CN110526777A - A kind of formula soil and preparation method thereof with prevention and treatment rice disease function - Google Patents
A kind of formula soil and preparation method thereof with prevention and treatment rice disease function Download PDFInfo
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- CN110526777A CN110526777A CN201910853936.1A CN201910853936A CN110526777A CN 110526777 A CN110526777 A CN 110526777A CN 201910853936 A CN201910853936 A CN 201910853936A CN 110526777 A CN110526777 A CN 110526777A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of formula soil and preparation method thereof with prevention and treatment rice disease function, and in particular to cultural control insect pest field.Based on parts by weight, formula soil includes 25~35 parts of plant charcoal, and 35~60 parts of clay, 10~20 parts of organic fertilizer and 2~8 parts of sustained release agent.Plant charcoal selected by the present invention has bacteria resistance function, can reduce the growth breeding of plant root disease microorganism.Meanwhile in added Activities of Some Plants charcoal, sufficient magnesium elements can be provided for rice plant, and cooperate organic fertilizer, can promote rice plant and carry out photosynthesis, the yield of paddy can be improved.It due to being also added with sustained release agent in the formula soil, may make antibacterial growth-promoting effect more preferably lasting, be more suitable the growth cycle of rice.
Description
Technical field
The present invention relates to cultural control insect pest field, it is specially a kind of with prevention and treatment rice disease function formula soil and
Preparation method.
Background technique
In modern agricultural production, pesticide refers to agriculturally for preventing and treating the chemical agent of pest and disease damage and coordinate plant growth,
Epidemic prevention that it is widely used in agriculture forest and husbandry production, environment and domestic hygiene are removed the evil, industrial goods it is mould proof with it is mothproof etc..Obviously, pesticide
There is irreplaceable role in modern agricultural production.
Traditional pesticide in use, has the drawback that (1) is easy to change the original composed structure of soil, unfavorable
In the friendly sustainable development of environment;(2) bad dispersibility is unfavorable for the absorption of plant;(3) it has a single function, peasant household is caused to need
Apply various medicaments.
For this purpose, providing that a kind of formulation rate is few and new soil formula that pest control effect is good is for assisting plantation farming
Object is technical staff's urgent problem to be solved.
Summary of the invention
The present invention provides a kind of formula soil and preparation method thereof with prevention and treatment rice disease function, the formula soil
Has the advantages that Shaoshi multiple-effect.
To achieve the above object, the invention provides the following technical scheme:
A kind of formula soil with prevention and treatment rice disease function, based on parts by weight, including 25~35 parts of plant charcoal, it sticks
35~60 parts of soil, 10~20 parts of organic fertilizer and 2~8 parts of sustained release agent.
Preferably, the plant charcoal is selected from cymose buckwheat rhizome stem charcoal, cocoanut charcoal, China tree charcoal, in cassava charcoal and corncob charcoal
At least one.
Preferably, organic fertilizer includes bio-fertilizer and is produced by isolated secondary metabolism in fungal secretion
Object.
Preferably, the fungi of isolated secondary metabolite is selected from Armillaria luteo-virens, Russula vivesscens (Schaeff.) Franch and mangrove
At least one of rhizosphere soil fungi.
Preferably, the bio-fertilizer is selected from least one of cow dung, pig manure and chicken manure.
Preferably, the sustained release agent is selected from carboxymethyl chitosan, CoFe2O4Graphene nanocomposite material and diatom
At least one of soil.
Preferably, the formula soil is based on parts by weight, including 35~60 parts of clay, and 5~15 parts of cymose buckwheat rhizome stem charcoal, coconut palm
3~8 parts of sub- charcoal, 5~10 parts of China tree charcoal, 2~8 parts of cassava charcoal, 3~12 parts of corncob charcoal, Armillaria luteo-virens secondary metabolite 5
~7 parts, 3~6 parts of Russula vivesscens (Schaeff.) Franch secondary metabolite, 5~12 parts of mangrove plant rhizosphere soil fungi secondary metabolite, cow dung 5
~15 parts, 4~9 parts of chicken manure and 3~6 parts of carboxymethyl chitosan.
Preferably, the formula soil is based on parts by weight, including 45 parts of clay, and 10 parts of cymose buckwheat rhizome stem charcoal, cocoanut charcoal 6
Part, 7 parts of China tree charcoal, 5 parts of cassava charcoal, 8 parts of corncob charcoal, 6 parts of Armillaria luteo-virens secondary metabolite, Russula vivesscens (Schaeff.) Franch secondary metabolism produces
5 parts of object, 8 parts of mangrove plant rhizosphere soil fungi secondary metabolite, 12 parts of cow dung, 7 parts of chicken manure and carboxymethyl chitosan 4
Part.
Wherein, cymose buckwheat rhizome stem charcoal, China tree charcoal, cassava charcoal have bacteria resistance function, can reduce plant root disease microorganism
Breed breeding.Meanwhile by the way that cocoanut charcoal is added in plant charcoal, its mixed air penetrability of various plants charcoal can be increased, can be increased
The entrance of oxygen prevents rice from rotten illness occur.Meanwhile corncob charcoal can provide sufficient magnesium member for rice plant
Element promotes rice plant to carry out photosynthesis, the yield of paddy can be improved.
Also, Armillaria luteo-virens secondary metabolite, Russula vivesscens (Schaeff.) Franch secondary metabolite, mangrove plant rhizosphere soil fungi time
The special terpene substances contained, after being absorbed by rice plant, are storable in the vacuole of cell in raw metabolite, not only to edible
The body of person is harmless, and long-time service can reduce the probability for suffering from inflammation, influenza.Meanwhile terpene substances are stored in vacuole, can make water
Rice plants give out certain " smell ", drive away the natural enemy of rice, prevent pest from biting Rice Leaf.
The present invention also provides a kind of preparation method of formula soil with prevention and treatment rice disease function, the preparation methods
Include the following steps:
(1) plant charcoal for accurately weighing preset weight number, pulverizes, and crosses 200 meshes, obtains plant charcoal mixed-powder.
(2) plant charcoal mixed-powder is uniformly mixed with clay, obtains thick soil.
(3) secondary metabolite of preset weight number fungi is taken to be uniformly mixed with sustained release agent, and in 35~40 DEG C of temperature
It is stirred continuously 60~90min of stir-frying under the conditions of degree, obtains embedded object.The step is the process of chelating, can make carboxymethyl chitosan
Small-molecule substance (secondary metabolite) embedding is got up, sustained release is played the role of.Meanwhile carboxymethyl chitosan sugar also has itself
There is fungistatic effect, rice plant illness can be effectively prevent.
Wherein, Armillaria luteo-virens secondary metabolite the preparation method comprises the following steps:
A, Armillaria luteo-virens are inoculated in LB culture medium and cultivate 45~60 days, filter to the secretion of Armillaria luteo-virens
Processing, obtains filtrate.
B, filtrate is mutually extracted through petroleum ether, and liquid separation after extraction obtains petroleum ether phase.
C, ethyl acetate is added in petroleum ether phase, liquid separation after extraction obtains ethyl acetate phase.
D, ethyl acetate phase is rotated to medicinal extract shape to get Armillaria luteo-virens secondary metabolite is arrived.
The preparation method of Russula vivesscens (Schaeff.) Franch secondary metabolite is same as above.
Mangrove plant rhizosphere soil fungi secondary metabolite is same as above.
(4) obtained embedded object is uniformly mixed with thick soil, the formula soil can be obtained.
Preferably, the temperature for stirring stir-frying is 37 DEG C in step (3), the time for stirring stir-frying is 80min.
The invention has the benefit that
Plant charcoal selected by the present invention has bacteria resistance function, can reduce the growth breeding of plant root disease microorganism.
Meanwhile in added Activities of Some Plants charcoal, sufficient magnesium elements can be provided for rice plant, and cooperate organic fertilizer, can promote
Rice plant carries out photosynthesis, and the yield of paddy can be improved.Due to being also added with sustained release agent in the formula soil, suppression may make
Bacterium growth-promoting effect is more preferably lasting, is more suitable the growth cycle of rice.
Specific embodiment
Embodiment 1
The present embodiment provides a kind of technical solutions:
A kind of formula soil with prevention and treatment rice disease function, based on parts by weight, which includes clay 35
Part, 5 parts of cymose buckwheat rhizome stem charcoal, 3 parts of cocoanut charcoal, 5 parts of China tree charcoal, 2 parts of cassava charcoal, 3 parts of corncob charcoal, Armillaria luteo-virens secondary metabolism
5 parts of product, 3 parts of Russula vivesscens (Schaeff.) Franch secondary metabolite, 5 parts of mangrove plant rhizosphere soil fungi secondary metabolite, 5 parts of cow dung, chicken
4 parts of excrement and 3 parts of carboxymethyl chitosan.
Wherein, above-mentioned Armillaria luteo-virens secondary metabolite the preparation method comprises the following steps:
(1), Armillaria luteo-virens are inoculated in LB culture medium and cultivate 45~60 days, and mycelia of being subject to covers with conical flask, to Huang
The secretion of green halimasch carries out suction filtration processing with Buchner funnel, obtains filtrate.
(2), filtrate is mutually extracted through petroleum ether, and liquid separation after extraction obtains petroleum ether phase.
(3), ethyl acetate is added in petroleum ether phase, liquid separation after extraction obtains ethyl acetate phase.
(4), ethyl acetate phase is rotated to medicinal extract shape to get Armillaria luteo-virens secondary metabolite is arrived.
The preparation method of Russula vivesscens (Schaeff.) Franch secondary metabolite is same as above.
Mangrove plant rhizosphere soil fungi secondary metabolite is same as above.
Meanwhile above-mentioned formula soil the preparation method comprises the following steps:
S01,5 parts of cymose buckwheat rhizome stem charcoal is accurately weighed, 3 parts of cocoanut charcoal, 5 parts of China tree charcoal, 2 parts of cassava charcoal, 3 parts of corncob charcoal,
It pulverizes, crosses 200 meshes, obtain plant charcoal mixed-powder.
S02, plant charcoal mixed-powder is uniformly mixed with clay, obtains thick soil.
S03,5 parts of Armillaria luteo-virens secondary metabolite are taken, 3 parts of Russula vivesscens (Schaeff.) Franch secondary metabolite, mangrove plant rhizosphere is native
5 parts of earth secondary fungus metabolite are uniformly mixed with 3 parts of carboxymethyl chitosan, and are stirred continuously and turn under the conditions of 37 DEG C of temperature
It fries 80min and obtains embedded object until the color of carboxymethyl chitosan is gradually dimmed.
S04, obtained embedded object is uniformly mixed with thick soil, the formula soil can be obtained.
In actual use, first the root up to not crossing rice seedling need to be discharged in water in rice seedlings field by above-mentioned formula soil,
The formula soil can be directly layed in the top of rice root by peasant household, keep rice field lower 24 hours without flowing water state
Normal irrigation or draining.
Embodiment 2
The present embodiment provides a kind of technical solutions:
A kind of formula soil with prevention and treatment rice disease function, based on parts by weight, which includes clay 45
Part, 10 parts of cymose buckwheat rhizome stem charcoal, 6 parts of cocoanut charcoal, 7 parts of China tree charcoal, 5 parts of cassava charcoal, 8 parts of corncob charcoal, Armillaria luteo-virens secondary generation
Thank 6 parts of product, 5 parts of Russula vivesscens (Schaeff.) Franch secondary metabolite, 8 parts of mangrove plant rhizosphere soil fungi secondary metabolite, 12 parts of cow dung,
7 parts of chicken manure and 4 parts of carboxymethyl chitosan.
The preparation method is the same as that of Example 1 for above-mentioned formula soil.
Embodiment 3
The present embodiment provides a kind of technical solutions:
A kind of formula soil with prevention and treatment rice disease function, based on parts by weight, which includes clay 55
Part, 12 parts of cymose buckwheat rhizome stem charcoal, 7 parts of cocoanut charcoal, 8 parts of China tree charcoal, 6 parts of cassava charcoal, 10 parts of corncob charcoal, Armillaria luteo-virens secondary generation
Thank 6 parts of product, 5 parts of Russula vivesscens (Schaeff.) Franch secondary metabolite, 10 parts of mangrove plant rhizosphere soil fungi secondary metabolite, cow dung 13
Part, 7 parts of chicken manure and 5 parts of carboxymethyl chitosan.
The preparation method is the same as that of Example 1 for above-mentioned formula soil.
Embodiment 4
The present embodiment provides a kind of technical solutions:
A kind of formula soil with prevention and treatment rice disease function, based on parts by weight, which includes clay 60
Part, 15 parts of cymose buckwheat rhizome stem charcoal, 8 parts of cocoanut charcoal, 10 parts of China tree charcoal, 8 parts of cassava charcoal, 12 parts of corncob charcoal, Armillaria luteo-virens are secondary
7 parts of metabolite, 6 parts of Russula vivesscens (Schaeff.) Franch secondary metabolite, 12 parts of mangrove plant rhizosphere soil fungi secondary metabolite, cow dung 15
Part, 9 parts of chicken manure and 6 parts of carboxymethyl chitosan.
The preparation method is the same as that of Example 1 for above-mentioned formula soil.
Comparative example 1 (no plant charcoal)
The present embodiment provides a kind of technical solutions:
A kind of formula soil with prevention and treatment rice disease function, based on parts by weight, which includes clay 45
Part, 6 parts of Armillaria luteo-virens secondary metabolite, 5 parts of Russula vivesscens (Schaeff.) Franch secondary metabolite, mangrove plant rhizosphere soil fungi secondary generation
Thank 8 parts of product, 12 parts of cow dung, 7 parts of chicken manure and 4 parts of carboxymethyl chitosan.
Comparative example 2 (no secondary fungus metabolite)
The present embodiment provides a kind of technical solutions:
A kind of formula soil with prevention and treatment rice disease function, based on parts by weight, which includes clay 45
Part, 10 parts of cymose buckwheat rhizome stem charcoal, 6 parts of cocoanut charcoal, 7 parts of China tree charcoal, 5 parts of cassava charcoal, 8 parts of corncob charcoal, 12 parts of cow dung, 7 parts of chicken manure
And 4 parts of carboxymethyl chitosan.
Comparative example 3 (without antibacterial sustained release agent)
A kind of formula soil with prevention and treatment rice disease function, based on parts by weight, which includes clay 45
Part, 10 parts of cymose buckwheat rhizome stem charcoal, 6 parts of cocoanut charcoal, 7 parts of China tree charcoal, 5 parts of cassava charcoal, 8 parts of corncob charcoal, Armillaria luteo-virens secondary generation
Thank 6 parts of product, 5 parts of Russula vivesscens (Schaeff.) Franch secondary metabolite, 8 parts of mangrove plant rhizosphere soil fungi secondary metabolite, 12 parts of cow dung
And 7 parts of chicken manure.
Test case 1
In order to verify formula soil provided by the invention to the control efficiency of rice sheath blight disease, technical staff has carried out as follows
Experiment:
In Rice Cropping base, chooses with suffering from 210 plants of banded sclerotial blight diseased plant in mu rice field, be randomly divided into 7 groups, number respectively
For A~G.Wherein, A~D group rice, which respectively corresponds to be laid with provided by Examples 1 to 4 in the root of rice, is formulated soil.E group
Corresponding to be formulated soil using comparative example 1 is provided, F group is corresponding using formula soil provided by comparative example 2, and G group correspondence makes
Soil is formulated provided by comparative example 3.After experiment 30 days, each group diseased plant situation such as table 1 is counted.Note: this test case is chosen
Rice plant plant height between 12~16cm.
1 rice diseased plant situation statistical form of table
Test case 2
In order to verify influence of the formula soil provided by the invention to rice yield, technical staff has carried out following experiment:
It in Rice Cropping base, chooses with 240 plants of rice plant in mu rice field, is randomly divided into 8 groups, number is A respectively
~H, every group of experimental plants are 30 plants.Wherein, A~D group rice, which is respectively corresponded, is laid with Examples 1 to 4 institute in the root of rice
The formula soil of offer.E group is corresponding using soil is formulated provided by comparative example 1, and F group is corresponding using provided by comparative example 2
It is formulated soil, G group is corresponding using soil is formulated provided by comparative example 3, and H group is not processed as control experiment group.Test water
After rice harvesting, each group paddy situation such as table 1 is counted.
2 Paddy rice output condition statistical form of table
Group/situation | Paddy total weight (kg) | Coarse Rice Rate (%) | Bad paddy rate |
A | 0.56 | 1.5 | 0.5 |
B | 0.65 | 0.5 | 0 |
C | 0.59 | 2 | 0 |
D | 0.60 | 1 | 1 |
E | 0.62 | 6 | 4 |
F | 0.45 | 4 | 2 |
G | 0.42 | 2 | 3 |
H | 0.39 | 10 | 6 |
Claims (10)
1. a kind of formula soil with prevention and treatment rice disease function, which is characterized in that based on parts by weight, including plant charcoal 25
~35 parts, 35~60 parts of clay, 10~20 parts of organic fertilizer and 2~8 parts of sustained release agent.
2. a kind of formula soil with prevention and treatment rice disease function according to claim 1, which is characterized in that the plant
Object charcoal is selected from cymose buckwheat rhizome stem charcoal, cocoanut charcoal, China tree charcoal, at least one of cassava charcoal and corncob charcoal.
3. a kind of formula soil with prevention and treatment rice disease function according to claim 1, which is characterized in that organic fertilizer
Including bio-fertilizer and pass through isolated secondary metabolite in fungal secretion.
4. a kind of formula soil with prevention and treatment rice disease function according to claim 3, which is characterized in that separate
To secondary metabolite fungi in Armillaria luteo-virens, Russula vivesscens (Schaeff.) Franch and mangrove plant rhizosphere soil fungi at least one
Kind.
5. a kind of formula soil with prevention and treatment rice disease function according to claim 3, which is characterized in that the life
Object fertilizer is selected from least one of cow dung, pig manure and chicken manure.
6. a kind of formula soil with prevention and treatment rice disease function according to claim 3, which is characterized in that described slow
It releases agent and is selected from carboxymethyl chitosan, CoFe2O4At least one of graphene nanocomposite material and diatomite.
7. a kind of formula soil with prevention and treatment rice disease function according to claims 1 to 6, which is characterized in that with
Parts by weight meter, including 35~60 parts of clay, 5~15 parts of cymose buckwheat rhizome stem charcoal, 3~8 parts of cocoanut charcoal, 5~10 parts of China tree charcoal, cassava
2~8 parts of charcoal, 3~12 parts of corncob charcoal, 5~7 parts of Armillaria luteo-virens secondary metabolite, Russula vivesscens (Schaeff.) Franch secondary metabolite 3~6
Part, 5~12 parts of mangrove plant rhizosphere soil fungi secondary metabolite, 5~15 parts of cow dung, 4~9 parts of chicken manure and methylol shell
3~6 parts of glycan.
8. a kind of formula soil with prevention and treatment rice disease function according to claim 7, which is characterized in that with weight
Number meter, including 45 parts of clay, 10 parts of cymose buckwheat rhizome stem charcoal, 6 parts of cocoanut charcoal, 7 parts of China tree charcoal, 5 parts of cassava charcoal, 8 parts of corncob charcoal,
6 parts of Armillaria luteo-virens secondary metabolite, 5 parts of Russula vivesscens (Schaeff.) Franch secondary metabolite, mangrove plant rhizosphere soil fungi secondary metabolism
8 parts of product, 12 parts of cow dung, 7 parts of chicken manure and 4 parts of carboxymethyl chitosan.
9. a kind of preparation method of the formula soil with prevention and treatment rice disease function, which comprises the steps of:
(1) plant charcoal for accurately weighing preset weight number, pulverizes, and crosses 200 meshes, obtains plant charcoal mixed-powder;
(2) plant charcoal mixed-powder is uniformly mixed with clay, obtains thick soil;
(3) secondary metabolite of preset weight number fungi is taken to be uniformly mixed with sustained release agent, and in 35~40 DEG C of temperature strip
It is stirred continuously 60~90min of stir-frying under part, obtains embedded object;
Wherein, Armillaria luteo-virens secondary metabolite the preparation method comprises the following steps:
A, Armillaria luteo-virens are inoculated in LB culture medium and cultivate 45~60 days, carry out at suction filtration to the secretion of Armillaria luteo-virens
Reason, obtains filtrate;
B, filtrate is mutually extracted through petroleum ether, and liquid separation after extraction obtains petroleum ether phase;
C, ethyl acetate is added in petroleum ether phase, liquid separation after extraction obtains ethyl acetate phase;
D, ethyl acetate phase is rotated to medicinal extract shape to get Armillaria luteo-virens secondary metabolite is arrived;
The preparation method of Russula vivesscens (Schaeff.) Franch secondary metabolite is same as above;
Mangrove plant rhizosphere soil fungi secondary metabolite is same as above;
(4) obtained embedded object is uniformly mixed with thick soil, the formula soil can be obtained.
10. a kind of preparation method of formula soil with prevention and treatment rice disease function according to claim 9, feature
It is, in step (3), the temperature for stirring stir-frying is 37 DEG C, and the time for stirring stir-frying is 80min.
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Cited By (1)
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CN112358346A (en) * | 2020-11-09 | 2021-02-12 | 宁夏大学 | Special fertilizer for pressed muskmelon in vine extending period and application thereof |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN112358346A (en) * | 2020-11-09 | 2021-02-12 | 宁夏大学 | Special fertilizer for pressed muskmelon in vine extending period and application thereof |
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Application publication date: 20191203 |