CN113040263A - Preparation method for producing digestion-free protein by using sesame seed meal and soybean meal as raw materials - Google Patents
Preparation method for producing digestion-free protein by using sesame seed meal and soybean meal as raw materials Download PDFInfo
- Publication number
- CN113040263A CN113040263A CN202110441266.XA CN202110441266A CN113040263A CN 113040263 A CN113040263 A CN 113040263A CN 202110441266 A CN202110441266 A CN 202110441266A CN 113040263 A CN113040263 A CN 113040263A
- Authority
- CN
- China
- Prior art keywords
- protein
- meal
- sesame seed
- raw materials
- free protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 67
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 66
- 235000012054 meals Nutrition 0.000 title claims abstract description 35
- 235000003434 Sesamum indicum Nutrition 0.000 title claims abstract description 28
- 239000002994 raw material Substances 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 235000019764 Soybean Meal Nutrition 0.000 title claims description 13
- 239000004455 soybean meal Substances 0.000 title claims description 13
- 244000000231 Sesamum indicum Species 0.000 title 1
- 241000207961 Sesamum Species 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 239000000463 material Substances 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 5
- 108010016626 Dipeptides Proteins 0.000 claims abstract description 4
- 150000001413 amino acids Chemical class 0.000 claims abstract 2
- 239000002131 composite material Substances 0.000 claims abstract 2
- 239000000047 product Substances 0.000 claims description 15
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 239000012263 liquid product Substances 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 150000001875 compounds Chemical class 0.000 claims description 7
- 239000004615 ingredient Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 5
- 239000000084 colloidal system Substances 0.000 claims description 4
- 230000002779 inactivation Effects 0.000 claims description 4
- 238000007670 refining Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 238000001694 spray drying Methods 0.000 claims description 4
- 238000012371 Aseptic Filling Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 201000006549 dyspepsia Diseases 0.000 claims 3
- 230000000844 anti-bacterial effect Effects 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 244000046052 Phaseolus vulgaris Species 0.000 abstract description 12
- 235000010627 Phaseolus vulgaris Nutrition 0.000 abstract description 12
- 235000013305 food Nutrition 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000017060 Arachis glabrata Nutrition 0.000 abstract 3
- 244000105624 Arachis hypogaea Species 0.000 abstract 3
- 235000010777 Arachis hypogaea Nutrition 0.000 abstract 3
- 235000018262 Arachis monticola Nutrition 0.000 abstract 3
- 235000020232 peanut Nutrition 0.000 abstract 3
- 239000003205 fragrance Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 51
- 210000004027 cell Anatomy 0.000 description 28
- 230000032683 aging Effects 0.000 description 8
- 108090000765 processed proteins & peptides Proteins 0.000 description 8
- 210000002865 immune cell Anatomy 0.000 description 7
- 230000029087 digestion Effects 0.000 description 6
- 210000002784 stomach Anatomy 0.000 description 5
- 230000004900 autophagic degradation Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 3
- 208000008425 Protein deficiency Diseases 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 210000000813 small intestine Anatomy 0.000 description 3
- 102000003839 Human Proteins Human genes 0.000 description 2
- 108090000144 Human Proteins Proteins 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 210000004211 gastric acid Anatomy 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 239000011344 liquid material Substances 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 239000013055 pulp slurry Substances 0.000 description 2
- 238000004537 pulping Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Chemical compound C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000008934 Muscle Proteins Human genes 0.000 description 1
- 108010074084 Muscle Proteins Proteins 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- 241000700647 Variola virus Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000001640 apoptogenic effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- -1 compound amino acid Chemical class 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229940074386 skatole Drugs 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a preparation method for producing digestion-free protein by using sesame seed meal and bean pulp as raw materials, and relates to a production method of food, wherein sesame seed meal has a protein content of about 35 percent and has special fragrance, and peanut meal has a protein content of more than 45 percent, and two vegetal digestion-free protein liquid and powder products are obtained by using sesame and peanut meal as raw materials through deep enzymolysis. The indigestible protein is protein with molecular weight below 500Da, and is one kind of indigestible protein product with sesame and peanut protein as material and comprising composite amino acid with average molecular weight of 120Da and small molecular dipeptide and tripeptide with molecular weight of 180-500 Da.
Description
Technical Field
The invention relates to a preparation method of food, in particular to a preparation method for producing digestion-free protein by using sesame seed meal and bean meal as raw materials.
Background
According to the latest theories of life and physiology: the human body is in the growth period before the age of 25, in the growth and maturity period from the age of 25 to the age of 30, all body functions can begin to decline after the age of 30, and organs and functions are mostly reduced after the age of 60. The new idea is that the aging of the old people is not natural aging but the aging of the body can not completely recycle endogenous cell protein, and the body is aged in advance due to the long-term insufficient body protein. The physiology states that: the life cycle of human intestinal cells is 43 hours at most, the life cycle of cells on the surface of the stomach is three days, old cells are exfoliated as new cells grow out, the cells are digested and utilized by the human body like food, and normal people have 8-inch and two-inch exfoliated cells, namely 248.8 g of intestinal cell proteins are exfoliated every day. Half jin of human body cell protein with the biological potency of 100 is optimal, the exfoliated cell protein of the stomach is not considered, the cell protein of the small intestine is not denatured by gastric acid, the molecule winding is very tight and is not easy to digest, and one digestion by protease in the stomach is omitted, so the exfoliated cell protein of the normal young people needs to be digested for a long time at the lower end of the small intestine to be completely recycled.
The human protein supply consists of three parts:
the first part supplies: is cell protein dropped from intestinal tract, the dropped cell protein is digested and reused by human body like food, adult has at least 8 engbi and 31.1 g per engbi per day, namely 248.8 g of dropped cell protein, the cell protein is human protein, is the best quality protein with 100 biological value, and the biological value of any other kind of protein is lower than it.
A second part supplying: is dead cells in tissues, about 198-283 g of dead cells are repeatedly used by autophagy of human bodies every day, and autophagy means that the dead cells eat themselves. The existence of enzymes in living human cells that digest proteins, carbohydrates and fats can digest apoptotic cells for reuse by living cells is called autophagy. Looking up "one day hungry secret" autophagy science on the web, doctor university swedish japan scientist in 2016 thus won the nobel prize.
The third part supplies: is protein in food, and is supplemented by 1.0 g to 1.5 g per kilogram of body weight per day. The protein in food is digested in stomach, the protease secreted by human stomach is inactive and activated by gastric acid to digest the protein in food, and the aging of human body is the decrease of secretion in body which directly affects the digestion of protein in body.
The old people can not completely recycle endogenous cell protein due to the aging of various functions of the body, especially the reduction of the secretion amount and activity of digestive enzyme, so that the long-term protein deficiency of the body is caused. It is clear what the body's long-term protein deficiency is as a result, why old people lose muscle so badly that the body cells use their own muscle proteins for survival. The protein-deficient organism is in a state of vicious circle, the old has no way, the digestive function of the body is reduced, and the protein in the body is not digested, namely the protein is absorbed and utilized. And the protein is not digested and fermented in the intestinal tract to generate a plurality of toxins such as skatole and the like, and is absorbed by the large intestine to hurt the body, and the digestion-free protein product is the only method for solving the problem of protein deficiency of the old and has great significance for the health of the old.
The indigestible protein is a small molecule protein with a molecular weight below 500 Da. Wherein the compound amino acid has an average molecular weight of 120Da and the small molecular dipeptide and tripeptide have molecular weights of 180-500 Da. Scientists have discovered that the human body has a substance, called a peptide, that determines all functional activities of the human body's life when studying genes. He speaks: peptide is a revolutionary product, which will lead the nutritional revolution of human in the 21 st century, and human has been subjected to three health revolution in history, the first smallpox appeared, the solution was the seed of vaccinia, the second treatment of diseases caused by bacteria and viruses with penicillin, and the third treatment of all diseases including all major diseases with small active peptides. The medicine shows that: only one disease in humans is cytopathy. There are two causes of cytopathic effects: 1. the cells are not nourished. (not getting the needed things) 2, cytotoxic overload.
(unwanted material is obtained).
The invention aims to solve the problem that the old people have insufficient protein in vivo due to the insufficient recovery and utilization of cell protein in vivo caused by the aging of the body, realize the in vitro digestion by using a biological enzyme engineering technology, provide a protein product which can be directly absorbed through the small intestine without digestion, and solve the problem of protein imbalance of the old people.
Disclosure of Invention
The invention aims to provide a preparation method for producing digestion-free protein by using sesame seed meal and soybean meal as raw materials, which provides a protein product digested in vitro for the old, can be directly absorbed, and solves the problem of pain of insufficient protein nutrients caused by incomplete digestion of protein due to aging of the old. The process route is simple, and the sesame meal and the bean meal which are eaten after oil immersion are utilized to directly degrade the protein through a biological enzyme engineering technology so as to achieve the purpose of directly absorbing the small molecular protein.
The process steps of the invention are as follows:
firstly, mixing the sesame seed meal and the soybean meal raw materials with water in a ratio of 1 to 8-10 times;
secondly, refining the sesame seed meal and the soybean meal feed liquid after size mixing by using a colloid mill, wherein the fineness is more than 100 meshes;
heating to sterilize and denaturalize protein, and keeping the temperature at least 135 ℃ for more than 30 seconds;
fourthly, the temperature of the heated feed liquid is adjusted to 54 ℃ to 56 ℃, 0.3 percent to 0.5 percent of compound protease is added for deep enzymolysis, the mixture is stirred at a low speed, the temperature is controlled between 54 ℃ and 56 ℃ all the time, the enzymolysis time is 36 hours to 72 hours, and the pH value does not need to be adjusted in the whole enzymolysis process;
fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished to remove insoluble substances;
fifthly, the fifth step is respectively proportioned according to the standard of powder and liquid products, and then sterilization and enzyme inactivation treatment are carried out, the temperature is required to reach more than 90 ℃, and the temperature is kept for 15 to 25 seconds, so as to achieve the purpose of sterilization and enzyme inactivation;
seventhly, concentrating the sterilized product ingredients by a double-effect evaporator to more than 40 percent, and performing spray drying to obtain a digestion-free protein powder product taking the sesame seed meal and the bean meal as raw materials;
and (viii) mixing the sterilized and enzyme-deactivated liquid products, and aseptically filling to obtain the non-digestible protein liquid product using sesame seed meal and bean meal as raw materials.
The invention has the following advantages and positive effects:
1. in order to solve the problem of insufficient protein of the old people due to the aging of the body and the insufficient recovery and utilization of the cell protein in the body, the high-quality protein of the sesame seed meal and the bean meal which can only be used as feed protein sources in the prior art is purified by utilizing a biological enzyme engineering technology to produce two vegetal digestion-free protein products which can be directly absorbed.
2. The utilization value of the sesame seed meal and the soybean meal is improved, the sesame seed meal and the soybean meal can only be used as animal feed before, animal protein which can be utilized by human bodies is generated, and the digestion-free protein product which can be directly utilized by the human bodies is purified and processed by biotechnology at present.
3. Provides a small molecule peptide product which is really free from digestion in the market. The small molecular peptide is a component of human immune cells, and can proliferate the human immune cells and activate the human immune cells after entering a body. American shake video: the whole process of killing cancer cells by immune cells in a human body, and the principle of the existing immunotherapy is that the immune cells are injected into the human body after being proliferated in vitro, so that the immune cells complete the task of killing the cancer cells, so that: proliferating human immune cells is the most effective method for preventing and treating cancer.
The peptide obtained by human body has only two ways, namely, the small molecular dipeptide and tripeptide with the edible molecular weight of 180 Da-500 Da. Secondly, the peptide product is injected into the body through injection. For example, insulin is the 51 peptide, which must be injected, cannot be taken orally, and saves countless lives of diabetics worldwide.
Detailed Description
Example 1:
1. weighing 100kg of raw materials of sesame seed meal and bean pulp respectively, mixing and pulping by using 9 times of water, namely adding 1800kg of production water;
2. refining the adjusted sesame seed meal and bean pulp slurry by a colloid mill, wherein the fineness is more than 100 meshes;
3. carrying out ultra-high temperature sterilization and protein denaturation treatment on the refined feed liquid, and preserving the heat for more than 30 seconds at the temperature of at least 135 ℃;
4. cooling the material liquid to 54-56 deg.c;
5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, adding 0.8 kg of compound protease in the example, wherein the enzymolysis time is 70-75 hours, and the pH value is not required to be adjusted in the enzymolysis process, but the slow stirring treatment is required;
6. carrying out centrifugal separation treatment on the enzymolysis feed liquid to remove insoluble substances, mixing the materials according to the standard of powdery and liquid products, and then carrying out enzyme deactivation treatment on the feed liquid for 30 seconds at 90 ℃ to obtain a digestion-free protein liquid raw material taking sesame meal and bean meal as raw materials;
7. carrying out aseptic filling on the sterilized and enzyme-inactivated liquid material of the liquid ingredient to obtain a liquid product;
8. and concentrating the feed liquid of the sterilized and enzyme-deactivated powdery ingredients by a double-effect evaporator to a solid content of more than 40%, and performing spray drying to obtain a powdery product.
Example 2:
1. weighing the raw materials of sesame seed meal and bean pulp respectively by 200kg, mixing and pulping by using 9 times of water, namely adding 3600kg of production water;
2. refining the adjusted sesame seed meal and bean pulp slurry by a colloid mill, wherein the fineness is more than 100 meshes;
3. carrying out ultra-high temperature sterilization and protein denaturation treatment on the refined feed liquid, and preserving the heat for more than 30 seconds at the temperature of at least 135 ℃;
4. cooling the material liquid to 54-56 deg.c;
5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, wherein 1.6 kg of compound protease is added in the example, the enzymolysis time is 70-75 hours, and the pH value is not required to be adjusted in the enzymolysis process, but the slow stirring treatment is required;
6. carrying out centrifugal separation treatment on the enzymolysis feed liquid to remove insoluble substances, mixing the materials according to the standard of powdery and liquid products, and then carrying out enzyme deactivation treatment on the feed liquid for 30 seconds at 90 ℃ to obtain a digestion-free protein liquid raw material taking sesame meal and bean meal as raw materials;
7. carrying out aseptic filling on the sterilized and enzyme-inactivated liquid material of the liquid ingredient to obtain a liquid product;
8. and concentrating the feed liquid of the sterilized and enzyme-deactivated powdery ingredients by a double-effect evaporator to a solid content of more than 40%, and performing spray drying to obtain a powdery product.
Claims (2)
1. A preparation method for producing digestion-free protein by using sesame seed meal and soybean meal as raw materials is characterized by comprising the following steps: the ratio of the sesame seed meal to the soybean meal raw material is 1: 2-2: 1.
The process steps of the invention are as follows:
firstly, mixing the sesame seed meal and the soybean meal raw materials with water in a ratio of 1 to 8-10 times;
secondly, refining the sesame seed meal and the soybean meal feed liquid after size mixing by using a colloid mill, wherein the fineness is more than 100 meshes;
heating to sterilize and denaturalize protein, and keeping the temperature at least 135 ℃ for more than 30 seconds;
fourthly, the temperature of the heated feed liquid is adjusted to 54 ℃ to 56 ℃, 0.3 percent to 0.5 percent of compound protease is added for deep enzymolysis, the mixture is stirred at a low speed, the temperature is controlled between 54 ℃ and 56 ℃ all the time, the enzymolysis time is 36 hours to 72 hours, and the pH value does not need to be adjusted in the whole enzymolysis process;
fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished to remove insoluble substances;
fifthly, the fifth step is respectively proportioned according to the standard of powder and liquid products, and then sterilization and enzyme inactivation treatment are carried out, the temperature is required to reach more than 90 ℃, and the temperature is kept for 15 to 25 seconds, so as to achieve the purpose of sterilization and enzyme inactivation;
seventhly, concentrating the material to be more than 40 percent by the powdery bactericidal and enzyme-inactivated product ingredient through a double-effect evaporator, and performing spray drying to obtain a digestion-free protein powdery product;
and eighthly, mixing the sterilized and enzyme-inactivated liquid product, and performing aseptic filling to obtain the digestion-free protein liquid product.
2. The method for preparing the indigestion-free protein by using the sesame seed meal and the soybean meal as raw materials according to claim 1, which is characterized in that edible sesame seed meal and soybean meal after oil immersion are used as raw materials, and liquid and powder products of the indigestion-free protein are produced by compound enzyme degradation, wherein the indigestion-free protein is small molecular protein with the molecular weight below 500Da (Dalton). I.e. small molecular dipeptide and tripeptide with molecular weight between 180Da and 500Da and composite amino acid with molecular weight of 120 Da.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110441266.XA CN113040263A (en) | 2021-04-23 | 2021-04-23 | Preparation method for producing digestion-free protein by using sesame seed meal and soybean meal as raw materials |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110441266.XA CN113040263A (en) | 2021-04-23 | 2021-04-23 | Preparation method for producing digestion-free protein by using sesame seed meal and soybean meal as raw materials |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113040263A true CN113040263A (en) | 2021-06-29 |
Family
ID=76520055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110441266.XA Pending CN113040263A (en) | 2021-04-23 | 2021-04-23 | Preparation method for producing digestion-free protein by using sesame seed meal and soybean meal as raw materials |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113040263A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100897A (en) * | 2019-04-22 | 2019-08-09 | 长春大学 | A kind of preparation method of small-molecular peptides milk and small-molecular peptides milk powder |
| CN110564799A (en) * | 2019-09-19 | 2019-12-13 | 长春大学 | Preparation method for producing enzyme activity digestion-free protein by using soybean protein isolate as raw material |
| CN110637918A (en) * | 2019-11-07 | 2020-01-03 | 长春大学 | Preparation method for producing digestion-free protein by using soybean protein isolate as raw material |
| CN111345344A (en) * | 2019-12-26 | 2020-06-30 | 长春大学 | A method for preparing liquid and powder product of digestion-free milk (containing goat milk) |
-
2021
- 2021-04-23 CN CN202110441266.XA patent/CN113040263A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100897A (en) * | 2019-04-22 | 2019-08-09 | 长春大学 | A kind of preparation method of small-molecular peptides milk and small-molecular peptides milk powder |
| CN110564799A (en) * | 2019-09-19 | 2019-12-13 | 长春大学 | Preparation method for producing enzyme activity digestion-free protein by using soybean protein isolate as raw material |
| CN110637918A (en) * | 2019-11-07 | 2020-01-03 | 长春大学 | Preparation method for producing digestion-free protein by using soybean protein isolate as raw material |
| CN111345344A (en) * | 2019-12-26 | 2020-06-30 | 长春大学 | A method for preparing liquid and powder product of digestion-free milk (containing goat milk) |
Non-Patent Citations (1)
| Title |
|---|
| 王振斌，等: "芝麻饼粕蛋白质的理化性质和功能性质研究", 《中国粮油学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110637918A (en) | Preparation method for producing digestion-free protein by using soybean protein isolate as raw material | |
| CN110564799A (en) | Preparation method for producing enzyme activity digestion-free protein by using soybean protein isolate as raw material | |
| CN111387336A (en) | Preparation method of high branched chain amino acid digestion-free corn protein product | |
| CN111345344A (en) | A method for preparing liquid and powder product of digestion-free milk (containing goat milk) | |
| CN111019992A (en) | Preparation method for producing digestion-free protein by using animal protein as raw material | |
| CN109875052A (en) | A kind of three clean bodies prebiotics exempt from the preparation method of digestion mung bean protein peptide | |
| CN101692908A (en) | Double-glue pollen food with functions of enriching blood, beautifying face and nourishing skin and preparation method thereof | |
| CN110100897A (en) | A kind of preparation method of small-molecular peptides milk and small-molecular peptides milk powder | |
| CN111004829A (en) | Preparation method of black fungus active peptide | |
| CN112890064A (en) | Composite collagen peptide solid beverage and preparation method thereof | |
| JP4132635B2 (en) | Uninactivated enzyme-enhanced composition | |
| CN109601695A (en) | A kind of method that activated protein exempts from digesting protein with soybean protein for raw material preparation | |
| CN103876147A (en) | Method for producing enzyme by using black soya bean tempeh | |
| CN1531399A (en) | Biologically active food additive and biologically active forage additive for preventing iodine deficiency and optimising iodine metabolism, food and forage products containing said additive | |
| CN112825958A (en) | Method for preparing digestion-free double protein by using live beads and soybean protein as raw materials | |
| CN102379391A (en) | Preparation of health food of pine pollen, glucan and natto | |
| CN113040263A (en) | Preparation method for producing digestion-free protein by using sesame seed meal and soybean meal as raw materials | |
| CN107278721A (en) | The method for preventing and treating " Red Male " actinidia tree canker | |
| CN111616371A (en) | Preparation method for producing digestion-free protein by using various egg white proteins as raw materials | |
| CN113040261A (en) | Preparation method for producing digestion-free protein by using sesame meal and peanut meal as raw materials | |
| CN113040262A (en) | Preparation method for producing digestion-free sesame protein by using sesame seed meal as raw material | |
| CN113040260A (en) | Preparation method for producing digestion-free protein by using sesame seed meal and corn germ meal as raw materials | |
| CN113040259A (en) | Method for preparing digestion-free protein by using four meal of sesame, soybean, peanut and corn germ as raw materials | |
| CN113854398A (en) | Method for preparing digestion-free walnut protein by using walnut cake meal as raw material | |
| CN113841783A (en) | Method for preparing digestion-free oyster protein by taking oysters as raw materials |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210629 |
|
| WD01 | Invention patent application deemed withdrawn after publication |