CN111019992A - Preparation method for producing digestion-free protein by using animal protein as raw material - Google Patents
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- CN111019992A CN111019992A CN201911288443.4A CN201911288443A CN111019992A CN 111019992 A CN111019992 A CN 111019992A CN 201911288443 A CN201911288443 A CN 201911288443A CN 111019992 A CN111019992 A CN 111019992A
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Abstract
The invention discloses a method for producing digestion-free protein by using animal protein (mainly referring to lean meat and fish meat of animals) as raw materials. Indigestible proteins are small proteins with a molecular weight below 500Da (daltons). The components of the compound amino acid are compound amino acid with the average molecular weight of 120Da and small molecular dipeptide and tripeptide with the average molecular weight of 180-500 Da. The protein segment does not need to be digested by human body, and can directly enter the body through the small intestine of the human body to be absorbed and utilized. The quality of products in domestic peptide industry is good and bad, which means that the content of dipeptide and tripeptide is determined, because the peptides with the length of more than 500 daltons are not directly absorbed and utilized by human bodies, and the products of the process are basically not digested for the second time after being eaten by human bodies. The production line can adopt the membrane separation technology to produce high-purity digestion-free protein products, and can also produce digestion-free protein products with a small content of more than 500Da without adopting a purification process.
Description
Technical Field
The invention relates to a novel food of a protein enzyme deep processing technology, in particular to a digestion-free protein product which can be directly absorbed by the small intestine of a human body with the molecular weight below 500Da and is converted from macromolecular soybean protein through the shearing action of enzyme. The components of the compound amino acid are compound amino acid with the average molecular weight of 120Da and small molecular dipeptide and tripeptide with the average molecular weight of 180Da to 500 Da. The protein segment does not need to be digested by human body, and can directly enter the body through the small intestine of the human body to be absorbed and utilized. The quality of products in domestic peptide industry is good and bad, which means that the content of dipeptide and tripeptide is determined, because the peptides larger than 500Da are not directly absorbed and utilized by human body, and the products of the process have a layer of protective film which is basically not digested for the second time after being eaten by human body and can not be utilized.
Background
According to the latest theories of life: the main reason for aging of the old is considered to be insufficient recycling of endogenous cell protein, and the main reason for aging is the lack of body nitrogen source caused by long-term shortage. The theory considers that the life cycle of human intestinal cells is 43 hours, the life cycle of cells on the surface of the stomach is three days, the old cells are shed as new cells grow out, the cells are digested and utilized by the human body like food, and normal people have 8-inch two, namely 248.8 g of intestinal cell protein shed every day. Half a kilogram of optimal human body cell protein with the biological potency of 100 does not consider the desquamated cell protein of the stomach, the cell protein of the intestine is not entwined by gastric acid denaturation treatment molecules, is very tight and is not easy to digest, and the normal young people can completely recycle the protein after the long-time digestion at the lower end of the small intestine.
The old people can not completely recycle endogenous cell protein due to the aging of each function of the organism, especially the reduction of the secretion amount and the reduction of the activity of digestive enzyme, so that the long-term nitrogen source imbalance of the organism is caused. It is clear what the body's long-term protein deficiency is as a result, why old people lose muscle so badly that the body cells use their own muscle proteins for survival. The protein-deficient organism is in a state of vicious circle, the old has no way, the digestive function of the body is reduced, and the protein in the body is not digested, namely the protein is absorbed and utilized. And the protein is not digested and fermented in the intestinal tract to generate a plurality of toxins such as skatole, and the toxins are absorbed by the large intestine to hurt the body. It is known that the reason is that smelly fart is caused when people eat more meat, and the digestion-free protein product is the only method for solving the problem of protein deficiency of the old.
The indigestible protein is a protein with a molecular weight below 500 Da. Wherein the compound amino acid has an average molecular weight of 120Da and the small molecular dipeptide and tripeptide have molecular weights of 180-500 Da. Scientists have discovered during the study of genes that the human body has a substance that determines all functional activities of the human body's life, called peptides. He speaks: peptide is a revolutionary product that will lead the human nutritional revolution in the 21 st century. The human has been undergoing three health revolution since history, the first smallpox appeared, the solution was the vaccinium nikoense, the second penicillin was used to treat the disease caused by bacteria and virus, the third was the use of small molecule active peptide to treat all diseases including all major diseases, which was the problem of cell failure. Medical science states that only one disease of humans is cellular failure. Cell failure is due to 2 causes, 1, the cells are not nourished. (not getting the needed things) 2, cytotoxic overload. (unwanted material is obtained).
Scientists have found a substance capable of prolonging life and have experimented with in mice, which was successful for the first time, the magical substance was a mixture of 3 amino acids leucine, isoleucine and valine, known by the scientific name "branched chain amino acids". In the experimental process, researchers add the 3 amino acids into water drunk by the tested mice, and as a result, the lives of the 3 amino acids are prolonged by 12%, and a series of positive biological reactions occur in the bodies of the mice while the lives of the 3 amino acids are prolonged, for example, cells can absorb more energy, free radicals are reduced, and the like, the free radicals are harmful compounds generated in oxidation reactions of the bodies, have strong oxidizing property, can damage tissues and cells of the bodies, and further cause chronic diseases and aging reactions. Through these in vivo changes, the mice appear to be fully active and have improved muscle coordination. Researchers have described that "branched chain amino acids" are an important and effective nutritional supplement that helps the organism strengthen muscles or gain more energy naturally without any side effects. Researchers in this project point out that the amino acid nutritional supplement is invented on the basis of the formula, so that the amino acid nutritional supplement can benefit human beings in the future and is of great help to the health of the old or patients.
The technology is to produce a digestion-free protein product by using animal protein as a raw material through an enzyme technology, wherein branched chain amino acids (BACC) account for 17-18.33% of the protein. Therefore, the development of the product has great significance to health.
Disclosure of Invention
The method aims to solve the problem that the old people have insufficient protein due to insufficient recovery and utilization of in-vivo cell protein caused by aging of the body, the old product of protein powder is replaced by the biotechnology, a new product of the biotechnology with higher value is provided, the problem of protein imbalance of the old people is solved, the most important for human health is to balance nutrients and reduce body toxins, the protein product which is not digested is produced for the old people, and the method has great effect on the health of the old people.
The preparation process comprises the following steps:
①, weighing animal protein as raw material.
②, cutting ① raw materials into blocks, crushing, and mixing with water and pulp in a ratio of 1: 9-10 times.
③, sterilizing ② and mixing the pulp, and aging and modifying at least 100 deg.C for 15 min.
④, cooling ③, hydrolyzing the fat of the material containing fat to make the temperature of the material liquid between 15 ℃ and 40 ℃, and adding 0.2% -0.5% of lipase for processing for more than 6 hours.
⑤, heating ④ to 54-56 ℃, adding compound protease accounting for 0.3-0.5% of the weight of the raw materials for enzymolysis for 48-72 hours, wherein the pH value does not need to be adjusted in the enzymolysis process, but the raw materials need to be stirred slowly.
⑥, purifying the feed liquid after ⑤ enzymolysis by membrane separation, and carrying out enzyme deactivation treatment on the purified feed liquid at 90 ℃ for 30 seconds to produce a high-purity digestion-free protein product.
⑦, carrying out enzymolysis on ⑤, and then carrying out enzyme deactivation treatment on the feed liquid at 90 ℃ for 30 seconds without membrane separation and purification to produce a digestion-free protein product.
⑧ blending ⑥, ⑦ and the liquid after enzymolysis according to the liquid product standard, and aseptically filling to obtain the liquid product.
⑨ concentrating ⑥ and ⑦ and the liquid to obtain solid matter over 40%, and spray drying to obtain powder.
The invention has the following advantages and positive effects:
1. the enzyme engineering of biotechnology is utilized to upgrade and replace the traditional protein powder product into a digestion-free protein product. The upgraded and updated product has the function of absorption without digestion, and has great significance for the aged with digestive function decline and the people in a sick state, and the liberated military soldiers and students who can eliminate fatigue and recover physical strength as soon as possible, and the digestion-free protein product.
2. Branched chain amino acid products are provided and studies have shown that: the branched chain amino acid can make the cell take more energy, reduce free radical and resist oxidation. Free radicals are harmful compounds generated in oxidation reactions of the body, have strong oxidizing properties, and can damage tissues and cells of the body, thereby causing chronic diseases and aging reactions. The nutritional supplement containing the body-strengthening branch and the amino acid has great help for the health of the elderly or patients.
3. Provides a small molecule peptide product which is really free from digestion in the market. The small molecular peptide is a component of human immune cells, and can proliferate the human immune cells and activate the human immune cells after entering a body. American shake video: the whole process of killing cancer cells by immune cells in a human body is also realized by the principle of the existing immunotherapy that the immune cells are injected into the human body after being proliferated in vitro, so that the immune cells complete the task of killing the cancer cells, and the small molecular peptide has the effect of preventing and treating cancers. The peptide obtained by human body has only two ways, namely, the small molecular dipeptide and tripeptide with the edible molecular weight of 180 Da-500 Da. Secondly, the peptide product is injected into the body through injection. Insulin, for example, is the 51 peptide, which saves countless lives of diabetics worldwide.
Detailed Description
Example 1:
1. the fish meat weighed 100 kg.
2. Cutting fish protein material into blocks, crushing, mixing with 9-10 times of water and mixing.
3. The mixed material liquid is sterilized at ultra-high temperature, cured and denatured, and is kept at 100 ℃ for more than 15 minutes.
4. Cooling to 54-56 deg.c.
5. Adding 0.3-0.5% of compound protease by weight of raw materials for enzymolysis, wherein 0.3 kg of compound protease is added in the example, the enzymolysis time is 70 hours, the temperature in the enzymolysis process needs to be between 54-56 ℃, the pH value does not need to be adjusted in the enzymolysis process, but the slow stirring treatment is needed.
6. Separating the enzymolysis feed liquid by a membrane to remove indigestible protein larger than 500Da, and carrying out enzyme deactivation treatment on the feed liquid for 30 seconds at 90 ℃ after purification to obtain a high-purity indigestible protein product.
7. And (4) blending, aseptically filling and processing the liquid product after membrane separation and enzyme deactivation according to the standard of the liquid product.
8. The feed liquid after membrane separation and enzyme deactivation is concentrated by a double-effect evaporator to a solid content of more than 40 percent, and then spray drying is carried out to obtain a powdery product.
Example 2:
1. beef weighs 200 kg.
2. Cutting beef raw materials into blocks, crushing, and mixing with 9-10 times of water.
3. The mixed material liquid is sterilized at ultra-high temperature, cured and denatured, and is kept at the temperature of at least 100 ℃ for more than 15 minutes.
4. Cooling to 15-40 deg.C (4.2% fat content of beef) to hydrolyze the fat into fatty acid, diglyceride, monoglyceride and glycerol. Add 0.2% -0.5% lipase, run 2 add 25 grams lipase. After 6 hours of treatment, the feed liquid is heated to 54-56 ℃ for proteolysis.
5. Adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, and adding 0.6 kg of compound protease for 70 hours in test 2, wherein the pH value is not required to be adjusted in the enzymolysis process, but the slow stirring treatment is required.
6. The enzymolysis feed liquid is subjected to enzyme deactivation treatment at 90 ℃ for 30 seconds after membrane separation and purification and without membrane separation and purification. Obtaining high-purity and non-high-purity digestion-free protein feed liquid products taking animal protein as raw materials.
7. And (3) blending the feed liquid according to the standard of the liquid product, and performing aseptic filling to obtain the high-purity and non-high-purity digestion-free protein liquid product.
8. Concentrating the feed liquid by a double-effect evaporator to a solid content of more than 40%, and then carrying out spray drying to obtain a high-purity and non-high-purity digestion-free protein powder product.
Claims (6)
1. A method for preparing digestion-free protein from animal protein (mainly lean meat and fish meat of animal) is provided; the digestion-free protein is an upgraded and updated product of conventional protein, and the components of the digestion-free protein mainly comprise composite amino acid with the average molecular weight of 120Da and products with micromolecule dipeptide and tripeptide of 180-500 Da; the method takes animal protein (mainly refers to lean meat and fish meat of animals) as raw materials, cuts off macromolecular protein through enzyme engineering, and achieves a protein product which can be directly absorbed and utilized by human bodies without digestion; the process adopts a membrane separation and purification method to separate out protein larger than 500Da, and produces a high-purity digestion-free protein product.
2. The process can also produce a digestion-free protein product which is mainly 500Da and has no high purity compared with the membrane process by enzymolysis without adopting a membrane separation technology.
3. The process adopts the treatment of more than 90 ℃ for more than 30 seconds, and protease needs to be inactivated, thereby achieving the purpose of producing the inactive digestion-free protein product.
4. The feed liquid after the digestion-free protein enzymolysis is prepared according to the liquid product standard, and a liquid product can be produced.
5. The powdery product can be produced by concentration and spray drying;
the preparation process comprises the following steps:
①, weighing the animal protein meat as raw materials;
②, cutting ① raw materials into blocks, crushing, and carrying out water and size mixing treatment according to the weight ratio of 1 to 9-10 times;
③, performing ultra-high temperature sterilization, aging and protein denaturation treatment on ② mixed slurry, and keeping the temperature at 100 ℃ for more than 15 minutes;
④, cooling ③ to reduce the temperature of the feed liquid to 15-40 ℃ to carry out fat hydrolysis treatment on the material containing fat, wherein the addition amount of lipase is 0.2-0.5 percent of the weight of the raw material, the treatment time is more than 6 hours, and the pH value does not need to be adjusted in the enzymolysis process, but the slow stirring treatment is needed;
⑤, heating ④ lipase to 54-56 ℃, adding 0.3-0.5% of compound protease for enzymolysis for 48-72 hours, wherein the pH value is not required to be adjusted in the enzymolysis process, but the mixture is slowly stirred;
⑥, purifying the feed liquid after ⑤ enzymolysis by membrane separation, and carrying out enzyme deactivation treatment on the purified feed liquid at 90 ℃ for 30 seconds to produce a high-purity digestion-free protein product;
⑦, carrying out enzymolysis on ⑤, purifying the feed liquid without membrane separation, and carrying out enzyme deactivation treatment on the feed liquid at 90 ℃ for 30 seconds to produce a digestion-free protein isolate product;
⑧, blending ⑥, ⑦ and the feed liquid according to the liquid product standard, and carrying out aseptic filling to obtain a liquid product;
⑨, concentrating ⑥, ⑦ and the feed liquid, and spray drying the solid matter to over 40% to obtain the powdery product.
6. The method for preparing the indigestible-free protein by using the animal protein as the raw material according to the claims 1, 2 and 3, which is characterized in that: the method takes animal protein as a raw material, and obtains high-purity and non-high-purity digestion-free protein liquid and powder products by dicing, crushing, size mixing, enzymolysis, membrane separation or non-membrane separation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911288443.4A CN111019992A (en) | 2019-12-16 | 2019-12-16 | Preparation method for producing digestion-free protein by using animal protein as raw material |
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| CN112868885A (en) * | 2021-03-12 | 2021-06-01 | 长春大学 | Method for preparing digestion-free silkworm pupa pit protein by using silkworm pupas as raw materials |
| CN112970931A (en) * | 2021-03-12 | 2021-06-18 | 长春大学 | Method for preparing digestion-free sea cubilose protein by using cubilose as raw material |
| CN113025677A (en) * | 2021-03-12 | 2021-06-25 | 长春大学 | Method for preparing digestion-free sea cucumber protein by taking sea cucumber as raw material |
| CN113892655A (en) * | 2021-10-12 | 2022-01-07 | 长春大学 | Method for preparing digestion-free whey protein by taking whey protein powder as raw material |
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