CN115428862A - Antibacterial product produced by bacteriophage and lyase and preparation method thereof - Google Patents

Antibacterial product produced by bacteriophage and lyase and preparation method thereof Download PDF

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Publication number
CN115428862A
CN115428862A CN202110614799.3A CN202110614799A CN115428862A CN 115428862 A CN115428862 A CN 115428862A CN 202110614799 A CN202110614799 A CN 202110614799A CN 115428862 A CN115428862 A CN 115428862A
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China
Prior art keywords
parts
lyase
powder
phage
bacteriophage
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Inventor
何四龙
丛郁
徐旭凌
乔欢
肖逍
许文建
丁良
于浩
樊小九
徐天舜
许小康
李涛
华雨田
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Phagelux Nanjing Biotechnology Co ltd
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Phagelux Nanjing Biotechnology Co ltd
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Priority to CN202110614799.3A priority Critical patent/CN115428862A/en
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
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    • A23K20/10—Organic substances
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    • A—HUMAN NECESSITIES
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    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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Abstract

The invention discloses an antibacterial product produced by phage and lyase and a preparation method thereof, wherein the antibacterial product comprises the following raw material components: 200-400 parts of illite powder, 150-250 parts of mica powder, 150-250 parts of montmorillonite powder, 40-70 parts of phage lyase, 40-60 parts of a body-strengthening agent, 10-30 parts of a vitamin supplement, 10-30 parts of a Chinese herbal medicine additive, 6-12 parts of a reinforcing absorbent and 300-450 parts of water. Has the beneficial effects that: the invention can adsorb mycotoxin, endotoxin and enterotoxin, can avoid feed mildew in the breeding industry, can eliminate staphylococcus and escherichia coli in animal bodies, reduces the probability of diseases and inflammations, and does not generate drug resistance of bacteria.

Description

Antibacterial product produced by bacteriophage and lyase and preparation method thereof
Technical Field
The invention relates to the field of bacteriophage antibacterial products, and particularly relates to an antibacterial product produced by using bacteriophage and lyase and a preparation method thereof.
Background
The phage is a virus specially used for cracking bacteria, and the main chemical components of the phage consist of protein and nucleic acid and are harmless to human bodies. The antibacterial products produced by the bacteriophage and the lyase can be applied to the fields of human health, animal culture, crop health, food safety and the like.
At present, in the published patent of the antibacterial products and the existing antibacterial products in the market, for example, chinese patent No. CN103989728B, a compound medicine containing zhu cao and beta-lactam antibacterial drugs for livestock and poultry is disclosed. The raw materials comprise beta-lactam antibacterial drugs and phyllanthus urinaria, and after the phyllanthus urinaria is added, the drug resistance of bacteria to the antibacterial drugs can be reversed, so that the treatment effect of the antibacterial drugs is improved. It does not completely address bacterial resistance per se. After long-term use of the antibacterial product, the effect is poor, and the yield and the quality of animals are affected. And the ability to adsorb mycotoxins, endotoxins and enterotoxins is lacking, affecting the survival rate of animals.
An effective solution to the problems in the related art has not been proposed yet.
Disclosure of Invention
Aiming at the problems in the related art, the invention provides an antibacterial product produced by bacteriophage and lyase and a preparation method thereof, so as to overcome the technical problems in the prior related art.
Therefore, the invention adopts the following specific technical scheme:
according to one aspect of the invention, there is provided an antimicrobial product produced using bacteriophage and a lytic enzyme.
The antibacterial product produced by using the bacteriophage and the lyase comprises the following raw material components: 200-400 parts of illite powder, 150-250 parts of mica powder, 150-250 parts of montmorillonite powder, 40-70 parts of phage lyase, 40-60 parts of a body-strengthening agent, 10-30 parts of a vitamin supplement, 10-30 parts of a Chinese herbal medicine additive, 6-12 parts of an enhanced absorbent and 300-450 parts of water.
Further, the phage lyase comprises the following raw material components: 20-35 parts of staphylococcus lyase and 20-35 parts of escherichia coli lyase.
Further, the body strengthening agent comprises the following raw material components: 20-30 parts of soybean protein isolate, 10-15 parts of bagasse, 5-7.5 parts of spleen aminopeptide freeze-dried powder and 5-7.5 parts of ginseng extract powder.
Further, the vitamin supplement comprises at least one of sophora flower, lemongrass, raspberry, medlar and mulberry leaves.
Further, the Chinese herbal medicine additive comprises at least one of dandelion, pig sprout, honeysuckle, sweet wormwood, forsythia and plantain herb.
Further, the reinforced absorbent comprises the following raw material components: 2-4 parts of lactobacillus plantarum, 2-4 parts of streptococcus thermophilus and 2-4 parts of lactobacillus bulgaricus.
According to another aspect of the present invention, there is provided a method for producing an antibacterial product using bacteriophage and a lytic enzyme.
The preparation method for producing the antibacterial product by using the bacteriophage and the lyase comprises the following steps of:
freeze-drying the staphylococcus lyase and the escherichia coli lyase into protein freeze-dried powder according to the parts, and storing for later use;
weighing other raw materials according to the parts, naturally drying the vitamin supplement and the Chinese herbal medicine additive, and then putting into a grinder for grinding to obtain a Chinese herbal medicine mixture;
adding phage lyase, a Chinese herbal medicine mixture, illite powder, mica powder, montmorillonite powder, a body strengthening agent, an enhanced absorbent and water into a stirrer and stirring to obtain an antibacterial product;
the antimicrobial product is added to the feed of the feeding animal.
Further, when the staphylococcus lyase and the escherichia coli lyase are freeze-dried into the protein freeze-dried powder, calcium ions are added into the protein freeze-dried powder, and the concentration of the calcium ions is ensured to be 50-800 mu M when the lyase works.
Furthermore, after the vitamin supplement and the Chinese herbal medicine additive are naturally dried, the vitamin supplement and the Chinese herbal medicine additive are placed into a grinder to be ground, and the mesh number of a screen plate of the grinder is 40 meshes.
Further, when the phage lyase, the Chinese herbal medicine mixture, the illite powder, the mica powder, the montmorillonite powder, the body strengthening agent, the reinforcing absorbent and the water are added into a stirrer to be stirred for 0.5-1h
The beneficial effects of the invention are as follows: according to the invention, the illite powder, the mica powder and the montmorillonite powder are added, so that mycotoxin, endotoxin and enterotoxin can be adsorbed, the feed in the breeding industry can be prevented from mildewing, and further, the toxin can be prevented from entering animal viscera, so that the yield and quality of the breeding industry are reduced, and the health of human beings is harmed; by adding the staphylococcus lyase and the escherichia coli lyase, the staphylococcus and the escherichia coli in an animal body can be eliminated, the probability of diseases and inflammation is reduced, and the drug resistance of bacteria cannot be generated; by adding the vitamin supplement and the Chinese herbal medicine additive, vitamins can be provided for the cultured animals, and the antibacterial ability is improved. By adding the body strengthening agent and the absorption enhancing agent, protein can be provided for the cultured animals, the absorption capacity of intestines and stomach of the animals is improved, and the yield of the cultured animals is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a method for producing an antibacterial product using bacteriophage and a lytic enzyme according to an embodiment of the present invention.
Detailed Description
For further explanation of the various embodiments, the drawings which form a part of the disclosure and which are incorporated in and constitute a part of this specification, illustrate embodiments and, together with the description, serve to explain the principles of operation of the embodiments, and to enable others of ordinary skill in the art to understand the various embodiments and advantages of the invention, and, by reference to these figures, reference is made to the accompanying drawings, which are not to scale and wherein like reference numerals generally refer to like elements.
According to an embodiment of the present invention, there is provided a method for producing an antibacterial product using bacteriophage and a lytic enzyme.
The antibacterial product produced by using the phage and the lyase comprises the following raw material components: 200-400 parts of illite powder, 150-250 parts of mica powder, 150-250 parts of montmorillonite powder, 40-70 parts of phage lyase, 40-60 parts of a body-strengthening agent, 10-30 parts of a vitamin supplement, 10-30 parts of a Chinese herbal medicine additive, 6-12 parts of a reinforcing absorbent and 300-450 parts of water.
Wherein the phage lyase comprises the following raw material components: 20-35 parts of staphylococcus lyase and 20-35 parts of escherichia coli lyase.
The body strengthening agent comprises the following raw material components: 20-30 parts of soybean protein isolate, 10-15 parts of bagasse, 5-7.5 parts of spleen aminopeptide freeze-dried powder and 5-7.5 parts of ginseng extract powder.
Wherein the vitamin supplement comprises at least one of flos Sophorae Immaturus, herba Cymbopogonis Citrari, rubi fructus, fructus Lycii and folium Mori.
Wherein the Chinese herbal medicine additive comprises at least one of herba Taraxaci, fructus Gei Piloselloidis, flos Lonicerae, herba Artemisiae Annuae, fructus forsythiae and herba plantaginis.
Wherein, the reinforced absorbent comprises the following raw material components: 2-4 parts of lactobacillus plantarum, 2-4 parts of streptococcus thermophilus and 2-4 parts of lactobacillus bulgaricus.
In order to clearly understand the technical scheme of the invention, the technical scheme of the invention is described in detail by specific examples.
Example 1
An antibacterial product produced by using phage and lyase is prepared from the following raw materials in parts by weight:
200g of illite powder, 150g of mica powder, 150g of montmorillonite powder, 40g of phage lyase, 40g of a body-strengthening agent, 10g of a vitamin supplement, 10g of a Chinese herbal medicine additive, 6g of a reinforcing absorbent and 300g of water.
The phage lyase comprises the following raw material components: 20g of staphylococcus lyase and 20g of escherichia coli lyase.
The body strengthening agent comprises the following raw material components: 20g of soybean protein isolate, 10g of bagasse, 5g of spleen aminopeptide freeze-dried powder and 5g of ginseng extract powder.
The vitamin supplement comprises at least one of flos Sophorae Immaturus, herba Cymbopogonis Citrari, rubi fructus, fructus Lycii and folium Mori.
The Chinese herbal medicine additive comprises at least one of herba Taraxaci, pig sprout, flos Lonicerae, herba Artemisiae Annuae, fructus forsythiae and herba plantaginis.
The reinforced absorbent comprises the following raw material components: 2g of lactobacillus plantarum, 2g of streptococcus thermophilus and 2g of lactobacillus bulgaricus.
The preparation method for producing the antibacterial product by using the phage and the lyase comprises the following steps:
freeze-drying 20g of staphylococcus lyase and 20g of escherichia coli lyase into protein freeze-dried powder according to the parts, and storing for later use;
weighing other raw materials according to the parts, naturally drying 10g of vitamin supplement and 10g of Chinese herbal medicine additive, and then putting into a grinder for grinding to obtain a Chinese herbal medicine mixture;
adding 40g of phage lyase, 20g of Chinese herbal medicine mixture, 200g of illite powder, 150g of mica powder, 150g of montmorillonite powder, 40g of body-strengthening agent, 6g of reinforcing absorbent and 300g of water into a stirrer and stirring to obtain an antibacterial product;
the antimicrobial product is added to the feed of the feeding animal.
Example 2
An antibacterial product produced by using phage and lyase is prepared from the following raw materials in parts by weight:
300g of illite powder, 200g of mica powder, 200g of montmorillonite powder, 55g of phage lyase, 50g of a body-strengthening agent, 20g of a vitamin supplement, 20g of a Chinese herbal medicine additive, 9g of a reinforcing absorbent and 375g of water.
The phage lyase comprises the following raw material components: 27.5g of staphylococcal lyase and 27.5g of Escherichia coli lyase.
The body strengthening agent comprises the following raw material components: 25g of soybean protein isolate, 12.5g of bagasse, 6.25g of spleen aminopeptide freeze-dried powder and 6.25g of ginseng extract powder.
The vitamin supplement comprises at least one of flos Sophorae Immaturus, herba Cymbopogonis Citrari, rubi fructus, fructus Lycii and folium Mori.
The Chinese herbal medicine additive comprises at least one of herba Taraxaci, fructus Hordei Germinatus, flos Lonicerae, herba Artemisiae Annuae, fructus forsythiae and herba plantaginis.
The reinforced absorbent comprises the following raw material components: 3g of lactobacillus plantarum, 3g of streptococcus thermophilus and 3g of lactobacillus bulgaricus.
The preparation method for producing the antibacterial product by using the phage and the lyase comprises the following steps:
freeze-drying 27.5g of staphylococcus lyase and 27.5g of escherichia coli lyase into protein freeze-dried powder according to the parts, and storing for later use;
weighing other raw materials according to the parts by weight, naturally drying 20g of vitamin supplement and 20g of Chinese herbal medicine additive, and then putting into a grinder for grinding to obtain a Chinese herbal medicine mixture;
adding 55g of phage lyase, 40g of Chinese herbal medicine mixture, 300g of illite powder, 200g of mica powder, 200g of montmorillonite powder, 50g of body-strengthening agent, 9g of reinforcing absorbent and 375g of water into a stirrer and stirring to obtain an antibacterial product;
the antimicrobial product is added to the feed of the feeding animal.
Example 3
An antibacterial product produced by bacteriophage and lyase is prepared from the following raw materials in parts by weight:
400g of illite powder, 250g of mica powder, 250g of montmorillonite powder, 70g of phage lyase, 60g of a body-strengthening agent, 30g of a vitamin supplement, 30g of a Chinese herbal medicine additive, 12g of a reinforcing absorbent and 450g of water.
The phage lyase comprises the following raw material components: 35g of staphylococcus lyase and 35g of escherichia coli lyase.
The body strengthening agent comprises the following raw material components: 30g of soybean protein isolate, 15g of bagasse, 7.5g of spleen aminopeptide freeze-dried powder and 7.5g of ginseng extract powder.
The vitamin supplement comprises at least one of flos Sophorae Immaturus, herba Cymbopogonis Citrari, rubi fructus, fructus Lycii and folium Mori.
The Chinese herbal medicine additive comprises at least one of herba Taraxaci, pig sprout, flos Lonicerae, herba Artemisiae Annuae, fructus forsythiae and herba plantaginis.
The reinforced absorbent comprises the following raw material components: 4g of lactobacillus plantarum, 4g of streptococcus thermophilus and 4g of lactobacillus bulgaricus.
The preparation method for producing the antibacterial product by using the bacteriophage and the lyase comprises the following steps:
freeze-drying 35g of staphylococcus lyase and 35g of escherichia coli lyase into protein freeze-dried powder according to the parts, and storing for later use;
weighing other raw materials according to the parts, naturally drying 30g of vitamin supplement and 30g of Chinese herbal medicine additive, and then putting into a grinder for grinding to obtain a Chinese herbal medicine mixture;
adding 70g of phage lyase, 60g of Chinese herbal medicine mixture, 400g of illite powder, 250g of mica powder, 250g of montmorillonite powder, 60g of body-strengthening agent, 12g of reinforcing absorbent and 450g of water into a stirrer and stirring to obtain an antibacterial product;
the antimicrobial product is added to the feed of the feeding animal.
Comparative example
In comparison with example 1, the antibacterial product used in the comparative example was prepared using a formulation that is common in the existing market.
The antibacterial products prepared in examples 1 to 3 and the comparative example were added to the same specification of aflatoxin B1, fumonisin and T2 toxin petri dishes, respectively, and the obtained performance data are shown in table 1 below.
TABLE 1
Figure BDA0003097628980000071
From the above table, the antibacterial product produced by the phage and the lyase is obviously better than the comparative product in the adsorption rate of aflatoxin B1, fumonisin and T2 toxin.
For convenience of understanding the above technical solution of the present invention, the following detailed description is made on the flow of the above technical solution of the present invention with reference to the accompanying drawings, and specifically is as follows:
according to an embodiment of the present invention, there is also provided a method for producing an antibacterial product using a bacteriophage and a lytic enzyme.
As shown in FIG. 1, the preparation of the antibacterial product using bacteriophage and lyase in the actual production process includes the following steps:
s101, freeze-drying staphylococcus lyase and escherichia coli lyase into protein freeze-dried powder according to the parts, and storing for later use;
s102, weighing other raw materials according to the parts, naturally drying the vitamin supplement and the Chinese herbal medicine additive in the air, and then putting the mixture into a grinder to be ground to obtain a Chinese herbal medicine mixture;
s103, adding phage lyase, a Chinese herbal medicine mixture, illite powder, mica powder, montmorillonite powder, a body strengthening agent, a reinforcing absorbent and water into a stirrer and stirring to obtain an antibacterial product;
s104, adding the antibacterial product into the feed for feeding animals.
In one embodiment, when the staphylococcus lyase and the escherichia coli lyase are lyophilized into the protein lyophilized powder, calcium ions are added into the protein lyophilized powder, and the calcium ions are added in an amount which ensures that the concentration of the calcium ions is 50-800 μ M when the lyase works.
In one embodiment, the vitamin supplement and the herbal additive are naturally air-dried and then placed into a pulverizer, and the mesh size of the pulverizer is 40 meshes.
In one embodiment, the phage lyase, the Chinese herbal medicine mixture, the illite powder, the mica powder, the montmorillonite powder, the body strengthening agent, the absorption enhancing agent and the water are added into a stirrer and stirred for 0.5-1h.
In conclusion, by means of the technical scheme, the illite powder, the mica powder and the montmorillonite powder are added, so that mycotoxin, endotoxin and enterotoxin can be adsorbed, feed mildew in the breeding industry can be avoided, and further, toxin can be prevented from entering animal internal organs to cause the reduction of the yield and quality of the breeding industry and harm to human health; by adding the staphylococcus lyase and the escherichia coli lyase, the staphylococcus and the escherichia coli in an animal body can be eliminated, the probability of diseases and inflammation is reduced, and the drug resistance of bacteria cannot be generated; by adding the vitamin supplement and the Chinese herbal medicine additive, vitamins can be provided for the cultured animals, and the antibacterial ability is improved. By adding the body strengthening agent and the absorption enhancing agent, protein can be provided for the cultured animals, the absorption capacity of intestines and stomach of the animals is improved, and the yield of the cultured animals is improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. An antibacterial product produced by phage and lyase is characterized by comprising the following raw materials: 200-400 parts of illite powder, 150-250 parts of mica powder, 150-250 parts of montmorillonite powder, 40-70 parts of phage lyase, 40-60 parts of a body-strengthening agent, 10-30 parts of a vitamin supplement, 10-30 parts of a Chinese herbal medicine additive, 6-12 parts of a reinforcing absorbent and 300-450 parts of water.
2. The method of claim 1, wherein the phage lytic enzyme comprises the following components: 20-35 parts of staphylococcus lyase and 20-35 parts of escherichia coli lyase.
3. The method for producing an antibacterial product by using bacteriophage and lyase according to claim 2, wherein the body-strengthening agent comprises the following raw material components: 20-30 parts of soybean protein isolate, 10-15 parts of bagasse, 5-7.5 parts of spleen aminopeptide freeze-dried powder and 5-7.5 parts of ginseng extract powder.
4. The method of claim 3, wherein the vitamin supplement comprises at least one of Sophora japonica, ruta graveolens, rubi fructus, lycium Chinense, and Morus alba L.
5. The method for producing an antibacterial product using bacteriophage and lyase as claimed in claim 4, wherein the herbal supplement comprises at least one of dandelion, pig sprout, honeysuckle, sweet wormwood, forsythia and plantain herb.
6. The method for producing antibacterial products using bacteriophage and lytic enzyme according to claim 5, wherein the absorbent-enhancing agent comprises the following raw material components: 2-4 parts of lactobacillus plantarum, 2-4 parts of streptococcus thermophilus and 2-4 parts of lactobacillus bulgaricus.
7. A method for producing an antibacterial product using a bacteriophage and a lytic enzyme, which is used for the production of the antibacterial product using a bacteriophage and a lytic enzyme according to claim 6, comprising the steps of:
freeze-drying the staphylococcus lyase and the escherichia coli lyase into protein freeze-dried powder according to the parts, and storing and reserving the protein freeze-dried powder for later use;
weighing other raw materials according to the parts, naturally drying the vitamin supplement and the Chinese herbal medicine additive, and then putting into a grinder for grinding to obtain a Chinese herbal medicine mixture;
adding phage lyase, a Chinese herbal medicine mixture, illite powder, mica powder, montmorillonite powder, a body strengthening agent, a reinforcing absorbent and water into a stirrer and stirring to obtain an antibacterial product;
the antimicrobial product is added to the feed of the feeding animal.
8. The method of claim 7, wherein the step of lyophilizing the staphylococcal lyase and the Escherichia coli lyase to obtain a lyophilized protein powder further comprises adding calcium ions to the lyophilized protein powder, wherein the calcium ions are added to ensure that the concentration of the calcium ions is 50-800 μ M when the lyase is operated.
9. The method of claim 7, wherein the vitamin supplement and the herbal supplement are naturally dried and crushed in a crusher, the mesh size of the crusher is 40.
10. The method of claim 7, wherein the phage lytic enzyme, the herbal mixture, the illite powder, the mica powder, the montmorillonite powder, the body-strengthening agent, the absorbent and the water are added into a mixer and mixed for 0.5-1h.
CN202110614799.3A 2021-06-02 2021-06-02 Antibacterial product produced by bacteriophage and lyase and preparation method thereof Pending CN115428862A (en)

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