CN110476984A - Application of the high-hydroscopicity bamboo fibre polysaccharide in preparation mouse intestinal obstruction agent - Google Patents
Application of the high-hydroscopicity bamboo fibre polysaccharide in preparation mouse intestinal obstruction agent Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention belongs to plague of rats defense technique field, a kind of application of high-hydroscopicity bamboo fibre polysaccharide in preparation mouse intestinal obstruction agent is specifically disclosed.The present invention also provides a kind of mouse intestinal obstruction agent, are prepared from the following raw materials according to parts by weight: 5-10 parts of high-hydroscopicity bamboo fibre polysaccharide, conventional mouse eat 5-10 parts, 2-3 parts of essence.Mouse intestinal obstruction agent provided by the invention, preparation process is simple and effective, raw material is easier to obtain, it can make its intestinal occlusion after muroid is edible and dead, have the advantages that quick, palatability is good, no pollution to the environment, and high-hydroscopicity bamboo fibre polysaccharide has good water suction, moisture holding capacity, can quickly absorb water after mouse feeding promote mouse stomach and intestine expand or stomach in food solid state, and it is formed and is blocked in enteron aisle, mouse death due to gastrointestinal disturbances absorption function repays mistake, is a kind of environment-friendlyrodenticide rodenticide of novel prevention and treatment plague of rats.
Description
Technical field
The present invention relates to plague of rats defense technique fields, and in particular to a kind of high-hydroscopicity bamboo fibre polysaccharide is in preparation mouse intestines
Block the application in agent.
Background technique
Mouse type in the world is miscellaneous, and quantity is more, and distribution is wide, and reproduction speed is fast, not only to building, crops, forest
Equal generations harm, also carries and infects a variety of viruses, be the plague, rabies, encephalitis, the false tuberculosis of brucellosis and yellow subcutaneous ulcer
A variety of epiphytotics natural reservoir (of bird flu viruses) such as disease.The plague of rats influences people's life, destroys ecological environment, causing to national economy can not estimate
The loss of amount.In recent years since the abuse of chemicals, muroid drug resistance enhance, the trend seriously aggravated is presented in the plague of rats, has become
For one of world's biologic disaster problem.
Chemical rodenticide is present most important rat-bane, quick, and mouse uses dead immediately, master after such drug
Wanting function is effectively to kill mouse or destroy its fecundity, but chemical rodenticide pollutes environment, easily produces to people, animal
Raw secondary murder by poisoning, and muroid can be made to generate resistance, secondly do not have specificity, harm is generated to Natural Enemies of Rats, more makes the plague of rats
Aggravation；And since mouse is prone to supicion, existence IQ is higher, because of its chemical rodenticide acute the characteristics of killing mouse, easily makes other
Mouse throws doubt upon, and leads to the efficiency reduction that kills mouse.Therefore develop it is nontoxic, it is free from environmental pollution, do not develop drug resistance, use peace
Complete novel rat-bane at a slow speed has become problem to be solved.
It is the more few for traditional rat-bane, the either medicament residue outside mouse body or the intracorporal medicament residue of mouse
Certain harm can be generated to plant or other secondary animals less, and high-hydroscopicity bamboo fibre polysaccharide is nontoxic, has powerful
Water suction, moisture holding capacity, soil can also be improved, prevented erosion, it is used for preventing for the plague of rats, good society will be generated
It can benefit and ecological benefits.How suitable medicament is made in high-hydroscopicity bamboo fibre polysaccharide, improves its palatability, it is used for
The prevention and treatment of the plague of rats has become urgent problem to be solved.
Summary of the invention
The problems such as making mouse generate resistance, the low efficiency that kills mouse to solve existing rat-bane easily, the present invention provides once
Technical solution:
First purpose of the invention is to provide a kind of high-hydroscopicity bamboo fibre polysaccharide answering in preparation mouse intestinal obstruction agent
With the high-hydroscopicity bamboo fibre polysaccharide has the molecular structure as shown in formula (I):
Wherein: n is 2000~8000.
The high-hydroscopicity bamboo fibre polysaccharide the preparation method is as follows:
S1, bamboo fibre fine grained 100g, acrylic acid 20g, deionized water 300g, potassium peroxydisulfate are weighed respectively in parts by weight
1g, phenyl bis- (2,4,6- trimethylbenzoyl) phosphine oxide 1g, it is spare；
Wherein, the bamboo fibre fine grained is prepared by the following method to obtain: annual moso bamboo being crushed and crosses 100 mesh
Sieve, obtains bamboo fibre fine grained；
S2, bamboo fibre fine grained weighed in S1 and potassium peroxydisulfate are mixed and is ground, then react 4h, obtain alkalization bamboo
Fiber；
S3, deionized water weighed in S1 is added in alkalization bamboo fibre obtained in S2, is then added in S1 and weighs
Acrylic acid, 20min is stirred to react at 50 DEG C, then keeps the temperature 1h, wash, obtain reaction bamboo fibre；
S4, bis- (2,4, the 6- trimethylbenzoyl) phosphine oxides of phenyl are added into the reaction bamboo fibre that S3 is obtained, then
Sealing, is then used at 45 DEG C60Co- gamma-rays irradiates 2h in the case where irradiation dose is 5kGy, and washing is dried to get macromolecule is arrived
Bamboo fibre polysaccharide.
Second purpose of the invention is to provide a kind of mouse intestinal obstruction agent, and the principle active component in the intestinal obstruction agent is
High-hydroscopicity bamboo fibre polysaccharide.
Preferably, the intestinal obstruction agent is made of the following components according to parts by weight: high-hydroscopicity bamboo fibre polysaccharide 5-10
Part, conventional mouse eat 5-10 parts, 2-3 parts of auxiliary material.
It is further preferred that the intestinal obstruction agent is prepared from the following raw materials according to parts by weight: high-hydroscopicity bamboo is fine
Tie up 8 parts of polysaccharide, conventional mouse eats 6 parts, 2 parts of auxiliary material.
Preferably, the conventional mouse food is according to parts by weight by 15-20 portions of maize flours, 13-22 parts of dregs of beans, 3-5 portions of sesame oil
And 3-5 portions of peanuts add 10 parts of water dry after mixing be made.
Preferably, the auxiliary material by following parts by weight material composition: 4-10 parts of essence, 2-3 parts of salt.
It is further preferred that the essence is one of beef flavor, carrot essence or peanut essence.
The present invention also provides a kind of preparation methods of mouse intestinal obstruction agent, specifically includes the following steps:
Step 1 weighs 15-20 portions of maize flours, 13-22 parts of dregs of beans, 3-5 portions of sesame oil and 3-5 parts of peanuts, 10 in parts by weight
Part water, conventional mouse food is made in drying after mixing, spare；
Step 2 weighs 4-10 parts of essence, 2-3 parts of salt after mixing in parts by weight, and addition is equivalent to its total volume 2
Water again dissolves it sufficiently, obtains auxiliary material, spare；
Step 3 weighs 5-10 parts of high-hydroscopicity bamboo fibre polysaccharide, the conventional mouse food of gained in step 1 in parts by weight
2-3 parts of auxiliary material of gained, spare in 5-10 parts and step 2；
The high-hydroscopicity bamboo fibre polysaccharide weighed in step 3 is mixed well with the conventional mouse food and is made by step 4
Mixture A, for use；
The auxiliary material weighed in step 3 is uniformly sprayed on the mixture A by step 5, obtains mixture B；
Step 6 will obtain mixture B and place 24-48h under the conditions of 40-80 DEG C to get the intestinal obstruction agent in step 5.
The prior art is compared, the invention has the benefit that
1, mouse intestinal obstruction agent provided by the invention, preparation process is easy, and quality standard is easily controllable, and the finished product of preparation is suitable
Mouth property is good；Material therefor is degradable, using physical action change the functions of intestines and stomach prevention and control plague of rats, it is nontoxic to mouse, to environment without
Poison is harmless, pollution-free；It is at low cost, application easy to spread；
2, the high-hydroscopicity bamboo fibre polysaccharide in the present invention is a kind of high molecular material that absorbent function is especially strong, nontoxic nothing
Evil, water suction can be drawn since bamboo fibre molecular surface has a large amount of hydrophilic radical hydroxyl, carboxyl and a large amount of hole
Moisture in stomach of rats and enteron aisle makes the mouse after eating generate thirsty sense, and then continues to drink water, finally expanded because stomach absorbs water,
Intestinal occlusion and it is dead, harmless to other secondary animals, free from environmental pollution, use is safe, and muroid does not develop drug resistance.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range.
Fig. 1 is that anatomical contrast schemes after eating common mouse food and the mice dying of mouse intestinal obstruction agent；
Fig. 2 is to eat stomach comparison diagram after common mouse food and the mice dying of mouse intestinal obstruction agent；
Fig. 3 is that stomach anatomical contrast schemes after eating common mouse food and the mice dying of mouse intestinal obstruction agent；
Fig. 4 is that large intestine anatomical contrast schemes after eating common mouse food and the mice dying of mouse intestinal obstruction agent；
Fig. 5 is that anus anatomical contrast schemes after eating common mouse food and the mice dying of mouse intestinal obstruction agent；
Fig. 6 is to eat common mouse food and anatomical contrast after the LE mouse death of mouse intestinal obstruction agent to scheme；
Fig. 7 is to eat common mouse food and stomach comparison diagram after the LE mouse death of mouse intestinal obstruction agent；
Fig. 8 is to eat common mouse food and stomach anatomical contrast after the LE mouse death of mouse intestinal obstruction agent to scheme；
Fig. 9 is to eat common mouse food and large intestine anatomical contrast after the LE mouse death of mouse intestinal obstruction agent to scheme；
Figure 10 is to eat common mouse food and anus comparison diagram after the LE mouse death of mouse intestinal obstruction agent；
Figure 11 is to eat common mouse food and anus anatomical contrast after the LE mouse death of mouse intestinal obstruction agent to scheme；
Wherein, left figure is control group in Fig. 1-11, and right figure is experimental group.
Specific embodiment
It can be further clearly understood from the present invention by example given below and experimental example, but following the description is not
It is limitation of the invention.For those skilled in the art according to made by foregoing invention content it is some it is nonessential improvement with
Adjustment, will also be within the scope of the present invention.
The present invention provides a kind of application of high-hydroscopicity bamboo fibre polysaccharide in preparation mouse intestinal obstruction agent, for the plague of rats
Prevention and treatment, having the function of reaching by control in rodent pests population quantity reduces muroid harm；
High-hydroscopicity bamboo fibre polysaccharide has the molecular structure as shown in formula (I):
Wherein: n is 2000~8000；
High-hydroscopicity bamboo fibre polysaccharide is a kind of high molecular material that absorbent function is especially strong, nontoxic, and mouse is edible
The moisture in stomach of rats and enteron aisle can be drawn afterwards, so that the mouse after eating is generated thirsty sense, and then continue to drink water, finally because of stomach
Water suction expand, intestinal occlusion and it is dead, harmless to other secondary animals, free from environmental pollution, use is safe, and muroid does not generate anti-medicine
Property.
It is since high-hydroscopicity bamboo fibre polysaccharide is tasteless, high-hydroscopicity bamboo fibre polysaccharide and other non-active ingredients is (as usual
Rule mouse food, essence) it is used to prepare mouse intestinal obstruction agent together, the grazing rate of mouse can be increased, to increase deratization rate.Specifically
Implementation method is as follows:
Embodiment 1
A kind of mouse intestinal obstruction agent, is made of following raw material according to parts by weight: 5 parts of high-hydroscopicity bamboo fibre polysaccharide,
Conventional mouse eats 5 parts, 2 parts of auxiliary material；
High-hydroscopicity bamboo fibre polysaccharide has the structure as shown in formula (I):
Wherein, conventional mouse food is in parts by weight by 15 portions of maize flours, 13 parts of dregs of beans, 3 portions of sesame oil and 3 parts of peanut systems
；
Auxiliary material is made by 4 parts of beef flavors and 2 portions of salt in parts by weight.
Mouse intestinal obstruction agent specific the preparation method comprises the following steps:
Step 1 weighs 15 portions of maize flours, 13 parts of dregs of beans, 3 portions of sesame oil and 3 portions of peanuts in parts by weight, adds 10 parts of water mixing
Conventional mouse food is made in drying after uniformly, spare；
Step 2 weighs 4 parts of beef flavors and 2 portions of salt after mixing in parts by weight, and addition is equivalent to its total volume 2
Water again dissolves it sufficiently, obtains auxiliary material, spare；
Step 3, weigh 5 parts of high-hydroscopicity bamboo fibre polysaccharide in parts by weight, conventional mouse eats institute in 10 parts and step 2
Must be 3 parts of auxiliary material, it is spare；
The high-hydroscopicity bamboo fibre polysaccharide weighed in step 3 is mixed well with the conventional mouse food and is made by step 4
Mixture A, for use；
The auxiliary material weighed in step 3 is added in hand pressure sprayer step 5, with hand pressure sprayer by solution
It is uniformly sprayed on mixture A, during which ceaselessly stirs, obtain mixture B, mixture B is pressed into striped composite at this time；
Step 6 will obtain mixture B and place 48h under the conditions of 40 DEG C in step 5, permeate auxiliary material solution slowly
Into striped composite to get the intestinal obstruction agent, normal temperature storage after packed packaging.
Embodiment 2
A kind of mouse intestinal obstruction agent, is made of following raw material according to parts by weight: 8 parts of high-hydroscopicity bamboo fibre polysaccharide,
Conventional mouse eats 6 parts, 2 parts of auxiliary material；
High-hydroscopicity bamboo fibre polysaccharide has the structure as shown in formula (I):
Wherein, conventional mouse food is in parts by weight by 18 portions of maize flours, 18 parts of dregs of beans, 4 portions of sesame oil and 4 parts of peanut systems
；
Auxiliary material is made by 7 parts of beef flavors and 3 portions of salt in parts by weight.
Mouse intestinal obstruction agent specific the preparation method comprises the following steps:
Step 1 weighs 18 portions of maize flours, 18 parts of dregs of beans, 4 portions of sesame oil and 4 portions of peanuts in parts by weight, adds 10 parts of water mixing
Conventional mouse food is made in drying after uniformly, spare；
Step 2 weighs 7 parts of beef flavors and 3 portions of salt after mixing in parts by weight, and addition is equivalent to its total volume 2
Water again dissolves it sufficiently, obtains auxiliary material, spare；
Step 3, weigh 8 parts of high-hydroscopicity bamboo fibre polysaccharide in parts by weight, conventional mouse eats institute in 6 parts and step 2
Must be 2 parts of auxiliary material, it is spare；
The high-hydroscopicity bamboo fibre polysaccharide weighed in step 3 is mixed well with the conventional mouse food and is made by step 4
Mixture A, for use；
The auxiliary material weighed in step 3 is added in hand pressure sprayer step 5, with hand pressure sprayer by solution
It is uniformly sprayed on mixture A, during which ceaselessly stirs, obtain mixture B, mixture B is pressed into striped composite at this time；
Step 6 will obtain mixture B and place 36h under the conditions of 60 DEG C in step 5, permeate auxiliary material solution slowly
Into striped composite to get the intestinal obstruction agent, normal temperature storage after packed packaging.
Embodiment 3
A kind of mouse intestinal obstruction agent, is made of following raw material according to parts by weight: 10 parts of high-hydroscopicity bamboo fibre polysaccharide,
Conventional mouse eats 10 parts, 3 parts of auxiliary material；
High-hydroscopicity bamboo fibre polysaccharide has the structure as shown in formula (I):
Wherein, conventional mouse food is in parts by weight by 20 portions of maize flours, 22 parts of dregs of beans, 5 portions of sesame oil and 5 parts of peanut systems
；
Auxiliary material is made by 10 parts of beef flavors and 3 portions of salt in parts by weight.
Mouse intestinal obstruction agent specific the preparation method comprises the following steps:
Step 1 weighs 20 portions of maize flours, 22 parts of dregs of beans, 5 portions of sesame oil and 5 portions of peanuts in parts by weight, adds 10 parts of water mixing
Conventional mouse food is made in drying after uniformly, spare；
Step 2 weighs 10 parts of beef flavors and 3 portions of salt after mixing in parts by weight, and addition is equivalent to its total volume 2
Water again dissolves it sufficiently, obtains auxiliary material, spare；
Step 3 weighs 10 parts of high-hydroscopicity bamboo fibre polysaccharide, conventional mouse food 5 parts, auxiliary material 2 in parts by weight
Part, it is spare；
The high-hydroscopicity bamboo fibre polysaccharide weighed in step 3 is mixed well with the conventional mouse food and is made by step 4
Mixture A, for use；
The auxiliary material weighed in step 3 is added in hand pressure sprayer step 5, with hand pressure sprayer by solution
It is uniformly sprayed on mixture A, during which ceaselessly stirs, obtain mixture B, mixture B is pressed into striped composite at this time；
Step 6 will obtain mixture B and place for 24 hours under the conditions of 80 DEG C in step 5, permeate auxiliary material solution slowly
Into striped composite to get the intestinal obstruction agent, normal temperature storage after packed packaging.
Comparative example 1
The preparation of conventional mouse food: according to parts by weight by 15 parts of maize flour, 13 parts of dregs of beans, 3 parts of sesame oil, 3 parts of peanut mixing
Uniformly, water is uniformly sprayed on the mixture with hand pressure sprayer, during which ceaselessly stirs mixture, object to be mixed presents stronger
Stop sprinkling when sticky, mixture is pressed into striped composite at this time, is steadily placed in 60 DEG C of baking ovens for 24 hours, to be made normal
Mouse food is advised, packed normal temperature storage is spare.
Comparative example 2
The preparation of conventional mouse food: according to parts by weight by 20 parts of maize flour, 22 parts of dregs of beans, 5 parts of sesame oil, 8 parts of peanut mixing
Uniformly, water is uniformly sprayed on the mixture with hand pressure sprayer, during which ceaselessly stirs mixture, object to be mixed presents stronger
Stop sprinkling when sticky, mixture is pressed into striped composite at this time, is steadily placed in 48h in 60 DEG C of baking ovens, to be made normal
Mouse food is advised, packed normal temperature storage is spare.
Comparative example 3
A kind of mouse intestinal obstruction agent, is made of following raw material according to parts by weight: 10 parts of high-hydroscopicity bamboo fibre polysaccharide.
The effect of mouse intestinal obstruction agent provided by the present application is illustrated below.
1, mouse gnaws situation observation
1.1 test method
Areal, identical ambient growth, small white mouse (3.52 ± 0.48 monthly age) 50 similar in weight are chosen, male and female are each
10, five groups are randomly divided into, respectively as three experimental groups and two control groups, every group of male and female each 5.1,2,3 group of embodiment
As experimental group, comparative example 1, comparative example 2,3 groups of comparative example respectively as a control group, by the mouse intestinal obstruction agent of experimental group with it is right
The different location of receptacle is eaten while be put into according to group conventional mouse provided, observes and records the case where small white mouse gnaws mouse food.
1.2 result
The result shows that the mouse that gnaws of embodiment 1-3 group and comparative example 1-3 group is respectively 10,12,9,10,8
Only, 1, by the comparing result of each embodiment and comparative example 1,2 it is found that mouse intestinal obstruction agent provided by the invention can increase mouse
The probability gnawed；By the comparing result of each embodiment and comparative example 3 it is found that by high-hydroscopicity bamboo fibre polysaccharide and conventional mouse food,
Essence mixing, can increase mouse gnaws rate.
2, mouse intestinal obstruction agent addition bark, grass roots are to each position observation experiment after the lethal effect and death of small white mouse
2.1 test method
Areal, identical ambient growth, small white mouse (3.52 ± 0.48 monthly age) 40 similar in weight are chosen, male and female are each
20, it is divided into a control group CK, three experimental groups, every group 10, half male and half female is separately fed.The daily feeding pair of control group
Routine mouse prepared by ratio 1 eats 30g, and 1,2 and 3 every small white mouse of experimental group distinguishes feeding embodiment 3 (due to each embodiment daily
The effect of the mouse intestinal obstruction agent of preparation is essentially identical, therefore the mouse intestinal obstruction agent being prepared only with embodiment 3 is imitated
Fruit evaluation) provide mouse intestinal obstruction agent 10g, embodiment 3 mouse intestinal obstruction agent 10g and grass roots 10g, 3 mouse intestines of embodiment
Block agent 10g and bark 10g.The weight of the every small white mouse in record experiment front and back.By small white mouse of the experimental group after dead and right
According to group, dead small white mouse is not dissected, and is carried out microscope to each internal organs of small white mouse after dissection and is taken pictures, observes and analyze.
Off-test statistical experiment group death small white mouse weight and control group small white mouse weight, time-to-live arrange dissection
Photo.
2.2 result
It the results are shown in Table 1.
The weight comparison of the experiment of table 1 front and back
2 time-to-live of table
By table 1 and table 2 it is found that experimental group small white mouse food ration is reduced by day, the state of mind is deteriorated, under final weight average
5.5-8.5 grams of drop, death time are 2-4 days, and the edible influence to mouse intestinal obstruction agent of grass roots, bark is without significant difference.
Fig. 1-5 is small white mouse internal anatomy.The results show that experimental group finds that stomach of rats inner surface has a black lesion, in stomach
Content is less and black lesion occurs；Large intestine has different degrees of blocking；After being inflated at the small white mouse anus of part
High-hydroscopicity bamboo fibre polysaccharide is completely plugged, has no this phenomenon after the dissection of control group mouse, and the control group state of mind is good, takes the photograph
Food rule is normal, has no adverse reaction, stomach, large intestine and anus are normal.
3, the agent of mouse intestinal obstruction is to each position observation experiment after the lethal effect and death of LE mouse
3.1 test method
Areal, identical ambient growth, LE mouse (3.52 ± 0.48 monthly age) 40 similar in weight are chosen, is divided into 4
Group is divided into a control group CK, and three experimental groups, every group 10, half male and half female is separately fed.It is old that every LE is recorded before experiment
The weight of mouse.Routine mouse prepared by the daily feeding comparative example 2 of control group eats 30g, and every LE mouse of experimental group distinguishes feeding daily
Mouse intestinal obstruction agent 30g prepared by 1,2,3 group of embodiment, and each group food ration is counted in daily 5:00pm.Experimental group is dead
LE mouse and control group LE mouse after dying are dissected, and carry out microscope to each internal organs after dissection and take pictures, observe and analyze.
Off-test statistical experiment group death LE mouse weight and control group LE mouse weight, time-to-live arrange dissection
Photo.
3.2 result
It the results are shown in Table 3.
The weight comparison of the experiment of table 3 front and back
4 time-to-live of table
By table 3 and table 4 it is found that each experimental group LE mouse food ration is reduced by day, the state of mind is deteriorated, final weight average
62-100 grams of decline, and as the content of high-hydroscopicity bamboo fibre gradually increases, LE mouse death is faster, and the death time is in 4-42
It.Control group LE mouse Feeding regularity is normal, and the state of mind is good, has no adverse reaction, and after experimental group is dead, control group is not dead
It dies.
Fig. 6-11 is LE mouse internal anatomy, the results show that each experimental group finds that experimental group mouse stomach surface has black disease
Become, content is less in stomach and black lesion occurs；Large intestine has different degrees of blocking；It is swollen at the LE mouse anus of part
High-hydroscopicity bamboo fibre polysaccharide after swollen is completely plugged, has no this phenomenon after the dissection of control group mouse.
Result above proves that mouse intestinal obstruction agent provided by the invention can increase the probability of gnawing of mouse, blocks mouse
Stomach, and cause it gradually dead, and as the content of high-hydroscopicity bamboo fibre in intestinal obstruction agent gradually increases, mouse is dead
It is faster.
It should be noted that involved in claims of the present invention when numberical range, it is thus understood that each numberical range
Any one numerical value can be selected between two endpoints and two endpoints, due to step method and the Examples 1 to 3 phase of use
Together, it repeats in order to prevent, the present invention describes preferred embodiment, and once a person skilled in the art knows basic wounds
The property made concept, then additional changes and modifications may be made to these embodiments.So the following claims are intended to be interpreted as includes
Preferred embodiment and all change and modification for falling into the scope of the invention.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, made any modification, replacement, improvement etc. are all included in the scope of protection of the present invention.
Claims (8)
1. application of the high-hydroscopicity bamboo fibre polysaccharide in preparation mouse intestinal obstruction agent, the high-hydroscopicity bamboo fibre polysaccharide have
The molecular structure as shown in formula (I):
Wherein: n is 2000~8000.
2. a kind of mouse intestinal obstruction agent, which is characterized in that the intestinal obstruction agent includes high-hydroscopicity bamboo described in claim 1
Fibrous polysaccharaide.
3. mouse intestinal obstruction agent according to claim 2, which is characterized in that the intestinal obstruction agent according to parts by weight by with
Lower component is made: 5-10 parts of high-hydroscopicity bamboo fibre polysaccharide, conventional mouse eat 5-10 parts, 2-3 parts of auxiliary material.
4. mouse intestinal obstruction agent according to claim 3, which is characterized in that the intestinal obstruction agent is according to parts by weight by such as
Lower raw material is prepared: 8 parts of high-hydroscopicity bamboo fibre polysaccharide, conventional mouse eat 6 parts, 2 parts of auxiliary material.
5. mouse intestinal obstruction agent according to claim 3 or 4, which is characterized in that the routine mouse food is according to parts by weight
Add 10 parts of water to dry after mixing by 15-20 portions of maize flours, 13-22 parts of dregs of beans, 3-5 portions of sesame oil and 3-5 portions of peanuts to be made.
6. mouse intestinal obstruction agent according to claim 3 or 4, which is characterized in that the auxiliary material is by following parts by weight
Material composition: 4-10 parts of essence, 2-3 parts of salt.
7. mouse intestinal obstruction agent according to claim 6, which is characterized in that the essence is beef flavor, carrot perfume
One of essence or peanut essence.
8. the preparation method of mouse intestinal obstruction agent according to claim 3, which is characterized in that specifically includes the following steps:
Step 1 weighs 15-20 portions of maize flours, 13-22 parts of dregs of beans, 3-5 portions of sesame oil and 3-5 portions of peanuts, 10 parts in parts by weight
Water, conventional mouse food is made in drying after mixing, spare；
Step 2 weighs 4-10 parts of essence, 2-3 parts of salt after mixing in parts by weight, and addition is equivalent to 2 times of its total volume
Water dissolves it sufficiently, obtains auxiliary material, spare；
Step 3 weighs 5-10 parts of high-hydroscopicity bamboo fibre polysaccharide, the conventional mouse food 5-10 of gained in step 1 in parts by weight
2-3 parts of auxiliary material of gained, spare in part and step 2；
The high-hydroscopicity bamboo fibre polysaccharide weighed in step 3 is mixed well to be made and is mixed by step 4 with the conventional mouse food
Object A, for use；
The auxiliary material weighed in step 3 is uniformly sprayed on the mixture A by step 5, obtains mixture B；
Step 6 will obtain mixture B and place 24-48h under the conditions of 40-80 DEG C to get the intestinal obstruction agent in step 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910871329.8A CN110476984B (en) | 2019-09-16 | 2019-09-16 | Application of high-water-absorptivity bamboo fiber polysaccharide in preparation of rat intestinal stalk resistance agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910871329.8A CN110476984B (en) | 2019-09-16 | 2019-09-16 | Application of high-water-absorptivity bamboo fiber polysaccharide in preparation of rat intestinal stalk resistance agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110476984A true CN110476984A (en) | 2019-11-22 |
| CN110476984B CN110476984B (en) | 2021-07-13 |
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| CN115005235A (en) * | 2022-06-28 | 2022-09-06 | 辽宁微科生物工程有限公司 | Microbial rodenticide |
| CN115606594A (en) * | 2022-09-28 | 2023-01-17 | 西南民族大学 | Rat-killing bait and preparation method thereof |
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| CN115606594A (en) * | 2022-09-28 | 2023-01-17 | 西南民族大学 | Rat-killing bait and preparation method thereof |
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| CN110476984B (en) | 2021-07-13 |
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