CN109385458A - A kind of method that activated protein exempts from digesting protein for raw material preparation - Google Patents
A kind of method that activated protein exempts from digesting protein for raw material preparation Download PDFInfo
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 56
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- 238000002360 preparation method Methods 0.000 title claims description 7
- 235000013601 eggs Nutrition 0.000 claims abstract description 26
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 23
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 16
- 230000012447 hatching Effects 0.000 claims abstract description 14
- 108010016626 Dipeptides Proteins 0.000 claims abstract description 13
- -1 compound amino acid Chemical class 0.000 claims abstract description 12
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 11
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 11
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 9
- 210000002826 placenta Anatomy 0.000 claims abstract 3
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- 102000004190 Enzymes Human genes 0.000 claims description 14
- 108090000790 Enzymes Proteins 0.000 claims description 14
- 108091005804 Peptidases Proteins 0.000 claims description 10
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- 150000001875 compounds Chemical class 0.000 claims description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 9
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- 102000035195 Peptidases Human genes 0.000 description 1
- 208000008425 Protein deficiency Diseases 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
It is that the sterilized shelling crushing of protein raw materials (contain living fry) sizes mixing and sterilizes ultra-long time enzymatic hydrolysis separation removal insoluble matter that the invention discloses a kind of fertilized eggs eggs through hatching live embryo, obtains having that placenta is active to exempt from digesting protein product.The product is made of dipeptides tri-peptide molecule amount in the compound amino acid of 300DA to 500DA and average molecular weight in 125DA.Science has proven to only this part and is only exempt from digesting protein, directly can be directly absorbed by the body utilization by small intestine.No digesting protein product is not exempted from discovery currently on the market, the medicine have the advantages that provide for the elderly and the bad people of digestive function and exempt from digesting protein product, guarantee nitrogen equilibrium.The vaccination of health revolution first time smallpox, second of penicillin control the disease of virus and bacteria initiation, utilize small active peptides treatment for the third time three times in mankind's history.All roots of all major diseases are exactly cell failure problems.
Description
Technical field
The present invention relates to a kind of biological food of enzyme engineering technology production, specifically a kind of activated protein is raw material system
The standby method for exempting from digesting protein.Exempting from digesting protein is by dipeptides tri-peptide molecule amount in 300DA to 500DA and average molecular weight
It is formed in the compound amino acid of 120DA.
Background technique
Amino acid is the basic unit of constitutive protein matter, and guarantees one of the important substance of human homergy.Human body
The amino acid that can be digested and assimilated only has 20 kinds.Wherein having 9 kinds of amino acid (baby is 10 kinds) cannot be synthesized or close in adult body
The needs of body it are not able to satisfy at speed, referred to as necessary amino acid, it must be supplemented from diet.Scientist thought only have in the past
Amino acid can be absorbed by organisms utilization by small intestine, mention so developing and exempting from the nutritional supplements of the various amino acid of digesting protein
It supplies patient to use, and most of is that the product that biotechnology synthesizes is expensive.But scientist has found small point with the development of science and technology
Sub- dipeptides tripeptides can also be directly absorbed and utilized by small intestine.This invention is exactly to be provided for society with biological enzymolysis technology containing small point
Sub- activity dipeptides, tripeptides and compound amino acid, also exempt from digesting protein product containing embryo viability.
The existing amino acid products for exempting from digesting protein, the obtained amino acid of synthesis generally produced with chemical synthesis is all
It is DL type amino acid, at high cost, reaction is complicated, and step is more, and by-product is more.The many amino acid whole quilts of other methods such as acid-base method
It destroys, and generates racemization.Fermentation method equipment investment is big, and production concentration is low, production cycle long uncomfortable industrialization.
The purpose of the present invention
Newest human senility theory thinks human body due to aging, and human body causes human body using endogenous protein deficiency
It is to cause one of body aging main cause that internal nitrogen source is unbalance.The daily small intestine of normal human will fall off 250 grams or so of epithelial cell
Albumen, the epithelial cell worked hard averagely only have two day service life, and newborn cell generates its removal of bottom of old cell simultaneously
Old cell is substituted, these aged cells are digested as food, but it is through gastric acid denaturation treatment, protein molecular spiral winding
, protease does not reach the surface of protein, so this part cell protein of normal person digests just for a long time in small intestine lower end
It can be absorbed by the human body.Since aging digestive function reduces, digestive ferment decreased, and activity also reduces, and makes that the elderly's is interior
Property cell protein in source cannot be recycled sufficiently, and daily albumen more than half jin, which cannot supplement, causes nitrogen source unbalance, this is new
It was found that one of the elderly's aging main cause.At this moment you eat how much protein since degenerate your digestive function of body reduces, and obtain not
To digestion just let alone absorb.What if, for the elderly of aging, to provide good digesting protein product of exempting from be unique
The method for solving this health problem.
Peptide is a revolutionary product, it will lead 21 century human nutrition revolution.It undergoes in total since the dawn of human civilization
Health revolution three times is crossed, first time smallpox occurs, and solution is vaccination, and second with mould extract for treating due to viral thin
Microbial disease, third time are exactly all diseases for utilizing small active peptides to treat including all major diseases, that is,
Cell failure problems.
Scientist has found a kind of drug that can promote longevity, and is tested with small white mouse and obtained for the first time
Success.The drug of this mystery is exactly the mixture scientific name being made of leucine, isoleucine and valine these three amino acid
These three amino acid are added in the water that mouse drinks in " branched-chain amino acid " experiment, as a result, it has been found that their service life averagely reaches
869 days, and common small white mouse can only then survive about 774 days.That is, the life that " branched-chain amino acid " is small white mouse prolongs
12% has been grown while extending the service life, the biological respinse of series of active also has occurred in the body of small white mouse, as cell is able to
Absorb more energy, free radical reduction etc..Free radical is the hazardous compound generated in body oxidation reaction, has Strong oxdiative
Property, the tissue and cell of body can be damaged, and then cause chronic disease and aging effect.It can prove that this three kinds of amino acid can be with
The intracorporal free radical of animal is removed, by these variation in vivo, small white mouse seems dynamic, and Coordinating Muscle ability mentions
Gao Liao, life extend.It is a kind of particularly significant and effective nutritional supplement that researcher, which introduces " branched-chain amino acid ", it can be with
It helps organism to enhance muscle without any side effect naturally and obtains more energy, remove interior free yl effect.?
Invention amino acid supplements on the basis of this have very great help to the elderly or patient then it can promote the well-being of mankind in future.
Do not find this invention also currently on the market exempts from digesting protein product, which is by molecular weight in 300DA
Compound amino acid in 120DA of small molecule dipeptides, tripeptides and average molecular weight to 500DA (dalton) is formed.Upper interview
Having arrived small active peptides is a revolutionary product, treats all including all major diseases using small active peptides
Disease, that is, cell failure problems.How that mends peptide, and there are two types of modes: one, eating small molecule dipeptides and tripeptides.Two, it is
Peptide is squeezed into body by injecting method.Calculating dipeptides by 20 kinds of amino acid has 400 kinds, and tripeptides has to be eaten in 8000 kinds of combinatorial theorys
There are 8400 kinds of functions and effect with small active peptides.Country it is raw all can to press soybean peptide standard without the standard of food peptide now
Food peptide is produced, product can only absorb a little, and science, which has proven to human body, can only absorb the peptide of molecular weight once in 500DA.Soybean
The standard molecular weight of one superfine product of peptide is less than 2000DA and is greater than 80%, and there are also the generations of limiting amino acid, this is highly difficult.This hair
Bright difference be not limiting amino acid generation important molecule amount be less than 500DA be exactly to exempt from digesting protein product.This kind of product
It cannot be digested substantially into body, peptide surface produces a kind of protective film no longer by enzyme effect.
The technical solution of invention is that be hatched using the fertilized eggs of egg be protein raw materials for live embryo be raw material, with life
Object zymolysis technique reaches while saving embryo viability substance and low cost, contains small molecule active dipeptides tripeptides and compound
Amino acid exempts from digesting protein product.Scientific experiment has proven to the active material that living body embryonated egg body has a lot of life conversions,
It is significant to human health.So the present invention exempts from digesting protein product using the substance as what raw material produced high activity.
Summary of the invention
It is an object of the present invention to provide a kind of high embryo viability, high biological values to exempt from digesting protein product.Raw material with by
The embryo viability biology of the ovum egg of essence.This method is not required to be afraid of the generation of amino acid in enzymolysis process, and excessive enzymatic hydrolysis is existing
The thing that peptide enterprise is all afraid of controls bad product and soybean peptide national standard is just not achieved.The most fundamental problem is that human body can only
Absorb molecular weight 300DA-500DA dipeptides tripeptides.This method is not required to adjust pH value in enzymolysis process, processing step is simple, cost
It is low, by living body embryo proteinogen, remain many bioactive ingredients, keep its nutrition, biological value high.
Step of preparation process:
1., by taking fertilized eggs as an example, fertilization hatching egg is through artificial incubation (use incubator) Constant temperature hatch method, incubation temperature are as follows:
Hatch at a temperature of 37.8, fertile egg is made to be changed into chicken son's tire protein raw materials living, the ingredient of high nutrition containing high activity.Through hatching the
Five days primary with candler candling, removes no fertilized eggs.
2., pearl living will be hatched in 1 to except before young, using embryo's living body biological, to egg between hatching 17 to 19 days
Living body is removed after shell disinfection treatment, adding water to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%.
3., with a small amount of disinfectant mixing compound protease, i.e., allotment liquid of protease, compound enzyme dosage are 0.3%-
0.5%。
4., 2. 3. step is mixed to heating, temperature control with step between 54 DEG C to 56 DEG C, it is same which achieves enzymatic hydrolysis
When mix slowly, enzymolysis time be 68 to 72 hours between, entire enzymolysis process be not required to adjust pH value.
5., 4. step is digested after the completion of feed liquid carry out the dissolution of the isolated pure small peptide of seperator and compound amino acid
Liquid.
6., 5. step is carried out to sterilization and destroy the enzyme treatment, feed liquid reaches 75 DEG C -90 DEG C in temperature through coil sterilizer, protects
Temperature 15 --- 16 seconds, reach sterilising and enzyme inactivating purpose.
7., by step 6 by double effect evaporator concentration material 38% --- after between 43%, be spray-dried and exempted from
Digest small-molecular peptides (containing compound amino acid) protein product.
The present invention has the advantages that:
The present invention provides the good protein product for really exempting from digestion for society.And provide the dipeptides for exempting to digest small molecule active
Kyrine product, science have confirmed that edible small-molecular peptides, only dipeptides, tripeptides just exempt to digest, and directly pass through intestinal mucosa and enter
Body.
Hereby doctor says famous American biologist carat: small-molecular peptides therapy will be improved at least human longevity 20 years.It is modern
Scientific forefront adjusts the most important substance of human body birth and old age, sickness and death, if there will be no life presence without peptide.
The present invention also provides the compound amino acid protein products for exempting from digestion.Wherein contain strong body in compound amino acid
Left professor Ni Suoli of the grace of " branched-chain amino acid " Milan, ITA university points out: production " branched-chain amino acid " fluid infusion, will be in future
It promotes the well-being of mankind.
Scientist has found a kind of drug that can promote longevity, and is tested with small white mouse and obtained for the first time
Success.The drug of this mystery is exactly the mixture scientific name being made of leucine, isoleucine and valine these three amino acid
These three amino acid are added in the water that mouse drinks in " branched-chain amino acid " experiment, as a result, it has been found that their service life averagely reaches
869 days, and common small white mouse can only then survive about 774 days.That is, the life that " branched-chain amino acid " is small white mouse prolongs
12% has been grown while extending the service life, the biological respinse of series of active also has occurred in the body of small white mouse, as cell is able to
Absorb more energy, free radical reduction etc..Free radical is the hazardous compound generated in body oxidation reaction, has Strong oxdiative
Property, the tissue and cell of body can be damaged, and then cause chronic disease and aging effect.It can prove that this three kinds of amino acid can be with
The intracorporal free radical of animal is removed, by these variation in vivo, small white mouse seems dynamic, and Coordinating Muscle ability mentions
Gao Liao, life extend.It is a kind of particularly significant and effective nutritional supplement that researcher, which introduces " branched-chain amino acid ", it can be with
It helps organism to enhance muscle without any side effect naturally and obtains more energy, remove interior free yl effect.?
Invention amino acid supplements on the basis of this have very great help to the elderly or patient then it can promote the well-being of mankind in future.
New human senility theory thinks to make due to aged human body insufficient using own endogenous albumen, causes human body
Nitrogen source is unbalance to cause body aging.The daily small intestine of the normal human 8 ounce epithelial cell albumen that will fall off have 249 grams, diligent
The epithelial cell of work averagely only has two day service life, and newborn cell results from the bottom of each villus, its removal and replacement
Old cell, these aged cells are digested as food, but it is not through gastric acid denaturation treatment, so this part of normal person is thin
Born of the same parents' albumen is digested for a long time in small intestine lower end, and be just absorbed by the body utilization.Human digestive function reduces due to aged, disappears
Change enzyme reducing, activity is also reducing.Recycle old man's endogenous cellular protein cannot sufficiently, it is daily excellent more than half jin
Matter albumen cannot supplement, and cause protein insufficient, this is one of the elderly's aging main cause.At this moment no matter you mend albumen more again
Matter is not all used, and cannot be digested just let alone be absorbed.What if, it is unique for providing good digesting protein product of exempting from
The method for solving this health problem.It is also the most important significance of this product exploitation.
Specific embodiment
Embodiment 1
The hatching egg 100kg after black line hair removes no fertilized eggs first is blocked with candle, each hatching egg is based on 45 grams, and about 2222
It is a.Hatch in 37.8 degree of artificial incubation device, the 5th day primary with candler candling again, chooses no fertilized eggs.
Between during hatching 17 to 19 days, living body is removed to after the disinfection treatment of egg shell within this example selected 18 days, gone
After falling stillborn foetus, adding water to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%.
With a small amount of disinfectant mixing compound protease, i.e. allotment liquid of protease, compound enzyme dosage is 0.3% -0.5%.This
75 grams of compound proteases are added by 0.3% allotment in secondary enzyme dosage.Then the protease of the protein raw materials and allotment that living body are crushed
Liquid mixing heating, temperature control which achieves enzymatic hydrolysis while mixing slowly, enzymolysis time arrives for 68 between 54 DEG C to 56 DEG C
Between 72 hours, this example 68 hours of selected enzymatic hydrolysis, entire enzymolysis process is not required to adjust pH value.
Hatching egg after this example 2222 screenings removes 13 again after hatching five days and there is not fertile egg, and disinfection shelling is also gone
In addition to 9 stillborn foetuses, reality obtains 2200 living body embryos in total, removes eggshell 15kg and 1kg non-living body egg, obtains embryo 84kg embryo work
Body protein liquid.By 70% is aqueous plus water based on this example obtain 25.2kg, 125kg water is added in 84kg embryo liquid and mixes embryonated egg
The feed liquid of Bai Hangu 12%, through 68 hours enzymolysis processings.
Processing is centrifuged in feed liquid after enzymatic hydrolysis, large particulate matter is removed out, obtains fully dissolved and exempt from digesting protein
Liquid form product.
Exempt from digestible protein liquid to fully dissolved to be concentrated through double effect evaporator, is concentrated to 40% or more solid content, is spray-dried
Obtain powdery exempts from digesting protein product.
Example 2
Living fry 100kg, is cleaned plus hydrocolloid milling is broken, and fineness will pass through 100 mesh bolters.The internal of general fish aqueous be
70—80%。
This example adds water 125kg, obtains the live fish albumen feed liquid containing solid 12%, then through ultra high temperature sterilization and albumen qualitative change
Property treatment temperature 135-140 degree after keep 15 seconds-20 seconds then be cooled between 54 degree to 56 degree.
75 grams of compound protease are added by solid 0.3%.Temperature controls between 54 degree to 56 degree, and which achieves enzymes
It solves while mixing slowly, enzymolysis time is between 68 to 72 hours, this example 68 hours of selected enzymatic hydrolysis, entire enzymolysis process is not
PH value need to be adjusted.
Processing is centrifuged in feed liquid after enzymatic hydrolysis, large particulate matter is removed out, obtains fully dissolved and exempt from digesting protein
Liquid form product.
Exempt from digestible protein liquid to fully dissolved to be concentrated through double effect evaporator, is concentrated to 40% or more solid content, is spray-dried
Obtain powdery exempts from digesting protein product.
Claims (3)
1. a kind of activated protein is that the method for digesting protein is exempted from raw material preparation, it is characterised in that: activated protein original using be by
Smart ovum egg is protein raw materials (contain living fry) by the biodegrade of enzyme through hatching live embryo, obtains tool placenta is active to exempt to digest
Protein product；The product by dipeptides tri-peptide molecule amount 300DA to 500DA and average molecular weight 125DA compounded amino
Acid composition;
Step of preparation process is:
1., by taking fertilized eggs as an example, fertilization hatching egg is through artificial incubation (use incubator) Constant temperature hatch method, incubation temperature are as follows:
Hatch at a temperature of 37.8, fertile egg is made to be changed into chicken son's tire protein raw materials living, the ingredient of high nutrition containing high activity；Through hatching the
Five days primary with candler candling, removes no fertilized eggs;
2., pearl living will be hatched in 1 to except before young, using embryo's living body biological, to egg shell between hatching 17 to 19 days
Living body is removed after disinfection treatment, adding water to size mixing after low-temperature grinding processing makes it containing solid content between 12% to 15%;
3., with a small amount of disinfectant mixing compound protease, i.e., allotment liquid of protease, compound enzyme dosage are 0.3% -0.5%;
4., step 2 mixed into step 3 heating, temperature control between 54 DEG C to 56 DEG C, which achieves enzymatic hydrolysis simultaneously at a slow speed
Stirring, enzymolysis time are between 68 to 72 hours, and entire enzymolysis process is not required to adjust pH value;
5., the feed liquid after the completion of step 4 enzymatic hydrolysis is carried out to the lysate of the isolated pure small peptide of seperator and compound amino acid;
6., step 5 is carried out to sterilization and destroy the enzyme treatment, feed liquid reaches 75 DEG C -90 DEG C in temperature through coil sterilizer, heat preservation
15 --- 16 seconds, reach sterilising and enzyme inactivating purpose;
7., by step 6 by double effect evaporator concentration material in solid content 40% or more, be spray-dried and exempted from
Digest small-molecular peptides (containing compound amino acid) protein product.
2. the preparation method of digesting protein is exempted from a kind of activated protein original preparation according to claim 1, feature exists
In: activated protein is former；A kind of fertilized eggs egg is used, for protein raw materials (containing living fry), to save embryo viability through hatching live embryo
Substance；Enzymatic hydrolysis obtains tool placenta is active exempts from digesting protein product for a long time；The product is by dipeptides tri-peptide molecule amount in 300DA
Compound amino acid to 500DA and average molecular weight in 125DA forms.
3. uncomfortable ph value freely digests in technique, the temperature for carrying out sterilization and enzyme deactivation controls the heat preservation 15 at 75 DEG C -90 DEG C ---
16 seconds, biological embryo viability is not destroyed, molecular weight exempts from digesting protein product 500DA is below.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109824774A (en) * | 2019-03-18 | 2019-05-31 | 时代生物科技（深圳）有限公司 | A method of recycling high activity peptide |
| CN109864134A (en) * | 2019-03-22 | 2019-06-11 | 长春大学 | Soybean and peanut or single substance are the method that raw material produces small-molecular peptides milk and powder |
| CN109875052A (en) * | 2019-03-21 | 2019-06-14 | 长春大学 | A kind of three clean bodies prebiotics exempt from the preparation method of digestion mung bean protein peptide |
| CN110050874A (en) * | 2019-04-18 | 2019-07-26 | 长春大学 | A kind of method that soybean biochemistry proliferin matter exempts from digesting protein for raw material preparation |
| CN110100897A (en) * | 2019-04-22 | 2019-08-09 | 长春大学 | A kind of preparation method of small-molecular peptides milk and small-molecular peptides milk powder |
| CN110564799A (en) * | 2019-09-19 | 2019-12-13 | 长春大学 | Preparation method for producing enzyme activity digestion-free protein by using soybean protein isolate as raw material |
| CN111019992A (en) * | 2019-12-16 | 2020-04-17 | 长春大学 | Preparation method for producing digestion-free protein by using animal protein as raw material |
| CN111387336A (en) * | 2019-12-26 | 2020-07-10 | 长春大学 | Preparation method of high branched chain amino acid digestion-free corn protein product |
| CN111616371A (en) * | 2019-12-26 | 2020-09-04 | 长春大学 | Preparation method for producing digestion-free protein by using various egg white proteins as raw materials |
| CN113025677A (en) * | 2021-03-12 | 2021-06-25 | 长春大学 | Method for preparing digestion-free sea cucumber protein by taking sea cucumber as raw material |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1154802A (en) * | 1996-12-12 | 1997-07-23 | 三九企业集团湖北公司 | Enayme-decomposed whole egg product, preparation method and use thereof |
| CN102871146A (en) * | 2012-10-22 | 2013-01-16 | 江南大学 | Production process of digestible chick-embryo egg health product |
| CN105411874A (en) * | 2015-12-30 | 2016-03-23 | 耿威 | Chick embryo bioactive peptide, preparation method and applications |
| CN105646647A (en) * | 2015-12-30 | 2016-06-08 | 耿威 | Chick embryo polypeptide as well as preparation method and application of chick embryo polypeptide |
| CN107201390A (en) * | 2017-07-02 | 2017-09-26 | 常德至善生物科技有限公司 | A kind of method that stepwise discretization method hydrolysis soft-shelled turtle egg prepares high anti-oxidation active peptide liquid |
-
2018
- 2018-12-18 CN CN201811547078.XA patent/CN109385458A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1154802A (en) * | 1996-12-12 | 1997-07-23 | 三九企业集团湖北公司 | Enayme-decomposed whole egg product, preparation method and use thereof |
| CN102871146A (en) * | 2012-10-22 | 2013-01-16 | 江南大学 | Production process of digestible chick-embryo egg health product |
| CN105411874A (en) * | 2015-12-30 | 2016-03-23 | 耿威 | Chick embryo bioactive peptide, preparation method and applications |
| CN105646647A (en) * | 2015-12-30 | 2016-06-08 | 耿威 | Chick embryo polypeptide as well as preparation method and application of chick embryo polypeptide |
| CN107201390A (en) * | 2017-07-02 | 2017-09-26 | 常德至善生物科技有限公司 | A kind of method that stepwise discretization method hydrolysis soft-shelled turtle egg prepares high anti-oxidation active peptide liquid |
Non-Patent Citations (2)
| Title |
|---|
| 李利东,袁建兴,宓晓黎,成恒嵩: "不同孵化期的鸡胚蛋营养和功效成分研究" * |
| 王俊杰;赵燕;涂勇刚;罗序英;李建科;陈彰毅;邓文辉;: "受精蛋孵化期间理化性质及水溶性蛋白(肽)抗氧化活性" * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109824774A (en) * | 2019-03-18 | 2019-05-31 | 时代生物科技（深圳）有限公司 | A method of recycling high activity peptide |
| CN109875052A (en) * | 2019-03-21 | 2019-06-14 | 长春大学 | A kind of three clean bodies prebiotics exempt from the preparation method of digestion mung bean protein peptide |
| CN109864134A (en) * | 2019-03-22 | 2019-06-11 | 长春大学 | Soybean and peanut or single substance are the method that raw material produces small-molecular peptides milk and powder |
| CN110050874A (en) * | 2019-04-18 | 2019-07-26 | 长春大学 | A kind of method that soybean biochemistry proliferin matter exempts from digesting protein for raw material preparation |
| CN110100897A (en) * | 2019-04-22 | 2019-08-09 | 长春大学 | A kind of preparation method of small-molecular peptides milk and small-molecular peptides milk powder |
| CN110564799A (en) * | 2019-09-19 | 2019-12-13 | 长春大学 | Preparation method for producing enzyme activity digestion-free protein by using soybean protein isolate as raw material |
| CN111019992A (en) * | 2019-12-16 | 2020-04-17 | 长春大学 | Preparation method for producing digestion-free protein by using animal protein as raw material |
| CN111387336A (en) * | 2019-12-26 | 2020-07-10 | 长春大学 | Preparation method of high branched chain amino acid digestion-free corn protein product |
| CN111616371A (en) * | 2019-12-26 | 2020-09-04 | 长春大学 | Preparation method for producing digestion-free protein by using various egg white proteins as raw materials |
| CN113025677A (en) * | 2021-03-12 | 2021-06-25 | 长春大学 | Method for preparing digestion-free sea cucumber protein by taking sea cucumber as raw material |
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