CN110663502A - Drought-resistant matrix for cultivating alfalfa - Google Patents
Drought-resistant matrix for cultivating alfalfa Download PDFInfo
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- CN110663502A CN110663502A CN201911158454.0A CN201911158454A CN110663502A CN 110663502 A CN110663502 A CN 110663502A CN 201911158454 A CN201911158454 A CN 201911158454A CN 110663502 A CN110663502 A CN 110663502A
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- alfalfa
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- 241000219823 Medicago Species 0.000 title claims abstract description 47
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 title claims abstract description 47
- 239000011159 matrix material Substances 0.000 title claims abstract description 12
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 26
- 235000021190 leftovers Nutrition 0.000 claims abstract description 26
- 239000003245 coal Substances 0.000 claims abstract description 23
- 239000002689 soil Substances 0.000 claims abstract description 22
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 18
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 18
- 150000004676 glycans Chemical class 0.000 claims abstract description 18
- 229920002643 polyglutamic acid Polymers 0.000 claims abstract description 18
- 229920001184 polypeptide Polymers 0.000 claims abstract description 18
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 18
- 239000005017 polysaccharide Substances 0.000 claims abstract description 18
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 18
- 239000011591 potassium Substances 0.000 claims abstract description 18
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 18
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 18
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 18
- 230000002745 absorbent Effects 0.000 claims abstract description 17
- 239000002250 absorbent Substances 0.000 claims abstract description 17
- 239000000835 fiber Substances 0.000 claims abstract description 17
- 239000000411 inducer Substances 0.000 claims abstract description 17
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 239000000758 substrate Substances 0.000 claims description 19
- 238000003756 stirring Methods 0.000 claims description 5
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical group C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 2
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 2
- 239000006013 carbendazim Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000004563 wettable powder Substances 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 10
- 239000002028 Biomass Substances 0.000 abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 8
- 230000008641 drought stress Effects 0.000 abstract description 4
- 208000035240 Disease Resistance Diseases 0.000 abstract description 3
- 230000035882 stress Effects 0.000 abstract description 3
- 230000001965 increasing effect Effects 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000004459 forage Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010042209 Stress Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000004918 root sheath Anatomy 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/17—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing slag
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a drought-resistant matrix for cultivating alfalfa, which is prepared from the following raw materials: sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer, mushroom leftovers and graft modified soybean protein. The invention can enhance the disease resistance and stress resistance of the alfalfa, can ensure that the alfalfa can still maintain the phenotype of a healthy plant under drought stress, enhances the water retention performance around the alfalfa rhizosphere, and has higher biomass and water content of the overground part.
Description
Technical Field
The invention relates to the field of pasture planting, in particular to a drought-resistant matrix for cultivating alfalfa.
Background
The alfalfa is perennial leguminous forage widely cultivated in semiarid regions in China, has the characteristics of high yield, high quality and strong adaptability, is qualified as the king of the forage, however, as the alfalfa belongs to deep-rooted plants, has strong water consumption on soil, and commonly occurs in soil desiccation, the production of the alfalfa is gradually slowed down, the yield is continuously reduced, the grassland is degraded, the feed supply of the animal husbandry is obstructed, and the development of the grass industry and the animal husbandry is restricted.
Although screening of drought-resistant alfalfa varieties becomes an important way for improving drought resistance of alfalfa, developed root systems are often used as a main screening index for strong drought resistance of alfalfa. However, the developed root system will further enhance the water consumption of alfalfa, aggravate the soil drought and further cause the ecological environment problem of soil moisture. Therefore, from the point of view of long-term production of alfalfa and environmental sustainability, the matching research of the culture medium is carried out, and the method for improving the drought resistance of alfalfa by improving the microenvironment for growth of alfalfa, enhancing the water retention performance around the alfalfa rhizosphere and improving the drought resistance of alfalfa is bound to become a simple, efficient and sustainable approach.
Disclosure of Invention
In order to solve the problems, the invention provides a drought-resistant matrix for cultivating alfalfa, which can enhance the disease resistance and stress tolerance of alfalfa.
In order to achieve the purpose, the invention adopts the technical scheme that:
a drought-resistant matrix for cultivating alfalfa is prepared from the following raw materials: sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer, mushroom leftovers and graft modified soybean protein.
Preferably, the drought-resistant matrix is prepared from the following raw materials in parts by weight:
20-40 parts of sandy soil, 10-15 parts of weathered coal, 1.2-1.5 parts of polysaccharide polypeptide biological potassium, 1-3 parts of gamma-polyglutamic acid, 3-6 parts of degradable super absorbent fibers, 0.1-0.3 part of photosynthetic inducer, 10-20 parts of mushroom leftovers and 10-15 parts of graft modified soybean protein.
Preferably, the drought-resistant matrix is prepared from the following raw materials in parts by weight:
20 parts of sandy soil, 10 parts of weathered coal, 1.2 parts of polysaccharide polypeptide biological potassium, 1 part of gamma-polyglutamic acid, 3 parts of degradable super absorbent fiber, 0.1 part of photosynthetic inducer, 10 parts of mushroom leftovers and 10 parts of graft modified soybean protein.
Preferably, the drought-resistant matrix is prepared from the following raw materials in parts by weight:
40 parts of sandy soil, 15 parts of weathered coal, 1.5 parts of polysaccharide polypeptide biological potassium, 3 parts of gamma-polyglutamic acid, 6 parts of degradable super absorbent fiber, 0.3 part of photosynthetic inducer, 20 parts of mushroom leftovers and 15 parts of graft modified soybean protein.
Preferably, the drought-resistant matrix is prepared from the following raw materials in parts by weight:
30 parts of sandy soil, 12.5 parts of weathered coal, 1.35 parts of polysaccharide polypeptide biological potassium, 2 parts of gamma-polyglutamic acid, 4.5 parts of degradable super absorbent fiber, 0.2 part of photosynthetic inducer, 15 parts of mushroom leftovers and 12.5 parts of graft modified soybean protein.
Further, the mushroom leftovers are obtained by the following steps:
after the recycled mushroom leftovers are tidied and concentrated, 50% carbendazim wettable powder 600-time liquid is sprayed while stirring, and after uniform mixing, the mushroom leftovers are piled, stuffy and decomposed for 2-3 months.
The invention has the following beneficial effects:
the method can enhance the disease resistance and stress resistance of the alfalfa, and simultaneously enhance the water retention performance around the alfalfa rhizosphere, so that the alfalfa can still maintain the phenotype of a healthy plant under drought stress, and has higher biomass and water content of the overground part.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
S1, weighing the following components in parts by weight: 40 parts of sandy soil, 15 parts of weathered coal, 1.5 parts of polysaccharide polypeptide biological potassium, 3 parts of gamma-polyglutamic acid, 6 parts of degradable super absorbent fiber, 0.3 part of photosynthetic inducer, 20 parts of mushroom leftovers and 15 parts of graft modified soybean protein;
s2, crushing the weighed weathered coal and mushroom leftovers, and mixing and stirring the crushed weathered coal and mushroom leftovers with sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer and graft modified soybean protein uniformly to obtain the fertilizer.
Example 2
S1, weighing the following components in parts by weight: 40 parts of sandy soil, 15 parts of weathered coal, 1.5 parts of polysaccharide polypeptide biological potassium, 3 parts of gamma-polyglutamic acid, 6 parts of degradable super absorbent fiber, 0.3 part of photosynthetic inducer, 20 parts of mushroom leftovers and 15 parts of graft modified soybean protein;
s2, crushing the weighed weathered coal and mushroom leftovers, and mixing and stirring the crushed weathered coal and mushroom leftovers with sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer and graft modified soybean protein uniformly to obtain the fertilizer.
Example 3
S1, weighing the following components in parts by weight: 30 parts of sandy soil, 12.5 parts of weathered coal, 1.35 parts of polysaccharide polypeptide biological potassium, 2 parts of gamma-polyglutamic acid, 4.5 parts of degradable super absorbent fiber, 0.2 part of photosynthetic inducer, 15 parts of mushroom leftovers and 12.5 parts of graft modified soybean protein;
s2, crushing the weighed weathered coal and mushroom leftovers, and mixing and stirring the crushed weathered coal and mushroom leftovers with sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer and graft modified soybean protein uniformly to obtain the fertilizer.
Examples of the experiments
Experimental group 1: the substrate obtained in example 1;
experimental group 2: the substrate obtained in example 2;
experimental group 3: the substrate obtained in example 3;
control group 1: sandy soil;
control group 2: the polysaccharide polypeptide biological potassium is not contained, and the rest is the same as the embodiment 3;
control group 3: the same as example 3 except that gamma-polyglutamic acid was not contained;
control group 4: the same procedure as in example 3 was repeated except that the graft-modified soybean protein was not contained.
The experimental method comprises the following steps:
transferring alfalfa seedlings with radicles about 2cm long to plastic pots filled with different culture medium formulas for growth, transplanting 12 seedlings in each pot, and after one week of seedling revival, fixedly planting the number of seedlings in each pot into 8 seedlings. Watering twice every week, stopping watering after the alfalfa grows for 1 month, carrying out drought treatment, and collecting samples after the drought treatment for one month. The experiment was repeated three times and the results averaged.
1. Plant phenotype
The phenotype of the plants subjected to drought treatment for one month in different substrate formulas is observed, the leaves of the alfalfa in the control group 1 are severely wilted, typical drought stress symptoms appear, most (more than 60%) of the leaves in the control group 2 are severely wilted, a small part (less than 40%) of the leaves in the control group 3 are severely wilted, and a small part (less than 40%) of the leaves in the control group 4 are severely wilted. The alfalfa in the experimental group 1, the experimental group 2 and the experimental group 3 has stronger drought resistance, and leaves do not have wilting phenomenon, which shows that the substrate is favorable for the growth of the alfalfa under the drought condition.
2. Root system phenotype
The underground part of the plant was harvested and observed for root phenotype after one month of drought stress treatment in different medium formulations.
The alfalfa root system of the control group 1 is small, no adsorption matrix is arranged on the surface of the root system, the control group 2 is the third control group 3, and the fourth control group 4; the alfalfa in the experimental group 1, the experimental group 2 and the experimental group 3 has a large root system, and a large amount of wet substrates are adsorbed on the surface of the root system to form a root sheath, so that the water retention capacity around the rhizosphere is enhanced, and the drought resistance of the alfalfa is improved.
3. Comparison of Biomass
The phenotype of the plants grown drought for one month in the different substrate formulations was observed and the overground and underground biomass of the plants (referred to as the overground and underground dry weight) was measured by harvesting the plants. The biomass of the overground part and the underground part of the alfalfa in the control group 1 is the minimum, the biomass of the alfalfa in the control group 2 is the second, the control group 3 is the third, and the control group 4 is the fourth; the biomass of the aerial parts and the underground parts of the alfalfa in the experimental group 1, the experimental group 2 and the experimental group 3 is increased, and the biomass of the aerial parts is the largest in the example 3, which shows that the substrate provided by the invention is beneficial to increasing the yield of the alfalfa under the drought condition.
4. Water content of overground part
Harvesting alfalfa growing in different substrate formulations for one month under drought, weighing the fresh weight of the overground part, deactivating enzymes in an oven at 105 ℃ for half an hour, drying the plant material at 70 ℃ for at least 2 days, and measuring the dry weight of the overground part. The water content of the above-ground part was calculated from the difference between the fresh weight and the dry weight.
The water content of the above-ground parts of the alfalfa in the control group was small, the second control group 2, the third control group 3 and the fourth control group 4; the water content of the upper part of the alfalfa field of the experimental group 1, the experimental group 2 and the experimental group 3 is obviously increased, which shows that the substrate is beneficial to increasing the drought resistance of the alfalfa.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (6)
1. A drought-resistant matrix for cultivating alfalfa is characterized by being prepared from the following raw materials: sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer, mushroom leftovers and graft modified soybean protein.
2. The drought-resistant substrate for cultivating alfalfa as claimed in claim 1, wherein the drought-resistant substrate is prepared from the following raw materials in parts by weight:
20-40 parts of sandy soil, 10-15 parts of weathered coal, 1.2-1.5 parts of polysaccharide polypeptide biological potassium, 1-3 parts of gamma-polyglutamic acid, 3-6 parts of degradable super absorbent fibers, 0.1-0.3 part of photosynthetic inducer, 10-20 parts of mushroom leftovers and 10-15 parts of graft modified soybean protein.
3. The drought-resistant substrate for cultivating alfalfa as claimed in claim 1, wherein the drought-resistant substrate is prepared from the following raw materials in parts by weight:
20 parts of sandy soil, 10 parts of weathered coal, 1.2 parts of polysaccharide polypeptide biological potassium, 1 part of gamma-polyglutamic acid, 3 parts of degradable super absorbent fiber, 0.1 part of photosynthetic inducer, 10 parts of mushroom leftovers and 10 parts of graft modified soybean protein.
4. The drought-resistant substrate for cultivating alfalfa as claimed in claim 1, wherein the drought-resistant substrate is prepared from the following raw materials in parts by weight:
40 parts of sandy soil, 15 parts of weathered coal, 1.5 parts of polysaccharide polypeptide biological potassium, 3 parts of gamma-polyglutamic acid, 6 parts of degradable super absorbent fiber, 0.3 part of photosynthetic inducer, 20 parts of mushroom leftovers and 15 parts of graft modified soybean protein.
5. The drought-resistant substrate for cultivating alfalfa as claimed in claim 1, wherein the drought-resistant substrate is prepared from the following raw materials in parts by weight:
30 parts of sandy soil, 12.5 parts of weathered coal, 1.35 parts of polysaccharide polypeptide biological potassium, 2 parts of gamma-polyglutamic acid, 4.5 parts of degradable super absorbent fiber, 0.2 part of photosynthetic inducer, 15 parts of mushroom leftovers and 12.5 parts of graft modified soybean protein.
6. The drought-resistant substrate for cultivating alfalfa as claimed in any of claims 1-5, wherein the mushroom leftover is obtained by the following steps:
after the recycled mushroom leftovers are tidied and concentrated, 50% carbendazim wettable powder 600-time liquid is sprayed while stirring, and after uniform mixing, the mushroom leftovers are piled, stuffy and decomposed for 2-3 months.
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Cited By (2)
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CN113396682A (en) * | 2021-07-28 | 2021-09-17 | 山西农业大学高寒区作物研究所(山西省农业科学院高寒区作物研究所) | Fertilizing method for remarkably improving yield of alfalfa |
CN113443944A (en) * | 2021-08-02 | 2021-09-28 | 山西农业大学高寒区作物研究所(山西省农业科学院高寒区作物研究所) | Special organic compound fertilizer for alfalfa |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN113396682A (en) * | 2021-07-28 | 2021-09-17 | 山西农业大学高寒区作物研究所(山西省农业科学院高寒区作物研究所) | Fertilizing method for remarkably improving yield of alfalfa |
CN113443944A (en) * | 2021-08-02 | 2021-09-28 | 山西农业大学高寒区作物研究所(山西省农业科学院高寒区作物研究所) | Special organic compound fertilizer for alfalfa |
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