CN113841783A - Method for preparing digestion-free oyster protein by taking oysters as raw materials - Google Patents

Abstract

The invention discloses a method for preparing digestion-free oyster protein by taking oysters as raw materials, which belongs to food. The digestion-free protein is small-molecular protein with the molecular weight of below 500Da, and comprises composite amino acid with the average molecular weight of 120Da, small-molecular dipeptide and tripeptide with the molecular weight of 180-500 Da. The oyster is rich in protein and is named as seabed milk. All proteins are digested to be absorbed by human body and can be absorbed and utilized by human body. Because the digestive function of the old is reduced in declining secreted enzyme and activity along with the increase of the age, the utilization rate of the protein is reduced, and a lot of protein resources are lost and wasted, the development of the digestion-free protein product has great significance for supplementing insufficient protein nutrition for the old.

Description

Method for preparing digestion-free oyster protein by taking oysters as raw materials

Technical Field

The invention relates to a preparation method of food, in particular to a method for preparing digestion-free oyster protein by taking oysters as raw materials.

Background

According to the latest theories of life and physiology: the human body is in the growth period before the age of 25, is in the growth and maturity period from the age of 25 to the age of 30, can begin to decline in body functions after the age of 30, and has much reduced functions after the age of 60. The new theory suggests that the main reason of aging of the old people is that endogenous cellular protein can not be completely recycled, and the long-term shortage of body protein is the main reason of premature aging of the old people. The physiology states that: the life cycle of human intestinal cells is 43 hours at most, the life cycle of cells on the surface of the stomach is three days, old cells are exfoliated as new cells grow out, the cells are digested and utilized by the human body like food, and normal people have 8-inch and two-inch exfoliated cells, namely 248.8 g of intestinal cell proteins are exfoliated every day. Half jin of human body cell protein with the biological value of 100 is the best quality, the falling cell protein of the stomach is not considered, the cell protein of the small intestine is not denatured by gastric acid, the molecule winding is very tight and is not easy to digest, and the normal young people can completely recycle the protein after the long-time digestion at the lower end of the small intestine.

The human protein supply consists of three parts:

the first part supplies: is cell protein dropped from intestinal tract, the dropped cell is digested and utilized like food, adult has at least 8 engbi and 31.1 g per engbi every day, namely 248.8 g of dropped cell protein, the cell protein is human protein, is the best quality protein with 100 biological potency, and the biological potency of any other kind of protein is lower than the best quality protein.

A second part supplying: is dead cells in tissues, about 198-283 g of dead cells are repeatedly used by autophagy of human bodies every day, and autophagy means that the dead cells eat themselves. The existence of enzymes in living human cells that digest proteins, carbohydrates and fats can digest apoptotic cells for reuse by the cells is called autophagy. Looking up "one day hungry secret" autophagy science on the web, doctor university swedish japan scientist in 2016 thus won the nobel prize.

The third part supplies: is protein in food, and is supplemented according to 1.g-1.5 g per kilogram of body weight every day. The protein in food is digested in stomach, the protease secreted by human stomach is inactive and activated by gastric acid to digest the protein in food, and the aging of human body is the decrease of secretion in body which directly affects the digestion of protein in body.

The old people can not completely recycle endogenous cell protein due to the aging of each function of the organism, especially the reduction of the secretion amount and the reduction of the activity of digestive enzyme, so that the long-term nitrogen source imbalance of the organism is caused. It is clear what the body is short of protein for a long time, why old people lose muscle severely, and what the lack of protein is that you have protein sources in the body that are not digested, causing the body to absorb no protein nutrients, when the body cells use their own muscle proteins for survival. The protein-deficient organism is in a state of vicious circle, the elderly have no way to use the protein-deficient organism, the digestive function of the body is reduced, the protein in the body cannot be digested, and anyone cannot absorb the protein. Because macromolecular protein can not enter the body through the small intestine of the human body, and the protein is not digested and fermented in the intestinal tract to generate a plurality of toxins such as skatole and the like, the moisture is absorbed into the body through the large intestine to hurt the body.

The digestion-free protein product is the only method for solving the problem of insufficient protein caused by the fact that the aged cannot completely digest protein due to aging. Protein nutrition plays an important role in the aging process of human bodies, and nutrient imbalance not only accelerates organ aging, but also causes various diseases. Another manifestation of aging in the elderly is: the metabolism is slowed down, the digestion function is reduced, in particular, various hormones in the body are reduced, the hormones are produced by the human body, the basic substances of the hormones are all proteins, the hormones are important substances for regulating the normal activities of the body, the hormones, enzymes in the body and immune cells are all proteins, the proteins are the most important nutrients of the body, and the lack of the nutrients can directly influence the health of the body.

The indigestible protein is a protein with a molecular weight below 500 Da. Wherein the compound amino acid has an average molecular weight of 120Da and the small molecular dipeptide and tripeptide have molecular weights of 180-500 Da. Scientists have discovered during the study of genes that the human body has a substance that determines all functional activities of the human body's life, called peptides. It is pointed out that peptides are a revolutionary product that will lead the human nutritional revolution in the 21 st century. The human has been undergoing three health revolution since history, the first smallpox appeared, the solution was the vaccinium nikoense, the second penicillin was used to treat the disease caused by bacteria and virus, the third was the use of small molecule active peptide to treat all diseases including all major diseases, which was the problem of cell failure. Medical science states that only one disease of human is cell failure, which is caused by two causes, 1, the cells are not nourished. 2. The cytotoxin is overloaded. .

Disclosure of Invention

The new oyster protein food is developed, the content of the dry oyster protein is 45-57%, the dry oyster protein content is higher than that of soybean protein, the digestibility reaches 85%, 18 kinds of amino acids are complete, and the content of 8 kinds of essential amino acids is moderate. The oyster is the first cultured shellfish in the world, is one of important marine biological resources available to human beings, and has unique health care function and important value.

The invention aims to provide a digestion-free oyster protein food prepared from oysters. The oyster protein product can be directly absorbed through small intestine without digestion, and solves the problem of protein imbalance of the old, wherein the most important substance for human health is protein, and no protein exists, so that no life exists.

Provides a non-digestion protein product of micromolecule dipeptide and tripeptide prepared by taking oyster as a raw material in society, and has another expression of aging of the old: metabolism slows down, digestion function reduces, especially in vivo various hormones are reduced, hormone is produced by human body and is an important substance for regulating normal activities of the body, hormone and immune cells in the body are all protein, so protein nutrient is the most important nutrient of the human body, is absolutely indispensable, and is a substance coexisting with living organism, and the philosopher EngeL defines: life is a living form of a protein.

The preparation process comprises the following steps:

firstly, coarse crushing raw oysters by using water with the weight 1 to 7 to 8 times of that of the raw oysters, and then refining the raw oysters by using a colloid mill until oyster slurry passes through a 80-mesh screen;

secondly, sterilizing and modifying the refined oyster protein feed liquid, and instantaneously treating the oyster protein feed liquid at 125-130 ℃ for 10-15 seconds;

thirdly, the oyster protein liquid after sterilization and protein denaturation treatment is cooled to 54-56 ℃;

fourthly, adding 0.3 to 0.5 weight percent of compound protease into the cooled feed liquid, slowly stirring, controlling the temperature to be between 54 and 56 ℃, and carrying out deep enzymolysis for 36 to 72 hours to reach the standard requirement of products below 500 daltons, wherein the pH value does not need to be adjusted in the whole enzymolysis process;

fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished, and removing insoluble substances;

sterilizing and inactivating enzyme, keeping the temperature of the feed liquid at 90-95 deg.C for 15-25 s to kill protease;

seventhly, concentrating the solid material to be more than 40 percent by a double-effect evaporator, and carrying out spray drying to obtain a digestion-free oyster protein powder product;

and eighthly, mixing the sterilized and enzyme-deactivated feed liquid according to the standard of the liquid product, and performing aseptic filling to obtain the digestion-free oyster protein liquid product.

The invention has the following advantages and positive effects:

1. the content of the dry oyster protein is 45-57 percent, which is higher than that of the soybean protein, belongs to high-quality protein, and provides a powdery and liquid product of the oyster protein without digestion for the society.

2. Develops a brand-new oyster protein product without digestion, provides a small molecular protein product which can be directly absorbed without digestion for the health of the old or patients, and has great help for the health of the old or patients and the function and the value which cannot be achieved by the traditional product.

3. Provides a small molecule dipeptide and tripeptide protein product which is free from digestion in the market. The small molecular dipeptide and tripeptide are the components of human immune cells, and can proliferate the human immune cells and activate the human immune cells after entering the body. The function of immune cells in human bodies can kill variant and cancer cells in the human bodies, and the principle of the existing immunotherapy is that the immune cells are injected into the human bodies after being proliferated in vitro, so that the immune cells finish the task of killing the cancer cells, so that: proliferation of human immune cells is the most effective method for disease prevention and treatment of cancer.

There are only two ways for humans to obtain peptides:

one, eating small molecular dipeptide and tripeptide with molecular weight of 180 Da-500 Da.

Secondly, the peptide product is injected into the body through injection. Insulin, for example, is the 51 peptide, which saves countless lives of diabetics worldwide.

Detailed Description

Example 1:

weighing 500kg of oysters, carrying out coarse crushing treatment by using water with the weight 1: 7-8 times, and then refining by using a colloid mill until oyster slurry passes through a 80-mesh screen;

secondly, sterilizing and protein denaturing the refined oyster protein liquid, and instantaneously treating the oyster protein liquid at 120-130 ℃ for 10-15 seconds;

thirdly, quickly cooling the sterilized oyster protein liquid subjected to protein denaturation treatment to 54-56 ℃;

adding 2kg (0.3-0.5%) of compound protease into the cooled feed liquid, slowly stirring, controlling the temperature to be between 54 and 56 ℃, and carrying out deep enzymolysis for 36 to 72 hours or more until the product reaches the standard, wherein the pH value is not required to be adjusted in the whole enzymolysis process;

fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished, and removing insoluble substances;

sterilizing and inactivating enzyme, keeping the temperature of the feed liquid at 90-95 deg.C for 15-25 s to kill protease;

seventhly, concentrating the solid material to be more than 40 percent by a double-effect evaporator, and carrying out spray drying to obtain a digestion-free oyster protein powder product;

and eighthly, mixing the sterilized and enzyme-deactivated feed liquid according to the standard of the liquid product, and performing aseptic filling to obtain the digestion-free oyster protein liquid product.

Example 2:

weighing 1000kg of oysters, roughly crushing the oysters by using water with the weight 1 to 7 to 8 times of that of the oysters, and then refining the oysters by using a colloid mill until oyster slurry passes through a 80-mesh screen;

secondly, sterilizing and modifying the refined oyster liquid, and instantaneously treating the oyster liquid at 120-130 ℃ for 10-15 seconds;

thirdly, quickly cooling the sterilized oyster liquid subjected to protein denaturation treatment to 54-56 ℃;

adding 4kg (0.3-0.5%) of compound protease into the cooled feed liquid, slowly stirring, controlling the temperature to be between 54 and 56 ℃, and carrying out deep enzymolysis for 36 to 72 hours or more until the product reaches the standard, wherein the pH value is not required to be adjusted in the whole enzymolysis process;

fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished, and removing insoluble substances;

sterilizing and inactivating enzyme, keeping the temperature of the feed liquid at 90-95 deg.C for 15-25 s to kill protease;

seventhly, concentrating the solid material to be more than 40 percent by a double-effect evaporator, and carrying out spray drying to obtain an active digestion-free oyster protein powder product;

and eighthly, preparing the feed liquid after the sterilization and the enzyme deactivation according to the standard of the liquid product, and performing aseptic filling to obtain the active digestion-free oyster protein liquid product.

Claims (2)

1. A method for preparing digestion-free oyster protein by taking oysters as raw materials is characterized by comprising the following steps: the oyster is taken as a protein raw material, deep enzymolysis is carried out, and the small molecular protein which has the molecular weight of below 500 daltons and can be directly absorbed is achieved, wherein the small molecular protein comprises compound amino acid with the average molecular weight of 120 daltons, small molecular dipeptide with the molecular weight of 180-500 daltons and tripeptide;

the preparation process comprises the following steps:

coarse crushing raw oysters with water in an amount which is 1 to 7 to 8 times of the weight of the raw oysters, and refining the raw oysters by using a colloid mill until oyster protein slurry passes through a 80-mesh screen;

secondly, sterilizing and modifying the refined oyster protein liquid, and instantaneously treating the oyster protein liquid at 125-130 ℃ for 10-15 seconds;

thirdly, the oyster protein liquid after sterilization and protein denaturation treatment is cooled to 54-56 ℃;

fourthly, adding 0.3 to 0.5 percent of compound protease by weight of the raw materials into the cooled feed liquid, slowly stirring, controlling the temperature to be between 54 and 56 ℃, and carrying out deep enzymolysis for 36 to 72 hours to reach the standard requirement of products below 500 daltons, wherein the pH value does not need to be adjusted in the whole enzymolysis process;

fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished, and removing insoluble substances;

sterilizing and inactivating enzyme, keeping the temperature of the feed liquid at 90-95 deg.C for 15-25 s to reach the activity of sterilizing and inactivating protease;

seventhly, concentrating the solid material to be more than 40 percent by a double-effect evaporator, and carrying out spray drying to obtain a digestion-free oyster protein powder product;

and eighthly, mixing the sterilized and enzyme-deactivated feed liquid according to the standard of the liquid product, and performing aseptic filling to obtain the digestion-free oyster protein liquid product.

2. The method for preparing oyster protein without digestion according to claim 1, wherein the oyster protein is prepared from oyster as protein raw material.