CN110663502B - Drought-resistant matrix for cultivating alfalfa - Google Patents
Drought-resistant matrix for cultivating alfalfa Download PDFInfo
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- CN110663502B CN110663502B CN201911158454.0A CN201911158454A CN110663502B CN 110663502 B CN110663502 B CN 110663502B CN 201911158454 A CN201911158454 A CN 201911158454A CN 110663502 B CN110663502 B CN 110663502B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/17—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing slag
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a drought-resistant matrix for cultivating alfalfa, which is prepared from the following raw materials: sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer, mushroom leftovers and graft modified soybean protein. The invention can enhance the disease resistance and stress resistance of the alfalfa, can ensure that the alfalfa can still maintain the phenotype of a healthy plant under drought stress, enhances the water retention performance around the alfalfa rhizosphere, and has higher biomass and water content of the overground part.
Description
Technical Field
The invention relates to the field of pasture planting, in particular to a drought-resistant matrix for cultivating alfalfa.
Background
The alfalfa is perennial leguminous forage widely cultivated in semiarid regions in China, has the characteristics of high yield, high quality and strong adaptability, is qualified as the king of the forage, however, as the alfalfa belongs to deep-rooted plants, has strong water consumption on soil, and commonly occurs in soil desiccation, the production of the alfalfa is gradually slowed down, the yield is continuously reduced, the grassland is degraded, the feed supply of the animal husbandry is obstructed, and the development of the grass industry and the animal husbandry is restricted.
Although screening of drought-resistant alfalfa varieties becomes an important way for improving drought resistance of alfalfa, developed root systems are often used as a main screening index for strong drought resistance of alfalfa. However, the developed root system will further enhance the water consumption of alfalfa, aggravate the soil drought and further cause the ecological environment problem of soil moisture. Therefore, from the point of view of long-term production of alfalfa and environmental sustainability, the matching research of the culture medium is carried out, and the method for improving the drought resistance of alfalfa by improving the microenvironment for growth of alfalfa, enhancing the water retention performance around the alfalfa rhizosphere and improving the drought resistance of alfalfa is bound to become a simple, efficient and sustainable approach.
Disclosure of Invention
In order to solve the problems, the invention provides a drought-resistant matrix for cultivating alfalfa, which can enhance the disease resistance and stress tolerance of alfalfa.
In order to achieve the purpose, the invention adopts the technical scheme that:
a drought-resistant matrix for cultivating alfalfa is prepared from the following raw materials: sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer, mushroom leftovers and graft modified soybean protein.
Preferably, the drought-resistant matrix is prepared from the following raw materials in parts by weight:
20-40 parts of sandy soil, 10-15 parts of weathered coal, 1.2-1.5 parts of polysaccharide polypeptide biological potassium, 1-3 parts of gamma-polyglutamic acid, 3-6 parts of degradable super absorbent fibers, 0.1-0.3 part of photosynthetic inducer, 10-20 parts of mushroom leftovers and 10-15 parts of graft modified soybean protein.
Preferably, the drought-resistant matrix is prepared from the following raw materials in parts by weight:
20 parts of sandy soil, 10 parts of weathered coal, 1.2 parts of polysaccharide polypeptide biological potassium, 1 part of gamma-polyglutamic acid, 3 parts of degradable super absorbent fiber, 0.1 part of photosynthetic inducer, 10 parts of mushroom leftovers and 10 parts of graft modified soybean protein.
Preferably, the drought-resistant matrix is prepared from the following raw materials in parts by weight:
40 parts of sandy soil, 15 parts of weathered coal, 1.5 parts of polysaccharide polypeptide biological potassium, 3 parts of gamma-polyglutamic acid, 6 parts of degradable super absorbent fiber, 0.3 part of photosynthetic inducer, 20 parts of mushroom leftovers and 15 parts of graft modified soybean protein.
Preferably, the drought-resistant matrix is prepared from the following raw materials in parts by weight:
30 parts of sandy soil, 12.5 parts of weathered coal, 1.35 parts of polysaccharide polypeptide biological potassium, 2 parts of gamma-polyglutamic acid, 4.5 parts of degradable super absorbent fiber, 0.2 part of photosynthetic inducer, 15 parts of mushroom leftovers and 12.5 parts of graft modified soybean protein.
Further, the mushroom leftovers are obtained by the following steps:
after the recycled mushroom leftovers are tidied and concentrated, 50% carbendazim wettable powder 600-time liquid is sprayed while stirring, and after uniform mixing, the mushroom leftovers are piled, stuffy and decomposed for 2-3 months.
The invention has the following beneficial effects:
the method can enhance the disease resistance and stress resistance of the alfalfa, and simultaneously enhance the water retention performance around the alfalfa rhizosphere, so that the alfalfa can still maintain the phenotype of a healthy plant under drought stress, and has higher biomass and water content of the overground part.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
S1, weighing the following components in parts by weight: 40 parts of sandy soil, 15 parts of weathered coal, 1.5 parts of polysaccharide polypeptide biological potassium, 3 parts of gamma-polyglutamic acid, 6 parts of degradable super absorbent fiber, 0.3 part of photosynthetic inducer, 20 parts of mushroom leftovers and 15 parts of graft modified soybean protein;
s2, crushing the weighed weathered coal and mushroom leftovers, and mixing and stirring the crushed weathered coal and mushroom leftovers with sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer and graft modified soybean protein uniformly to obtain the fertilizer.
Example 2
S1, weighing the following components in parts by weight: 40 parts of sandy soil, 15 parts of weathered coal, 1.5 parts of polysaccharide polypeptide biological potassium, 3 parts of gamma-polyglutamic acid, 6 parts of degradable super absorbent fiber, 0.3 part of photosynthetic inducer, 20 parts of mushroom leftovers and 15 parts of graft modified soybean protein;
s2, crushing the weighed weathered coal and mushroom leftovers, and mixing and stirring the crushed weathered coal and mushroom leftovers with sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer and graft modified soybean protein uniformly to obtain the fertilizer.
Example 3
S1, weighing the following components in parts by weight: 30 parts of sandy soil, 12.5 parts of weathered coal, 1.35 parts of polysaccharide polypeptide biological potassium, 2 parts of gamma-polyglutamic acid, 4.5 parts of degradable super absorbent fiber, 0.2 part of photosynthetic inducer, 15 parts of mushroom leftovers and 12.5 parts of graft modified soybean protein;
s2, crushing the weighed weathered coal and mushroom leftovers, and mixing and stirring the crushed weathered coal and mushroom leftovers with sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer and graft modified soybean protein uniformly to obtain the fertilizer.
Examples of the experiments
Experimental group 1: the substrate obtained in example 1;
experimental group 2: the substrate obtained in example 2;
experimental group 3: the substrate obtained in example 3;
control group 1: sandy soil;
control group 2: the polysaccharide polypeptide biological potassium is not contained, and the rest is the same as the embodiment 3;
control group 3: the same as example 3 except that gamma-polyglutamic acid was not contained;
control group 4: the same procedure as in example 3 was repeated except that the graft-modified soybean protein was not contained.
The experimental method comprises the following steps:
transferring alfalfa seedlings with radicles about 2cm long to plastic pots filled with different culture medium formulas for growth, transplanting 12 seedlings in each pot, and after one week of seedling revival, fixedly planting the number of seedlings in each pot into 8 seedlings. Watering twice every week, stopping watering after the alfalfa grows for 1 month, carrying out drought treatment, and collecting samples after the drought treatment for one month. The experiment was repeated three times and the results averaged.
1. Plant phenotype
The phenotype of the plants subjected to drought treatment for one month in different substrate formulas is observed, the leaves of the alfalfa in the control group 1 are severely wilted, typical drought stress symptoms appear, most (more than 60%) of the leaves in the control group 2 are severely wilted, a small part (less than 40%) of the leaves in the control group 3 are severely wilted, and a small part (less than 40%) of the leaves in the control group 4 are severely wilted. The alfalfa in the experimental group 1, the experimental group 2 and the experimental group 3 has stronger drought resistance, and leaves do not have wilting phenomenon, which shows that the substrate is favorable for the growth of the alfalfa under the drought condition.
2. Root system phenotype
The underground part of the plant was harvested and observed for root phenotype after one month of drought stress treatment in different medium formulations.
The alfalfa root system of the control group 1 is small, no adsorption matrix is arranged on the surface of the root system, the control group 2 is the third control group 3, and the fourth control group 4; the alfalfa in the experimental group 1, the experimental group 2 and the experimental group 3 has a large root system, and a large amount of wet substrates are adsorbed on the surface of the root system to form a root sheath, so that the water retention capacity around the rhizosphere is enhanced, and the drought resistance of the alfalfa is improved.
3. Comparison of Biomass
The phenotype of the plants grown drought for one month in the different substrate formulations was observed and the overground and underground biomass of the plants (referred to as the overground and underground dry weight) was measured by harvesting the plants. The biomass of the overground part and the underground part of the alfalfa in the control group 1 is the minimum, the biomass of the alfalfa in the control group 2 is the second, the control group 3 is the third, and the control group 4 is the fourth; the biomass of the aerial parts and the underground parts of the alfalfa in the experimental group 1, the experimental group 2 and the experimental group 3 is increased, and the biomass of the aerial parts is the largest in the example 3, which shows that the substrate provided by the invention is beneficial to increasing the yield of the alfalfa under the drought condition.
4. Water content of overground part
Harvesting alfalfa growing in different substrate formulations for one month under drought, weighing the fresh weight of the overground part, deactivating enzymes in an oven at 105 ℃ for half an hour, drying the plant material at 70 ℃ for at least 2 days, and measuring the dry weight of the overground part. The water content of the above-ground part was calculated from the difference between the fresh weight and the dry weight.
The water content of the above-ground parts of the alfalfa in the control group was small, the second control group 2, the third control group 3 and the fourth control group 4; the water content of the upper part of the alfalfa field of the experimental group 1, the experimental group 2 and the experimental group 3 is obviously increased, which shows that the substrate is beneficial to increasing the drought resistance of the alfalfa.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (4)
1. A drought-resistant matrix for cultivating alfalfa is characterized by being prepared from the following raw materials: sandy soil, weathered coal, polysaccharide polypeptide biological potassium, gamma-polyglutamic acid, degradable super absorbent fibers, photosynthetic inducer, mushroom leftovers and graft modified soybean protein;
the drought-resistant matrix for cultivating the alfalfa is prepared from the following raw materials in parts by weight:
20-40 parts of sandy soil, 10-15 parts of weathered coal, 1.2-1.5 parts of polysaccharide polypeptide biological potassium, 1-3 parts of gamma-polyglutamic acid, 3-6 parts of degradable super absorbent fibers, 0.1-0.3 part of photosynthetic inducer, 10-20 parts of mushroom leftovers and 10-15 parts of graft modified soybean protein;
the drought-resistant substrate for cultivating alfalfa is characterized in that mushroom leftovers are obtained through the following steps:
after the recycled mushroom leftovers are tidied and concentrated, 50% carbendazim wettable powder 600-time liquid is sprayed while stirring, and after uniform mixing, the mushroom leftovers are piled, stuffy and decomposed for 2-3 months.
2. The drought-resistant substrate for cultivating alfalfa as claimed in claim 1, wherein the drought-resistant substrate is prepared from the following raw materials in parts by weight:
20 parts of sandy soil, 10 parts of weathered coal, 1.2 parts of polysaccharide polypeptide biological potassium, 1 part of gamma-polyglutamic acid, 3 parts of degradable super absorbent fiber, 0.1 part of photosynthetic inducer, 10 parts of mushroom leftovers and 10 parts of graft modified soybean protein.
3. The drought-resistant substrate for cultivating alfalfa as claimed in claim 1, wherein the drought-resistant substrate is prepared from the following raw materials in parts by weight:
40 parts of sandy soil, 15 parts of weathered coal, 1.5 parts of polysaccharide polypeptide biological potassium, 3 parts of gamma-polyglutamic acid, 6 parts of degradable super absorbent fiber, 0.3 part of photosynthetic inducer, 20 parts of mushroom leftovers and 15 parts of graft modified soybean protein.
4. The drought-resistant substrate for cultivating alfalfa as claimed in claim 1, wherein the drought-resistant substrate is prepared from the following raw materials in parts by weight:
30 parts of sandy soil, 12.5 parts of weathered coal, 1.35 parts of polysaccharide polypeptide biological potassium, 2 parts of gamma-polyglutamic acid, 4.5 parts of degradable super absorbent fiber, 0.2 part of photosynthetic inducer, 15 parts of mushroom leftovers and 12.5 parts of graft modified soybean protein.
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| CN113396682A (en) * | 2021-07-28 | 2021-09-17 | 山西农业大学高寒区作物研究所(山西省农业科学院高寒区作物研究所) | Fertilizing method for remarkably improving yield of alfalfa |
| CN113443944A (en) * | 2021-08-02 | 2021-09-28 | 山西农业大学高寒区作物研究所(山西省农业科学院高寒区作物研究所) | Special organic compound fertilizer for alfalfa |
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| CN102515934B (en) * | 2011-11-07 | 2014-04-09 | 天津北洋百川生物技术有限公司 | Gamma-polyglutamic acid mixed fertilizer and desert treatment technology thereof |
| JP6514042B2 (en) * | 2015-06-04 | 2019-05-15 | 株式会社日健総本社 | Soil conditioner |
| CN106396904A (en) * | 2016-08-30 | 2017-02-15 | 山东胜伟园林科技有限公司 | Nutritional fertility enhancer for improving secondary saline land |
| CN107223483A (en) * | 2017-07-24 | 2017-10-03 | 福建省农业科学院生物技术研究所 | A kind of strawberry disease prevention cultivation matrix and preparation method thereof |
| CN107500947A (en) * | 2017-08-03 | 2017-12-22 | 河北省农林科学院石家庄果树研究所 | A kind of pears seedling culture matrix and preparation method thereof |
| CN109618867A (en) * | 2018-12-26 | 2019-04-16 | 中国科学院植物研究所 | It is a kind of cultivate clover drought-resistant substrate and its application |
| CN109729956A (en) * | 2018-12-26 | 2019-05-10 | 中国科学院植物研究所 | A kind of salt resistance matrix and its application for cultivating clover |
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