CN111011595A - Preparation method of compound antidote for livestock and poultry - Google Patents
Preparation method of compound antidote for livestock and poultry Download PDFInfo
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- CN111011595A CN111011595A CN201910777652.9A CN201910777652A CN111011595A CN 111011595 A CN111011595 A CN 111011595A CN 201910777652 A CN201910777652 A CN 201910777652A CN 111011595 A CN111011595 A CN 111011595A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
Abstract
The invention relates to a preparation method of a compound antidote for livestock and poultry. The raw materials of the compound antidote comprise 75-125 parts by weight of traditional Chinese medicines, 20-38 parts by weight of yeast extract, 35-55 parts by weight of aluminosilicate, 0.5-2.5 parts by weight of taurine, 0.5-2.5 parts by weight of amino acid and 0.5-2.5 parts by weight of vitamins. The compound antidote disclosed by the invention adopts multiple components for compound compatibility, and can obviously reduce the death rate, improve the organism immunosuppression of livestock and poultry, reduce the occurrence of complications and improve the production performance of poisoned livestock and poultry by carrying out multi-target prevention and treatment on clinical exterior syndrome of mycotoxin poisoning.
Description
(I) technical field
The invention belongs to the technical field of livestock feed detoxification, and particularly relates to a preparation method of a compound antidote for livestock.
(II) background of the invention
Mycotoxins are ubiquitous in livestock and poultry feeds, and many molds have the potential to metabolize and produce large quantities of mycotoxins in various parts of grain growth, harvesting, storage, and feed processing, transportation, and sale. Mycotoxins are extremely harmful to livestock and poultry farming, especially in summer.
Mycotoxin can cause acute or chronic poisoning of livestock and poultry, the main harm of the poisoning is liver and kidney damage and intestinal bleeding, ascites, digestive dysfunction, neurological symptoms, skin lesion and the like appear, a large amount of mycotoxin can cause the acute poisoning of the livestock and poultry to die clinically, the breeding disorder, abortion and growth inhibition of broilers of the sows are caused, the laying rate of laying hens is low, the egg breaking rate is high, the feed-egg ratio is high, the chronic poisoning caused by the mycotoxin generally has no specific poisoning symptom, and simultaneously, the mild or small amount of clinical manifestations are easily ignored by the breeding person after the mycotoxin is poisoned.
Immunosuppression by destruction of the body's immune system by mycotoxins is its most essential hazard, and many mycotoxins can directly destroy or reduce the structure and function of the body's immune system. For example, certain mycotoxins can atrophie the thymus of pigs, inhibit or reduce the activity of lymphocytes and macrophages, inhibit the functions of antibodies and cytoplasmic products and macrophage reactors, cause the humoral immunity and cellular immunity to be disordered, and the like, thereby seriously reducing the immune response function of the antibodies. The mycotoxin can directly influence the palatability of the feed to cause appetite decrease, and can also directly cause nutrient loss in the feed, and simultaneously, the mycotoxin can also reduce the activity of digestive enzymes in the digestive tract so as to interfere the absorption of organisms on the nutrient, thereby reducing the conversion rate of the feed, increasing the consumption of the feed and further causing the reduction of the production benefit of livestock and poultry breeding.
In actual production, mycotoxin pollution is prevented, mycotoxin poisoning of livestock and poultry is prevented and treated, and means such as preventing growth of mold, reducing absorption of mycotoxin, adjusting metabolic function of organisms, improving mycotoxin poisoning symptoms and the like are mainly adopted. The mildew generation can be reduced by adding mildew preventive to reduce the mildew in the feed raw materials, but the mildew preventive has no effect on the generated mycotoxin. Mycotoxin poisoning is generally prevented using physical, chemical, and biological methods for feed materials that have already produced mycotoxins. At present, the most common method is to reduce the absorption amount of mycotoxin in the feed by livestock and poultry organisms through physical adsorption. But the physical adsorption is selective and can not eliminate all kinds of mycotoxins in the feed. The compound antidote is adopted, and the multi-target effect of the antidote has better effect on preventing and treating mycotoxin poisoning.
Disclosure of the invention
The invention aims to provide a preparation method of a compound antidote for livestock and poultry, which treats both symptoms and root causes, has stable curative effect, and is safe and convenient. The compound antidote has unexpected clinical effect on preventing and treating mycotoxin toxicosis of livestock and poultry. The compound compatibility of various components is adopted, and the multi-target prevention and treatment is carried out on clinical exterior syndrome of mycotoxin poisoning, so that the death rate can be obviously reduced, the immune suppression of organisms can be improved, the occurrence of complications can be reduced, and the production performance of poisoned livestock and poultry can be improved.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention provides a compound antidote for livestock and poultry, which is characterized in that raw materials of the compound antidote comprise 75-125 parts by weight of traditional Chinese medicines, 20-38 parts by weight of yeast extract, 35-55 parts by weight of aluminosilicate, 0.5-2.5 parts by weight of taurine, 0.5-2.5 parts by weight of amino acid and 0.5-2.5 parts by weight of vitamin.
Preferably, the compound antidote for livestock and poultry is characterized in that the traditional Chinese medicine is one or more of lophatherum gracile, mung bean, liquorice and silybum marianum;
preferably, the composite antidote for livestock and poultry is characterized in that the aluminosilicate is one or more of montmorillonite, kaolin and attapulgite;
preferably, the compound antidote for livestock and poultry is characterized in that the amino acid is one or more of lysine, threonine and tryptophan;
preferably, the compound antidote for livestock and poultry is characterized in that the vitamin is one or more of vitamin A, vitamin B, vitamin C and vitamin M.
The invention provides a method for preparing the compound antidote for livestock and poultry, which is characterized by comprising the following process steps: firstly, extracting the traditional Chinese medicines to prepare an extract, then carrying out high-temperature high-pressure treatment and secondary superfine grinding on aluminosilicate to prepare high-temperature modified aluminosilicate, then fully mixing taurine, amino acid and vitamin, then fully mixing the traditional Chinese medicine extract, the yeast extract and the high-temperature modified aluminosilicate in sequence, and sieving to obtain the traditional Chinese medicine preparation.
Preferably, the preparation method of the composite antidote for livestock and poultry is characterized in that the preparation method of the traditional Chinese medicine extract comprises the following steps: crushing lophatherum gracile, mung beans, liquorice and silybum marianum, sieving the crushed lophatherum gracile, adding hot water, stirring the mixture into paste, adjusting the pH value to subacidity, adding compound cellulase, uniformly mixing the mixture, controlling the enzymolysis temperature to 40-60 ℃, keeping the temperature for 3-5 hours, adding 60% ethanol which is 10 times of the volume of the medicine paste, uniformly stirring the mixture, standing the mixture for 48 hours, sucking;
preferably, the preparation method of the composite antidote for livestock and poultry is characterized in that the preparation method of the high-temperature modified aluminosilicate comprises the following steps: the montmorillonite, kaolin and attapulgite powder are calcined at the high temperature of 500 ℃, then are rapidly cooled by introducing dry ice, and then are crushed to 1-5 mu m by an ultrafine crusher, thus obtaining the attapulgite modified montmorillonite/attapulgite composite material.
The harm of mycotoxin to livestock and poultry breeding is reflected in multiple aspects, and the effect of solving the mycotoxin problem of livestock and poultry by only one way is not ideal. The compound antidote provided by the invention adopts traditional Chinese medicine extracts for detoxifying, protecting liver and promoting urination to repair liver injury, improve the liver detoxifying function and accelerate the metabolism of mycotoxin in vivo so as to reduce the damage of mycotoxin to livestock and poultry bodies; the yeast extract has the effects of improving the immunity of organisms and adsorbing mycotoxin, can improve the immunosuppression of livestock and poultry caused by mycotoxin poisoning, and reduces the absorption amount of the livestock and poultry organisms on the mycotoxin; the high-temperature modified aluminosilicate has loose texture and large body surface area, can adsorb various mycotoxins, and reduces the absorption amount of livestock organisms on the mycotoxins; the amino acids and vitamins have effects of relieving fever, resisting inflammation, enhancing immunity, protecting liver, removing toxic substance, stimulating appetite, promoting absorption and conversion of nutrient substances, resisting oxidation, eliminating harmful free radicals, and resisting stress, and can be used for improving clinical symptoms of livestock and poultry, such as body temperature rise, anorexia, weak disease resistance, and various inflammations, caused by mycotoxin poisoning. The components of the invention are systematically compatible, so that the invention has obvious synergistic effect, and the obtained composite antidote has unexpected technical effect on preventing and treating mycotoxin poisoning of livestock and poultry.
The compound antidote for livestock and poultry provided by the invention can be used for preventing and treating mycotoxin poisoning of livestock and poultry, and can also be used for preventing and treating poisoning symptoms caused by heavy metals, chemical drugs, phytotoxins, bacterial endotoxins and the like.
(IV) specific embodiment:
the present invention is further illustrated by, but not limited to, the following examples.
Example 1: a preparation method of a compound antidote for livestock and poultry comprises the following steps: a. taking 50g of lophatherum gracile, 25g of mung bean, 25g of liquorice and 25g of silybum marianum, crushing, sieving by a 50-mesh sieve, adding 300ml of hot water, stirring into paste, adding diluted hydrochloric acid to adjust the pH value to 5.5, adding 5g of compound cellulase, uniformly mixing, controlling the enzymolysis temperature to 50 ℃, keeping the temperature for 3.5 hours, then adding 4000ml of 60% ethanol, uniformly stirring, standing for 48 hours, absorbing supernate, recovering ethanol to obtain clear paste, drying in vacuum, and crushing for later use;
b. adding 15g of montmorillonite powder, 10g of kaolin and 15g of attapulgite powder into a high-temperature calcining furnace, calcining for 1 hour at 500 ℃, introducing dry ice, rapidly cooling, and pulverizing to 1-5 μm by using an ultrafine pulverizer for later use;
c. taking 2g of taurine, 0.25g of lysine, 0.25g of threonine, 0.25g of tryptophan, 0.25g of vitamin A, 0.25g of vitamin B and 0.5g of vitamin C, mixing the components for 30 minutes in advance, then fully mixing the traditional Chinese medicine extract, 25g of yeast extract and high-temperature modified aluminosilicate in sequence, and sieving the mixture by a 100-mesh sieve to obtain the first compound antidote.
Example 2: a preparation method of a compound antidote for livestock and poultry comprises the following steps: a. taking 35g of lophatherum gracile, 30g of liquorice and 20g of silybum marianum, crushing, sieving by a 50-mesh sieve, adding 200ml of hot water, stirring into paste, adding dilute hydrochloric acid to adjust the pH value to 5.2, adding 3.2g of compound cellulase, uniformly mixing, controlling the enzymolysis temperature to 50 ℃, keeping the temperature for 4 hours, then adding 2500ml of 60% ethanol, uniformly stirring, standing for 48 hours, sucking supernatant, recovering ethanol to obtain clear paste, drying in vacuum, and crushing for later use;
b. adding 35g of montmorillonite powder and 20g of attapulgite powder into a high-temperature calcining furnace, calcining for 1 hour at 500 ℃, introducing dry ice, rapidly cooling, and pulverizing to 1-5 mu m by using an ultrafine pulverizer for later use;
c. and 2.5g of taurine, 0.5g of lysine, 0.5g of threonine, 1g of tryptophan, 0.5g of vitamin A, 1g of vitamin C and 1g, and 1g of vitamin M are mixed in advance for 30 minutes, then are fully mixed with the traditional Chinese medicine extract, 35g of yeast extract and high-temperature modified aluminosilicate in sequence, and are sieved by a 100-mesh sieve, so that the compound antidote II disclosed by the invention is obtained.
Clinical validation
In order to verify the clinical prevention and treatment effect of the compound antidote on mycotoxin poisoning of livestock and poultry, the compound antidote is adopted to perform colony prevention and treatment on piglets suffering from mycotoxin poisoning in clinical detection, and compared with a conventional mildew removal agent, the change of clinical symptoms and the death rate of the sick piglets are observed.
The contents of aflatoxin and zearalenone detected in the feed of the sick piglets are higher than the specified limit level of the feed of the piglets by more than 5 times, and investigation shows that the sick piglets have the symptoms of depression, high body temperature, rough and disordered fur, vomiting, diarrhea, red swelling of vulva and the like because the feed becomes damp and mildewed in rainy days and cannot be found in time. The sick pig herd is randomly divided into 4 groups, namely one group of the compound antidote, two groups of the compound antidote, a group of montmorillonite + yeast cell wall and a group of licorice extract + vitamin C, the addition amount of the antidote and the group of montmorillonite + yeast cell wall are 2 kg/ton of material, and the pigs are continuously fed for 10 days. The result shows that the mortality rates of the first group and the second group of the compound antidote are respectively 2.4 percent and 2.8 percent, and the effective rates are respectively 94.3 percent and 95.2 percent; the mortality rates of the (montmorillonite + yeast cell wall) group and the (licorice extract + vitamin C) group are respectively 10.6% and 8.5%, and the effective rates are respectively 74.3% and 75.2%. The results show that the clinical effect of the compound antidote is better than that of the drug of the control group.
The compound antidote is used for preventing the problems of high egg breakage rate, low laying rate, low feed-egg ratio and the like caused by mycotoxin accumulation of laying hens. Laying hens of 26 weeks old in a certain laying hen farm in Changsha, Hunan in 2019 and 3-7-20 days, adding the compound antidote into the feed in an amount of 1 kg/ton for the test group, continuously using the feed for 2 weeks, not performing any treatment on the control group, and continuously observing and recording related production performance indexes until the laying hens are 41 weeks old, which are shown in the following table I.
Table-26 to 41 weeks old layer production performance index data
| Index of production Performance | Test group | Control group | Increase and decrease amplitude (%) |
| Material to egg ratio | 2.06 | 2.24 | -8.04 |
| Egg weight (g) | 60.4 | 59.1 | +2.20 |
| Laying rate (%) | 98.3 | 87.7 | +12.1 |
| Death rate (%) | 2.3 | 3.1 | -16.13 |
| Percentage of broken eggs (%) | 0.62 | 0.86 | -27.91 |
The results show that compared with a control group, the addition of the compound antidote can obviously reduce the death rate, the egg breakage rate and the feed-egg ratio of the laying hens and improve the production performance indexes such as the laying rate, the egg weight and the like.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. A preparation method of a compound antidote for livestock and poultry comprises the following raw materials of 75-125 parts by weight of traditional Chinese medicines, 20-38 parts by weight of yeast extract, 35-55 parts by weight of aluminosilicate, 0.5-2.5 parts by weight of taurine, 0.5-2.5 parts by weight of amino acid and 0.5-2.5 parts by weight of vitamin;
the traditional Chinese medicine is one or more of lophatherum gracile, mung bean, liquorice and silybum marianum;
the aluminosilicate is one or more of montmorillonite, kaolin and attapulgite;
the amino acid is one or more of lysine, threonine and tryptophan;
the vitamin is one or more of vitamin A, vitamin B, vitamin C and vitamin M.
2. A method for preparing the composite antidote for livestock and poultry according to claim 1, which comprises the following process steps: firstly, extracting the traditional Chinese medicines to prepare an extract, then carrying out high-temperature high-pressure treatment and secondary superfine grinding on aluminosilicate to prepare high-temperature modified aluminosilicate, then fully mixing taurine, amino acid and vitamin, then fully mixing the traditional Chinese medicine extract, the yeast extract and the high-temperature modified aluminosilicate in sequence, and sieving to obtain the compound antidote.
The preparation method of the traditional Chinese medicine extract comprises the following steps: crushing lophatherum gracile, mung beans, liquorice and silybum marianum, sieving the crushed lophatherum gracile with 50 meshes of sieve, adding hot water, stirring the mixture into paste, adjusting the pH value to subacidity, adding compound cellulase, uniformly mixing the mixture, controlling the enzymolysis temperature to 40-60 ℃, keeping the temperature for 3-5 hours, adding 60% ethanol which is 10 times of the volume;
the preparation method of the high-temperature modified aluminosilicate comprises the following steps: the montmorillonite, the kaolin and the attapulgite powder are calcined at a high temperature of 500 ℃, then are rapidly cooled by introducing dry ice, and then are crushed to 1-5 mu m by an ultrafine crusher.
3. The compound antidote for livestock and poultry according to claims 1-2, not only can be used for preventing and treating mycotoxin poisoning of livestock and poultry, but also can be used for preventing and treating poisoning symptoms caused by heavy metals, chemical drugs, phytotoxins, bacterial endotoxins and the like.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112998135A (en) * | 2021-03-25 | 2021-06-22 | 河南省健达动保有限公司 | High-efficiency compound mildew-removing and toxin-removing preparation |
| CN115428857A (en) * | 2021-06-03 | 2022-12-06 | 菲吉乐科(中国)生物科技有限公司 | Broad-spectrum mildew removing agent and processing technology and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112998135A (en) * | 2021-03-25 | 2021-06-22 | 河南省健达动保有限公司 | High-efficiency compound mildew-removing and toxin-removing preparation |
| CN115428857A (en) * | 2021-06-03 | 2022-12-06 | 菲吉乐科(中国)生物科技有限公司 | Broad-spectrum mildew removing agent and processing technology and application thereof |
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