CN111387336A - Preparation method of high branched chain amino acid digestion-free corn protein product - Google Patents
Preparation method of high branched chain amino acid digestion-free corn protein product Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of a high branched chain amino acid digestion-free corn protein product. The invention takes corn protein powder as raw material to carry out biological enzymolysis treatment, and obtains the digestion-free corn protein product with the branched chain amino acid content of about 22.24 percent. The indigestible protein product is small molecular protein with molecular weight below 500 Da. The components of the polypeptide are composite amino acid with the average molecular weight of 120Da and small molecular dipeptide and tripeptide with the average molecular weight of 180-500 Da. The protein segment does not need to be digested by human body, and can directly enter the body through the small intestine of the human body to be absorbed and utilized. Provides a protein product which can be absorbed without digestion for the society, and the aging is caused by insufficient protein because the degradation of digestive enzymes and the reduction of activity of various functions of the human body are reduced and the protein can not be completely digested and absorbed after the human body is old.
Description
Technical Field
The invention relates to a food of a protein enzyme deep processing technology, in particular to a digestion-free protein which can be directly absorbed by the small intestine of a human body with the molecular weight below 500Da and is converted from macromolecular zein through the shearing action of enzyme. The components of the polypeptide are composite amino acid with average molecular weight of 125 Dalton, small molecular dipeptide and tripeptide with average molecular weight of 180 to 500 Dalton. The protein segment does not need to be digested by human body, and can directly enter the body through the small intestine of the human body to be absorbed and utilized. The quality of products in domestic peptide industry is good and bad, which means that the content of dipeptide and tripeptide is determined, because the peptides with the content of more than 500 daltons are not directly absorbed and utilized by human bodies, and the products of the process are basically not digested for the second time after being eaten by human bodies, because the products have a layer of protective film, the products cannot be digested for the second time and utilized.
Background
According to the latest theories of life: the main reason for aging of the old is considered to be insufficient recycling of endogenous cell protein, and the main reason for aging is the lack of body nitrogen source caused by long-term shortage. The theory considers that the life cycle of human intestinal cells is 43 hours, the life cycle of cells on the surface of the stomach is three days, the old cells are exfoliated as new cells grow out, the cells are digested and recycled by the human body like food, and normal people have 8-inch intestinal cell proteins, namely 248.8 g of intestinal cell proteins exfoliated every day. Half a kilogram of optimal human body cell protein with the biological potency of 100 does not consider the desquamated cell protein of the stomach, the cell protein of the intestine is not entwined by gastric acid denaturation treatment molecules, is very tight and is not easy to digest, and the normal young people can completely recycle the protein after the long-time digestion at the lower end of the small intestine. The source of human protein is satisfied by the combination of endogenous cell-free protein and the digestion of food protein into amino acid, the ratio of cell protein to food protein is about 6: 1, the biological potency of cell protein is 100 points, and the protein in food is 50 to 92 points, depending on the ratio of 8 essential amino acids that the human body can not synthesize. In addition: the corn protein also contains glutathione, which can generate glutathione oxidase under the participation of selenium, and the component has the functions of anti-aging, anti-aging and delaying aging.
The old people can not completely recycle endogenous cell protein due to the aging of each function of the organism, especially the reduction of the secretion amount and the reduction of the activity of digestive enzyme, so that the long-term nitrogen source imbalance of the organism is caused. It is clear what the body's long-term protein deficiency is as a result, why old people lose muscle as severely, and that body tissues draw on their own muscle proteins for survival. The protein-deficient organism is in a state of vicious circle, the old has no way, the digestive function of the body is reduced, and the protein in the body is not digested, namely the protein is absorbed and utilized. And the protein is not digested and fermented in the intestinal tract to generate a plurality of toxins such as skatole, and the toxins are absorbed by the large intestine to hurt the body. It is known that the reason is that smelly fart is caused when people eat more meat, and the digestion-free protein product is the only method for solving the problem of protein deficiency of the old.
The indigestible protein is a protein with a molecular weight below 500 Da. Wherein the compound amino acid has an average molecular weight of 120Da and the small molecular dipeptide and tripeptide have molecular weights of 180-500 Da. Scientists have discovered during the study of genes that the human body has a substance that determines all functional activities of the human body's life, called peptides. Also: peptide is a revolutionary product that will lead the human nutritional revolution in the 21 st century. The human has been undergoing three health revolution since history, the first smallpox appeared, the solution was the vaccinium nikoense, the second penicillin was used to treat the disease caused by bacteria and virus, the third was the use of small molecule active peptide to treat all diseases including all major diseases, which was the problem of cell failure. Medical science states that only one disease of humans is cellular failure. Cell failure is due to 2 causes, 1, the cells are not nourished. (not getting the needed things) 2, cytotoxic overload. (unwanted material is obtained).
Scientists have found a substance capable of prolonging life and have experimented with in mice, which was successful for the first time, the magical substance was a mixture of 3 amino acids leucine, isoleucine and valine, known by the scientific name "branched chain amino acids". In the experimental process, researchers add the 3 amino acids into water drunk by the tested mice, and as a result, the lives of the 3 amino acids are prolonged by 12%, and a series of positive biological reactions occur in the bodies of the mice while the lives of the 3 amino acids are prolonged, for example, cells can absorb more energy, free radicals are reduced, and the like, the free radicals are harmful compounds generated in oxidation reactions of the bodies, have strong oxidizing property, can damage tissues and cells of the bodies, and further cause chronic diseases and aging reactions. Through these in vivo changes, the mice appear to be fully active and have improved muscle coordination. Researchers have described that "branched chain amino acids" are an important and effective nutritional supplement that helps the organism strengthen muscles or gain more energy naturally without any side effects. The researchers in this project, professor Enzunesi of Milan university, Italy, indicate that amino acid nutritional supplements are invented on this basis, and then the amino acid nutritional supplements can benefit human beings in the future and have great help for the health of the old or patients.
The technology takes corn protein as raw material, and produces the digestion-free corn protein product of high branched chain amino acid through enzyme technology, and the branched chain amino acid (BACC) accounts for 22.24 percent of the protein. Therefore, the development of the product has great significance to health.
Technology has developed quite rapidly, but how the components of food are transformed into energy and substances required by human life remains a mysterious palace that modern science needs to explore. The human body is like a chemical plant, in which 45 essential nutrients are used to construct cells and to enable the cells to perform normal activities. The alimentary canal of the food from the mouth to the anus is physically and chemically modified into easily soluble substances, and the nutrients in the substances are absorbed by the tissues of partial alimentary canal and then transported to 100 trillion cells needing nutrients. Finally, the nutrients are absorbed for the construction of human tissue and the production of energy.
Disclosure of Invention
The invention aims to solve the problem that the old people have insufficient protein due to insufficient recovery and utilization of in-vivo cell protein caused by aging of the body, and the invention produces a digestion-free protein product of high branched chain amino acid by using biotechnology, provides a new product of more valuable biotechnology, solves the problem of protein unbalance of the old people, and has the most important significance for human health in balancing nutrients and reducing body toxins, so that the protein product of high branched chain amino acid which is not digested is produced for the old people, and has great effect on the health of the old people.
The preparation process comprises the following steps:
①, weighing the corn protein powder as raw materials;
②, mixing ① raw materials with water in a ratio of 1: 8-10 times;
③, carrying out ultra-high temperature sterilization and appropriate protein denaturation treatment on ② mixed slurry, wherein the temperature is at least 135 ℃ and is required to be kept for more than 50 seconds;
④, cooling ③ to reduce the temperature of the feed liquid to 54-56 ℃;
⑤, adding ④ into compound protease accounting for 0.3-0.5 percent of the weight of the raw materials for enzymolysis for 48-72 hours, wherein the pH value does not need to be adjusted in the enzymolysis process, but the raw materials need to be stirred slowly;
⑥, purifying the feed liquid after ⑤ enzymolysis by membrane separation, and carrying out enzyme deactivation treatment or enzyme deactivation treatment on the purified feed liquid at 90 ℃ for 30 seconds to produce a digestion-free corn protein product with high purity, enzyme activity, non-activity and high branched chain amino acid;
⑦, carrying out enzymolysis on ⑤, and then carrying out enzyme deactivation treatment or enzyme deactivation treatment on the feed liquid at 90 ℃ for 30 seconds without membrane separation and purification to produce a digestion-free corn protein product with enzyme activity and inactive high-branched-chain amino acid;
⑧ concentrating ⑥ and ⑦, blending according to the standard of liquid product, and aseptically packaging to obtain liquid product;
⑨ concentrating ⑥ and ⑦ and the liquid to obtain solid matter over 40%, and spray drying to obtain powder.
The invention has the following advantages and positive effects:
1. the corn protein is used as a raw material by utilizing a biological enzyme engineering technology to produce a digestion-free corn protein product with high branched chain amino acid enzyme activity and non-activity, and a branched chain amino acid mouse experiment has prolonged the service life of a mouse by 12 percent and provides a new biological food capable of prolonging the service life of the society. In addition, the digestion-free protein product is the only method for solving the problem of social pain points that the protein cannot be digested and utilized due to the degradation of digestive function caused by aging. According to 2018, 2.49 hundred million people over 60 years old in the country account for 17.9 percent of the total population in the country. Therefore, the product has great significance for the old with hypofunction of digestion and the people in the sick state, and the liberation military fighters and students and the digestion-free protein product which can eliminate fatigue and recover physical ability as soon as possible.
2. Branched chain amino acid products are provided and studies have shown that: the branched chain amino acid can make the cell take more energy, reduce free radical and resist oxidation. Free radicals are harmful compounds generated in oxidation reactions of the body, have strong oxidizing properties, and can damage tissues and cells of the body, thereby causing chronic diseases and aging reactions. The nutritional supplement containing the body-strengthening branch and the amino acid has great help for the health of the elderly or patients.
3. Provides a small molecule peptide product which is really free from digestion in the market. The small molecular peptide is a component of human immune cells, and can proliferate the human immune cells and activate the human immune cells after entering a body. American shake video: the whole process of killing cancer cells by immune cells in a human body is also realized by the principle of the existing immunotherapy that the immune cells are injected into the human body after being proliferated in vitro, so that the immune cells complete the task of killing the cancer cells, and the small molecular peptide has the effect of preventing and treating cancers. The peptide obtained by human body has only two ways, namely, the small molecular dipeptide and tripeptide with the edible molecular weight of 180-500 Da. Secondly, the injection is injected into the body. Insulin, for example, is the 51 peptide, which saves countless lives of diabetics worldwide.
Detailed description of the preferred embodiments
Example 1:
1. weighing 100kg of corn protein powder;
2. mixing corn protein raw material with 8-10 times of water, and adding 1000kg of water in the experiment;
3. carrying out ultra-high temperature sterilization and protein denaturation treatment on the mixed feed liquid at the temperature of at least 135 ℃ for more than 50 seconds;
4. cooling the material liquid to 54-56 deg.c;
5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, wherein 0.3 kg of compound protease is added in the example, the enzymolysis time is 68 hours, and the pH value is not required to be adjusted in the enzymolysis process, but the slow stirring treatment is required;
6. separating the enzymolysis feed liquid by a membrane to remove indigestible protein larger than 500Da, purifying, and performing enzyme deactivation treatment or enzyme deactivation treatment on the feed liquid for 30 seconds at 90 ℃ to obtain high-purity and non-high-purity, enzyme activity and non-active high-branched-chain amino acid indigestible corn protein products;
7. concentrating the four feed liquids, blending according to the standard of liquid products, and aseptically filling to obtain four liquid products;
8. the material liquid of the treatment process 6 is concentrated by a double-effect evaporator to a solid content of more than 40 percent, and then is spray-dried to obtain four powdery products.
Example 2:
1. weighing 200kg of corn protein powder;
2. mixing soybean protein isolate raw material with 8-10 times of water, adding 2000kg of water in the experiment;
3. sterilizing the mixed material liquid at ultrahigh temperature, passivating physiological harmful substances and performing protein denaturation treatment, and keeping the temperature at least 135 ℃ for more than 50 seconds;
4. cooling the material liquid to 54-56 deg.c;
5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, wherein 0.6 kg of compound protease is added in the example, the enzymolysis time is 68 hours, and the pH value is not required to be adjusted in the enzymolysis process, but the slow stirring treatment is required;
6. separating the enzymolysis feed liquid by a membrane to remove indigestible protein larger than 500Da, purifying, and performing enzyme deactivation treatment or enzyme deactivation treatment on the feed liquid for 30 seconds at 90 ℃ to obtain high-purity and non-high-purity, enzyme activity and non-active high-branched-chain amino acid indigestible corn protein products;
7. concentrating the four feed liquids, blending according to the standard of liquid products, and aseptically filling to obtain four liquid products;
8. the material liquid of the treatment process 6 is concentrated by a double-effect evaporator to a solid content of more than 40 percent, and then is spray-dried to obtain four powdery products.
Claims (5)
1. The invention relates to a preparation method of a high branched chain amino acid digestion-free corn protein product, which takes corn protein powder as a raw material and is a digestion-free corn protein product containing high branched chain amino acid and finished by enzyme engineering, and the digestion-free corn protein product comprises the following components: the corn protein is a product mainly comprising composite amino acid with the average molecular weight of 120 daltons, small molecular dipeptide and tripeptide with the average molecular weight of 180 to 500 daltons, and the corn protein raw material contains about 22.24 percent of branched chain amino acid and has the highest content in vegetable protein, so the corn protein is used as the raw material to produce a digestion-free protein product with high branched chain amino acid.
2. The process adopts a membrane separation and purification method to separate out protein larger than 500Da to produce a high-purity and high-branched-chain amino acid digestion-free corn protein product, and can also produce the high-branched-chain amino acid digestion-free corn protein product without purification after enzymolysis.
3. The process adopts the treatment of more than 90 ℃ for more than 30 seconds, and needs to inactivate the protease so as to produce the enzyme inactive digestion-free protein product, and can also produce the product containing the protease activity without enzyme deactivation.
4. The feed liquid of the digestion-free protein of the high branched chain amino acid is concentrated and blended according to the standard of liquid products to produce liquid products, and the liquid products are concentrated, sprayed and dried to produce powdery products;
the preparation process comprises the following steps:
①, weighing the corn protein powder raw material;
②, mixing water (①) with the weight of 1: 8-10 times of the weight of the raw material into slurry;
③, carrying out ultra-high temperature sterilization and appropriate protein denaturation treatment on ② mixed slurry, wherein the temperature is at least 135 ℃ and needs to be kept for more than 50 seconds;
④, cooling ③ to reduce the temperature of the feed liquid to 54-56 ℃;
⑤, adding ④ into compound protease accounting for 0.3-0.5 percent of the weight of the raw materials for enzymolysis for 48-72 hours, wherein the pH value does not need to be adjusted in the enzymolysis process, but the raw materials need to be stirred slowly;
⑥, purifying the feed liquid after ⑤ enzymolysis by membrane separation, and carrying out enzyme deactivation treatment or enzyme deactivation treatment on the purified feed liquid at 90 ℃ for 30 seconds to produce a digestion-free corn protein product of high-purity, enzyme activity and inactive high-branched-chain amino acid;
⑦, carrying out enzymolysis on ⑤, and then carrying out enzyme deactivation treatment or enzyme deactivation treatment on the feed liquid at 90 ℃ for 30 seconds without membrane separation and purification to produce a digestion-free corn protein product with enzyme activity and inactive high-branched-chain amino acid;
⑧, concentrating the liquid of ⑥ and ⑦, blending according to the standard of the liquid product, and aseptically filling to obtain the liquid product;
⑨, concentrating ⑥, ⑦ and the feed liquid, and spray drying the solid matter to over 40% to obtain the powdery product.
5. The method of claim 1, 2, 3, or 4 for producing a highly branched chain amino acid digestion-free corn protein product, wherein the method comprises the steps of: taking zein as a raw material, and obtaining the digestion-free zein liquid and powder products of high purity or non-high purity, enzymatic activity and non-activity and high branched chain amino acid by pulp mixing, enzymolysis, membrane separation or non-membrane separation, and enzyme deactivation or non-enzyme deactivation.
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