CN110536896A - 可溶性psgl-1蛋白质变体的纯化方法 - Google Patents

可溶性psgl-1蛋白质变体的纯化方法 Download PDF

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Publication number
CN110536896A
CN110536896A CN201880010814.XA CN201880010814A CN110536896A CN 110536896 A CN110536896 A CN 110536896A CN 201880010814 A CN201880010814 A CN 201880010814A CN 110536896 A CN110536896 A CN 110536896A
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CN
China
Prior art keywords
protein
buffer
concentration
chromatography
psgl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880010814.XA
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English (en)
Chinese (zh)
Inventor
F·马伊萨诺
F·克里韦林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bracco Suisse SA
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Bracco Suisse SA
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Application filed by Bracco Suisse SA filed Critical Bracco Suisse SA
Publication of CN110536896A publication Critical patent/CN110536896A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201880010814.XA 2017-02-09 2018-02-06 可溶性psgl-1蛋白质变体的纯化方法 Pending CN110536896A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17155366.2 2017-02-09
EP17155366 2017-02-09
PCT/EP2018/052889 WO2018146067A1 (en) 2017-02-09 2018-02-06 Process for the purification of soluble psgl-1 protein variants

Publications (1)

Publication Number Publication Date
CN110536896A true CN110536896A (zh) 2019-12-03

Family

ID=58016581

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880010814.XA Pending CN110536896A (zh) 2017-02-09 2018-02-06 可溶性psgl-1蛋白质变体的纯化方法

Country Status (5)

Country Link
US (1) US12516102B1 (https=)
EP (1) EP3580231A1 (https=)
JP (1) JP7065105B2 (https=)
CN (1) CN110536896A (https=)
WO (1) WO2018146067A1 (https=)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827817A (en) * 1992-10-23 1998-10-27 Genetics Institute, Inc. P-selectin ligand protein
WO2001072769A2 (en) * 2000-03-27 2001-10-04 Genetics Institute, Llc Methods for purifying highly anionic proteins
CN101541825A (zh) * 2006-08-28 2009-09-23 阿雷斯贸易股份有限公司 Fc融合蛋白的纯化方法
WO2014159441A1 (en) * 2013-03-14 2014-10-02 Amgen Inc. Removal of leaked affinity purification ligand

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6277975B1 (en) 1992-10-23 2001-08-21 Genetics Institute, Inc. Fusions of P-selectin ligand protein and polynucleotides encoding same
WO2003059935A2 (en) * 2001-12-21 2003-07-24 Immunex Corporation Methods for purifying protein
EP1736538A1 (en) 2005-06-21 2006-12-27 Cytos Biotechnology AG Process for the preparative purification of virus-like-particles (VLPs)
US20080274501A1 (en) 2007-05-02 2008-11-06 Chenming Zhang Method of purifying acidic proteins expressed in plants
EP2350130B1 (en) * 2008-10-31 2018-10-03 Wyeth LLC Purification of acidic proteins using ceramic hydroxyapatite chromatography
IL260957B2 (en) 2016-02-09 2023-11-01 Bracco Suisse Sa Chimeric, Recombinant Protein Glycoprotein Selectin-P Ligand-1 (PSGL-1) Conjugates, Preparations Containing Them and Uses Thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827817A (en) * 1992-10-23 1998-10-27 Genetics Institute, Inc. P-selectin ligand protein
WO2001072769A2 (en) * 2000-03-27 2001-10-04 Genetics Institute, Llc Methods for purifying highly anionic proteins
EP1681299A2 (en) * 2000-03-27 2006-07-19 Genetics Institute, LLC Methods for purifying highly anionic proteins
CN101541825A (zh) * 2006-08-28 2009-09-23 阿雷斯贸易股份有限公司 Fc融合蛋白的纯化方法
WO2014159441A1 (en) * 2013-03-14 2014-10-02 Amgen Inc. Removal of leaked affinity purification ligand

Also Published As

Publication number Publication date
US12516102B1 (en) 2026-01-06
JP2020506933A (ja) 2020-03-05
EP3580231A1 (en) 2019-12-18
WO2018146067A1 (en) 2018-08-16
JP7065105B2 (ja) 2022-05-11

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