CN112825958A - Method for preparing digestion-free double protein by using live beads and soybean protein as raw materials - Google Patents

Abstract

The invention discloses a method for preparing digestion-free double protein by taking live beads and soybean protein as raw materials, which belongs to biological food. Digestion-free protein refers to protein with a molecular weight of 500Da (Dalton), wherein the average molecular weight is 120Da and the molecular weight is 180-500 Da. The live beads are digestion-free double-protein products obtained by taking fertilized eggs as raw materials and performing enzymolysis on the fertilized eggs by taking the early live embryoid bodies incubated for 8 to 14 days and soybean protein as the raw materials.

Description

Method for preparing digestion-free double protein by using live beads and soybean protein as raw materials

Technical Field

The invention relates to a preparation method of biological food, in particular to a method for preparing digestion-free double protein by using live beads and soybean protein as raw materials.

Background

According to the latest theories of life and physiology: when the human body is about 25 years old, the growth period is over 30 years old, and the maturity period is over, all the functions of the human body can begin to decline, and the functions decline greatly after 60 years old. The new theory suggests that the main reason of aging of the old people is that endogenous cellular protein can not be completely recycled, and the long-term shortage of body protein is the main reason of premature aging of the old people. The physiology states that: the life cycle of human intestinal cells is 43 hours at most, the life cycle of cells on the surface of the stomach is three days, old cells are exfoliated as new cells grow out, the cells are digested and utilized by the human body like food, and normal people have 8-inch and two-inch exfoliated cells, namely 248.8 g of intestinal cell proteins are exfoliated every day. Half jin of human body cell protein with the biological value of 100 is the best quality, the falling cell protein of the stomach is not considered, the cell protein of the small intestine is not denatured by gastric acid, the molecule winding is very tight and is not easy to digest, and the normal young people can completely recycle the protein after the long-time digestion at the lower end of the small intestine.

The human protein supply consists of three parts:

the first part supplies: is cell protein dropped from intestinal tract, the dropped cell is digested and utilized like food, adult has at least 8 engbi and 31.1 g per engbi every day, namely 248.8 g of dropped cell protein, the cell protein is human protein, is the best quality protein with 100 biological potency, and the biological potency of any other kind of protein is lower than the best quality protein.

A second part supplying: is dead cells in tissues, about 198-283 g of dead cells are repeatedly used by autophagy of human bodies every day, and autophagy means that the dead cells eat themselves. The existence of enzymes in living human cells that digest proteins, carbohydrates and fats can digest apoptotic cells for reuse by the cells is called autophagy. Looking up "one day hungry secret" autophagy science on the web, doctor university swedish japan scientist in 2016 thus won the nobel prize.

The third part supplies: is protein in food, and is supplemented by 1.0 g to 1.5 g per kilogram of body weight per day. The protein in food is digested in stomach, the protease secreted by human stomach is inactive and activated by gastric acid to digest the protein in food, and the aging of human body is the decrease of secretion in body which directly affects the digestion of protein in body.

The old people can not completely recycle endogenous cell protein due to the aging of each function of the organism, especially the reduction of the secretion amount and the reduction of the activity of digestive enzyme, so that the long-term nitrogen source imbalance of the organism is caused. It is clear what the body's long-term protein deficiency is as a result, why old people lose muscle so badly that the body cells use their own muscle proteins for survival. The protein-deficient organism is in a state of vicious circle, the old has no way, the digestive function of the body is reduced, and the protein in the body is not digested, namely the protein is absorbed and utilized. And the protein is not digested and fermented in the intestinal tract to generate a plurality of toxins such as skatole, and the toxins are absorbed by the large intestine to hurt the body. It is known that this is why eating too much meat gives off bad fart.

The digestion-free protein product is the only method for solving the problem of protein deficiency of the old. Protein nutrition plays an important role in the aging process, and nutrient imbalance not only accelerates organ aging, but also causes diseases. Another manifestation of aging in the elderly is: the metabolism is slowed down, the digestion function is reduced, in particular, various hormones in the body are reduced, the hormones are important substances which are generated by the human body and used for regulating the normal activities of the body, the hormone secretion in the body is out of balance and must be supplemented from the aging angle through an external source, the embryo of the live bead is rich in rich bioactive substances, and various biological hormones have important nutritional values for the old.

The indigestible protein is a protein with a molecular weight below 500 Da. Wherein the compound amino acid has an average molecular weight of 120Da and the small molecular dipeptide and tripeptide have molecular weights of 180-500 Da. Scientists have discovered during the study of genes that the human body has a substance that determines all functional activities of the human body's life, called peptides. He speaks: peptide is a revolutionary product that will lead the human nutritional revolution in the 21 st century. The human has been undergoing three health revolution since history, the first smallpox appeared, the solution was the vaccinium nikoense, the second penicillin was used to treat the disease caused by bacteria and virus, the third was the use of small molecule active peptide to treat all diseases including all major diseases, which was the problem of cell failure. Medical science states that only one disease of humans is cellular failure. Cell failure is due to two causes, 1, the cells are not nourished. (not getting the needed things) 2, cytotoxic overload. (unwanted material is obtained).

Reported in the uk daily telegraph: since ancient times, the life of the human is not long-lived, the dream does not seem to be out of reach, scientists find a substance capable of prolonging the life, and experiments are carried out on mice, the first time the scientific substance is successful, and the magical substance is a mixture of 3 amino acids, namely leucine, isoleucine and valine, and is known as 'branched chain amino acid'. In the experimental process, researchers add the 3 amino acids into water drunk by the tested mice, and as a result, the lives of the 3 amino acids are prolonged by 12%, and a series of positive biological reactions occur in the bodies of the mice while the lives of the 3 amino acids are prolonged, for example, cells can absorb more energy, free radicals are reduced, and the like, the free radicals are harmful compounds generated in oxidation reactions of the bodies, have strong oxidizing property, can damage tissues and cells of the bodies, and further cause chronic diseases and aging reactions. Through these in vivo changes, the mice appear to be fully active and have improved muscle coordination.

Researchers have described that "branched chain amino acids" are an important and effective nutritional supplement that helps the organism strengthen muscles or gain more energy naturally without any side effects. The researchers in this project, professor Enzunesi of Milan university, Italy, indicate that amino acid nutritional supplements are invented on this basis, and then the amino acid nutritional supplements can benefit human beings in the future and have great help for the health of the old or patients. The technology takes soybean protein and coffee bean protein as raw materials, and the branched chain amino acid (BACC) of the digestion-free protein product produced by the enzyme technology accounts for 17-18.33 percent of the protein, so the development of the product has great significance to health.

The invention aims to solve the problem that the old people have insufficient protein in vivo due to the insufficient recovery and utilization of cell protein in vivo caused by the aging of the body, realize the in vitro digestion by using a biological enzyme engineering technology, provide a protein product which can be directly absorbed through small intestines without digestion, solve the problem of protein unbalance of the old people, and ensure that the most important substance for human health is protein and no life exists without protein. In addition, the living embryo is rich in nutrient active substances and various biological hormones, and the important substances are reduced after the human body is aged, so that the vital substances can be supplemented very importantly.

Disclosure of Invention

The invention aims to provide a protein food which is digested in vitro for the old, and solves the problem of pain point of protein nutrient imbalance caused by incomplete digestion of protein due to aging of the old. Another manifestation of aging in elderly persons while providing protein products containing branched chain amino acids is: the metabolism is slowed down, the digestion function is reduced, in particular, various hormones in the body are reduced, the hormones are important substances which are generated by the human body and used for regulating the normal activities of the body, the hormone secretion in the body is out of balance and must be supplemented from the aging angle through an external source, the embryo of the live bead is rich in rich bioactive substances, and various biological hormones have important nutritional values for the old. Therefore, the development of the digestion-free double-protein product is significant to the health of the old.

The preparation process comprises the following steps:

firstly, mixing soybean protein isolate powder raw material with water of which the ratio is 9 to 10 times that of the soybean protein isolate powder raw material;

preparing live bead feed liquid, incubating in a fertilized egg sterilization incubator, controlling the temperature to be between 37.8 and 42 ℃, and within 8 to 14 days of incubation time, completely selecting live embryos, and adding 4 to 5 times of sterile water for crushing and refining;

thirdly, mixing the first step and the second step, heating for sterilization and protein denaturation treatment, wherein the temperature is required to be at least 130 ℃ for 60 seconds;

fourthly, the temperature of the heated feed liquid is adjusted to 54 to 56 ℃, 0.3 to 0.5 percent of compound protease is added, the mixture is stirred at a low speed, the temperature is controlled to be between 54 and 56 ℃ all the time, the deep enzymolysis time is between 36 and 72 hours, and the pH value does not need to be adjusted in the whole enzymolysis process;

fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished to obtain digestion-free double-protein liquid feed liquid;

sterilizing and inactivating enzyme, wherein the temperature of the feed liquid is up to 90-95 ℃ through a sterilizer, and keeping the temperature for 15-25 seconds to achieve the purpose of sterilizing and inactivating enzyme;

seventhly, concentrating the materials by the double-effect evaporator to more than 40 percent, and performing spray drying to obtain a coffee digestive protein-free powder product;

and eighthly, mixing the sterilized and enzyme-deactivated liquid material according to the standard of the liquid product, and performing aseptic filling to obtain the liquid product.

2. The method for preparing the digestion-free double-protein product by using the live beads and the soybean protein as the raw materials according to claim 1 is characterized in that the digestion-free double-protein product with the molecular weight of less than 500 daltons is produced by using the live beads and the soybean protein as the raw materials and performing compound enzyme degradation and digestion.

The invention has the following advantages and positive effects:

1. in order to solve the problem that the old people have insufficient protein due to the insufficient recovery and utilization of in vivo cell protein caused by the aging of the body, the enzyme engineering of the biotechnology is utilized to upgrade the traditional soybean protein product into a coffee indigestion-free protein product. The product after upgrading has the function of being absorbed without digestion, and can be used for the old with hypofunction of digestion, the people in a sick state and the people who can eliminate fatigue and recover physical performance as soon as possible. In addition, another manifestation of aging in the elderly is: the metabolism is slowed down, the digestion function is reduced, in particular, various hormones in the body are reduced, the hormones are important substances which are generated by the human body and used for regulating the normal activities of the body, the hormone secretion in the body is out of balance and must be supplemented from the aging angle through an external source, the embryo of the live bead is rich in rich bioactive substances, and various biological hormones have important nutritional values for the old. Therefore, the development of the digestion-free double-protein product is significant to the health of the old.

2. Branched chain amino acid products are provided and studies have shown that: the branched chain amino acid can make the cell take more energy, reduce free radical and resist oxidation. Free radicals are harmful compounds generated in oxidation reactions of the body, have strong oxidizing properties, and can damage tissues and cells of the body, thereby causing chronic diseases and aging reactions. The nutritional supplement containing the body-strengthening branch and the amino acid has great help for the health of the elderly or patients.

3. Provides a small molecule peptide product which is really free from digestion in the market. The small molecular peptide is a component of human immune cells, and can proliferate the human immune cells and activate the human immune cells after entering a body. American shake video: the whole process of killing cancer cells by immune cells in a human body, and the principle of the existing immunotherapy is that the immune cells are injected into the human body after being proliferated in vitro, so that the immune cells complete the task of killing the cancer cells, so that: proliferating human immune cells is the most effective method for preventing and treating cancer.

The peptide obtained by human body has only two ways, namely, the small molecular dipeptide and tripeptide with the edible molecular weight of 180 Da-500 Da. Secondly, the peptide product is injected into the body through injection. Insulin, for example, is the 51 peptide, which saves countless lives of diabetics worldwide.

Detailed Description

Example 1:

1. soybean protein isolate weighed 100kg, live beads 15kg (preparation of live beads: fertilized egg after sterilization, hatching 8 to 14 days of live embryo in 37.8 to 40.5 degrees);

2. mixing soybean protein isolate material with 9-10 times of water, adding crushed live bead liquid, and requiring the crushing fineness to be more than 100 meshes;

3. carrying out ultra-high temperature sterilization, passivation of physiological harmful substances and protein denaturation treatment on the mixed feed liquid, and keeping the temperature at least 130 ℃ for more than 60 seconds;

4. cooling the material liquid to 54-56 deg.c;

5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, adding 0.4 kg of compound protease in the example, wherein the enzymolysis time is 70-75 hours, the pH value is not required to be adjusted in the enzymolysis process, and the temperature is required to be between 54-56 ℃ and needs to be stirred slowly for treatment;

6. carrying out centrifugal separation treatment on the enzymolysis feed liquid to remove insoluble substances, and then carrying out enzyme deactivation treatment on the feed liquid at 90 ℃ for 30 seconds to obtain digestion-free double-protein feed liquid;

7. blending the feed liquid after enzyme deactivation according to the standard of the liquid product, and aseptically filling to obtain the liquid product;

8. concentrating the feed liquid after enzyme deactivation by a double-effect evaporator to a solid content of more than 40%, and performing spray drying to obtain a powdery product.

Example 2:

1. weighing 200kg of soybean high-temperature soybean meal and 17kg of live beads (preparation of live beads: incubation of live embryos for 8 to 14 days at 37.8 to 40.5 ℃ after sterilization of fertilized eggs);

2. mixing soybean meal raw material with 9-10 times of water, adding crushed live bead liquid, and crushing the mixture to a fineness of more than 100 meshes;

3. carrying out ultra-high temperature sterilization, passivation of physiological harmful substances and protein denaturation treatment on the mixed feed liquid, and keeping the temperature at least 130 ℃ for more than 60 seconds;

4. cooling the material liquid to 54-56 deg.c;

5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, in example 2, adding 0.8 kg of compound protease, wherein the enzymolysis time is 70-75 hours, the pH value does not need to be adjusted in the enzymolysis process, but the slow stirring treatment is needed, and the centrifugal separation is needed after the enzymolysis;

6. finely filtering and purifying the separated feed liquid, and performing enzyme deactivation treatment at 90 ℃ for 30 seconds to obtain digestion-free double-protein feed liquid;

7. blending the feed liquid according to the standard of liquid products, and performing aseptic filling to obtain two liquid products;

8. concentrating the feed liquid by a double-effect evaporator to a solid content of more than 40%, and performing spray drying to obtain a powdery product.

Claims (4)

1. A method for preparing digestion-free double protein by using live beads and soybean protein as raw materials is characterized in that: the live beads (which refer to live embryo bodies hatched from fertilized eggs for 8 to 14 days) and the soybean protein are prepared from the following raw materials in parts by weight: 9-10 parts of isolated soy protein and 1-5 parts of live beads (based on fertilized eggs hatched for 8-14 days in early living embryos); or 9-10 parts of high-low temperature soybean meal and 0.8-3 parts of live beads (based on fertilized eggs hatched for 8-14 days in early living embryos);

the preparation process comprises the following steps:

firstly, mixing soybean protein isolate powder raw material with water of which the ratio is 9 to 10 times that of the soybean protein isolate powder raw material;

preparing live bead feed liquid, incubating in an incubator after sterilizing fertilized eggs, controlling the temperature to be between 37.8 and 42 ℃, completely selecting live embryos within 8 to 14 days, adding 4 to 5 times of sterile water for crushing and refining, separating protein from soybeans, and crushing high-temperature and low-temperature soybean meal together;

thirdly, mixing the first step and the second step, heating for sterilization and protein denaturation treatment, and keeping the temperature at least 130 ℃ for more than 60 seconds;

fourthly, the temperature of the feed liquid is adjusted to 54 ℃ to 56 ℃, 0.3 percent to 0.5 percent of compound protease is added, the mixture is stirred at a low speed, the temperature is controlled to be between 54 ℃ and 56 ℃ all the time, the deep enzymolysis time is between 36 hours and 72 hours, and the pH value does not need to be adjusted in the whole enzymolysis process;

fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished to obtain digestion-free double-protein feed liquid;

sterilizing and inactivating enzyme, wherein the temperature of the feed liquid is up to 90-95 ℃ through a sterilizer, and keeping the temperature for 15-25 seconds to achieve the purpose of sterilizing and inactivating enzyme;

seventhly, concentrating the solid matter of the material to be more than 40 percent by a double-effect evaporator, and carrying out spray drying to obtain a digestion-free double-protein powder product;

and eighthly, mixing the sterilized and enzyme-deactivated liquid material according to the standard of the liquid product, and performing aseptic filling to obtain the digestion-free double-protein liquid product.

2. The method for preparing indigestible double protein using live beads and soybean protein as raw materials according to claim 1, wherein the method comprises using early live beads and soybean protein as raw materials, and producing an indigestible double protein product with a molecular weight of less than 500 daltons by complex enzyme digestion.

3. The method for preparing digestion-free double protein by using live beads and soybean protein as raw materials according to claim 1, wherein the method comprises the following steps: the early live beads refer to living embryos hatched by fertilized eggs from 8 days to 14 days and soybean protein isolate powder or edible high-low temperature meal which are used as raw materials.

4. The method for preparing digestion-free double protein by using live beads and soybean protein as raw materials according to claim 1, wherein the method comprises the following steps:

firstly, mixing soybean protein isolate powder raw material with water of which the ratio is 9 to 10 times that of the soybean protein isolate powder raw material;

preparing live bead feed liquid, incubating in an incubator after sterilizing fertilized eggs, controlling the temperature to be between 37.8 and 42 ℃, completely selecting live embryos within 8 to 14 days, adding 4 to 5 times of sterile water to crush and refine the live embryos and the soybean protein isolate, and crushing the live embryos and the soybean meal together;

thirdly, mixing the first step and the second step, heating for sterilization and protein denaturation treatment, and keeping the temperature at least 130 ℃ for more than 60 seconds;

fourthly, the temperature of the feed liquid is adjusted to 54 ℃ to 56 ℃, 0.3 percent to 0.5 percent of compound protease is added, the mixture is stirred at a low speed, the temperature is controlled to be between 54 ℃ and 56 ℃ all the time, the deep enzymolysis time is between 36 hours and 72 hours, and the pH value does not need to be adjusted in the whole enzymolysis process;

fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished to obtain digestion-free double-protein feed liquid;

sterilizing and inactivating enzyme, wherein the temperature of the feed liquid is up to 90-95 ℃ through a sterilizer, and keeping the temperature for 15-25 seconds to achieve the purpose of sterilizing and inactivating enzyme;

seventhly, concentrating the materials by a double-effect evaporator until the solid content is more than 40 percent, and performing spray drying to obtain a digestion-free double-protein powder product;

and eighthly, mixing the sterilized and enzyme-deactivated liquid material according to the standard of the liquid product, and performing aseptic filling to obtain the digestion-free double-protein liquid product.