CN113040261A - Preparation method for producing digestion-free protein by using sesame meal and peanut meal as raw materials - Google Patents
Preparation method for producing digestion-free protein by using sesame meal and peanut meal as raw materials Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a preparation method for producing a digestion-free protein by using sesame seed meal and peanut meal as raw materials, and relates to a production method of a food, wherein the sesame seed meal has a protein content of about 35 percent and has special fragrance, the peanut meal has a protein content of more than 45 percent, and two plant digestion-free protein liquid and powder products are obtained by using sesame and peanut meal as raw materials through deep enzymolysis. The indigestible protein is protein with molecular weight below 500Da, and is one kind of indigestible protein product with sesame and peanut protein as material and comprising composite amino acid with average molecular weight of 120Da and small molecular dipeptide and tripeptide with molecular weight of 180-500 Da.
Description
Technical Field
The invention relates to a preparation method of food, in particular to a preparation method for producing digestion-free protein by using sesame meal and peanut meal as raw materials.
Background
According to the life science and physiology, the method comprises the following steps: the human body is in the growth period before the age of 25, in the growth and maturity period from the age of 25 to the age of 30, the body functions begin to decline after the age of 30, and the organs and functions decline greatly after the age of 60.
The new view point is that,
At present, the aging of old people rarely has natural aging, but endogenous cellular protein can not be completely recycled after the aging of organisms, and the long-term shortage of the protein of the organisms is the reason of the premature aging of the organisms. The physiology states that: the life cycle of human intestinal cells is 43 hours at most, the life cycle of cells on the surface of the stomach is three days, old cells are exfoliated as new cells grow out, the cells are digested and reused by the human body like food, and normal people have 8-inch and two-inch exfoliated cells, namely 248.8 g of intestinal cell proteins are exfoliated every day. Half a kilogram of human cellular proteins with a biological titer of 100 are best quality, which does not take into account the amount of shed cellular proteins from the stomach. The cell protein of the small intestine is not denatured by gastric acid, the molecule winding is very tight and is difficult to contact with the digestive enzyme for digestion, and meanwhile, the digestion of the protease in the stomach is reduced by one time, so the cell protein of the normal young people which is partially removed needs to be digested for a long time at the lower end of the small intestine to be completely recycled.
The human protein supply consists of three parts:
the first part supplies: is cell protein dropped from intestinal tract, the dropped cell protein is digested and reused by human body like food, adult has at least 8 engbi and 31.1 g per engbi per day, namely 248.8 g of dropped cell protein, the cell protein is human protein, is the best quality protein with 100 biological value, and the biological value of any other kind of protein is lower than it.
A second part supplying: is dead cells in tissues, about 198-283 g of dead cells are repeatedly used by autophagy of human bodies every day, and autophagy means that the dead cells eat themselves. The existence of enzymes in living human cells that digest proteins, carbohydrates and fats can digest apoptotic cells for reuse by living cells is called autophagy. Looking up "one day hungry secret" autophagy science on the web, doctor university swedish japan scientist in 2016 thus won the nobel prize.
The third part supplies: is protein in food, and is supplemented by 1.0 g to 1.5 g per kilogram of body weight per day. The protein in food is digested in stomach, the protease secreted by human stomach is inactive and activated by gastric acid to digest the protein in food, and the aging of human body is the decrease of secretion in body which directly affects the digestion of protein in body.
The old people can not completely recycle endogenous cell protein due to the aging of various functions of the body, especially the reduction of the secretion amount and activity of digestive enzyme, so that the long-term protein deficiency of the body is caused. It is clear what the body's long-term protein deficiency is as a result, why old people lose muscle so badly that the body cells use their own muscle proteins for survival. The protein-deficient organism is in a state of vicious circle, the old has no way, the digestive function of the body is reduced, the protein in the body can not be digested, and the macromolecular protein can not be absorbed and utilized by the human body. And the protein is not digested and fermented in the intestinal tract to generate a plurality of toxins such as skatole and the like, and is absorbed by the large intestine to hurt the body, and the digestion-free protein product is the only method for solving the problem of protein deficiency of the old and has great significance for the health of the old.
The indigestible protein is a small molecule protein with a molecular weight below 500 Da. Wherein the compound amino acid has an average molecular weight of 120Da and the small molecular dipeptide and tripeptide have molecular weights of 180-500 Da. Scientists have discovered that the human body has a substance, called a peptide, that determines all functional activities of the human body's life when studying genes. He speaks: peptide is a revolutionary product, which will lead the nutritional revolution of human in the 21 st century, and human has been subjected to three health revolution in history, the first smallpox appeared, the solution was the seed of vaccinia, the second treatment of diseases caused by bacteria and viruses with penicillin, and the third treatment of all diseases including all major diseases with small active peptides. The medicine shows that: only one disease in humans is cytopathy. There are two causes of cytopathic effects: 1. the cells are not nourished. (not getting the needed things) 2, cytotoxic overload.
(unwanted material is obtained).
The invention aims to solve the problem that the old people have insufficient protein due to insufficient recycling of in vivo cell protein caused by aging of the body, complete the digestion of the protein in vitro by using a biological enzyme engineering technology, provide a protein product which can be directly absorbed through the small intestine without digestion, and solve the problem of protein imbalance of the old people.
Disclosure of Invention
The invention aims to provide a preparation method for producing digestion-free protein by using sesame meal and peanut meal as raw materials, which provides protein food digested in vitro for the old, can be directly absorbed, and solves the problem of pain point of protein nutrient imbalance caused by incomplete digestion of protein due to aging of the old. The process route is simple, edible sesame meal and peanut meal after oil immersion are used as raw materials, and protein is directly degraded through a biological enzyme engineering technology, so that a small molecular protein product which is directly absorbed is achieved.
The process steps of the invention are as follows:
firstly, mixing the sesame seed meal and the peanut meal raw materials with water in a ratio of 1 to 8-10 times;
secondly, refining the sesame seed meal and the peanut meal feed liquid after size mixing by using a colloid mill, wherein the fineness is more than 100 meshes;
heating to sterilize and denaturalize protein, and keeping the temperature at least 135 ℃ for more than 30 seconds;
fourthly, the temperature of the heated feed liquid is adjusted to 54 ℃ to 56 ℃, 0.3 percent to 0.5 percent of compound protease is added for deep enzymolysis, the mixture is stirred at a low speed, the temperature is controlled between 54 ℃ and 56 ℃ all the time, the enzymolysis time is 36 hours to 72 hours, and the pH value does not need to be adjusted in the whole enzymolysis process;
fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished to remove insoluble substances;
fifthly, the fifth step is respectively proportioned according to the standard of powder and liquid products, and then sterilization and enzyme inactivation treatment are carried out, the temperature is required to reach more than 90 ℃, and the temperature is kept for 15 to 25 seconds, so as to achieve the purpose of sterilization and enzyme inactivation;
seventhly, concentrating the sterilized product ingredients of the powder product by a double-effect evaporator to obtain more than 40 percent of solid matters, and performing spray drying to obtain a digestion-free protein powder product taking sesame seed meal and peanut protein as raw materials;
and (viii) mixing the sterilized and enzyme-inactivated liquid product, and aseptically filling to obtain the digestion-free protein liquid product using sesame and peanut protein as raw materials.
The invention has the following advantages and positive effects:
1. the problem of the old people that the recovery and the utilization of the protein of cells in the body are insufficient due to the aging of the body, so that the protein of the body is insufficient for a long time is solved, the high-quality protein of the sesame seed meal and the peanut meal which can only be used as feed protein sources in the prior art is purified by utilizing a biological enzyme engineering technology, and two kinds of directly absorbable plant digestion-free protein products are produced.
2. The utilization value of the sesame seed meal and the peanut meal is improved, the sesame seed meal and the peanut meal can only be used as animal feed before, animal protein which can be utilized by human bodies is generated, and the digestion-free protein product which can be directly utilized by the human bodies is purified and processed by biotechnology at present.
3. Provides a small molecule peptide product which is really free from digestion in the market. The small molecular peptide is a component of human immune cells, and can proliferate the human immune cells and activate the human immune cells after entering a body. American shake video: the whole process of killing cancer cells by immune cells in a human body, and the principle of the existing immunotherapy is that the immune cells are injected into the human body after being proliferated in vitro, so that the immune cells complete the task of killing the cancer cells, so that: proliferating human immune cells is the most effective method for preventing and treating cancer.
The peptide obtained by human body has only two ways, namely, the small molecular dipeptide and tripeptide with the edible molecular weight of 180 Da-500 Da. Secondly, the peptide product is injected into the body through injection. For example, insulin is the 51 peptide, which must be injected, cannot be taken orally, and saves countless lives of diabetics worldwide.
Detailed Description
Example 1:
1. weighing 100kg of raw materials of sesame meal and peanut meal respectively, mixing and pulping by using 9 times of water, and adding 1800kg of production water;
2. refining the mixed sesame seed meal and peanut meal slurry by using a colloid mill, wherein the fineness is more than 100 meshes;
3. carrying out ultra-high temperature sterilization and protein denaturation treatment on the refined feed liquid, and preserving the heat for more than 30 seconds at the temperature of at least 135 ℃;
4. cooling the material liquid to 54-56 deg.c;
5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, adding 0.8 kg of compound protease in the example, wherein the enzymolysis time is 70-75 hours, and the pH value is not required to be adjusted in the enzymolysis process, but the slow stirring treatment is required;
6. carrying out centrifugal separation treatment on the enzymolysis feed liquid to remove insoluble substances, mixing the materials according to the standard of powdery and liquid products, and then carrying out enzyme deactivation treatment on the feed liquid for 30 seconds at 90 ℃ to obtain a digestion-free protein liquid raw material taking sesame meal and peanut meal as raw materials;
7. carrying out aseptic filling on the sterilized and enzyme-deactivated liquid feed liquid to obtain a liquid product;
8. and concentrating the sterilized and enzyme-deactivated powdery material liquid by a double-effect evaporator to a solid content of more than 40%, and performing spray drying to obtain a powdery product.
Example 2:
1. weighing the raw materials of sesame meal and peanut meal respectively by 200kg, mixing and pulping by using 9 times of water, and adding 3600kg of production water;
2. refining the mixed sesame seed meal and peanut meal slurry by using a colloid mill, wherein the fineness is more than 100 meshes;
3. carrying out ultra-high temperature sterilization and protein denaturation treatment on the refined feed liquid, and preserving the heat for more than 30 seconds at the temperature of at least 135 ℃;
4. cooling the material liquid to 54-56 deg.c;
5. adding 0.3-0.5% of compound protease by weight of the raw materials for enzymolysis, wherein 1.6 kg of compound protease is added in the example, the enzymolysis time is 70-75 hours, and the pH value is not required to be adjusted in the enzymolysis process, but the slow stirring treatment is required;
6. carrying out centrifugal separation treatment on the enzymolysis feed liquid to remove insoluble substances, mixing the materials according to the standard of powdery and liquid products, and then carrying out enzyme deactivation treatment on the feed liquid for 30 seconds at 90 ℃ to obtain a digestion-free protein liquid raw material taking sesame meal and peanut meal as raw materials;
7. carrying out aseptic filling on the sterilized and enzyme-deactivated liquid feed liquid to obtain a liquid product;
8. and concentrating the sterilized and enzyme-deactivated powdery material liquid by a double-effect evaporator to a solid content of more than 40%, and performing spray drying to obtain a powdery product.
Claims (2)
1. A preparation method for producing digestion-free protein by using sesame meal and peanut meal as raw materials is characterized by comprising the following steps: the ratio of the sesame seed meal to the peanut meal is 1: 2-2: 1.
The process steps of the invention are as follows:
weighing sesame seed meal and peanut meal as raw materials, and then mixing the raw materials with water in a ratio of 1: 8-10 times;
secondly, refining the sesame seed meal and the peanut meal feed liquid after size mixing by using a colloid mill, wherein the fineness is more than 100 meshes;
heating to sterilize and denaturalize protein, and keeping the temperature at least 135 ℃ for more than 30 seconds;
fourthly, the temperature of the heated feed liquid is adjusted to 54 ℃ to 56 ℃, 0.3 percent to 0.5 percent of compound protease is added for deep enzymolysis, the mixture is stirred at a low speed, the temperature is controlled between 54 ℃ and 56 ℃ all the time, the enzymolysis time is 36 hours to 72 hours, and the pH value does not need to be adjusted in the whole enzymolysis process;
fifthly, carrying out centrifugal separation on the feed liquid after the enzymolysis is finished to remove insoluble substances;
fifthly, the fifth step is respectively proportioned according to the standard of powder and liquid products, and then sterilization and enzyme inactivation treatment are carried out, the temperature is required to reach more than 90 ℃, and the temperature is kept for 15 to 25 seconds, so as to achieve the purpose of sterilization and enzyme inactivation;
seventhly, concentrating the material to be more than 40% by the powdery bactericidal and enzyme-inactivated product ingredient through a double-effect evaporator, and performing spray drying to obtain a digestion-free protein powdery product taking sesame seed meal and peanut meal as raw materials;
and (viii) mixing the sterilized and enzyme-inactivated liquid product, and aseptically filling to obtain the digestion-free protein liquid product with the sesame seed meal and the peanut meal as raw materials.
2. The method for preparing the indigestion-free protein by using the sesame seed meal and the peanut meal as raw materials according to claim 1, which is characterized in that edible sesame seed meal and edible peanut meal after oil immersion are used as raw materials, and liquid and powder products of the indigestion-free protein are produced by compound enzyme degradation, wherein the indigestion-free protein is small molecular protein with the molecular weight below 500Da (Dalton). I.e. small molecular dipeptide and tripeptide with molecular weight between 180Da and 500Da and composite amino acid with molecular weight of 120 Da.
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