CN115989848A - Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens - Google Patents

Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens Download PDF

Info

Publication number
CN115989848A
CN115989848A CN202211604258.3A CN202211604258A CN115989848A CN 115989848 A CN115989848 A CN 115989848A CN 202211604258 A CN202211604258 A CN 202211604258A CN 115989848 A CN115989848 A CN 115989848A
Authority
CN
China
Prior art keywords
content
vitamin
feed
broiler chickens
enzyme composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211604258.3A
Other languages
Chinese (zh)
Inventor
瞿浩
付少兵
徐进
刘苑青
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Wiz Agricultural Science & Technology Co ltd
Institute of Animal Science of Guangdong Academy of Agricultural Sciences
Original Assignee
Guangdong Wiz Agricultural Science & Technology Co ltd
Institute of Animal Science of Guangdong Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong Wiz Agricultural Science & Technology Co ltd, Institute of Animal Science of Guangdong Academy of Agricultural Sciences filed Critical Guangdong Wiz Agricultural Science & Technology Co ltd
Priority to CN202211604258.3A priority Critical patent/CN115989848A/en
Publication of CN115989848A publication Critical patent/CN115989848A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention belongs to the technical field of broiler feed, and particularly relates to application of an enzyme composition to preparation of feed for preventing mycotoxin poisoning of broiler chickens. The components of the enzyme composition include beta-D-glucose oxidase and catalase; the enzyme composition accounts for 0.1 to 0.6 percent of the feed consumption; the use mass ratio of the beta-D-glucose oxidase to the catalase in the enzyme composition is 1 (1.5-2), and the enzyme activity of the beta-D-glucose oxidase is more than or equal to 15U/g; the enzyme activity of the catalase is more than or equal to 50U/g. Compared with the prior art, the feed provided by the invention can prevent mycotoxin poisoning of broiler chickens by improving the intestinal barrier function, regulating the cecum microorganism system, improving the antioxidant capacity and enhancing the immunity, and finally has a certain improvement effect on the growth performances such as daily gain, feed-weight ratio and the like of the broiler chickens.

Description

Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens
Technical Field
The invention belongs to the technical field of broiler feed, and particularly relates to application of an enzyme composition to preparation of feed for preventing mycotoxin poisoning of broiler chickens.
Background
Redox balance exists in animals, but when the balance is broken, oxidative stress is induced, thereby endangering healthy growth of animals. In the production of broiler chickens, the pollution of mycotoxin in feed is one of the main factors causing oxidative stress, because feed and raw materials (such as corn) are easily polluted by various moulds in the processes of production, transportation, processing, storage, feeding and the like, and meanwhile, the broiler chickens are easy to ingest the feed polluted by the moulds because a breeding user is difficult to pay attention to the condition of mould development of the feed at any time, and as a result, toxic symptoms such as reduced feeding, emaciation, myogenic gastritis, oxidative stress and even death and the like of the broiler chickens are often caused, and huge economic loss is caused for breeding production.
Therefore, in order to solve the above technical problems, it is needed to provide a feed capable of preventing mycotoxin poisoning of broiler chickens. In the prior art, common mildew removing methods comprise physical methods such as adsorbents, and have certain effects on a certain mycotoxin, but most of the feeds are polluted by various mildew; chemical methods such as ozone, ammonia or alkali addition tend to cause damage to feed nutrients and are poorly palatable; microbial processes are not widely used because of industrial technology limitations and not affordable prices. In the broiler chicken breeding production, glucose oxidase can be used as a mildew inhibitor, has good mildew prevention effect, does not influence palatability and nutrient absorption, but because hydrogen peroxide generated by catalytic reaction belongs to active oxygen, excessive hydrogen peroxide can cause oxidation loss to organisms, and meanwhile, the catalytic reaction has an inhibition effect, so that the effect is reduced; also, a fermentation culture containing the saccharomyces cerevisiae yk18 is adopted, wherein the fermentation culture comprises a saccharomyces cerevisiae yk18 viable bacterium, a saccharomyces cerevisiae cell wall and a saccharomyces cerevisiae fermentation culture medium, and the viable bacterium and the culture are cultivated at relatively high cost; the mycotoxin content of the feed and the excrement is only measured, and although the mycotoxin degradation rate is certainly improved, the effect of the production of the broiler chickens is not compared, the mycotoxin poisoning of the broiler chickens can not be prevented from being reflected in actual production, and the reason for degrading the mycotoxin is unknown.
Disclosure of Invention
In view of the above problems, the present invention aims to provide an enzyme composition for preparing feed for preventing mycotoxin poisoning of broiler chickens.
The technical content of the invention is as follows:
the invention provides an enzyme composition applied to preparing feed for preventing mycotoxin poisoning of broiler chickens, wherein the components of the enzyme composition comprise beta-D-glucose oxidase and catalase;
the enzyme composition accounts for 0.1 to 0.6 percent of the feed consumption;
the use mass ratio of the beta-D-glucose oxidase to the catalase in the enzyme composition is 1 (1.5-2), and the synergistic effect of the beta-D-glucose oxidase and the catalase is as follows: in the process of oxidizing glucose by using oxygen catalyzed by glucose oxidase, the byproduct hydrogen peroxide generated together with the required product gluconic acid can inhibit the catalytic activity of the glucose oxidase, and the hydrogen peroxide can catalyze the decomposition of the hydrogen peroxide to generate oxygen and water, so that the inhibition of the hydrogen peroxide on the catalytic activity of the glucose oxidase is relieved, and the generated oxygen and water promote the substrate concentration of the catalytic reaction of the glucose oxidase;
the enzyme activity of the beta-D-glucose oxidase is more than or equal to 15U/g;
the enzyme activity of the catalase is more than or equal to 50U/g.
The invention also provides a feed for preventing mycotoxin poisoning of broiler chickens, which comprises the components of corn, soybean meal, soybean oil, calcium hydrophosphate type I, stone powder, trace element premix, vitamin premix, L-lysine sulfate, DL-methionine, choline chloride, sodium chloride, betaine and the enzyme composition;
according to weight portions, in the feed, 600 to 650 portions of corn, 290 to 335 portions of bean pulp, 18 to 35 portions of soybean oil, 12 to 20 portions of calcium hydrophosphate I type, 8 to 11 portions of stone powder, 2 portions of microelement premix, 4 portions of vitamin premix, 0.1 to 2.5 portions of L-lysine sulfate, 1 to 3 portions of DL-methionine, 0.5 to 1 portion of choline chloride, 2 to 3 portions of sodium chloride, 0 to 0.2 portion of betaine and 1 to 6 portions of the enzyme composition are calculated;
in the trace element premix, the iron content is 84-180 mg/kg, the copper content is 8.4-18 mg/kg, the zinc content is 56-120 mg/kg and the manganese content is 70-150 mg/kg;
in the vitamin premix, the content of vitamin A is 1900-2000 IU/kg, the content of vitamin D3 is 750-800 IU/kg, the content of vitamin E is 7-8 IU/kg, the content of vitamin K3 is 0.4-0.6 mg/kg, the content of vitamin B1 is 0.4-0.6 mg/kg, the content of vitamin B2 is 1.5-2 mg/kg, the content of vitamin B7 is 1-1.5 mg/kg, and the content of nicotinamide is 9-9.5 mg/kg;
further, the vitamin A content is 1925IU/kg and vitamin D 3 The content of vitamin E is 770IU/kg, the content of vitamin E is 7.78IU/kg, and the content of vitamin K is 770IU/kg 3 The content of vitamin B is 0.52mg/kg 1 The content of vitamin B is 0.52mg/kg 2 The content of vitamin B is 1.74mg/kg 7 The content of nicotinamide is 9.32mg/kg and 1.08 mg/kg.
The beneficial effects of the invention are as follows:
the enzyme composition is used for preparing feeds for preventing mycotoxin poisoning of broiler chickens, the enzyme composition is a mixture of glucose oxidase and catalase, a preliminary mechanism for removing mycotoxin is provided, the enzyme composition is used as a mildew inhibitor, mycotoxin poisoning of broiler chickens can be prevented, the two have a synergistic effect in the enzyme catalytic reaction process, the catalase is added on the basis of the glucose oxidase, the synergistic effect exists in the aspects of promoting animal growth performance, intestinal health, immunity and the like, and meanwhile, the oxidation stress brought by mycotoxin is resisted by the role of an antioxidant; the catalase catalyzes the decomposition of hydrogen peroxide, so that the catalytic reaction of glucose oxidase can be promoted and stabilized, and the glucose oxidase can exert better catalytic effect;
compared with the prior art, the feed provided by the invention can obviously improve the growth performance of broiler chickens, reduce the severity of mycotic myogastric inflammation of broiler chickens, improve the villus morphology and structure in duodenum, jejunum and ileum, obviously improve the gene expression of the intestinal mucosa tight junction protein of broiler chickens, obviously change the composition and structure of the cecum microbiota of broiler chickens fed with moldy corn, maintain the balance of intestinal microbiota by reducing harmful bacteria reproduction and increasing beneficial bacteria, reduce lesions of the liver of broiler chickens, obviously improve the antioxidant capacity to prevent mycotoxin poisoning of broiler chickens and reduce the inflammation level in broiler chickens. In a word, the invention prevents mycotoxin poisoning of broiler chickens by improving the intestinal barrier function, regulating the cecum microbiota, improving the antioxidant capacity and enhancing the immunity, and finally has a certain improvement effect on the daily gain, the feed-to-weight ratio and other growth performances of the broiler chickens.
Drawings
FIG. 1 is a graph of myopic pathology score results;
FIG. 2 shows the morphology and structure of the villi in the duodenum, jejunum and ileum of broiler chickens after feeding;
FIG. 3 is a PCoA graph evaluating beta diversity of cecal microorganisms in broilers;
fig. 4 is a morphology of liver tissue of fed broiler chickens;
fig. 5 is a graph showing the results of lesion numbers and areas in liver tissues of fed broiler chickens.
Detailed Description
The invention is described in further detail below with reference to specific embodiments and the accompanying drawings, it being understood that these embodiments are only for the purpose of illustrating the invention and not for the purpose of limiting the same, and that various modifications of the invention, which are equivalent to those skilled in the art, will fall within the scope of the appended claims after reading the present invention.
All materials and reagents of the invention are materials and reagents of the conventional market unless specified otherwise.
Example 1
An enzyme composition for use in preparing a feed for preventing mycotoxin poisoning of broiler chickens, the feed being shown in table 1, wherein the components of the enzyme composition comprise a mixture of beta-D-glucose oxidase and catalase in a mass ratio of 1:2;
the enzyme activity of the beta-D-glucose oxidase is more than or equal to 15U/g;
the enzyme activity of the catalase is more than or equal to 50U/g.
Table 1 feed Components and nutrient levels (parts by weight)
Figure BDA0003996781400000051
Figure BDA0003996781400000061
In the trace element premix, the iron content is 84-180 mg/kg, the copper content is 8.4-18 mg/kg, the zinc content is 56-120 mg/kg and the manganese content is 70-150 mg/kg;
the vitamin premix contains 1925IU/kg of vitamin A and 1925IU/kg of vitamin D 3 The content of vitamin E is 770IU/kg, the content of vitamin E is 7.78IU/kg, and the content of vitamin K is 770IU/kg 3 The content of vitamin B is 0.52mg/kg 1 The content of vitamin B is 0.52mg/kg 2 The content of vitamin B is 1.74mg/kg 7 The content of nicotinamide is 9.32mg/kg and 1.08 mg/kg.
Example 2
An enzyme composition for use in preparing a feed for preventing mycotoxin poisoning of broiler chickens, the feed being shown in table 2, wherein the components of the enzyme composition comprise a mixture of beta-D-glucose oxidase and catalase in a mass ratio of 1:1.5.
TABLE 2 feed Components and nutrient levels (parts by weight)
Figure BDA0003996781400000062
Figure BDA0003996781400000071
The trace element premix is the same as in example 1;
in the vitamin premix, the vitamin A content is 1900IU/kg, the vitamin D3 content is 750IU/kg, the vitamin E content is 7IU/kg, the vitamin K3 content is 0.4mg/kg, the vitamin B1 content is 0.4mg/kg, the vitamin B2 content is 1.5mg/kg, the vitamin B7 content is 1mg/kg and the nicotinamide content is 9mg/kg.
Example 3
An enzyme composition for use in preparing a feed for preventing mycotoxin poisoning of broiler chickens, the feed being shown in table 3, wherein the components of the enzyme composition comprise a mixture of beta-D-glucose oxidase and catalase in a mass ratio of 1:1.8.
TABLE 3 feed Components and nutrient levels (parts by weight)
Figure BDA0003996781400000072
Figure BDA0003996781400000081
The trace element premix is the same as in example 1;
in the vitamin premix, the content of vitamin A is 2000IU/kg, the content of vitamin D3 is 800IU/kg, the content of vitamin E is 8IU/kg, the content of vitamin K3 is 0.6mg/kg, the content of vitamin B1 is 0.6mg/kg, the content of vitamin B2 is 2mg/kg, the content of vitamin B7 is 1.5mg/kg, and the content of nicotinamide is 9.5mg/kg.
The amount of the enzyme composition to be used may be appropriately adjusted depending on the degree of mycotoxin contamination or the degree of poisoning of broilers.
Test examples
1. Test animals and groups
1 day old Tuhuang No. 2 chicks (spotted-brown chickens) 900 feathers were selected for the trial, setting 3 treatment groups:
CON group: feeding basic diet consisting of normal corn;
MC group: feeding a basal diet consisting of mildewed corn;
MCG group: feeding a basal diet consisting of moldy corn (i.e., the diet of example 1) with the enzyme composition added;
each treatment group was set with 6 replicates, each replicate was 50-feather chickens, different broiler feeding stages (1-21 days and 22-42 days), and the mycotoxin levels obtained in corn and feed are shown in Table 4.
TABLE 4 mycotoxin (μg/kg) content in corn and feed at different feeding stages
Figure BDA0003996781400000091
Wherein AFB1 is aflatoxin B1, DON is vomitoxin, and ZEN is zearalenone.
Comparing the influence of the growth performance of the broiler chickens in different enzyme treatment groups, setting a blank control group, an MC group, an MCG group and a control group 1 (glucose peroxidase+carrier basic diet consisting of moldy corn), a control group 2 (glucose peroxidase+corn starch basic diet consisting of moldy corn), selecting a 1-day old Tuhuang No. 2 chick (ephedra chicken) 1200 feather for test, and setting 6 repeated groups for each treatment group, wherein 50 feather chickens are repeated for each treatment group, and the different broiler chicken raising stages (1-21 days and 22-42 days).
The effect of the different treatment groups on the growth performance of the chickens was recorded and the results are shown in table 5.
TABLE 5 influence of different treatments of group feeds on the growth performance of broilers
Figure BDA0003996781400000092
Figure BDA0003996781400000101
As can be seen from Table 5, the daily gain of the feed of example 1 of the present invention was the highest (except 1-21 d) and the feed weight ratio (daily average feed intake/daily gain) and daily average feed intake were the lowest, indicating that the effect of adding glucose oxidase and catalase simultaneously was superior to that of adding glucose oxidase alone.
2. Test procedure
Before the test, formaldehyde fumigation and flame disinfection are carried out on the henhouse. Adopting a ground flat raising mode, free feeding, and free drinking raising mode; illuminating for 24h at 0-7d, illuminating for 22h at 8-42d, and stopping for 2h; vaccine reference beam-flat immunization program during the test; the whole feeding trial was continued for 42 days and the weight of the broiler chickens on days 1, 21 and 42 and weekly feed intake were recorded.
3. Slaughter test
At the time of raising the broiler chickens to 42 days of age, 2 chickens were randomly extracted in each repetition for slaughter, blood sampling and tissue sampling.
1. Dissecting to observe and score myofascial gastric health symptoms
The scoring rules are: score 1, "normal" means no bleeding or ulceration; 2 minutes, "mild" means a small amount of bleeding and ulceration; the score 3, "severe" refers to major bleeding and ulcers.
2. Tissue sampling
2.1 tissue section sampling: collecting intestinal tract samples of about 1cm of duodenum, jejunum and ileum, cleaning chyme in ice PBS buffer solution, and storing in formalin fixed solution; simultaneously collecting liver about 1cm3, and storing in formalin fixed liquid; is used for observing the morphology and pathological changes of intestinal tracts and liver tissues;
tissue section preparation was sent to the company marchans seville;
2.2 intestinal mucosa sampling: taking about 5cm of duodenum, jejunum and mid-ileum of broiler chickens, flushing chyme with precooled normal saline, then slightly scraping intestinal mucosa with a glass slide, filling into a 2mL freezing tube, and rapidly storing in liquid nitrogen; the method is used for measuring the gene expression, the antioxidation index and the immunity index of the intestinal canal compact connecting protein;
2.3 intestinal content sampling: the cecal content was collected, placed in a centrifuge tube, and snap frozen with liquid nitrogen for microbial 16SrDNA sequencing.
4. Index measurement
4.1 measurement of intestinal claudin Gene expression
Collecting samples of mucosa tissue of duodenum, jejunum and ileum of broiler chickens, placing the samples into a centrifuge tube, adding magnetic beads, placing the samples into a refiner for homogenization, centrifuging the samples, taking supernatant for analysis, taking part of the supernatant for total RNA extraction by a Trizol method, carrying out reverse transcription on the extracted RNA by a 1ststrandcDNA synthesis reaction to synthesize cDNA, and finally detecting the expression condition of intestinal canal compact protein genes (claudin-1, occludin and zo-1) by real-time fluorescence quantitative PCR (qPCR);
4.2 antioxidation index detection
Slaughtering and collecting liver and intestinal mucosa tissue samples of broiler chickens, and measuring antioxidant indexes;
randomly selecting 2 chickens for each repetition, homogenizing intestinal mucosa or liver samples, centrifuging, taking supernatant for analysis, measuring Malondialdehyde (MDA) level by a TBA method and total antioxidant capacity (T-AOC) by a colorimetric method, and measuring antioxidant related indexes in the liver by a commercial antioxidant kit (Nanjing institute of biological engineering);
4.3 immune index detection
Serum and intestinal mucosa were collected for measurement of inflammatory factor levels.
Tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) levels were determined by ELISA kit (Shanghai enzyme-exemption).
5. Results
5.1 broiler growth Performance
TABLE 6 influence of the feed of the invention on the growth performance of broilers
Figure BDA0003996781400000121
As can be seen from the table, in the early stage of the test, namely 1-21d, compared with CON group, after the mildewed corn is fed, the daily average feed intake of the broiler chickens is obviously reduced by 2.34 percent (P < 0.05), the weight of the broiler chickens at 21 days is reduced by 1.44 percent, 2.34 percent and 1.62 percent (P > 0.05) respectively, and the feed weight ratio is increased by 1.28 percent (P > 0.05); compared with the MC group, after the MCG group feed is fed, the average weight (21 d) and the daily gain of the broiler chickens are respectively improved by 1.66 percent, 0.06 percent and 1.86 percent (P > 0.05), and the feed weight ratio is reduced by 1.30 percent (P > 0.05);
in the later test stage, namely 22-42d, compared with CON, after the mildewed corn is fed, the weight of the broiler chickens at 42 days is obviously reduced by 3.93 percent (P < 0.05), the daily average feed intake and daily gain are respectively reduced by 1.19 percent and 5.39 percent (P > 0.05), and the feed weight ratio is increased by 4.15 percent (P > 0.05); compared with the MC group, after the MCG group feed is fed, the weight of the broiler at 42 days, the average daily feed intake and the daily gain are respectively improved by 2.43 percent and 3.13 percent (P > 0.05), and the feed weight ratio is reduced by 2.39 percent (P > 0.05);
during the whole test period, namely 1-41d, compared with CON group, the daily gain of broiler chickens is obviously reduced by 4.10 percent (P < 0.05), the daily average feed intake is reduced by 1.49 (P > 0.05), and the feed weight ratio is increased by 2.87 percent (P > 0.05) after the mildewed corns are fed; compared with the MC group, the daily gain of the broiler chickens is increased by 2.54 percent (P > 0.05) after the MCG group feed is fed, and the feed weight ratio is reduced by 2.79 percent (P > 0.05).
5.2 broiler muscle stomach
As shown in fig. 1, a myogenic pathology score was obtained, wherein all myogenic status was represented by the CON group broiler except for one symptom; in comparison to CON group, almost half of the myostomachs of MC group developed bleeding and inflammatory status of focal ulcers (p=0.061) after broiler chickens were fed with mildewed corn; compared to the MC group, the feeding of the MCG group reduced the severity of myogastritis (P > 0.05).
5.3 intestinal morphology of broiler chickens
As shown in fig. 2, the configuration and structure of the villus in the duodenum, jejunum and ileum are shown, the CON group has more villus, is orderly arranged and has longer length, but after the broiler chickens are fed with the moldy corn (MC group), the villus quantity is smaller, the villus has fracture and adhesion phenomena, and after the broiler chickens are fed with the feed (MCG group), the villus configuration can be obviously improved particularly in the duodenum.
TABLE 7 influence of the feed of the invention on the morphology of the intestinal villi of broilers
Figure BDA0003996781400000141
As can be seen from table 7, after broiler chickens were fed with moldy corn, the crypt depth in the duodenum was significantly increased (P < 0.05); the depth of crypt in duodenum was significantly shallower (P < 0.05) after feeding MCG group feed compared to MC group; the pile height and pile height/crypt depth were not significantly different in each test group (P > 0.05); however, after broiler chickens were fed with moldy corn, the fluff height/crypt depth was the lowest in each test group, with lower ratios indicating lower nutrient absorption capacity.
5.4 Gene expression of the intestinal mucosa Tight-junction protein of broiler chickens
TABLE 8 Effect of the feeds of the invention on the expression of the genes for the Tight-junction protein in the intestinal tract of broilers
Figure BDA0003996781400000151
As shown in table 8, the intestinal claudin gene expression was not significantly altered (P > 0.05) after broiler chickens were fed with moldy corn, but relative mRNA expression of Occludin, claudin-1 and zo-1 in the ileum was at the lowest level, compared to CON group; the relative mRNA expression levels of claudin-1 and zo-1 in duodenum, jejunum and ileum were significantly increased (P < 0.05) and the relative mRNA expression level of Occludin in ileum was also significantly increased (P < 0.05) after feeding MCG group feed compared to MC group.
5.5 cecal microflora of broilers
TABLE 9 Effect of the feeds of the invention on the alpha diversity of the cecum microorganisms of broilers
Figure BDA0003996781400000161
Species numbers and shannon index were used to evaluate the cecal microbial α diversity of broilers, as seen from table 9, the differences in species numbers and shannon index observed for the CON and MC groups were not significant (P > 0.05); compared with the MC group, the feeding of the MCG group feed significantly reduces the number of species and shannon index (P < 0.05); the result shows that the alpha diversity of the cecal microbiota of the broiler fed with the mildewed corn can be obviously reduced after the feed provided by the invention;
as can be seen from fig. 3, the principal coordinate analysis (PCoA) was used to evaluate the beta diversity of the cecal microorganisms of the broiler chickens, which showed that the structural composition of the cecal microorganisms of the broiler chickens was significantly different from that of the CON and MC groups (P < 0.05) after feeding MCG group feed;
to determine the differences in cecal microbiota between the various test groups, the microbial structure composition was analyzed at the phylum, genus and species levels, respectively, as shown in table 9.
TABLE 10 Effect of the feeds of the invention on the composition of the cecal microbiota of broilers
Figure BDA0003996781400000162
At the gate level, feeding MCG group feed significantly increased the relative abundance of actinomycetes gate (actionobacteria) (P < 0.05) compared to CON and MC groups; at the genus level, feeding MCG group feed significantly increased the abundance of bifidobacteria (bifidobacteria) and lactobacilli (Lactobacillus) (P < 0.05) and significantly reduced the abundance of clostridium (P < 0.05); at the seed level, feeding MCG group feed significantly reduced the abundance of Escherichia coli (Escherichia coli) and clostridium spirulina (Clostridium spiroforme) (P < 0.05);
in summary, feeding the feed of the present invention significantly changes the composition and structure of the cecal microbiota of the feeding corn broilers and maintains intestinal microbiota balance by reducing harmful bacterial reproduction and increasing beneficial bacteria.
5.6 liver tissue morphology of broiler chickens
As shown in fig. 4, by observation of liver sections, it was found that liver tissue fed with mildewed corn broiler chickens showed many lesions such as increased nuclei of liver cells (arrow in rectangular box in the figure) and inflammatory cell infiltration (black arrow in the figure).
The number and area of lesions (area surrounded by the curve in the figure) were counted simultaneously in fig. 4, and as shown in fig. 5, there was more lesions (P < 0.05) in the liver tissue of broiler fed with MC group feed than in CON group, while feeding MCG group feed reduced the number of lesions (p=0.079).
5.7 in vivo antioxidant index of broiler chickens
TABLE 11 Effect of the feeds of the present invention on antioxidant levels in corn broiler chickens
Figure BDA0003996781400000171
/>
Figure BDA0003996781400000181
As can be seen from table 11, MDA concentrations were higher in the broiler serum (p=0.067) and ileum (p=0.076) fed the MC group feed and T-AOC concentrations were lower in jejunum (p=0.098) compared to the CON group; compared with the MC group, the MCG group feed can obviously reduce the concentration of malondialdehyde in serum and jejunum (P < 0.05) and obviously increase the total antioxidant capacity in serum and duodenum (P < 0.05).
These results show that the feed of the invention can prevent mycotoxin poisoning of broiler chickens by improving the in vivo antioxidant capacity.
5.8 in vivo inflammation levels in broiler chickens
TABLE 12 Effect of the feeds of the invention on the in vivo inflammation levels of broilers
Figure BDA0003996781400000182
/>
Figure BDA0003996781400000191
As can be seen from table 12, the concentration of interleukin-6 in the duodenum of broiler fed with MC group feed was significantly increased (P < 0.05) and the concentration of tumor necrosis factor- α in the jejunum was significantly increased (P < 0.001) compared to CON group; compared with the MC group, the concentration of IL-6 in serum (P < 0.05) and the concentration of TNF-alpha in serum and jejunum (P < 0.05) are obviously reduced by feeding the MCG group feed.
In conclusion, feeding moldy corn can cause mycotoxin poisoning symptoms of broilers, such as myogastritis, impaired intestinal barrier, liver lesions, reduced antioxidant capacity and enhanced inflammatory response, and finally reduced growth performance. The feed provided by the invention can prevent mycotoxin poisoning of broiler chickens by improving the intestinal barrier function, regulating the cecum microbiota, improving the antioxidant capacity and enhancing the immunity, and finally has a certain improvement effect on the growth performances such as daily gain, feed weight ratio and the like of the broiler chickens.

Claims (9)

1. An enzyme composition for preparing feed for preventing mycotoxin poisoning of broiler chickens, which is characterized in that the components of the enzyme composition comprise beta-D-glucose oxidase and catalase.
2. The application of the enzyme composition according to claim 1 in preparing feed for preventing mycotoxin poisoning of broiler chickens, wherein the enzyme composition accounts for 0.1-0.6% of the feed; the use mass ratio of the beta-D-glucose oxidase to the catalase in the enzyme composition is 1: (1.5-2).
3. The use of the enzyme composition according to claim 1 for preparing feed for preventing mycotoxin poisoning of broiler chickens, wherein the enzyme activity of the beta-D-glucose oxidase is not less than 15U/g.
4. The use of the enzyme composition according to claim 1 for preparing feed for preventing mycotoxin poisoning of broiler chickens, wherein the enzyme activity of the catalase is not less than 50U/g.
5. A feed for preventing mycotoxin poisoning of broiler chickens is characterized by comprising corn, soybean meal, soybean oil, calcium hydrophosphate type I, stone powder, trace element premix, vitamin premix, L-lysine sulfate, DL-methionine, choline chloride, sodium chloride, betaine and an enzyme composition.
6. The feed for preventing mycotoxin poisoning of broiler chickens according to claim 5, wherein 600-650 parts by weight of corn, 290-335 parts by weight of soybean meal, 18-35 parts by weight of soybean oil, 12-20 parts by weight of calcium hydrophosphate type I, 8-11 parts by weight of stone powder, 2 parts by weight of trace element premix, 4 parts by weight of vitamin premix, 0.1-2.5 parts by weight of L-lysine sulfate, 1-3 parts by weight of DL-methionine, 0.5-1 part by weight of choline chloride, 2-3 parts by weight of sodium chloride, 0-0.2 part by weight of betaine and 1-6 parts by weight of the enzyme composition.
7. The feed for preventing mycotoxin poisoning of broiler chickens according to claim 5, wherein the trace element premix has an iron content of 84-180 mg/kg, a copper content of 8.4-18 mg/kg, a zinc content of 56-120 mg/kg, and a manganese content of 70-150 mg/kg.
8. The feed for preventing mycotoxin poisoning of broiler chicken according to claim 5, wherein the vitamin A content in the vitamin premix is 1900-2000 IU/kg, and vitamin D 3 The content of the vitamin E is 750-800 IU/kg, the content of the vitamin E is 7-8 IU/kg, and the content of the vitamin K is 3 The content of the vitamin B is 0.4-0.6 mg/kg 1 The content of the vitamin B is 0.4-0.6 mg/kg 2 The content of vitamin B is 1.5-2 mg/kg 7 The content is 1-1.5 mg/kg, and the content of nicotinamide is 9-9.5 mg/kg.
9. Prevention according to claim 5The feed for the mycotoxin poisoning of the broiler chickens is characterized in that the vitamin A content in the vitamin premix is 1925IU/kg and the vitamin D content is 1925IU/kg 3 The content of vitamin E is 770IU/kg, the content of vitamin E is 7.78IU/kg, and the content of vitamin K is 770IU/kg 3 The content of vitamin B is 0.52mg/kg 1 The content of vitamin B is 0.52mg/kg 2 The content of vitamin B is 1.74mg/kg 7 The content of nicotinamide is 9.32mg/kg and 1.08 mg/kg.
CN202211604258.3A 2022-12-13 2022-12-13 Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens Pending CN115989848A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202211604258.3A CN115989848A (en) 2022-12-13 2022-12-13 Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211604258.3A CN115989848A (en) 2022-12-13 2022-12-13 Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens

Publications (1)

Publication Number Publication Date
CN115989848A true CN115989848A (en) 2023-04-21

Family

ID=85994642

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211604258.3A Pending CN115989848A (en) 2022-12-13 2022-12-13 Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens

Country Status (1)

Country Link
CN (1) CN115989848A (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110506851A (en) * 2019-08-13 2019-11-29 安徽快康生物科技有限公司 A kind of feed addictive and feed for mycotoxin in livestock and poultry body of degrading
CN111280341A (en) * 2020-03-25 2020-06-16 武汉新华扬生物股份有限公司 Compound enzyme preparation and compound feed for preventing and treating necrotic enteritis of poultry
CN113749187A (en) * 2021-08-27 2021-12-07 辽宁威兰生物技术有限责任公司 Production method of glucose oxidase feed additive containing catalase
CN114831248A (en) * 2022-05-30 2022-08-02 唐山市众鑫饲料有限公司 Biological mildew removing agent and preparation method and application thereof
CN115039838A (en) * 2022-06-04 2022-09-13 宁夏夏盛实业集团有限公司 Combination and application of feeding complex enzyme containing microbial fermentation lysozyme and penicillium-derived glucose oxidase
CN115039843A (en) * 2022-05-09 2022-09-13 广东省农业科学院动物科学研究所 Heat stress resistant feed for yellow-feather broilers and application thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110506851A (en) * 2019-08-13 2019-11-29 安徽快康生物科技有限公司 A kind of feed addictive and feed for mycotoxin in livestock and poultry body of degrading
CN111280341A (en) * 2020-03-25 2020-06-16 武汉新华扬生物股份有限公司 Compound enzyme preparation and compound feed for preventing and treating necrotic enteritis of poultry
CN113749187A (en) * 2021-08-27 2021-12-07 辽宁威兰生物技术有限责任公司 Production method of glucose oxidase feed additive containing catalase
CN115039843A (en) * 2022-05-09 2022-09-13 广东省农业科学院动物科学研究所 Heat stress resistant feed for yellow-feather broilers and application thereof
CN114831248A (en) * 2022-05-30 2022-08-02 唐山市众鑫饲料有限公司 Biological mildew removing agent and preparation method and application thereof
CN115039838A (en) * 2022-06-04 2022-09-13 宁夏夏盛实业集团有限公司 Combination and application of feeding complex enzyme containing microbial fermentation lysozyme and penicillium-derived glucose oxidase

Similar Documents

Publication Publication Date Title
JP6514291B2 (en) Dietary intervention improves gastrointestinal health, immunity and function
RU2365621C2 (en) Strain megasphaera elsdenii and its application
CN115322932B (en) Lactobacillus plantarum with anti-alcohol and sobering-up capabilities and application thereof
CN113521115A (en) Aflatoxin detoxification composition and preparation method and application thereof
JP2022541528A (en) Composition of the Clostridial Consortium and Methods of Treatment for Obesity, Metabolic Syndrome and Irritable Bowel Disease
EP2166083A1 (en) Novel Lactobacillus paracasei subsp. paracasei SG96, a bacteriostatic composition containing the same and use thereof
CN110628669B (en) Bacillus subtilis LY-5, microbial inoculum and application thereof and product applying same
Acosta et al. The effect of lactose and a prototype Lactobacillus acidophilus fermentation product on digestibility, nitrogen balance, and intestinal function of weaned pigs
Julliand et al. Effect of dehydrated alfalfa on equine gastric and faecal microbial ecosystems
CN111000052A (en) Piglet enzyme bacterium feed additive and application thereof
CN114854623B (en) Lactobacillus delbrueckii subspecies bulgaricus, microbial inoculum containing lactobacillus delbrueckii subspecies bulgaricus and application of lactobacillus delbrueckii subspecies bulgaricus
CN115989848A (en) Application of enzyme composition in preparing feed for preventing mycotoxin poisoning of broiler chickens
KR100769299B1 (en) Lactobacillus fermentum and dairy products and health-promoting food containing the same
Ghosh et al. Feed utilization efficiency and growth performance in rohu, Labeo rohita (Hamilton, 1822), fingerlings fed yeast extract powder supplemented diets
CN112790294A (en) Special vitamin feed additive for aquatic animals and application thereof
Guo et al. Effects of hydrolyzed gallotannin on intestinal physical barrier, immune function, and microbiota structure of yellow-feather broilers
CN116656526B (en) Lactobacillus plantarum JF4 and application thereof in preparation of blood sugar and cholesterol reducing foods and medicines
CN116162557B (en) A strain of Saccharomyces cerevisiae, chinese medicinal microecological preparation for preventing and treating ruminant diarrhea, and its preparation method
CN117562189B (en) Application of vitamin K in preparing feed for regulating intestinal flora of broiler chickens
CN113025508B (en) Saccharomyces boulardii and application thereof in degrading vomitoxin
KR102050019B1 (en) animal feed additive for immune activity and animal feed comprising the same
CN117946939A (en) Lactobacillus plantarum SM2 and application thereof in preparation of cholesterol-lowering and sleep-aiding foods and medicines
CN117946937A (en) Lactobacillus plantarum XY1 and application thereof in preparation of foods and medicines for reducing blood sugar and improving gout
CN117378702A (en) Poultry feed additive and application thereof
CN117431179A (en) Lactobacillus gasseri MY4 and application thereof in preparing food and medicine for regulating intestines and stomach and reducing uric acid

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination