CN115428855A

CN115428855A - Dipeptide tripeptide and amino acid product produced by vegetable protein and preparation method thereof

Info

Publication number: CN115428855A
Application number: CN202211085988.7A
Authority: CN
Inventors: 朱振伟; 朱光; 杨培珍; 朱莹
Original assignee: Jilin Province Wuliangshou Biotechnology Co ltd
Current assignee: Jilin Province Wuliangshou Biotechnology Co ltd
Priority date: 2022-09-06
Filing date: 2022-09-06
Publication date: 2022-12-06

Abstract

The invention discloses a dipeptide tripeptide and amino acid product produced by vegetable protein and a preparation method thereof, belonging to the technical field of biological enzyme. The method is characterized in that soybean protein, corn protein, mung bean protein and peanut protein are respectively used as raw materials, an acid and enzyme double-hydrolysis process is adopted, namely, the digestion process of a human body to the protein is simulated, so that a small molecular protein product with the molecular weight below 500Da is produced, and the science proves that only the part of protein can be directly absorbed and utilized by the human body. The purpose of the invention is as follows: the method is a unique method for solving the pain point that the aged cannot completely digest cell protein dropped in intestines due to the digestive function decline caused by aging to cause long-term protein deficiency, immune cells of the aged can be directly reduced due to the long-term protein deficiency, the disease and cancer incidence rate is increased, and a protein product which does not need to be digested is used. The medicine shows that: all disease occurs because cells do not receive the required nutrients and are overloaded with cytotoxins. The new product provides a mung bean protein product with protein directly absorbed without digestion and detoxification function.

Description

Dipeptide tripeptide and amino acid product produced by vegetable protein and preparation method thereof

The invention belongs to the technical field of biological enzyme engineering, and particularly relates to a plant small molecule protein product which is directly absorbed without digestion and is produced by producing dipeptide tripeptide and amino acid products from plant protein and a preparation method thereof.

The latest life science of the background art reveals for the first time that the aged cannot fully recycle endogenous cellular proteins dropped from the intestines of the body after aging, causing the healthy pain point of long-term insufficient proteins, from the aspects of preventive medicine, namely nutrition materials science, apoptosis and intestinal physiology. The invention provides a cholesterol-free vegetable protein product which can be directly absorbed without digestion in the market.

The life science library, the 'food and nutrition' book introduces: the average life of human small intestine cells is 43 hours, and the small intestine of an adult sheds a minimum of 8 engths of cellular protein every day, wherein the total weight of the engths is 31.1 g and is 248.8 g. The life of the intragastric cellular proteins is three days, and these apocrine cells are all the best human proteins with a biological titer of 100. These cellular proteins are digested and recovered by the human body in the small intestine like food. Dead cells in body tissues are autophagy by living cells and are eaten by the living cells to reach balance, and the autophagy is discovered for the first time by doctor of diad of giant Yu in Japan, so that the Nobel prize is obtained. Because the small intestine is arranged at the lower end of the stomach, the dropped cell protein is less than the protein in food by one gastric acid treatment and is digested in the stomach, untreated molecular groups are wound together, and protease is difficult to contact the surface of macromolecular protein, so the cell protein dropped from the small intestine can be digested and finished at the lower end of the small field for a long time and is recycled by a human body. The invention provides a method for solving the problem that the old people after aging cannot completely recycle cell proteins dropped in intestines, various toxins such as skatole and the like are generated by fermentation in the intestines, and the moisture of the intestines is absorbed into the body to damage body tissues and cells and cause long-term protein deficiency.

The body's immune cells are reduced first when there is insufficient protein in the body because the basic material of immune cells is protein. One of the three functions of the immune cell is to kill bacteria and viruses entering the body and to eliminate the mutated cancer cells in the body. The high incidence of cancer and disease in the elderly is directly related to protein deficiency.

Scientists have studied genes and discovered that the human body has a substance, called a peptide, which determines all functions and activities of the human body's life. The medical elaboration and the peptide can cure all diseases of all major diseases, namely the cytopathic problem. It is pointed out that only one disease in humans is cell failure. Cell failure is due to 2 causes, 1, the cells are not nourished. 2. The cytotoxin is overloaded.

From a nutritional point of view, people have 45 essential nutrients (oxygen and water) supplied in sufficient quantity in the life at present, and the guarantee of homeostasis after absorption is the most important substance guarantee for health. The problem of the elderly is that proteins are supplied and there is a source of cell-shedding proteins in the body, aging causes the digestive function to be deteriorated, the proteases thereof to be decreased and the activity thereof to be lowered, resulting in failure to completely digest the proteins in the body, and large proteins are not absorbed by anyone, resulting in a long-term shortage of proteins. The most important substance in human body nutrients is protein, which is the material basis for living organisms, the protein is the basic cell composing human body, repairing tissue and cell regeneration, the raw material for manufacturing enzymes and body fluid is the important substance for maintaining the acid-base balance and water-salt metabolism of life, and the human body is regenerated and repaired every time, thus ensuring the normal state of the organism.

Amino acids are the basic units that make up proteins and are also one of the important substances that ensure normal metabolism in the human body. There are 18 kinds of amino acids that human body can digest and absorb. Of these 9 amino acids are essential amino acids that are not synthesized in infants and adults and must be supplemented from the diet. While scientists thought that only amino acids could be absorbed and utilized by the body through the small intestine, with the development of science and technology, scientists found that small molecule dipeptide tripeptides could also be directly absorbed and utilized by the small intestine of human body and exert the physiological functions and actions of various small peptides in the body. Two scientists Newey and Smith in 1960 proved that glycine dipeptide can be completely absorbed by transport through experiments, and firstly proposed the convincing theory that dipeptide and tripeptide can be completely absorbed, and the idea that peptide can be directly absorbed and utilized by animals is gradually accepted. Beijing university department of medicine, liyong professor video lecture: studies are now conducted to demonstrate that dipeptides and tripeptides can be directly absorbed by the human body.

The existing amino acid products are generally hydrolyzed by acid, 6mol/L hydrochloric acid is usually adopted, and the hydrolysis needs high temperature, is carried out for 24 hours at 110 ℃ in a vacuum state, and is also carried out for 90 minutes at 150 ℃. The disadvantage is that tryptophan is completely destroyed and the serine and tyrosine are partially destroyed. Tryptophan is essential amino acid, the body cannot utilize protein to synthesize new cells and tissues due to the fact that protein in the body is lack of any essential amino acid, amino acid milk powder on the market is produced by an acid method, and tryptophan is completely destroyed, which is why infant amino acid milk powder sold on the market is not good for children after being used by large families.

The alkaline hydrolysis method and tryptophan are not destroyed, but serine, threonine, cysteine and arginine are destroyed to different degrees, and other amino acids produce L-type and D-type mixture. In the human life on earth, only L-type amino acid exists, and L-D mixture and D-type amino acid cannot be utilized, and the D-type amino acid can be utilized by livestock animals. The alkaline tryptophan is stable, and during hydrolysis, the arginine is deaminated, and the cysteine contains sulfur, so that the alkaline tryptophan has a very bad smell, and racemization is generated to produce a directly absorbed protein product.

The innovative process of the patent is as follows: the invented process simulates the digestion process of human body to protein, and the pH value of gastric acid in human body is about 0.9-1.5. The Ph of human intragastric diet is 1.8-3.5 digestion of diet from 2 hours to 6 hours, and varies depending on the form of the diet and the amount of fat in the protein, with more fat being digested for longer periods of time. The protease hydrolysis has the advantages of mildness and no damage to amino acids, but the enzyme hydrolysis is often incomplete, and the product has more peptides except the amino acids.

Telling a story: the wonderful cure is said to suggest that the early treatment of scurvy is suggested from one trip of Columbus to the new continent. At the time, the boat has several crevicular hands which are endangered, and the corpses are thrown into the sea, and are reluctant to die on the boat, and are left on a small island which they see. When the sailors die on the island, eat some wild fruits and plants, unexpectedly, the disease of the sailors is cured, and after a few months, the Columbus fleet returns to Europe. This island, which once healed the disease, is called the healing island. It is now known that the factor against scurvy is ascorbic acid, i.e. vitamin C. To state what, a long-term deficiency of an essential nutrient can lead to severe illness to death. Nutrition is a science that is a domain of knowledge consisting of systematically organized facts. Scientists have discovered the real nature of nutrition through experiments, and scientific development is quite fast, however, how the components of food are transformed into energy substances and nutrients required by human life and how the nutrients pass through the living cell walls is still a mystery palace to be explored by modern science.

Health lies in balanced nutrition, and the ministry of health of the state proposes: nutritional treatment will be the key to address chronic diseases. National ministry of health notice: setting up "clinical nutrition departments" by nationwide medical institutions means that the government first advances nutrition to therapeutic heights. 600 more than ten thousand doctors in the country receive nutrition education training, and doctors must hold nutrition qualification certificate to go on duty. It is also noted that medical treatments have less than 10% health promoting effect, while nutritional status accounts for 80%. At present, two or three hospitals in clinical nutrition departments and wards exist in China, and people need to perform nutrition screening, nutrition assessment and nutrition treatment, and clinical nutrition doctors prescribe nutrition treatment. Traditional nutrition recommends 0.8-1.0 g/kg. Day, and the recommendation for adult healthy people, based on a nitrogen balance measuring method, is very late and inaccurate in practice. The latest method is called indicator amino acid oxidation method, and the conclusion is 1.2 g/kg. Day, which is the lowest protein intake of healthy people and is more scientific and accurate than the former method. However, it is difficult to adapt to all people with a single reference value without considering the difference in digestibility between individual persons and the situation of aging persons. Therefore, the blood is recommended to be used by large households for detecting and monitoring the protein nutrition condition of the large households, and the amount of protein in the diet is adjusted to meet the needs of the body. When the sufficient amount of the protein is not up to the standard, the digestive function is weakened, the protein in the body can not be completely digested, and then the directly absorbed protein product must be eaten to balance the needs of the body, otherwise, the body must be damaged.

Blood protein detection standard: 65 g-85 g/L total protein, 40 g-55 g/L albumin, 20 g-40 g/L globulin, white ball ratio 1.2-2.4. Monitoring, evaluating and adjusting the protein nutrition balance of the body, which is necessary for health.

Animal and vegetable proteins are mentioned: the Canbel professor of the Cannel university in the United states is honored as einstein of the Nutrition kingdom, is a world nutriology authority, and was once a faithful apprentice of dairy products, meat products and animal proteins. The professor richarded fortunei of the great britain university, the institute of oncology of the chinese medical academy of sciences li, the professor liu boqi and the professor chen junshi, which is now the institute of chinese engineers, are scientists of the middle and american centre, and they jointly study the conclusion: animal proteins contain a variety of carcinogens and toxins, and animal proteins are carcinogenic. It is germinal and developing to cancer cells in vivo, especially milk protein containing 87% casein, which promotes the development of cancer cells at any stage in vivo, so it is recommended that milk protein be consumed in an amount of not more than 5% of total protein. The research conclusion is that: the plant protein is the most safe protein, and the plant food can reduce the occurrence of tumor. In addition, soy protein inhibits the development of cancer cells and is the best and safest protein. The most nutritious and high-quality meat, egg and milk are considered by the world as the strongest and most harmful health killers in history!

The American academy of consultation, "Special Committee for Nutrition problems", has a total of as many as five thousand pages, which surprises the Nutrition report in the United states! The meat of the 'eating meat is basically equivalent to the taking poison' contains a large amount of cadaveric poison, carcinogen, antibiotic and chemical toxin, a plurality of diseases of animals and the like.

During the korean war, 200 american soldiers in the military battle were necropsied at their cadavers and examined anatomically, and they were 22 years old on average, and it was found that 80% of them had hardened arteries and coagulated much fat. When corpses of korean soldiers of the same age were examined, it was found that their blood vessels were not hardened. The reason is that the food of American soldiers is mainly meat protein, and the food of Korea is mainly soybean vegetarian food, so that the food intake of animal protein can be controlled, and the occurrence of cardiovascular and cerebrovascular infarction can be prevented.

Teaching in campbell: "death" is caused by improper diet. If more than 60 g of animal protein is eaten every day, the carcinogenic factors are started, and if the intake is reduced to be less than 20g, the cancer focus can be controlled even if the cancer focus is started. Animal food promotes carcinogenesis. The plant food can reduce tumor occurrence, and the plant nutrient contains multiple enzyme and antioxidant, and the meat food is not. Meat does not represent nutrition, and when people eat many kinds of meat, hormones are generated in the bodies of the people, and the whole immune system is reduced. The immune system and plant nutrition are inseparable, nutritional, from the whole plant, and multiple species of plants represent nutrition.

China is not scientific at present on the basis of refined rice flour as a staple food, and the latest research results of molecular nutrition and physical doctor Sanshi and Korea Tanchuan Demei medical doctor: protein deficiency is a cause of chronic disease, and excessive intake of sugar is a source of all diseases. And indicates that the senile dementia is honored as the third diabetes and is a disease caused by excessive sugar intake and insufficient protein. The health of the balance of 45 essential nutrients in each period of life is very important, the plant protein does not contain cholesterol, does not promote the development of cancer cells, is safe high-quality protein, and must be supplemented with protein food which can be directly absorbed without digestion after the old age so as to ensure the balance of the protein nutrients. The reason why blue-eye, nasty euros now use brown rice, soy foods and vegetables as staple food diet programs, which all largely prove to be the healthiest dietary structure.

The invention aims to provide a plant high-quality protein product which is directly absorbed by the old without being digested, and solves the problem that the digestive function is reduced after aging, so that the protein cannot be completely digested, and the healthy big pain spots of the body which are not enough in protein for a long time are caused.

2. Provides mung bean protein products with high-quality detoxification function of plants which are not required to be directly absorbed by the market and high branched chain amino acid corn protein products with direct absorption and life-prolonging effects.

3. Provides food dipeptide and tripeptide products which are not needed to be digested in the market, and small molecular peptides are important substances for treating all diseases.

Central television station, science and education channel, discovery column, special report: peptide is a revolutionary product that will lead the human nutritional revolution in the 21 st century. The human has been through a total of three health revolution since the history, the first smallpox appeared, the solution was the vaccinium nikoense, the second penicillin was used to treat the disease caused by bacteria and virus, and the third was the use of small molecule active peptide to treat all diseases including all major diseases, namely the cell failure problem.

Doctz, a famous american biologist: small molecule peptide therapy will improve human longevity by at least 20 years. Doctor of the national academy of traditional medicine science, expert committee of the association of chinese medical experts, professor li chenesen: eight scientists in the world research a substance peptide to obtain the Nobel prize, and the most important substance for adjusting human bodies to be old and fatigued is the forefront of modern science, and if no peptide exists, no life exists.

At present, the directly absorbed protein product of the invention is not found in the market, and the product consists of small molecular dipeptide, tripeptide and compound amino acid. The science shows that: peptide products should be divided into food peptides and injection peptides. Insulin is the 51 peptide which must be injected into the body to exert the functional effects of the peptide. There are 400 dipeptides and 8000 tripeptides in terms of 18 amino acids, and the food peptide theoretically has 8400 functions and effects.

It is mentioned above that small molecule peptide is a revolutionary product, and the use of small molecule active peptide in treating all diseases including all major diseases, namely cell failure problem. How to supplement peptides is carried out in two ways: 1. small molecule di-and tripeptides are consumed. 2. The peptide is injected into the body by an injection method. The food peptide can be produced according to the soybean peptide standard without the food peptide standard in China, the product can only absorb a little, and the polypeptide larger than tripeptide enters the body and is not subjected to secondary enzymolysis basically, namely, the polypeptide cannot be digested for the second time and cannot be absorbed, so that the effect is very low, and the problem of the maximum peptide industry quality caused by the laggard enzyme industry technology is solved.

Science has demonstrated that: the human body can only directly absorb dipeptide, tripeptide and amino acid. However, it is difficult to limit the production of amino acids because the standard molecular weight of soybean peptide-derived products is less than 2000Da and more than 80%. The present invention is different in that the formation of amino acids is not limited, and the tripeptides are directly absorbed proteins. The results of the soybean peptide experiment in vitro, which is completed by professor of national institute of bioengineering and medical science of the liberty military, imitate the human stomach and trypsin, are completely consistent with the results reported by foreign documents, and the product can not be digested basically when entering the body, and a protective film is generated on the surface of the peptide and can not be acted by enzyme any more. Summary of soybean peptide functional verification reports: the results show that all functions of the domestic soybean peptide product are completely consistent with the results reported by foreign documents.

The molecular weight determination and in vitro digestion experiments of the soybean peptide are completed by the professor of national institute of bioengineering of the scientific institute of military medicine of the liberated military science, and the research results show that: the molecular weight of the soybean peptide produced by Shandong Zhongshi Duqing biotechnology limited company is lower than 1000, and the soybean peptide is mainly concentrated between 245 and 700, namely the soybean peptide mostly consists of 2 to 6 amino acids; after pepsin treatment, more than 95% of the soybean peptide is not digested, and after trypsin treatment, about 90% of the soybean peptide is not digested, which indicates that the product has good stability and can not be digested for the second time in vivo, and indicates that the peptide larger than tripeptide can not be utilized by human body after eating.

The human body needs to ensure health, two nutrients are required, one is that human body cells obtain enough nutrients, and the other is that the nutrients of beneficial flora for ensuring the intestinal microecology of the human body. And most importantly, reduce toxins in the body. The best way to reduce toxin is to eat mung bean protein, which can decompose hundreds of toxins and reduce vivotoxin.

University of california biology professor bruus. Ames' study, who said: 99.91% of the toxins ingested by humans are derived from the plant itself, a fact which is unknown to all. Only 0.1% is from pesticide residues sprayed on the plants. That is, the plants themselves carry chemical toxin weapons. Since the plant is not eaten by humans or other animals for its native purpose, it is also a growing purpose to survive. But they have no legs and cannot run out, so they have evolved some toxic substances to protect themselves. These phytotoxins are their defense weapons to protect themselves. The principle is the same as that of venom of a poisonous snake. Ames estimate says: the amount of natural toxins in humans is approximately 1.5 grams per day, which is a result of the consumption of plant foods. In the case of purely vegetarians, the daily intake of phytotoxins is greater. Therefore, the mung bean protein food must be eaten daily for detoxification in order to be healthy, which is very important for health.

The invention relates to a dipeptide tripeptide and amino acid product produced by vegetable protein and a preparation method thereof, and the invention contents are as follows: 1. the technological process of simulating human body's digestion of protein, i.e. acid and enzyme double-decomposition method, is innovated in vitro. Acid treatment at PH 1.8-3.5 and acid protease in the simulated stomach for 2-6 hours together, followed by conditioning, adjusting PH to 6.8-7.8, adding the complex papain, trypsin, oligopeptidase, carboxypeptidase, aminopeptidase complex protease in a ratio of 1.

2. For the first time, food peptides are combined with amino acid products to provide direct protein absorption, and food peptides, i.e. dipeptide and tripeptide products, are provided.

3. Four protein products which are directly absorbed by high-quality plants with different functions are innovated, soybean protein contains no cholesterol and is one of the best proteins, peanut protein has the name of pecans, the branched chain amino acid of corn protein has the highest content of 24.48 percent in all proteins, american journal of science, cell metabolism, cellMetabolism, published research results and reports of British daily telecommunication report that scientists find a substance capable of prolonging life, and experiments are carried out on mice for the first time, so that the protein products are successful. The miracle substance is a mixture of three amino acids, namely leucine, isoleucine and valine, and is known as branched chain amino acid or body strengthening amino acid. The branched chain amino acid' prolongs the life of the mouse by 12%, and meanwhile, the mouse body generates a series of positive physiological reactions, such as more energy taken up by cells, reduction of free radicals and the like. Free radicals are harmful compounds generated in oxidation reactions of the body, have strong oxidizing properties, and can damage tissues and cells of the body, thereby causing chronic diseases and aging effects. Through the change, the white mouse looks full of vitality, and the muscle coordination capacity is improved. Introduction by the investigator: "branched chain amino acids" are an important and effective nutritional supplement that helps the organism naturally, without side effects, strengthen the muscles and gain more energy. The main investigator of this study was professor Enzunesi of Milan university, italy, who also indicated that if a supplement of amino acids was invented on this basis, it would likely benefit mankind in the future. The mung bean protein product has unique characteristics, the activity of the mung bean protein product is basically not lost under the action of heating and strong acid of pH in the stomach, the mung bean protein product is a mechanism for detoxifying hundreds of toxins, and the development significance of the directly absorbed vegetable proteins is great.

The preparation process comprises the following steps:

(1) weighing the protein powder raw materials, adding water for mixing, adding hydrochloric acid into the mixture until the water content of the materials is between 90 and 100 percent to adjust the pH value of the materials to be 2.0 to 3.5, stirring the materials slowly for 5 to 7 hours at the temperature of between 40 and 50 ℃, and simultaneously adding acid protease with the addition amount of 0.3 to 1.5 percent of the weight of a substrate;

(2) mixing the feed liquid treated in the step (1) with water in an amount which is 1 to 8 to 10 times of the weight of the substrate, and carrying out size mixing treatment;

(3) adjusting the temperature of the raw material liquid treated in the step (2) to 54-56 ℃, adding 0.3-1.5% of compound protease for enzymolysis for 24-68 hours, wherein the pH of the material before enzymolysis is required to be 6.8-7.8, the temperature in the enzymolysis process is required to be controlled to be 54-56 ℃, and the enzymolysis process needs to be stirred at a low speed;

(4) carrying out inactivation protease treatment on the feed liquid subjected to enzymolysis in the step (3) at the temperature of more than 90 ℃ for 15 seconds and sterilization treatment on the feed liquid;

(5) blending the feed liquid obtained in the step (4) according to the standard of the liquid product, and performing aseptic filling to obtain a plant protein liquid product capable of being directly absorbed;

(6) and (5) concentrating the feed liquid in the step (4), and performing spray drying on the solid matter which reaches more than 40% to obtain a plant protein powder product capable of being directly absorbed.

The invention has the following advantages and positive effects:

1. the invention has the following positive effects: 1. obtaining the high-quality plant protein product which is directly absorbed and does not contain cholesterol. 2. The mung bean protein product with the function of detoxification and direct absorption is obtained. 3. The directly absorbed corn protein product with high branched chain amino acid and life prolonging effect is obtained. 4. Obtaining micromolecular food peptides with various physiological functions of micromolecular dipeptide and tripeptide;

2. in addition, china now has a minimum of 60 million sudden deaths per year, and pathological anatomy indicates that: a large proportion of this population are young adults who die not from obstruction of the myocardial infarction but from fatigue causing heart spasm.

Physiologically, fatigue is a result of catabolism, and in order to rapidly relieve fatigue, it is best to supplement protein within 30 minutes after fatigue, which can rapidly repair damaged cells and tissues. However, the macromolecular protein food can be absorbed and utilized after being digested for 3 to 4 hours at least after being eaten, and the digestion-free protein can be detected in vivo after being eaten for ten minutes on an empty stomach. From this point of view, the indigestible protein product is the best preventive food for eliminating heart spasm caused by fatigue, resulting in sudden death.

Detailed Description

Example 1

(1) Weighing 1000kg of soybean protein isolate powder raw material, adding water for mixing, adding hydrochloric acid into the mixture until the water content of the material is between 90 and 100 percent to adjust the pH value of the material to be 2.0, slowly stirring for 5 to 7 hours at the temperature of between 40 and 50 ℃, and simultaneously adding 5kg of acid protease accounting for the weight of the substrate;

(3) adjusting the temperature of the raw material liquid treated in the step (2) to 54-56 ℃, adding 5kg of compound protease, namely papain, trypsin, oligopeptidase, carboxypeptidase, aminopeptidase and compound protease, in a proportion of 1;

(5) blending the feed liquid obtained in the step (4) according to the standard of liquid products, and carrying out aseptic filling to obtain a soybean protein liquid product capable of being directly absorbed;

(6) concentrating the feed liquid in the step (4), and spray drying the solid matter of which the content is more than 40% to obtain the soybean protein powder product capable of being directly absorbed

Example 2

(1) Weighing 1000kg of mung bean protein powder raw material, adding water for mixing, adding hydrochloric acid into the mixture until the water content of the material is between 90 and 100 percent to adjust the pH value of the material to be 2.0, slowly stirring for 5 to 7 hours at the temperature of between 40 and 50 ℃, and simultaneously adding 5kg of acid protease accounting for the weight of the substrate;

(3) adjusting the temperature of the raw material liquid treated in the step (2) to 54-56 ℃, adding 5kg of compound protease, papain, trypsin, oligopeptidase, carboxypeptidase, aminopeptidase and compound protease in a ratio of 1;

(5) blending the feed liquid in the step (4) according to the standard of liquid products, and performing aseptic filling to obtain a mung bean protein liquid product capable of being directly absorbed;

(6) and (4) concentrating the feed liquid in the step (4), and spray-drying until the solid content reaches more than 40% to obtain a mung bean protein powder product capable of being directly absorbed.

Example 3

(1) Weighing 500kg of corn protein powder raw material, adding water for mixing, adding hydrochloric acid into the mixture until the water content of the material is between 90 and 100 percent to adjust the pH value of the material to be 2.0, slowly stirring for 5 to 7 hours at the temperature of between 40 and 50 ℃, and simultaneously adding 2.6kg of acid protease accounting for the weight of the substrate;

(3) adjusting the temperature of the raw material liquid treated in the step (2) to 54-56 ℃, adding 2.6kg of compound protease, papain, trypsin, oligopeptidase, carboxypeptidase, aminopeptidase and compound protease in a ratio of 1;

(4) inactivating protease in the feed liquid obtained after the enzymolysis in the step (3) at the temperature of more than 90 ℃ for 15 seconds and sterilizing the feed liquid;

(5) blending the feed liquid obtained in the step (4) according to the standard of liquid products, and carrying out aseptic filling to obtain a high branched chain amino acid corn protein liquid product which can be directly absorbed;

(6) and (4) concentrating the feed liquid in the step (4), and performing spray drying on solid substances of which the solid contents reach more than 40% to obtain a high branched chain amino acid corn protein powder product capable of being directly absorbed.

Example 4

(1) Weighing 500kg of peanut protein powder raw material, adding water for mixing, adding hydrochloric acid into the mixture until the water content of the material is between 90 and 100 percent to adjust the pH value of the material to be 2.0, slowly stirring for 5 to 7 hours at the temperature of between 40 and 50 ℃, and simultaneously adding 2.6kg of acid protease accounting for the weight of the substrate;

(2) mixing the feed liquid treated in the step (1) with water which is 8 to 10 times of the weight of the substrate according to the weight ratio of 1 to 10, and performing size mixing treatment;

(5) blending the feed liquid obtained in the step (4) according to the standard of liquid products, and carrying out aseptic filling to obtain a peanut protein liquid product which has the name of the pecans and can be directly absorbed;

(6) and (5) concentrating the feed liquid in the step (4), and performing spray drying on the solid matter which reaches more than 40% to obtain the peanut protein powder product which is called as the pecan and can be directly absorbed.

Claims

1. A method for producing dipeptide tripeptide and amino acid products by using plant protein and a preparation method thereof are characterized in that: soybean protein, corn protein, mung bean protein and peanut protein are used as raw materials, and an acid and enzyme double-hydrolysis process is adopted in vitro, namely the digestion process of a human body to the protein is simulated.

2. The compound protease is a compound enzyme such as papain, trypsin, oligopeptidase, carboxypeptidase and aminopeptidase which breaks the peptide bond of amino acid to obtain a protein product with the tripeptide below.

3. It consists of dipeptides and tripeptides and complex amino acids, and science has proved that: only this part of the protein can be directly absorbed without digestion.

4. The preparation process comprises the following steps:

weighing protein powder raw materials, adding water, mixing, adding hydrochloric acid to adjust the pH value of the material to be 2.0-3.5 when the water content of the material is 90-100%, stirring slowly for 5-7 hours at the temperature of 40-50 ℃, and simultaneously adding acidic protease, wherein the addition amount of the acidic protease is 0.3-1.5% of the weight of a substrate;

mixing the feed liquid treated in the step (1) with water in an amount which is 1 to 8 to 10 times of the weight of the substrate for size mixing treatment;

adjusting the temperature of the raw material liquid treated in the step (2) to 54-56 ℃, adding 0.3-1.5% of compound protease for enzymolysis for 24-68 hours, wherein the pH of the material in the enzymolysis is required to be 6.8-7.8, and the temperature in the enzymolysis process is required to be controlled to be 54-56 ℃ and is slowly stirred;

inactivating protease in the feed liquid after enzymolysis in the step (3) at the temperature of more than 90 ℃ for 15 seconds and sterilizing the feed liquid;

blending the feed liquid obtained in the step (4) according to the liquid product according to the standard, and performing aseptic filling to obtain a plant protein liquid product capable of being directly absorbed;

concentrating the feed liquid in the step (4), and spray drying until the solid content reaches more than 40% to obtain the plant protein powder product capable of being directly absorbed.

5. Secondly, the method for producing dipeptide tripeptide and amino acid products from plant protein and the preparation method thereof according to claim 1 are characterized in that: acid and enzyme double decomposition process, i.e. simulating the digestion process of human body to protein, the first step of regulating pH value of protein material to 2-3.5 with hydrochloric acid, adding acid proteinase and stirring for 5-7 hr.

6. And secondly, adding water for size mixing, simultaneously adjusting the pH value to 6.8-7.8, adding compound papain, trypsin, oligopeptidase, carboxypeptidase and aminopeptidase composite protease, wherein the ratio of the compound papain, trypsin, oligopeptidase, carboxypeptidase and aminopeptidase composite protease is 1.

7. Thirdly, the method for producing dipeptide tripeptide and amino acid products from plant protein and the preparation method according to claim 1, which is characterized in that: the vegetable protein is respectively soybean protein, corn protein, mung bean protein and peanut protein, and the four protein materials are used as protein raw materials to produce four directly absorbed vegetable protein products.