CN115005235A - Microbial rodenticide - Google Patents
Microbial rodenticide Download PDFInfo
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- CN115005235A CN115005235A CN202210751580.2A CN202210751580A CN115005235A CN 115005235 A CN115005235 A CN 115005235A CN 202210751580 A CN202210751580 A CN 202210751580A CN 115005235 A CN115005235 A CN 115005235A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 63
- 239000003128 rodenticide Substances 0.000 title claims abstract description 31
- 239000000463 material Substances 0.000 claims abstract description 54
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 45
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 claims abstract description 28
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims description 15
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 14
- HYPPXZBJBPSRLK-UHFFFAOYSA-N diphenoxylate Chemical compound C1CC(C(=O)OCC)(C=2C=CC=CC=2)CCN1CCC(C#N)(C=1C=CC=CC=1)C1=CC=CC=C1 HYPPXZBJBPSRLK-UHFFFAOYSA-N 0.000 claims description 12
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- 238000000034 method Methods 0.000 claims description 11
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- 235000019764 Soybean Meal Nutrition 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 8
- 239000004455 soybean meal Substances 0.000 claims description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 7
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 7
- 235000013339 cereals Nutrition 0.000 claims description 7
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- 238000002156 mixing Methods 0.000 claims description 6
- 241000862992 Chondromyces Species 0.000 claims description 5
- 241000221960 Neurospora Species 0.000 claims description 4
- 230000002779 inactivation Effects 0.000 claims description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 11
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 abstract description 10
- 235000013305 food Nutrition 0.000 abstract description 10
- 235000019629 palatability Nutrition 0.000 abstract description 9
- 239000000126 substance Substances 0.000 abstract description 8
- 239000002994 raw material Substances 0.000 abstract description 7
- 241001085826 Sporotrichum Species 0.000 abstract description 6
- 239000000203 mixture Substances 0.000 abstract description 6
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- 206010019280 Heart failures Diseases 0.000 abstract description 3
- 206010061876 Obstruction Diseases 0.000 abstract description 3
- 230000001154 acute effect Effects 0.000 abstract description 3
- 125000003118 aryl group Chemical group 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 239000003380 propellant Substances 0.000 abstract description 3
- 241000233866 Fungi Species 0.000 abstract description 2
- 241000700159 Rattus Species 0.000 description 13
- 241000699666 Mus <mouse, genus> Species 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 9
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- 241000699670 Mus sp. Species 0.000 description 4
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- 244000251953 Agaricus brunnescens Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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- 235000019779 Rapeseed Meal Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
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- 239000002981 blocking agent Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/004—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits rodenticidal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P11/00—Rodenticides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
- Food Science & Technology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The application provides a microorganism rodenticide, relates to the biological control field, including the microbial inoculum, this microbial inoculum includes material and knot bag paecilomyces, and this microbial inoculum is the living fungus culture object of knot bag paecilomyces, can produce a lot of aromatic substance, can produce splendid food calling effect to the mouse, and the palatability is good, and this microbial inoculum raw materials composition is simple, and is with low costs, can increase the stealing of mouse to the medicament moreover and eat, therefore has improved the efficiency of deratization. The microbial agent is used as a phagostimulant to prepare the rodenticide, after the live bacteria culture object of the sporotrichum capsulatum enters the intestinal tract, because the intestinal tract is in an anaerobic environment, the live bacteria of the sporotrichum capsulatum, excrement in the intestinal tract of the rat and undigested food are fermented to generate gas which plays a role of a propellant, the generated gas can accelerate barium sulfate to precipitate and play a role in blocking, so that the rat can cause acute obstruction of the intestinal tract after eating, and the rat can die due to heart failure, the death time is short, and the death rate is high.
Description
Technical Field
The application relates to the field of biological control, in particular to a microbial rodenticide.
Background
Most of the existing phagostimulants added into rodenticide are made from sugar, protein and fat, and then spice substances such as spices, aromatics and lipids or chemical components capable of playing a food calling role, such as glucose, sucrose, corn flour, wheat bran, bean pulp, rapeseed meal, fish meal, various fats, yeast and beef extract, are added.
The prior murine phagostimulant has the following obvious characteristics: 1. the raw material composition is complex, the product control difficulty is high, and the industrial preparation is not facilitated. 2. The preparation cost is high. 3. The preparation process does not follow the habits of animals, but more according to the preferences of human beings, and does not achieve better food calling effect. 4. The mouse bait is not well combined with food, so that the mouse can be attracted from a distance, and the product can achieve the due effect only by having good palatability.
Disclosure of Invention
The application aims to provide a microbial rodenticide, and aims to solve the problems of complex raw materials, high product control difficulty, high preparation cost and poor deratization effect of the existing rodent phagostimulant.
In order to achieve the above object, the present application provides a microbial rodenticide, which comprises, by mass: 20-80% of microbial agent, 10-50% of barium sulfate, 0.01-0.05% of diphenoxylate and 0-10% of grain.
Preferably, the microbial agent comprises a material and the endosporidium capsulatum inoculated in the material, and the inoculation amount of the endosporidium capsulatum is 5-15% of the dry weight of the material.
Preferably, the materials comprise the following components in parts by weight: 40-80 parts of corn flour, 60-80 parts of fresh mushroom bran, 5-15 parts of soybean meal and 1-5 parts of ammonium sulfate.
Preferably, the fresh mushroom bran comprises a non-contaminated culture obtained after preparation of the edible mushroom.
Preferably, the number of viable bacteria of the endospora capsulata in the microbial agent is greater than 2 x 10 9 CFU/g。
Preferably, the preparation method of the microbial agent comprises the following steps:
mixing the material and the capsulopsis according to the proportion;
controlling the thickness of the material to be 10-15cm, the water content of the material to be 45-48%, the pH of the initial material to be 6.0-6.5, the culture humidity to be 85-100%, the culture temperature to be 30-35 ℃, and the culture period to be 36-48h, thereby obtaining the microbial inoculum.
Preferably, after obtaining the microbial agent, the method further comprises:
and drying the microbial agent at the drying temperature of no more than 60 ℃ until the water content of the material is less than 12%, wherein the inactivation rate of the endosporidium polycephalum is less than 5%.
Preferably, the effective viable count in the microbial rodenticide is greater than 4 x 10 8 CFU/g。
Compared with the prior art, the beneficial effect of this application includes:
the application provides a microbial inoculum includes material and knot bag paecilomyces, and this microbial inoculum is the live bacteria culture object of knot bag paecilomyces, can produce a lot of aromatic class material in this live bacteria culture object of knot bag paecilomyces, can produce splendid food calling effect to the mouse, and the palatability is good, and this microbial inoculum raw materials composition is simple, and is with low costs, can increase the stealing of mouse to the medicament and eat moreover, therefore has improved the efficiency of killing rats.
The microbial agent is used as a phagostimulant to prepare the rodenticide, after the live bacteria culture object of the sporotrichum capsulatum enters the intestinal tract, because the intestinal tract is in an anaerobic environment, the live bacteria of the sporotrichum capsulatum, excrement in the intestinal tract of the rat and undigested food are fermented to generate gas which plays a role of a propellant, the generated gas can accelerate barium sulfate to precipitate and play a role in blocking, so that the rat can cause acute obstruction of the intestinal tract after eating, and the rat can die due to heart failure, the death time is short, and the death rate is high.
Detailed Description
The term as used herein:
"prepared from … …" is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
The conjunction "consisting of … …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of … …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when the range "1 ~ 5" is disclosed, the ranges described should be construed to include the ranges "1 ~ 4", "1 ~ 3", "1 ~ 2 and 4 ~ 5", "1 ~ 3 and 5", and the like. When a range of values is described herein, unless otherwise specified, the range is intended to include the endpoints thereof, and all integers and fractions within the range.
In the examples, the parts and percentages are by mass unless otherwise indicated.
"part by mass" means a basic unit of measure indicating a mass ratio of a plurality of components, and 1 part may represent any unit mass, for example, 1g or 2.689 g. If we say that the part by mass of the component A is a part by mass and the part by mass of the component B is B part by mass, the ratio of the part by mass of the component A to the part by mass of the component B is a: b. alternatively, the mass of the A component is aK and the mass of the B component is bK (K is an arbitrary number, and represents a multiple factor). It is unmistakable that, unlike the parts by mass, the sum of the parts by mass of all the components is not limited to 100 parts.
"and/or" is used to indicate that one or both of the illustrated conditions may occur, e.g., a and/or B includes (a and B) and (a or B).
The application provides a microbial agent, which comprises a material and an endoperosporium capsulatum inoculated in the material, wherein the inoculation amount of the endoperosporium capsulatum is 5-15% of the dry weight of the material.
Wherein the microorganism bacterium agent is used as a phagostimulant, such as a phagostimulant for rats or a phagostimulant for other harmful animals. The material is solid component of microbial agent, and can be edible substance for mouse, and simultaneously the material is also substance for culturing the paecilomyces capsulatus, and the paecilomyces capsulatus can propagate in mass in the material.
The strain endosporidium Fulvioides used in the microbial agent of the application is preserved in Liaoning province microbial strain preservation center with the preservation number of LCCC70241, but the preserved strain does not have special properties compared with other sources of endosporidium Fulvioides. It is to be understood that in other examples, any strain of Neurospora capsulata may be used, and the strain is not limited thereto.
The slant culture conditions of the echinococcus capsulatus are as follows: the culture medium is YPD culture medium, the culture temperature is 28-30 deg.C, and the culture period is 2-3 days.
The culture conditions of the liquid strain of the ascochyta is as follows: the liquid culture medium is YPD culture medium with inoculation amount of 0.5-1 wt%, culture temperature of 28-30 deg.C, and ventilation amount of 1.1-1.6m 3 Min, and the culture period is 22-24 h.
The application provides a microbial inoculum includes material and knot bag paecilomyces, and this microbial inoculum is the live bacteria culture object of knot bag paecilomyces, can produce a lot of aromatic class material in this live bacteria culture object of knot bag paecilomyces, can produce splendid food calling effect to the mouse, and the palatability is good, and this microbial inoculum raw materials composition is simple, and is with low costs, can increase the stealing of mouse to the medicament and eat moreover, therefore has improved the efficiency of killing rats.
In a preferred embodiment, the material comprises the following components in parts by weight: 40-80 parts of corn flour, 60-80 parts of fresh mushroom bran, 5-15 parts of soybean meal and 1-5 parts of ammonium sulfate.
The corn flour may be (40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 52, 53, 55, 56, 58, 60, 61, 62, 63, 65, 66, 68, 69, 70, 71, 72, 73, 75, 78, 79, or 80), for example.
Wherein the fresh mushroom bran refers to a culture obtained after preparation of edible mushrooms, such as fresh mushroom bran obtained after preparation of needle mushrooms and agaricus bisporus. The fresh mushroom bran may be, for example, (60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79 or 80) parts.
The amount of the soybean meal may be, for example, (5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15) parts.
The ammonium sulfate may be, for example, (1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 or 5) parts. Ammonium sulfate is used to adjust the carbon to nitrogen ratio in the material.
Preferably, the number of viable bacteria of the endospora capsulata in the microbial agent is greater than 2 x 10 9 CFU/g。
The application also provides a preparation method of the microbial agent, which comprises the following steps:
mixing the material and the capsulopsis according to the proportion;
controlling the thickness of the material to be 10-15cm, the water content of the material to be 45-48%, the pH of the initial material to be 6.0-6.5, the culture humidity to be 85-100%, the culture temperature to be 30-35 ℃ and the culture period to be 36-48h, thus obtaining the microbial agent.
In a preferred embodiment, after obtaining the microbial agent, the method further comprises:
and drying the microbial agent at the drying temperature of no more than 60 ℃ until the water content of the material is less than 12%, wherein the inactivation rate of the endosporidium polycephalum is less than 5%.
The preservation time of the microbial agent can be prolonged by drying.
The application also provides an application of the microbial agent in preparation of a rodenticide.
The microbial agent is used as a phagostimulant and can be mixed with other rodenticides to obtain the rodenticide, the microbial agent lures mice, and the rodenticides kill the mice. The raticide can be a traditional chemical raticide or a novel biological control type raticide.
The application also provides a rodenticide, which comprises the microbial agent, and the rodenticide comprises the following components in percentage by mass: 20 to 80 percent of microbial agent, 10 to 50 percent of barium sulfate, 0.01 to 0.05 percent of diphenoxylate and 0 to 10 percent of grain.
Wherein, barium sulfate plays a role in blocking the intestinal tract of the mouse, diphenoxylate plays a role in paralyzing the mouse, and the grain and microbial agent are used for luring the mouse to eat.
The microbial agent is used as a phagostimulant to prepare the rodenticide, after the live bacteria culture object of the sporotrichum capsulatum enters the intestinal tract of a human, because the intestinal tract is an anaerobic environment, the live bacteria of the sporotrichum capsulatum, excrement in the intestinal tract of the rat and undigested food are fermented to generate gas which plays a role of a propellant, the generated gas can accelerate barium sulfate to precipitate and play a role in blocking, so that the rat can cause acute obstruction of the intestinal tract after eating, and the rat can die due to heart failure, the death time is short, and the death rate is high.
Preferably, the rodenticide comprises: 70% of microbial agent, 30% of barium sulfate and 0.028% of diphenoxylate.
Preferably, the number of active bacteria in the rodenticide is greater than 4 x 10 8 CFU/g。
Embodiments of the present application will be described in detail below with reference to specific examples, but those skilled in the art will appreciate that the following examples are only illustrative of the present application and should not be construed as limiting the scope of the present application. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Example 1
The first-stage shaking table strain culture conditions of the Neurospora capsulata comprise:
culture medium: YPD medium.
Liquid loading amount: 120ml in a 500ml triangular bottle, and sealed by 8 layers of gauze.
And (3) sterilization: 1.0atm, 30 min.
Inoculation amount: a bacteria ring.
The culture conditions are as follows: the temperature is 28-30 ℃, the speed is 160-180rpm, and the time is 18-20 h.
And (3) strain maturation standard: no mixed bacteria, the number of bacteria is more than or equal to 1 x 10 8 cfu/ml。
Culturing conditions of the secondary strain of the echinospora capsulata:
the culture equipment comprises: 1m 3 The culture tank is stirred by mechanical ventilation.
Culture medium: YPD medium.
Liquid loading amount: 0.75-0.8m 3 。
Inoculation amount: 0.5 to 1 percent.
The culture conditions are as follows: the temperature is 28-30 ℃, the stirring speed is 120- 3 Min, time 16-18 h.
And (3) strain maturation standard: no mixed bacteria, the number of bacteria is more than or equal to 1 x 10 9 cfu/ml。
And (5) performing two-stage activation to obtain the required ascochyta.
The endoplasmic reticulum-buckled-bag microbial agent of the embodiment 1 comprises a material and the endoplasmic reticulum-buckled-bag inoculated in the material, wherein the inoculation amount of the endoplasmic reticulum-buckled-bag is 10% of the dry weight of the material; the materials comprise the following components in parts by weight: 50 parts of corn flour, 60 parts of fresh fungus bran, 10 parts of soybean meal powder and 2 parts of ammonium sulfate.
The method for preparing an endosporidium parvulum microbial agent of embodiment 1 includes: mixing the materials and the endosporidium polycephalum according to the proportion; the thickness of the material is controlled to be 12cm, the water content of the material is controlled to be 48%, the PH of the initial material is 6.2, and the culture humidity is controlled to be: 85% in 0-24h, 95% in 25-36h and 80% in 37-48h, and controlling the culture temperature as follows: 0-24h at 30 deg.C, 25-36h at 32-35 deg.C, 37-48h at 32-30 deg.C, culturing for 48h, and drying the cultured product at 60 deg.CPulverizing, packaging, and storing at room temperature to obtain the microbial preparation of example 1 with viable count of 3.2 x 10 9 CFU/g。
The rodenticide of example 1 comprises, in mass percent: 70% of endosporidium-fibuligera microbial agent, 30% of barium sulfate and 0.028% of diphenoxylate. The raw materials were mixed to prepare the rodenticide of example 1.
Example 2
The endoplasmic reticulum-buckled-bag microbial agent of the embodiment 2 comprises a material and the endoplasmic reticulum-buckled-bag inoculated in the material, wherein the inoculation amount of the endoplasmic reticulum-buckled-bag is 10% of the dry weight of the material; the materials comprise the following components in parts by weight: 50 parts of corn flour, 60 parts of fresh mushroom bran, 10 parts of soybean meal powder and 1 part of ammonium sulfate.
The method for preparing an endosporidium parvulum microbial agent of embodiment 2 includes: mixing the materials with the capsulopsis according to the proportion; the thickness of the material is controlled to be 12cm, the water content of the material is controlled to be 48%, the PH of the initial material is 6.2, and the culture humidity is controlled to be: 85% in 0-24h, 95% in 25-36h and 80% in 37-48h, and controlling the culture temperature as follows: 0-24h at 30 deg.C, 25-36 at 32-35 deg.C, 37-48h at 32-30 deg.C, culturing for 48h, drying the cultured product at 60 deg.C to below 12% water, pulverizing, packaging, and storing at room temperature to obtain the microbial preparation of example 2 with viable count of 2.5 × 10 9 CFU/g。
Comparative example 1
Taking 4% of inactivated streptococcus faecalis culture, 60% of barium sulfate, 0.028% of diphenoxylate and 36% of grain by mass percent, and mixing the above materials to prepare the raticide in the comparative example 1.
Test example 1 palatability testing of microbial Agents
Selecting a certain feed factory raw material workshop with higher mouse density, respectively weighing an experimental sample A (the bag-buckled endosporidium-simulating microbial agent in the embodiment 1), a control sample B (corn grit) and a control sample C (soybean meal), respectively putting each bait tray 100g each time into 3 bait trays arranged in an equilateral triangle, putting 9 groups in total, collecting every morning, counting the consumption of each bait tray, continuously putting and observing for 4d, calculating the respective total consumption of A, B and C, and calculating the ingestion rate, wherein the ingestion rate refers to the total ingestion of one test feeding grain divided by the total ingestion of each test feeding grain, and the calculation formula is as follows: ir (a) ═ a/a + B + C, and the results are shown in table 1.
TABLE 1 palatability test results of microbial Agents
From the results in table 1, it is understood that the endophytic fungi-button capsule microbial agent of example 1 has better palatability to the mice than corn steep liquor and soybean meal, and the endophytic fungi-button capsule microbial agent of example 1 can be used as a feeding attractant for the mice.
Test example 2 safety test of ingredients of product
Firstly, the chemical properties of the product are as follows:
a) common name of active ingredients: diphenoxylate
International common name (E-ISO): diphenoxylate
Chemical name: 1- (3, 3-Diphenyl-3-cyanopropyl) -4-phenyl-4-piperidinecarboxylic acid ethyl ester
The molecular formula is as follows: c 30 H 32 N 2 O 2 ·HCL
Molecular weight: 489.60 (according to 2007 international relative atomic mass meter)
And (3) biological activity: enteroparalysis;
b) common names of active ingredients: barium sulfate
ISO common name: barium sulfate
Experimental formula (II): BaSO 4
Relative molecular mass: 233.40 (according to 2007 international relative atomic mass meter)
The characteristics are as follows: the product is white loose fine powder, and has no odor, odor and toxicity.
And (3) biological activity: a blocking agent;
c) common names of active ingredients: microbial agent
General name: neurospora species (Endomycopsis fibuligera), also known as Saccharomyces cerevisiae,
the characteristics are as follows: the product is light yellow-brown fine powder, and has special smell.
And (3) biological activity: biological live bacteria, and rodent food.
II, toxicology of product
The applicant entrusted the pharmaceutical teaching and research laboratory of the university of chinese pharmacy with toxicological experiments to verify the safety of the product, and the results are shown in tables 2 and 3 below.
1. Toxicology test of diphenoxylate
TABLE 2 toxicology test of diphenoxylate
2. Toxicology test of barium sulfate
TABLE 3 toxicological test of barium sulfate
3. Microbial agent safety Specification
Endomycopsis fibuligera (Endomycopsis fibuligera), also known as Saccharomycopsis fibuligera (Saccharomyces fibuligera), is one of the best ascomycete yeasts expressing starch liquefying and saccharifying enzymes and producing amylolytic enzymes. The microbial composite microbial agent is widely applied to the fields of brewing industry, enzyme preparation production, feed live bacteria preparation and the like internationally, has a safety level of one type of microorganism, and has high safety.
In conclusion, the related components of the product have higher safety.
Test example 3 field efficacy test for controlling farmland rats
And (3) test crops: farmland, maize, soybean, etc
The control object is: injurious mouse
Reagent to be tested: rodenticide of example 1 and comparative example 1
The application method comprises the following steps: saturated bait throwing
The application period and dosage are as follows: in the early period of the murine reproduction, from 3 to 4 in the middle of the spring, the dosage is preferably 500.5 g/ha of active ingredient.
And (3) test results:
example 1 raticide, comparative example 1 raticide and unpainted wheat were provided with 50 feeding points, respectively, and continuously investigated for 3d, and the stealing rate was 70.00%, 36.00% and 75.40%, respectively (stealing rate ═ bait consumption pile number/investigation bait pile number); the predation rates were 64.80%, 29.20%, and 69.20%, respectively (predation rate: bait consumption amount/investigational bait amount); example 1 a raticide feeding coefficient of 0.94, (raticide feeding coefficient ═ bait consumption/no-bait consumption), comparative example 1 a raticide feeding coefficient of 0.42 (greater than 0.3); the results show that the raticide of example 1 and comparative example 1 has better palatability to rats, and the raticide of example 1 has obviously better palatability to rats than the raticide of comparative example 1.
The field control effect results are shown in table 4, and it can be seen from table 4 that the deratization effects of the raticides of example 1 and comparative example 1 are 83.39% and 68.32% respectively after 30 days, and the corrected deratization effects are 82.40% and 66.42% respectively, which shows that the two raticides have better control effects on the farmland pest rats, the effect of example 1 is obviously higher than that of comparative example 1, and no toxic action on human, animals and plants is found in the experimental process, so that the raticide is safe to the environment.
TABLE 4 field control results
Finally, it should be noted that: the above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.
Moreover, those skilled in the art will appreciate that while some embodiments herein include some features included in other embodiments, rather than other features, combinations of features of different embodiments are meant to be within the scope of the application and form different embodiments. For example, in the claims above, any of the claimed embodiments may be used in any combination. The information disclosed in this background section is only for enhancement of understanding of the general background of the application and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Claims (8)
1. A microbial rodenticide, which is characterized by comprising the following components in percentage by mass: 20-80% of microbial agent, 10-50% of barium sulfate, 0.01-0.05% of diphenoxylate and 0-10% of grain.
2. The microbial rodenticide according to claim 1, wherein the microbial agent comprises a material and an endophytic fungi-button sac inoculated in the material, and the inoculation amount of the endophytic fungi-button sac is 5-15% of the dry weight of the material.
3. The microbial rodenticide according to claim 2, wherein the material comprises, in parts by weight: 40-80 parts of corn flour, 60-80 parts of fresh mushroom bran, 5-15 parts of soybean meal and 1-5 parts of ammonium sulfate.
4. The microbial rodenticide according to claim 3, wherein the fresh mushroom bran comprises a sterile culture obtained after preparation of the edible mushroom.
5. The microbial rodenticide according to claim 2, wherein the number of viable bacteria of the Neurospora capsulata in the microbial agent is greater than 2 x 10 9 CFU/g。
6. The microbial rodenticide according to any one of claims 2 to 5, wherein the microbial agent is prepared by a method comprising:
mixing the material and the endosporidium polycephalum according to a ratio;
controlling the thickness of the material to be 10-15cm, the water content of the material to be 45-48%, the pH of the initial material to be 6.0-6.5, the culture humidity to be 85-100%, the culture temperature to be 30-35 ℃, and the culture period to be 36-48h, thereby obtaining the microbial inoculum.
7. The microbial rodenticide according to claim 6, further comprising, after obtaining the microbial agent:
and drying the microbial agent at the drying temperature of no more than 60 ℃ until the water content of the material is less than 12%, wherein the inactivation rate of the endosporidium polycephalum is less than 5%.
8. A microbial rodenticide according to any one of claims 2 to 5, wherein the effective number of viable bacteria in the microbial rodenticide is greater than 4 x 10 8 CFU/g。
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