CN110713417A - Plant source disease-resistant growth promoter for resisting wheat root rot and application thereof - Google Patents

Plant source disease-resistant growth promoter for resisting wheat root rot and application thereof Download PDF

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Publication number
CN110713417A
CN110713417A CN201911188127.XA CN201911188127A CN110713417A CN 110713417 A CN110713417 A CN 110713417A CN 201911188127 A CN201911188127 A CN 201911188127A CN 110713417 A CN110713417 A CN 110713417A
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wheat
disease
root rot
plant
growth promoter
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张玉凤
刘苹
刘兆辉
田慎重
李庆凯
李燕
边文范
董亮
郭洪海
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Institute of Agricultural Resources and Environment of Shandong Academy of Agricultural Sciences
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C5/00Fertilisers containing other nitrates
    • C05C5/02Fertilisers containing other nitrates containing sodium or potassium nitrate
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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Abstract

The invention discloses a plant source disease-resistant growth promoter for resisting wheat root rot and application thereof. The invention adopts pyroligneous acid which is a product of plant pyrolysis gasification as a disease-resistant raw material, and simultaneously adds organic humic acid which promotes the growth of plant root systems, thereby developing a plant source disease-resistant growth promoter which resists wheat root rot and a high-efficiency application method thereof.

Description

Plant source disease-resistant growth promoter for resisting wheat root rot and application thereof
Technical Field
The invention relates to a plant source disease-resistant growth promoter for resisting wheat root rot and application thereof, belonging to the field of plant growth regulators.
Background
Wheat root rot is a soil-borne disease caused by various pathogenic fungi, and fusarium and helminthosporium are common. These pathogenic bacteria can survive in soil for a long time and can occur during the whole growth period of wheat, and once the conditions are proper, the disease condition is easy to expand to cause spreading disasters. Generally, the yield of the wheat can be reduced by 20-30 percent, and can reach more than 50 percent in severe cases, so that the yield of the wheat is seriously influenced. The wheat root rot is not easy to be found in the early infection stage, and the optimal control period is missed when obvious symptoms appear on the overground part. In agricultural production, the main method for preventing and treating the diseases is chemical prevention and treatment. However, with the degradation of chemical pesticides, the generation of drug resistance by pathogenic bacteria, and the aggravation of the harm of chemical pesticides to the environment and human beings, the development and utilization of safe and effective control methods are urgent.
Biological pesticides are increasingly important in the prevention and treatment of plant diseases because of their advantages of being non-toxic, pollution-free and residue-free. For example, patent CN102057914A discloses a bactericidal composition containing metconazole and antibiotic compounds, and the antibiotics include validamycin, polyoxin, etc., and can be used for preventing and treating powdery mildew, downy mildew, gray mold, etc. Patent CN105284821A discloses a pesticide composition for preventing and treating wheat scab, which contains 1-150 parts by weight of validamycin and 1-20 parts by weight of metconazole per mu, and has obvious synergistic effect on wheat scab. The botanical pesticide is one of the first choice of green biological pesticide by virtue of the advantages of easy degradation and no pollution in natural environment. But the reports on the control of wheat root rot are less. At present, researches show that the pyroligneous liquor as a product of plant pyrolysis gasification has certain bacteriostatic activity on plant pathogenic bacteria, but the researches are only limited to the bacteriostatic research on the pathogenic bacteria per se, and reports on how the pyroligneous liquor is prepared into products which are marketed and applied in the field are few. For example, in the production process and production line of a wood vinegar composite preparation of patent application publication No. CN 110330994A, refined wood vinegar is emulsified and then mixed with auxiliary materials to prepare the wood vinegar composite preparation. The patent mainly describes a preparation method of the wood vinegar, and does not relate to the field application of the wood vinegar compound preparation.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides a plant-derived disease-resistant growth promoter for resisting wheat root rot and application thereof. The invention adopts pyroligneous acid which is a product of plant pyrolysis gasification as a disease-resistant raw material, and simultaneously adds organic humic acid which promotes the growth of plant root systems, thereby developing a plant source disease-resistant growth promoter which resists wheat root rot and a high-efficiency application method thereof.
A plant source disease-resistant growth promoter for resisting wheat root rot comprises the following raw materials in parts by weight:
55-80 parts of pyroligneous, 6-9 parts of potassium fulvate, 8-19 parts of urea and 3-19 parts of potassium nitrate.
Further, the preparation method of the wood vinegar comprises the following steps: the wood chips and the cotton straws are put into an improved biomass gasification generating furnace described in the patent with the application number of CN 109694749A according to the mass ratio of 1-2:1, vaporization treatment is carried out at the temperature of 740-.
Further, the potassium fulvate is produced according to the method disclosed in the patent with the patent number of CN 108070008 and the patent name of the method for preparing the fulvate by activating lignite with the composite photocatalyst; the urea is a first-class product for agriculture, and the N content of the urea is more than or equal to 46.2 percent; the potassium nitrate is agricultural superior product, and K thereof2O content is more than or equal to 46 percent, H2O≤0.5%。
Further, the preparation method of the plant source disease-resistant growth promoter for resisting wheat root rot comprises the following main steps:
(1) carrying out chelation reaction: adding various raw materials into an automatic feeding device, sequentially adding wood vinegar, potassium humate, urea and potassium nitrate into a reaction kettle in sequence, stirring and dissolving, carrying out chelation reaction for 0.5-1 h, and cooling to room temperature to obtain a mixed solution A;
(2) sanding: further sanding and stirring the mixed solution A obtained in the step (1) through a sanding machine to enable the mixed solution to be uniform, and obtaining a mixed solution B;
(3) and (3) filtering: filtering the mixed solution B obtained in the step (2) by a filter;
(4) filling: and packaging by an automatic filling machine to obtain the plant source disease-resistant growth promoter for resisting the wheat root rot.
Further, the reaction conditions of the chelation reaction in the step (1) are as follows: heating to 60-80 deg.C, and adjusting pH to 4.5-6.5.
Furthermore, the plant-derived disease-resistant growth promoter for resisting wheat root rot is applied to prevention or treatment of wheat root rot.
Has the advantages that:
(1) the invention has strong commodity and applicability and directly guides agricultural production
The invention takes the wood vinegar as the main component, adds humic acid and nitrogen and potassium nutrient elements to prepare the humic acid-containing water-soluble fertilizer, sets a corresponding application method, can directly guide agricultural production, and solves the problem of the last kilometer of the product. Meanwhile, the disease-resistant substance is prepared by pyrolyzing and gasifying leftovers of agricultural and forestry plants, takes wood vinegar as a main component, is a new water-soluble fertilizer raw material, and realizes resource utilization of agricultural wastes. The adjustment of the extraction process parameters of the pyroligneous liquor is directly carried out on a production line, and the defect that the parameters of small tests and industrialized production are inconsistent can be avoided.
(2) The product of the invention has strong function and stable property
The improved biomass gasification producer furnace disclosed by the application publication No. CN 109694749A is adopted in the production process, the biomass gasification producer furnace is good in airtightness, gas does not leak in the feeding and air supplying processes, materials are uniformly dropped in the furnace body, the reaction is complete, the combustion effect of combustible gas is good, and therefore the produced pyroligneous liquor is stable in property. The content of acetic acid which is the main component of the pyroligneous liquor is proved to be 9.4 percent, which is improved by 6.4 percent compared with the conventional process (the content of the acetic acid in the pyroligneous liquor is about 3 percent generally). Research shows that the size of the bacteriostatic ability of the wood vinegar liquid is related to the content of the organic acid contained in the wood vinegar liquid, and after the crude wood vinegar liquid is refined, the content of the organic acid in the wood vinegar liquid is reduced, and the bacteriostatic ability of the wood vinegar liquid is correspondingly reduced. In the invention, the content of acetic acid is increased, so that the antibacterial capability of the antibacterial agent is enhanced.
(3) The invention contains humic acid which has the functions of promoting root growth and improving drought resistance of crops. Therefore, the product of the invention has multiple functions of growth promotion, stress resistance and the like, has strong multifunctional effect stability, and meets the requirement of the development trend of diversified functions and stable effect of the water-soluble fertilizer in the future.
Detailed Description
In order to make the technical solutions in the present application better understood, the present invention is further described below with reference to examples, which are only a part of examples of the present application, but not all examples, and the present invention is not limited by the following examples.
Example 1 inhibitory Effect of Wood Vinegar on pathogenic bacteria and influence of wheat Germination
1 materials and methods
1.1 test materials: root rot pathogens, fusarium (fusarium graminearum) and helminthosporium (bipolarisrokiniana), placed in PDA medium for standby; the tested wheat variety is Jimai 22; the wood vinegar is the stock solution developed by the invention.
1.2 test methods:
1.2.1 inhibition of hypha growth of Rhizopus tritici by Wood Vinegar
The PDA culture medium is sterilized and mixed with pyroligneous liquor according to the proportion of 0.02%, 0.05%, 0.1%, 0.20%, 0.25%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1.0%, 1.5% and 2.0%, and the mixture is poured into a 9cm flat dish, and each concentration is poured into 3 dishes, and each dish is poured into 20 mL. 2 wheat root rot pathogenic bacteria are placed in a PDA culture medium for culturing for 10 days, 2 fungus cakes (with the diameter of 5mm) are punched on a test fungus plate by a puncher and placed on a plate culture medium containing pyroligneous liquor, the culture medium with each concentration and a Control (CK) culture medium are respectively placed in 3 dishes and are placed in an incubator at 28 ℃ for culturing for 3 days, and the diameters of the colonies are measured. The inhibition rate of each treatment on the growth of hyphae is calculated according to the following formula: inhibition hypha growth rate (%) [ (control colony diameter-treated colony diameter)/control colony diameter ] × 100%
1.2.2 Effect of disease-resistant growth-promoting Agents on wheat Germination
The pyroligneous acid concentration is set to 0.05%, 0.1%, 0.15%, 0.20%, 0.25%, 0.3%, 0.4%, 0.5%, 0.8%, 1.0%, 1.5%, and 2.0%. Germination tests were performed in petri dishes, 50 soaked seeds were placed in each dish, 10ml of each different concentration of culture medium was added, and 5 replicates of each treatment were set up, using distilled water treatment as a control. Placing the culture dish into an incubator, wherein the temperature is 24 ℃, and the light/dark: 16h/8 h. The number of sprouts on days 3 and 7 was recorded, and the length of the root and aerial parts of the sprouts on day 7 was measured. The germination potential and the germination rate were calculated according to the following formulas:
germination vigor (%) - (number of normal germination grains/number of test seeds in 3 days) × 100%
The germination percentage (%) - (number of normal germination grains/number of test seeds in 7 days) × 100%
2 results and analysis
2.1 Effect of Wood Vinegar on growth of Helminthosporium hyphae
The hyphae of the helminthosporium (bipolaris sorokiniana) do not grow on a pyroligneous liquor-PDA culture medium with the concentration of more than or equal to 0.8 percent, and the inhibition rate is 100 percent; hyphae can grow on a pyroligneous liquor-PDA culture medium with the concentration of 0.4%, 0.5% and 0.6%, the inhibition rates are 81.56%, 87.78% and 89.31%, and the difference among the three is not obvious; when the concentration is less than or equal to 0.3%, the inhibition rate of the pyroligneous on the growth of the hyphae of the helminthosporium is less than 50%, and the difference among treatments is obvious (table 1).
TABLE 1 inhibition of Helminthosporium hyphae growth by different concentrations of disease-resistant growth-promoting agent
Figure BDA0002292905460000041
2.2 Effect of Wood Vinegar on Fusarium hypha growth
When the concentration of the wood vinegar is more than or equal to 0.8%, the hyphae of fusarium graminearum do not grow, and the inhibition rate is 100%; when the concentration is 0.2-0.6%, hyphae can grow, and the inhibition rate is 50.03-83.78%; at concentrations < 0.2%, the inhibition of fusarium hyphae growth by pyroligneous liquor was < 50% (table 2).
TABLE 2 inhibition of Fusarium mycelial growth by different concentrations of pyroligneous liquor
Figure BDA0002292905460000042
Figure BDA0002292905460000051
2.3 Effect of Wood Vinegar on wheat Germination and growth
When the concentration of the wood vinegar is less than or equal to 0.50 percent, the germination vigor and the germination rate of the wheat are not influenced; at concentrations > 0.50%, both the germination vigour and the germination rate of wheat were affected to different extents and both the germination vigour and the germination rate decreased significantly with increasing pyroligneous liquor concentration (table 3).
When the concentration of the wood vinegar is less than or equal to 1.00 percent, the growth of the overground part and the underground part of the wheat is not obviously influenced; at concentrations > 1.50%, the growth of wheat was affected to different extents both above-ground and below-ground (Table 3).
TABLE 3 influence of different concentrations of pyroligneous acid on germination and growth of wheat
Figure BDA0002292905460000052
3 conclusion
The research proves that the pyroligneous liquor with the concentration of 0.6 percent has obvious inhibition effect on the pathogenic bacteria of the wheat root rot and does not influence the growth of wheat. Therefore, the concentration of the 0.6 percent wood vinegar is the optimal concentration for inhibiting the wheat root rot.
Example 2 preparation of a plant-derived disease-resistant growth promoter for resisting wheat root rot and application thereof
1. The plant source disease-resistant growth promoter for resisting wheat root rot comprises the following raw material components in parts by weight:
55.5 parts of pyroligneous; 6.5 parts of potassium fulvate; 19 parts of urea; 19 parts of potassium nitrate.
Meets the standards of humic acid-containing water-soluble fertilizers.
2. A plant source disease-resistant growth promoter for resisting wheat root rot is prepared by the following main steps:
(1) carrying out chelation reaction: adding various raw materials into an automatic feeding device, sequentially adding the raw materials into a reaction kettle according to the characteristics of the raw materials, firstly adding wood vinegar, then adding potassium humate, stirring for dissolving, finally adding urea and potassium nitrate, heating to 70 ℃, adjusting the pH value to 5.5, carrying out chelation reaction, and reacting for 1 h. After the chelation reaction is finished, cooling to room temperature.
(2) Sanding: and further sanding and stirring the mixed solution through a sand mill to ensure that the mixed solution is uniform.
(3) And (3) filtering: filtering the uniformly mixed solution through a filter.
(4) Filling: and packaging by an automatic filling machine to obtain the plant source disease-resistant growth promoter product for resisting the wheat root rot.
The automatic feeding device is of a YBF type; the reaction kettle is FUJS-5000A type; the sand mill is an SX series vertical sand mill; the filter is of a GPS-1200 type and adopts a high-frequency vibrating screen; the filling machine is of a CZP-16G type.
3. A field high-efficiency application method of a plant source disease-resistant growth promoter for resisting wheat root rot comprises the following steps:
the formula is suitable for plots with light wheat root rot. In 3-5 months, the product prepared in example 2 is diluted by 100 times, the dosage is 5 square/mu/time, the interval time is 15 days, and the times are 2-3 times.
Example 3 preparation of a plant-derived disease-resistant growth promoter for resisting wheat root rot and application thereof
1. The plant source disease-resistant growth promoter for resisting wheat root rot comprises the following raw material components in parts by weight:
80 parts of pyroligneous; 9 parts of potassium fulvate; 8 parts of urea; 3 parts of potassium nitrate.
2. A preparation method of a plant source disease-resistant growth promoter for resisting wheat root rot comprises the following steps:
(1) carrying out chelation reaction: adding various raw materials into an automatic feeding device, sequentially adding the raw materials into a reaction kettle according to the characteristics of the raw materials, firstly adding wood vinegar, then adding potassium humate, stirring for dissolving, finally adding urea and potassium nitrate, heating to 80 ℃, adjusting the pH value to 5.5, carrying out chelation reaction, and reacting for 1 h. After the chelation reaction is finished, cooling to room temperature.
(2) Sanding: and further sanding and stirring the mixed solution through a sand mill to ensure that the mixed solution is uniform.
(3) And (3) filtering: filtering the uniformly mixed solution through a filter.
(4) Filling: and packaging by an automatic filling machine to obtain the plant source disease-resistant growth promoter product for resisting the wheat root rot.
The automatic feeding device is of a YBF type; the reaction kettle is FUJS-5000A type; the sand mill is an SX series vertical sand mill; the filter is of a GPS-1200 type and adopts a high-frequency vibrating screen; the filling machine is of a CZP-16G type.
3. A field high-efficiency application method of a plant source disease-resistant growth promoter for resisting wheat root rot comprises the following steps:
the formula is suitable for plots with severe wheat root rot. When the wheat has root rot symptoms, the product of the embodiment 3 is diluted by 100 times, the using amount is 5 square/mu/time, the root irrigation is carried out at intervals of 10-15 days, and the root irrigation is carried out for 2-5 times according to the disease conditions.
Example 4 therapeutic Effect of a plant-derived disease-resistant growth promoter against wheat root rot
1 materials and methods
1.1 test materials: the wheat variety is Jimai No. 22. The variety of the water-soluble fertilizer to be tested is humic acid-containing water-soluble fertilizer (purchased in the market); a special water-soluble fertilizer for wheat (purchased in market); wood vinegar; an anti-disease growth promoter (prepared as in example 3).
1.2 experimental design: a pot experiment method is adopted, and 5 treatments are set, namely (1) CK (no fertilization) respectively; (2) treatment 1 (containing humic acid water-soluble fertilizer); (3) treatment 2 (water-soluble fertilizer special for wheat); (4) treatment 3 (pyroligneous liquor); (5) treatment 4 (anti-growth promoting agent), 3 replicates per treatment. The following table 4 is specific:
table 4 experimental design
Figure BDA0002292905460000071
1.3 test methods: taking surface soil of field wheat-peanut rotation, sieving by a 2mm sieve, and performing rotation according to the following steps of: adding formaldehyde into soil at a ratio of 1:500(V/w), turning over, covering with plastic film, and sterilizing for 5 days. The application amount of the base fertilizer is 6.4g of urea per pot, 3.9g of triple superphosphate per pot and 2.5g of potassium sulfate per pot. And (4) uniformly mixing the base fertilizer and the sterilized soil, and filling the mixture into pots, wherein 14kg of soil is filled in each pot. Watering each pot for about 2 liters, sowing wheat after the surface soil is slightly dry, uniformly distributing the wheat grains in 20 grains/pot, and then covering a layer of dry soil. The method comprises the following steps of (1) propagating rhizoctonia solani and helminthosporium pathogenic bacteria by wheat grains, and inoculating the rhizoctonia solani and helminthosporium pathogenic bacteria to wheat root soil according to a proportion of 3% (w/w) in the booting stage of wheat, wherein the specific method comprises the following steps: controlling the water content of the wheat soil to be 60-70%, carefully digging out the soil with the depth of 5cm around the root of the wheat (exposing the root hair of the wheat), respectively mixing the soil with the wheat grains full of two root rot pathogenic bacteria, and backfilling the mixture into a pot. In order to prevent the direct irradiation of sunlight from influencing the growth of pathogenic bacteria, black plastic bags are used for covering the bacteria-receiving soil. After one week, root irrigation treatment is carried out according to the treatment, and after 7d, secondary treatment is carried out.
1.4 survey and determination method: during the mature period of wheat, the roots of each treated wheat are taken, and the disease condition, the dry weight of overground parts, the dry weight of roots, the plant height and the yield of the root rot of each treated wheat are investigated.
2 results and analysis
TABLE 5 control of wheat root rot by different treatments
Figure BDA0002292905460000081
As shown in Table 5, the root irrigation treatment of the water-soluble fertilizer, the wood vinegar and the disease-resistant growth promoter reduces the disease index of the root rot of wheat and improves the prevention and treatment effect, wherein the disease-resistant growth promoter has the best inhibition effect on pathogenic bacteria, and the inhibition rate reaches 46.60%.
TABLE 6 Effect of different treatments on wheat growth and yield
From table 6, the water-soluble fertilizer containing humic acid, the special water-soluble fertilizer for wheat and the wood vinegar are higher than the control in the aspects of ground dry weight, root dry weight, thousand grain weight and yield, but the difference does not reach a significant level; the dry weight, dry root weight, thousand kernel weight and yield of the disease-resistant growth promoter treated ground are all obviously higher than those of a control. The water soluble fertilizer, the wood vinegar and the disease-resistant growth promoter promote the growth of wheat to a certain extent, but the disease-resistant growth promoter has obvious influence on the effect.
3 conclusion
The research shows that the disease index of the wheat root rot can be obviously reduced by root irrigation of the disease-resistant growth promoter, and the inhibition rate reaches 46.60%; promoting the growth of wheat and increasing the yield.
Example 5 field high efficiency application method and effect of wheat root rot resistant plant source disease resistant growth promoter
1 materials and methods
1.1 test materials: the wheat variety is Jimai No. 22. The variety of the water-soluble fertilizer to be tested is humic acid-containing water-soluble fertilizer (purchased in the market); a special water-soluble fertilizer for wheat (purchased in market); wood vinegar; an anti-disease growth promoter (prepared as in example 2).
1.2 experimental design: the experiment was carried out with 6 treatments, each of which was repeated 3 times, and the cell area was 30m2Random block permutation. Respectively as follows: (1) t1: control, clear water; (2) t2: diluting a commercially available humic acid-containing water-soluble fertilizer by 500 times; (3) t3: diluting the special water-soluble fertilizer for wheat sold in the market by 500 times; (4) t4: diluting the wood vinegar stock solution by 300 times; (5) t5: the disease-resistant growth promoter is developed by the invention, the dilution factor is 100 times, and the application frequency is 1 time; (6) t6: the disease-resistant growth promoter is developed by the invention, the dilution factor is 100 times, and the application frequency is 2 times.
1.3 test methods: and 3-6 months in 2019, and the research on the application effect of the disease-resistant growth-promoting agent developed in the village wheat field of Daqiaotown Yao shops in Jinan city is shown. T6 is processed in the turning green and heading stage and the booting stage, and the other is processed in the turning green and heading stage, diluted according to the specification, the final uniform dosage is 5 square/mu/time, and then the solution or water is uniformly irrigated to the root of the wheat.
1.4 survey measurement items: the wheat mature period is respectively investigated the disease condition of the root rot of each treated wheat, the yield of the wheat is measured after the wheat is harvested, and a soil sample (0-20cm) is taken to measure pH, organic matters, alkaline hydrolysis nitrogen, available phosphorus and available potassium.
2 results and analysis
TABLE 7 control of wheat root rot by different treatments
Figure BDA0002292905460000091
The results in Table 7 show that the water-soluble fertilizer, the wood vinegar and the disease-resistant growth promoter are used for root irrigation, the morbidity degree of the wheat root rot is remarkably reduced, the prevention effect is 21.46-38.14%, and the prevention effect is the best when the disease-resistant growth promoter is applied for 2 times.
TABLE 8 Effect of different treatments on wheat yield and soil Properties
Figure BDA0002292905460000092
Figure BDA0002292905460000101
As can be seen from the table 8, the yield of wheat treated by the disease-resistant growth promoter for 2 times is the highest, which is obviously higher than that of wheat treated by other treatments, and the yield is increased by 5.3% -6.1% compared with that of the humic acid-containing and wheat-dedicated water-soluble fertilizer.
The disease-resistant growth promoter applied for 2 times obviously reduces the pH value of the soil, is possibly related to the condition that wood vinegar in raw materials is acidic and the application amount is large, but has no fundamental influence on the acidity and the alkalinity of the soil. Compared with the common water soluble fertilizer sold in the market, the disease-resistant growth promoter applied for 2 times obviously improves the content of organic matters, alkaline hydrolysis nitrogen and available phosphorus in the soil.
3 conclusion
In conclusion, the fertilizing method of uniformly irrigating the disease-resistant growth promoter to the root of the wheat for 2 times after diluting the disease-resistant growth promoter by 100 times with clear water has the best effect on preventing the root rot of the wheat, increasing the yield of the wheat and fertilizing the soil.

Claims (5)

1. A plant source disease-resistant growth promoter for resisting wheat root rot is characterized by comprising the following raw materials in parts by weight:
55-80 parts of pyroligneous, 6-9 parts of potassium fulvate, 8-19 parts of urea and 3-19 parts of potassium nitrate.
2. The plant-derived disease-resistant growth-promoting agent according to claim 1, wherein the preparation method of the wood vinegar solution comprises: the wood chips and the cotton straws are put into a biomass gasification generating furnace according to the mass ratio of 1-2:1, are subjected to vaporization treatment at the temperature of 740-.
3. The method for preparing a plant-derived anti-pathogenic growth promoter according to any one of claims 1 to 2, comprising the steps of:
(1) carrying out chelation reaction: sequentially adding the pyroligneous liquor, the potassium humate, the urea and the potassium nitrate into a reaction kettle in sequence, stirring and dissolving, carrying out chelation reaction for 0.5-1 h, and cooling to room temperature to obtain a mixed solution A;
(2) sanding: further sanding and stirring the mixed solution A obtained in the step (1) through a sanding machine to enable the mixed solution to be uniform, and obtaining a mixed solution B;
(3) and (3) filtering: filtering the mixed solution B obtained in the step (2) by a filter;
(4) filling: and packaging by an automatic filling machine to obtain the plant source disease-resistant growth promoter for resisting the wheat root rot.
4. The method for preparing a plant-derived disease-resistant growth-promoting agent according to claim 3, wherein the reaction conditions of the chelation reaction in the step (1) are as follows: heating to 60-80 deg.C, and adjusting pH to 4.5-6.5.
5. The use of a plant-derived disease resistance promoter against wheat root rot according to any one of claims 1 to 2 for the prevention or treatment of wheat root rot.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111592405A (en) * 2020-05-29 2020-08-28 山东省农业科学院农业资源与环境研究所 Organic water-soluble fertilizer capable of resisting wheat dry hot air and powdery mildew as well as preparation and application thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111592405A (en) * 2020-05-29 2020-08-28 山东省农业科学院农业资源与环境研究所 Organic water-soluble fertilizer capable of resisting wheat dry hot air and powdery mildew as well as preparation and application thereof
CN111592405B (en) * 2020-05-29 2021-11-09 山东省农业科学院农业资源与环境研究所 Organic water-soluble fertilizer capable of resisting wheat dry hot air and powdery mildew as well as preparation and application thereof

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