CN113057253B - Composite probiotic pet food for coordinating intestines and stomach and preparation method thereof - Google Patents

Abstract

The invention relates to a composite probiotic pet food for coordinating intestines and stomach and a preparation method thereof, belonging to the technical field of pet foods. According to the invention, the probiotic additive is prepared by self, and the specific traditional Chinese medicine extract and catechin are matched to perform a synergistic effect with each other, so that beneficial microorganisms in the pet intestinal tract can be proliferated, a harmful bacterium in the intestinal tract can be inhibited, the microbial population structure and the number in the pet intestinal tract are finally improved, the pet intestine and stomach are effectively regulated, and the application prospect is wide.

Description

Composite probiotic pet food for coordinating intestines and stomach and preparation method thereof

Technical Field

The invention relates to a composite probiotic pet food for coordinating intestines and stomach and a preparation method thereof, belonging to the technical field of pet foods.

Background

At present, with the increasing volume of pets, dog food, cat food and the like which are eaten by domestic pets for a long time are all processed foods, and the processed foods contain food additives, so that the pets take various food additives for a long time, and gastrointestinal diseases of the pets are caused; meanwhile, due to the wide use of antibiotics, the normal flora of the intestinal tract of the pet is disordered by the drug resistance and the drug residue of the organism, so that the pet is easy to suffer from intestinal diseases such as constipation, diarrhea, dyspepsia, gastroenteritis and the like, and the health of the pet is harmed.

More than 85% of diarrhea of pets is caused by intestinal microecological imbalance, and can be recovered only by correcting the microecological balance without antibiotic treatment, and how to correct the microecological balance is one of the problems to be solved urgently. Serious intestinal diseases, when treated by antibiotics, kill or inhibit harmful flora and most beneficial flora in the intestinal tract. After the antibiotics are stopped, when the intestinal microecological balance is reestablished in the intestinal tract, the harmful flora is possibly dominant, and the intestinal infection is caused again, so that the serious intestinal diseases are treated by the antibiotics in the recovery stage, how to take the intestinal care, promote the proliferation of the intestinal beneficial flora and restore the intestinal microecological balance is one of the problems to be solved urgently.

The probiotic preparation is a natural active microbial preparation, and has the characteristics of no toxicity, no drug resistance, no residue and no side effect. The probiotics can competitively inhibit pathogenic bacteria in the animal digestive tract in a live bacterial form, thereby enhancing the immunity of the animal body, and directly participate in the balance of the intestinal microecology of the animal, thereby maintaining the normal function of the intestinal tract.

In view of the above drawbacks, the designer actively makes research and innovation to create a composite probiotic pet food for regulating intestines and stomach and a preparation method thereof, so that the pet food has industrial utilization value.

Disclosure of Invention

In order to solve the technical problems, the invention aims to provide a composite probiotic pet food for coordinating intestines and stomach and a preparation method thereof.

The invention relates to a composite probiotic pet food for coordinating intestines and stomach, which comprises the following raw materials in parts by weight:

10-14 parts of taurine;

28-32 parts of a self-made probiotic additive;

1-2 parts of vitamin C;

3-7 parts of chelated calcium powder;

the self-made probiotic additive is prepared by mixing a self-made probiotic carrier and composite probiotic powder;

the self-made probiotic carrier is prepared by the reaction of corncobs, a sodium hydroxide solution and an ethanol solution; the xylo-oligosaccharide in the corn cob is extracted by alkali, the low xylan component in the reactant is precipitated by alcohol precipitation, and then the mixed powder of the corn cob fiber and the corn cob low xylan is obtained by filtration and interception, the mixed powder is rich in the fiber and the low xylan component, has large specific surface area, can contain a large amount of probiotics and becomes a load carrier of the probiotics, the survival rate of the probiotics is ensured, in addition, the fiber is of a polyhydroxy structure and has strong water absorption, and the fiber can absorb the water in the intestinal tract of a diarrhea pet, so that the excrement volume is increased and is easier to discharge, a primary effect is generated for conditioning the intestinal tract of the pet, more importantly, the low xylan component can effectively inhibit the survival of harmful bacteria escherichia coli in the intestines and stomach, the harmful bacteria escherichia coli can generate stress metabolism, a large amount of glucuronidase is further generated, preparation is made for later-stage plant saponin enzymolysis, and the normal metabolic propagation of harmful microorganisms in the intestines and stomach can be inhibited by the low xylan, can be used as a binding receptor of pathogenic bacteria to competitively bind with phytohemagglutinin (or carbohydrate binding protein) on the cell surface of the pathogenic bacteria, reduces the chance of binding the pathogenic bacteria with intestinal mucosa epithelial cells, greatly reduces the pathogenic capability, plays a role in regulating the intestinal tract, simultaneously can also reduce the pH of the intestinal tract, promotes the mineral solubility to be increased, further increases the absorption and utilization of mineral electrolyte components of diarrhea animals, and further regulates the gastrointestinal function of pets, but xylooligosaccharide has selective proliferation effect on the beneficial bacteria screened by the invention, because the xylooligosaccharide has special beta-1, 4-glycosidic bond, can not be decomposed by animal endogenous enzyme, can not be utilized by harmful microorganisms, can directly reach the tail end of the small intestine and the large intestine, can be absorbed and utilized by the probiotic bacteria screened by the invention, promotes the large proliferation of the beneficial bacteria, and increases short chain fatty acid, and the like, The secretion of acetic acid and lactic acid stimulates the peristalsis of intestinal tracts, changes osmotic pressure, increases the water content of excrement and prevents gastrointestinal diseases such as constipation, diarrhea and the like;

the composite probiotic powder comprises the following raw materials in parts by weight:

5-9 parts of Bacillus belgii powder;

16-20 parts of lactobacillus reuteri powder;

7-11 parts of enterococcus hirae bacterial powder;

10-14 parts of bifidobacterium powder;

9-13 parts of rhamnose bacillus powder.

Further, the composite probiotic powder comprises the following raw materials in parts by weight:

7 parts of Bacillus belgii powder;

18 parts of lactobacillus reuteri powder;

9 parts of enterococcus hirae powder;

12 parts of bifidobacterium powder;

and mixing 11 parts of rhamnose bacillus powder to obtain the composite probiotic powder.

The self-made probiotic additive has the advantages that the weight ratio of various probiotics is optimal, and only under the optimal ratio, the interspecies competition among the probiotics and the decomposition effect of subsequent glucoside can reach the optimal, so that the optimal conditioning effect of the probiotics is realized;

further, the coating also comprises the following components in parts by weight:

13-17 parts of traditional Chinese medicine extraction powder;

the Chinese medicinal extract powder is prepared from radix Platycodi, cortex et radix Polygalae, rhizoma anemarrhenae, deionized water, diethyl ether and n-butanol by reacting. The invention removes fat-soluble impurities in the traditional Chinese medicine components by ethyl ether, then uses n-butanol to leach saponin active ingredients in the traditional Chinese medicine components, and finally obtains traditional Chinese medicine extraction powder rich in traditional Chinese medicine fibers and saponin pharmacological components, wherein the traditional Chinese medicine fibers have the function of increasing and absorbing water in intestinal tracts of diarrhea pets, so that excrement volume is increased and is easier to discharge, and a preliminary effect is generated on intestinal tract conditioning of the pets, and the saponin pharmacological components are difficult to be directly absorbed by organisms for taking effect, therefore, the invention combines the traditional Chinese medicine extraction powder and a self-made probiotic carrier, and the self-made probiotic carrier can stimulate harmful intestinal microorganisms to generate a large amount of glucuronidase, can effectively decompose the saponin pharmacological components, remove glycosyl to generate aglycone to play a pharmacodynamic effect, and the screened probiotic bacterial group can decompose saponin to form monomer metabolites with pharmacological activity, the traditional Chinese medicine aglycone generated by enzymolysis and probiotic population decomposition can inhibit the number of escherichia coli and salmonella in pet intestinal tracts, increase the number of probiotics such as lactobacillus and bifidobacterium and the like, recover the normal structure of intestinal mucosa, increase the length of intestinal villi, reduce the depth of crypts, reduce the diarrhea rate and play a role in conditioning the intestines and stomach of pets;

further, the coating also comprises the following components in parts by weight:

8-12 parts of theanine.

The catechin is added into the raw materials, is used as a dietary polyphenol substance, can effectively influence the population of microorganisms in the intestinal tract, and can inhibit DNA transferase of harmful floras such as escherichia coli and the like in the intestinal tract, so that the proliferation and metabolism of the harmful floras are inhibited, the distribution of the population of the microorganisms in the intestinal tract of the pet is improved, the number of probiotics is increased, the intestines and stomach of the pet are effectively conditioned, and the dietary polyphenol substance has a wide application prospect.

A preparation method of a composite probiotic pet food for coordinating intestines and stomach comprises the following specific preparation steps:

weighing 10-14 parts of taurine, 28-32 parts of self-made probiotic additive, 13-17 parts of traditional Chinese medicine extract powder, 8-12 parts of theanine, 1-2 parts of vitamin C and 3-7 parts of chelated calcium powder, filling into a stirrer, stirring, mixing and packaging to obtain the composite probiotic pet food for regulating intestines and stomach.

Further, the preparation steps of the self-made probiotic additive are as follows:

(1) weighing corncobs, putting the corncobs into a grinder for grinding, sieving the ground corncobs by a 50-mesh sieve to obtain corncob powder, mixing the obtained corncob powder and a sodium hydroxide solution with the mass fraction of 15% according to the mass ratio of the material liquid of 1:15, putting the mixture into a reaction kettle, heating the mixture to 80-90 ℃, preserving the temperature, stirring and reacting the mixture for 150-160 min to obtain a reactant;

(2) mixing the reactant with an ethanol solution with the mass fraction of 70% according to the equal volume, standing and precipitating for 12-14 h, filtering separated filter residue after precipitation is finished, putting the obtained filter residue into an oven, and drying for 1-2 h at 70-80 ℃ to obtain a self-made probiotic carrier;

(3) according to the weight parts, 5-9 parts of Bacillus belgii powder, 16-20 parts of Lactobacillus reuteri powder, 7-11 parts of enterococcus hirae powder, 10-14 parts of Bifidobacterium powder and 9-13 parts of rhamnosus powder are selected and mixed to obtain composite probiotic powder, and the composite probiotic powder, the self-made probiotic carrier, beef powder and deionized water are mixed and stirred according to the mass ratio of 1:1:2:5 to obtain a mixture, namely the self-made probiotic additive.

Further, the preparation method of the traditional Chinese medicine extract powder comprises the following steps:

weighing platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae according to a mass ratio of 3:2:3, putting the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae into a grinder for grinding, sieving the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae with a 50-mesh sieve to obtain mixed traditional Chinese medicine powder, mixing the mixed traditional Chinese medicine powder, deionized water and diethyl ether according to a mass ratio of 1:5:3, heating to 80-90 ℃, stirring for reaction for 30-40 min, standing for layering to remove an upper diethyl ether layer, mixing a lower water layer and n-butyl alcohol according to a volume ratio of 1:1, stirring for reaction at 90-95 ℃ for 1-2 h to obtain a reaction product, and evaporating the reaction product under reduced pressure to obtain traditional Chinese medicine extract powder.

By the scheme, the invention at least has the following advantages:

(1) the xylo-oligosaccharide in the corn cob is extracted by alkali, the low xylan component in the reactant is precipitated by alcohol precipitation, and then the mixed powder of the corn cob fiber and the corn cob low xylan is obtained by filtration and interception, the mixed powder is rich in the fiber and the low xylan component, has large specific surface area, can contain a large amount of probiotics and becomes a load carrier of the probiotics, so that the survival rate of the probiotics is ensured, in addition, the fiber has a polyhydroxy structure and strong water absorption, and can absorb the water in the intestinal tract of a diarrhea pet, so that the volume of excrement is increased and the excrement is easier to discharge, a primary effect is generated for conditioning the intestinal tract of the pet, more importantly, the low xylan component can effectively inhibit the survival of harmful bacteria escherichia coli in the intestines and stomach, so that the harmful bacteria escherichia coli can generate stress metabolism, and further generate a large amount of glucuronidase to prepare for later-stage plant saponin enzymolysis, the low xylan can inhibit the normal metabolic propagation of harmful microorganisms in intestines and stomach, can be used as a binding receptor of pathogenic bacteria to competitively bind with phytohemagglutinin (or carbohydrate-binding protein) on the surfaces of pathogenic bacteria cells, reduces the binding chance of the pathogenic bacteria and epithelial cells of intestinal mucosa, greatly reduces the pathogenic capability, plays a role in regulating the intestinal tract, can reduce the pH value of the intestinal tract, promotes the mineral solubility to be increased, further increases the absorption and utilization of mineral electrolyte components of diarrhea animals, and further regulates the functions of the intestines and stomach of pets, but has selective proliferation effect on beneficial strains screened by the xylooligosaccharide, because the xylooligosaccharide has special beta-1, 4-glycosidic bonds, cannot be decomposed by endogenous enzymes of the animals, cannot be utilized by the harmful microorganisms, can directly reach the tail end of the small intestine and the large intestine, and can be absorbed and utilized by probiotic strains screened by the invention, promoting the massive proliferation of beneficial bacteria, increasing the secretion of short-chain fatty acid, acetic acid and lactic acid, stimulating the peristalsis of intestinal tracts, changing osmotic pressure, increasing the water content of excrement and preventing the gastrointestinal diseases such as constipation and diarrhea;

(2) the invention removes fat-soluble impurities in the traditional Chinese medicine components by ethyl ether, then uses n-butanol to leach saponin active ingredients in the traditional Chinese medicine components, and finally obtains traditional Chinese medicine extraction powder rich in traditional Chinese medicine fibers and saponin pharmacological components, wherein the traditional Chinese medicine fibers have the function of increasing and absorbing water in intestinal tracts of diarrhea pets, so that excrement volume is increased and is easier to discharge, and a preliminary effect is generated on intestinal tract conditioning of the pets, and the saponin pharmacological components are difficult to be directly absorbed by organisms for taking effect, therefore, the invention combines the traditional Chinese medicine extraction powder and a self-made probiotic carrier, and the self-made probiotic carrier can stimulate harmful intestinal microorganisms to generate a large amount of glucuronidase, can effectively decompose the saponin pharmacological components, remove glycosyl to generate aglycone to play a pharmacodynamic effect, and the screened probiotic bacterial group can decompose saponin to form monomer metabolites with pharmacological activity, the traditional Chinese medicine aglycone generated by enzymolysis and probiotic population decomposition can inhibit the number of escherichia coli and salmonella in pet intestinal tracts, increase the number of probiotics such as lactobacillus and bifidobacterium and the like, recover the normal structure of intestinal mucosa, increase the length of intestinal villi, reduce the depth of crypts, reduce the diarrhea rate and play a role in conditioning the intestines and stomach of pets;

(3) the catechin is added into the raw materials, is used as a dietary polyphenol substance, can effectively influence the population of microorganisms in the intestinal tract, and can inhibit DNA transferase of harmful floras such as escherichia coli and the like in the intestinal tract, so that the proliferation and metabolism of the harmful floras are inhibited, the distribution of the population of the microorganisms in the intestinal tract of the pet is improved, the number of probiotics is increased, the intestines and stomach of the pet are effectively conditioned, and the dietary polyphenol substance has a wide application prospect.

The foregoing is a summary of the present invention, and in order to provide a clear understanding of the technical means of the present invention and to be implemented in accordance with the present specification, the following is a detailed description of the preferred embodiments of the present invention.

Detailed Description

The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

Microbial powder number description: bacillus belgii No. BNCC352076, Lactobacillus reuteri No. ATCC55148, enterococcus hirae No. bio-53160; the number of bifidobacterium is ATCC29521, the number of rhamnose bacillus is CICC 6001;

the viable count index of each raw material is 1.9 multiplied by 10 of Bacillus beilesensis⁹cfu/g, Lactobacillus reuteri 1.3X 10¹⁰cfu/g, enterococcus hirae 1.0X 10⁹cfu/g, Bifidobacterium 2.0X 10¹⁰cfu/g, 1.8X 10 of rhamnosus bacterium⁹cfu/g；

(1) Weighing corncobs, putting the corncobs into a grinder for grinding, sieving the ground corncobs by a 50-mesh sieve to obtain corncob powder, mixing the obtained corncob powder and a sodium hydroxide solution with the mass fraction of 15% according to the mass ratio of the material liquid of 1:15, putting the mixture into a reaction kettle, heating the mixture to 80-90 ℃, preserving the temperature, stirring and reacting the mixture for 150-160 min to obtain a reactant; extracting xylo-oligosaccharide in the corncobs by alkali;

(2) mixing the reactant with an ethanol solution with the mass fraction of 70% according to the equal volume, standing and precipitating for 12-14 h, filtering separated filter residue after precipitation is finished, putting the obtained filter residue into an oven, and drying for 1-2 h at 70-80 ℃ to obtain a self-made probiotic carrier; precipitating a low-xylan component in a reactant by alcohol precipitation, and filtering and intercepting to obtain mixed powder of corncob fiber and the corncob low-xylan, wherein the mixed powder is rich in fiber and the low-xylan component, has a large specific surface area and can contain a large amount of probiotics to become a load carrier of the probiotics, so that the survival rate of the probiotics is ensured, in addition, the fiber is of a polyhydroxy structure and has strong water absorption, and the mixed powder can absorb water in the intestinal tract of a diarrhea pet, so that excrement is enlarged in volume and is easier to discharge, and a primary effect is generated on the intestinal tract conditioning of the pet, more importantly, the low-xylan component can effectively inhibit the survival of harmful bacteria escherichia coli in the intestines and stomach, so that the harmful bacteria escherichia coli can generate stress metabolism, and further a large amount of glucuronidase is generated, the preparation is made for later-stage enzymolysis of plant saponin, and the low-xylan can inhibit the normal metabolic propagation of harmful microorganisms in the intestines and stomach, can be used as a binding receptor of pathogenic bacteria to competitively bind with phytohemagglutinin (or carbohydrate binding protein) on the cell surface of the pathogenic bacteria, reduces the chance of binding the pathogenic bacteria with intestinal mucosa epithelial cells, greatly reduces the pathogenic capability, plays a role in regulating the intestinal tract, simultaneously can also reduce the pH of the intestinal tract, promotes the mineral solubility to be increased, further increases the absorption and utilization of mineral electrolyte components of diarrhea animals, and further regulates the gastrointestinal function of pets, but xylooligosaccharide has selective proliferation effect on the beneficial bacteria screened by the invention, because the xylooligosaccharide has special beta-1, 4-glycosidic bond, can not be decomposed by animal endogenous enzyme, can not be utilized by harmful microorganisms, can directly reach the tail end of the small intestine and the large intestine, can be absorbed and utilized by the probiotic bacteria screened by the invention, promotes the large proliferation of the beneficial bacteria, and increases short chain fatty acid, and the like, The secretion of acetic acid and lactic acid stimulates the peristalsis of intestinal tracts, changes osmotic pressure, increases the water content of excrement and prevents gastrointestinal diseases such as constipation, diarrhea and the like;

(3) according to the weight parts, 5-9 parts of Bacillus belgii powder, 16-20 parts of Lactobacillus reuteri powder, 7-11 parts of enterococcus hirae powder, 10-14 parts of Bifidobacterium powder and 9-13 parts of rhamnosus powder are selected and mixed to obtain composite probiotic powder, and the composite probiotic powder, the self-made probiotic carrier, beef powder and deionized water are mixed and stirred according to the mass ratio of 1:1:2:5 to obtain a mixture, namely the self-made probiotic additive; the self-made probiotic additive has the advantages that the weight ratio of various probiotics is optimal, and only under the optimal ratio, the interspecies competition among the probiotics and the decomposition effect of subsequent glucoside can reach the optimal, so that the optimal conditioning effect of the probiotics is realized;

(4) weighing platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae according to a mass ratio of 3:2:3, putting the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae into a grinder for grinding, sieving the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae by a sieve of 50 meshes to obtain mixed traditional Chinese medicine powder, mixing the mixed traditional Chinese medicine powder, deionized water and diethyl ether according to a mass ratio of 1:5:3, heating to 80-90 ℃, stirring for reaction for 30-40 min, standing for layering to remove an upper diethyl ether layer, mixing a lower water layer and n-butyl alcohol according to a volume ratio of 1:1, stirring for reaction at 90-95 ℃ for 1-2 h to obtain a reaction product, and evaporating the reaction product under reduced pressure to obtain traditional Chinese medicine extract powder; the invention removes fat-soluble impurities in the traditional Chinese medicine components by ethyl ether, then uses n-butanol to leach saponin active ingredients in the traditional Chinese medicine components, and finally obtains traditional Chinese medicine extraction powder rich in traditional Chinese medicine fibers and saponin pharmacological components, wherein the traditional Chinese medicine fibers have the function of increasing and absorbing water in intestinal tracts of diarrhea pets, so that excrement volume is increased and is easier to discharge, and a preliminary effect is generated on intestinal tract conditioning of the pets, and the saponin pharmacological components are difficult to be directly absorbed by organisms for taking effect, therefore, the invention combines the traditional Chinese medicine extraction powder and a self-made probiotic carrier, and the self-made probiotic carrier can stimulate harmful intestinal microorganisms to generate a large amount of glucuronidase, can effectively decompose the saponin pharmacological components, remove glycosyl to generate aglycone to play a pharmacodynamic effect, and the screened probiotic bacterial group can decompose saponin to form monomer metabolites with pharmacological activity, the traditional Chinese medicine aglycone generated by enzymolysis and probiotic population decomposition can inhibit the number of escherichia coli and salmonella in pet intestinal tracts, increase the number of probiotics such as lactobacillus and bifidobacterium and the like, recover the normal structure of intestinal mucosa, increase the length of intestinal villi, reduce the depth of crypts, reduce the diarrhea rate and play a role in conditioning the intestines and stomach of pets;

(5) weighing 10-14 parts of taurine, 28-32 parts of self-made probiotic additive, 13-17 parts of traditional Chinese medicine extract powder, 8-12 parts of theanine, 1-2 parts of vitamin C and 3-7 parts of chelated calcium powder, filling into a stirrer, stirring, mixing and packaging to obtain the composite probiotic pet food for regulating intestines and stomach. The catechin is added into the raw materials, is used as a dietary polyphenol substance, can effectively influence the population of microorganisms in the intestinal tract, and can inhibit DNA transferase of harmful floras such as escherichia coli and the like in the intestinal tract, so that the proliferation and metabolism of the harmful floras are inhibited, the distribution of the population of the microorganisms in the intestinal tract of the pet is improved, the number of probiotics is increased, the intestines and stomach of the pet are effectively conditioned, and the dietary polyphenol substance has a wide application prospect.

Example 1

Weighing corncobs, putting the corncobs into a grinder for grinding, sieving the ground corncobs by a 50-mesh sieve to obtain corncob powder, mixing the obtained corncob powder and a sodium hydroxide solution with the mass fraction of 15% according to the mass ratio of the material liquid of 1:15, putting the mixture into a reaction kettle, heating the mixture to 85 ℃, preserving the temperature, stirring and reacting the mixture for 155min to obtain a reactant;

mixing the reactant and 70% ethanol solution according to the equal volume, standing and precipitating for 13h, filtering and separating filter residue after precipitation is finished, putting the obtained filter residue into an oven, and drying for 2h at 75 ℃ to obtain the self-made probiotic carrier;

according to the weight parts, 5 parts of Bacillus belgii powder, 16 parts of Lactobacillus reuteri powder, 7 parts of enterococcus hirae powder, 10 parts of Bifidobacterium powder and 9 parts of rhamnosus bacterium powder are selected and mixed to obtain composite probiotic powder, and the composite probiotic powder, the self-made probiotic carrier, beef powder and deionized water are mixed and stirred according to the mass ratio of 1:1:2:5 to obtain a mixture, namely the self-made probiotic additive;

weighing platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae according to a mass ratio of 3:2:3, putting the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae into a grinder for grinding, sieving the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae by a sieve of 50 meshes to obtain mixed traditional Chinese medicine powder, mixing the mixed traditional Chinese medicine powder, deionized water and diethyl ether according to a mass ratio of 1:5:3, heating to 85 ℃, stirring for reaction for 35min, standing for layering to remove an upper diethyl ether layer, mixing a lower water layer and n-butyl alcohol according to a volume ratio of 1:1, stirring for reaction for 2h at 93 ℃ to obtain a reaction product, and evaporating the reaction product under reduced pressure to obtain traditional Chinese medicine extract powder;

weighing 12 parts of taurine, 30 parts of self-made probiotic additive, 15 parts of traditional Chinese medicine extract powder, 10 parts of theanine, 1 part of vitamin C and 5 parts of chelated calcium powder, filling the materials into a stirrer, stirring, mixing and packaging to obtain the composite probiotic pet food for regulating intestines and stomach.

Example 2

Weighing corncobs, putting the corncobs into a grinder for grinding, sieving the ground corncobs by a 50-mesh sieve to obtain corncob powder, mixing the obtained corncob powder and a sodium hydroxide solution with the mass fraction of 15% according to the mass ratio of the material liquid of 1:15, putting the mixture into a reaction kettle, heating the mixture to 85 ℃, preserving the temperature, stirring and reacting the mixture for 155min to obtain a reactant;

mixing the reactant and 70% ethanol solution according to the equal volume, standing and precipitating for 13h, filtering and separating filter residue after precipitation is finished, putting the obtained filter residue into an oven, and drying for 2h at 75 ℃ to obtain the self-made probiotic carrier;

selecting 6 parts of Bacillus beilis powder, 17 parts of Lactobacillus reuteri powder, 8 parts of enterococcus hirae powder, 11 parts of Bifidobacterium powder and 10 parts of rhamnose bacillus powder, mixing to obtain composite probiotic powder, and mixing and stirring the composite probiotic powder, the self-made probiotic carrier, the beef powder and deionized water according to a mass ratio of 1:1:2:5 to obtain a mixture, namely the self-made probiotic additive;

weighing platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae according to a mass ratio of 3:2:3, putting the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae into a grinder for grinding, sieving the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae by a sieve of 50 meshes to obtain mixed traditional Chinese medicine powder, mixing the mixed traditional Chinese medicine powder, deionized water and diethyl ether according to a mass ratio of 1:5:3, heating to 85 ℃, stirring for reaction for 35min, standing for layering to remove an upper diethyl ether layer, mixing a lower water layer and n-butyl alcohol according to a volume ratio of 1:1, stirring for reaction for 2h at 93 ℃ to obtain a reaction product, and evaporating the reaction product under reduced pressure to obtain traditional Chinese medicine extract powder;

weighing 12 parts of taurine, 30 parts of self-made probiotic additive, 15 parts of traditional Chinese medicine extract powder, 10 parts of theanine, 1 part of vitamin C and 5 parts of chelated calcium powder, filling the materials into a stirrer, stirring, mixing and packaging to obtain the composite probiotic pet food for regulating intestines and stomach.

Example 3

Weighing corncobs, putting the corncobs into a grinder for grinding, sieving the ground corncobs by a 50-mesh sieve to obtain corncob powder, mixing the obtained corncob powder and a sodium hydroxide solution with the mass fraction of 15% according to the mass ratio of the material liquid of 1:15, putting the mixture into a reaction kettle, heating the mixture to 85 ℃, preserving the temperature, stirring and reacting the mixture for 155min to obtain a reactant;

mixing the reactant and 70% ethanol solution according to the equal volume, standing and precipitating for 13h, filtering and separating filter residue after precipitation is finished, putting the obtained filter residue into an oven, and drying for 2h at 75 ℃ to obtain the self-made probiotic carrier;

according to the weight parts, 7 parts of Bacillus beiLeisi powder, 18 parts of Lactobacillus reuteri powder, 9 parts of enterococcus hirae powder, 12 parts of bifidobacterium powder and 11 parts of rhamnosus bacterium powder are selected and mixed to obtain composite probiotic powder, and the composite probiotic powder, the self-made probiotic carrier, the beef powder and deionized water are mixed and stirred according to the mass ratio of 1:1:2:5 to obtain a mixture, namely the self-made probiotic additive;

weighing platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae according to a mass ratio of 3:2:3, putting the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae into a grinder for grinding, sieving the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae by a sieve of 50 meshes to obtain mixed traditional Chinese medicine powder, mixing the mixed traditional Chinese medicine powder, deionized water and diethyl ether according to a mass ratio of 1:5:3, heating to 85 ℃, stirring for reaction for 35min, standing for layering to remove an upper diethyl ether layer, mixing a lower water layer and n-butyl alcohol according to a volume ratio of 1:1, stirring for reaction for 2h at 93 ℃ to obtain a reaction product, and evaporating the reaction product under reduced pressure to obtain traditional Chinese medicine extract powder;

weighing 12 parts of taurine, 30 parts of self-made probiotic additive, 15 parts of traditional Chinese medicine extract powder, 10 parts of theanine, 1 part of vitamin C and 5 parts of chelated calcium powder, filling the materials into a stirrer, stirring, mixing and packaging to obtain the composite probiotic pet food for regulating intestines and stomach.

Example 4

Weighing corncobs, putting the corncobs into a grinder for grinding, sieving the ground corncobs by a 50-mesh sieve to obtain corncob powder, mixing the obtained corncob powder and a sodium hydroxide solution with the mass fraction of 15% according to the mass ratio of the material liquid of 1:15, putting the mixture into a reaction kettle, heating the mixture to 85 ℃, preserving the temperature, stirring and reacting the mixture for 155min to obtain a reactant;

mixing the reactant and 70% ethanol solution according to the equal volume, standing and precipitating for 13h, filtering and separating filter residue after precipitation is finished, putting the obtained filter residue into an oven, and drying for 2h at 75 ℃ to obtain the self-made probiotic carrier;

according to the weight parts, 8 parts of Bacillus beiLeisi powder, 19 parts of Lactobacillus reuteri powder, 10 parts of enterococcus hirae powder, 13 parts of bifidobacterium powder and 12 parts of rhamnosus bacterium powder are selected and mixed to obtain composite probiotic powder, and the composite probiotic powder, the self-made probiotic carrier, the beef powder and deionized water are mixed and stirred according to the mass ratio of 1:1:2:5 to obtain a mixture, namely the self-made probiotic additive;

weighing platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae according to a mass ratio of 3:2:3, putting the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae into a grinder for grinding, sieving the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae by a sieve of 50 meshes to obtain mixed traditional Chinese medicine powder, mixing the mixed traditional Chinese medicine powder, deionized water and diethyl ether according to a mass ratio of 1:5:3, heating to 85 ℃, stirring for reaction for 35min, standing for layering to remove an upper diethyl ether layer, mixing a lower water layer and n-butyl alcohol according to a volume ratio of 1:1, stirring for reaction for 2h at 93 ℃ to obtain a reaction product, and evaporating the reaction product under reduced pressure to obtain traditional Chinese medicine extract powder;

weighing 12 parts of taurine, 30 parts of self-made probiotic additive, 15 parts of traditional Chinese medicine extract powder, 10 parts of theanine, 1 part of vitamin C and 5 parts of chelated calcium powder, filling the materials into a stirrer, stirring, mixing and packaging to obtain the composite probiotic pet food for regulating intestines and stomach.

Example 5

Weighing corncobs, putting the corncobs into a grinder for grinding, sieving the ground corncobs by a 50-mesh sieve to obtain corncob powder, mixing the obtained corncob powder and a sodium hydroxide solution with the mass fraction of 15% according to the mass ratio of the material liquid of 1:15, putting the mixture into a reaction kettle, heating the mixture to 85 ℃, preserving the temperature, stirring and reacting the mixture for 155min to obtain a reactant;

mixing the reactant and 70% ethanol solution according to the equal volume, standing and precipitating for 13h, filtering and separating filter residue after precipitation is finished, putting the obtained filter residue into an oven, and drying for 2h at 75 ℃ to obtain the self-made probiotic carrier;

according to the weight parts, 9 parts of Bacillus beiLeisi powder, 20 parts of Lactobacillus reuteri powder, 11 parts of enterococcus hirae powder, 14 parts of bifidobacterium powder and 13 parts of rhamnosus bacterium powder are selected and mixed to obtain composite probiotic powder, and the composite probiotic powder, the self-made probiotic carrier, the beef powder and deionized water are mixed and stirred according to the mass ratio of 1:1:2:5 to obtain a mixture, namely the self-made probiotic additive;

weighing platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae according to a mass ratio of 3:2:3, putting the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae into a grinder for grinding, sieving the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae by a sieve of 50 meshes to obtain mixed traditional Chinese medicine powder, mixing the mixed traditional Chinese medicine powder, deionized water and diethyl ether according to a mass ratio of 1:5:3, heating to 85 ℃, stirring for reaction for 35min, standing for layering to remove an upper diethyl ether layer, mixing a lower water layer and n-butyl alcohol according to a volume ratio of 1:1, stirring for reaction for 2h at 93 ℃ to obtain a reaction product, and evaporating the reaction product under reduced pressure to obtain traditional Chinese medicine extract powder;

weighing 12 parts of taurine, 30 parts of self-made probiotic additive, 15 parts of traditional Chinese medicine extract powder, 10 parts of theanine, 1 part of vitamin C and 5 parts of chelated calcium powder, filling the materials into a stirrer, stirring, mixing and packaging to obtain the composite probiotic pet food for regulating intestines and stomach.

Performance test

The performance test tests were performed on examples 1 to 5, and the test results are shown in tables 1 and 2:

detection method/test method

The method for detecting the effect of conditioning the intestines and stomach comprises the following steps:

selecting 50 moderate diarrhea pet dogs from a pet hospital, averagely dividing the pet dogs into two groups, wherein each group comprises 25 pet dogs, one group is used as an experimental group, the other group is used as a control group, the pet dogs of the experimental group are fed with the common feed by adding 10g of the composite probiotic pet food for regulating intestines and stomach of the invention into the pet feed every day, so as to obtain the regulated feed, the pet dogs of the experimental group are fed with the common feed, the feeding amount of the normal feed of the control group is the same as that of the experimental group, the diarrhea rates of the pets after feeding for 5 days, 10 days, 15 days, 30 days, 45 days and 60 days are respectively observed, and the lower the diarrhea rate is, the better the effect of regulating the intestines and stomach is;

detection test of species of microorganisms in intestines and stomach of pets:

respectively collecting pet feces of an experimental group and a control group after feeding for 60 days, culturing for 18-24 h at 37 ℃ by using a slant culture medium, simultaneously performing common bacterial culture and anaerobic bacterial culture, observing bacterial colonies in the culture medium, measuring the diameters of different bacterial colonies by using a vernier caliper, and detecting the species of the bacterial colonies so as to judge various bacteria and contents thereof in intestines and stomachs;

TABLE 1 gastrointestinal conditioning effect test results

As can be seen from the test data in the table, the diarrhea of the pet dogs in the examples 1-5 of the application is obviously reduced after the food of the invention is fed, the diarrhea number of the pet dogs serving as the control group is not obviously changed, so that the composite probiotic pet food for conditioning intestines and stomach has the effect of conditioning the intestinal tract function of the pet, and in the examples 1-5, wherein the number of diarrhea pet dogs in examples 1-3 gradually decreased, and the number of diarrhea increased from example 4, the self-made probiotic additive is characterized in that the self-made probiotic additive comprises probiotics and glucoside, wherein the probiotics are mixed with the glucoside, and the mixture ratio of the probiotics to the glucoside is optimized.

TABLE 2 detection results of species and content of microorganisms in pet intestines and stomach

As can be seen from the detection data in the above table, the colony diameter of the beneficial bacteria in the examples 1-5 of the invention is significantly higher than that of the beneficial bacteria in the comparative example, the colony diameter of the harmful bacteria in the example is obviously lower than that of the comparative example, so that the composite probiotic pet food for coordinating intestines and stomach of the invention can obviously improve the species and the quantity of the probiotics in the intestines and the stomach of the pet dog, and the beneficial bacteria in the example 3 are most abundant in variety and quantity and the quantity of the harmful bacteria is least, so that the self-made beneficial bacteria additive provided by the invention has the best weight ratio, and only under the best weight ratio, the interspecies competition among the beneficial bacteria and the decomposition effect of subsequent glucoside can reach the best, the best conditioning effect of the beneficial bacteria is realized, and meanwhile, the technical scheme provided by the invention is feasible.

Comparative example

Comparative example 1: the diarrhea rate of the pet dog fed with the composite probiotic pet food for regulating intestines and stomach is in a descending trend, the descending degree is obviously superior to that of a control group without the composite probiotic food, and the descending degree of the diarrhea of the pet dog is larger than that of the pet dog which is fed with the composite probiotic food for regulating intestines and stomach and is singly used in the composite probiotic powder, mixed use of any 2 bactericides, mixed use of any 3 bactericides and mixed use of any 4 bactericides. Therefore, the intestinal microbial population and the number of the intestinal microbial population of the pet dog can be improved to the maximum extent only when all the strains exist and are matched for use in the composite probiotic powder;

comparative example 2

In comparative example 2, the self-made probiotic carrier of the invention was not added, and other conditions and component ratios were the same as in example 1;

comparative example 3

In comparative example 3, the Chinese medicine extract powder of the present invention was not added, and other conditions and component ratios were the same as those in example 1;

comparative example 4

In comparative example 4, the catechins of the present invention were not added, and the other conditions and component ratios were the same as in example 1;

respectively carrying out performance tests on the composite probiotic pet food prepared in the comparative examples 2-4, wherein the test results are shown in tables 3 and 4;

detection method/test method

The method for detecting the effect of conditioning the intestines and stomach comprises the following steps:

selecting 50 moderate diarrhea pet dogs from a pet hospital, averagely dividing the pet dogs into two groups, wherein each group comprises 25 pet dogs, one group is used as an experimental group, the other group is used as a control group, the pet dogs of the experimental group are fed with the common feed by adding 10g of the composite probiotic pet food for regulating intestines and stomach of the invention into the pet feed every day, so as to obtain the regulated feed, the pet dogs of the experimental group are fed with the common feed, the feeding amount of the normal feed of the control group is the same as that of the experimental group, the diarrhea rates of the pets after feeding for 5 days, 10 days, 15 days, 30 days, 45 days and 60 days are respectively observed, and the lower the diarrhea rate is, the better the effect of regulating the intestines and stomach is;

detection test of species of microorganisms in intestines and stomach of pets:

respectively collecting pet feces of an experimental group and a control group after feeding for 60 days, culturing for 18-24 h at 37 ℃ by using a slant culture medium, simultaneously performing common bacterial culture and anaerobic bacterial culture, observing bacterial colonies in the culture medium, measuring the diameters of different bacterial colonies by using a vernier caliper, and detecting the species of the bacterial colonies so as to judge various bacteria and contents thereof in intestines and stomachs;

TABLE 3 gastrointestinal conditioning effect test results

TABLE 4 detection results of species and content of microorganisms in pet intestines and stomach

As can be seen from the test data in tables 3 and 4, the comparative example 2 has the same conditions and component ratios as in example 1 due to the absence of the home-made probiotic carrier of the present invention; finally, the diarrhea treatment effect on the pet dog is obviously reduced, the quantity of beneficial bacteria in the intestinal tract of the pet dog is reduced, and the quantity of harmful bacteria is increased, so that the method can be proved that xylo-oligosaccharide in the corn cob is extracted by alkali, the low-xylan component in the reactant is precipitated by alcohol precipitation, and then the mixed powder of the corn cob fiber and the corn cob low-xylan is obtained by filtration and interception, the mixed powder is rich in fiber and the low-xylan component, has large specific surface area, can contain a large amount of probiotic bacteria and become a load carrier thereof, the survival rate of the probiotic bacteria is ensured, in addition, the fiber is of a polyhydroxy structure, has strong water absorption, can absorb the water in the intestinal tract of the diarrhea pet, increases the volume of excrement and is easier to discharge, and has a primary effect on conditioning the intestinal tract of the pet, more importantly, the low-xylan component in the intestinal tract can effectively inhibit the survival of harmful bacteria escherichia coli in the intestines and stomach, so that the strain can generate stress metabolism, and further generate a large amount of glucuronidase, and prepare for later-stage enzymolysis of plant saponin, the low-xylan can inhibit normal metabolic propagation of harmful microorganisms in intestines and stomach, can be used as a binding receptor of pathogenic bacteria to competitively bind with phytohemagglutinin (or carbohydrate binding protein) on the cell surface of the pathogenic bacteria, reduce the chance of binding of the pathogenic bacteria and epithelial cells of intestinal mucosa, greatly reduce pathogenic capability, play a role in regulating intestinal tract, reduce the pH value of the intestinal tract, promote the increase of mineral solubility, further increase the absorption and utilization of mineral electrolyte components of diarrhea animals, and further regulate the functions of intestines and stomach of pets, but the xylooligosaccharide has selective proliferation effect on the screened beneficial strains of the invention, because the xylooligosaccharide has special beta-1, 4-glycosidic bond and can not be decomposed by endogenous enzymes of animals, the probiotic bacteria can not be utilized by harmful microorganisms, can directly reach the tail end of the small intestine and the large intestine, and are absorbed and utilized by the probiotic bacteria screened by the invention, so that the massive proliferation of beneficial bacteria is promoted, the secretion of short-chain fatty acid, acetic acid and lactic acid is increased, the peristalsis of the intestinal tract is stimulated, the osmotic pressure is changed, the water content of excrement is increased, and the generation of gastrointestinal diseases such as constipation, diarrhea and the like is prevented;

in addition, in the comparative example 3, as the traditional Chinese medicine extraction powder is not added, and other conditions and component proportions are the same as those in the example 1, the treatment effect of diarrhea of the pet dog is obviously reduced, the number of beneficial bacteria in intestinal tracts of the pet dog is reduced, and the number of harmful bacteria is increased, so that the method can be proved that fat-soluble impurities in the traditional Chinese medicine components are removed by ethyl ether, and saponin effective components in the traditional Chinese medicine components are leached by n-butyl alcohol, and the traditional Chinese medicine extraction powder rich in traditional Chinese medicine fibers and saponin pharmacological components is finally obtained, wherein the traditional Chinese medicine fibers have the function of increasing the absorption of water in the intestinal tracts of diarrhea pets, so that the excrement volume is increased and is easier to discharge, and the traditional Chinese medicine extraction powder has a primary effect on conditioning the intestinal tracts of the pets, and the saponin pharmacological components are difficult to be directly absorbed by organisms, so the traditional Chinese medicine extraction powder and the self-made probiotic carrier are compounded and used together, because the self-made probiotic carrier can stimulate harmful intestinal microorganisms to generate a large amount of glucuronidase, saponin pharmacological components can be effectively decomposed, glycosyl of the saponin is removed to generate aglycone, so that the drug effect is achieved, and the screened probiotic bacterial population can also decompose saponin to form monomer metabolite aglycone with pharmacological activity;

also, comparative example 4 was the same as in example 1 in terms of other conditions and component ratios since no catechin of the present invention was added; the diarrhea treatment effect of the pet dog is obviously reduced, the quantity of beneficial bacteria in the intestinal tract of the pet dog is reduced, and the quantity of harmful bacteria is increased, so that the fact that catechin is added in the raw materials can be confirmed, the catechin serves as a dietary polyphenol substance and can effectively influence the population of microorganisms in the intestinal tract, the catechin can inhibit DNA transferase of harmful bacteria such as escherichia coli in the intestinal tract, the proliferation and metabolism of the harmful bacteria are inhibited, the distribution of the population of the microorganisms in the intestinal tract of the pet is improved, the quantity of the probiotic is increased, the intestines and the stomach of the pet are effectively conditioned, and the application prospect is wide.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, it should be noted that, for those skilled in the art, many modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.

Claims (4)

1. A composite probiotic pet food for coordinating intestines and stomach is characterized in that: the composite material comprises the following raw materials in parts by weight:

10-14 parts of taurine;

28-32 parts of a self-made probiotic additive;

1-2 parts of vitamin C;

3-7 parts of chelated calcium powder;

13-17 parts of traditional Chinese medicine extraction powder;

8-12 parts of theanine;

the Chinese medicinal extract powder is prepared by reacting radix Platycodi, cortex et radix Polygalae and rhizoma anemarrhenae, deionized water, diethyl ether and n-butanol;

the preparation steps of the self-made probiotic additive are as follows:

(1) weighing corncobs, putting the corncobs into a grinder for grinding, sieving the ground corncobs by a 50-mesh sieve to obtain corncob powder, mixing the obtained corncob powder and a sodium hydroxide solution with the mass fraction of 15% according to the mass ratio of the material liquid of 1:15, putting the mixture into a reaction kettle, heating the mixture to 80-90 ℃, preserving the temperature, stirring and reacting the mixture for 150-160 min to obtain a reactant;

(2) mixing the reactant with an ethanol solution with the mass fraction of 70% according to the equal volume, standing and precipitating for 12-14 h, filtering separated filter residue after precipitation is finished, putting the obtained filter residue into an oven, and drying for 1-2 h at 70-80 ℃ to obtain a self-made probiotic carrier;

(3) according to the weight parts, 5-9 parts of Bacillus belgii powder, 16-20 parts of Lactobacillus reuteri powder, 7-11 parts of enterococcus hirae powder, 10-14 parts of Bifidobacterium powder and 9-13 parts of rhamnosus powder are selected and mixed to obtain composite probiotic powder, and the composite probiotic powder, the self-made probiotic carrier, beef powder and deionized water are mixed and stirred according to the mass ratio of 1:1:2:5 to obtain a mixture, namely the self-made probiotic additive.

2. The composite probiotic pet food for conditioning intestines and stomach according to claim 1, characterized in that:

the composite probiotic powder comprises the following raw materials in parts by weight:

7 parts of Bacillus belgii powder;

18 parts of lactobacillus reuteri powder;

9 parts of enterococcus hirae powder;

12 parts of bifidobacterium powder;

and mixing 11 parts of rhamnose bacillus powder to obtain the composite probiotic powder.

3. The method for preparing the composite probiotic pet food for conditioning intestines and stomach according to claim 1 is characterized in that: the preparation method comprises the following specific steps:

weighing 10-14 parts of taurine, 28-32 parts of self-made probiotic additive, 13-17 parts of traditional Chinese medicine extract powder, 8-12 parts of theanine, 1-2 parts of vitamin C and 3-7 parts of chelated calcium powder, filling into a stirrer, stirring, mixing and packaging to obtain the composite probiotic pet food for regulating intestines and stomach.

4. The method for preparing the composite probiotic pet food for conditioning intestines and stomach according to claim 3 is characterized in that: the preparation method of the traditional Chinese medicine extract powder comprises the following steps:

weighing platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae according to a mass ratio of 3:2:3, putting the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae into a grinder for grinding, sieving the platycodon grandiflorum, polygala tenuifolia and rhizoma anemarrhenae with a 50-mesh sieve to obtain mixed traditional Chinese medicine powder, mixing the mixed traditional Chinese medicine powder, deionized water and diethyl ether according to a mass ratio of 1:5:3, heating to 80-90 ℃, stirring for reaction for 30-40 min, standing for layering to remove an upper diethyl ether layer, mixing a lower water layer and n-butyl alcohol according to a volume ratio of 1:1, stirring for reaction at 90-95 ℃ for 1-2 h to obtain a reaction product, and evaporating the reaction product under reduced pressure to obtain traditional Chinese medicine extract powder.