CN111067860A - Stem cell secretion factor mask for whitening skin and fading acne marks and preparation method thereof - Google Patents
Stem cell secretion factor mask for whitening skin and fading acne marks and preparation method thereof Download PDFInfo
- Publication number
- CN111067860A CN111067860A CN202010053757.2A CN202010053757A CN111067860A CN 111067860 A CN111067860 A CN 111067860A CN 202010053757 A CN202010053757 A CN 202010053757A CN 111067860 A CN111067860 A CN 111067860A
- Authority
- CN
- China
- Prior art keywords
- stem cell
- mask
- cell secretion
- vitamin
- whitening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000028327 secretion Effects 0.000 title claims abstract description 49
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 43
- 208000002874 Acne Vulgaris Diseases 0.000 title claims abstract description 39
- 206010000496 acne Diseases 0.000 title claims abstract description 39
- 230000002087 whitening effect Effects 0.000 title claims abstract description 35
- 238000005562 fading Methods 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title abstract description 10
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 44
- 210000004027 cell Anatomy 0.000 claims abstract description 37
- 239000000843 powder Substances 0.000 claims abstract description 36
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 34
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 34
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 34
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims abstract description 27
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229930003537 Vitamin B3 Natural products 0.000 claims abstract description 25
- 229960003512 nicotinic acid Drugs 0.000 claims abstract description 25
- 235000019160 vitamin B3 Nutrition 0.000 claims abstract description 25
- 239000011708 vitamin B3 Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 22
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 22
- 239000011718 vitamin C Substances 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 235000011399 aloe vera Nutrition 0.000 claims abstract description 18
- 238000004113 cell culture Methods 0.000 claims abstract description 17
- 241001116389 Aloe Species 0.000 claims abstract description 16
- 239000008213 purified water Substances 0.000 claims abstract description 16
- -1 hydrolat Substances 0.000 claims abstract description 3
- 230000001815 facial effect Effects 0.000 claims description 45
- 241000282414 Homo sapiens Species 0.000 claims description 23
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 15
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 210000003954 umbilical cord Anatomy 0.000 claims description 9
- 230000003248 secreting effect Effects 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 6
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 claims description 5
- 235000010254 Jasminum officinale Nutrition 0.000 claims description 5
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 claims description 5
- 241000220317 Rosa Species 0.000 claims description 5
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 5
- 239000001569 carbon dioxide Substances 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000004017 serum-free culture medium Substances 0.000 claims description 5
- 238000000684 flow cytometry Methods 0.000 claims description 2
- 240000005385 Jasminum sambac Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 21
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 abstract description 8
- 239000002537 cosmetic Substances 0.000 abstract description 4
- 239000002904 solvent Substances 0.000 abstract description 4
- 238000004108 freeze drying Methods 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 56
- 239000000306 component Substances 0.000 description 19
- 230000032683 aging Effects 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 9
- 230000037303 wrinkles Effects 0.000 description 8
- 239000000047 product Substances 0.000 description 6
- 241000207840 Jasminum Species 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000002780 melanosome Anatomy 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000003712 anti-aging effect Effects 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 201000010251 cutis laxa Diseases 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 239000002932 luster Substances 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 230000003020 moisturizing effect Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 239000000341 volatile oil Substances 0.000 description 3
- 244000144927 Aloe barbadensis Species 0.000 description 2
- 235000002961 Aloe barbadensis Nutrition 0.000 description 2
- 201000004624 Dermatitis Diseases 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 108010059881 Lactase Proteins 0.000 description 2
- 208000012641 Pigmentation disease Diseases 0.000 description 2
- 229930003270 Vitamin B Natural products 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000000172 allergic effect Effects 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 210000004177 elastic tissue Anatomy 0.000 description 2
- 239000003925 fat Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088592 immunologic factor Drugs 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 210000002752 melanocyte Anatomy 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000004877 mucosa Anatomy 0.000 description 2
- 210000003097 mucus Anatomy 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 235000005152 nicotinamide Nutrition 0.000 description 2
- 239000011570 nicotinamide Substances 0.000 description 2
- 229960003966 nicotinamide Drugs 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000019612 pigmentation Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 230000009759 skin aging Effects 0.000 description 2
- 235000019156 vitamin B Nutrition 0.000 description 2
- 239000011720 vitamin B Substances 0.000 description 2
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000009024 Epidermal Growth Factor Human genes 0.000 description 1
- 101800003838 Epidermal growth factor Proteins 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- 239000001263 FEMA 3042 Substances 0.000 description 1
- 108090000386 Fibroblast Growth Factor 1 Proteins 0.000 description 1
- 102100031706 Fibroblast growth factor 1 Human genes 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000006877 Insect Bites and Stings Diseases 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 208000019255 Menstrual disease Diseases 0.000 description 1
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 1
- 206010051246 Photodermatosis Diseases 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 206010040829 Skin discolouration Diseases 0.000 description 1
- 206010043189 Telangiectasia Diseases 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 229940006091 aloe polysaccharide Drugs 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 230000032677 cell aging Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 102000013035 dynein heavy chain Human genes 0.000 description 1
- 108060002430 dynein heavy chain Proteins 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 229940116977 epidermal growth factor Drugs 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000006355 external stress Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000002267 hypothalamic effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 239000000367 immunologic factor Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000003780 keratinization Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 210000004088 microvessel Anatomy 0.000 description 1
- 230000005709 nerve cell growth Effects 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003076 paracrine Effects 0.000 description 1
- 210000005037 parasympathetic nerve Anatomy 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000008845 photoaging Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000037370 skin discoloration Effects 0.000 description 1
- 230000004215 skin function Effects 0.000 description 1
- 230000008470 skin growth Effects 0.000 description 1
- 230000036559 skin health Effects 0.000 description 1
- 230000036548 skin texture Effects 0.000 description 1
- 230000003860 sleep quality Effects 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000036561 sun exposure Effects 0.000 description 1
- 239000000516 sunscreening agent Substances 0.000 description 1
- 230000002889 sympathetic effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 229920002258 tannic acid Polymers 0.000 description 1
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 1
- 229940033123 tannic acid Drugs 0.000 description 1
- 235000015523 tannic acid Nutrition 0.000 description 1
- 208000009056 telangiectasis Diseases 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
- A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The invention belongs to the technical field of cosmetic preparation, and particularly relates to a stem cell secretion factor mask for whitening and fading acne marks and a preparation method thereof. A stem cell secretion factor mask for whitening and fading acne marks is characterized in that mask liquid of the mask consists of the following components: the dry cell secretion factor powder, hyaluronic acid, hydrolat, vitamin C, vitamin B3, aloe powder and purified water. The stem cell secretion factor contained in the invention is prepared by freeze-drying the cell culture solution at low temperature, thus fully ensuring the activity of various stem cell secretion factors contained in the cell culture solution. Vitamin C, vitamin B3, hyaluronic acid, and hydrolat can be used as solvent to maintain activity of stem cell secretion factor for a long time, and solute and various components in the solvent do not react with each other, and can give full play to skin care effect, inhibit melanin generation of skin tissue, and reduce acne mark.
Description
Technical Field
The invention belongs to the technical field of cosmetic preparation, and particularly relates to a stem cell secretion factor mask for whitening and fading acne marks and a preparation method thereof.
Background
Skin aging refers to the aging damage of skin function, which reduces the protection ability and regulation ability of skin to human body, so that the skin can not adapt to the change of internal and external environment, and the change of overall appearance conditions such as color, luster, shape, texture, etc. appears. Aging of the skin is classified into intrinsic aging and extrinsic aging. Endogenous aging refers to the natural aging of the skin as it ages. It is manifested as whitening of the skin, fine wrinkles, decreased elasticity, skin laxity, etc. The most prominent cause of extrinsic aging is photoaging due to sun exposure. Skin discoloration manifested as wrinkles, loose skin, roughness, yellowish or grayish yellow, telangiectasia, formation of pigmented spots, and the like.
The conventional cosmetics are almost prepared by mixing chemical organic solvents, can only solve the problems of water shortage and pigmentation on the surface of skin, and can treat the symptoms and the root causes. Once discontinued, it is highly recurrent, but prolonged or frequent use can cause significant damage to the skin tissue.
In general skin care products, components which are easily sensitized such as perfumes, preservatives, and coloring matters are added. The addition of chemical sunscreen agents or powders, whitening components, acne-removing and bacteriostatic components, or macromolecular functional components obtained by pulling and tightening in special skin care products can result in more or less sensitive and even allergic skin.
Similarly, if the quality of basic ingredients such as oils and fats used in skin care products is poor, they are not easily absorbed by human skin, and acne, dermatitis, and the like are easily caused.
Some bioactive substances are gradually found to have whitening and anti-aging effects on human skin, such as hydrolat, stem cell secretion factor, vitamin C, vitamin B3, low molecular weight hyaluronic acid, aloe powder and the like.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
In order to make various skin care product raw materials give full play to the functions and overcome the defect caused by using a certain component singly, the facial mask integrates the advantages of the raw materials, and makes the facial mask give full play to the skin care effects of whitening and removing acne marks.
The invention provides the following technical scheme:
a stem cell secretion factor mask for whitening and fading acne marks is characterized in that mask liquid of the mask consists of the following components: the dry cell secretion factor powder, hyaluronic acid, hydrolat, vitamin C, vitamin B3, aloe powder and purified water.
Preferably, the facial mask liquid of the facial mask comprises the following components in percentage by mass: 0.1-2% of dry cell secretion factor powder, 10-15% of hyaluronic acid, 5-20% of hydrolat, 1-4% of vitamin C, 1-3% of vitamin B3, 5-10% of aloe powder and the balance of purified water.
The invention also provides a preparation method of the stem cell secretion factor facial mask for whitening and fading acne marks, which comprises the following steps:
(1) separating and culturing human umbilical cord mesenchymal stem cells, culturing the cells in a culture bottle, and collecting cell culture solution when the coverage rate of the cells reaches 90%;
(2) freezing and drying the cell culture solution collected in the step (1) to obtain stem cell secretory factor powder;
(3) uniformly mixing 0.1-2% of dry cell secretion factor powder, 10-15% of hyaluronic acid, 5-20% of hydrolat, 1-4% of vitamin C, 1-3% of vitamin B3, 5-10% of aloe powder and purified water according to the mass ratio to prepare mask liquid;
(4) and (4) soaking the facial mask paper in the facial mask liquid prepared in the step (3) for 5-6min, so that the facial mask paper can fully absorb the facial mask liquid to prepare the stem cell secretory factor facial mask for whitening and fading acne marks.
Preferably, the culture medium of the human umbilical cord mesenchymal stem cells in the step (1) is MSC-T4 serum-free culture medium,flow cytometry detection of CD 7398.65%, CD 9099.82%, CD 10598.07%, CD14 for P4 generation-0.00％，CD34-0.00％，CD19-0.01％，CD45-0.02％，HLA-DR-0.10％。
Preferably, the mesenchymal stem cell culture conditions in step (1) are 37 ℃ of temperature, 5% of carbon dioxide concentration and 99% of humidity.
Preferably, the hyaluronic acid in step (3) is a low molecular weight hyaluronic acid.
Preferably, the molecular weight range of the low molecular weight hyaluronic acid is as follows: 10000-500000.
Preferably, the hydrolat in the step (3) is jasmine hydrolat or rose hydrolat.
Compared with the prior art, the invention has the following beneficial effects:
the stem cell secretion factor contained in the invention is prepared by freeze-drying the cell culture solution at low temperature, thus fully ensuring the activity of various stem cell secretion factors contained in the cell culture solution. Vitamin C, vitamin B3, hyaluronic acid, and hydrolat can be used as solvent to maintain activity of stem cell secretion factor for a long time, and solute and various components in the solvent do not react with each other, and can give full play to skin care effect, inhibit melanin generation of skin tissue, and reduce acne mark.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
The stem cell culture fluid contains the following stem cell secretion factors which are full of vitality. Various active cytokines play a large role through the paracrine action of cells.
① embryonic cell differentiation factor for activating and reviving human stem cells in dormant state to make them regenerate, reverse aging, reappear youth, prolong life and improve life quality
② nerve cell growth factor for activating and reviving human nerve stem cell in dormant state to make nervous system develop again, so as to enhance thinking and analysis ability, enhance memory, regulate sympathetic nerve and parasympathetic nerve to restore normal work of biological clock, improve sleep, and improve sleep quality.
③ the inducer of immune factor can promote the immune system of human body to generate more immune factors and improve the disease preventing and resisting ability of human body.
④ epidermal growth factor is the main motive element for skin growth and elastic fiber synthesis, and has effects of effectively generating and strengthening tissue fiber, rapidly improving skin metabolism state, promoting skin superficial layer, middle layer and deep layer tissue cell metabolism, promoting the synthesis of elastic fiber and collagen, and removing wrinkle, caring skin, and beautifying skin.
⑤ the inhibitor of the enzyme lactase has effects of inhibiting the synthesis of the enzyme lactase, reducing the generation of melanin, and thus has the effects of removing speckle, and whitening skin.
⑥ vascular endothelial cell growth factor, which can enrich the subcutaneous microvascular network, comb the skin tissue nutrition supply channel, improve the living microenvironment of skin tissue cells, make the nutrient components such as nucleic acid fully supplied to the new cells, make the tissue cells in the best physiological state, prevent aging, and reduce the occurrence of cardiovascular and cerebrovascular diseases.
⑦ fat trend factor, which can induce normal adipose tissue of human body to move to correct position, and has effects of enlarging breast, lifting hip, reducing fat, improving bloated state, and recovering body posture.
⑧ hormone prohormone can stimulate hypothalamic cell to balance nine major hormones in human body, and improve endocrine disturbance caused by external stress and irregular life, and can be used for treating mottle, acne, constipation, menoxenia, and sexual interest decrease.
⑨ DNA is an indispensable.
⑩ the protein glue element can make connective tissue store water, prevent tissue water molecule loss from causing dryness, wrinkle, loss of luster and elasticity, and make skin plump and tender.
Hyaluronic acid is a multifunctional matrix, and is widely distributed in various parts of the human body. Wherein the skin also contains a significant amount of hyaluronic acid. The skin aging process of human beings also changes with the content and metabolism of hyaluronic acid, it can improve skin nutrition metabolism, make skin tender and smooth, remove wrinkle, increase elasticity, prevent aging, and is good transdermal absorption enhancer while keeping moisture. The nutrient can be used together with other nutrient components to achieve the more ideal effect of promoting nutrient absorption.
Vitamin C is one of the most important factors for maintaining skin health, can eliminate dull skin color, recover skin luster and tightness, maintain the natural moisturizing function of the skin and effectively eliminate free radicals. The emulsion containing vitamin C can directly permeate skin to repair damage caused by ultraviolet rays, so that the skin can be whitened, and fine wrinkles and black spots can be reduced. Recently, vitamin C is frequently used in high concentration for skin treatment, and can improve the effects of acne treatment, whitening and the like.
Vitamin B3 is also a well-known skin anti-aging ingredient in the field of beauty skin science, and is more and more important in recent years, and the most important effect of vitamin B3 in the aspect of skin anti-aging is to relieve and prevent dark complexion, yellowing and vegetable color of skin generated in the early aging process. Of course, vitamin B3 contributes much more than this to the skin and also repairs the damaged stratum corneum lipid barrier, increasing skin resistance. The skin care product containing the niacinamide has strong water locking and moisturizing effects. When the vitamin B3 action concentration is 0.25 and 1.25mg/ml, the movement rate of melanosomes in melanocytes is obviously increased (P <0.05), and the concentration of melanosomes in cells is reduced along with the increase of the vitamin B3 concentration. And (4) conclusion: vitamin B3 participates in the transfer process of melanin and can accelerate the movement rate of melanosomes in melanocytes, which shows that vitamin B3 has the regulation and control functions on calcium pumps, the expression of cell dynamic protein Dynein and the movement of melanosomes, and the action concentration is an important regulation factor. With age, the skin becomes dark yellow and dull, which can be improved by topical niacinamide. Randomized studies showed significant improvement in skin texture and significant reduction in fine lines and wrinkles at week 8 with 2 vitamin B3 stocks per day; the skin became elastic at week 12, with a marked reduction in pigmentation and erythema, a marked reduction in dark yellow and a shiny appearance.
The pure dew water contains various precious components of plants, and about 0.3-0.5% of water-soluble components of essential oil, so that the pure dew water still has the fragrance, partial curative effect and slight antibacterial property of the essential oil, and contains plant essence (such as tannic acid and flavonoid) which is not contained in the essential oil, so that the pure dew water has the effect of conditioning skin, and the low-concentration characteristic of the pure dew water enables the pure dew water to be absorbed by the skin more easily.
The aloe polysaccharide and vitamins have good nourishing, moistening and whitening effects on human skin. Aloe barbadensis Miller is the fresh aloe leaf most suitable for direct skin care, i.e., Aloe barbadensis Miller, and has skin astringent, softening, moisturizing, antiinflammatory, and bleaching effects. It also has effects in relieving sclerosis and keratinization, improving scar, preventing wrinkle, eye pouch, and cutis laxa, keeping skin moist and delicate, treating skin inflammation, and treating acne, freckle, acne, scald, knife wound, and insect bite. The mucus (protein) in aloe is a polysaccharide such as arboran A Baloannanaloetin as a core component, and the mucus is an important component for preventing cell aging and treating chronic allergy. Mucin exists in human muscle and gastrointestinal mucosa, and makes the tissue elastic, and if insufficient mucin is available, the muscle and mucosa lose elasticity and become rigid and aging.
Example 1
A stem cell secretion factor mask for whitening and fading acne marks comprises the following components in percentage by mass: 0.1% of dry cell secretion factor powder, 10% of hyaluronic acid, 5% of hydrolat, 1% of vitamin C, 1% of vitamin B3 and 5% of aloe powder, and the balance of purified water.
The preparation method of the stem cell secretion factor mask for whitening and fading acne marks comprises the following steps:
(1) separating and culturing human umbilical cord mesenchymal stem cells, culturing the cells in a culture bottle, and collecting cell culture solution when the coverage rate of the cells reaches 90%; the culture medium of the human umbilical cord mesenchymal stem cells is MSC-T4 serum-free culture medium, and the detection of CD 7398.65%, CD 9099.82%, CD 10598.07% and CD14 by P4 generation cell flow cytometry-0.00％，CD34-0.00％，CD19-0.01％，CD45-0.02％，HLA-DR-0.10 percent; the culture conditions of the mesenchymal stem cells are that the temperature is 37 ℃, the concentration of carbon dioxide is 5 percent, and the humidity is 99 percent;
(2) freezing and drying the cell culture solution collected in the step (1) to obtain stem cell secretory factor powder;
(3) uniformly mixing 0.1% of dry cell secretion factor powder, 10% of hyaluronic acid, 5% of hydrolat, 1% of vitamin C, 1% of vitamin B3, 5% of aloe powder and purified water according to the mass ratio to prepare facial mask liquid; the hyaluronic acid is low molecular hyaluronic acid with the molecular weight range of 10000-500000; the hydrolat is jasmine hydrolat or rose hydrolat;
(4) and (4) soaking the facial mask paper in the facial mask liquid prepared in the step (3) for 5min, so that the facial mask paper can fully absorb the facial mask liquid to prepare the stem cell secretion factor facial mask for whitening and fading acne marks.
Example 2
A stem cell secretion factor mask for whitening and fading acne marks comprises the following components in percentage by mass: 2% of dry cell secretion factor powder, 15% of hyaluronic acid, 20% of hydrolat, 4% of vitamin C, 3% of vitamin B3 and 10% of aloe powder, and the balance of purified water.
The preparation method of the stem cell secretion factor mask for whitening and fading acne marks comprises the following steps:
(1) separating and culturing human umbilical cord mesenchymal stem cells, culturing the cells in a culture bottle, and collecting cell culture solution when the coverage rate of the cells reaches 90%; the human umbilical cordThe culture medium of the mesenchymal stem cells is MSC-T4 serum-free culture medium, and the detection of CD 7398.65%, CD 9099.82%, CD 10598.07% and CD14 by P4 generation cell flow cytometry-0.00％，CD34-0.00％，CD19-0.01％，CD45-0.02％，HLA-DR-0.10 percent; the culture conditions of the mesenchymal stem cells are that the temperature is 37 ℃, the concentration of carbon dioxide is 5 percent, and the humidity is 99 percent;
(2) freezing and drying the cell culture solution collected in the step (1) to obtain stem cell secretory factor powder;
(3) uniformly mixing 2% of dry cell secretion factor powder, 15% of hyaluronic acid, 20% of hydrolat, 4% of vitamin C, 3% of vitamin B3, 10% of aloe powder and purified water according to the mass ratio to prepare facial mask liquid; the hyaluronic acid is low molecular hyaluronic acid with the molecular weight range of 10000-500000; the hydrolat is jasmine hydrolat or rose hydrolat;
(4) and (4) soaking the facial mask paper in the facial mask liquid prepared in the step (3) for 5min, so that the facial mask paper can fully absorb the facial mask liquid to prepare the stem cell secretion factor facial mask for whitening and fading acne marks.
Example 3
A stem cell secretion factor mask for whitening and fading acne marks comprises the following components in percentage by mass: 1% of dry cell secretion factor powder, 12% of hyaluronic acid, 15% of hydrolat, 2% of vitamin C, 2% of vitamin B3 and 8% of aloe powder, and the balance of purified water.
The preparation method of the stem cell secretion factor mask for whitening and fading acne marks comprises the following steps:
(1) separating and culturing human umbilical cord mesenchymal stem cells, culturing the cells in a culture bottle, and collecting cell culture solution when the coverage rate of the cells reaches 90%; the culture medium of the human umbilical cord mesenchymal stem cells is MSC-T4 serum-free culture medium, and the detection of CD 7398.65%, CD 9099.82%, CD 10598.07% and CD14 by P4 generation cell flow cytometry-0.00％，CD34-0.00％，CD19-0.01％，CD45-0.02％，HLA-DR-0.10 percent; the mesenchymal stem cell culture stripThe temperature is 37 ℃, the concentration of carbon dioxide is 5%, and the humidity is 99%;
(2) freezing and drying the cell culture solution collected in the step (1) to obtain stem cell secretory factor powder;
(3) uniformly mixing 1% of dry cell secretion factor powder, 12% of hyaluronic acid, 15% of hydrolat, 2% of vitamin C, 2% of vitamin B3, 8% of aloe powder and purified water according to the mass ratio to prepare facial mask liquid; the hyaluronic acid is low molecular hyaluronic acid with the molecular weight range of 10000-500000; the hydrolat is jasmine hydrolat or rose hydrolat;
(4) and (4) soaking the facial mask paper in the facial mask liquid prepared in the step (3) for 6min, so that the facial mask paper can fully absorb the facial mask liquid to prepare the stem cell secretion factor facial mask for whitening and fading acne marks.
Investigation of use effect:
the using method comprises the following steps: after cleaning and wiping the face before sleeping at night every day, respectively taking a piece of facial mask paper (purchased from Shanghai platong cosmetics special agency) and soaking the facial mask paper with 8ml of facial mask liquid prepared in the embodiment 1-3 for 5min, uniformly coating the facial mask liquid left after soaking the facial mask paper on the whole face, and applying the facial mask on the face for 15-20min to ensure that the facial skin completely absorbs the facial mask liquid. After 1 month, the face was observed, 100 cases each, and the results of the examination are shown in Table 1 below.
3 control examples, control 1, control 2 and control 3, were set simultaneously, wherein:
the mask solution of the mask in the comparative example 1 comprises the following components in percentage by mass: 0.1% of dry cell secretion factor powder, 10% of hyaluronic acid, 5% of hydrolat, 1% of vitamin C and 1% of vitamin B, and the balance of purified water.
The mask solution of the mask in the comparative example 2 comprises the following components in percentage by mass: 2% of dry cell secretion factor powder, 15% of hyaluronic acid, 20% of hydrolat, 4% of vitamin C, 3% of vitamin B3 and the balance of purified water.
The mask solution of the mask in the comparative example 3 comprises the following components in percentage by mass: 1% of dry cell secretion factor powder, 12% of hyaluronic acid, 15% of hydrolat, 2% of vitamin C and 2% of vitamin B, and the balance of purified water.
Table 1 investigation of the use effect of the skin care product of the present invention
As can be seen from Table 1, in each of 100 trial cases, none of 1 of examples 1-3 had facial discomfort and other allergic phenomena; at least 3 cases of the skin is fine and smooth and has no obvious change; at least 90 cases of skin become white obviously and the acne marks and the color spots disappear; at least 50 cases of pore reduction; at least 97 cases of facial skin are obviously fine and glossy.
Compared with the control example, the skin is obviously whitened, and the number of cases of acne marks and color spots disappearing is obviously higher than that of the control example, which shows that the stem cell secretion factor facial mask for whitening and fading the acne marks has better whitening and acne mark fading effects.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (8)
1. The stem cell secretion factor mask for whitening and fading acne marks is characterized by comprising the following components: the dry cell secretion factor powder, hyaluronic acid, hydrolat, vitamin C, vitamin B3, aloe powder and purified water.
2. The stem cell secretion factor mask for whitening and fading the acne marks according to claim 1, wherein the mask solution of the mask comprises the following components in percentage by mass: 0.1-2% of dry cell secretion factor powder, 10-15% of hyaluronic acid, 5-20% of hydrolat, 1-4% of vitamin C, 1-3% of vitamin B3, 5-10% of aloe powder and the balance of purified water.
3. A method for preparing the stem cell secretion factor mask for whitening and lightening acne marks according to claim 1 or 2, which comprises the following steps:
separating and culturing human umbilical cord mesenchymal stem cells, culturing the cells in a culture bottle, and collecting cell culture solution when the coverage rate of the cells reaches 90%;
freezing and drying the cell culture solution collected in the step (1) to obtain stem cell secretory factor powder;
uniformly mixing 0.1-2% of dry cell secretion factor powder, 10-15% of hyaluronic acid, 5-20% of hydrolat, 1-4% of vitamin C, 1-3% of vitamin B3 and 5-10% of aloe powder with purified water according to the mass ratio to prepare mask liquid;
and (4) soaking the facial mask paper in the facial mask liquid prepared in the step (3) for 5-6min, so that the facial mask paper can fully absorb the facial mask liquid to prepare the stem cell secretory factor facial mask for whitening and fading acne marks.
4. The method for preparing the facial mask containing the stem cell secretion factors for whitening and lightening the acne marks as claimed in claim 3, wherein the culture medium of the human umbilical cord mesenchymal stem cells in the step (1) is MSC-T4 serum-free culture medium, and the detection of CD 7398.65%, CD 9099.82%, CD 10598.07% and CD14 by the P4 generation cell flow cytometry is carried out-0.00%，CD34-0.00%，CD19-0.01%，CD45-0.02%，HLA-DR-0.10%。
5. The method for preparing the stem cell secretion factor mask for whitening and lightening acne marks according to claim 3, wherein the mesenchymal stem cell culture conditions in the step (1) are 37 ℃ of temperature, 5% of carbon dioxide concentration and 99% of humidity.
6. The method for preparing a stem cell secretion factor mask for whitening and lightening acne marks according to claim 3, wherein the hyaluronic acid in the step (3) is low molecular hyaluronic acid.
7. The method for preparing the stem cell secretion factor mask for whitening and lightening acne marks according to claim 6, wherein the molecular weight range of the low molecular hyaluronic acid is as follows: 10000-500000.
8. The method for preparing a mask containing stem cell secretion factors for whitening and lightening acne marks according to claim 3, wherein the hydrolat in the step (3) is jasmine hydrolat or rose hydrolat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010053757.2A CN111067860A (en) | 2020-01-17 | 2020-01-17 | Stem cell secretion factor mask for whitening skin and fading acne marks and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010053757.2A CN111067860A (en) | 2020-01-17 | 2020-01-17 | Stem cell secretion factor mask for whitening skin and fading acne marks and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111067860A true CN111067860A (en) | 2020-04-28 |
Family
ID=70323864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010053757.2A Pending CN111067860A (en) | 2020-01-17 | 2020-01-17 | Stem cell secretion factor mask for whitening skin and fading acne marks and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111067860A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116392575A (en) * | 2023-03-22 | 2023-07-07 | 广东唯泰生物科技有限公司 | Preparation for treating acne by combining stem cell active ingredients with hyaluronic acid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110251453A (en) * | 2019-07-22 | 2019-09-20 | 广西哇善妙生物科技有限公司 | It is a kind of for smoothing wrinkle, whitening and the stem cell secretion factor skin care item and preparation method thereof for desalinating acne print |
-
2020
- 2020-01-17 CN CN202010053757.2A patent/CN111067860A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110251453A (en) * | 2019-07-22 | 2019-09-20 | 广西哇善妙生物科技有限公司 | It is a kind of for smoothing wrinkle, whitening and the stem cell secretion factor skin care item and preparation method thereof for desalinating acne print |
Non-Patent Citations (2)
| Title |
|---|
| 卫生部: "《化妆品安全技术规范》", 1 December 2016 * |
| 国家药品监督管理局: "《化妆品监督管理常见问题解答（一）》", 10 January 2019 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116392575A (en) * | 2023-03-22 | 2023-07-07 | 广东唯泰生物科技有限公司 | Preparation for treating acne by combining stem cell active ingredients with hyaluronic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111658818B (en) | Medical repair dressing and application thereof | |
| CN104173251B (en) | A kind of bone glue of yak former peptide cleawhite pack and preparation method thereof | |
| CN111840144A (en) | Moisturizing and repairing composition, skin care product and preparation method thereof | |
| CN111643411B (en) | Anti-aging composition and application thereof | |
| CN108125892A (en) | A kind of marine organism moisture-retention anti-aging cosmetics composition | |
| CN109330954B (en) | Whitening skin brightening lotion, preparation method thereof and tyrosinase inhibitor | |
| CN115040461A (en) | Moisturizing and hydrating fresh freeze-dried mask | |
| CN104490713A (en) | Super-soft living spring skin care product with functions of removing foul, tightening, resisting wrinkles and activating skin | |
| CN113384482B (en) | Moisturizing and skin-tendering essence and preparation method thereof | |
| CN110559226A (en) | Whitening lotion and preparation method thereof | |
| CN105560092A (en) | Micro-repairing cream and preparation method thereof | |
| CN104434642A (en) | Whitening and moisturizing gel for after-sun repairing and preparation method thereof | |
| CN113081942A (en) | Multi-dimensional hydration cream containing apple stem cells | |
| CN111067860A (en) | Stem cell secretion factor mask for whitening skin and fading acne marks and preparation method thereof | |
| CN111759762A (en) | Black eye removing composition, essence and preparation method thereof | |
| CN116617138A (en) | Anti-wrinkle whitening composition containing marine protein, and preparation method and application thereof | |
| CN111012699A (en) | Antioxidant oil-controlling emulsion containing fucoidan-kelp extract | |
| CN110859758A (en) | Whitening anti-aging repair type medical cold compress patch and preparation method thereof | |
| CN114712285B (en) | Composition with anti-wrinkle effect and preparation method and application thereof | |
| CN108836904A (en) | A kind of skin care compositions of wrinkle reduction and the preparation method and application thereof | |
| CN113368003A (en) | Composition with overnight muscle repairing effect and preparation method and application thereof | |
| CN111544335A (en) | Essence containing soluble collagen and malachite extract and preparation method thereof | |
| CN112618404A (en) | Application of sandalwood polypeptide in preparation of skin care product and composition thereof | |
| CN112402325A (en) | Safflower polypeptide reverse-aging cream | |
| CN112057395A (en) | Makeup removing mask containing plukenetia volubilis linneo |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200428 |