CN110637918A - Preparation method for producing digestion-free protein by using soybean protein isolate as raw material - Google Patents
Preparation method for producing digestion-free protein by using soybean protein isolate as raw material Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention discloses a method for producing digestion-free protein by using isolated soy protein as a raw material. Indigestible protein refers to small molecule proteins with a molecular weight below 500 daltons. The components of the polypeptide are composite amino acid with average molecular weight of 125 Dalton, small molecular dipeptide and tripeptide with average molecular weight of 180 to 500 Dalton. The protein segment does not need to be digested by human body, and can directly enter the body through the small intestine of the human body to be absorbed and utilized. The quality of products in domestic peptide industry is good and bad, which means that the content of dipeptide and tripeptide is determined, because the peptides with the length of more than 500 daltons are not directly absorbed and utilized by human bodies, and the products of the process are basically not digested for the second time after being eaten by human bodies. The membrane separation technology can be adopted on the production line, so that the high-purity digestion-free protein product can be produced, and the digestion-free protein product can also be produced without adopting a purification process.
Description
Technical Field
The invention relates to a food of a protein enzyme deep processing technology, in particular to a digestion-free protein which can be directly absorbed by the small intestine of a human body with the molecular weight below 500Da and is converted from macromolecular soybean protein through the shearing action of enzyme. The components of the polypeptide are composite amino acid with average molecular weight of 125 Dalton, small molecular dipeptide and tripeptide with average molecular weight of 180 to 500 Dalton. The protein segment does not need to be digested by human body, and can directly enter the body through the small intestine of the human body to be absorbed and utilized. The quality of products in domestic peptide industry is good and bad, which means that the content of dipeptide and tripeptide is determined, because the peptides with the length of more than 500 daltons are not directly absorbed and utilized by human bodies, and the products of the process are basically not digested for the second time after being eaten by human bodies and cannot be utilized.
Background
According to the latest theories of life: it is considered that one of the main causes of aging in the elderly is insufficient recovery and utilization of endogenous cellular proteins, and long-term shortage of a nitrogen source in the body is a main cause of aging. The theory considers that the life cycle of human intestinal cells is only 2 days at most, the life cycle of gastric surface cells is three days, the old cells are shed as new cells grow out, the cells are digested and utilized by the human body like food, and normal people have 8-inch two, namely 248.8 g of intestinal cell protein shed every day. Half a kilogram of optimal human body cell protein with the biological potency of 100 does not consider the desquamated cell protein of the stomach, the cell protein of the intestine is not entwined by gastric acid denaturation treatment molecules, is very tight and is not easy to digest, and the normal young people can completely recycle the protein after the long-time digestion at the lower end of the small intestine. The old people can not completely recycle endogenous cell protein due to the decline of all functions of the aged organism, especially the reduction of the amount and activity of digestive enzymes, so that the long-term nitrogen source of the organism is unbalanced. It is clear what the body's long-term protein deficiency is as a result, why old people lose muscle so badly that the body cells use their own muscle proteins for survival. The body is in a state of vicious circle due to lack of nitrogen source and protein, the old has no way, the digestive function of the body is reduced, the digestive function is large, and the protein which is eaten by people is not digested, and the protein is not absorbed and utilized. In addition, the protein is not digested and fermented in the intestinal tract to generate a plurality of toxins such as skatole and the like, and the burden of the kidney is increased. It is known that the reason is that smelly fart is caused when people eat more meat, and the digestion-free protein product is the only method for solving the problem of protein deficiency of the old.
Indigestible proteins are proteins with a molecular weight below 500 daltons. Wherein the compound amino acid has an average molecular weight of 125Da and the small molecular dipeptide and tripeptide have molecular weights of 180-500 Da. Scientists have discovered during the study of genes that the human body has a substance that determines all functional activities of the human body's life, called peptides. He speaks: peptide is a revolutionary product that will lead the human nutritional revolution in the 21 st century. The human has been undergoing three health revolution since history, the first smallpox appeared, the solution was the vaccinium nikoense, the second penicillin was used to treat the disease caused by bacteria and virus, the third was the use of small molecule active peptide to treat all diseases including all major diseases, which was the problem of cell failure. Medical science states that only one disease of humans is cellular failure. Cell failure is due to 2 causes, 1, the cells are not nourished. (not getting the needed things) 2, cytotoxic overload. (get something not needed) if someone poisons your food and supplies, what happens.
Scientists have found a substance capable of prolonging life and have experimented with in mice, which was successful for the first time, the magical substance was a mixture of 3 amino acids leucine, isoleucine and valine, known by the scientific name "branched chain amino acids". In the experimental process, researchers add the 3 amino acids into water drunk by the tested mice, and as a result, the lives of the 3 amino acids are prolonged by 12%, and a series of positive biological reactions occur in the bodies of the mice while the lives of the 3 amino acids are prolonged, for example, cells can absorb more energy, free radicals are reduced, and the like, the free radicals are harmful compounds generated in oxidation reactions of the bodies, have strong oxidizing property, can damage tissues and cells of the bodies, and further cause chronic diseases and aging reactions. Through these in vivo changes, the mice appear to be fully active and have improved muscle coordination. Researchers have described that "branched chain amino acids" are an important and effective nutritional supplement that helps the organism strengthen muscles or gain more energy naturally without any side effects. The researchers in this project, professor Enzunesi of Milan university, Italy, indicate that amino acid nutritional supplements are invented on this basis, and then the amino acid nutritional supplements can benefit human beings in the future and have great help for the health of the old or patients.
The technology is a digestion-free protein product produced by using isolated soy protein as a raw material through an enzyme technology, wherein the compound amino acid contains branched chain amino acid, the branched chain amino acid consists of leucine, valine and isoleucine, and the isolated soy protein contains the following components in percentage by weight: 6.7266%, 4.0198% and 3.6899% in total, 14.4363%. The branched chain amino acid, so the development of the product has great significance to health.
Objects of the invention
The method aims to solve the problem that the old people have insufficient protein due to the fact that cell protein in the body is not recycled sufficiently due to aging of the body, the old product of protein powder is replaced by the biotechnology, a new product of the biotechnology with higher value is provided, the protein balance of the old people is achieved, and the most important for human health is nutrient balance and the reduction of body toxins. The protein food can supplement protein products which are not digested for the old, and has great health effect on the old.
The preparation process comprises the following steps:
weighing soybean protein isolate as a raw material;
secondly, mixing the raw materials with water of which the ratio is 1 to 7-8 times that of the raw materials;
carrying out ultra-high temperature sterilization, passivation of physiological harmful substances and proper protein denaturation treatment on the material liquid subjected to size mixing, wherein the temperature is at least 135 ℃, and the heat preservation is carried out for more than 50 seconds;
fourthly, cooling the third step to reduce the temperature of the feed liquid to 54-56 ℃;
fifthly, adding the compound protease with the weight of 0.3-0.5% of the weight of the raw materials into the mixture to carry out enzymolysis for 48-72 hours, wherein the pH value does not need to be adjusted in the enzymolysis process, but the mixture needs to be stirred slowly;
sixthly, the feed liquid after the enzymolysis is purified by membrane separation, and the purified feed liquid is subjected to enzyme deactivation treatment at 90 ℃ for 30 seconds to produce a high-purity digestion-free protein product;
seventhly, the feed liquid after enzymolysis is not subjected to membrane separation and purification, and the feed liquid is subjected to enzyme deactivation treatment at 90 ℃ for 30 seconds to produce a digestion-free protein isolate product;
eighthly, blending the material liquid after enzymolysis according to the standard of the liquid product, and carrying out aseptic filling to obtain the liquid product;
ninthly, concentrating the feed liquid to obtain solid matter over 40%, and spray drying to obtain powder product.
The invention has the following advantages and positive effects:
1. the enzyme engineering of biotechnology is utilized to upgrade and replace the traditional protein powder product into a digestion-free protein product. The upgraded and updated product has the function of being absorbed without digestion, and has great significance for the aged with digestive function decline and the people in a sick state, and the liberation military fighters who can eliminate fatigue and recover physical strength as soon as possible and the digestion-free protein product.
2. Branched chain amino acid products are provided and studies have shown that: the branched chain amino acid can make the cell take more energy, reduce free radical and resist oxidation. Free radicals are harmful compounds generated in oxidation reactions of the body, have strong oxidizing properties, and can damage tissues and cells of the body, thereby causing chronic diseases and aging reactions. The nutritional supplement containing the body-strengthening branch and the amino acid has great help for the health of the elderly or patients.
3. Provides a small molecule peptide product which is really free from digestion in the market. The small molecular peptide is a component of human immune cells, and can proliferate the human immune cells and activate the human immune cells after entering a body. American shake video: the whole process of killing cancer cells by immune cells in a human body is also realized by the principle of the existing immunotherapy that the immune cells are injected into the human body after being proliferated in vitro, so that the immune cells complete the task of killing the cancer cells, and the small molecular peptide has the effect of preventing and treating cancers. The peptide obtained by human body has only two ways, namely, the small molecular dipeptide and tripeptide with the edible molecular weight of 180-500 Da. Secondly, the injection is injected into the body. Insulin, for example, is the 51 peptide, which saves countless lives of diabetics worldwide.
The specific implementation mode is as follows:
example 1:
1. weighing 100kg of soybean protein isolate;
2. mixing soybean protein isolate material with 7-8 times of water;
3. sterilizing the mixed material liquid at ultrahigh temperature, passivating physiological harmful substances and performing protein denaturation treatment, and keeping the temperature at least 135 ℃ for more than 50 seconds;
4. cooling the material liquid to 54-56 deg.c;
5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, wherein 0.3 kg of compound protease is added in the example, the enzymolysis time is 70 hours, and the pH value is not required to be adjusted in the enzymolysis process, but the slow stirring treatment is required;
6. carrying out membrane separation on the enzymolysis feed liquid to remove indigestible protein larger than 500Da, and carrying out enzyme deactivation treatment on the feed liquid for 30 seconds at 90 ℃ after purification to obtain a high-purity indigestible protein product;
7. the feed liquid after membrane separation and enzyme deactivation is processed by blending, aseptic filling and liquid product according to the standard of the liquid product;
8. the feed liquid after membrane separation and enzyme deactivation is concentrated by a double-effect evaporator to a solid content of more than 40 percent, and then spray drying is carried out to obtain a powdery product.
Example 2:
1. weighing 200kg of soybean protein isolate;
2. mixing soybean protein isolate material with 7-8 times of water;
3. sterilizing the mixed material liquid at ultrahigh temperature, passivating physiological harmful substances and performing protein denaturation treatment, and keeping the temperature at least 135 ℃ for more than 50 seconds;
4. cooling the material liquid to 54-56 deg.c;
5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, wherein in test 2, 0.6 kg of compound protease is added, the enzymolysis time is 70 hours, and the pH value does not need to be adjusted in the enzymolysis process, but the slow stirring treatment is needed;
6. the enzymolysis feed liquid is not subjected to membrane separation and purification, and is subjected to enzyme deactivation treatment at 90 ℃ for 30 seconds. Obtaining an active digestion-free protein product;
7. blending the feed liquid according to the standard of liquid products, and performing aseptic filling to obtain two liquid products;
8. concentrating the above feed liquid by double effect evaporator to solid content of above 40%, and spray drying to obtain two kinds of powder products.
Claims (4)
1. A preparation method for producing digestion-free protein by using isolated soy protein as a raw material; the digestion-free protein is an upgraded and updated product of soybean protein isolate, and the components of the digestion-free protein are mainly a product which is composed of composite amino acid with the average molecular weight of 125 daltons, small molecular dipeptide with the average molecular weight of 180-500 daltons and tripeptide; the soybean protein isolate is used as a raw material to cut off macromolecular protein through enzyme engineering, so that the protein product which can be directly absorbed and utilized by a human body without digestion is achieved.
2. The process adopts a membrane separation and purification method to separate out protein larger than 500Da to produce a high-purity digestion-free protein product, and can also produce a digestion-free soybean protein isolate product without purification after enzymolysis.
3. The feed liquid after the digestion-free protein enzymolysis can be prepared according to the liquid product standard to produce liquid products, and the liquid products are concentrated, sprayed and dried to produce powdery products;
the preparation process comprises the following steps:
weighing soybean protein isolate raw materials;
secondly, mixing water with the weight of 1 to 7 to 8 times of that of the raw materials;
carrying out ultra-high temperature sterilization, passivation of physiological harmful substances and proper protein denaturation treatment on the mixed feed liquid, and keeping the temperature at least 135 ℃ for more than 50 seconds;
fourthly, cooling the third step to reduce the temperature of the feed liquid to 54-56 ℃;
fifthly, adding the compound protease with the weight of 0.3-0.5% of the weight of the raw materials into the mixture to carry out enzymolysis for 48-72 hours, wherein the pH value does not need to be adjusted in the enzymolysis process, but the mixture needs to be stirred slowly;
sixthly, the feed liquid after the enzymolysis is purified by membrane separation, and the purified feed liquid is subjected to enzyme deactivation treatment at 90 ℃ for 30 seconds to produce a high-purity digestion-free protein product;
seventhly, the feed liquid after enzymolysis is not subjected to membrane separation and purification, and the feed liquid is subjected to enzyme deactivation treatment at 90 ℃ for 30 seconds to produce a digestion-free protein isolate product;
eighthly, blending the liquid state of the sixteenth step and the sixteenth step according to the standard of the liquid state product, and carrying out aseptic filling to obtain the liquid state product;
ninthly, concentrating the feed liquid to obtain solid matter over 40%, and spray drying to obtain powder product.
4. The method for preparing the digestion-free protein by using the isolated protein as the raw material according to the claims 1, 2 and 3, which is characterized in that: the method takes soybean protein isolate as a raw material, and obtains high-purity digestion-free protein liquid and powder products and digestion-free protein isolate liquid and powder products through size mixing, enzymolysis, membrane separation or non-membrane separation.
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