CN114831248A - Biological mildew removing agent and preparation method and application thereof - Google Patents
Biological mildew removing agent and preparation method and application thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
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- A23K20/163—Sugars; Polysaccharides
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- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
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- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
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- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/273—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption using adsorption or absorption agents, resins, synthetic polymers, or ion exchangers
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Abstract
The application relates to the technical field of animal feed additives, and particularly discloses a biological mildew removing agent and a preparation method and application thereof. A biological mildew removing agent comprises the following raw materials: active beneficial bacteria, yeast cell walls, modified hydrated aluminosilicates, plant extracts and biological enzymes; the active probiotic bacteria comprise lactobacillus reuteri; the plant extract comprises radix Arnebiae extract; the biological enzyme comprises glucose oxidase. The biological mildew removal agent feed obtained by adding the biological mildew removal agent feed is fed, so that the average weight gain and the average daily feed intake of breeding sows are respectively 0.31kg and 0.71kg, the feed-meat ratio is 2.32, and the albumin and the globulin are respectively 43.54g/L and 27.65 g/L; in addition, the daily average weight gain and the daily average feed intake of the broilers are 60.90g and 88.31g respectively, the feed-meat ratio is 1.45, the Newcastle disease antibody titer is 10.48, and the production performance and the immunity of breeding sows and the broilers are improved.
Description
Technical Field
The application relates to the field of animal feed additives, in particular to a biological mold removal agent and a preparation method and application thereof.
Background
For livestock and poultry breeding, mycotoxin contamination is possible during the processes of feed raw material collection, product processing, storage, transportation, sale and use. The main mycotoxins in the feed are vomitoxin, aflatoxin, zearalenone, ochratoxin, fumonisin and the like, and a plurality of mycotoxins coexist in the feed, and have synergistic effect and strong toxic action among the toxins. For example, aflatoxin and vomitoxin, ochratoxin and toxin, vomitoxin and zearalenone all have synergistic effects, and when the toxins simultaneously appear, the toxicity is far higher than that of a single mycotoxin. After animals are fed with the feed infected by mycotoxin, the feed causes serious harm to the health of livestock, can damage the liver and the kidney of the animals, and can block the normal metabolism and the immunity of the animals, and even can cause carcinogenesis and teratogenesis.
In the related art, in order to reduce the damage of mycotoxin to the animal body, the mildew removing agent is usually added into animal feed to be mixed and fed, most of the mildew removing agent is singly adsorbed, and can adsorb nutrition while adsorbing mildew, so that part of nutrition in the feed is lost, and the immune system of an animal is still not obviously improved after the feed containing the mildew removing agent is eaten for a long time.
Disclosure of Invention
In order to solve the problem that mycotoxin destroys the immune system of animals, the application provides a biological mildew removing agent and a preparation method and application thereof.
In a first aspect, the present application provides a biological mold remover, which adopts the following technical scheme:
a biological mildew removing agent comprises the following raw materials in parts by weight: 70-90 parts of active beneficial bacteria, 10-30 parts of yeast cell wall, 270 parts of modified hydrated aluminosilicate 250-290 parts, 290 parts of plant extract 270-290 parts and 370 parts of biological enzyme 350-370 parts; the modified hydrated aluminosilicate is obtained by modifying cetyl pyridinium chloride and cetyl trimethyl ammonium bromide; the active beneficial bacteria comprise lactobacillus reuteri; the plant extract comprises radix Arnebiae extract; the biological enzyme comprises glucose oxidase.
In the application, the biological mildew removing agent is prepared from 70-90 parts of active beneficial bacteria, 10-30 parts of yeast cell walls, 270 parts of modified hydrated aluminosilicate 250-one, 290 parts of plant extracts and 370 parts of biological enzymes 350-one, and the animal performance of feeding the feed added with the biological mildew removing agent is expected, and the effect is optimal when the active beneficial bacteria is 80 parts, the yeast cell walls are 20 parts, the hydrated aluminosilicate 260 parts, the plant extracts are 280 parts and the biological enzymes are 360 parts.
By adopting the technical scheme, the active beneficial bacteria can effectively inhibit the growth of the mould and reduce the generation of mycotoxin through the biological metabolism effect of the active beneficial bacteria, and can be combined with the mycotoxin to form a peptidoglycan compound to be discharged out of a body to play a role in detoxification. Lactobacillus reuteri is selected as active probiotics to be added, so that the lactobacillus reuteri can bear the corrosion of gastric acid, secretes adhesin per se, can smoothly propagate on the cell surface in large quantities, and further has the beneficial effects of regulating the intestinal ecological environment and the like. In addition, lactobacillus reuteri can generate lactic acid and various enzymes such as lipase and bile salt hydrolase in animal intestinal tracts, and is beneficial to improving the pH of the animal intestinal tracts, inhibiting the growth of mold, improving the utilization rate of feed and the like. In addition, the lactobacillus reuteri can synthesize B vitamins, improve the growth performance of livestock and poultry and maintain the health of animals. Secondly, the lactobacillus reuteri can improve the immunity of animals, resist apoptosis, stimulate the maturation of immune organs of organisms and promote the organisms to resist infection.
The yeast cell wall is rich in various bioactive substances such as beta-glucan, mannan and the like, the active substances can be used as immunostimulating factors to regulate the immunologic function of animal organisms, so that the function of enhancing the immunity is achieved, and the yeast cell wall has the effects of enhancing the immunity, preventing and treating diseases, promoting growth, relieving stress and supplementing nutrition, and can absorb mould and remove toxin by being added as an adsorbent. In addition, the yeast cell wall also has the function of maintaining the balance of intestinal flora.
The hydrated aluminosilicate is a three-dimensional framework structure compound consisting of silicon oxygen and aluminum tetrahedron, belongs to a cubic crystal system, has larger specific surface area and ion adsorption capacity, and plays a role in adsorbing mycotoxin. The modification treatment of hydrated aluminosilicate is carried out by adopting cetyl pyridinium chloride and cetyl trimethyl ammonium bromide, the molecular pore size of the aluminosilicate is changed, the capability of adsorbing mycotoxin by the hydrated aluminosilicate is improved, and the hydrated aluminosilicate has the function of directional adsorption.
The plant extract can destroy the cell wall of mold hyphae and spore, destroy the cell structure of mold to degrade mold or inhibit mold propagation, thereby relieving mycotoxin production in the feed. In addition, the addition of the plant extract can improve the detoxification capability of animal liver to mycotoxin. The radix Arnebiae extract can effectively enhance rat serum activity caused by carbon tetrachloride and reduce serum bilirubin content, and has effects of resisting liver cell injury, protecting liver and recovering liver function, thereby improving animal liver detoxicating ability to mycotoxin.
The addition of the biological enzyme can stimulate the gastrointestinal tract of animals to produce detoxification enzyme, induce the detoxification capability of organisms, destroy the structure of mycotoxin, generate nontoxic degradation products and eliminate the toxicity. Meanwhile, the degradation of biological enzyme is utilized to degrade mycotoxin, destroy mycotoxin or reduce mycotoxin, so as to reduce the damage of mycotoxin to animals. The glucose oxidase can catalyze the glucose in the intestinal tract to generate gluconic acid and hydrogen peroxide, the reaction can consume the oxygen in the intestinal tract, an anaerobic environment is created for the proliferation of lactic acid bacteria, when the glucose oxidase enters the digestive tract of animals, a part of the glucose oxidase enters the liver, and the oxidation-reduction reaction in the liver is accelerated, so that the metabolism of toxic components is accelerated, the drug poisoning of livestock and poultry is relieved, the growth of aspergillus flavus is inhibited, the aflatoxin is degraded, and the glucose oxidase also has the effect of regulating the balance of the gastrointestinal flora of the animals.
Preferably, the method comprises the following steps: a biological mildew removing agent comprises the following raw materials in parts by weight: 75-85 parts of active beneficial bacteria, 15-25 parts of yeast cell wall, 255-265 parts of hydrated aluminosilicate, 285 parts of plant extract and 355-365 parts of biological enzyme.
In the application, the biological mildew removing agent is prepared from 75-85 parts of active beneficial bacteria, 15-25 parts of yeast cell walls, 255-265 parts of hydrated aluminosilicate, 275-285 parts of plant extracts and 355-365 parts of biological enzymes, the animal performance of feeding the feed added with the biological mildew removing agent is expected, and the effect is optimal when 80 parts of the active beneficial bacteria, 20 parts of the yeast cell walls, 260 parts of the hydrated aluminosilicate, 280 parts of the plant extracts and 360 parts of the biological enzymes are used.
Preferably, the method comprises the following steps: the modified hydrated aluminosilicate is prepared by the following steps: slowly adding hydrated aluminum silicate salt into deionized water, uniformly stirring, and mixing the mixture according to the volume ratio of the total dosage of cetylpyridinium chloride and cetyltrimethylammonium bromide to the hydrated aluminum silicate salt of 1: (25-50) adding cetyl pyridinium chloride and cetyl trimethyl ammonium bromide according to the proportion, stirring, shearing, drying, and carrying out superfine grinding to obtain modified hydrated aluminum silicate; the weight ratio of the cetyl pyridinium chloride to the cetyl trimethyl ammonium bromide is 1: (1-3).
By adopting the scheme, the hydrated aluminum silicate is slowly added into the deionized water, so that the hydrated aluminum silicate is conveniently dispersed in the deionized water, and the hydrated aluminum silicate is modified by cetyl pyridinium chloride and cetyl trimethyl ammonium bromide to change the pore diameter of the hydrated aluminum silicate, so that the hydrated aluminum silicate has directional adsorption capacity, the biological mildew removing agent only adsorbs mycotoxin, nutrient substances are not adsorbed, and the condition that part of nutrition is lost due to the addition of the biological mildew removing agent into the feed can be avoided.
Preferably, the method comprises the following steps: the active beneficial bacteria also comprise the following raw materials in parts by weight: 20-30% of lactobacillus reuteri, 30-50% of bacillus licheniformis, 20-30% of bacillus subtilis, 1-3% of lactobacillus rhamnosus and 5-7% of lactic acid bacteria.
By adopting the technical scheme, the bacillus licheniformis can adjust the dysbacteriosis, recover the intestinal function, promote the organism to generate antibacterial active substances and kill pathogenic bacteria. Meanwhile, the bacillus licheniformis can also generate an anti-active substance, has a unique biological oxygen-deprivation action mechanism, inhibits the growth and the propagation of mould fungi, and reduces the mycotoxin in the feed from the source.
Active substances such as subtilin, polymyxin, nystatin, gramicidin and the like generated in the growth process of the bacillus subtilis have obvious inhibition effect on mould or pathogenic bacteria with endogenous infection. In addition, the bacillus subtilis quickly consumes free oxygen in the intestinal tract, so that the intestinal tract hypoxia is caused, the growth of beneficial anaerobic bacteria is promoted, organic acids such as lactic acid and the like are generated, the pH value of the intestinal tract is reduced, and the growth of moulds is indirectly inhibited. Meanwhile, the bacillus subtilis can stimulate the growth and development of immune organs of animals, activate lymphocytes, improve the levels of immunoglobulin and antibody, enhance the functions of cellular immunity and humoral immunity, improve the immunity of the animals, actively synthesize various trace elements such as various B-type vitamins and the like, promote the degradation of nutrients in the feed, enable the animals to fully absorb the nutrients in the feed and improve the conversion rate of the feed.
The lactobacillus rhamnosus has higher adsorption capacity on aflatoxin, can prevent an animal organism from absorbing the aflatoxin, and is discharged out of the body together with the mycotoxin; in addition, the lactobacillus rhamnosus has the function of improving the intestinal microecology of animal organisms and can avoid the phenomenon that the animal organisms generate diarrhea due to mycotoxin. The cell wall of the lactobacillus has specific binding sites with aflatoxin and vomitoxin, so that the binding of the mycotoxin to an intestinal wall intima receptor can be reduced, and the gastrointestinal discomfort of an animal body caused by the mycotoxin can be reduced; in addition, the toxin is brought out of the animal body through the action of the specific binding site of the lactobacillus cell wall and the mycotoxin, and the aflatoxin and the vomitoxin can be better removed.
Preferably, the method comprises the following steps: the biological enzyme comprises the following raw materials in percentage by weight: 35-40% of glucose oxidase, 20-30% of phytase, 10-25% of catalase and 12-27% of superoxide dismutase.
By adopting the technical scheme, the phytase can increase the nutritional titer of mineral elements, can decompose natural organic phosphorus in animal feed, and can release calcium ions which can participate in crosslinking or other reactions, thereby changing the texture of vegetable food. Meanwhile, the phytase can decompose mycotoxin in intestinal tracts and reduce damage of the mycotoxin to animal organisms.
The catalase oxidizes the mycotoxin by utilizing hydrogen peroxide, so that the mycotoxin is changed into a nontoxic substance, and simultaneously, the hydrogen peroxide is further changed into nontoxic water, so that immune organs such as livers and kidneys of animal organisms are effectively protected and repaired, and the problem that the animal immune system is damaged by the mycotoxin is effectively solved.
Superoxide dismutase can specifically remove harmful free radicals in vivo, so as to relieve organism damage caused by oxidizing certain components in vivo by free radicals, and improve immunity of animal organism.
Preferably, the method comprises the following steps: the plant extract also comprises the following raw materials in percentage by weight: 30-50% of lithospermum extract, 10-20% of silybum marianum extract, 15-30% of schisandra extract and 20-30% of seaweed extract.
By adopting the technical scheme, the silybum marianum extract can clear active oxygen, free radicals in animal bodies are important factors causing liver problems, the peroxidation process of fatty compounds can be blocked in time, the cell membrane fluidity and healthy physiological functions of liver cells are maintained, and the resistance of cell membranes to various damage factors is enhanced; and the silybum marianum can enhance the generation of glutathione, promote the detoxification reaction of the second stage of the liver and enhance the detoxification capability of the liver, and the silybum marianum mainly contains a flavonol compound, and the flavonol compound silybin has obvious protective effect on liver injury caused by various liver readings, thereby achieving the purpose of protecting the liver.
The seaweed extract can promote regeneration of injured mucosa epithelium of digestive tract, recover normal rhythm of intestinal peristalsis, maintain intestinal looseness and absorption, and improve immunity of digestive tract.
The fructus Schisandrae extract has liver protecting effect, and can promote biosynthesis of protein and glycogen of liver cell, promote regeneration and repair of liver cell, promote enzyme activity, and improve liver detoxication ability.
Preferably, the method comprises the following steps: the biological mildew removing agent also comprises 0.5-1 weight part of esterified glucomannan and 15-30 weight parts of mannan oligosaccharide.
By adopting the technical scheme, the esterified glucomannan has good physicochemical properties of low heat, stability, safety, no toxicity and the like, and also has the effects of protecting intestinal tracts, improving immunity and the like. The mannanoligosaccharide can eliminate the harmful effect of toxin on organism by physical adsorption or direct mycotoxin.
In a second aspect, the present application provides a method for preparing any one of the above biological mold-removing agents, which is specifically realized by the following technical scheme:
a preparation method of a biological mildew removing agent comprises the following operation steps:
preparing modified hydrated aluminosilicate for later use;
under the aseptic condition, uniformly mixing the raw materials of the biological mildew removing agent to obtain a mixture A;
mixing the mixture A with deionized water in a volume ratio of 1: and (3) uniformly mixing the components in the proportion of (3-5), drying and crushing to obtain the biological mildew removing agent.
In a third aspect, the present application provides a use of any one of the above biological mold release agents in animal feed.
In summary, the present application includes at least one of the following beneficial technical effects:
(1) according to the method, by controlling the raw material variety and the mixing amount of the biological mildew removal agent, the average weight gain and the daily average feed intake of the breeding sows are respectively 0.20kg and 0.49kg, the feed-meat ratio is 2.49, and the albumin and the globulin are respectively 43.20g/L and 26.12g/L, so that the production performance and the immunity of the breeding sows are improved; in addition, the daily average weight gain and the daily average feed intake of the broiler chickens are respectively 52.09g and 77.62g, the feed-meat ratio is 1.49, the Newcastle disease antibody titer is 9.88, and the production performance and the immunity of the broiler chickens are also improved.
(2) According to the application, bacillus licheniformis, bacillus subtilis, lactobacillus rhamnosus and lactobacillus are added into active beneficial bacteria raw materials of the biological mildew removing agent, and the mixing amount of the bacillus licheniformis, the bacillus subtilis, the lactobacillus rhamnosus and the lactobacillus is controlled, so that the average weight gain and the daily average feed intake of breeding sows are respectively 0.23kg and 0.57kg, the feed-meat ratio is 2.45, and the albumin and the globulin are respectively 43.27g/L and 27.0 g/L; in addition, the daily average weight gain and the daily average feed intake of the broilers are 53.78g and 79.59g respectively, the feed-meat ratio is 1.48, the Newcastle disease antibody titer of the broilers is 9.98, and the production performance and the immunity of breeding sows and the broilers are further improved.
(3) The method comprises the steps of adding phytase, catalase and superoxide dismutase into biological enzyme raw materials of the biological mildew removal agent, and controlling the mixing amount of the phytase, the catalase and the superoxide dismutase to ensure that the average weight gain and the average daily feed intake of breeding sows are respectively 0.26kg and 0.64kg, the feed-meat ratio is 2.42, and the albumin and the globulin are respectively 43.36g/L and 27.24 g/L; in addition, the daily average weight gain and the daily average feed intake of the broilers are 56.33g and 82.81g respectively, the feed-meat ratio is 1.47, the Newcastle disease antibody titer is 10.15, and the production performance and the immunity of breeding sows and the broilers are further improved.
(4) The silybum marianum extract, the schisandra chinensis extract and the seaweed extract are added into the biological mildew removing agent plant extract raw materials, and the mixing amount is controlled, so that the average weight gain and the daily average feed intake of breeding sows are respectively 0.28kg and 0.66kg, the feed-meat ratio is 2.39, and the albumin and the globulin are respectively 43.45g/L and 27.40 g/L; in addition, the daily average weight gain and the daily average feed intake of the broilers are 58.42g and 85.30g respectively, the feed-meat ratio is 1.46, the Newcastle disease antibody titer is 10.35, and the production performance and the immunity of breeding sows and the broilers are further improved.
(5) The method comprises the steps of adding esterified glucomannan and mannan-oligosaccharide into a biological mildew removal agent, and controlling the mixing amount of the esterified glucomannan and mannan-oligosaccharide to ensure that the average weight gain and the average daily feed intake of breeding sows are respectively 0.31kg and 0.71kg, the feed-meat ratio is 2.32, and the albumin and globulin are respectively 43.54g/L and 27.65 g/L; in addition, the daily average weight gain and the daily average feed intake of the broilers are 60.90g and 88.31g respectively, the feed-meat ratio is 1.45, the Newcastle disease antibody titer is 10.48, and the production performance and the immunity of breeding sows and the broilers are improved.
Detailed Description
The present application will be described in further detail with reference to specific examples. The following raw materials are all food grade commercial products, all of which are fully disclosed herein, and should not be construed as limiting the source of the raw materials. The method specifically comprises the following steps: yeast cell wall with 99% of effective substance content; hydrated aluminosilicate, type X-13; lactobacillus reuteri with a bacteria content of 100 hundred million CFU/g; radix Arnebiae extract with a product number of REL0062 and a particle size of 120 meshes; glucose oxidase with the enzyme activity of 200U/g; the bacillus licheniformis has the bacterium content of 1000 hundred million CFU/g; the bacillus subtilis has the bacterium content of 1000 hundred million CFU/g; lactobacillus rhamnosus, the bacterium content is 100 hundred million CFU/g; lactic acid bacteria with a bacteria content of 100 hundred million CFU/g; phytase with enzyme activity of 30000U/g; catalase with an enzymatic activity of 100000U/g; superoxide dismutase, the enzyme activity is 20000U/g; silybum marianum extract with particle size of 120 meshes; fructus Schisandrae extract, 80 mesh; seaweed extract with particle size of 120 meshes; esterified glucomannan, the content of active substance is 90%, mannan and glucose are bonded by beta-1, 4 bond, the molar ratio of mannan to glucose is 1.6: (1-4); the mannan oligosaccharide has the polymerization degree of 2-10 and the product number of BH-20220407002.
Example 1
The biological mold remover of example 1, which is prepared by the following procedure:
slowly adding hydrated aluminum silicate salt into deionized water, uniformly stirring, and mixing the mixture according to the volume ratio of the total dosage of cetylpyridinium chloride and cetyltrimethylammonium bromide to the hydrated aluminum silicate salt of 1: adding cetylpyridinium chloride and cetyltrimethylammonium bromide according to the proportion of 40, wherein the weight ratio of the cetylpyridinium chloride to the cetyltrimethylammonium bromide is 1: 2 stirring, shearing, drying and micronizing to obtain modified hydrated aluminum silicate with the particle size of 200 meshes for later use;
uniformly mixing active beneficial bacteria (lactobacillus reuteri), yeast cell walls, modified hydrated aluminosilicate, plant extracts (lithospermum extracts) and biological enzymes (glucose oxidase) according to the mixing amount of the table 1 under an aseptic condition to obtain a mixture A; mixing with deionized water, and drying to obtain biological mildew removing agent.
Examples 2 to 3
The preparation methods and the types of the raw materials of the biological mildew removing agents of the examples 2 to 3 are completely the same as those of the preparation method and the types of the raw materials of the example 1, and the specific details are shown in the table 1.
TABLE 1 blending amounts (unit: kg) of raw materials of the biological mildew removing agents of examples 1 to 3
| Raw materials | Example 1 | Example 2 | Example 3 |
| Active beneficial bacteria | 80 | 80 | 80 |
| Yeast cell wall | 20 | 20 | 20 |
| Modified hydrated aluminosilicates | 250 | 260 | 270 |
| Plant extracts | 8 | 8 | 8 |
| Biological enzymes | 10 | 10 | 10 |
Examples 4 to 6
The preparation method and the raw material mixing amount of the biological mildew removing agent in the embodiment 4-6 are the same as the embodiment 2, except that the active beneficial bacteria raw material is added with bacillus licheniformis, bacillus subtilis, lactobacillus rhamnosus and lactic acid bacteria, the types of the other raw materials are completely the same as the embodiment 2, and the specific mixing amount is shown in the table 2. .
TABLE 2 blending amounts (unit: kg) of active beneficial bacteria in the raw materials of the biological mildew-removing agents of examples 4-6
| Raw materials | Example 4 | Example 5 | Example 6 |
| Lactobacillus reuteri | 25 | 25 | 25 |
| Bacillus licheniformis | 43 | 42 | 41 |
| Bacillus subtilis | 25 | 25 | 25 |
| Lactobacillus rhamnosus | 2 | 2 | 2 |
| Lactic acid bacteria | 5 | 6 | 7 |
Examples 7 to 11
The methods for preparing the biological mold removing agents of examples 7-11 and the amounts of the raw materials were the same as those of example 5, except that phytase, catalase and superoxide dismutase were added to the raw materials of the biological enzymes, and the types of the other raw materials were the same as those of example 5, and the specific amounts are shown in Table 3.
TABLE 3 blending amounts (unit: kg) of biological enzymes in the raw materials of biological mildewcides of examples 7 to 11
Examples 12 to 16
The preparation method and the raw material mixing amount of the biological mold remover in the examples 12-16 are the same as those in the example 10, except that the silybum marianum extract, the schisandra chinensis extract and the seaweed extract are added into the plant extract raw materials, the types of the other raw materials are completely the same as those in the example 10, and the specific mixing amount is shown in the table 4.
TABLE 4 blending amount (unit: kg) of each raw material of plant extracts in the raw materials of the biological mildewcide of examples 12 to 16
Examples 17 to 21
The preparation method and the raw material mixing amount of the biological mold remover in the examples 17-21 are the same as those in the example 15, except that the silybum marianum extract, the schisandra chinensis extract and the seaweed extract are added into the plant extract raw material, the types of the other raw materials are completely the same as those in the example 15, and the specific mixing amount is shown in table 5.
TABLE 5 blending amounts (unit: kg) of the raw materials of the biological mildew removing agents of examples 17 to 21
Comparative example 1
The biomildewcide of comparative example 1 is prepared in exactly the same manner as in example 1, except that: the modified hydrated aluminosilicate was replaced with the hydrated aluminosilicate in the same amount, and the other raw materials and blending amounts were the same as in example 1.
Comparative example 2
The biological mildew removing agent of the comparative example 2 is exactly the same as the preparation method of the example 1, except that: the hydrated aluminosilicate obtained by modification of cetylpyridinium chloride and cetyltrimethylammonium bromide is replaced by the hydrated aluminosilicate obtained by modification of cetylpyridinium chloride, and the specific operation of modification of the hydrated aluminosilicate obtained by modification of cetylpyridinium chloride is as follows: slowly adding hydrated aluminum silicate salt into deionized water, uniformly stirring, and mixing cetyl pyridinium chloride and hydrated aluminum silicate salt according to the volume ratio of 1: 10, stirring, shearing, drying and micronizing to obtain a modified hydrated aluminum silicate salt, wherein the rest raw materials and the mixing amount are the same as those in the example 1.
Comparative example 3
The biological mildew removing agent of the comparative example 3 is exactly the same as the preparation method of the example 1, except that: the hydrated aluminosilicate obtained by modification of cetylpyridinium chloride and cetyltrimethylammonium bromide is replaced by the hydrated aluminosilicate obtained by modification of cetyltrimethylammonium bromide, and the specific operation of modification of the hydrated aluminosilicate obtained by modification of cetyltrimethylammonium bromide is as follows: slowly adding hydrated aluminum silicate salt into deionized water, uniformly stirring, and mixing cetyl trimethyl ammonium bromide and hydrated aluminum silicate salt according to the volume ratio of 1: 10, stirring, shearing, drying and micronizing to obtain the modified hydrated aluminum silicate salt, wherein the rest raw materials and the mixing amount are the same as those in the example 1.
Comparative example 4
The biomildewcide of comparative example 4 is prepared in exactly the same manner as in example 1, except that: the lactobacillus reuteri is replaced by bacillus natto in equal amount, and the other raw materials and the mixing amount are the same as those in the example 1.
Comparative example 5
The biomildewcide of comparative example 5 is prepared in exactly the same manner as in example 1 except that: the extract of lithospermum erythrorhizon was replaced with the extract of aloe in the same amount, and the other raw materials and the blending amount were the same as in example 1.
Comparative example 6
The biomildewcide of comparative example 6 is prepared in exactly the same manner as in example 1 except that: the same amount of glucose oxidase was replaced with cellulase, and the remaining raw materials and the amount of the mixture were the same as in example 1.
The following is the application of the biological mildew removing agent in the pig feed
Application example I-1
From the beginning of the gestation period of the sows to the end of the weaning period, the biological mildew removing agent obtained in the example 1 is added into the feed for breeding the sows according to the addition amount of 2 kg/ton, the sows are all fed by adopting a limit fence, and the breeding mode is carried out by adopting an artificial insemination mode. 2.25kg of feed is fed every day in the nonpregnant period, 2kg of feed is fed in 20 days after hybridization, the feeding amount of 2.25kg is recovered in 21 days after hybridization until 80 days of pregnancy, and the feeding amount is increased to 3kg when 81 days of pregnancy begin.
Application examples I-2 to I-21
The application examples I-2 to I-21 are the same as the application example I-1 in the application method, except that the biological mold remover obtained in the example 2 to 21 is used.
Comparative examples I-1 to I-6
The application comparative examples I-1 to I-6 are the same as the application example I-1 except that the biological mold remover obtained in the comparative examples 1 to 6 is used as the biological mold remover.
Performance detection
The following detection standards or methods are adopted to respectively carry out performance detection on different application examples I-1 to I-21 and application comparative examples I-1 to I-6, and the detection results are shown in Table 6 in detail.
270 breeding sows are selected and averagely divided into 27 groups, 10 breeding sows in each group are fed with the feed obtained by the application examples I-1-I-21 and the application comparative examples I-1-I-6 respectively, the breeding sows in each group are continuously fed for 181 days under the same feeding condition, the initial weight and the tested weight of the breeding sows in each group are detected, the average weight gain, the daily average feed intake and the feed-meat ratio are calculated, then the blood drawing is carried out on the 27 breeding sows after the test, the contents of albumin and globulin in the blood are detected, and the specific detection results are shown in Table 6.
TABLE 6 results of performance tests of different biological mildew-removing agents
The detection results in the table 6 show that the average daily gain and average daily feed intake of the breeding sows fed with the biological mildew removal agent feed obtained by the application are 0.31kg and 0.71kg respectively, the feed conversion ratio is 2.32 at least, and the albumin and globulin of the breeding sows are 43.54g/L and 27.65g/L respectively at most, which shows that the production performance of the breeding sows can be improved and the immunity of the breeding sows is improved by feeding the biological mildew removal agent feed obtained by the application.
In application examples I-1 to I-3, the daily average feed intake of the sow bred in application example I-2 is 0.49kg, which is higher than that of application example I-1 and application example I-3, the feed-meat ratio is 2.49, which is lower than that of application example I-1 and application example I-3, and the albumin and globulin of the sow bred in application example I-2 are 43.20g/L and 26.1g/L, which are higher than that of application example I-1 and application example I-3, respectively, which shows that the modified hydrated aluminum silicate in the biological mildew remover in application example I-2 is suitable in mixing amount, the production performance and immunity of the bred sow are improved, and the hydrated aluminum silicate can be modified by cetyl pyridinium chloride and cetyl trimethyl ammonium bromide to change the aperture of the hydrated aluminum silicate, so that the hydrated aluminum silicate has directional adsorption capacity, the biological mildew removing agent only adsorbs mycotoxin and does not adsorb nutrient substances, so that the condition that partial nutrition is lost due to the addition of the biological mildew removing agent in the feed can be avoided.
In application examples I-4 to I-6, the average daily gain and the average daily feed intake of the breeding sows of the application example I-5 are respectively 0.23kg and 0.57kg which are higher than those of the application example I-4 and the application example I-6, the feed-meat ratio is 2.45 which is lower than that of the application example I-4 and the application example I-6, the albumin and globulin of the sow bred in the application example I-5 are respectively 43.27g/L and 27.0g/L which are higher than those of the application example I-4 and the application example I-6, which shows that the lactic acid bacteria in the active beneficial bacteria of the biological degermation agent in the application example I-5 are mixed properly, the production performance and the immunity of the bred sow are improved, possibly, the specific binding sites with aflatoxin and vomitoxin are arranged on the cell wall of the lactobacillus, so that the binding of the mycotoxin to an intestinal wall intima receptor can be reduced, and the gastrointestinal discomfort of an animal body caused by the mycotoxin is reduced; in addition, the toxin is brought out of the animal body through the action of the specific binding site of the cell wall of the lactobacillus and the mycotoxin, and the correlation between the aflatoxin and the vomitoxin can be better removed.
In application examples I-7 to I-11, the average weight gain and daily average feed intake of the sow bred in application example I-10 are respectively 0.26kg and 0.64kg, which are higher than those in application examples I-7 to I-9 and I-11, the feed-meat ratio is 2.42, which is lower than those in application examples I-7 to I-9 and I-11, and the albumin and globulin of the sow bred in application example I-10 are respectively 43.36g/L and 27.24g/L, which are higher than those in application examples I-7 to I-9 and I-11, which shows that the catalase and the superoxide dismutase in the biological degermation agent bio-enzyme of application example I-10 are more appropriate, the production performance and the immunity of the sow are improved, and the mycotoxin can be changed into nontoxic substances by oxidizing the mycotoxin with hydrogen peroxide, meanwhile, the hydrogen peroxide is further converted into nontoxic water, so that immune organs such as liver, kidney and the like of an animal body can be effectively protected and repaired, and superoxide dismutase can specifically remove harmful free radicals in the body, so that body damage caused by oxidizing certain components in the body by the free radicals can be relieved, and the immunity of the animal body is improved.
In application examples I-12 to I-16, the average weight gain and daily average feed intake of the breeding sows in application example I-15 are respectively 0.28kg and 0.66kg, which are higher than those in application examples I-12 to I-14 and I-16, the feed conversion ratio is 2.39, which is lower than those in application examples I-12 to I-14 and I-11, and the albumin and globulin of the breeding sows in application example I-15 are respectively 43.45g/L and 27.40g/L, which are higher than those in application examples I-12 to I-14 and I-16, which indicates that the mixing amount of the silybum marianum extract and the schisandra extract in the biological mildew removal agent plant extract in application example I-15 is more appropriate, the production performance and immunity of the breeding sows are improved, active oxygen can be cleaned up with the silybum marianum extract, and free radicals in animals are important factors for generating liver problems, can block peroxidation of fatty compounds in time, maintain cell membrane fluidity and healthy physiological function of liver cells, enhance resistance of cell membrane to various injury factors, enhance detoxification capability of liver, protect liver function of fructus Schisandrae extract, promote biosynthesis of protein and glycogen of liver cells, and help regeneration and repair of liver cells.
In application examples I-17 to I-21, the average weight gain and daily average feed intake of the breeding sows of the application example I-20 are respectively 0.31kg and 0.71kg which are higher than those of the application examples I-17 to I-19 and the application example I-21, the feed-meat ratio is 2.32 which is lower than those of the application examples I-17 to I-19 and the application example I-21, and the albumin and globulin of the sow bred by the application example I-20 are respectively 43.54g/L and 27.65g/L which are higher than those of the application examples I-17 to I-19 and the application example I-21, which shows that the mixing amount of the esterified glucomannan and the mannanoligosaccharide which are biologically degermed by the application example I-20 is more appropriate, the production performance and the immunity of the bred sow are improved, may have good physicochemical properties of low heat, stability, safety, no toxicity and the like with the esterified glucomannan, and also has the functions of protecting the intestinal tract, improving the immunity and the like. The mannanoligosaccharide can eliminate the harmful effect of toxin on organism by physical adsorption or direct mycotoxin.
In addition, by combining the application of the comparative examples I-1 to I-6 and the index data of the biological mildew removing agent of the application example I-1, the application discovers that the production performance and the immunity of the breeding sows can be improved to different degrees by adding the modified hydrated aluminosilicate, the lactobacillus reuteri, the lithospermum extract and the glucose oxidase into the raw materials of the biological mildew removing agent.
The following is the application of the biological mold removal agent in the broiler chicken
Application example II-1
The biological mold removal agent obtained in example 1 was added to the feed of broiler chickens at an amount of 1.5 kg/ton from the age of broiler chickens of 70 weeks to the age of broiler chickens of 74 weeks.
Application examples II-2 to II-21
The application examples II-2 to II-21 are the same as the application example II-1 in the application method except that the biological mold remover obtained in example 2-21 is used.
Comparative examples II-1 to II-6
The application comparative examples II-1 to II-6 are the same as the application example II-1 except that the biological mold remover obtained in the comparative examples 1 to 6 is used as the biological mold remover.
Performance detection
The following detection standards or methods are adopted to respectively carry out performance detection on different application examples II-1 to II-21 and application comparative examples II-1 to II-6, and the detection results are shown in Table 7 in detail.
The method comprises the steps of selecting 270 broilers, averagely dividing the broilers into 27 groups, feeding the broilers in 10 groups and 27 groups respectively to feeds obtained in application examples I-1 to I-21 and application comparative examples I-1 to I-6, continuously feeding the broilers for 28 days under the same feeding condition, detecting the initial weight and the tested weight of each group of broilers, calculating daily average weight gain, daily average feed intake and feed-meat ratio, then drawing blood from the 27 groups of broilers after the test, detecting the antibody level of Newcastle disease, namely determining the antibody titer of Newcastle disease, wherein the specific detection result is shown in Table 7.
TABLE 7 measurement results of the Properties of different biological mildew-removing agents
The detection results in table 7 show that the daily average gain and daily average feed intake of the broiler fed with the biological mold removal agent feed obtained by the application are 60.90g and 88.31g respectively, the feed-to-meat ratio is 1.45 at the lowest, and the newcastle disease antibody titer of the broiler is 10.48 at the highest, which shows that the production performance of the broiler fed with the biological mold removal agent feed obtained by the application can be improved, and the immunity of the broiler is improved.
In application examples II-1 to II-3, the daily average feed intake of the application example II-2 broiler chickens is 77.62g, is higher than that of the application example II-1 and the application example II-3, the feed-to-meat ratio is 1.49, is lower than that of the application example II-1 and the application example II-3, the Newcastle disease antibody titer of the application example II-2 broiler chickens is 9.88, and is higher than that of the application example II-1 and the application example II-3, which indicates that the mixing amount of the modified hydrated aluminum silicate in the biological mildew remover of the application example II-2 is proper, the production performance and the immunity of the broiler chickens are improved, and the modified hydrated aluminum silicate by cetyl pyridinium chloride and cetyl trimethyl ammonium bromide is possible to change the pore diameter of the hydrated aluminum silicate, so that the hydrated aluminum silicate has the directional adsorption capacity, the biological mildew remover only adsorbs mycotoxin and does not adsorb nutrient substances, can avoid the situation that partial nutrition is lost due to the addition of the biological mildew removing agent in the feed.
In application examples II-4-II-6, the daily average weight gain and daily average feed intake of the application example II-5 broiler chickens are 53.78g and 79.59g respectively, are higher than the application example II-4 and the application example II-6, the feed-to-meat ratio is 1.48 and are lower than the application example II-4 and the application example II-6, the Newcastle disease antibody titer of the application example II-5 broiler chickens is 9.98 and is higher than the application example II-4 and the application example II-6, and the results show that the mixing amount of lactic acid bacteria in the active beneficial bacteria of the biological mold removal agent of the application example II-5 is proper, the production performance and the immunity of the broiler chickens are improved, specific binding sites with aflatoxin and vomitoxin can be formed between the cell walls of the broiler chickens, the specific binding of the mycotoxin on an intestinal wall inner membrane receptor can be reduced, and the gastrointestinal discomfort caused by the mycotoxin in animal organisms can be reduced; in addition, the toxin is brought out of the animal body through the action of the specific binding site of the cell wall of the lactobacillus and the mycotoxin, and the correlation between the aflatoxin and the vomitoxin can be better removed.
In application examples II-7 to II-11, the daily average weight gain and the daily average feed intake of the broilers in application example II-10 are 56.33g and 82.81g respectively, which are higher than those in application examples II-7 to II-9 and application example II-11, the feed-to-meat ratio is 1.47, which is lower than that in application examples II-7 to II-9 and application example II-11, and the Newcastle disease antibody titer of the broilers in application example II-10 is 10.15, which is higher than that in application examples II-7 to II-9 and application example II-11, which shows that the mixing amount of catalase and superoxide dismutase in the biological mold remover biological enzyme in application example II-10 is more appropriate, the production performance and immunity of the broilers are improved, the mycotoxin is probably utilized with the catalase to oxidize the mycotoxin with the hydrogen peroxide, the mycotoxin is changed into nontoxic substances, and the hydrogen peroxide is further changed into nontoxic water, the product can effectively protect and repair immune organs such as liver and kidney of animal body, and superoxide dismutase can specifically remove harmful free radicals in vivo, so as to relieve body damage caused by oxidizing certain components in vivo by free radicals, and improve immunity of animal body.
In application examples II-12 to II-16, the daily average weight gain and daily average feed intake of application example II-15 broiler chickens are 58.42g and 85.30g respectively, which are higher than those of application examples II-12 to II-14 and application example II-16, the feed-meat ratio is 1.46, which are lower than those of application examples II-12 to II-14 and application example II-11, and the Newcastle antibody titer of application example II-15 broiler chickens is 10.35, which is higher than that of application examples II-12 to II-14 and application example II-16, which indicates that the mixing amount of the silybum marianum extract and the schisandra extract in the biological mold remover plant extract in application example II-15 is more appropriate, the production performance and immunity of the broiler chickens are improved, active oxygen can be cleaned with the silybum marianum extract, free radicals in animals are important factors causing liver problems, and the process of peroxidation of fatty compounds can be blocked in time, maintaining the cell membrane fluidity and healthy physiological function of liver cells, enhancing the resistance of cell membranes to various injury factors, enhancing the detoxification capability of liver, protecting liver function of the schisandra extract, promoting the biosynthesis of protein and glycogen of liver cells, and helping the regeneration and repair of liver cells.
In application examples II-17-II-21, the daily average weight gain and daily average feed intake of the application example II-20 broiler chickens are 60.90g and 88.31g respectively, which are higher than those of the application examples II-17-II-19 and II-21, the feed-meat ratio is 1.45, which is lower than those of the application examples II-17-II-19 and II-21, and the Newcastle antibody titer of the application example II-20 broiler chickens is 10.48, which is higher than those of the application examples II-17-II-19 and II-21, which indicates that the esterified glucomannan and the mannooligosaccharides biologically demoulded in the application example II-20 are appropriate in amount, the production performance and the immunity of the broiler chickens are improved, and the esterified glucomannan have good physicochemical properties of low heat, stability, safety, no toxicity and the like, and the effects of protecting intestinal tracts and improving the immunity and the like. The mannanoligosaccharide can eliminate the harmful effect of toxin on organism by physical adsorption or direct mycotoxin.
In addition, by combining various index data of the application comparative examples II-1 to II-6 and the application example II-1 biological mold removal agent, the application discovers that the productivity and the immunity of the broiler chicken can be improved to different degrees by adding the modified hydrated aluminosilicate, the lactobacillus reuteri, the lithospermum extract and the glucose oxidase into the raw materials of the biological mold removal agent.
The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.
Claims (9)
1. The biological mildew removing agent is characterized by comprising the following raw materials in parts by weight: 70-90 parts of active beneficial bacteria, 10-30 parts of yeast cell walls, 270 parts of modified hydrated aluminosilicate 250-290 parts, 290 parts of plant extracts and 370 parts of biological enzymes 350-370 parts; the modified hydrated aluminosilicate is obtained by modifying cetyl pyridinium chloride and cetyl trimethyl ammonium bromide; the active beneficial bacteria comprise lactobacillus reuteri; the plant extract comprises radix Arnebiae extract; the biological enzyme comprises glucose oxidase.
2. The biological mold remover according to claim 1, characterized by comprising the following raw materials in parts by weight: 75-85 parts of active beneficial bacteria, 15-25 parts of yeast cell wall, 255-265 parts of hydrated aluminosilicate, 285 parts of plant extract and 355-365 parts of biological enzyme.
3. The biological mold remover according to claim 1, wherein the modified hydrated aluminosilicate is prepared by the following steps: slowly adding hydrated aluminum silicate salt into deionized water, uniformly stirring, and mixing the mixture according to the volume ratio of the total dosage of cetylpyridinium chloride and cetyltrimethylammonium bromide to the hydrated aluminum silicate salt of 1: (25-50) adding cetyl pyridinium chloride and cetyl trimethyl ammonium bromide according to the proportion, stirring, shearing, drying, and carrying out superfine grinding to obtain modified hydrated aluminum silicate; the weight ratio of the cetyl pyridinium chloride to the cetyl trimethyl ammonium bromide is 1: (1-3).
4. The biological mold removing agent of claim 1, wherein the active beneficial bacteria comprise the following raw materials in percentage by weight: 20-30% of lactobacillus reuteri, 30-50% of bacillus licheniformis, 20-30% of bacillus subtilis, 1-3% of lactobacillus rhamnosus and 5-7% of lactic acid bacteria.
5. The biological mold remover according to claim 1, wherein the biological enzyme comprises the following raw materials in percentage by weight: 35-40% of glucose oxidase, 20-30% of phytase, 10-25% of catalase and 12-27% of superoxide dismutase.
6. The biological mold remover according to claim 1, wherein the plant extract further comprises the following raw materials in percentage by weight: 30-50% of lithospermum extract, 10-20% of silybum marianum extract, 15-30% of schisandra extract and 20-30% of seaweed extract.
7. The biological mold remover according to claim 1, wherein the biological mold remover further comprises 0.5-1 parts by weight of esterified glucomannan and 15-30 parts by weight of mannooligosaccharides.
8. A process for the preparation of the biological mildew removing agent of any one of claims 1 to 7, which comprises the following steps:
preparing modified hydrated aluminosilicate for later use;
under the aseptic condition, uniformly mixing the raw materials of the biological mildew removing agent to obtain a mixture A;
mixing the mixture A with deionized water in a volume ratio of 1: and (3) uniformly mixing the components in the proportion of (3-5), drying and crushing to obtain the biological mildew removing agent.
9. Use of the biological mold release agent of any one of claims 1 to 7 in animal feed.
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| CN115989848A (en) * | 2022-12-13 | 2023-04-21 | 广东智威农业科技股份有限公司 | Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens |
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| CN115989848A (en) * | 2022-12-13 | 2023-04-21 | 广东智威农业科技股份有限公司 | Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens |
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Denomination of invention: A biological antifungal agent and its preparation method and application Granted publication date: 20240116 Pledgee: China Postal Savings Bank Co.,Ltd. Luannan County Branch Pledgor: Tangshan Zhongxin Feed Co.,Ltd. Registration number: Y2024980025622 |