CN115005235B - Microbial rodenticide - Google Patents
Microbial rodenticide Download PDFInfo
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- CN115005235B CN115005235B CN202210751580.2A CN202210751580A CN115005235B CN 115005235 B CN115005235 B CN 115005235B CN 202210751580 A CN202210751580 A CN 202210751580A CN 115005235 B CN115005235 B CN 115005235B
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 65
- 239000003128 rodenticide Substances 0.000 title claims abstract description 42
- 239000000463 material Substances 0.000 claims abstract description 54
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 claims abstract description 26
- 241000233866 Fungi Species 0.000 claims abstract description 10
- 239000002054 inoculum Substances 0.000 claims abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 50
- 238000002360 preparation method Methods 0.000 claims description 13
- 208000037487 Endotoxemia Diseases 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 10
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- 229960004192 diphenoxylate Drugs 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 8
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- 229930013292 trichothecene Natural products 0.000 abstract 2
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
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- 241000235349 Ascomycota Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
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- 241001529936 Murinae Species 0.000 description 1
- 206010067482 No adverse event Diseases 0.000 description 1
- 235000019779 Rapeseed Meal Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 241000378866 Trichoderma koningii Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
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- 239000004467 fishmeal Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
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- 238000005259 measurement Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
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- 230000000644 propagated effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
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- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/004—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits rodenticidal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P11/00—Rodenticides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Toxicology (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The application provides a microbial rodenticide, relates to the field of biological control, and comprises a microbial inoculant, wherein the microbial inoculant comprises materials and a trichothecene, the microbial inoculant is a living fungus culture object of the trichothecene, can generate a plurality of aromatic substances, can generate excellent feeding attraction to mice, has excellent palatability, is simple in raw material components and low in cost, and can increase the feeding theft of the mice to the medicaments, so that the rodenticide efficiency is improved. After the living bacteria culture object of the endomyzium curvatum enters the intestinal tract, the intestinal tract is anaerobic and still, and the living bacteria of the endomyzium curvatum, the faeces in the intestinal tract of the mice and the food which is not digested are fermented to generate gas, so that the gas plays a role of a propellant, the generated gas can accelerate the precipitation of barium sulfate and plays a role of blocking, so that the mice can cause acute obstruction in the intestinal tract after eating, and the mice die after being combined with heart rate failure, and the method has the advantages of short death time and high death rate.
Description
Technical Field
The application relates to the field of biological control, in particular to a microbial rodenticide.
Background
Most of the existing phagostimulants added into rodenticides take sugar, protein and fat as raw materials, and spice, aromatic, lipid and other spice substances or chemical components capable of playing a role in phagostimulation, such as glucose, sucrose, corn flour, wheat bran, bean pulp, rapeseed meal, fish meal, various fats, yeast, beef extract and the like are added.
The current murine phagostimulant has the following obvious characteristics: 1. the raw materials have complex constitution and large quality control difficulty, and are not beneficial to industrialized preparation. 2. The preparation cost is high. 3. The preparation process is not based on animal habit, but is more based on human preference, and better food calling effect is not achieved. 4. The bait is not well combined with food, so that mice can be attracted from a distance, and meanwhile, the product has good palatability to achieve the due effect.
Disclosure of Invention
The application aims to provide a microbial rodenticide, which aims to solve the problems of complex raw materials, high quality control difficulty, high preparation cost and poor rodenticide effect of the existing rodenticide.
To achieve the above object, the present application provides a microbial rodenticide comprising, in mass percent: 20 to 80 percent of microbial agent, 10 to 50 percent of barium sulfate, 0.01 to 0.05 percent of diphenoxylate and 0 to 10 percent of grain.
Preferably, the microbial agent comprises a material and the endotoxemia of the knot bag inoculated in the material, wherein the inoculation amount of the endotoxemia of the knot bag is 5-15% of the dry weight of the material.
Preferably, the materials comprise the following components in parts by weight: 40-80 parts of corn flour, 60-80 parts of fresh fungus chaff, 5-15 parts of bean pulp powder and 1-5 parts of ammonium sulfate.
Preferably, the fresh fungus chaff comprises a sterile culture obtained after preparation of edible fungi.
Preferably, the viable count of the endotoxemia of the microbial agent is more than 2 x 10 9 CFU/g。
Preferably, the preparation method of the microbial agent comprises the following steps:
mixing the material and the endotoxemium cinnabarinosum according to the proportion;
controlling the thickness of the material to be 10-15cm, the water content of the material to be 45-48%, the pH of the initial material to be 6.0-6.5, the culture humidity to be 85-100%, the culture temperature to be 30-35 ℃ and the culture period to be 36-48h, thus obtaining the microbial agent.
Preferably, after the microbial agent is obtained, the method further comprises:
and drying the microbial agent, wherein the drying temperature is not higher than 60 ℃, the drying temperature is not higher than 12% of the water content of the material, and the inactivation rate of the endotoxemium cinatum is lower than 5%.
Preferably, the effective viable count in the microbial rodenticide is greater than 4 x 10 8 CFU/g。
Compared with the prior art, the beneficial effects of this application include:
the microbial agent comprises materials and the endotoxemia of the knot bag, the microbial agent is a living bacterial culture object of the endotoxemia of the knot bag, a plurality of aromatic substances can be generated in the living bacterial culture object of the endotoxemia of the knot bag, excellent feeding attraction can be generated on mice, the palatability is excellent, the raw material components of the microbial agent are simple, the cost is low, and in addition, the stealing of the agents by the mice can be increased, so that the deratization efficiency is improved.
After the living bacteria culture object of the endomyzium curvatum enters the intestinal tract, the intestinal tract is anaerobic and still, and the living bacteria of the endomyzium curvatum, the faeces in the intestinal tract of the mice and the food which is not digested are fermented to generate gas, so that the gas plays a role of a propellant, the generated gas can accelerate the precipitation of barium sulfate and plays a role of blocking, so that the mice can cause acute obstruction in the intestinal tract after eating, and the mice die after being combined with heart rate failure, and the method has the advantages of short death time and high death rate.
Detailed Description
The term as used herein:
"prepared from … …" is synonymous with "comprising". The terms "comprising," "including," "having," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, step, method, article, or apparatus.
The conjunction "consisting of … …" excludes any unspecified element, step or component. If used in a claim, such phrase will cause the claim to be closed, such that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of … …" appears in a clause of the claim body, rather than immediately following the subject, it is limited to only the elements described in that clause; other elements are not excluded from the stated claims as a whole.
When an equivalent, concentration, or other value or parameter is expressed as a range, preferred range, or a range bounded by a list of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when ranges of "1 to 5" are disclosed, the described ranges should be construed to include ranges of "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a numerical range is described herein, unless otherwise indicated, the range is intended to include its endpoints and all integers and fractions within the range.
In these examples, the parts and percentages are by mass unless otherwise indicated.
"parts by mass" means a basic unit of measurement showing the mass ratio of a plurality of components, and 1 part may be any unit mass, for example, 1g may be expressed, 2.689g may be expressed, and the like. If we say that the mass part of the a component is a part and the mass part of the B component is B part, the ratio a of the mass of the a component to the mass of the B component is represented as: b. alternatively, the mass of the A component is aK, and the mass of the B component is bK (K is an arbitrary number and represents a multiple factor). It is not misunderstood that the sum of the parts by mass of all the components is not limited to 100 parts, unlike the parts by mass.
"and/or" is used to indicate that one or both of the illustrated cases may occur, e.g., a and/or B include (a and B) and (a or B).
The application provides a microbial agent, which comprises a material and Endomycetes cinnabarinus inoculated in the material, wherein the inoculation amount of the Endomycetes cinnabarinus is 5-15% of the dry weight of the material.
The microbial agent can be used as a phagostimulant, such as a phagostimulant of mice or phagostimulants of other harmful animals. The material is a solid component of a microbial agent, the material can be a material edible by mice, and meanwhile, the material is also a material for culturing the endomyxoma curcas, so that the endomyxoma curcas can be propagated in a large quantity in the material.
The strain used in the microbial inoculum is preserved in the Liaoning province microorganism strain collection center with the preservation number of LCCC70241, but the preserved strain has no special property compared with other sources of the strain of the Endomycetes. It will be appreciated that in other embodiments, the strain is not limited as long as it is a used endotoxemium.
The inclined plane strain culture conditions of the endotoxemium curvatum are as follows: the culture medium is YPD culture medium, and the culture temperature is 28-30deg.C, and the culture period is 2-3 days.
The culture conditions of the liquid strain of the Endomycetes cingulatus are as follows: the liquid culture medium is YPD culture medium, the inoculation amount is 0.5-1% by weight, the culture temperature is 28-30deg.C, and the ventilation rate is 1.1-1.6m 3 And/min, and culturing for 22-24h.
The microbial agent comprises materials and the endotoxemia of the knot bag, the microbial agent is a living bacterial culture object of the endotoxemia of the knot bag, a plurality of aromatic substances can be generated in the living bacterial culture object of the endotoxemia of the knot bag, excellent feeding attraction can be generated on mice, the palatability is excellent, the raw material components of the microbial agent are simple, the cost is low, and in addition, the stealing of the agents by the mice can be increased, so that the deratization efficiency is improved.
In a preferred embodiment, the materials comprise the following components in parts by weight: 40-80 parts of corn flour, 60-80 parts of fresh fungus chaff, 5-15 parts of bean pulp powder and 1-5 parts of ammonium sulfate.
The corn flour may be (40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 52, 53, 55, 56, 58, 60, 61, 62, 63, 65, 66, 68, 69, 70, 71, 72, 73, 75, 78, 79 or 80), for example.
The fresh mushroom bran refers to a sterile culture body obtained after edible mushrooms are prepared, for example, the sterile fresh mushroom bran obtained after flammulina velutipes and agaricus bisporus are prepared. The fresh fungus chaff may be, for example, (60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79 or 80) parts.
The soybean meal may be, for example, (5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15) parts.
Among them, for example, (1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5) parts of ammonium sulfate may be used. Ammonium sulfate is used to adjust the carbon to nitrogen ratio in the material.
Preferably, the viable count of the endotoxemia of the microbial agent is more than 2 x 10 9 CFU/g。
The application also provides a preparation method of the microbial agent, which comprises the following steps:
mixing the material and the endotoxemium cinnabarinosum according to the proportion;
controlling the thickness of the material to be 10-15cm, the water content of the material to be 45-48%, the pH of the initial material to be 6.0-6.5, the culture humidity to be 85-100%, the culture temperature to be 30-35 ℃ and the culture period to be 36-48h, thus obtaining the microbial agent.
In a preferred embodiment, after obtaining the microbial agent, the method further comprises:
and drying the microbial agent, wherein the drying temperature is not higher than 60 ℃, the drying temperature is not higher than 12% of the water content of the material, and the inactivation rate of the endotoxemium cinatum is lower than 5%.
The preservation time of the microbial agent can be prolonged by drying.
The application also provides application of the microbial agent in preparing rodenticide.
The microbial agent is used as a phagostimulant, can be mixed with other rodenticides to obtain the rodenticide, and can attract mice and kill the mice by the rodenticides. The rodenticide can be a traditional chemical rodenticide or a novel biological control rodenticide.
The application also provides a rodenticide, which comprises the microbial agent, wherein the rodenticide comprises the following components in percentage by mass: 20 to 80 percent of microbial agent, 10 to 50 percent of barium sulfate, 0.01 to 0.05 percent of diphenoxylate and 0 to 10 percent of grain.
Wherein, barium sulfate plays a role in blocking the intestinal tracts of mice, diphenoxylate plays a role in paralyzing mice, and grains and microbial agents are used for attracting the mice to eat.
After the living bacteria culture body of the endomyzium curvatum enters human intestinal tracts, the intestinal tracts are anaerobic, and the living bacteria of the endomyzium curvatum, the feces in the mouse intestinal tracts and food which is not digested are fermented to generate gas, so that the gas plays a role of a propellant, the generated gas can accelerate the precipitation of barium sulfate and plays a role of blocking, so that the mice are caused to have acute obstruction in the intestinal tracts after eating, and the mice die after being combined with heart rate failure, and the method has the advantages of short death time and high death rate.
Preferably, the rodenticide comprises: 70% of microbial agent, 30% of barium sulfate and 0.028% of diphenoxylate.
Preferably, the effective viable count in the rodenticide is greater than 4 x 10 8 CFU/g。
Embodiments of the present application will be described in detail below with reference to specific examples, but it will be understood by those skilled in the art that the following examples are only for illustration of the present application and should not be construed as limiting the scope of the present application. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1
Primary shaking culture conditions of Endomycetes cingulatus:
culture medium: YPD medium.
Liquid loading amount: 500ml triangular flask is filled with 100-120ml,8 layers of gauze is sealed.
And (3) sterilization: 1.0atm,30min.
Inoculation amount: a fungus ring.
Culture conditions: the temperature is 28-30 ℃, the speed is 160-180rpm, and the time is 18-20h.
Strain maturation standard: no mixed bacteria, the bacterial count is more than or equal to 1 x 10 8 cfu/ml。
Culturing conditions of the second-stage strain of the Endomycetes cinnabarinus:
cultivation equipment: 1m 3 A mechanically ventilated stirred tank.
Culture medium: YPD medium.
Liquid loading amount: 0.75-0.8m 3 。
Inoculation amount: 0.5-1%.
Culture conditions: the temperature is 28-30 ℃, the stirring speed is 120-160rpm, and the ventilation rate is 1.0-1.6m 3 And/min, and the time is 16-18h.
Strain maturation standard: no mixed bacteria, the bacterial count is more than or equal to 1 x 10 9 cfu/ml。
Obtaining the needed endomyzium curvatum through two-stage activation.
The endotoxem cinnabarini microbial agent of the embodiment 1 comprises a material and endotoxem cinnabarini inoculated in the material, wherein the inoculation amount of the endotoxem cinnabarini is 10% of the dry weight of the material; the material comprises the following components in parts by weight: 50 parts of corn flour, 60 parts of fresh fungus chaff, 10 parts of bean pulp powder and 2 parts of ammonium sulfate.
The preparation method of the endotoxemium cinnabarinosum microbial agent in the embodiment 1 comprises the following steps: mixing the materials and the endotoxemium according to the proportion; the thickness of the material is controlled to be 12cm, the moisture of the material is 48%, the PH of the initial material is 6.2, and the culture humidity is controlled to be: 85% for 0-24h, 95% for 25-36h, and 80% for 37-48h, and the culture temperature is controlled as follows: 0-24h at 30 ℃,25-36h at 32-35 ℃,37-48h at 32-30 ℃ and culture period at 48h, drying the cultured product at 60 ℃ to below 12% of water, pulverizing, packaging, and preserving at normal temperature to obtain the microbial agent of example 1, wherein the viable count of the product is 3.2 x 10 9 CFU/g。
The rodenticide of example 1 comprises the following components in percentage by mass: 70% of a trichoderma koningii microbial agent, 30% of barium sulfate and 0.028% of diphenoxylate. The raw materials were mixed to prepare the rodenticide of example 1.
Example 2
The endotoxem cinnabarini microbial agent of the embodiment 2 comprises a material and endotoxem cinnabarini inoculated in the material, wherein the inoculation amount of the endotoxem cinnabarini is 10% of the dry weight of the material; the material comprises the following components in parts by weight: 50 parts of corn flour, 60 parts of fresh fungus chaff, 10 parts of bean pulp powder and 1 part of ammonium sulfate.
The preparation method of the endotoxemium cinnabarinosum microbial agent in the embodiment 2 comprises the following steps: mixing the materials and the endotoxemium according to the proportion;the thickness of the material is controlled to be 12cm, the moisture of the material is 48%, the PH of the initial material is 6.2, and the culture humidity is controlled to be: 85% for 0-24h, 95% for 25-36h, and 80% for 37-48h, and the culture temperature is controlled as follows: 0-24h at 30 ℃,25-36 at 32-35 ℃,37-48h at 32-30 ℃ and culture period of 48h, drying the cultured product at 60 ℃ to below 12% of water, pulverizing, packaging, and preserving at normal temperature to obtain the microbial agent of example 2, wherein the viable count of the product is 2.5 x 10 9 CFU/g。
Comparative example 1
The rodenticide of the comparative example 1 is prepared by mixing 4% of an inactivated streptococcus faecalis culture, 60% of barium sulfate, 0.028% of diphenoxylate and 36% of grains in percentage by mass.
Test example 1 palatability test of microbial inoculant
A certain feed factory raw material workshop with higher mouse density is selected, an experimental sample A (the endomyzium curvatum microbial agent in the embodiment 1), a control sample B (corn residues) and a control sample C (bean pulp) are respectively weighed, each 100g of each bait disc is respectively put into 3 bait discs which are arranged in an equilateral triangle, 9 groups are put together, the later morning harvest is carried out, the consumption of each bait disc is counted, 4d of continuous feeding observation is carried out, the total consumption of A, B and C is calculated, the feeding rate refers to the sum of the feeding amount of one test feeding grain divided by the total feeding amount of various test feeding grains, and the calculation formula is as follows: IR (a) =a/a+b+c, and the experimental results are shown in table 1.
Table 1 palatability test results of microbial agents
From the results shown in table 1, the endotoxemium globosum microbial agent of example 1 has better palatability to mice than corn residues and soybean meal, and the endotoxemium globosum microbial agent of example 1 can be used as a phagostimulant for mice.
Test example 2 safety test of product ingredients
1. The chemical properties of the product are as follows:
a) Common names of the active ingredients: diphenonol ester
International generic name (E-ISO): diphenoxyate
Chemical name: 1- (3, 3-diphenyl-3-cyanopropyl) -4-phenyl-4-piperidinecarboxylic acid ethyl ester
The molecular formula: c (C) 30 H 32 N 2 O 2 ·HCL
Molecular weight: 489.60 (by 2007 International relative atomic mass.)
Biological activity: enteroparalysis;
b) Common names of active ingredients: barium sulfate
ISO common name: barilum sulfate
Experimental formula: baSO (Baso) 4
Relative molecular mass: 233.40 (by 2007 International relative atomic mass.)
Traits: the product is white loose fine powder, odorless, tasteless and nontoxic.
Biological activity: a blocking agent;
c) Common names of active ingredients: microbial agent
Generic name: endomycetes (Endomycopsis fibuligera), also known as Saccharomycetes, complex film yeast (Saccharomycopsis fibuligera),
traits: the product is light yellow-brown fine powder, and has peculiar smell.
Biological activity: living organism and mouse food.
2. Toxicology of the product
The applicant entrusted to the toxicology experiments of the products performed in the university of chinese pharmacopoeia pharmacological research laboratory to verify the safety thereof, and the results are shown in the following tables 2 and 3.
1. Diphenoxylate toxicology test
TABLE 2 Phenoxolone ester toxicology test
2. Barium sulfate toxicology test
TABLE 3 barium sulfate toxicology test
3. Safety description of microbial agent
Endomycetes bursae (Endomycopsis fibuligera), also known as Saccharomycetes bursae complex film yeasts (Saccharomycopsis fibuligera), are capable of expressing enzymes that produce starch liquefying enzymes and saccharifying enzymes, and are considered to be one of the best ascomycetes that produce amylolytic enzymes. The microbial agent is widely applied to the fields of brewing industry, enzyme preparation production, live bacteria preparation for feeding and the like in the world at present, and has high safety, and the safety grade is a type of microorganism.
In conclusion, the related components of the product have higher safety.
Test example 3 field efficacy test for controlling farmland mice
Test crop: farmland, corn, soybean, etc
Control object: rat pest
Test agent: example 1 and comparative example 1 rodenticides
The application method comprises the following steps: saturated bait casting device
Period and amount of administration: in the initial stage of the propagation of the mice, the dosage of 500.5 g/hectare of the active ingredient is preferable from 3 middle ten days to 4 middle ten days of spring.
Test results:
example 1 rodenticide, comparative example 1 rodenticide and wheat without drugs were each provided with 50 feeding points, and continuously investigated for 3d with a piracy rate of 70.00%, 36.00% and 75.40%, respectively, (piracy rate= bait consumption number/investigation bait number); the piracy rates were 64.80%, 29.20% and 69.20%, respectively (piracy rate = bait consumption heap/investigation bait count); example 1 rodenticide feeding coefficient of 0.94, (rodenticide feeding coefficient=consumption of poison bait/consumption of non-toxic bait), comparative example 1 feeding coefficient of 0.42 (greater than 0.3); the two rodenticides of the example 1 and the comparative example 1 are shown to have better palatability to the harmful mice, and the palatability of the rodenticide of the example 1 to the harmful mice is obviously better than that of the rodenticide of the comparative example 1.
The field control results are shown in table 4, and according to table 4, the rodenticides of example 1 and comparative example 1 have rodenticide effects of 83.39% and 68.32% after 30d, and the corrected rodenticide effects are 82.40% and 66.42%, respectively, which indicates that the two rodenticides have better control effects on farmland mice, the effect of example 1 is obviously higher than that of comparative example 1, and meanwhile, no toxic effect on human, animals and plants is found in the experimental process, so that the environment is safe.
Table 4 field control results
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present application.
Furthermore, those skilled in the art will appreciate that while some embodiments herein include some features but not others included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the present application and form different embodiments. For example, in the claims below, any of the claimed embodiments may be used in any combination. The information disclosed in this background section is only for enhancement of understanding of the general background of the application and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Claims (4)
1. The microbial rodenticide is characterized by comprising the following components in percentage by mass: 20-80% of microbial agent, 10-50% of barium sulfate and 0.01-0.05% of diphenoxylate;
the microbial agent comprises a material and the endotoxemia of the knot bag inoculated in the material, wherein the inoculation amount of the endotoxemia of the knot bag is 5-15% of the dry weight of the material;
the material comprises the following components in parts by weight: 40-80 parts of corn flour, 60-80 parts of fresh fungus chaff, 5-15 parts of bean pulp powder and 1-5 parts of ammonium sulfate;
the viable count of the Endomycopsis grossedentata in the microbial agent is more than 2 multiplied by 10 9 CFU/g；
The effective viable count in the microbial rodenticide is greater than 4 multiplied by 10 8 CFU/g。
2. The microbial rodenticide according to claim 1, wherein the fresh mushroom bran comprises a sterile culture obtained after preparation of edible mushrooms.
3. The microbial rodenticide according to claim 1 or 2, wherein the preparation method of the microbial inoculant comprises:
mixing the material and the endotoxemium cinnabarinosum according to the proportion;
controlling the thickness of the material to be 10-15cm, the moisture of the material to be 45-48%, the pH of the initial material to be 6.0-6.5, the culture humidity to be 85-100%, the culture temperature to be 30-35 ℃ and the culture period to be 36-48h, thus obtaining the microbial agent.
4. The microbial rodenticide according to claim 3, wherein after obtaining the microbial inoculant, further comprising:
and drying the microbial agent, wherein the drying temperature is not higher than 60 ℃, the drying temperature is not higher than 12% of the water content of the material, and the inactivation rate of the endotoxemium cinatum is lower than 5%.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB925776A (en) * | 1960-03-17 | 1963-05-08 | Svenska Sockerfabriks Ab | Preparation of food yeast and apparatus therefor |
| CN1352879A (en) * | 2001-11-27 | 2002-06-12 | 朱天相 | Biochemical rodenticide |
| CN1543799A (en) * | 2003-11-21 | 2004-11-10 | 黄建忠 | Biochemical raticide |
| AU2007216693A1 (en) * | 2001-03-01 | 2007-09-27 | Ultra Biotech Limited | Biological fertilizer compositions comprising manure, sludge or garbage |
| MY134845A (en) * | 2000-09-05 | 2007-12-31 | Ultra Biotech Ltd | A biological fertilizer based on yeasts |
| CN110476984A (en) * | 2019-09-16 | 2019-11-22 | 四川鑫鑫骄扬生物科技有限公司 | Application of the high-hydroscopicity bamboo fibre polysaccharide in preparation mouse intestinal obstruction agent |
| CN110973123A (en) * | 2019-12-30 | 2020-04-10 | 山西省植物保护植物检疫总站 | Non-raw grain deratization bait with good palatability and preparation method thereof |
-
2022
- 2022-06-28 CN CN202210751580.2A patent/CN115005235B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB925776A (en) * | 1960-03-17 | 1963-05-08 | Svenska Sockerfabriks Ab | Preparation of food yeast and apparatus therefor |
| MY134845A (en) * | 2000-09-05 | 2007-12-31 | Ultra Biotech Ltd | A biological fertilizer based on yeasts |
| AU2007216693A1 (en) * | 2001-03-01 | 2007-09-27 | Ultra Biotech Limited | Biological fertilizer compositions comprising manure, sludge or garbage |
| CN1352879A (en) * | 2001-11-27 | 2002-06-12 | 朱天相 | Biochemical rodenticide |
| CN1543799A (en) * | 2003-11-21 | 2004-11-10 | 黄建忠 | Biochemical raticide |
| CN110476984A (en) * | 2019-09-16 | 2019-11-22 | 四川鑫鑫骄扬生物科技有限公司 | Application of the high-hydroscopicity bamboo fibre polysaccharide in preparation mouse intestinal obstruction agent |
| CN110973123A (en) * | 2019-12-30 | 2020-04-10 | 山西省植物保护植物检疫总站 | Non-raw grain deratization bait with good palatability and preparation method thereof |
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