CN110506888A - For removing detoxifying agent, the preparation method and application of mycotoxin - Google Patents
For removing detoxifying agent, the preparation method and application of mycotoxin Download PDFInfo
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- CN110506888A CN110506888A CN201910777047.1A CN201910777047A CN110506888A CN 110506888 A CN110506888 A CN 110506888A CN 201910777047 A CN201910777047 A CN 201910777047A CN 110506888 A CN110506888 A CN 110506888A
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- mycotoxin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/273—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption using adsorption or absorption agents, resins, synthetic polymers, or ion exchangers
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
Abstract
The detoxifying agent that the present invention relates to a kind of for removing mycotoxin, preparation method and application, the detoxifying agent for being used to remove mycotoxin is made of each raw material of following parts by weight: hydration sial calcium disodium: 1 ~ 2 part, bacillus subtilis: 0.5 ~ 0.7 part, yeast powder: 0.5 ~ 0.7 part, yeast cell wall: 28 ~ 30 parts, montmorillonite: 63 ~ 66 parts, phytase: 3 ~ 5 parts, benzoic acid: 0.1 ~ 0.3 part, manna oligosacchride and-glucan are wherein included at least in yeast cell wall.The detoxifying agent proposed by the present invention for being used to remove mycotoxin, can effectively remove the mycotoxin in feed, improve the safety and feed effect of formula forage, meet practical raising demand.
Description
Technical field
The present invention relates to field of feed additive technology, in particular to a kind of detoxifying agent for removing mycotoxin, system
Preparation Method and application.
Background technique
It is well known that feed is essential important source material in breeding technology field.For feed, all kinds of feeds
In raw material and all kinds of compound feed products, inevitably can all there be mycotoxin contamination, recall rate is even up to
70%~100%.
For food pollution, 7~8 kinds of Duo Zeyou, a variety of moulds are presented in few then 2~3 kinds of detection toxin for polluting feed
The feature of toxin combined pollution.In view of the particularity of mycotoxin, to fungus growth breeding regulation and mould condition, mycotoxin are produced
Metabolic residue rule, toxicity of mycotoxins and its machine of distribution and development law, mycotoxin in animal body in feed
Fast and accurately detection device and technology detecting method etc. are required to for system, the reciprocal effects of a variety of mycotoxins and mycotoxin
It carries out going deep into system research.Since mycotoxin has biggish harmfulness, it is therefore desirable to be removed to it.Currently, mould
Detoxicated dose of product mainly includes 2 major class of mold toxin sorbent and biological enzymolysis toxic agent, and mold toxin sorbent according to
The difference of its component can be divided into active carbon class, mannans, Aluminosilicates and biological species etc..Toxin is added in feed to inhale
Attached dose is the mycotoxin eliminating feed method being most widely used in current Production of Livestock and Poultry.
However, existing toxin agent has also adsorbed minerals, the vitamin in feed while absorbing mycotoxin
Etc. nutritional ingredients, influence the absorption of nutriment.
Summary of the invention
Based on this, the purpose of the present invention is to solve existing toxin agent also to adsorb while absorbing mycotoxin
Minerals, vitamins and other nutritious components in feed, the problem of affecting the absorption of nutriment.
The present invention proposes a kind of for removing the detoxifying agent of mycotoxin, wherein the detoxifying agent is prepared from the following raw materials:
It is hydrated sial calcium disodium, bacillus subtilis, yeast powder, yeast cell wall, montmorillonite, phytase and benzoic acid;
Raw material needed for wherein preparing the detoxifying agent of Unit Weight includes following each components by weight percent:
Hydration sial calcium disodium: 1 ~ 2 part;Bacillus subtilis: 0.5 ~ 0.7 part;
Yeast powder: 0.5 ~ 0.7 part;Yeast cell wall: 28 ~ 30 parts:
Montmorillonite: 63 ~ 66 parts;Phytase: 3 ~ 5 parts;
Benzoic acid: 0.1 ~ 0.3 part;
Manna oligosacchride and-glucan are wherein included at least in the yeast cell wall.
The detoxifying agent proposed by the present invention for being used to remove mycotoxin, by hydration sial calcium disodium, bacillus subtilis, ferment
The raw materials such as female powder, yeast cell wall, montmorillonite, phytase and benzoic acid are made, wherein hydration sial calcium disodium, it can be to including
Mycotoxin adsorption capacity with super strength including aflatoxin;In yeast cell wall-glucan ingredient, have efficient
The mycotoxin (such as zearalenone) for adsorbing low pole, using bacillus subtilis conversion technology by Gibberella zeae
Ketenes is converted into nontoxic metabolin, strengthens detoxicating functions;Manna oligosacchride in yeast cell wall can be colonized to be tied on stomach surface
Protective film is formed on coincidence point, prevents the infringement of harmful bacteria, and is excreted in conjunction with the harmful bacterias such as Escherichia coli in enteron aisle,
Manna oligosacchride and bacillus subtilis promote the production and breeding of beneficial bacterium in animal body simultaneously, to inhibit the life of harmful bacteria
It is long, improve function of intestinal canal and health;Oligosaccharides and marine algae extract synergistic effect in yeast cell wall, intensify immunity of organism machine
Meeting enhances Immune Function In Animals.To sum up, above-mentioned detoxifying agent targetedly can effectively remove the toxin in feed,
Without the absorption of the nutriments such as influence minerals therein, vitamin.
The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent for preparing Unit Weight is corresponding each heavy
Measuring component includes following component:
Hydration sial calcium disodium: 1 part;Bacillus subtilis: 0.5 part;
Yeast powder: 0.5 part;Yeast cell wall: 28 parts:
Montmorillonite: 63 parts;Phytase: 5 parts;
Benzoic acid: 0.1 part;
Wherein, the bacillus subtilis is YB4.
The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent for preparing Unit Weight is corresponding each heavy
Measuring component includes following component:
Hydration sial calcium disodium: 2 parts;Bacillus subtilis: 0.7 part;
Yeast powder: 0.7 part;Yeast cell wall: 30 parts:
Montmorillonite: 66 parts;Phytase: 5 parts;
Benzoic acid: 0.1 part;
Wherein, the bacillus subtilis is YB4.
The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent for preparing Unit Weight is corresponding each heavy
Measuring component includes following component:
Hydration sial calcium disodium: 1.5 parts;Bacillus subtilis: 0.6 part;
Yeast powder: 0.6 part;Yeast cell wall: 29.6 parts:
Montmorillonite: 65 parts;Phytase: 4 parts;
Benzoic acid: 0.2 part;
Wherein, the bacillus subtilis is YB4.
The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent further includes bacillus coagulans and fibre
Tie up plain enzyme, the corresponding parts by weight of the bacillus coagulans are 0.5 ~ 1 part, and the corresponding weight fraction of the cellulase is
1.2 ~ 1.5 parts.
The detoxifying agent for being used to remove mycotoxin, wherein the detoxifying agent for preparing Unit Weight is corresponding each heavy
Measuring component includes following component:
Hydration sial calcium disodium: 1.5 parts;Bacillus subtilis: 0.6 part;
Yeast powder: 0.6 part;Yeast cell wall: 29.6 parts:
Montmorillonite: 65 parts;Phytase: 4 parts;
Benzoic acid: 0.2 part;Bacillus coagulans: 0.5 part;
Cellulase: 1.3 parts;
Wherein, the bacillus subtilis is YB4.
The present invention also proposes a kind of for removing the preparation method of the detoxifying agent of mycotoxin, wherein the detoxifying agent is
Detoxifying agent as described above, the preparation method include the following steps:
Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de-
Toxic agent raw material slurry;
Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, is added and steams
Distilled water, 15 DEG C ~ 20 DEG C at a temperature of stand 12 ~ 20h obtain active bacteria mixed liquor;
Step 3: the active bacteria mixed liquor is added in the detoxifying agent raw material slurry, after mixing evenly at 25 DEG C ~ 30 DEG C
At a temperature of stand 3 ~ 5h, then in the environment of 35 DEG C ~ 45 DEG C stand until moisture be evaporated to obtain the detoxifying agent;
Manna oligosacchride and-glucan are wherein included at least in the yeast cell wall.
The preparation method of the detoxifying agent for being used to remove mycotoxin, wherein prepare the detoxifying agent of Unit Weight
Required raw material includes following each components by weight percent:
Hydration sial calcium disodium: 1 ~ 2 part;Bacillus subtilis: 0.5 ~ 0.7 part;
Yeast powder: 0.5 ~ 0.7 part;Yeast cell wall: 28 ~ 30 parts:
Montmorillonite: 63 ~ 66 parts;Phytase: 3 ~ 5 parts;
Benzoic acid: 0.1 ~ 0.3 part.
The preparation method of the detoxifying agent for being used to remove mycotoxin, wherein the active bacteria mixing is being prepared
After the step of liquid, the method also includes:
Bacillus coagulans and cellulase are continuously added into the active bacteria mixed liquor, it is quiet after at the uniform velocity 10 ~ 15min of stirring
2 ~ 3h is set, and the mixed liquor obtained after standing is poured into the detoxifying agent raw material slurry and is stirred.
The present invention also proposes a kind of for removing the application of the detoxifying agent of mycotoxin, wherein application is as described above to be used
In removing mycotoxin detoxifying agent the mycotoxin in feed is removed, the mycotoxin include aflatoxin,
At least one of zearalenone, vomitoxin and fumarubicin.
Additional aspect and advantage of the invention will be set forth in part in the description, and will partially become from the following description
Obviously, or practice through the invention is recognized.
Specific embodiment
To facilitate the understanding of the present invention, below with reference to related embodiment to invention is more fully described.Implement
Preferred embodiment of the invention is given in example.But the invention can be realized in many different forms, however it is not limited to this
Literary described embodiment.On the contrary, purpose of providing these embodiments is make it is more thorough to the disclosure complete
Face.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term " and or " used herein includes one or more phases
Any and all combinations of the listed item of pass.
Existing toxin agent has also adsorbed the nutrition such as minerals, the vitamin in feed while absorbing mycotoxin
Ingredient influences the absorption of nutriment.
In order to solve the above-mentioned technical problem, the present invention proposes a kind of for removing the detoxifying agent of mycotoxin, wherein this is de-
Toxic agent is prepared from the following raw materials:
It is hydrated sial calcium disodium, bacillus subtilis, yeast powder, yeast cell wall, montmorillonite, phytase and benzoic acid.
Specifically, raw material needed for preparing the detoxifying agent of Unit Weight includes following each heavy in actual fabrication process
Measure component:
Hydration sial calcium disodium: 1 ~ 2 part;Bacillus subtilis: 0.5 ~ 0.7 part;
Yeast powder: 0.5 ~ 0.7 part;Yeast cell wall: 28 ~ 30 parts:
Montmorillonite: 63 ~ 66 parts;Phytase: 3 ~ 5 parts;
Benzoic acid: 0.1 ~ 0.3 part.
It should be added that, for above-mentioned yeast cell wall, including at least in yeast cell wall has herein
Manna oligosacchride and-glucan.
The detoxifying agent proposed by the present invention for being used to remove mycotoxin, by hydration sial calcium disodium, bacillus subtilis, ferment
The raw materials such as female powder, yeast cell wall, montmorillonite, phytase and benzoic acid are made, wherein hydration sial calcium disodium, it can be to including
Mycotoxin adsorption capacity with super strength including aflatoxin;In yeast cell wall-glucan ingredient, have efficient
The mycotoxin (such as zearalenone) for adsorbing low pole, using bacillus subtilis conversion technology by Gibberella zeae
Ketenes is converted into nontoxic metabolin, strengthens detoxicating functions;Manna oligosacchride in yeast cell wall can be colonized to be tied on stomach surface
Protective film is formed on coincidence point, prevents the infringement of harmful bacteria, and is excreted in conjunction with the harmful bacterias such as Escherichia coli in enteron aisle,
Manna oligosacchride and bacillus subtilis promote the production and breeding of beneficial bacterium in animal body simultaneously, to inhibit the life of harmful bacteria
It is long, improve function of intestinal canal and health;Oligosaccharides and marine algae extract synergistic effect in yeast cell wall, intensify immunity of organism machine
Meeting enhances Immune Function In Animals.To sum up, above-mentioned detoxifying agent targetedly can effectively remove the toxin in feed,
Without the absorption of the nutriments such as influence minerals therein, vitamin.
At the same time, the present invention also proposes a kind of for removing the preparation method of the detoxifying agent of mycotoxin, wherein described
Detoxifying agent is detoxifying agent as described above, and the preparation method includes the following steps:
Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de-
Toxic agent raw material slurry.
Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, added
Enter distilled water, 15 DEG C ~ 20 DEG C at a temperature of stand 12 ~ 20h obtain active bacteria mixed liquor.
Step 3: the active bacteria mixed liquor is added in the detoxifying agent raw material slurry, after mixing evenly 25 DEG C ~ 30
3 ~ 5h is stood at a temperature of DEG C, is then stood in the environment of 35 DEG C ~ 45 DEG C until moisture is evaporated to obtain the detoxifying agent.
It it should be added that, include herein sweet dew in yeast cell wall for above-mentioned yeast cell wall
Oligosaccharides and-glucan.
Specific embodiments of the present invention are discussed in further detail with several specific embodiments below:
Embodiment one:
The preparation method for the detoxifying agent for removing mycotoxin that first embodiment of the invention proposes, specifically includes following step
It is rapid:
Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de-
Toxic agent raw material slurry.
Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, added
Enter distilled water, 15 DEG C at a temperature of stand 12h obtain active bacteria mixed liquor.
In this step, above-mentioned bacillus subtilis is YB4.
Step 3: the active bacteria mixed liquor is added in the detoxifying agent raw material slurry, after mixing evenly at 25 DEG C
At a temperature of stand 3h, then in the environment of 35 DEG C stand until moisture be evaporated to obtain detoxifying agent.
In the present embodiment, it as shown in following table one, prepares each composition by weight added by every kilogram of detoxifying agent and is respectively as follows:
Table one:
Embodiment two:
The preparation method for the detoxifying agent for removing mycotoxin that second embodiment of the invention proposes, specifically includes following step
It is rapid:
Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de-
Toxic agent raw material slurry.
Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, added
Enter distilled water, 20 DEG C at a temperature of stand 20h obtain active bacteria mixed liquor.
In this step, above-mentioned bacillus subtilis is YB4.
Step 3: the active bacteria mixed liquor is added in the detoxifying agent raw material slurry, after mixing evenly at 30 DEG C
At a temperature of stand 5h, then in the environment of 45 DEG C stand until moisture be evaporated to obtain the detoxifying agent.
It it should be added that, include herein sweet dew in yeast cell wall for above-mentioned yeast cell wall
Oligosaccharides and-glucan.
In the present embodiment, it as shown in following table two, prepares each composition by weight added by every kilogram of detoxifying agent and is respectively as follows:
Table two:
Embodiment three:
The preparation method for the detoxifying agent for removing mycotoxin that third embodiment of the invention proposes, specifically includes following step
It is rapid:
Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de-
Toxic agent raw material slurry.
Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, added
Enter distilled water, 18 DEG C at a temperature of stand 17h obtain active bacteria mixed liquor.
In this step, above-mentioned bacillus subtilis is YB4.
Step 3: the active bacteria mixed liquor is added in the detoxifying agent raw material slurry, after mixing evenly at 27 DEG C
At a temperature of stand 4h, then in the environment of 40 DEG C stand until moisture be evaporated to obtain the detoxifying agent.
It it should be added that, include herein sweet dew in yeast cell wall for above-mentioned yeast cell wall
Oligosaccharides and-glucan.
In the present embodiment, it as shown in following table three, prepares each composition by weight added by every kilogram of detoxifying agent and is respectively as follows:
Example IV:
The preparation method for the detoxifying agent for removing mycotoxin that fourth embodiment of the invention proposes, specifically includes following step
It is rapid:
Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de-
Toxic agent raw material slurry.
Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, added
Enter distilled water, 17 DEG C at a temperature of stand 18h obtain active bacteria mixed liquor.
In this step, above-mentioned bacillus subtilis is YB4.
Step 3: continuously adding bacillus coagulans and cellulase into active bacteria mixed liquor, at the uniform velocity stirring 14min
After stand 2.5h, and the mixed liquor obtained after standing is poured into detoxifying agent raw material slurry and is stirred.
In the present invention, during preparing detoxifying agent, the corresponding parts by weight of the bacillus coagulans of addition are 0.5
~ 1 part, the corresponding weight fraction of the cellulase of addition is 1.2 ~ 1.5 parts.
Step 4: by active bacteria mixed liquor be added to detoxifying agent raw material slurry in, after mixing evenly 25 DEG C at a temperature of it is quiet
3h is set, is then stood in the environment of 35 DEG C until moisture is evaporated to obtain detoxifying agent.
In the present embodiment, it as shown in following table four, prepares each composition by weight added by every kilogram of detoxifying agent and is respectively as follows:
Table four:
Embodiment five
Fifth embodiment of the invention also proposes a kind of for removing the application of the detoxifying agent of mycotoxin, wherein applies institute as above
The detoxifying agent for removing mycotoxin stated removes the mycotoxin in feed, and the mycotoxin includes aspergillus flavus
Toxin, zearalenone, vomitoxin and fumarubicin.
For the feed that goes mouldy, wherein mycotoxin content are as follows: aflatoxin B1/2.7, zearalenone/899,
Vomitoxin/1429, macrofusine/5803.
The detoxifying agent being prepared with the present invention handles mildew feed, to the mold toxin sorbent of main mycotoxin
Adsorption ratio as shown in following table five:
Table five: mold toxin sorbent of the detoxifying agent to main mycotoxin
Absorptivity | Embodiment one | Embodiment two | Embodiment three | Example IV | Comparative example |
Aflatoxin | 100% | 98.33% | 96.25% | 99.85% | 0% |
Zearalenone | 92.36% | 91.25% | 93.11% | 92.56% | 0% |
Vomitoxin | 68.17% | 66.55% | 67.19% | 67.27% | 0% |
Fumarubicin | 70.58% | 71.36% | 70.20% | 69.56% | 0% |
It can be seen that the detoxifying agent that the present invention is prepared from table five, to aflatoxin, the Gibberella zeae in mildew feed
Ketenes, vomitoxin and fumarubicin can effectively be absorbed.
As supplement, the detoxifying agent being prepared using the present invention is added in feed, is fed to weanling pig
It supports, test result are as follows: after the detoxifying agent of 0.1% this product of addition, weight, which improves 1.2 kilograms/7%, FCR, reduces by 0.27/13%;Add
After the detoxifying agent for adding 2.5% this product, weight, which improves 2.3/13%, FCR, reduces by 0.41/18%.
This product is detected through Jiangxi Province's veterinary drug feed check, in product main component, saccharomyces cerevisiae viable count >=1,
000,000,000/g, bacillus subtilis clump count >=100,000,000CFU/g.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of for removing the detoxifying agent of mycotoxin, which is characterized in that the detoxifying agent is prepared from the following raw materials:
It is hydrated sial calcium disodium, bacillus subtilis, yeast powder, yeast cell wall, montmorillonite, phytase and benzoic acid;
Raw material needed for wherein preparing the detoxifying agent of Unit Weight includes following each components by weight percent:
Hydration sial calcium disodium: 1 ~ 2 part;Bacillus subtilis: 0.5 ~ 0.7 part;
Yeast powder: 0.5 ~ 0.7 part;Yeast cell wall: 28 ~ 30 parts:
Montmorillonite: 63 ~ 66 parts;Phytase: 3 ~ 5 parts;
Benzoic acid: 0.1 ~ 0.3 part;
Manna oligosacchride and-glucan are wherein included at least in the yeast cell wall.
2. according to claim 1 for removing the detoxifying agent of mycotoxin, which is characterized in that prepare the institute of Unit Weight
Stating the corresponding each composition by weight of detoxifying agent includes following component:
Hydration sial calcium disodium: 1 part;Bacillus subtilis: 0.5 part;
Yeast powder: 0.5 part;Yeast cell wall: 28 parts:
Montmorillonite: 63 parts;Phytase: 5 parts;
Benzoic acid: 0.1 part;
Wherein, the bacillus subtilis is YB4.
3. according to claim 1 for removing the detoxifying agent of mycotoxin, which is characterized in that prepare the institute of Unit Weight
Stating the corresponding each composition by weight of detoxifying agent includes following component:
Hydration sial calcium disodium: 2 parts;Bacillus subtilis: 0.7 part;
Yeast powder: 0.7 part;Yeast cell wall: 30 parts:
Montmorillonite: 66 parts;Phytase: 5 parts;
Benzoic acid: 0.1 part;
Wherein, the bacillus subtilis is YB4.
4. according to claim 1 for removing the detoxifying agent of mycotoxin, which is characterized in that prepare the institute of Unit Weight
Stating the corresponding each composition by weight of detoxifying agent includes following component:
Hydration sial calcium disodium: 1.5 parts;Bacillus subtilis: 0.6 part;
Yeast powder: 0.6 part;Yeast cell wall: 29.6 parts:
Montmorillonite: 65 parts;Phytase: 4 parts;
Benzoic acid: 0.2 part;
Wherein, the bacillus subtilis is YB4.
5. according to claim 1 for removing the detoxifying agent of mycotoxin, which is characterized in that the detoxifying agent further includes
Bacillus coagulans and cellulase, the corresponding parts by weight of the bacillus coagulans are 0.5 ~ 1 part, the cellulase
Corresponding weight fraction is 1.2 ~ 1.5 parts.
6. according to claim 5 for removing the detoxifying agent of mycotoxin, which is characterized in that prepare the institute of Unit Weight
Stating the corresponding each composition by weight of detoxifying agent includes following component:
Hydration sial calcium disodium: 1.5 parts;Bacillus subtilis: 0.6 part;
Yeast powder: 0.6 part;Yeast cell wall: 29.6 parts:
Montmorillonite: 65 parts;Phytase: 4 parts;
Benzoic acid: 0.2 part;Bacillus coagulans: 0.5 part;
Cellulase: 1.3 parts;
Wherein, the bacillus subtilis is YB4.
7. a kind of for removing the preparation method of the detoxifying agent of mycotoxin, which is characterized in that the detoxifying agent is such as above-mentioned power
Benefit requires detoxifying agent described in 1 to 6 any one, and the preparation method includes the following steps:
Step 1: being stirred mixing for montmorillonite, yeast cell wall and yeast powder, and then addition distilled water stirs to get de-
Toxic agent raw material slurry;
Step 2: hydration sial calcium disodium, bacillus subtilis, phytase and benzoic acid are mixed, is added and steams
Distilled water, 15 DEG C ~ 20 DEG C at a temperature of stand 12 ~ 20h obtain active bacteria mixed liquor;
Step 3: the active bacteria mixed liquor is added in the detoxifying agent raw material slurry, after mixing evenly at 25 DEG C ~ 30 DEG C
At a temperature of stand 3 ~ 5h, then in the environment of 35 DEG C ~ 45 DEG C stand until moisture be evaporated to obtain the detoxifying agent;
Manna oligosacchride and-glucan are wherein included at least in the yeast cell wall.
8. according to claim 7 for removing the preparation method of the detoxifying agent of mycotoxin, which is characterized in that preparation is single
Raw material needed for the detoxifying agent of position weight includes following each components by weight percent:
Hydration sial calcium disodium: 1 ~ 2 part;Bacillus subtilis: 0.5 ~ 0.7 part;
Yeast powder: 0.5 ~ 0.7 part;Yeast cell wall: 28 ~ 30 parts:
Montmorillonite: 63 ~ 66 parts;Phytase: 3 ~ 5 parts;
Benzoic acid: 0.1 ~ 0.3 part.
9. according to claim 7 for removing the preparation method of the detoxifying agent of mycotoxin, which is characterized in that preparing
After the step of obtaining the active bacteria mixed liquor, the method also includes:
Bacillus coagulans and cellulase are continuously added into the active bacteria mixed liquor, it is quiet after at the uniform velocity 10 ~ 15min of stirring
2 ~ 3h is set, and the mixed liquor obtained after standing is poured into the detoxifying agent raw material slurry and is stirred.
10. a kind of for removing the application of the detoxifying agent of mycotoxin, which is characterized in that application such as the claims 1 to 6
The mycotoxin in feed is removed for removing the detoxifying agent of mycotoxin described in meaning one, the mycotoxin packet
Include at least one of aflatoxin, zearalenone, vomitoxin and fumarubicin.
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