CN110638728A

CN110638728A - Skin care composition for removing acne and lightening acne marks and preparation method and application thereof

Info

Publication number: CN110638728A
Application number: CN201911113917.1A
Authority: CN
Inventors: 范玉菡; 龚德明; 温伟红; 莫霖霭
Original assignee: Guangzhou Biotechnology Co Ltd
Current assignee: Guangzhou Biotechnology Co Ltd
Priority date: 2019-11-14
Filing date: 2019-11-14
Publication date: 2020-01-03
Anticipated expiration: 2039-11-14
Also published as: CN110638728B

Abstract

The invention provides a skin care composition for removing acne and lightening mark, and a preparation method and application thereof, wherein the skin care composition comprises a witch hazel extract, an aloe leaf extract, a centella extract and a dragon blood leaf extract; the four natural plant components are mutually cooperated and matched, and the acne forming pathway is used for conditioning and repairing the damaged skin, reducing the secretion of skin grease, inhibiting bacteria, resisting inflammation, smoothing and stopping acne, and improving the immunity and the anti-allergy of the skin. The skin care composition for removing the acne and lightening the marks provided by the invention is simple in preparation method, can be applied to facial essence, cream, emulsion and facial masks, can reduce the grease secretion of the skin, repairs the damaged skin and erythema caused by acne, and has an obvious treatment effect.

Description

Skin care composition for removing acne and lightening acne marks and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a skin care composition for removing acne and removing acne marks, and a preparation method and application thereof.

Background

Acne, also known as pimple and comedo, is a chronic inflammatory dermatosis occurring in the pilosebaceous part, is one of the most common skin problems, and is easily developed in adolescents in adolescence. In recent years, more and more adults face acne due to environmental pollution, increased working pressure of urban life and the like. Acne affects the life, work and psychological confidence of the patient to various degrees, and therefore, the treatment of acne and the repair of skin are a non-negligible problem.

The clinical manifestations of acne are mainly characterized by various skin lesions such as acne, pimple, pustule, nodule and the like which are good at face or back, the etiology is complex, and the general cognition in the medical field at present is caused by the following five reasons: abnormal keratinization, microbial infection, inflammatory response, abnormal regulation of sebum secretion by androgens, and immune response. Clinically, acne is generally attributed to: a large amount of oil and fat substances in hair follicles and sebaceous glands and sweat, dust, germs, mites and the like which are not removed in time block sebaceous gland orifices on the surface of the skin, so that a large amount of pathogenic microorganisms (especially propionibacterium acnes) are induced to propagate, and a plurality of skin inflammations such as inflammatory papules, pustules, nodules, cysts and the like are formed. The existing common acne treatment methods comprise a pharmaceutical method and a physical method, wherein the pharmaceutical method can be divided into oral administration and external application, and common medicines comprise tretinoin, antibiotics, antiandrogens, glucocorticoids, traditional Chinese medicines and the like; physical methods include laser treatment and the like. Although the laser treatment has a certain treatment effect, the laser treatment has certain limitations and is not suitable for pregnant women, people with light sensitivity, people taking light sensitivity medicines, hypertension and diabetes patients and the like, so that the relatively universal acne treatment method is a medicine treatment method.

CN104997800A discloses an acne removing cream and a preparation method thereof, wherein the acne removing cream is prepared from a lincomycin hydrochloride/lidocaine hydrochloride mixture and a clindamycin/penicillin antibiotic mixture according to a ratio of 12:1, wherein the weight ratio of the lincomycin hydrochloride to the lidocaine hydrochloride is 8:7, and the weight ratio of the clindamycin to the penicillin is 0.75: 0.5. The acne removing cream effectively enhances the acne removing effect, has no pain and scar in the treatment process, does not cause the skin to have the conditions of allergy, inflammation and the like, has short treatment period, is not easy to relapse after healing, and has obvious curative effect on acne marks, acne, and other skin problems.

CN106821862A discloses an acne-removing composition and a preparation method thereof, wherein the acne-removing composition comprises the following components in parts by weight: 1-2 parts of salicylic acid, 1.2-2.8 parts of arginine, 0.1-2 parts of Bordeaux leaf extract, 0.2-0.5 part of silver bactericide, 1.2-10 parts of other acne-removing functional components and 0-90 parts of other base materials; wherein the other acne-removing effective components comprise cortex Phellodendri bark extract, milfoil extract, nicotinamide and tea tree essential oil. The acne-removing composition is prepared by neutralizing salicylic acid with arginine with a specific content, compounding with a Bordeaux leaf extract and assisting with a silver bactericide as an antiseptic bactericide, and has a good acne-removing effect.

CN105395717A discloses a traditional Chinese medicine acne removing cream for removing acnes and acne marks, which is prepared from the following raw materials: 5-10 parts of radix scutellariae, 5-10 parts of poria cocos, 5-10 parts of radix sophorae flavescentis, 5-10 parts of rhizoma coptidis, 5-10 parts of bunge corydalis herb, 1-5 parts of salvia officinalis and 1-5 parts of honeysuckle, and the acne cream is obtained by crushing and grinding the raw materials, soaking in hot water, decocting, concentrating and collecting the cream.

At present, products aiming at acne removal and skin repair are various in types, and effective active ingredients of the products comprise antibiotics, bactericides, traditional Chinese medicines and the like. The acne removing product containing the antibiotics can quickly eliminate red and swollen acne and relieve acne, but can affect the immune system of the skin, so that the skin is fragile and sensitive; the acne removing product containing the bactericide destroys the microecosystem of the skin while killing acne-causing bacteria, so that the skin loses a natural protective barrier; the traditional Chinese medicine products have relatively mild effects, but slow effect and long treatment period.

Therefore, the development of a mild and non-irritant skin care product with comprehensive anti-acne, anti-acne and anti-acne functions for fundamentally repairing and protecting the skin micro-ecosystem and the skin protection barrier is the research focus in the field.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide the skin care composition for removing the acne and lightening the acne mark and the preparation method and application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a skin care composition for removing acne marks, the skin care composition comprising a witch hazel extract, an aloe leaf extract, a centella asiatica extract, and a dragon's blood leaf extract.

The hamamelis extract is extracted from the bark, branches and leaves of hamamelis shrubs native to North America, and the hamamelis extract mainly comprises a plurality of tannins, flavonoids, gallic acid, catechin and volatile oil, and has remarkable effects of reducing skin oil secretion, resisting inflammation, resisting oxidation and resisting bacteria. The witch hazel extract can be obtained through market approaches and can also be obtained through extraction in the prior art; for example, the witch hazel extract can be extracted by combining acidic water extraction and ethanol extraction, so that the extraction rate and purity of effective component flavonoids (such as quercetin, astragalin and the like) are improved.

The main effective components of the folium Aloe extract are anthraquinone compounds (including aloin, aloe-emodin, aloe-chrysophanol and aloe saponin) and aloe polysaccharide, and have antibacterial, skin astringing, skin softening, sclerosing and moisturizing effects. The aloe leaf extract of the present invention is commercially available or may be extracted from aloe vera leaves by prior art methods, including, but not limited to, cold processing.

The centella asiatica extract mainly comprises triterpenes (asiaticoside and madecassoside), polyacetylene olefin compounds, flavonoid compounds and volatile oil, and has good effects of repairing skin injury, resisting bacteria and inflammation, treating scar and whitening skin. The centella asiatica extract according to the invention is available on the market, but also can be extracted from the leaves of centella asiatica by the prior art, for example using ethanol with the aid of ultrasound.

The dragon tree leaf extract is extracted from Harengana leaf which is native to Madagasca, and has main active ingredients including flavonoid, glucoside, tannin, saponin and alkaloid, and has antibacterial and antioxidant effects. The dragon tree leaf extract can be obtained through a market way, and can also be extracted from dragon tree leaves based on the prior art. Illustratively, the dracaena cochinchinensis leaf extract can be subjected to crude extraction by using water and/or ethanol, then refined and concentrated to obtain an extract with the flavonoid content of more than or equal to 10 percent by weight (based on the dry leaf extract).

The skin care composition for removing acnes and lightening spots provided by the invention is prepared by mutually and synergistically matching 4 natural plant components of a witch hazel extract, an aloe leaf extract, a centella extract and a dragon blood leaf extract, starts from a path formed by acne to condition and repair damaged skin, reduces skin grease secretion, inhibits bacteria and is anti-inflammatory, and relieves acne and pox. Different from products containing bactericides or antibiotics in the prior art, the skin care composition provided by the invention realizes the acne treatment effect through the mutual synergistic cooperation of active ingredients in plants, not only can not generate negative effects on a microecosystem and a protective barrier of skin, but also can radically repair the barrier function of damaged skin, improve the immunity of the skin and reduce the sensitivity.

In the skin care composition provided by the invention, all plant extracts can be obtained through market or can be obtained through extraction by a known method disclosed in the prior art, and the extracts obtained by different extraction methods do not cause the difference of the technical effects of the skin care composition provided by the invention.

Preferably, the skin care composition comprises the following components in percentage by mass:

in the present invention, the witch hazel extract may be contained in an amount of 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 1.9%, or the like, by mass.

The aloe leaf extract may be 0.13%, 0.15%, 0.18%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 4.9% by mass.

The centella asiatica extract may be present in an amount of 0.03%, 0.05%, 0.08%, 0.1%, 0.13%, 0.15%, 0.18%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 3.9%, or the like, by mass.

The mass percentage content of the dragon tree leaf extract can be 0.03%, 0.05%, 0.08%, 0.1%, 0.3%, 0.5%, 0.7%, 0.9%, 1%, 1.1%, 1.3%, 1.4%, 1.5%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8% or 2.9% and the like.

Preferably, the mass ratio of the dragon blood leaf extract to the asiatic centella extract is 1 (0.8-1.5), such as 1:0.85, 1:0.9, 1:0.95, 1:1, 1:1.05, 1:1.1, 1:1.15, 1:1.2, 1:1.25, 1:1.3, 1:1.35, 1:1.4 or 1: 1.45.

According to the preferable technical scheme, the mass ratio of the dragon blood leaf extract to the centella asiatica extract in the skin care composition is 1 (0.8-1.5), and the dragon blood leaf extract and the centella asiatica extract can be synergized to the maximum extent within the range of the mixture ratio, so that the technical effects of bacteriostasis (especially propionibacterium acnes), anti-inflammation and reduction of damaged skin sensitivity are achieved.

preferably, the skin care composition further comprises agave stem extract.

Preferably, the content of the agave stem extract in the skin care composition is 0.01-1% by mass, such as 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 0.95%.

Preferably, erythritol is also included in the skin care composition.

Preferably, erythritol is present in the skin care composition in an amount of 0.1 to 1% by mass, for example, 0.13%, 0.15%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 0.95%.

Preferably, sodium hyaluronate is also included in the skin care composition.

Preferably, the content of sodium hyaluronate in the skin care composition is 0.5-3% by mass, such as 0.6%, 0.7%, 0.9%, 1%, 1.1%, 1.3%, 1.4%, 1.5%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8% or 2.9%.

The skin care composition provided by the invention also comprises a agave stem extract, erythritol and sodium hyaluronate, wherein the chemical name of the erythritol is 1,2,3, 4-erythritol, and the hydroxyl group of the erythritol endows the erythritol with excellent water-locking and moisture-keeping properties; the sodium hyaluronate has special water retention function and can absorb 100 times of water by weight of the sodium hyaluronate; the agave stem extract has an inhibiting effect on hyaluronidase in the skin, can effectively inhibit the decomposition of sodium hyaluronate, and protects hyaluronic acid from exerting a long-acting moisturizing function in the skin. The erythritol, the sodium hyaluronate and the agave stem extract are cooperatively matched, so that the water storage and moisturizing capability of damaged skin is improved, and the barrier function of the skin is repaired.

in another aspect, the present invention provides a method for preparing the skin care composition for removing acne and lightening marks, the method comprising: mixing and dispersing the components to obtain the skin care composition for removing the acne and lightening the marks.

Preferably, the dispersion is carried out under stirring conditions.

Preferably, the temperature of the dispersion is 20 to 90 ℃, such as 22 ℃, 25 ℃, 28 ℃, 30 ℃, 33 ℃, 35 ℃, 37 ℃, 40 ℃, 43 ℃, 45 ℃, 48 ℃, 50 ℃, 55 ℃, 60 ℃, 65 ℃, 70 ℃, 75 ℃, 80 ℃, 85 ℃ or 89 ℃, and more preferably 20 to 50 ℃.

Preferably, the dispersing time is 10-40 min, such as 12min, 15min, 18min, 20min, 22min, 25min, 27min, 30min, 32min, 35min, 37min or 39 min.

The skin care composition is prepared by mixing and uniformly dispersing all the components, and post-treatment steps such as filtration and the like can also be included in the preparation process. The skin care composition is characterized in that the plant components of the witch hazel extract, the aloe leaf extract, the centella extract and the dracaena cochinchinensis leaf extract and the agave stem extract in the skin care composition are mixed and dispersed at a lower temperature (20-50 ℃), so that the inactivation of active ingredients in the plant extracts caused by high temperature is avoided; the mixing and dispersing temperature of the components such as sodium hyaluronate, erythritol and the like can be increased to 70-90 ℃ to ensure full dissolution and dispersion of the components.

Illustratively, the skin care composition is prepared by: sequentially adding sodium hyaluronate, erythritol and water into a vacuum emulsifying pot according to the formula amount, homogenizing and dispersing uniformly, heating to 70-90 ℃, and keeping the temperature and stirring for 5-10 min; and cooling to 20-50 ℃, adding a witch hazel extract, an aloe leaf extract, a centella extract, a dragon blood leaf extract and a agave stem extract, stirring for 10-30 min, and uniformly mixing to obtain the skin care composition.

In another aspect, the present invention provides a skin care product comprising the skin care composition for removing acne marks as described above.

Preferably, the skin care product comprises any one of facial essence, mask, cream or lotion.

Preferably, the skin care product is a facial essence, and the content of the skin care composition in the facial essence is 0.1-50% by mass, such as 0.12%, 0.15%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 16%, 18%, 20%, 23%, 25%, 27%, 30%, 33%, 35%, 38%, 40%, 42%, 45%, 47%, or 49%.

Preferably, the facial essence further comprises at least one of a solvent, a humectant, a thickener, a pH adjuster, a preservative, a solubilizer or a fragrance.

Preferably, the solvent is water.

Preferably, the humectant is selected from any one or a combination of at least two of propylene glycol, butylene glycol, glycerin or hexylene glycol.

Preferably, the facial essence contains the moisturizing agent in an amount of 1-20% by mass, for example, 1.2%, 1.5%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9%, 10%, 12%, 14%, 16%, 18%, 19%, or the like.

Preferably, the thickener is a carbomer.

Preferably, the content of the thickener in the facial essence is 0.1-1% by mass, such as 0.12%, 0.15%, 0.18%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, or 0.9%.

Preferably, the pH adjusting agent is triethanolamine.

Preferably, the content of the pH regulator in the facial essence is 0.1-1% by mass, such as 0.12%, 0.15%, 0.18%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, or 0.9%.

Preferably, the preservative is selected from any one or a combination of at least two of methylparaben, phenoxyethanol or ethylhexyl glycerin.

Preferably, the facial essence contains 0.2-1% of preservative by mass, such as 0.22%, 0.25%, 0.28%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, or 0.9%.

Preferably, the solubilizer is PEG-40 hydrogenated castor oil.

Preferably, the mass percentage of the solubilizer in the facial essence is 0.03-0.1%, such as 0.035%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09% or 0.095%.

Preferably, the fragrance is a perfume.

Preferably, the facial essence contains 0.01-0.3% of the flavoring agent, such as 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, 0.13%, 0.15%, 0.18%, 0.2%, 0.23%, 0.25%, 0.27% or 0.29% by weight.

Preferably, the skin care product is facial essence which comprises the following components in percentage by mass: the skin care composition for removing acne and lightening acne marks comprises 0.1-50% of a humectant, 0.1-1% of a thickening agent, 0.1-1% of a pH regulator, 0.2-1% of a preservative, 0.03-0.1% of a solubilizer, 0.01-0.3% of an aromatic and the balance of water.

The facial essence is prepared by mixing and uniformly dispersing all the components, and the preparation process can also comprise post-treatment steps such as filtration and the like. Illustratively, the preparation method of the facial essence comprises the following steps: sequentially adding the thickening agent, the humectant and water into a vacuum emulsifying pot according to the formula amount, homogenizing and dispersing uniformly, heating to 70-90 ℃, and keeping the temperature and stirring for 5-10 min; and cooling to 20-50 ℃, adding the acne and acne removing skin care composition, a pH regulator, a preservative, a solubilizer and an aromatic, stirring for 10-30 min, uniformly mixing, cooling to below 40 ℃, filtering by using filter cloth, and discharging to obtain the facial essence.

Compared with the prior art, the invention has the following beneficial effects:

the skin care composition for removing acnes and lightening spots comprises a witch hazel extract, an aloe leaf extract, a centella extract and a dragon blood leaf extract, and the four natural plant components are cooperated with each other, so that a path formed by acne is used for conditioning and repairing damaged skin, secretion of skin oil is reduced, bacteria and inflammation are inhibited, acne is removed, and immunity and allergy resistance of the skin are improved. The skin care composition for removing acnes and lightening spots provided by the invention can obviously inhibit and kill propionibacterium acnes and staphylococcus aureus, promote the expression of anti-inflammatory cytokine interleukin 10, obviously reduce the expression level of proinflammatory cytokines and inhibit histamine release by synergistically compounding the components in a specific ratio. The skin care composition for removing the acne and lightening the marks has good functions of removing the acne and repairing the acne damaged skin, can reduce the grease secretion of the skin by 30 percent after being used for 4 weeks, reduces the erythema number by 50 percent, has no adverse case and has very obvious treatment effect.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Examples 1 to 6

The skin care composition for removing the acne and lightening the mark comprises the following specific components in parts by weight as shown in Table 1.

TABLE 1

The preparation method comprises the following steps:

sequentially adding sodium hyaluronate, erythritol and water into a vacuum emulsifying pot according to the formula amount, homogenizing and dispersing uniformly, heating to 85 ℃, and keeping the temperature and stirring for 10 min; cooling to 45 ℃, adding a witch hazel extract, an aloe leaf extract, a centella extract, a dragon blood leaf extract and a agave stem extract, stirring for 15min, uniformly mixing, cooling to 38 ℃, filtering by using a 200-mesh filter cloth, and discharging to obtain the skin care composition for removing acne and light marks.

Comparative example 1

This comparative example differs from example 1 in that it does not contain witch hazel extract.

Comparative example 2

This comparative example differs from example 1 in that aloe leaf extract is not contained.

Comparative example 3

This comparative example differs from example 1 in that it does not contain centella asiatica extract.

Comparative example 4

This comparative example differs from example 1 in that it does not contain the dragon's blood leaves extract.

Comparative example 5

This comparative example differs from example 1 in that the centella asiatica extract is replaced by an equal weight part of the dragon's blood leaves extract.

Comparative example 6

This comparative example differs from example 1 in that the dragon's blood leaves extract is replaced by an equal part by weight of centella asiatica extract.

Comparative example 7

This comparative example differs from example 1 in that the witch hazel extract was replaced with equal parts by weight of aloe leaf extract.

Comparative example 8

This comparative example differs from example 1 in that the aloe leaf extract was replaced with an equal weight part of witch hazel extract.

In vitro cell assay testing:

firstly, bacteriostatic experiment:

(1) preparing the drug sensitive tablet: respectively soaking sterile circular filter paper with the diameter of 6mm into the skin care composition for removing acne and lightening marks provided by the examples 1-6 and the comparative examples 1-8, taking out after 30min, and drying in the shade to obtain a drug sensitive tablet;

(2) selecting a propionibacterium acnes colony by using a sterilization inoculating loop, placing the propionibacterium acnes colony in sterile normal saline to prepare a bacterial suspension, and correcting the turbidity of the bacterial suspension to be 0.5 McLee unit; the bacteria solution is uniformly coated on an anaerobic brain-heart infusion flat plate by using a sterile cotton swab, after being dried for 5min, the drug sensitive tablets of examples 1 to 6 and comparative examples 1 to 8 are respectively stuck on the bacteria solution, and then the bacteria solution is put into an anaerobic jar (10% CO)₂、10％H₂、80％N₂) Culturing at 37 ℃ for 48h, taking out, measuring the diameter of the drug sensitive tablet bacteriostatic circle by a cross method, and subtracting 6mm from the measured diameter to obtain the diameter of the bacteriostatic circle;

(3) adding 20mL of nutrient medium into a sterile culture dish, drying, condensing water for solidification, and adhering staphylococcus aureus liquid (about 10%) with a sterile cotton swab⁵CFU/mL) is uniformly coated on the surface of a flat plate, after no liquid flows on the flat plate, the drug sensitive tablets of examples 1-6 and comparative examples 1-8 are respectively stuck, the culture is carried out at the constant temperature of 37 ℃, and after 24 hours, the diameter of a bacteriostatic circle is measured by a cross method; the zone diameter data is shown in table 2.

TABLE 2

	Propionibacterium acnes/mm	Staphylococcus aureus/mm
			Example 1	18.7	17.0
Example 2	16.3	15.7
			Example 3	19.5	17.4
Example 4	18.4	16.8
			Example 5	15.0	15.4
Example 6	15.3	15.0
			Comparative example 1	10.3	9.4
Comparative example 2	10.7	9.2
			Comparative example 3	11.1	10.0
Comparative example 4	12.9	12.1
			Comparative example 5	8.7	8.0
Comparative example 6	9.5	8.6
			Comparative example 7	10.5	9.3
Comparative example 8	11.1	8.9

As can be seen from the data in table 2, the skin care composition for removing acne and lightening marks provided in embodiments 1 to 6 of the present invention has an obvious growth inhibition and killing effect on propionibacterium acnes and staphylococcus aureus, forms an inhibition zone with a diameter greater than 15mm, can effectively inhibit bacteria and achieve an acne treatment effect by conditioning the micro-ecological environment of damaged skin. The antibacterial effect of the skin care composition in the embodiments 1 to 4 is better than that of the embodiments 5 to 6, which shows that the mass ratio of the dragon tree leaf extract to the centella asiatica extract is more than the preferable 1 (0.8 to 1.5) in the invention, so that the antibacterial effect of the skin care composition is reduced.

The skin care composition for removing acne and lightening marks has the antibacterial and antibacterial effects which are realized by mutually matching four plant components including a witch hazel extract, an aloe leaf extract, a centella asiatica extract and a dragon blood leaf extract, and the diameter of an antibacterial ring is reduced and the antibacterial and antibacterial effects are weakened due to the absence of any one plant component (comparative examples 1-4). In addition, the four plant extracts have a synergistic effect, and particularly, the antibacterial effect achieved by the synergistic combination of the centella asiatica extract and the dragon blood leaf extract is obviously superior to that of an excessive single component (comparative examples 5-6), and the antibacterial effect achieved by the synergistic combination of the witch hazel extract and the aloe leaf extract is obviously superior to that of an excessive aloe leaf extract or an excessive witch hazel extract (comparative examples 7-8).

II, anti-inflammatory experiment:

(1) cell collection: mixing the blood sample with the blood cell separation solution in equal proportion, centrifuging at 20 deg.C for 40min, collecting human peripheral blood mononuclear cell layer, washing cells with buffer solution, and suspending the cells with culture medium RPMI-1640; CD14+ monocytes in human peripheral blood mononuclear cells were then purified with CD14+ microspheres (MiniMACS system); the differentiation of cells into dendritic cells is stimulated by the cytokine IL-4 and the growth hormone GM-CSF at 37 ℃ with 5% CO₂Culturing for 6 days, collecting differentiated dendritic cells and inoculating the dendritic cells into a pore plate;

(2) the skin care compositions for removing acne and lightening marks provided in example 1 and comparative examples 5 to 8 were added to the well plate at a concentration of 2 μ g/mL (calculated as the content of plant extract in the skin care composition), and the wells to which the medium of the same volume was added were set as blank control at 37 ℃ with 5% CO₂Co-culturing for 48 hours; the cell culture supernatant was collected. The enzyme-linked immunosorbent assay (ELISA) is used for detecting the content of the interleukins IL-10 and IL-12 in the supernatant, the content of the anti-inflammatory cytokine IL-10 in the blank control group is defined as 0, the content of the proinflammatory cytokine IL-12 is defined as 100%, and the test results are shown in Table 3.

TABLE 3

	Interleukin IL-10/%)	Interleukin IL-12/%)
			Blank control group	0	100
Example 1	161	6
			Comparative example 5	95	71
Comparative example 6	83	76
			Comparative example 7	125	54
Comparative example 8	107	69

According to the data in table 3, the skin care composition for removing acne and lightening marks provided in example 1 of the present invention can significantly increase the level of anti-inflammatory cytokine interleukin IL-10, and inhibit the expression level of proinflammatory cytokine interleukin IL-12, which proves that the skin care composition of the present invention has a good anti-inflammatory effect.

Comparing the data of example 1 with comparative examples 5-8, it can be seen that the anti-inflammatory effect of the skin care composition is obtained based on the synergistic cooperation of four natural plant components, namely, the witch hazel extract, the aloe leaf extract, the centella asiatica extract and the dragon blood leaf extract, and the components have synergistic effect and non-substitutability, the synergistic combination effect of the centella asiatica extract and the dragon blood leaf extract is obviously superior to the anti-inflammatory effect of excessive single component (comparative examples 5-6), and the synergistic combination effect of the witch hazel extract and the aloe leaf extract is obviously superior to the anti-inflammatory effect of excessive aloe leaf extract or excessive witch hazel extract (comparative examples 7-8). In addition, the anti-inflammatory effects of comparative examples 7-8 were better than comparative examples 5-6, demonstrating that centella asiatica extract and dragon's leaves extract contributed more to the anti-inflammatory efficacy of the skin care composition.

Thirdly, antiallergic experiment:

histamine is often stored in mast cells of human tissues, and when a human body is stimulated by external stimuli to trigger an antigen-antibody reaction, the permeability of cell membranes of the mast cells is changed to release histamine, and the histamine and a histamine receptor generate pathophysiological effects, so that the histamine release concentration is one of indexes for evaluating allergic reactions.

KU812 cells have high similarity to mast cells, and an antiallergic experiment was performed using KU812 cells as model cells: KU812 cells were cultured at 2.0X 10⁵The density of each well is inoculated in 12-well plate, 37 ℃, 5% CO₂Cells adhere to the wall after 24h of culture; the skin care compositions for removing acne and lightening marks provided in example 1 and comparative examples 5 to 8 were added at a concentration of 5. mu.g/mL per well (calculated as the content of plant extract in the skin care composition), at 37 ℃ and 5% CO₂Co-culturing for 48 h; discarding the culture medium and adding 1mL of Tyrode solution to balance for 10 min; 50 μ L C48/80(10 μ g/mL) was added to each well, a blank control group was set and 50 μ L of Tyrode solution was added, the reaction was terminated after 30min, the supernatant and cell pellet were separated, the histamine content was measured by histamine fluorescence, and the calculated histamine inhibition rate is shown in Table 4.

TABLE 4

	Histamine inhibition rate/%)
		Example 1	41.2
Comparative example 5	21.1
		Comparative example 6	32.8
Comparative example 7	22.5
		Comparative example 8	27.4

According to the data in table 4, the inhibition rate of the skin care composition for removing acne and pale spots provided in embodiment 1 of the invention on histamine is more than 40%, which is obviously higher than the inhibition rate of the skin care composition in comparative examples 5 to 8 on histamine, and the synergistic combination of the four active ingredients of the witch hazel extract, the aloe leaf extract, the centella asiatica extract and the dragon's blood leaves extract in the invention is proved to be capable of effectively inhibiting histamine release and realizing the anti-allergic effect.

Application example 1

The facial essence comprises the following components in percentage by mass: example 1 provides a skin care composition that removes acne marks 40%, butylene glycol 2%, propylene glycol 5%, glycerin 10%, triethanolamine 0.3%, methylparaben 0.3%, phenoxyethanol 0.1%, ethylhexylglycerin 0.1%, PEG-40 hydrogenated castor oil 0.08%, carbomer 3%, perfume 0.2%, and balance water.

The preparation method comprises the following steps: sequentially adding carbomer, butanediol, propylene glycol, glycerol and water into a vacuum emulsifying pot according to the formula amount, homogenizing and dispersing uniformly, heating to 85 ℃, and keeping the temperature and stirring for 10 min; cooling to 40 deg.C, adding the skin care composition, triethanolamine, methyl hydroxybenzoate, phenoxyethanol, ethylhexyl glycerol, PEG-40 hydrogenated castor oil and essence, stirring for 20min, mixing, cooling to below 35 deg.C, filtering with 200 mesh filter cloth, and discharging to obtain the facial essence.

And (3) performance testing:

30 acne patients (half of men and women) aged 18-45 years are used as test volunteers and are randomly divided into 15 treatment groups and 15 control groups. After the face of the tested volunteer is cleaned, a VISIA facial image analyzer is used for carrying out facial shooting, the facial skin damage condition and the range of an acne area are analyzed, the skin oil content is detected at the cheek, the forehead and the chin, and the average value obtained by 3 times of testing of each part is used as an initial value. The treatment group applied the facial essence provided in application example 1 to the acne affected part 2 times a day (1 time each morning and evening), and the control group applied a placebo containing no skin care composition of the present invention to the acne affected part 2 times a day (1 time each morning and evening), for 4 weeks as a treatment course. The information obtained by the VISIA facial image analyzer after the end of a treatment period was compared with the information before treatment, and the skin lesion improvement, erythema number and oil fat rate of the test volunteers were analyzed, and the statistical data are shown in table 5.

TABLE 5

Compared with the results of the VISIA test before and after treatment, no adverse reaction cases occur during the treatment, the total skin feel is good and fresh, the skin damage condition and the color of the affected part of the tested volunteer are obviously improved, the erythema condition left by acne is obviously reduced, the proportion of the reduction of the erythema number can reach more than 50 percent, the skin oil content is obviously reduced, the reduction proportion reaches 30 percent, and the curative effect evaluation is effective.

The applicant states that the present invention is illustrated by the above examples to the acne and acne mark removing skin care composition of the present invention and the preparation method and application thereof, but the present invention is not limited to the above examples, i.e. it does not mean that the present invention must be implemented by the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims

1. A skin care composition for removing acne and lightening marks, which is characterized by comprising a witch hazel extract, an aloe leaf extract, a centella asiatica extract and a dragon blood tree leaf extract.

2. The skin care composition according to claim 1, wherein the skin care composition comprises the following components in percentage by mass:

3. the skin care composition as claimed in claim 1 or 2, wherein the mass ratio of the dragon blood leaf extract to the centella asiatica extract is 1 (0.8-1.5);

4. the skin care composition according to any one of claims 1 to 3, further comprising a Agave stem extract;

preferably, the mass percentage content of the agave stem extract in the skin care composition is 0.01-1%;

preferably, erythritol is also included in the skin care composition;

preferably, the mass percentage content of the erythritol in the skin care composition is 0.1-1%;

preferably, sodium hyaluronate is also included in the skin care composition;

preferably, the mass percentage content of the sodium hyaluronate in the skin care composition is 0.5-3%;

5. a method for preparing the acne-removing and acne-whitening skin care composition according to any one of claims 1 to 4, wherein the method comprises the following steps: mixing and dispersing the components to obtain the skin care composition for removing the acne and lightening the marks.

6. The production method according to claim 5, wherein the dispersion is performed under stirring conditions;

preferably, the temperature of the dispersion is 20-90 ℃, and further preferably 20-50 ℃;

preferably, the dispersing time is 10-40 min.

7. A skin care product comprising the skin care composition for removing acne marks according to any one of claims 1 to 4.

8. The skin care product of claim 8, wherein the skin care product comprises any one of a facial essence, a mask, a cream or a lotion.

9. The skin care product according to claim 7 or 8, wherein the skin care product is facial essence, and the skin care composition for removing acne and lightening marks in the facial essence is 0.1-50% by mass;

preferably, the facial essence further comprises at least one of a solvent, a humectant, a thickener, a pH regulator, a preservative, a solubilizer or a fragrance;

preferably, the solvent is water;

preferably, the humectant is selected from any one or a combination of at least two of propylene glycol, butylene glycol, glycerin or hexylene glycol;

preferably, the facial essence contains the moisturizing agent in an amount of 1-20% by mass;

preferably, the thickener is a carbomer;

preferably, the mass percentage of the thickening agent in the facial essence is 0.1-1%;

preferably, the pH adjusting agent is triethanolamine;

preferably, the mass percentage of the pH regulator in the facial essence is 0.1-1%;

preferably, the preservative is selected from any one or a combination of at least two of methyl hydroxybenzoate, phenoxyethanol or ethylhexyl glycerol;

preferably, the facial essence contains 0.2-1% of preservative by mass;

preferably, the solubilizer is PEG-40 hydrogenated castor oil;

preferably, the mass percentage of the solubilizer in the facial essence is 0.03-0.1%;

preferably, the fragrance is a perfume;

preferably, the mass percentage of the aromatic in the facial essence is 0.01-0.3%.

10. The skin care product according to any one of claims 7 to 9, wherein the skin care product is a facial essence, and the facial essence comprises the following components in percentage by mass: the skin care composition for removing acne and lightening acne marks comprises 0.1-50% of a humectant, 0.1-1% of a thickening agent, 0.1-1% of a pH regulator, 0.2-1% of a preservative, 0.03-0.1% of a solubilizer, 0.01-0.3% of an aromatic and the balance of water.