CN110476984B - Application of high-water-absorptivity bamboo fiber polysaccharide in preparation of rat intestinal stalk resistance agent - Google Patents

Application of high-water-absorptivity bamboo fiber polysaccharide in preparation of rat intestinal stalk resistance agent Download PDF

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CN110476984B
CN110476984B CN201910871329.8A CN201910871329A CN110476984B CN 110476984 B CN110476984 B CN 110476984B CN 201910871329 A CN201910871329 A CN 201910871329A CN 110476984 B CN110476984 B CN 110476984B
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黎川
补约阿的子
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Sichuan Xinxinjiaoyang Biological Technology Co ltd
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    • AHUMAN NECESSITIES
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Abstract

The invention belongs to the technical field of rat damage defense, and particularly discloses application of high-water-absorptivity bamboo fiber polysaccharide in preparation of a rat intestinal stalk resistance agent. The invention also provides a rat intestinal stalk resistance agent which is prepared from the following raw materials in parts by weight: 5-10 parts of high-water-absorption bamboo fiber polysaccharide, 5-10 parts of conventional rat food and 2-3 parts of essence. The rat intestinal stalk resistance agent provided by the invention has the advantages of simple and effective preparation process, easily available raw materials, capability of blocking and killing the intestinal tract of rats after eating, quick response, good palatability and no pollution to the environment, and the high water absorption bamboo fiber polysaccharide has good water absorption and water retention capacity, can quickly absorb water to promote the expansion of the gastrointestinal tract of the rats or the solid state of food in the gastrointestinal tract after the rats eat the agent, forms blockage in the intestinal tract, and kills the rats due to the compensation of the digestive absorption function of the gastrointestinal tract, so that the agent is a novel environment-friendly rat poison for preventing and treating rat damage.

Description

Application of high-water-absorptivity bamboo fiber polysaccharide in preparation of rat intestinal stalk resistance agent
Technical Field
The invention relates to the technical field of rat damage defense, and particularly relates to application of high-water-absorptivity bamboo fiber polysaccharide in preparation of a rat intestinal stalk resistance agent.
Background
The mice in the world are various in species, large in quantity, wide in distribution and high in propagation speed, not only are harmful to buildings, crops, forest trees and the like, but also carry and infect various viruses, and the mice are natural hosts of various epidemic diseases such as plague, rabies, encephalitis, brucellosis pseudotuberculosis and jaundice. The rat damage affects the life of people, destroys the ecological environment and causes immeasurable loss to national economy. In recent years, the drug resistance of rats is enhanced due to the abuse of chemical drugs, and rat damage is in a serious aggravation trend, so that the rat damage becomes one of the problems of biological disasters in the world.
The chemical rodenticide is the most main rodenticide at present, has quick response, immediately dies mice after using the medicaments, has the main function of effectively killing the mice or destroying the reproductive capacity of the mice, but pollutes the environment, is easy to generate secondary toxicity to people and livestock, can make the mice generate resistance, has no specificity, generates harm to natural enemies of the mice, and further makes the mice aggravated; and because the mouse is suspectable in nature and high in intelligence quotient for survival, other mice are very easy to be suspectable due to the characteristics of acute deratization of the chemical rodenticide, and the deratization efficiency is reduced. Therefore, the development of a novel slow rodenticide which is nontoxic, harmless, free of environmental pollution, free of drug resistance and safe to use has become a problem to be solved.
For the traditional rodenticide, whether the drug residue outside the rat body or in the rat body exists, more or less of the drug residue can cause certain harm to plants or other secondary animals, and the high-hydroscopicity bamboo fiber polysaccharide is non-toxic and harmless, has strong water absorption and retention capacity, can improve soil and prevent water and soil loss, is used for preventing rat damage, and can generate good social benefit and ecological benefit. How to prepare the high water absorption bamboo fiber polysaccharide into a proper medicament to improve the palatability of the medicament becomes an urgent problem to be solved.
Disclosure of Invention
In order to solve the problems that the existing rodenticide is easy to cause mice to generate resistance, the deratization efficiency is low and the like, the invention provides the following technical scheme:
the invention aims to provide an application of high-water-absorptivity bamboo fiber polysaccharide in preparation of a rat intestinal stalk inhibitor, wherein the high-water-absorptivity bamboo fiber polysaccharide has a molecular structure shown as a formula (I):
Figure BDA0002202904250000021
wherein: n is 2000 to 8000.
The preparation method of the high water absorption bamboo fiber polysaccharide comprises the following steps:
s1, weighing 100g of bamboo fiber fine particles, 20g of acrylic acid, 300g of deionized water, 1g of potassium persulfate and 1g of phenyl bis (2,4, 6-trimethylbenzoyl) phosphine oxide according to parts by weight for later use;
wherein the bamboo fiber fine particles are prepared by the following method: crushing annual phyllostachys pubescens and sieving with a 100-mesh sieve to obtain bamboo fiber fine particles;
s2, mixing and grinding the bamboo fiber fine particles weighed in the S1 and potassium persulfate, and then reacting for 4 hours to obtain the alkalized bamboo fiber;
s3, adding deionized water weighed in S1 into the alkalized bamboo fiber obtained in S2, then adding acrylic acid weighed in S1, stirring and reacting for 20min at 50 ℃, then preserving heat for 1h, and washing to obtain reaction bamboo fiber;
s4, adding phenylbis (2,4, 6-trimethylbenzoyl) phosphine oxide to the reacted bamboo fiber obtained in S3, followed by sealing, and then heating at 45 deg.C60Irradiating for 2h by Co-gamma rays under the irradiation dose of 5kGy, washing and drying to obtain the macromolecular bamboo fiber polysaccharide.
The second purpose of the invention is to provide the rat intestinal stalk resistance agent, wherein the main effective component of the intestinal stalk resistance agent is high water absorption bamboo fiber polysaccharide.
Preferably, the intestinal obstruction agent is prepared from the following components in parts by weight: 5-10 parts of high-water-absorption bamboo fiber polysaccharide, 5-10 parts of conventional rat food and 2-3 parts of auxiliary materials.
Further preferably, the intestinal obstruction agent is prepared from the following raw materials in parts by weight: 8 parts of high-water-absorption bamboo fiber polysaccharide, 6 parts of conventional rat food and 2 parts of auxiliary materials.
Preferably, the conventional mouse food is prepared by uniformly mixing 15-20 parts of corn flour, 13-22 parts of soybean meal, 3-5 parts of sesame oil and 3-5 parts of peanut with 10 parts of water and then drying.
Preferably, the auxiliary material consists of the following substances in parts by weight: 4-10 parts of essence and 2-3 parts of salt.
Further preferably, the essence is one of beef essence, carrot essence or peanut essence.
The invention also provides a preparation method of the rat intestinal stalk inhibitor, which comprises the following steps:
step 1, weighing 15-20 parts of corn flour, 13-22 parts of bean pulp, 3-5 parts of sesame oil, 3-5 parts of peanuts and 10 parts of water according to parts by weight, uniformly mixing, and drying to obtain a conventional rat food for later use;
step 2, weighing 4-10 parts of essence and 2-3 parts of salt according to parts by weight, uniformly mixing, and adding water with the volume 2 times of the total volume of the mixture to fully dissolve the mixture to obtain an auxiliary material for later use;
step 3, weighing 5-10 parts of high-water-absorptivity bamboo fiber polysaccharide, 5-10 parts of the conventional rat food obtained in the step 1 and 2-3 parts of the auxiliary material obtained in the step 2 according to parts by weight for later use;
step 4, fully and uniformly mixing the high-water-absorptivity bamboo fiber polysaccharide weighed in the step 3 with the conventional rat food to prepare a mixture A for later use;
step 5, uniformly spraying the auxiliary material weighed in the step 3 on the mixture A to obtain a mixture B;
and 6, standing the mixture B obtained in the step 5 at the temperature of 40-80 ℃ for 24-48h to obtain the intestinal stalk blocker.
Compared with the prior art, the invention has the beneficial effects that:
1. the rat intestinal stalk resistance agent provided by the invention has the advantages of simple and convenient preparation process, easily controlled quality standard and good palatability of the prepared finished product; the used materials can be degraded, the gastrointestinal functions are changed by physical action to prevent and control mouse damage, and the pesticide is nontoxic to mice and environment and pollution-free; the cost is low, and the popularization and the application are easy;
2. the high-water-absorption bamboo fiber polysaccharide is a high-molecular material with a particularly strong water absorption function, is non-toxic and harmless, and absorbs water because the surfaces of bamboo fiber molecules have a large number of hydrophilic groups, hydroxyl groups, carboxyl groups and a large number of pores, so that water in the stomach and intestinal tracts of mice can be absorbed, the mice after eating generate craving feeling, and then continue to drink water, and finally the mice die due to the enlargement of water absorption of intestines and stomach and the blockage of the intestinal tracts, are harmless to other secondary animals, do not pollute the environment, are safe to use, and do not generate drug resistance in the mice.
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To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, and it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope.
FIG. 1 is a graph showing a comparison of the anatomy of a white mouse eating a rat chow and a rat intestinal infarction inhibitor after death;
FIG. 2 is a graph showing the gastric contrast of a white mouse eating the common mouse food and the mouse intestinal stalk inhibitor after death;
FIG. 3 is a graph showing the anatomical comparison of the stomach of a white mouse eating the common mouse food and the mouse intestinal stalk inhibitor after death;
FIG. 4 is a graph showing the anatomical comparison of the large intestine of a white mouse after death, which has eaten the common mouse food and the mouse intestinal stalk inhibitor;
FIG. 5 is a comparison of anus anatomy after death of mice fed with rat chow and rat intestinal infarction inhibitor;
FIG. 6 is a graph showing the anatomical comparison of killed LE mice fed with rat chow and rat intestinal infarction inhibitor;
FIG. 7 is a graph comparing the stomach of a LE mouse after death, eating common mouse chow and mouse intestinal infarction inhibitor;
FIG. 8 is a graph showing a comparison of stomach anatomy after death of LE mice fed with rat chow and rat intestinal infarction inhibitor;
FIG. 9 is a graph showing a comparison of the large intestine anatomy of LE mice fed with rat chow and rat intestinal infarction reducer after death;
FIG. 10 is a graph showing comparison of anus after death of LE mice fed with rat chow and rat intestinal infarction inhibitor;
FIG. 11 is a graph comparing anus anatomy after death of LE mice fed with rat chow and rat intestinal infarction agent;
in fig. 1-11, the left images are all control groups, and the right images are all experimental groups.
Detailed Description
The present invention will be further clearly understood from the following examples and experimental examples, which are not intended to limit the scope of the present invention. Many insubstantial modifications and adaptations of those skilled in the art in light of the above teachings will fall within the scope of the invention.
The invention provides an application of high water absorption bamboo fiber polysaccharide in preparing a rat intestinal stalk resistance agent, which is used for preventing and treating rat damage and has the function of reducing the rat damage by controlling the number of rat damage populations;
the high water absorption bamboo fiber polysaccharide has a molecular structure shown in a formula (I):
Figure BDA0002202904250000061
wherein: n is 2000 to 8000;
the high water absorption bamboo fiber polysaccharide is a high molecular material with a very strong water absorption function, is non-toxic and harmless, can absorb water in the stomach and intestinal tract of a mouse after the mouse eats the high water absorption bamboo fiber polysaccharide, enables the mouse after eating to feel eager, further continues drinking water, finally dies due to the water absorption expansion of the stomach and the intestinal tract blockage, is harmless to other secondary animals, does not pollute the environment, is safe to use, and does not generate drug resistance in the mouse.
The high water absorption bamboo fiber polysaccharide is tasteless, and can be used together with other non-effective components (such as conventional mouse food and essence) to prepare the mouse intestinal obstruction agent, so that the ingestion rate of mice can be increased, and the deratization rate is increased. The specific implementation method comprises the following steps:
example 1
The rat intestinal stalk resistance agent comprises the following raw materials in parts by weight: 5 parts of high-water-absorption bamboo fiber polysaccharide, 5 parts of conventional rat food and 2 parts of auxiliary materials;
the high water absorption bamboo fiber polysaccharide has a structure shown in a formula (I):
Figure BDA0002202904250000071
wherein the conventional rat food is prepared from 15 parts of corn flour, 13 parts of bean pulp, 3 parts of sesame oil and 3 parts of peanuts by weight;
the auxiliary material is prepared from 4 parts of beef essence and 2 parts of salt according to parts by weight.
The concrete preparation method of the rat intestinal stalk resistance agent comprises the following steps:
step 1, weighing 15 parts of corn flour, 13 parts of bean pulp, 3 parts of sesame oil and 3 parts of peanuts according to parts by weight, adding 10 parts of water, uniformly mixing, and drying to prepare a conventional rat food for later use;
step 2, weighing 4 parts of beef essence and 2 parts of salt according to parts by weight, uniformly mixing, and adding water with the volume 2 times of the total volume of the beef essence to fully dissolve the beef essence and the salt to obtain an auxiliary material for later use;
step 3, weighing 5 parts of high-water-absorptivity bamboo fiber polysaccharide, 10 parts of conventional rat food and 3 parts of the auxiliary material obtained in the step 2 according to the parts by weight for later use;
step 4, fully and uniformly mixing the high-water-absorptivity bamboo fiber polysaccharide weighed in the step 3 with the conventional rat food to prepare a mixture A for later use;
step 5, adding the auxiliary materials weighed in the step 3 into a hand-pressed sprayer, uniformly spraying the solution on the mixture A by using the hand-pressed sprayer, continuously turning over the mixture A to obtain a mixture B, and pressing the mixture B into a strip-shaped compound;
and 6, standing the mixture B obtained in the step 5 at 40 ℃ for 48 hours to enable the auxiliary material solution to slowly permeate into the strip-shaped compound, so as to obtain the intestinal stalk blocker, and storing at normal temperature after bagging and packaging.
Example 2
The rat intestinal stalk resistance agent comprises the following raw materials in parts by weight: 8 parts of high-water-absorption bamboo fiber polysaccharide, 6 parts of conventional rat food and 2 parts of auxiliary materials;
the high water absorption bamboo fiber polysaccharide has a structure shown in a formula (I):
Figure BDA0002202904250000081
wherein, the conventional rat food is prepared from 18 parts of corn flour, 18 parts of bean pulp, 4 parts of sesame oil and 4 parts of peanuts by weight;
the auxiliary material is prepared from 7 parts of beef essence and 3 parts of salt according to parts by weight.
The concrete preparation method of the rat intestinal stalk resistance agent comprises the following steps:
step 1, weighing 18 parts of corn flour, 18 parts of bean pulp, 4 parts of sesame oil and 4 parts of peanuts according to parts by weight, adding 10 parts of water, uniformly mixing, and drying to prepare a conventional rat food for later use;
step 2, weighing 7 parts of beef essence and 3 parts of salt according to parts by weight, uniformly mixing, and adding water with the volume 2 times of the total volume of the beef essence to fully dissolve the beef essence and the salt to obtain an auxiliary material for later use;
step 3, weighing 8 parts of high-water-absorptivity bamboo fiber polysaccharide, 6 parts of conventional rat food and 2 parts of the auxiliary material obtained in the step 2 according to the parts by weight for later use;
step 4, fully and uniformly mixing the high-water-absorptivity bamboo fiber polysaccharide weighed in the step 3 with the conventional rat food to prepare a mixture A for later use;
step 5, adding the auxiliary materials weighed in the step 3 into a hand-pressed sprayer, uniformly spraying the solution on the mixture A by using the hand-pressed sprayer, continuously turning over the mixture A to obtain a mixture B, and pressing the mixture B into a strip-shaped compound;
and 6, standing the mixture B obtained in the step 5 at 60 ℃ for 36 hours to enable the auxiliary material solution to slowly permeate into the strip-shaped compound, so as to obtain the intestinal stalk blocker, and storing at normal temperature after bagging and packaging.
Example 3
The rat intestinal stalk resistance agent comprises the following raw materials in parts by weight: 10 parts of high-water-absorption bamboo fiber polysaccharide, 10 parts of conventional rat food and 3 parts of auxiliary materials;
the high water absorption bamboo fiber polysaccharide has a structure shown in a formula (I):
Figure BDA0002202904250000091
wherein the conventional rat food is prepared from 20 parts of corn flour, 22 parts of bean pulp, 5 parts of sesame oil and 5 parts of peanuts by weight;
the auxiliary material is prepared from 10 parts of beef essence and 3 parts of salt according to parts by weight.
The concrete preparation method of the rat intestinal stalk resistance agent comprises the following steps:
step 1, weighing 20 parts of corn flour, 22 parts of bean pulp, 5 parts of sesame oil and 5 parts of peanuts according to parts by weight, adding 10 parts of water, uniformly mixing, and drying to prepare a conventional rat food for later use;
step 2, weighing 10 parts of beef essence and 3 parts of salt according to parts by weight, uniformly mixing, and adding water with the volume 2 times of the total volume of the beef essence to fully dissolve the beef essence and the salt to obtain an auxiliary material for later use;
step 3, weighing 10 parts of high-water-absorptivity bamboo fiber polysaccharide, 5 parts of conventional rat food and 2 parts of auxiliary materials in parts by weight for later use;
step 4, fully and uniformly mixing the high-water-absorptivity bamboo fiber polysaccharide weighed in the step 3 with the conventional rat food to prepare a mixture A for later use;
step 5, adding the auxiliary materials weighed in the step 3 into a hand-pressed sprayer, uniformly spraying the solution on the mixture A by using the hand-pressed sprayer, continuously turning over the mixture A to obtain a mixture B, and pressing the mixture B into a strip-shaped compound;
and 6, standing the mixture B obtained in the step 5 at 80 ℃ for 24 hours to enable the auxiliary material solution to slowly permeate into the strip-shaped compound, so as to obtain the intestinal stalk blocker, and storing at normal temperature after bagging and packaging.
Comparative example 1
Preparation of conventional rat chow: uniformly mixing 15 parts of corn flour, 13 parts of bean pulp, 3 parts of sesame oil and 3 parts of peanuts according to the parts by weight, uniformly spraying water on the mixture by using a hand-pressed sprayer, continuously turning over the mixture during the period, stopping spraying when the mixture shows strong viscosity, pressing the mixture into a strip-shaped compound, stably placing the compound in a 60 ℃ oven for 24 hours to prepare the conventional rat food, and bagging the conventional rat food for storage at normal temperature for later use.
Comparative example 2
Preparation of conventional rat chow: uniformly mixing 20 parts of corn flour, 22 parts of bean pulp, 5 parts of sesame oil and 8 parts of peanuts according to the parts by weight, uniformly spraying water on the mixture by using a hand-pressed sprayer, continuously turning over the mixture during the period, stopping spraying when the mixture shows strong viscosity, pressing the mixture into a strip-shaped compound, stably placing the compound in a 60 ℃ oven for 48 hours to prepare the conventional rat food, and bagging the conventional rat food for storage at normal temperature for later use.
Comparative example 3
The rat intestinal stalk resistance agent comprises the following raw materials in parts by weight: 10 parts of high water absorption bamboo fiber polysaccharide.
The effect of the rat intestinal stalk blocker provided by the present application is explained below.
1. Observation of mouse gnawing condition
1.1 test methods
50 mice (3.52 +/-0.48 months old) which grow in the same area and under the same environment and have similar weight are selected, 10 males and females are randomly divided into five groups, and the five groups are respectively used as three experimental groups and two control groups, and each group comprises 5 females and males. The experimental groups of examples 1, 2 and 3 are used as experimental groups, the comparative groups of comparative examples 1, 2 and 3 are used as control groups respectively, the rat intestinal obstruction agent of the experimental groups and the conventional rat food provided by the control groups are placed at different positions of a feeding room at the same time, and the gnawing condition of the white rats is observed and recorded.
1.2 results
The results show that 10 mice, 12 mice, 9 mice, 10 mice, 8 mice and 1 mouse in the groups of examples 1-3 and comparative examples 1-3 respectively, and the comparison results of the examples and comparative examples 1 and 2 show that the rat intestinal stalk inhibitor provided by the invention can increase the probability of rat gnawing; from the comparison results of the examples and the comparative example 3, it is known that the biting rate of rats can be increased by mixing the high water absorption bamboo fiber polysaccharide with the conventional rat food and essence.
2. Experiment for killing mouse by adding mouse intestinal obstruction agent into bark and grass root and observing each part after death
2.1 test methods
40 mice (3.52 +/-0.48 months old) which grow in the same area and in the same environment and have similar weight are selected, 20 mice are divided into a control group CK and three experimental groups, wherein each experimental group comprises 10 mice and half mice are fed separately. The control group was fed 30g of the conventional rat prepared in comparative example 1 per day, and the experimental groups 1, 2 and 3 were fed 10g of the rat intestinal obstruction agent provided in example 3 (the effect evaluation was performed only using the rat intestinal obstruction agent prepared in example 3 because the effect of the rat intestinal obstruction agent prepared in each example was substantially the same) per day, 10g of the rat intestinal obstruction agent provided in example 3, and 10g of grass roots, 10g of the rat intestinal obstruction agent provided in example 3, and 10g of bark. The body weight of each mouse was recorded before and after the experiment. And dissecting the dead mice in the experimental group and the mice in the control group which are not dead, and taking pictures of organs of the mice under a microscope after dissection, observing and analyzing.
And after the test is finished, counting the weight of dead mice in the experimental group and the weight of mice in the control group, survival time and finishing anatomical pictures.
2.2 results
The results are shown in Table 1.
TABLE 1 weight comparison before and after experiment
Figure BDA0002202904250000121
TABLE 2 survival time
Figure BDA0002202904250000122
Figure BDA0002202904250000131
As can be seen from tables 1 and 2, the food intake of the white mice in the experimental group is reduced day by day, the mental state is deteriorated, the final weight is reduced by 5.5-8.5 g averagely, the death time is 2-4 days, and the influence of the eating of the grass roots and the barks on the intestinal stalk resistance agent of the mice is not obviously different.
FIGS. 1-5 are anatomical views of mice. The results show that the experimental group finds that the inner surface of the stomach of the mouse has black lesions, the content in the stomach is less and the mice have black lesions; the large intestine is blocked to different degrees; the anus of part of mice is completely blocked by the expanded high-water-absorptivity bamboo fiber polysaccharide, the phenomenon does not occur after the mice in the control group are dissected, the mental state of the control group is good, the feeding rule is normal, no adverse reaction occurs, and the stomach, the large intestine and the anus are normal.
3. Lethal effect of mouse intestinal stalk inhibitor on LE mouse and observation experiment of each part after death
3.1 test methods
40 LE mice (3.52 +/-0.48 months old) growing in the same area and in the same environment and with similar weight are selected and divided into 4 groups, namely a control group CK and three experimental groups, wherein each group comprises 10 mice and each half of the mice are fed separately. Body weight of each LE mouse was recorded prior to the experiment. The control group was fed 30g of the conventional rats prepared in comparative example 2 per day, and each LE rat in the experimental group was fed 30g of the mouse ileus agent prepared in examples 1, 2 and 3 per day, respectively, and the food intake of each group was counted at 5:00pm per day. And dissecting the killed LE mice in the experimental group and the killed LE mice in the control group, taking pictures of all organs by a microscope after dissection, and observing and analyzing the pictures.
And (4) counting the weights of dead LE mice in the experimental group and dead LE mice in the control group after the experiment is finished, survival time and sorting anatomical pictures.
3.2 results
The results are shown in Table 3.
TABLE 3 weight comparison before and after experiment
Figure BDA0002202904250000141
TABLE 4 survival time
Figure BDA0002202904250000142
Figure BDA0002202904250000151
As can be seen from tables 3 and 4, food intake of LE mice of each experimental group decreased day by day, mental state became worse, and final weight decreased by 62-100 g on average, and along with the increasing content of the super absorbent bamboo fiber, the LE mice died faster, and the death time was 4-42 days. The LE mice in the control group have normal feeding rule, good mental state and no adverse reaction, and the control group does not die after the experimental group dies.
6-11 are LE mouse anatomy diagrams showing that the mice in each experimental group have black lesions on the stomach surface, less contents in the stomach and black lesions; the large intestine is blocked to different degrees; the anus of part of LE mice is completely blocked by the expanded high-water-absorptivity bamboo fiber polysaccharide, and the phenomenon is not shown after the control mice are dissected.
The results prove that the mouse intestinal obstruction agent provided by the invention can increase the feeding probability of mice, block the intestines and stomach of the mice and cause the mice to die gradually, and the mice die faster along with the gradual increase of the content of the high-hydroscopicity bamboo fibers in the intestinal obstruction agent.
It should be noted that when the following claims refer to numerical ranges, it should be understood that both endpoints of each numerical range and any numerical value between the two endpoints can be selected, and since the steps and methods adopted are the same as those in embodiments 1 to 3, the present invention describes preferred embodiments in order to prevent redundant description, but once a person skilled in the art knows the basic inventive concept, other changes and modifications can be made to these embodiments. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, substitutions, improvements, etc. made within the spirit and principle of the present invention are included in the scope of the present invention.

Claims (7)

1. The application of the high water absorption bamboo fiber polysaccharide in preparing the rat intestinal stalk resistance agent is characterized in that the high water absorption bamboo fiber polysaccharide has a molecular structure as shown in a formula (I):
Figure FDA0003001717680000011
wherein: n is 2000 to 8000.
2. The rat intestinal stalk inhibitor is characterized by being prepared from the following components in parts by weight: the high water absorption bamboo fiber polysaccharide of claim 1, which comprises 5-10 parts of conventional rat food and 2-3 parts of auxiliary materials.
3. The agent for blocking intestinal stalk of mice according to claim 2, wherein the agent for blocking intestinal stalk is prepared from the following raw materials in parts by weight: 8 parts of high-water-absorption bamboo fiber polysaccharide, 6 parts of conventional rat food and 2 parts of auxiliary materials.
4. The agent for blocking intestinal stalk of mouse as claimed in claim 2 or 3, wherein the conventional mouse food is prepared by mixing 15-20 parts of corn flour, 13-22 parts of soybean meal, 3-5 parts of sesame oil and 3-5 parts of peanut with 10 parts of water, and drying.
5. The rat intestinal stalk blocker according to claim 4, wherein the auxiliary material is composed of the following substances in parts by weight: 4-10 parts of essence and 2-3 parts of salt.
6. The mouse intestinal stalk blocker according to claim 5, wherein the essence is one of beef essence, carrot essence or peanut essence.
7. The preparation method of the mouse intestinal stalk blocker according to claim 5, comprising the following steps:
step 1, weighing 15-20 parts of corn flour, 13-22 parts of bean pulp, 3-5 parts of sesame oil, 3-5 parts of peanuts and 10 parts of water according to parts by weight, uniformly mixing, and drying to obtain a conventional rat food for later use;
step 2, weighing 4-10 parts of essence and 2-3 parts of salt according to parts by weight, uniformly mixing, and adding water with the volume 2 times of the total volume of the mixture to fully dissolve the mixture to obtain an auxiliary material for later use;
step 3, weighing 5-10 parts of high-water-absorptivity bamboo fiber polysaccharide, 5-10 parts of the conventional rat food obtained in the step 1 and 2-3 parts of the auxiliary material obtained in the step 2 according to parts by weight for later use;
step 4, fully and uniformly mixing the high-water-absorptivity bamboo fiber polysaccharide weighed in the step 3 with the conventional rat food to prepare a mixture A for later use;
step 5, uniformly spraying the auxiliary material weighed in the step 3 on the mixture A to obtain a mixture B;
and 6, standing the mixture B obtained in the step 5 at the temperature of 40-80 ℃ for 24-48h to obtain the intestinal stalk blocker.
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