CN109938244A - A kind of high-efficiency feed mold toxin sorbent preparation process - Google Patents
A kind of high-efficiency feed mold toxin sorbent preparation process Download PDFInfo
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Abstract
The invention discloses a kind of high-efficiency feed mold toxin sorbent preparation processes, are made of modified montmorillonite used, yeast extract, lactobacillus acidophilus, Lactobacillus amylophilus, saliva Lactobacillus lactis, streptococcus thermophilus, bacillus subtilis, Honegsukle flower P.E, camellia oil extract, glycyrrhizic acid, Echinacea Purpurea Herb P.E, eugenol, cinnaldehydrum, baicalin, vitamin C, vitamin E, zinc methionine.Mold toxin sorbent provided by the invention has wide spectrum absorption and degradation characteristic to a variety of common mycotoxins; product of the present invention improves the conversion ratio of feed; it can promote animal and bird intestines colony balance simultaneously; the zinc methionine added in formula can also play the role of liver-protective; selected raw material is naturally pollution-free, has good application value and market promotion prospect to feed and cultivation industry.
Description
Technical field
The invention belongs to feed addictive degrading mold toxin technical fields, relate generally to a kind of high-efficiency feed mycotoxin
Adsorbent preparation process.
Background technique
Mycotoxin contamination problem has become one of Feed Enterprise and breeding enterprise facing in feed and raw material
Global problem, mycotoxin are the anti-nutritional factors being present in feed and feedstuff, are by flavatin, Penicillium notatum
Element, the secondary metabolite that generates under certain temperature and humidity condition of fusarine, endangering in production process biggish mainly has: Huang Qu
Mould toxin, Trichothecenes toxin, zearalenone, fumarubicin, ochratoxin etc..Wherein aflatoxin
It is the compound of the strong biology alkalinity of one kind generated by aspergillus flavus and aspergillus parasiticus, and strongest carcinogenicity object so far
Matter, the crop of main harm have the crops such as peanut, rice, beans.
Mycotoxin almost influences each organ of livestock and poultry, especially to liver, kidney, oral cavity, gastrointestinal tract, nerveous system
System, reproductive system directly translate into the decline of livestock and poultry feed intake, the slow, vomiting of weight gain etc., so that feed in appearance
Conversion ratio decline.Zearalenone mainly acts on reproductive system, when zearalenone poisoning directly translates into wig
Feelings, miscarriage, vulva is red and swollen, infertile, lactation is unusual etc..Trichothecenes toxin mainly includes T-2, DON, DAS toxin, these
Stronger alkalinity is presented in toxin, and such toxin poisoning main harm oral cavity, gastrointestinal tract, nervous system, they are by influencing albumen
Matter synthesis and immunity of organism play toxic effect.
As people endanger mycotoxin, understanding is gradually increased and mycotoxin is to the great of China's livestock and poultry cultivation
The existing a large amount of report of harm, more and more researchers remove to seek to develop a kind of reduction toxicity of mycotoxins or to reduce its right
The method of food and contamination of raw material, as that can prevent crop from being infected by mould by preferable precautionary measures before crop harvesting,
Carried out after crop harvesting crop and process finished product storage work (drying, product classification graduation storage, reduce storage temperature,
Humidity) be controlled, however in daily production, mycotoxin contamination problem be it is unavoidable, therefore, seek properly to have
The method of effect carries out processing control to mycotoxin, is the task of top priority of animal husbandry development.
Mainly have to detoxicating mycotoxin processing at present: mechanical detoxification, biological detoxication, chemical detoxication and physical absorption detoxification
The methods of.Wherein, physics detoxification trivial operations, it is with high costs the defects of, chemical detoxication can not be in life because of chemical residues problem
It produces and is effectively used in practice；Adsorbent detoxicity method by adding various adsorbents in feed or cereal, can mainly adsorb
The substance of mycotoxin makes toxin by not being absorbed by animals when animal intestinal tract, is expelled directly out external.This is current feed
More mature in the market and a kind of more feasible detoxicating mycotoxin method, but mycotoxin structure diversity common adsorbents are not
It can effectively solve the pollution and harm problem of a variety of mycotoxins.The mold toxin sorbent studied extensively at present includes clay class
Adsorbent or organic polymer, but effect is undesirable, in view of this, the present invention relates to a kind of novel livestock and poultry to use mould
Toxin degradation feed addictive preparation process.
Summary of the invention
For some problems existing for mould dose of product are taken off at present, the present invention provides a kind of by inhibiting mould to breed, degrade
With the modes such as absorbing mycotoxin, the pollution problem of livestock and poultry cultivation mycotoxins in feed is solved, while animal and bird intestines bacterium can be promoted
Group's balance, improves the mold toxin sorbent and preparation process of the immunocompetence of livestock and poultry.
Mold toxin sorbent provided by the invention, technical solution are as follows:
Mold toxin sorbent provided by the invention is it is characterized in that, by probiotics, vitamin, modified montmorillonite used, yeast cells
The main component of wall, natural plant extracts, minerals composition mold toxin sorbent.
The probiotics by lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus amylophilus
(Lactobacillusamylovorus), saliva Lactobacillus lactis (Lactobacillus salivarius), streptococcus thermophilus
(Streptococcus thermophilus), bacillus subtilis (Bacillus subtilis) composition.
The natural plant extracts be by Honegsukle flower P.E, camellia oil extract, glycyrrhizic acid, Echinacea Purpurea Herb P.E,
Eugenol, cinnaldehydrum, baicalin composition.
The vitamin is made of vitamin C, vitamin E.
The minerals are made of zinc methionine.
A kind of high-efficiency feed mold toxin sorbent preparation process, it is characterised in that: the mycotoxin that the present invention announces is inhaled
Attached dose, modified montmorillonite used mass fraction accounts for 4%-40%, and yeast cell wall mass fraction accounts for 50%-55%, and vitamin mass fraction accounts for
1%-3%, probiotic powder mass fraction account for 1%-2%, natural plant extracts mass fraction accounts for 3%-5%, minerals mass fraction accounts for
5%-7%。
The probiotic powder is according to lactobacillus acidophilus: Lactobacillus amylophilus: saliva Lactobacillus lactis: streptococcus thermophilus: withered grass
Bacillus=4:4:3:3:3 ratio is mixed.
The lactobacillus acidophilus, Lactobacillus amylophilus, saliva Lactobacillus lactis, streptococcus thermophilus are separated pure by animal and bird intestines
Change amplification gained.
The natural plant extracts be by Honegsukle flower P.E, camellia oil extract, glycyrrhizic acid, Echinacea Purpurea Herb P.E,
Eugenol, cinnaldehydrum, the mixing of baicalin equal quality ratio.
A kind of high-efficiency feed mold toxin sorbent preparation process, which is characterized in that the modified montmorillonite used mass fraction
30%, yeast cell wall mass fraction 55%, vitamin mass fraction account for 3%, probiotic powder mass fraction and account for 2%, natural plants and mention
It takes amount of substance score to account for 3%, minerals mass fraction and accounts for 7%, mix.
The probiotic powder is according to lactobacillus acidophilus: Lactobacillus amylophilus: saliva Lactobacillus lactis: streptococcus thermophilus: withered grass
Bacillus=4:4:3:3:3 ratio is mixed.
The lactobacillus acidophilus, Lactobacillus amylophilus, saliva Lactobacillus lactis, streptococcus thermophilus are separated pure by animal and bird intestines
Change amplification gained.
The natural plant extracts be by Honegsukle flower P.E, camellia oil extract, glycyrrhizic acid, Echinacea Purpurea Herb P.E,
Eugenol, cinnaldehydrum, the mixing of baicalin equal quality ratio.
A kind of high-efficiency feed mold toxin sorbent preparation process, which is characterized in that the modified montmorillonite used mass fraction
37%, yeast cell wall mass fraction 50%, vitamin mass fraction account for 1%, probiotic powder mass fraction and account for 2%, natural plants and mention
It takes amount of substance score to account for 4%, minerals mass fraction and accounts for 6%, mix.
The probiotic powder is according to lactobacillus acidophilus: Lactobacillus amylophilus: saliva Lactobacillus lactis streptococcus thermophilus: withered grass
Bacillus=4:4:3:3:3 ratio is mixed.
The lactobacillus acidophilus, Lactobacillus amylophilus, saliva Lactobacillus lactis, streptococcus thermophilus are separated pure by animal and bird intestines
Change amplification gained.
The natural plant extracts be by Honegsukle flower P.E, camellia oil extract, glycyrrhizic acid, Echinacea Purpurea Herb P.E,
Eugenol, cinnaldehydrum, the mixing of baicalin equal quality ratio.
A kind of high-efficiency feed mold toxin sorbent preparation process, which is characterized in that the modified montmorillonite used mass fraction
40%, yeast cell wall mass fraction 50%, vitamin mass fraction account for 1%, probiotic powder mass fraction and account for 1%, natural plants and mention
It takes amount of substance score to account for 3%, minerals mass fraction and accounts for 6%, mix.
The probiotic powder is according to lactobacillus acidophilus: Lactobacillus amylophilus: saliva Lactobacillus lactis: streptococcus thermophilus: withered grass
Bacillus=4:4:3:3:3 ratio is mixed.
The lactobacillus acidophilus, Lactobacillus amylophilus, saliva Lactobacillus lactis, streptococcus thermophilus are separated pure by animal and bird intestines
Change amplification gained.
The natural plant extracts be by Honegsukle flower P.E, camellia oil extract, glycyrrhizic acid, Echinacea Purpurea Herb P.E,
Eugenol, cinnaldehydrum, the mixing of baicalin equal quality ratio.
It is provided by the invention it is above-mentioned prepare mold toxin sorbent and in terms of preparation process can be applied to animal and fowl fodder,
It is applicable in various livestock and poultry cultivation feeds that above-mentioned mold toxin sorbent is added in livestock and poultry cultivation feed.
The mold toxin sorbent additive amount are as follows: 1Kg-5Kg is added in feed or raw material per ton.
The present invention provides a kind of high-efficiency feed mold toxin sorbent preparation process and has the advantage that
A kind of high-efficiency feed mold toxin sorbent preparation process, according to formula rate when production, required raw material is crushed,
It mixes, vacuum packaging.
The livestock and poultry mold toxin sorbent is raw materials used natural, pollution-free.
Livestock and poultry mold toxin sorbent provided by the present invention, first effectively can adsorb and degrade mycotoxin, subtract
Livestock and poultry can be enhanced to cultivation and the harm of growth of animals or poultry, the second adjustable animal and bird intestines environment, third in light mycotoxin
Abilities of digestive and absorption, the 4th can be enhanced the immunocompetence of livestock and poultry, the 5th the conversion ratio of feed can be improved, the 6th can inhibit
The growth of harmful bacteria.
A kind of high-efficiency feed mold toxin sorbent preparation process provided by the invention, the mold toxin sorbent be by
Modified montmorillonite used mass fraction accounts for 30%-40%, and yeast cell wall mass fraction accounts for 50%-55%, and vitamin mass fraction accounts for 1%-
3%, probiotic powder mass fraction accounts for 1%-2%, natural plant extracts mass fraction accounts for 3%-5%, minerals mass fraction accounts for 5%-
7% composition.The probiotic powder is by lactobacillus acidophilus, Lactobacillus amylophilus, saliva Lactobacillus lactis, streptococcus thermophilus, withered grass
Bacillus composition, the natural plant extracts are mentioned by Honegsukle flower P.E, camellia oil extract, glycyrrhizic acid, Echinacea
Object, eugenol, cinnaldehydrum, baicalin equal quality ratio is taken to mix.
The modified montmorillonite used mainly absorbing mycotoxin, reduces the absorption to nutriments such as amino acid, vitamins；
Yeast cell wall mainly utilizes its glucan and mannan structures efficient absorption mycotoxin, and in addition yeast cell wall can be with
Fowl poultry immune system is adjusted, the proliferation of beneficial bacteria of intestinal tract is promoted；The adjustable intestinal microenvironment of probiotic powder maintains livestock and poultry
Intestinal flora balance promotes the digestion and absorption of livestock and poultry；Vitamin is made of vitamin C and vitamin E, and vitamin C is a kind of
Important antioxidant, the lipid components that can be played on protection biomembrane are not oxidized, and alleviation stress be damaged caused by body
Wound, vitamin E can protect body tissue not by the infringement of free radical；The natural plant extracts, effect are mainly height
Effect releases and weakens the toxicity of mycotoxin, and certain protective effect is in addition also functioned to liver；Zinc methionine, on the one hand can be with
Essential amino acid methionine is provided, this makes great sense protection liver, and another aspect zinc has well to livestock and poultry power is improved
Booster action；Probiotic powder metabolism can produce some special enzyme systems, such as generates the enzyme system of organic acid, synthesizes the enzyme of polysaccharide
System, the enzyme system of decomposing lactic acid bacterium growth factor, the enzyme system of decomposing nitrosamine, the enzyme for reducing the enzyme system of cholesterol, task decomposition granularity
System, lipolytic enzyme system, the enzyme system of the various vitamins of synthesis and the enzyme system for decomposing cholic acid etc..These enzymes can not only accelerate lactic acid
The growth of bacillus maintains intestinal microecology balance, promotes body health, but also can improve the flavor of product, promotes product
Maturation with stablize.
Various raw materials used in the present invention can cooperate, and common efficient performance mycotoxin adsorption and degradation are made
With, mould contamination and mycotoxin contamination problem in feed and raw material can be effectively reduced, can effectively adsorb aspergillus flavus poison
A variety of toxin such as plain B1, zearalenone, Trichothecenes toxin, ochratoxin, fumarubicin, reduce because mould
Verticillium toxin pollutes the various cultivation problems of bring；Improve human body immune function；Intestinal flora balance is maintained, stomach, enteron aisle function are protected
It can be sound.
Livestock and poultry mold toxin sorbent usage amount provided by the invention is few, and palatability is good, can be uniform rapidly in feed
Mixing；Stability is good in processing and storing process, prevented from caking；Raw materials used is natural green material, for feed and is supported
Growing enterprise has good application value.
Specific embodiment
Above description content of the present invention is further detailed below by way of form of implementation, it is all above-mentioned based on the present invention
The application technology that content is realized is application range of the invention.
Zinc methionine, bacillus subtilis, yeast cell wall, vitamin C, vitamin E and experiment used in the present invention
Used reagent is market purchase in the process.
The present invention announced the specific process is as follows:
1, weighed respectively according to formula rate each in mold toxin sorbent, modified montmorillonite used, yeast cell wall, probiotic powder,
Natural plant extracts, vitamin, minerals, probiotic powder is according to lactobacillus acidophilus: Lactobacillus amylophilus: saliva yogurt bar
Bacterium, streptococcus thermophilus: bacillus subtilis=4:4:3:3:3 ratio is mixed, and natural plant extracts are mentioned by honeysuckle
Take object, camellia oil extract, glycyrrhizic acid, Echinacea Purpurea Herb P.E, eugenol, cinnaldehydrum, the mixing of baicalin equal quality ratio.
2, by yeast cells, probiotic powder, natural extract, minerals, be disposably added to according to per kilogram additive amount it is mixed
In clutch, mixed-powder No.1 is obtained.
3, Mixed Microbes powder No.1 is uniformly mixed on mixing machine with modified montmorillonite used, obtains the Mixed Microbes powder containing montmorillonite
No. two, 200 meshes were wanted before mixing wherein modified montmorillonite used, and environment temperature control is at 25 DEG C ± 2 DEG C when mixing, ambient humidity
Control is 55% ± 5%.
Wherein probiotic powder, lactobacillus acidophilus, Lactobacillus amylophilus, saliva Lactobacillus lactis, streptococcus thermophilus are from livestock and poultry
Isolated strain, process are as follows in intestinal contents:
1, in each section of content of healthy aquaculture animal gastrointestinal tract bacterial strain separation and screening: by it is sterile take healthy growth pig and
Fowl jejunum and caecum sample, are then cultivated by selective medium separation, expansion, inoculation, expand the behaviour substantially such as culture again
Make, extraction purification bacterial strain DNA simultaneously carries out PCR amplification to 16SrDNA, and sequencing identifies aimed strain, then carries out repeatedly expanding training
It supports.It filters out and plays the object bacteria of prebiotic effect in each site of enteron aisle.
2, the fermentation liquid for separating each bacterial strain one time fermentation is inoculated into the secondary culture that spreads cultivation by the fermented and cultured of object bacteria respectively
In base, 40 DEG C, constant temperature stationary culture for 24 hours.
3, it is spray-dried condensation method, micro-capsule coating is carried out to each bacterial strain, (1) prepares core material mixture and (is with alginate
Example)；(2) mixture enters atomizer and is atomized under the conditions of certain pressure；(3) liquid is received with calcium chloride (0.5mol/L) after being atomized
Collect slot hardening；(4) it washs, be transferred to packaging material (by taking chitosan as an example) lactic acid solution and be uniformly coated with；(5) by microcapsules after (4) processing
Filtration washing, freeze-drying.It repeats (1)-(5), carries out the screening of more than 30 kinds of core materials and packaging material combination, determine most preferably to be atomized
Pressure, the parameters such as packaging material.
Wherein, natural plant extracts cinnaldehydrum " surpasses referring to what Hu Shuchen et al. was delivered in optimization of orthogonal test FRUCTUS CINNAMOMI CASSIAE LMMA-TURI
The technique of sound extraction cinnaldehydrum " (" Xinjiang Medicine University's journal " 2013,36 (9): 1278-1281).
Wherein, natural plant extracts Echinacea Purpurea Herb P.E, referring to once found the state chief editor " plant extracts standardization grind
Study carefully --- method and demonstration " (Beijing: Chemical Industry Press, 2011.10:305-308).
Wherein, natural plant extracts baicalin referring to Yuan Mengzhe et al. deliver " supercritical ultrasonics technology extracts Huang from radix scutellariae
The Orthogonal Experiment and Design of Siberian cocklebur glycosides " (" mathematics medicine and pharmacology magazine ", 2007,20 (1): 78-79).
Wherein, natural plant extracts glycyrrhizic acid " extracts the new work of glycyrrhizic acid referring to what Li Shanjia et al. was delivered in Radix Glycyrrhizae
Skill research " (" Gansu science journal ", 2010,22 (2): 53-57).
Wherein, " the preferred gold and silver of orthogonal experiment that natural plant extracts Honegsukle flower P.E is delivered referring to Liu Gang et al.
Colored Study on extraction " (" Strait Pharmaceutical Journal ", 2012,24 (8): 17-19).
Wherein, " the optimal extracting method and pharmacology of eugenol that natural plant extracts eugenol is delivered referring to An Tongwei
Effect " (" Shandong animal and veterinary ", 2013,34 (2): 5-7).
A kind of livestock and poultry mold toxin sorbent that the present invention is prepared by the above method, there is mycotoxin and growth of animals or poultry
Good effect.
Case study on implementation 1
The formula that the implementation case prepares mold toxin sorbent is as follows:
It is respectively as follows: according to quality specific gravity and weighs modified montmorillonite used 3Kg, 5.5 Kg of yeast cell wall, vitamin 0.3Kg, probiotics
0.2 Kg of powder, 0.3 Kg of natural plant extracts, 0.7 Kg of minerals.
Production method: at 25 DEG C ± 2 DEG C of environment temperature, under the conditions of ambient humidity 55% ± 5%, according to formula rate, mix,
Each raw material is mixed, is finally vacuum-packed.
Application method: by mold toxin sorbent prepared by the present invention according to 0.1% ratio (feed addition 1Kg i.e. per ton
Mold toxin sorbent) be added in complete feed, while using be not added with mold toxin sorbent group as control.
Case study on implementation 2
The formula that the implementation case prepares mold toxin sorbent is as follows:
It is respectively as follows: according to quality specific gravity and weighs modified montmorillonite used 3.7Kg, 5 Kg of yeast cell wall, vitamin 0.1Kg, probiotics
0.2 Kg of powder, 0.4 Kg of natural plant extracts, minerals 0.6Kg.
Preparation method and application method are the same as case study on implementation 1.
Case study on implementation 3
It is respectively as follows: according to quality specific gravity and weighs modified montmorillonite used 4Kg, 5 Kg of yeast cell wall, vitamin 0.1Kg, probiotic powder
0.1 Kg, 0.3 Kg of natural plant extracts, minerals 0.6Kg.
Preparation method and application method are the same as case study on implementation 1.
Applicant is by a large amount of experiment and confirmatory experiment, according to the livestock and poultry mycotoxin of formula preparation provided by the invention
Adsorbent can be applied in feed and add.The experimental results showed that the mold toxin sorbent that the present invention is announced is to common
Mycotoxin has good adsorption effect, tests to piglet feed, has good production capacity effect, reduces disease incidence, improves
Daily gain reduces material ratio, and following data can be used as the data supporting of effect description.
Test result explanation:
Table 1 is using addition mold toxin sorbent in complete diet pellet to aflatoxin, vomitoxin, T-2 toxin, Aspergillus ochraceus poison
The absorption situation of element, zearalenone utilizes calibration curve method mark using thunder Du's R6000 microplate reader light absorption value at 450nm
Determine the pollution condition of mycotoxin.
Table 1 is changed using the Mycotoxins in Feed pollution condition of mold toxin sorbent
| Project | Aflatoxin | Vomitoxin | T-2 toxin | Zearalenone | Ochratoxin |
| Case study on implementation 1 | 4.5495ppb | 0.5236ppm | 0.781ppm | 478.374ppb | 48.6638ppb |
| Case study on implementation 2 | 6.7893ppb | 0.6893ppm | 0.6538ppm | 395.4665ppb | 43.5509ppb |
| Case study on implementation 3 | 5.7658ppb | 0.5574ppm | 0.5953ppm | 450.78ppb | 50,7859ppb |
| Control | 18.6523ppb | 1.7901ppm | 1.2703ppm | 1297.1034ppb | 150.6433ppb |
As it can be seen from table 11,2,3 pair of 5 kinds of common mycotoxin of case study on implementation have an absorption well, it can be significant
Reduction Mycotoxins in Feed contents level.
In order to verify the effect for the mold toxin sorbent that the present invention announces, applicant is in July, 2018 on pig farm
The nursing carried out 2 weeks by a definite date is tested, and 30 age in days piglets is randomly divided into 3 experimental groups and 1 control group in test, every group includes
Piglet 30.
Feeding method: control group feeds basal diet；Case study on implementation 1,2,3 is added in the basal diet of experimental group feeding
The mold toxin sorbent of preparation.
Statistical method: piglet daily gain, feedstuff-meat ratio, disease incidence, the death rate are counted by 30 ages in days until 60 ages in days, are adopted
Experimental result is compared with statistical method.
Influence of the table 2 using the agent of mycotoxin De contamination to weaned piglets
| Test group | Disease incidence | The death rate | Daily gain (g/d) | Feedstuff-meat ratio |
| Case study on implementation 1 | 3.33% | 0 | 383.74±10 | 1.57 |
| Case study on implementation 2 | 0 | 0 | 425.84±10 | 1.45 |
| Case study on implementation 3 | 0 | 0 | 415.68±10 | 1.50 |
| Control | 58.33% | 5.8% | 280.66±10 | 2.10 |
By table 2, by comparing experimental group and control group, it can significantly find out that the mold toxin sorbent that the present invention is announced is made
It is obvious with effect, the feed of mold toxin sorbent is added, weaned piglets function and effect are good, and disease incidence is low, daily gain
Increase obviously compared with control group, can significantly reduce material ratio, improve the conversion ratio of feed.
In case study on implementation, 2,3 pairs of weaned piglets function and effect of case study on implementation are better than implementation as can be seen from Table 2
Case 1.
Claims (3)
1. a kind of high-efficiency feed mold toxin sorbent preparation process of claim, it is characterised in that: modified montmorillonite used quality point
Number accounts for 30%-40%, and yeast cell wall mass fraction accounts for 50%-55%, and vitamin mass fraction accounts for 1%-3%, probiotic powder quality point
Number accounts for 1%-2%, natural plant extracts mass fraction accounts for 3%-5%, minerals mass fraction accounts for 5%-7%, and the natural plants mention
Taking object is by Honegsukle flower P.E, camellia oil extract, glycyrrhizic acid, Echinacea Purpurea Herb P.E, eugenol, cinnaldehydrum, baicalin etc.
Mass ratio mixing.
2. a kind of high-efficiency feed mold toxin sorbent preparation process according to claim 1, which is characterized in that the benefit
Raw bacterium powder isolates and purifies expansion by animal and bird intestines by lactobacillus acidophilus, Lactobacillus amylophilus, saliva Lactobacillus lactis, streptococcus thermophilus
Increase gained.
3. a kind of high-efficiency feed mold toxin sorbent preparation process according to claim 1 to 2, which is characterized in that
The probiotic powder is according to lactobacillus acidophilus: Lactobacillus amylophilus: saliva Lactobacillus lactis: streptococcus thermophilus: bacillus subtilis
The ratio of=4:4:3:3:3 is mixed.
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| CN115067458A (en) * | 2022-06-22 | 2022-09-20 | 浙江启润生物科技有限公司 | Biodegradation method of mycotoxin in feed raw materials |
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