CN109601695A - A kind of method that activated protein exempts from digesting protein with soybean protein for raw material preparation - Google Patents
A kind of method that activated protein exempts from digesting protein with soybean protein for raw material preparation Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of fertilized eggs egg be albumen through hatching live embryo and soybean protein is raw material, living body embryo and soybean protein mix to be crushed to size mixing and sterilize ultra-long time enzymatic hydrolysis and separate removal insoluble matter, and obtain tool placenta activity double protein exempts from digesting protein product.The product is made of dipeptides tri-peptide molecule amount in the compound amino acid of 300DA to 500DA and average molecular weight in 120DA.Science has proven to only this part and is only exempt from digesting protein, directly can be directly absorbed by the body utilization by small intestine.No digesting protein product is not exempted from discovery currently on the market, is exempted from digesting protein product the medicine have the advantages that providing for the people that the elderly's digestive function due to caused by aging reduces, is guaranteed nitrogen equilibrium.The vaccination of health revolution first time smallpox, the second penicillin control disease, third that virus and bacteria causes and utilize small active peptides treatment three times in mankind's history；All roots of all major diseases are exactly cell failure problems.
Description
Technical field
The present invention relates to a kind of biological food of enzyme engineering technology production, specifically a kind of activated protein and soybean egg
The white method for exempting from digesting protein for raw material preparation.Exempt from digesting protein be by dipeptides tri-peptide molecule amount in 300DA to 500DA and
Compound amino acid of the average molecular weight in 120DA forms.
Background technique
Amino acid is the basic unit of constitutive protein matter, and guarantees one of the important substance of human homergy.Human body
The amino acid that can be digested and assimilated only has 20 kinds.Wherein having 9 kinds of amino acid (baby is 10 kinds) cannot be synthesized or close in adult body
The needs of body it are not able to satisfy at speed, referred to as necessary amino acid, it must be supplemented from diet.Scientist thought only have in the past
Amino acid can be absorbed by organisms utilization by small intestine, mention so developing and exempting from the nutritional supplements of the various amino acid of digesting protein
It supplies patient to use, and most of is that the product that biotechnology synthesizes is expensive.But scientist has found small point with the development of science and technology
Sub- dipeptides tripeptides can also be directly absorbed and utilized by small intestine.This invention is exactly to be provided for society with biological enzymolysis technology containing small point
Sub- activity dipeptides, tripeptides and compound amino acid, also exempt from digesting protein product containing embryo viability.
The existing amino acid products for exempting from digesting protein, the obtained amino acid of synthesis generally produced with chemical synthesis is all
It is DL type amino acid, at high cost, reaction is complicated, and step is more, and by-product is more.The many amino acid whole quilts of other methods such as acid-base method
It destroys, and generates racemization.Fermentation method equipment investment is big, and production concentration is low, production cycle long uncomfortable industrialization.
The purpose of the present invention
Newest human senility theory thinks human body due to aging, and human body causes human body using endogenous protein deficiency
It is to cause one of body aging main cause that internal nitrogen source is unbalance.The daily small intestine of normal human will fall off 250 grams or so of epithelial cell
Albumen, the epithelial cell worked hard averagely only have two day service life, and newborn cell generates its removal of bottom of old cell simultaneously
Old cell is substituted, these aged cells are digested as food, but it is through gastric acid denaturation treatment, protein molecular spiral winding
, protease does not reach the surface of protein, so this part cell protein of normal person digests just for a long time in small intestine lower end
It can be absorbed by the human body.Since aging digestive function reduces, digestive ferment decreased, and activity also reduces, and makes that the elderly's is interior
Property cell protein in source cannot be recycled sufficiently, and daily albumen more than half jin, which cannot supplement, causes nitrogen source unbalance, this is new
It was found that one of the elderly's aging main cause.At this moment you eat how much protein since degenerate your digestive function of body reduces, and obtain not
To digestion just let alone absorb.What if, for the elderly of aging, to provide good digesting protein product of exempting from be unique
The method for solving this health problem.
Peptide is a revolutionary product, it will lead 21 century human nutrition revolution.It undergoes in total since the dawn of human civilization
Health revolution three times is crossed, first time smallpox occurs, and solution is vaccination, and second with mould extract for treating due to viral thin
Microbial disease, third time are exactly all diseases for utilizing small active peptides to treat including all major diseases, that is,
Cell failure problems.
Scientist has found a kind of drug that can promote longevity, and is tested with small white mouse and obtained for the first time
Success.The drug of this mystery is exactly the mixture scientific name being made of leucine, isoleucine and valine these three amino acid
These three amino acid are added in the water that mouse drinks in " branched-chain amino acid " experiment, as a result, it has been found that their service life averagely reaches
869 days, and common small white mouse can only then survive about 774 days.That is, the life that " branched-chain amino acid " is small white mouse prolongs
12% has been grown while extending the service life, the biological respinse of series of active also has occurred in the body of small white mouse, as cell is able to
Absorb more energy, free radical reduction etc..Free radical is the hazardous compound generated in body oxidation reaction, has Strong oxdiative
Property, the tissue and cell of body can be damaged, and then cause chronic disease and aging effect.It can prove that this three kinds of amino acid can be with
The intracorporal free radical of animal is removed, by these variation in vivo, small white mouse seems dynamic, and Coordinating Muscle ability mentions
Gao Liao, life extend.It is a kind of particularly significant and effective nutritional supplement that researcher, which introduces " branched-chain amino acid ", it can be with
It helps organism to enhance muscle without any side effect naturally and obtains more energy, remove interior free yl effect.This
The left Ni Suoli professor of project researcher's Milan, ITA university grace, he points out invention amino acid supplements on this basis, then it
It can promote the well-being of mankind in future, have very great help to the elderly or patient.
Do not find this invention also currently on the market exempts from digesting protein product, which is by molecular weight in 300DA
Compound amino acid in 120DA of small molecule dipeptides, tripeptides and average molecular weight to 500DA (dalton) is formed.Upper interview
Having arrived small active peptides is a revolutionary product, treats all including all major diseases using small active peptides
Disease, that is, cell failure problems.How that mends peptide, and there are two types of one, edible small molecule dipeptides and tripeptides for mode.Two, it is
Peptide is squeezed into body by injecting method.Calculating dipeptides by 20 kinds of amino acid has 400 kinds, and tripeptides has to be eaten in 8000 kinds of combinatorial theorys
There are 8400 kinds of functions and effect with small active peptides.Country it is raw all can to press soybean peptide standard without the standard of food peptide now
Food peptide is produced, product can only absorb a little, and science, which has proven to human body, can only absorb the peptide of molecular weight once in 500DA.Soybean
The standard molecular weight of one superfine product of peptide is less than 2000DA and is greater than 80%, and there are also the generations of limiting amino acid, this is highly difficult.This hair
Bright difference be not limiting amino acid generation important molecule amount be less than 500DA be exactly to exempt from digesting protein product.Liberation army army
Bioengineering Research Institute, thing medical college professor Tang Guoying preside over complete soybean peptide imitate in vitro human stomach, trypsase experiment and
Foreign literature reports that result is completely the same, and this kind of product enters body cannot be digested substantially, and peptide surface produces a kind of protection
Film is no longer by enzyme effect.
The technical solution of invention is to use the fertilized eggs of egg to be hatched as live embryo and soybean protein double protein to be raw material, use
Biological enzymolysis technology reaches while saving embryo viability substance and low cost, containing small molecule active dipeptides tripeptides and again
That closes amino acid exempts from digesting protein product.Scientific experiment has proven to the active matter that living body embryonated egg body has a lot of life conversions
Matter is significant to human health.
Summary of the invention
Exempt from digesting protein production it is an object of the present invention to provide the high embryo viability albumen of fraction, based on soybean protein
Product.Predominantly student market provides the protein product quickly absorbed for eliminating fatigue with tension pressure.Raw material fertilized eggs egg
Embryo viability biology.This method be not required in enzymolysis process be afraid of amino acid generation, excessive enzymatic hydrolysis be existing peptide enterprise all
The thing of fearness controls bad product and soybean peptide national standard is just not achieved.The most fundamental problem is that human body can only absorb molecule
Measure 300DA-500DA dipeptides tripeptides.This method is not required to adjust pH value in enzymolysis process, and processing step is simple, at low cost, remain
Bioactive ingredients keep its nutrition, biological value high.
New human senility theory thinks to make due to aged human body insufficient using own endogenous albumen, causes human body
Nitrogen source is unbalance to cause body aging.The daily small intestine of the normal human 8 ounce epithelial cell albumen that will fall off have 249 grams, diligent
The epithelial cell of work averagely only has two day service life, and newborn cell results from the bottom of each villus, its removal and replacement
Old cell, these aged cells are digested as food, but it is not through gastric acid denaturation treatment, so this part of normal person is thin
Born of the same parents' albumen is digested for a long time in small intestine lower end, and be just absorbed by the body utilization.Human digestive function reduces due to aged, disappears
Change enzyme reducing, activity is also reducing.Recycle old man's endogenous cellular protein cannot sufficiently, it is daily excellent more than half jin
Matter albumen cannot supplement, and cause protein insufficient, this is one of the elderly's aging main cause.At this moment no matter you mend albumen more again
Matter is not all used, and cannot be digested just let alone be absorbed.What if, it is unique for providing good digesting protein product of exempting from
The method for solving this health problem.It is also the most important significance of this product exploitation.
Step of preparation process:
1., by taking fertilized eggs as an example, fertilization hatching egg is through artificial incubation (use incubator) Constant temperature hatch method, incubation temperature are as follows:
Hatch at a temperature of 37.8, fertile egg is made to be changed into chicken son's tire albumen mixing soya beans albumen living as double protein raw material, containing height
Active high nutrition ingredient.It is primary with candler candling through hatching the 5th day, remove no fertilized eggs；
2., pearl living will be hatched in 1 to except before young, using embryo's living body biological, to egg shell between hatching 17 to 19 days
Living body is removed after disinfection treatment, adding water to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%；
3., with a small amount of disinfectant mixing compound protease, i.e., allotment liquid of protease, compound enzyme dosage are 0.3% -0.5%；
4., step 2 and step 3 and soybean protein be mixed together, the ratio of embryo's albumen and soybean protein is substantially arrived 1/10
Between 2/10.(i.e. animal protein and plant albumen ratio between 1/10-2/10) afterwards heating, temperature control 54 DEG C to 56 DEG C it
Between be achieved that enzymatic hydrolysis.It mixes slowly simultaneously, enzymolysis time is between 68 hours to 72 hours, and entire enzymolysis process is not required to adjust PH
Value；
5., the feed liquid after the completion of step 4 enzymatic hydrolysis is carried out to the lysate of the isolated pure small peptide of seperator and compound amino acid；
6., step 5 is carried out to sterilization and destroy the enzyme treatment, feed liquid reaches 75 DEG C -90 DEG C in temperature through coil sterilizer, heat preservation
15 --- 16 seconds, reach sterilising and enzyme inactivating purpose；
7., by step 6 by double effect evaporator concentration material 38% --- after between 43%, be spray-dried to obtain and exempt to digest
Small-molecular peptides (containing compound amino acid) protein product.
The present invention has the advantages that:
The present invention provides the good protein product for really exempting from digestion for society.And provide the dipeptides for exempting to digest small molecule active
Kyrine product, science have confirmed that edible small-molecular peptides, only dipeptides, tripeptides just exempt to digest, and directly pass through intestinal mucosa and enter
Body.
Small-molecular peptides therapy will be improved at least human longevity 20 years.The most important substance of human body birth and old age, sickness and death is adjusted, if
Not having peptide, there will be no life presence.
Peptide is a revolutionary product, it will lead 21 century human nutrition revolution.It undergoes in total since the dawn of human civilization
Health revolution three times is crossed, first time smallpox occurs, and solution is vaccination, and second with mould extract for treating due to viral thin
Microbial disease, third time are exactly all diseases for utilizing small active peptides to treat including all major diseases, that is,
Cell failure problems.
The present invention also provides the compound amino acid protein products for exempting from digestion.Wherein contain strong body in compound amino acid
" branched-chain amino acid " produces " branched-chain amino acid " fluid infusion, will promote the well-being of mankind in future！Scientist has found that one kind being capable of macrobiosis benefit
The drug in longevity, and tested with small white mouse and succeeded for the first time.The drug of this mystery is exactly by leucine, different
It is added in the water that mouse drinks in mixture scientific name " branched-chain amino acid " experiment of leucine and these three amino acid of valine composition
These three amino acid, as a result, it has been found that their service life has averagely reached 869 days, and common small white mouse can only then survive about
774 days.That is, the life that " branched-chain amino acid " is small white mouse extends 12% while extending the service life, the machine of small white mouse
The biological respinse that series of active also has occurred in vivo, as cell is able to absorb more energy, free radical reduction etc..Free radical
It is the hazardous compound generated in body oxidation reaction, there is strong oxidizing property, the tissue and cell of body, Jin Eryin can be damaged
Play chronic disease and aging effect.It can prove that this three kinds of amino acid can remove the intracorporal free radical of animal, in vivo by these
Variation, small white mouse seems dynamic, and Coordinating Muscle ability improves, and life extends.Researcher introduces " branch
Amino acid " is a kind of particularly significant and effective nutritional supplement, it can help organism naturally without any side effect
Enhance muscle and obtain more energy, removes interior free yl effect.The left Buddhist nun's rope of this project researcher's Milan, ITA university grace
In teach, he points out invention amino acid supplements on this basis, then it can promote the well-being of mankind in future, has to the elderly or patient
Very big help.
New human senility theory thinks to make due to aged human body insufficient using own endogenous albumen, causes human body
Nitrogen source is unbalance to cause body aging.The daily small intestine of the normal human 8 ounce epithelial cell albumen that will fall off have 249 grams, diligent
The epithelial cell of work averagely only has two day service life, and newborn cell results from the bottom of each villus, its removal and replacement
Old cell, these aged cells are digested as food, but it is not through gastric acid denaturation treatment, so this part of normal person is thin
Born of the same parents' albumen is digested for a long time in small intestine lower end, and be just absorbed by the body utilization.Human digestive function reduces due to aged, disappears
Change enzyme reducing, activity is also reducing.Recycle old man's endogenous cellular protein cannot sufficiently, it is daily excellent more than half jin
Matter albumen cannot supplement, and cause protein insufficient, this is one of the elderly's aging main cause.At this moment no matter you mend albumen more again
Matter is not all used, and cannot be digested just let alone be absorbed.What if, it is unique for providing good digesting protein product of exempting from
The method for solving this health problem.It is also the most important significance of this product exploitation！
Specific embodiment
Embodiment 1
Living body embryonated egg 20kg adds 50kg soybean protein isolate.First the kind after black line hair removes no fertilized eggs is blocked with candle
Egg 20kg, each hatching egg is based on 45 grams, and about 444.Hatch in 37.8 DEG C of artificial incubation device, is tested again with candler within the 5th day
Egg is primary, chooses no fertilized eggs.
Between during hatching 17 to 19 days, living body is removed to after the disinfection treatment of egg shell within this example selected 18 days, gone
After falling stillborn foetus, adding water and soybean protein isolate mixing to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%.
With a small amount of disinfectant mixing compound protease, i.e. allotment liquid of protease, compound enzyme dosage is 0.3% -0.5%.This
75 grams of compound proteases are added by 0.3% allotment in secondary enzyme dosage.Then the protease of the protein raw materials and allotment that living body are crushed
Liquid mixing heating, temperature control which achieves enzymatic hydrolysis while mixing slowly, enzymolysis time arrives for 68 between 54 DEG C to 56 DEG C
Between 72 hours, this example 68 hours of selected enzymatic hydrolysis, entire enzymolysis process is not required to adjust pH value.
Hatching egg after this example 444 screenings removes 3 again after hatching five days and there is not fertile egg, and disinfection shelling also removes
2 stillborn foetuses, real 439 living body embryos in total, remove eggshell 3kg and 1kg non-living body egg, embryo 17kg embryo living body egg
White liquor.By 70% is aqueous plus water based on this example obtain 5.2kg, and 50kg protein isolate altogether in 55.2kg material protein addition 413.2kg
Water mixes embryo's albumen containing solid 12% feed liquid, through 68 hours enzymolysis processings.
Processing is centrifuged in feed liquid after enzymatic hydrolysis, large particulate matter is removed out, obtains fully dissolved and exempt from digesting protein
Liquid form product.
Exempt from digestible protein liquid to fully dissolved to be concentrated through double effect evaporator, is concentrated to containing granular type object 40% or more, is sprayed
Be dried to obtain powdery exempts from digesting protein product.
Example 2
Living body embryonated egg 40kg adds 50kg soybean protein isolate.First the kind after black line hair removes no fertilized eggs is blocked with candle
Egg 40kg, each hatching egg is based on 45 grams, and about 888.Hatch in 37.8 degree of artificial incubation device, is tested again with candler within the 5th day
Egg is primary, chooses no fertilized eggs.
Between during hatching 17 to 19 days, living body is removed to after the disinfection treatment of egg shell within this example selected 18 days, gone
After falling stillborn foetus, adding water and soybean protein isolate mixing to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%.
With a small amount of disinfectant mixing compound protease, i.e. allotment liquid of protease, compound enzyme dosage is 0.3% -0.5%.This
75 grams of compound proteases are added by 0.3% allotment in secondary enzyme dosage.Then the protease of the protein raw materials and allotment that living body are crushed
Liquid mixing heating, temperature control which achieves enzymatic hydrolysis while mixing slowly, enzymolysis time arrives for 68 between 54 DEG C to 56 DEG C
Between 72 hours, this example 68 hours of selected enzymatic hydrolysis, entire enzymolysis process is not required to adjust pH value.
Hatching egg after this example 888 screenings removes 6 again after hatching five days and there is not fertile egg, and disinfection shelling also removes
4 stillborn foetuses, real 878 living body embryos in total, remove eggshell 6kg and 2kg non-living body egg, embryo 34kg embryo living body egg
White liquor.By 70% is aqueous plus water based on this example obtain 10.4kg, and 50kg protein isolate altogether in 60.4kg material protein addition 443kg
Water mixes embryo's albumen containing solid 12% feed liquid, through 68 hours enzymolysis processings.
This example adds water 443kg, obtains the live fish albumen feed liquid containing solid 12%, then through ultra high temperature sterilization and albumen qualitative change
Property 135 DEG C -140 DEG C for the treatment of temperature after keep then be cooled to 54 DEG C of degree between 56 DEG C of degree in 15 seconds -20 seconds.
75 grams of compound protease are added by solid 0.3%.Temperature controls between 54 degree to 56, and which achieves enzymatic hydrolysis
It mixes slowly simultaneously, enzymolysis time is between 68 to 72 hours, this example 68 hours of selected enzymatic hydrolysis, entire enzymolysis process is not required to
Adjust pH value.
Processing is centrifuged in feed liquid after enzymatic hydrolysis, large particulate matter is removed out, obtains fully dissolved and exempt from digesting protein
Liquid form product.
Exempt from digestible protein liquid to fully dissolved to be concentrated through double effect evaporator, is concentrated to 40% or more solid content, is spray-dried
Obtain powdery exempts from digesting protein product.
Claims (3)
1. a kind of activated protein and soybean protein are the method that digesting protein is exempted from raw material preparation, it is characterised in that: activated protein
The biodegrade that it is fertilized eggs egg through hatching live embryo for protein raw materials by enzyme that original, which use, obtains having that placenta is active to exempt to digest
Protein product;The product by dipeptides tri-peptide molecule amount 300DA to 500DA and average molecular weight 120DA compounded amino
Acid composition;
Step of preparation process is:
1., by taking fertilized eggs as an example, fertilization hatching egg is through artificial incubation (use incubator) Constant temperature hatch method, incubation temperature are as follows:
Hatch at a temperature of 37.8, fertile egg is made to be changed into chicken son's tire albumen mixing soya beans albumen living as double protein raw material, containing height
Active high nutrition ingredient；It is primary with candler candling through hatching the 5th day, remove no fertilized eggs;
2., pearl living will be hatched in 1 to except before young, using embryo's living body biological, to egg shell between hatching 17 to 19 days
Living body is removed after disinfection treatment, adding water to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%;
3., with a small amount of disinfectant mixing compound protease, i.e., allotment liquid of protease, compound enzyme dosage are 0.3% -0.5%;
4., step 2 and step 3 and soybean protein be mixed together, the ratio of embryo's albumen and soybean protein is substantially arrived 1/10
Between 2/10；(i.e. animal protein and plant albumen ratio between 1/10-2/10) afterwards heating, temperature control 54 DEG C to 56 DEG C it
Between be achieved that enzymatic hydrolysis；It mixes slowly simultaneously, enzymolysis time is between 68 hours to 72 hours, and entire enzymolysis process is not required to adjust PH
Value;
5., the feed liquid after the completion of step 4 enzymatic hydrolysis is carried out to the lysate of the isolated pure small peptide of seperator and compound amino acid;
6., step 5 is carried out to sterilization and destroy the enzyme treatment, feed liquid reaches 75 DEG C -90 DEG C in temperature through coil sterilizer, heat preservation
15-16 seconds, reach sterilising and enzyme inactivating purpose;
7., by step 6 by double effect evaporator concentration material 40% or more, be spray-dried to obtain exempt from digest small-molecular peptides
(containing compound amino acid) protein product.
2. the preparation method of digesting protein is exempted from a kind of activated protein original preparation according to claim 1, feature exists
In: activated protein is former;A kind of fertilized eggs egg is used, for protein raw materials (containing living fry), to save embryo viability through hatching live embryo
Substance;Enzymatic hydrolysis obtains tool placenta is active exempts from digesting protein product for a long time;The product is by dipeptides tri-peptide molecule amount in 300DA
Compound amino acid to 500DA and average molecular weight in 125DA forms.
3. uncomfortable ph value freely digests in technique, the temperature for carrying out sterilization and enzyme deactivation controls the heat preservation 15 at 75 DEG C -90 DEG C ---
16 seconds, that does not destroy biological embryo viability exempted from digestible protein powder product.
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Cited By (3)
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| CN110050874A (en) * | 2019-04-18 | 2019-07-26 | 长春大学 | A kind of method that soybean biochemistry proliferin matter exempts from digesting protein for raw material preparation |
| CN110100897A (en) * | 2019-04-22 | 2019-08-09 | 长春大学 | A kind of preparation method of small-molecular peptides milk and small-molecular peptides milk powder |
| CN111772031A (en) * | 2020-06-09 | 2020-10-16 | 讷河市笙得利农业科技发展有限公司 | Device and method for preparing digestion-free protein by taking active protein and soybean protein as raw materials |
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| CN111772031A (en) * | 2020-06-09 | 2020-10-16 | 讷河市笙得利农业科技发展有限公司 | Device and method for preparing digestion-free protein by taking active protein and soybean protein as raw materials |
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