: 25 μg / mL) dissolved in 10% FBS-containing D-MEM was added to each well, and cultured for 24 hours.

  The damage recovery effect by ultraviolet (UV-B) irradiation was measured using an MTT assay. After completion of the culture, the medium was removed, and 200 μL of MTT dissolved at a final concentration of 0.4 mg / mL was added to each well. After culturing for 2 hours, blue formazan produced in the cells was extracted with 200 μL of 2-propanol, and after extraction, absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was used as the amount of blue formazan produced. Similarly, after seeding the cells, the cells not irradiated with UV-B and the cells irradiated with UV-B but not added with the sample solution were also measured in the same manner, and were set as a non-irradiated group and an irradiated group, respectively. From the obtained results, the damage recovery rate (%) by ultraviolet (UV-B) irradiation was calculated by the following formula.

Damage recovery rate (%) = {(Nt−C) − (Nt−Sa)} / (Nt−C) × 100
In the above formula, Nt represents “absorbance in cells not irradiated with UV-B”, C represents “absorbance in cells irradiated with UV-B but not added with a sample solution”, and Sa represents “ Absorbance at “cells irradiated with UV-B and added with sample solution” is shown.

  As a result of calculation according to the above formula, the damage recovery rate of the spear extract was 20.6%. From this, it was confirmed that the Koji strip extract has a damage recovery effect by excellent ultraviolet (UV-B) irradiation.

[Test Example 3] Type I collagen production promoting action test The oil tea extract (sample 5) obtained in Production Example 2 was tested for type I collagen production promoting action as follows.

Human normal fibroblasts (NB1RGB) were cultured using Dulbecco's MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells were diluted with the above medium to a cell density of 1.6 × 10 ⁵ cells / mL, then seeded at 100 μL per well in a 96-well microplate, and cultured overnight.

  After completion of the culture, the medium was removed, and 200 μL of each sample (sample 5, sample concentration 100 μg / mL, 25 μg / mL) dissolved in 0.25% FBS-containing Dulbecco MEM medium was added to each well and cultured for 3 days. After culturing, 90 μL of the supernatant was transferred to an ELISA plate and adsorbed on the plate overnight at 4 ° C., then the solution was discarded, and phosphate physiological buffer (PBS-T) containing 0.05% Tween-20 was added. And washed.

  Thereafter, a blocking operation was performed with a phosphate physiological buffer containing 1% bovine serum albumin. The solution was discarded, washed with a phosphate physiological buffer solution (PBS-T) containing 0.05% Tween-20, and reacted with an anti-human collagen type I antibody (rabbit IgG; manufactured by Chemicon). Discard the solution, wash with phosphate physiological buffer solution (PBS-T) containing 0.05% Tween-20, react with HRP-labeled anti-rabbit IgG antibody, and then perform the same washing procedure to develop color. Reaction was performed.

  The rate of promotion of collagen type I production was calculated by the following formula using the above standard ELISA, preparing a calibration curve, and assuming that the amount of collagen type I produced when no sample was added was 100%.

Type I collagen production promotion rate (%) = A / B × 100
In the formula, A represents “amount of type I collagen when a sample is added”, and B represents “amount of type I collagen when no sample is added”.

  As a result of calculation by the above formula, the rate of promotion of type I collagen production of the oil tea extract was 131.3% at a sample concentration of 100 μg / mL and 133.9% at a sample concentration of 25 μg / mL. From this, it was confirmed that the oil tea extract has an excellent type I collagen production promoting action.

[Test Example 4] Profilaggrin / filaggrin production promoting action test The oil tea extract (sample 5) obtained in Production Example 2 was tested for the profilagline / filaggulin production promoting action as follows.

Normal human newborn skin epidermal keratinocytes (NHEK) were cultured in normal human epidermal keratinocyte medium (KGM) in an 80 cm ² flask under conditions of 37 ° C. and 5% CO ₂ -95% air. Cells were collected. The resulting cells were adjusted to a cell density of 1.5 × ¹⁰ 5 cells / mL in the same medium, 37 ° C. and seeded in 2mL each 6-well collagen-coated _plate, 5% CO 2 -95% air For 3 days. After culturing, the medium was replaced with ₂ mL of KGM containing a sample dissolved in 0.5% DMSO (Sample 5, sample concentration: 50 μg / mL), and cultured for 5 days under conditions of 37 ° C., 5% CO ₂ -95% air. did. After completion of the culture, total protein was prepared by a conventional method.

<Western blotting>
The sample adjusted to 10 μg / row was developed by SDS-PAGE and transferred to a PVDF membrane. After blocking with PBS (-) containing 5% skim milk, anti-human filaggrin monoclonal antibody (Harbor Bio-Products), biotin-labeled anti-mouse Ig (Whole Ab, Amersham Biosciences) and streptavidin-peroxidase conjugate The body (CALBIOCHEM) was diluted 1000 times with PBS (-) containing 0.1% Tween20 and 0.3% skim milk, and reacted sequentially, and ECL Western blotting detection reagents and analysis system (Amersham Biosciences) Profilaggrin and filaggrin were detected by luminescence. The detected band was quantitatively measured with KODAK 1D Image Analysis Software EDAS290 Version3.5.

  As a result, the profilaggrin production promoting effect of the sample was evaluated using the value obtained by adding the net intensity (band intensity) of profilagrin and filaggrin in 10 μg of the protein prepared from each of the cells cultured with and without the sample added. The profilaggrin production promotion rate (%) was calculated based on the following formula.

Profilaggrin production promotion rate (%) = A / B × 100
In the above formula, A represents “Net intensity at the time of sample addition (total value of profilagrin and filaggrin)” and B represents “Net intensity at the time of no sample addition (control)”.

  As a result of calculating by the above formula, the profilaggrin production promotion rate of the oil tea extract was 132.6%. From this, it was confirmed that the oil tea extract is excellent in the production promoting action of profilagrin which is a precursor of filaggrin. In addition, since filaggrin is produced by hydrolysis of profilagrin in vivo, an oil tea extract having a profilagrin production promoting action promotes the production of profilagrin and reduces the amount of profilagrin in the cell. By increasing the amount, the amount of filaggrin can also be increased, and as a result, it is considered that filaggrin production promoting action can be exhibited.

[Formulation Example 1]
An emulsion having the following composition was produced by a conventional method.
Agate seed 50% ethanol extract (Production Example 1) 1.0 g
Jojoba oil 4.0g
Olive oil 2.0g
Squalane 2.0g
Cetanol 2.0g
Glyceryl monostearate 2.0g
Polyoxyethylene cetyl ether (20E.O.) 2.5g
Oleic acid polyoxyethylene sorbitan (20E.O.) 2.0g
1,3-butylene glycol 3.0 g
Methyl paraoxybenzoate 0.15g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Formulation Example 2]
A lotion having the following composition was produced by a conventional method.
Strawberry seed part 50% ethanol extract (Production Example 1) 2.2 g
Glycerin 3.0g
1,3-butylene glycol 3.0 g
Oleic acid polyoxyethylene sorbitan (20E.O.) 0.5g
Methyl paraoxybenzoate 0.15g
Citric acid 0.1g
Sodium citrate 0.1g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Composition Example 3]
A cream having the following composition was produced by a conventional method.
Oil tea leaf 50% ethanol extract (Production Example 1) 1.1 g
Liquid paraffin 5.0g
Salami beeswax 4.0g
Cetanol 3.0g
Squalane 10.0g
Lanolin 2.0g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan (20E.O.) 1.5g
3.0 g glyceryl monostearate
1,3-butylene glycol 6.0 g
Fragrance 0.1g
Purified water remainder (total amount is 100 g)

[Formulation Example 4]
A pack having the following composition was produced by a conventional method.
Oil tea leaf 50% ethanol extract (Production Example 1) 5.0 g
Polyvinyl alcohol 15.0g
Polyethylene glycol 3.0g
Propylene glycol 7.0g
Ethanol 10.0g
0.05 g ethyl paraoxybenzoate
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

  Anti-aging agent of the present invention, laminin 5 production promoter, damage recovery agent by ultraviolet (UV-B) irradiation, type I collagen production promoter, profilaggulin production promoter, filaggrin production promoter and skin cosmetics Can greatly contribute to the prevention or improvement of aging.

Claims (7)

  An anti-aging agent characterized by containing an extract from bonito and / or oil tea as an active ingredient.   A laminin 5 production promoter characterized by containing an extract from cocoon as an active ingredient.   An agent for recovering damage caused by irradiation with ultraviolet rays (UV-B), which comprises an extract from cocoon as an active ingredient.   A type I collagen production promoter comprising an extract from oil tea as an active ingredient.   A profilagulin production promoter characterized by containing an extract from oil tea as an active ingredient.   A filaggrin production promoter characterized by containing an extract from oil tea as an active ingredient.   A skin cosmetic characterized by blending an extract from candy and / or oil tea.