CN115413752B - Preparation method and application of plant essential oil detoxication agent - Google Patents
Preparation method and application of plant essential oil detoxication agent Download PDFInfo
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- CN115413752B CN115413752B CN202211246419.6A CN202211246419A CN115413752B CN 115413752 B CN115413752 B CN 115413752B CN 202211246419 A CN202211246419 A CN 202211246419A CN 115413752 B CN115413752 B CN 115413752B
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
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Abstract
The invention discloses a preparation method and application of a plant essential oil detoxication agent, belonging to the technical field of feed additives, wherein the preparation method comprises the following steps: preparing a protective agent, preparing an absorption promoting agent, and mixing for the first time and the second time; the preparation of the absorption promoting agent comprises the steps of mixing lecithin, lemon oil and fish oil, and stirring to obtain mixed milk; adding sodium alginate, soybean polypeptide and corn oligopeptide into deionized water, and stirring to obtain a mixed solution; mixing the mixed milk and the mixed solution, and homogenizing under high pressure to obtain a homogenized solution; adding inulin into the homogeneous solution, stirring, and spray drying to obtain absorption promoter; the plant essential oil detoxication agent prepared by the invention has the effects of improving digestion, inhibiting bacteria and resisting bacteria, can efficiently solve the problems of mould and insect damage in the storage of the prior feed and feed raw materials, and can not influence the absorption of nutrient components in the raw materials when in use.
Description
Technical Field
The invention relates to a preparation method and application of a plant essential oil detoxication agent, and belongs to the technical field of feed additives.
Background
According to data, about 25% of grains in the world are affected by mycotoxins each year, the phenomenon is serious especially in areas with higher temperature and humidity, the mycotoxins and the mycotoxins in livestock and poultry feeds become non-negligible problems, the quality and the nutritional ingredients of the feeds are greatly reduced after the feeds are polluted by the mycotoxins, the mycotoxins cause acute or chronic poisoning of animals, the mycotoxins remained in livestock and poultry products threaten the health of human beings through food chains, the generation and the harm of the mycotoxins are correctly recognized, scientific prevention and treatment are carried out, and the method has important significance for guaranteeing the healthy development of the feeds and the cultivation and the self safety of the human beings.
Mycotoxins can be produced either in the crop field, such as fusarium toxins, or during harvesting and storage, such as aflatoxins, etc., and the growth of mold in grains and feeds and the production of toxins are affected by a number of factors, such as climatic conditions, crop variety, susceptibility of the crop, type of mold, damage to insects or chemicals, etc., and subsequent procedures. After animals eat feeds contaminated by mycotoxins, various acute or chronic toxicities often occur, and some diseases occur, mainly liver cancer and kidney, estrogen disorder syndrome, immunosuppression and the like. The production performance is represented by reducing feed intake, reducing nutrient absorption, affecting feed conversion rate, causing diarrhea, reducing immune function and animal reproductive performance, causing great loss, and the residual toxins of animals can also affect human health.
In order to solve the pollution problem of feed and raw material mycotoxins, on one hand, the quality of the feed raw material is controlled, and the occurrence of toxin transformation is prevented; another aspect is the detoxification of feed materials that have been subjected to mycotoxin contamination. However, mycotoxins are produced in various links of growth, processing, transportation and storage of feed raw materials, so that mildew-proof work is difficult to effectively carry out.
The method for reducing or eliminating mould pollution in production practice mainly comprises a physical detoxification method, a water washing method for removing, a heating detoxification method, a radiation method and the like, wherein chemical detoxification methods such as acid, alkali, acetaldehyde, an oxidant, ozone and the like are used for degrading toxins, and biological detoxification methods such as a mode of fermenting by using certain alcohols and microorganisms are adopted. Most of these methods are not practical, cost-effective, efficient, timely and safe to implement.
The main component types of the mycotoxin adsorbent are mineral adsorbents, yeast cell walls, enzyme preparations, active carbon, plants, nutrient methods, high-molecular substances, compounds thereof and the like, and the most commonly used mycotoxin adsorbent mainly comprises aluminosilicate, montmorillonite and bentonite, but has weak adsorbability to zearalenone, fumagillin and ochratoxin, has no selectivity to adsorbability to a large amount of vitamins and trace elements, thereby influencing the absorption of vitamins and trace elements, and also influencing the digestion of animals.
Disclosure of Invention
The invention provides a preparation method and application of a plant essential oil detoxication agent, and the prepared plant essential oil detoxication agent has the effects of improving digestion, inhibiting bacteria and resisting bacteria, can efficiently solve the problems of mould and insect damage in the storage of the existing feed and feed raw materials, and can not influence the absorption of nutrient components in the raw materials when in use.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a preparation method of a plant essential oil detoxication agent comprises the following steps: preparing a protective agent, preparing an absorption promoting agent, and mixing for the first time and the second time;
the preparation of the protective agent comprises the steps of mixing sodium metabisulfite, trehalose, soybean polysaccharide and erythritol, performing ball milling, controlling the ball-material ratio during ball milling to be 8-10:1, controlling the rotation speed to be 280-300rpm, controlling the time to be 35-38min, obtaining modified sodium metabisulfite after ball milling is finished, heating hydrogenated palm oil to 85-88 ℃, standing for 10-12min, adding modified sodium metabisulfite, citric acid and glucomannan into the hydrogenated palm oil, stirring for 20-23min at the stirring speed of 80-90rpm, cooling at the cooling speed of 1-1.3 ℃/min, heating up to 23-24 ℃, stirring for 5-7min at the temperature of 23-24 ℃, heating up at the heating up speed of 1-1.3 ℃/min, cooling down to 60-65 ℃, stirring for 10-12min at the temperature of 60-65 ℃, stopping stirring, cooling down at the cooling down speed of 3-3.5 ℃/min, cooling down to the temperature of 23-25 ℃, and standing for 0-2 min, and obtaining the protective agent after cooling down to 0-20 min;
in the preparation of the protective agent, the weight ratio of sodium metabisulfite, trehalose, soybean polysaccharide and erythritol is 11-13:3-4:5-7:1-1.4;
in the preparation of the protective agent, the weight ratio of the hydrogenated palm oil to the modified sodium metabisulfite to the citric acid to the glucomannan is 50-53:6-8:2-3:1-2.
The preparation of the absorption enhancer comprises the steps of mixing lecithin, lemon oil and fish oil, and stirring at a stirring speed of 150-180rpm for 30-35min at 38-40 ℃ to obtain mixed milk; adding sodium alginate, soybean polypeptide and corn oligopeptide into deionized water, and stirring at 38-40deg.C and 150-180rpm for 30-35min to obtain mixed solution; mixing the mixed milk and the mixed solution, homogenizing under high pressure, controlling the pressure at 80-85MPa for 13-16min, and homogenizing to obtain homogenized solution; adding inulin into the homogeneous solution, stirring at 30-35deg.C and 150-180rpm for 30-35min, spray drying, controlling air inlet temperature of spray drying to 130-135deg.C, air outlet temperature to 65-70deg.C, and feed liquid flow rate to 120-130mL/min, and spray drying to obtain absorbent;
in the preparation of the absorption enhancer, the weight ratio of lecithin to lemon oil to fish oil is 3-4:10-12:17-20;
in the preparation of the absorption promoting agent, the weight ratio of sodium alginate to soybean polypeptide to corn oligopeptide to deionized water is 5-7:3-4:0.3-0.5:42-45;
in the preparation of the absorption enhancer, the weight ratio of the mixed milk to the mixed liquid is 1:3-3.4;
in the preparation of the absorption enhancer, the weight ratio of the homogeneous solution to the inulin is 28-30:1.
Mixing citronella oil, D-limonene, eugenol, eucalyptus essential oil, cinnamaldehyde and litsea cubeba oil, stirring at a stirring speed of 110-130rpm for 30-35min at 30-35 ℃ to obtain a primary mixed solution, placing silicon dioxide in a spraying fluidized bed, controlling the temperature of the spraying fluidized bed to be 50-55 ℃, continuously spraying and dispersing the primary mixed solution on the silicon dioxide by adopting a multi-point spray gun of the spraying fluidized bed, and obtaining a primary mixture after spraying is finished;
in the primary mixing, the weight ratio of citronella oil to D-limonene to eugenol to eucalyptus essential oil to cinnamaldehyde to litsea cubeba oil to silicon dioxide is 1-1.2:1.5-1.7:2-2.3:0.4-0.6:1.6-1.8:4.8-5:18-20.
Mixing montmorillonite, yeast cell wall, dextran, mannooligosaccharide, sodium humate, cortex Betulae Pendulae and protectant, pulverizing to 200-300 mesh to obtain pulverized material, adding absorbent, primary mixture and pulverized material into stirring equipment, stirring at 27-32deg.C at 180-200rpm for 40-45min to obtain primary detoxication agent; performing high-frequency electromagnetic oscillation treatment on the primary detoxication agent, controlling the frequency of the high-frequency electromagnetic oscillation treatment to be 9-11MHz and the maximum current density to be 17-20A/cm 2 The time is 4-6min, and the high-frequency electromagnetic oscillation treatment is finished to obtain the detoxication agent;
in the secondary mixing, the weight ratio of montmorillonite, yeast cell wall, glucan, mannooligosaccharide, sodium humate, white birch bark and protective agent is 28-30:10-12:9-10:8-10:10-12:4-6:5-7;
in the secondary mixing, the weight ratio of the absorption promoter to the primary mixture to the crushed materials is 8-10:40-44:100-105.
The plant essential oil detoxicant prepared according to the preparation method of the plant essential oil detoxicant.
Compared with the prior art, the invention has the beneficial effects that:
(1) The preparation method of the plant essential oil detoxicant can improve digestion, after the plant essential oil detoxicant prepared by the preparation method is continuously fed to healthy sows for 30 days, the average daily gain is 0.63-0.68kg, the average daily feed intake is 1.37-1.43kg, and the feed conversion ratio is 2.10-2.17;
(2) According to the preparation method of the plant essential oil detoxication agent, the prepared plant essential oil detoxication agent has antibacterial effect, and the plant essential oil detoxication agent prepared by the preparation method is subjected to a bacteriostasis circle test, wherein the diameter of an escherichia coli bacteriostasis circle is 13.8-14.2mm, and the diameter of a staphylococcus aureus bacteriostasis circle is 13.1-13.5mm;
(3) The preparation method of the plant essential oil detoxicant can efficiently solve the problems of mould and insect damage in the storage of the prior feed and feed raw materials, and the plant essential oil detoxicant prepared by the preparation method is subjected to adsorption test of aflatoxin B1 and gibberellin, wherein the adsorption rate of the aflatoxin B1 is 85.52-89.41%, and the adsorption rate of the gibberellin is 81.14-85.85%;
(4) The preparation method of the plant essential oil detoxication agent does not influence the absorption of nutrient components in raw materials when in use.
Detailed Description
Specific embodiments of the present invention will now be described in order to provide a clearer understanding of the technical features, objects and effects of the present invention.
Example 1
A preparation method of plant essential oil detoxication agent specifically comprises the following steps:
1. preparing a protective agent: mixing sodium metabisulfite, trehalose, soybean polysaccharide and erythritol, performing ball milling, controlling the ball-material ratio during ball milling to be 8:1, the rotation speed to be 280rpm, and the time to be 35min, obtaining modified sodium metabisulfite after ball milling is finished, heating hydrogenated palm oil to 85 ℃, standing for 10min, adding modified sodium metabisulfite, citric acid and glucomannan into the hydrogenated palm oil, stirring for 20min at a stirring speed of 80rpm, cooling at a cooling speed of 1 ℃/min, stirring for 5min at 23 ℃, heating at a heating speed of 1 ℃/min, heating to 60 ℃, stirring for 10min at 60 ℃, cooling at a cooling speed of 1 ℃/min, cooling to 23 ℃, stirring for 10min at 23 ℃, stopping stirring, cooling at a cooling speed of 3 ℃/min, standing for 18min at 0 ℃ to obtain a protective agent;
wherein the weight ratio of sodium metabisulfite, trehalose, soybean polysaccharide and erythritol is 11:3:5:1;
wherein the weight ratio of the hydrogenated palm oil to the modified sodium metabisulfite to the citric acid to the glucomannan is 50:6:2:1.
2. Preparing an absorption promoting agent: mixing lecithin, lemon oil and fish oil, and stirring at 38deg.C and 150rpm for 30min to obtain mixed milk; adding sodium alginate, soybean polypeptide and corn oligopeptide into deionized water, and stirring at 38deg.C and stirring speed of 150rpm for 30min to obtain mixed solution; mixing the mixed milk and the mixed solution, homogenizing under high pressure, controlling the pressure at 80MPa and the time at 13min, and obtaining homogenized solution after homogenizing; adding inulin into the homogeneous solution, stirring at 30deg.C and 150rpm for 30min, spray drying, controlling air inlet temperature of spray drying to 130deg.C, air outlet temperature to 65deg.C, and feed liquid flow rate to 120mL/min, and spray drying to obtain absorbent;
wherein the weight ratio of lecithin, lemon oil and fish oil is 3:10:17;
wherein the weight ratio of sodium alginate to soybean polypeptide to corn oligopeptide to deionized water is 5:3:0.3:42;
wherein the weight ratio of the mixed milk to the mixed liquid is 1:3;
wherein the weight ratio of the homogeneous solution to the inulin is 28:1.
3. Primary mixing: mixing citronella oil, D-limonene, eugenol, eucalyptus essential oil, cinnamaldehyde and litsea cubeba oil, stirring at a stirring speed of 110rpm for 30min at 30 ℃ to obtain a primary mixed solution, placing silicon dioxide into a spray fluidized bed, controlling the temperature of the spray fluidized bed to be 50 ℃, continuously spraying and dispersing the primary mixed solution onto the silicon dioxide by adopting a multi-point spray gun of the spray fluidized bed, and obtaining a primary mixture after spraying is finished;
wherein, the weight ratio of citronella oil, D-limonene, eugenol, eucalyptus essential oil, cinnamaldehyde, litsea cubeba oil and silicon dioxide is 1:1.5:2:0.4:1.6:4.8:18.
4. Secondary mixing: mixing montmorillonite, yeast cell wall, dextran, mannooligosaccharide, sodium humate, cortex Betulae Pendulae and protectant, pulverizing to 200 mesh to obtain pulverized material, adding absorbent, primary mixture and pulverized material into stirring equipment, stirring at 27deg.C at 180rpm for 40min to obtain primary detoxication agent; performing high-frequency electromagnetic oscillation treatment on the primary detoxication agent, controlling the frequency at 9MHz and the maximum current density at 17A/cm during the high-frequency electromagnetic oscillation treatment 2 The time is 4min, and the high-frequency electromagnetic oscillation treatment is finished to obtain the detoxication agent;
wherein, the weight ratio of montmorillonite, yeast cell wall, glucan, mannooligosaccharide, sodium humate, white birch bark and protective agent is 28:10:9:8:10:4:5;
wherein the weight ratio of the absorption promoter to the primary mixture to the crushed materials is 8:40:100.
Example 2
A preparation method of plant essential oil detoxication agent specifically comprises the following steps:
1. preparing a protective agent: mixing sodium metabisulfite, trehalose, soybean polysaccharide and erythritol, performing ball milling, controlling the ball-material ratio during ball milling to be 9:1, the rotation speed to be 290rpm, and the time to be 36min, obtaining modified sodium metabisulfite after ball milling, heating hydrogenated palm oil to 87 ℃, standing for 11min, adding modified sodium metabisulfite, citric acid and glucomannan into the hydrogenated palm oil, stirring for 21min at a stirring speed of 85rpm, cooling at a cooling speed of 1.2 ℃/min, stirring for 6min at 23.5 ℃, heating at a heating speed of 1.2 ℃/min, stirring for 11min at 62 ℃, cooling at a cooling speed of 1.2 ℃/min, cooling to 24 ℃, stirring for 11min at 24 ℃, stopping stirring, cooling at a cooling speed of 3.2 ℃/min, cooling to 1 ℃, and standing for 19min at 1 ℃ to obtain a protective agent;
wherein the weight ratio of sodium metabisulfite, trehalose, soybean polysaccharide and erythritol is 12:3.5:6:1.2;
wherein the weight ratio of the hydrogenated palm oil to the modified sodium metabisulfite to the citric acid to the glucomannan is 52:7:2.5:1.5.
2. Preparing an absorption promoting agent: mixing lecithin, lemon oil and fish oil, and stirring at 39deg.C and 160rpm for 32min to obtain mixed milk; adding sodium alginate, soybean polypeptide and corn oligopeptide into deionized water, and stirring at 39deg.C and 160rpm for 32min to obtain a mixed solution; mixing the mixed milk and the mixed solution, homogenizing under high pressure, controlling the pressure at 82MPa and the time at 14min, and obtaining homogenized solution after homogenizing; adding inulin into the homogeneous solution, stirring at 32deg.C and 160rpm for 32min, spray drying, controlling air inlet temperature of spray drying to be 132 deg.C, air outlet temperature to be 67 deg.C, and feed liquid flow rate to be 125mL/min, and spray drying to obtain absorbent;
wherein the weight ratio of lecithin, lemon oil and fish oil is 3.5:11:18;
wherein the weight ratio of sodium alginate to soybean polypeptide to corn oligopeptide to deionized water is 6:3.5:0.4:43;
wherein the weight ratio of the mixed milk to the mixed liquid is 1:3.2;
wherein the weight ratio of the homogeneous solution to the inulin is 29:1.
3. Primary mixing: mixing citronella oil, D-limonene, eugenol, eucalyptus essential oil, cinnamaldehyde and litsea cubeba oil, stirring at a stirring speed of 120rpm for 32min at 32 ℃ to obtain a primary mixed solution, placing silicon dioxide into a spray fluidized bed, controlling the temperature of the spray fluidized bed to be 53 ℃, adopting a multipoint spray gun of the spray fluidized bed to continuously spray and disperse the primary mixed solution onto the silicon dioxide, and obtaining a primary mixture after the spraying is finished;
wherein, the weight ratio of citronella oil, D-limonene, eugenol, eucalyptus essential oil, cinnamaldehyde, litsea cubeba oil and silicon dioxide is 1.1:1.6:2.2:0.5:1.7:4.9:19.
4. Secondary mixing: mixing montmorillonite, yeast cell wall, dextran, mannooligosaccharide, sodium humate, cortex Betulae Pendulae, and protectant, pulverizing to 250 mesh, adding absorbent, primary mixture, and pulverized material into stirring equipment, stirring at 30deg.C and 190rpm for 42min to obtain the final productPrimary detoxication; performing high-frequency electromagnetic oscillation treatment on the primary detoxication agent, controlling the frequency of the high-frequency electromagnetic oscillation treatment to be 10MHz and the maximum current density to be 18A/cm 2 The time is 5min, and the high-frequency electromagnetic oscillation treatment is finished to obtain the detoxication agent;
wherein, the weight ratio of montmorillonite, yeast cell wall, glucan, mannooligosaccharide, sodium humate, white birch bark and protective agent is 29:11:9.5:9:11:5:6;
wherein the weight ratio of the absorption promoter to the primary mixture to the crushed materials is 9:42:102.
Example 3
A preparation method of plant essential oil detoxication agent specifically comprises the following steps:
1. preparing a protective agent: mixing sodium metabisulfite, trehalose, soybean polysaccharide and erythritol, performing ball milling, controlling the ball-material ratio during ball milling to be 10:1, the rotating speed to be 300rpm, and the time to be 38min, obtaining modified sodium metabisulfite after ball milling is finished, heating hydrogenated palm oil to 88 ℃, standing for 12min, adding modified sodium metabisulfite, citric acid and glucomannan into the hydrogenated palm oil, stirring for 23min at a stirring speed of 90rpm, cooling at a cooling speed of 1.3 ℃/min, stirring for 7min at 24 ℃, heating at a heating speed of 1.3 ℃/min, heating to 65 ℃, stirring for 12min at 65 ℃, cooling at a cooling speed of 1.3 ℃/min, cooling to 25 ℃, stirring for 12min at 25 ℃, stopping stirring, cooling at a cooling speed of 3.5 ℃/min, and standing for 20min at 2 ℃ to obtain the protective agent;
wherein the weight ratio of sodium metabisulfite, trehalose, soybean polysaccharide and erythritol is 13:4:7:1.4;
wherein the weight ratio of the hydrogenated palm oil to the modified sodium metabisulfite to the citric acid to the glucomannan is 53:8:3:2.
2. Preparing an absorption promoting agent: mixing lecithin, lemon oil and fish oil, and stirring at 40deg.C and 180rpm for 35min to obtain mixed milk; adding sodium alginate, soybean polypeptide and corn oligopeptide into deionized water, and stirring at 40 ℃ for 35min at a stirring speed of 180rpm to obtain a mixed solution; mixing the mixed milk and the mixed solution, homogenizing under high pressure, controlling the pressure at 85MPa and the time at 16min, and obtaining homogenized solution after homogenizing; adding inulin into the homogeneous solution, stirring at 35deg.C and 180rpm for 35min, spray drying, controlling air inlet temperature of spray drying to be 135deg.C, air outlet temperature to be 70deg.C, and feed liquid flow rate to be 130mL/min, and spray drying to obtain absorbent;
wherein the weight ratio of lecithin, lemon oil and fish oil is 4:12:20;
wherein the weight ratio of sodium alginate to soybean polypeptide to corn oligopeptide to deionized water is 7:4:0.5:45;
wherein the weight ratio of the mixed milk to the mixed liquid is 1:3.4;
wherein the weight ratio of the homogeneous solution to the inulin is 30:1.
3. Primary mixing: mixing citronella oil, D-limonene, eugenol, eucalyptus essential oil, cinnamaldehyde and litsea cubeba oil, stirring at 35 ℃ for 35min at a stirring speed of 130rpm to obtain a primary mixed solution, placing silicon dioxide in a spray fluidized bed, controlling the temperature of the spray fluidized bed to be 55 ℃, continuously spraying and dispersing the primary mixed solution on the silicon dioxide by adopting a multi-point spray gun of the spray fluidized bed, and obtaining a primary mixture after spraying is finished;
wherein, the weight ratio of citronella oil, D-limonene, eugenol, eucalyptus essential oil, cinnamaldehyde, litsea cubeba oil and silicon dioxide is 1.2:1.7:2.3:0.6:1.8:5:20.
4. Secondary mixing: mixing montmorillonite, yeast cell wall, dextran, mannooligosaccharide, sodium humate, cortex Betulae Pendulae and protectant, pulverizing to 300 mesh to obtain pulverized material, adding absorbent, primary mixture and pulverized material into stirring equipment, stirring at 32deg.C at 200rpm for 45min to obtain primary detoxication agent; performing high-frequency electromagnetic oscillation treatment on the primary detoxication agent, controlling the frequency at 11MHz and the maximum current density at 20A/cm 2 The time is 6min, and the high-frequency electromagnetic oscillation treatment is finished to obtain the detoxication agent;
wherein, the weight ratio of montmorillonite, yeast cell wall, glucan, mannooligosaccharide, sodium humate, white birch bark and protective agent is 30:12:10:10:12:6:7;
wherein the weight ratio of the absorption promoter to the primary mixture to the crushed materials is 10:44:105.
Comparative example 1
The same method for preparing a plant essential oil antidote as in example 1 is different in that: the step of preparing the protective agent in step 1 is omitted, and the addition of the protective agent is omitted in the step of secondary mixing in step 4.
Comparative example 2
The same method for preparing a plant essential oil antidote as in example 1 is different in that: the step of preparing the absorption enhancer in the step 2 is omitted, and the addition of the absorption enhancer is omitted in the step 4 secondary mixing step.
Test example 1
Selecting 5 healthy Duroc fattening pigs in a Weifang city in Shandong province, wherein the weight is 90-95kg, the feeding period is 6 months, the feed is corn and bean pulp added with premix before slaughtering, pigs are bloodletting slaughtered, the front section and the middle section of small intestine are immediately taken after viscera are separated, small intestine chyme is respectively collected, the duodenum and jejunum chyme are obtained to be 5kg in total, the chyme is quickly transported back to a laboratory, the chyme is respectively centrifuged for 10 minutes at 10000rpm, suspended matters are filtered by 5 layers of gauze, small intestine liquid is obtained, the small intestine liquid is respectively filled into a 100ml small plastic reagent bottle, and the small intestine liquid is frozen for later use and is dissolved for direct use.
0.1g of the plant essential oil detoxication agent prepared in examples 1-3 and comparative examples 1-2 was weighed into 50ml volumetric flasks, and then fixed in volume with gastric juice, and stirred for 30min using a magnetic stirrer to obtain mixed solutions No. 1-5, corresponding relationships as follows:
placing the mixed solution No. 1-5 in a refrigerator at 4 ℃ for 23 hours, taking out the mixed solution No. 1-5 for continuing magnetic stirring after half an hour before adsorption, then taking 200uL of each mixed solution No. 1-5 in a 1.5mL centrifuge tube, adding 100uL of each of an aflatoxin B1 solution and a 40ug/mL zearalenone solution, adsorbing for 8 hours at 1200r/min in a 37 ℃ metal bath, centrifuging for 10 minutes at 10000rpm, taking a supernatant, detecting aflatoxin B1 and gibberellin in the supernatant, calculating the adsorption rate of aflatoxin B1 and gibberellin, and calculating the calculation result as follows:
test example 2
Selecting healthy sows with the age of 9 months and the weight of 90-95kg from a bamboo forest farm in a city of Weifang in Shandong province, dividing 40 healthy sows into 5 groups, numbering 1-5 groups, adding the plant essential oil detoxicant prepared in the examples 1-3 and the comparative examples 1-2 into feeds of 1-5 healthy sows respectively, uniformly stirring, controlling the weight ratio of the plant essential oil detoxicant to the feeds to be 1:50, and feeding the feeds added with the plant essential oil detoxicant to 1-5 healthy sows respectively, wherein the corresponding relation is as follows:
3 times per day, ensuring sufficient feed, cleaning and sterilizing pig house before test, feeding pigs separately, feeding pigs freely, recording average daily gain after 30d of feed addition, average daily feed intake, feed-meat ratio, and recording the following results:
test example 3
Taking 5 parts of 200mL nutrient broth culture medium with the number of 1-5, adding escherichia coli and staphylococcus aureus with the final concentrations of 104cfu/mL and 105cfu/mL into the 1-5 nutrient broth culture medium respectively to prepare a 1-5 sterile flat plate (with the thickness of 5 mm), placing a sterile oxford cup with the diameter of 8mm on the flat plate after the flat plate is solidified, and adding 0.2g of plant essential oil detoxicant prepared in the examples 1-3 and the comparative examples 1-2 into the 1-5 sterile flat plate respectively, wherein the corresponding relations are as follows:
culturing in an incubator at 37 ℃ for 18 hours, and then observing and recording the diameter of the inhibition zone, wherein the recording result is as follows:
the percentages used in the present invention are mass percentages unless otherwise indicated.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (2)
1. The preparation method of the plant essential oil detoxication agent is characterized by comprising the following steps: preparing a protective agent, preparing an absorption promoting agent, and mixing for the first time and the second time;
the preparation of the protective agent comprises the steps of mixing sodium metabisulfite, trehalose, soybean polysaccharide and erythritol, performing ball milling, controlling the ball-material ratio during ball milling to be 8-10:1, controlling the rotation speed to be 280-300rpm, controlling the time to be 35-38min, obtaining modified sodium metabisulfite after ball milling is finished, heating hydrogenated palm oil to 85-88 ℃, standing for 10-12min, adding modified sodium metabisulfite, citric acid and glucomannan into the hydrogenated palm oil, stirring for 20-23min at the stirring speed of 80-90rpm, cooling at the cooling speed of 1-1.3 ℃/min, heating up to 23-24 ℃, stirring for 5-7min at the temperature of 23-24 ℃, heating up at the heating up speed of 1-1.3 ℃/min, cooling down to 60-65 ℃, stirring for 10-12min at the temperature of 60-65 ℃, stopping stirring, cooling down at the cooling down speed of 3-3.5 ℃/min, cooling down to the temperature of 23-25 ℃, and standing for 0-2 min, and obtaining the protective agent after cooling down to 0-20 min;
in the preparation of the protective agent, the weight ratio of sodium metabisulfite, trehalose, soybean polysaccharide and erythritol is 11-13:3-4:5-7:1-1.4;
in the preparation of the protective agent, the weight ratio of the hydrogenated palm oil to the modified sodium metabisulfite to the citric acid to the glucomannan is 50-53:6-8:2-3:1-2;
the preparation of the absorption enhancer comprises the steps of mixing lecithin, lemon oil and fish oil, and stirring at a stirring speed of 150-180rpm for 30-35min at 38-40 ℃ to obtain mixed milk; adding sodium alginate, soybean polypeptide and corn oligopeptide into deionized water, and stirring at 38-40deg.C and 150-180rpm for 30-35min to obtain mixed solution; mixing the mixed milk and the mixed solution, homogenizing under high pressure, controlling the pressure at 80-85MPa for 13-16min, and homogenizing to obtain homogenized solution; adding inulin into the homogeneous solution, stirring at 30-35deg.C and 150-180rpm for 30-35min, spray drying, controlling air inlet temperature of spray drying to 130-135deg.C, air outlet temperature to 65-70deg.C, and feed liquid flow rate to 120-130mL/min, and spray drying to obtain absorbent;
in the preparation of the absorption enhancer, the weight ratio of lecithin to lemon oil to fish oil is 3-4:10-12:17-20;
in the preparation of the absorption promoting agent, the weight ratio of sodium alginate to soybean polypeptide to corn oligopeptide to deionized water is 5-7:3-4:0.3-0.5:42-45;
in the preparation of the absorption enhancer, the weight ratio of the mixed milk to the mixed liquid is 1:3-3.4;
in the preparation of the absorption enhancer, the weight ratio of the homogeneous solution to the inulin is 28-30:1;
mixing citronella oil, D-limonene, eugenol, eucalyptus essential oil, cinnamaldehyde and litsea cubeba oil, stirring at a stirring speed of 110-130rpm for 30-35min at 30-35 ℃ to obtain a primary mixed solution, placing silicon dioxide in a spraying fluidized bed, controlling the temperature of the spraying fluidized bed to be 50-55 ℃, continuously spraying and dispersing the primary mixed solution on the silicon dioxide by adopting a multi-point spray gun of the spraying fluidized bed, and obtaining a primary mixture after spraying is finished;
in the primary mixing, the weight ratio of citronella oil to D-limonene to eugenol to eucalyptus essential oil to cinnamaldehyde to litsea cubeba oil to silicon dioxide is 1-1.2:1.5-1.7:2-2.3:0.4-0.6:1.6-1.8:4.8-5:18-20;
mixing montmorillonite, yeast cell wall, dextran, mannooligosaccharide, sodium humate, cortex Betulae Pendulae and protectant, pulverizing to 200-300 mesh to obtain pulverized material, adding absorbent, primary mixture and pulverized material into stirring equipment, stirring at 27-32deg.C at 180-200rpm for 40-45min to obtain primary detoxication agent; performing high-frequency electromagnetic oscillation treatment on the primary detoxication agent, controlling the frequency of the high-frequency electromagnetic oscillation treatment to be 9-11MHz and the maximum current density to be 17-20A/cm 2 The time is 4-6min, and the high-frequency electromagnetic oscillation treatment is finished to obtain the detoxication agent;
in the secondary mixing, the weight ratio of montmorillonite, yeast cell wall, glucan, mannooligosaccharide, sodium humate, white birch bark and protective agent is 28-30:10-12:9-10:8-10:10-12:4-6:5-7;
in the secondary mixing, the weight ratio of the absorption promoter to the primary mixture to the crushed materials is 8-10:40-44:100-105.
2. A plant essential oil detoxifier prepared according to the method of claim 1.
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