CN110463513A - A kind of culture medium for cultivating and cultural method of selenium-rich agaric - Google Patents
A kind of culture medium for cultivating and cultural method of selenium-rich agaric Download PDFInfo
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- CN110463513A CN110463513A CN201910871495.8A CN201910871495A CN110463513A CN 110463513 A CN110463513 A CN 110463513A CN 201910871495 A CN201910871495 A CN 201910871495A CN 110463513 A CN110463513 A CN 110463513A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The present invention provides the culture medium for cultivating and cultural method of a kind of selenium-rich agaric, belong to field of biotechnology.First aspect, the present invention provides a kind of culture medium for cultivating of selenium-rich agaric, culture medium for cultivating includes that agaric extracts slag, sawdust, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast, further includes producing lignin-degrading enzymes fungi and cellulase-producing fungi；Second aspect, the present invention also provides a kind of cultural methods of selenium-rich agaric.The agaric fructification and selenium-rich level cultivated using selenium-rich fungus cultivation culture medium of the present invention compared with prior art, are significantly increased.
Description
Technical field
The present invention relates to field of biotechnology, culture medium for cultivating and cultivation side in particular to a kind of selenium-rich agaric
Method.
Background technique
Selenium is mainly used for selenium deficiency patient and endemic illness in the application of health field --- the prevention and treatment of Keshan disease, and
The selenium deficiency patient maintained for a long time by intravenous hyperalimentation.Since inorganic selenium salt is more toxic, Japan has forbidden eating for 1993
It adds, is supported using organic forms selenium or Se-rich farm products such as the higher Selenonic protein of safety, amino acid in product and feed.
Scientific circles are the study found that the height of blood selenium level and the generation of cancer are closely bound up.A large amount of survey data explanation, one
The height of Se content has direct relation with the disease incidence of cancer in a area food and soil, such as: the food and soil in this area
Se content in earth is high, and the morbidity and mortality of cancer are with regard to low, conversely, the cancer morbidity and the death rate in this area are just
Height, the fact illustrate the generation close relation of selenium and cancer.Scientific circles also identify selenium with pre- anti-cancer effect simultaneously, are
" king of anti-cancer " of body trace element.
Ya Lishengna university, U.S. Cancer center professor Clark carries out 13 years check experiments to 1312 cancer patients.Knot
Fruit shows daily 200 μ g of selenium-supply, and cancer mortality decline 50%, cancer total incidence decline 37%, wherein lung cancer declines 46%,
Intestinal cancer decline 58%, prostate cancer decline 63%.2003 Food and Drug Adminstration of the US (FDA) express: " selenium, which can reduce, suffers from cancer
Risk " and " selenium can generate anticancer in human body and be changed into use ".
Selenium-enriched food is exactly the food rich in trace elements of selenium.It is generally divided into natural selenium-rich food (also known as vegetable active selenium
Food), external source selenium selenium-enriched food (also referred to as artificial organic selenium food).
In conclusion " selenium " is needed by human, and have no control over oneself, therefore 200 μ of supplement daily suggest in the World Health Organization
G selenium, can effectively prevention of various diseases it is high-incidence.Worldwide nutrition scholar, biochemical society chairman, Ba Boya roller doctor claim: "
Selenium " is to extend service life most important mineral nutrient, is embodied in its comprehensive protection to human body, we should not be when sick
Just expect it.
Recent domestic can be such that inorganic selenium absorbs studies have shown that edible mushroom has stronger selenium rich ability by fructification
Conversion is enriched in fructification.
Black fungus is famous mountain delicacy, it is edible, can medicine, can mend, on Chinese common people's dining table long food do not mind, have " element in it
The good reputation of meat or fish " is referred to as " the black rarity in Chinese meal " in the world.Polysaccharide physical efficiency in agaric decomposes tumour, so can mention
The immunity of high people has good antitumaous effect.And this polysaccharide body also has dredging vascellum, removes cholesterol in blood vessel
Effect, it is possible to which hypoglycemic, reducing blood lipid prevents thrombosis, prevents cranial vascular disease.Also there is agaric nourishing to moisten
Dry, the beneficial stomach of blood-nourishing food therapy effect, thus be various modern civilization diseases for example hypertension, heart disease, cancer patient health food.
In consideration of it, the present invention provide it is a kind of using agaric extract slag, sawdust, rice chaff, wheat bran skin, bean powder, lime, gypsum,
Broken wall Se-enriched yeast prepares culture medium for cultivating, and the cultural method of production selenium-rich agaric fructification.
Summary of the invention
The purpose of the present invention is to provide the culture medium for cultivating and cultural method of a kind of selenium-rich agaric.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
In a first aspect, the present invention provides a kind of culture medium for cultivating of selenium-rich agaric, culture medium for cultivating include agaric extract slag,
Sawdust, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast.
Further, in preferred embodiments of the present invention, according to parts by weight, it is 1~5 part, sawdust that agaric, which extracts slag,
75~80 parts, 5~8 parts of rice chaff, 3~6 parts of wheat bran skin, bean powder is 2~3 parts, 0.2~0.8 part of lime, 0.2~0.8 part of gypsum and
Broken wall Se-enriched yeast is 1~3 part.
Further, in preferred embodiments of the present invention, according to parts by weight, it is 3 parts, sawdust 80 that agaric, which extracts slag,
Part, 8 parts of rice chaff, 3 parts of wheat bran skin, bean powder are 3 parts, 0.5 part of lime, 0.5 part of gypsum and broken wall Se-enriched yeast are 2 parts.
Further, in preferred embodiments of the present invention, according to parts by weight, culture medium for cultivating further includes producing lignin
Degrading enzyme fungi and cellulase-producing fungi.
Further, in preferred embodiments of the present invention, according to parts by weight, it is 1~5 part, sawdust that agaric, which extracts slag,
75~80 parts, 5~8 parts of rice chaff, 3~6 parts of wheat bran skin, bean powder be 2~3 parts, 0.2~0.8 part of lime, 0.2~0.8 part of gypsum,
Broken wall Se-enriched yeast is 1~3 part, produces 0.1~0.3 part of lignin-degrading enzymes fungi and/or cellulase-producing fungi liquid seeds
0.1~0.3 part.
Further, in preferred embodiments of the present invention, according to parts by weight, it is 3 parts, sawdust 80 that agaric, which extracts slag,
Part, 8 parts of rice chaff, 3 parts of wheat bran skin, bean powder are 3 parts, 0.5 part of lime, 0.5 part of gypsum, broken wall Se-enriched yeast are 2 parts, produce lignin
0.2 part of degrading enzyme fungi and cellulase-producing fungi are 0.2 part.
Second aspect, the present invention provide the selenium-rich fungus cultivation method using above-mentioned culture medium for cultivating comprising:
Method one, agaric extraction slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out dry-mixed equal
It is even, then turning mixes repeatedly with sawdust, mixes thoroughly after adding water, moisture content is 55~60%, sieving, bored 1~5h of heap；Using routine
Method makes bacterium bag, is sealed with bacteria stick, damp and hot 121~123 DEG C of sterilizings, the 8~10h of 1~2h or normal-pressure sterilization of high pressure, after cooling,
It is inoculated with agaric fungus, is cultivated in 23~27 DEG C, routine observation hypha growth condition；Bacterium bag is planted after covering with referring to the conventional full light of black fungus
Training mode or greenhouse hanging bag mode carry out ear management, harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
Further, in preferred embodiments of the present invention, it is 3000g, sawdust 80000g, rice chaff that agaric, which extracts slag,
8000g, wheat bran skin 3000g, bean powder 3000g, lime 500g, gypsum 500g and broken wall Se-enriched yeast are 2000g.Agaric is extracted
Slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, the progress of broken wall Se-enriched yeast are dry-mixed uniformly, then turning mixes repeatedly with sawdust,
It mixes thoroughly, moisture content 57%, is sieved after adding water, bored heap 3h；Bacterium bag is made using conventional method, about 2.5 jin of every bag of weight in wet base, is used
Bacteria stick sealing, normal-pressure sterilization 10h after cooling, are inoculated with agaric fungus, cultivate in 25 DEG C, routine observation hypha growth condition；Bacterium bag is long
Ear management, harvesting and sunning are carried out referring to the full light cultivation mode of conventional black fungus or greenhouse hanging bag mode after full；Statistics is real
It tests as a result, calculating single bag of yield.
Method two, agaric extraction slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out dry-mixed equal
It is even, then turning mixes repeatedly with sawdust, mixes thoroughly after adding water, moisture content is 55~60%, sieving, bored 1~5h of heap；It will be equipped with training
The square plate for supporting base is neatly arranged on vehicle of sterilization, be pushed between sterilizing using damp and hot 121~123 DEG C of 1~2h of sterilizing of high pressure or
Person 8~10h of normal-pressure sterilization shifts vehicle of sterilization onto the preparation between solid fermentation, cools to 26~28 DEG C, inoculation produces lignin
Degrading enzyme fungi and/or 0.1~0.3 part of cellulase-producing fungi liquid seeds, the interior holding temperature of solid fermentation are 26~28
DEG C, it is 70%~80% that 1~3d of fermentation, which keeps the humidity in sweathouse,；From 4d, ambient humidity 30%~50%, hair are kept
5~10d of ferment；Bacterium bag is made using conventional method, is sealed with bacteria stick, damp and hot 121~123 DEG C of 1~2h of sterilizing of high pressure or normal pressure
Sterilize 8~10h, after cooling, is inoculated with agaric fungus, cultivates in 23~27 DEG C, routine observation hypha growth condition；Bacterium bag is joined after covering with
The full light cultivation mode of more solito black fungus or greenhouse hanging bag mode carry out ear management, harvesting and sunning；Statistical experiment as a result,
Calculate single bag of yield.
Further, in preferred embodiments of the present invention, it is 3000g, sawdust 80000g, rice chaff that agaric, which extracts slag,
8000g, wheat bran skin 3000g, bean powder 3000g, lime 500g, gypsum 500g and broken wall Se-enriched yeast are 2000g.Agaric is extracted
Slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, the progress of broken wall Se-enriched yeast are dry-mixed uniformly, then turning mixes repeatedly with sawdust,
It mixes thoroughly, moisture content 58%, is sieved after adding water, bored heap 3h；Square plate equipped with culture medium is neatly arranged on vehicle of sterilization,
It is pushed between sterilizing using normal-pressure sterilization 10h, vehicle of sterilization is shifted onto the preparation between solid fermentation, cool to 27 DEG C, inoculation
It produces lignin-degrading enzymes fungi and cellulase-producing fungi liquid seeds is respectively 200g, the interior holding temperature of solid fermentation is 27
DEG C, it is 75% that 3d, which keeps the ambient humidity in sweathouse, before fermenting；From 4d, ambient humidity 40% is kept, ferment 7d；Using
Conventional method makes bacterium bag, about 2.5 jin of every bag of weight in wet base, is sealed with bacteria stick, normal-pressure sterilization 10h, after cooling, is inoculated with agaric fungus, in
25 DEG C of cultures, routine observation hypha growth condition；Bacterium bag is hung after covering with referring to the full light cultivation mode of conventional black fungus or greenhouse
Bag mode carries out ear management, harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
Black fungus parent species used in the present invention produce lignin-degrading enzymes fungi and cellulase-producing fungi is made according to a conventional method
Standby, Se content is that the broken wall Se-enriched yeast of 8000mg/kg is bought on the market with other nutrient media components.
Compared with prior art, the beneficial effect comprise that
The equal fructification dry weight of the bag of agaric and selenium-rich amount compared with prior art, are significantly increased.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
Feature and performance of the invention are described in further detail with reference to embodiments:
Embodiment 1
Agaric extract slag be 5000g, sawdust 75000g, rice chaff 8000g, wheat bran skin 6000g, bean powder 3000g, lime
200g, gypsum 800g and broken wall Se-enriched yeast are 1000g.
Agaric extract slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with wood
Turning mixes bits repeatedly, mixes thoroughly, moisture content 55%, is sieved after adding water, bored heap 5h；Bacterium bag is made using conventional method, often
About 2.5 jin of weight in wet base of bag, is sealed with bacteria stick, after the damp and hot 121 DEG C of sterilizing 2h of high pressure are cooling, is inoculated with agaric fungus, is cultivated in 23 DEG C, periodically
Observe hypha growth condition；The full light cultivation mode of the conventional black fungus of reference or greenhouse hanging bag mode carry out produce agaric after bacterium bag covers with
Management, harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
Embodiment 2
Agaric extract slag be 3000g, sawdust 80000g, rice chaff 5000g, wheat bran skin 6000g, bean powder 3000g, lime
800g, gypsum 200g and broken wall Se-enriched yeast are 3000g.
Agaric extract slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with wood
Turning mixes bits repeatedly, mixes thoroughly, moisture content 60%, is sieved after adding water, bored heap 1h；Bacterium bag is made using conventional method, often
About 2.5 jin of weight in wet base of bag, is sealed with bacteria stick, after the damp and hot 123 DEG C of sterilizing 1h of high pressure are cooling, is inoculated with agaric fungus, is cultivated in 27 DEG C, periodically
Observe hypha growth condition；The full light cultivation mode of the conventional black fungus of reference or greenhouse hanging bag mode carry out produce agaric after bacterium bag covers with
Management, harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
Embodiment 3
Agaric extract slag be 1000g, sawdust 80000g, rice chaff 8000g, wheat bran skin 6000g, bean powder 2000g, lime
500g, gypsum 500g and broken wall Se-enriched yeast are 2000g.
Agaric extract slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with wood
Turning mixes bits repeatedly, mixes thoroughly, moisture content 57%, is sieved after adding water, bored heap 3h；Bacterium bag is made using conventional method, often
About 2.5 jin of weight in wet base of bag, is sealed, normal-pressure sterilization 8h with bacteria stick, after cooling, is inoculated with agaric fungus, is cultivated in 25 DEG C, routine observation mycelia
Growing state；The full light cultivation mode of the conventional black fungus of reference or greenhouse hanging bag mode carry out ear management, adopt after bacterium bag covers with
It receives and dries；Statistical experiment is as a result, calculate single bag of yield.
Embodiment 4
Agaric extract slag be 3000g, sawdust 80000g, rice chaff 8000g, wheat bran skin 3000g, bean powder 3000g, lime
500g, gypsum 500g and broken wall Se-enriched yeast are 2000g.
Agaric extract slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with wood
Turning mixes bits repeatedly, mixes thoroughly, moisture content 57%, is sieved after adding water, bored heap 3h；Bacterium bag is made using conventional method, often
About 2.5 jin of weight in wet base of bag, is sealed, normal-pressure sterilization 10h with bacteria stick, after cooling, is inoculated with agaric fungus, is cultivated in 25 DEG C, routine observation bacterium
Silk growing state；Bacterium bag cover with after referring to the full light cultivation mode of conventional black fungus or greenhouse hanging bag mode carry out ear management,
Harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
Embodiment 5
Agaric extract slag be 5000g, sawdust 75000g, rice chaff 8000g, wheat bran skin 6000g, bean powder 3000g, lime
200g, gypsum 800g and broken wall Se-enriched yeast are 1000g.
Agaric extract slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with wood
Turning mixes bits repeatedly, mixes thoroughly, moisture content 55%, is sieved after adding water, bored heap 5h；Fitly by the square plate equipped with culture medium
It is placed on vehicle of sterilization, is pushed between sterilizing using the damp and hot 121 DEG C of sterilizings 2h of high pressure, vehicle of sterilization is shifted onto the standard between solid fermentation
It is standby interior, 26 DEG C are cooled to, inoculation produces lignin-degrading enzymes fungi 300g and cellulase-producing fungi liquid seeds 100g, solid
It is 28 DEG C that temperature is kept in sweathouse, and it is 70% that 3d, which keeps the ambient humidity in sweathouse, before fermenting；From 4d, environment is kept
Humidity 50%, ferment 5d；Bacterium bag is made using conventional method, about 2.5 jin of every bag of weight in wet base, is sealed with bacteria stick, damp and hot 121 DEG C of high pressure
Sterilize 2h, after cooling, is inoculated with agaric fungus, cultivates in 23 DEG C, routine observation hypha growth condition；Bacterium bag is black referring to routine after covering with
The full light cultivation mode of agaric or greenhouse hanging bag mode carry out ear management, harvesting and sunning；Statistical experiment is as a result, calculate single bag
Yield.
Embodiment 6
Agaric extract slag be 3000g, sawdust 80000g, rice chaff 5000g, wheat bran skin 6000g, bean powder 3000g, lime
800g, gypsum 200g and broken wall Se-enriched yeast are 3000g.
Agaric extract slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with wood
Turning mixes bits repeatedly, mixes thoroughly, moisture content 60%, is sieved after adding water, bored heap 1h；Fitly by the square plate equipped with culture medium
It is placed on vehicle of sterilization, is pushed between sterilizing using the damp and hot 123 DEG C of sterilizings 1h of high pressure, vehicle of sterilization is shifted onto the standard between solid fermentation
It is standby interior, 28 DEG C are cooled to, inoculation produces lignin-degrading enzymes fungi 100g and cellulase-producing fungi liquid strain 300g, solid hair
The interior holding temperature of ferment is 26 DEG C, and it is 80% that 3d, which keeps the ambient humidity in sweathouse, before fermenting；From 4d, environmental wet is kept
Degree 30%, ferment 10d；Bacterium bag is made using conventional method, about 2.5 jin of every bag of weight in wet base, is sealed with bacteria stick, damp and hot 121 DEG C of high pressure
Sterilize 2h, after cooling, is inoculated with agaric fungus, cultivates in 27 DEG C, routine observation hypha growth condition；Bacterium bag is black referring to routine after covering with
The full light cultivation mode of agaric or greenhouse hanging bag mode carry out ear management, harvesting and sunning；Statistical experiment is as a result, calculate single bag
Yield.
Embodiment 7
Agaric extract slag be 1000g, sawdust 80000g, rice chaff 8000g, wheat bran skin 6000g, bean powder 2000g, lime
500g, gypsum 500g and broken wall Se-enriched yeast are 2000g.
Agaric extract slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with wood
Turning mixes bits repeatedly, mixes thoroughly, moisture content 58%, is sieved after adding water, bored heap 3h；Fitly by the square plate equipped with culture medium
It is placed on vehicle of sterilization, is pushed between sterilizing using normal-pressure sterilization 8h, vehicle of sterilization is shifted onto the preparation between solid fermentation, drop
For temperature to 27 DEG C, it is respectively 200g that inoculation, which produces lignin-degrading enzymes fungi and cellulase-producing fungi liquid seeds, between solid fermentation
Interior holding temperature is 27 DEG C, and it is 75% that 3d, which keeps the ambient humidity in sweathouse, before fermenting；From 4d, ambient humidity is kept
40%, ferment 7d；Bacterium bag is made using conventional method, about 2.5 jin of every bag of weight in wet base, is sealed with bacteria stick, normal-pressure sterilization 9h, it is cooling
Afterwards, it is inoculated with agaric fungus, is cultivated in 25 DEG C, routine observation hypha growth condition；Bacterium bag is planted after covering with referring to the conventional full light of black fungus
Training mode or greenhouse hanging bag mode carry out ear management, harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
Embodiment 8
Agaric extract slag be 3000g, sawdust 80000g, rice chaff 8000g, wheat bran skin 3000g, bean powder 3000g, lime
500g, gypsum 500g and broken wall Se-enriched yeast are 2000g.
Agaric extract slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with wood
Turning mixes bits repeatedly, mixes thoroughly, moisture content 58%, is sieved after adding water, bored heap 3h；Fitly by the square plate equipped with culture medium
It is placed on vehicle of sterilization, is pushed between sterilizing using normal-pressure sterilization 10h, vehicle of sterilization is shifted onto the preparation between solid fermentation,
27 DEG C are cooled to, inoculation produces lignin-degrading enzymes fungi and cellulase-producing fungi liquid seeds are respectively 200g, solid fermentation
Interior holding temperature is 27 DEG C, and it is 75% that 3d, which keeps the ambient humidity in sweathouse, before fermenting；From 4d, ambient humidity is kept
40%, ferment 7d；Bacterium bag is made using conventional method, about 2.5 jin of every bag of weight in wet base, is sealed with bacteria stick, normal-pressure sterilization 10h, it is cooling
Afterwards, it is inoculated with agaric fungus, is cultivated in 25 DEG C, routine observation hypha growth condition；Bacterium bag is planted after covering with referring to the conventional full light of black fungus
Training mode or greenhouse hanging bag mode carry out ear management, harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
Comparative example 1
Sawdust 80000g, rice chaff 8000g, wheat bran skin 3000g, bean powder 3000g, lime 500g, gypsum 500g and broken wall
Se-enriched yeast is 2000g.
Rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with sawdust turning repeatedly
It mixes, is mixed thoroughly after adding water, moisture content 57%, is sieved, bored heap 3h；Bacterium bag, every bag of weight in wet base about 2.5 are made using conventional method
Jin, is sealed, normal-pressure sterilization 10h with bacteria stick, after cooling, is inoculated with agaric fungus, is cultivated in 25 DEG C, routine observation hypha growth condition；
The full light cultivation mode of the conventional black fungus of reference or greenhouse hanging bag mode carry out ear management, harvesting and sunning after bacterium bag covers with；
Statistical experiment is as a result, calculate single bag of yield.
Comparative example 2
Sawdust 80000g, rice chaff 8000g, wheat bran skin 3000g, bean powder 3000g, lime 500g, gypsum 500g and broken wall
Se-enriched yeast is 2000g.
Rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast carry out it is dry-mixed uniformly, then with sawdust turning repeatedly
It mixes, is mixed thoroughly after adding water, moisture content 58%, is sieved, bored heap 3h；Square plate equipped with culture medium is neatly arranged and is being sterilized
Che Shang is pushed between sterilizing using normal-pressure sterilization 10h, vehicle of sterilization is shifted onto the preparation between solid fermentation, cool to 27 DEG C,
Inoculation produces lignin-degrading enzymes fungi and cellulase-producing fungi liquid seeds are respectively 200g, the interior holding temperature of solid fermentation
It is 27 DEG C, it is 75% that 3d, which keeps the ambient humidity in sweathouse, before fermenting；From 4d, ambient humidity 40% is kept, ferment 7d；
Bacterium bag is made using conventional method, about 2.5 jin of every bag of weight in wet base, is sealed with bacteria stick, normal-pressure sterilization 10h, after cooling, is inoculated with agaric
Bacterium is cultivated, routine observation hypha growth condition in 25 DEG C；The full light cultivation mode or big of the conventional black fungus of reference after bacterium bag covers with
Canopy hanging bag mode carries out ear management, harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
Beneficial effects of the present invention are as follows:
As can be seen from the above table, the present invention contains the Examples 1 to 4 list sack entity dry weight and fructification that agaric extracts slag
The prior art comparative example 1 that selenium-rich amount extracts slag compared with no agaric is significantly improved；The present invention contains agaric and extracts slag and through solid
The single sack entity dry weight of the embodiment 5~8 of body fermentation and fructification selenium-rich amount extract slag compared with no agaric and through solid fermentation
Prior art comparative example 2 and slag is extracted without agaric but prior art comparative example 1 without solid fermentation is significantly increased；The present invention
The single sack entity dry weight of embodiment 5~8 and fructification selenium-rich amount containing agaric extraction slag and through solid fermentation is more of the invention
Examples 1 to 4 containing agaric extraction slag but without solid fermentation is significantly increased.
Unspecified link is the common knowledge that those of ordinary skill in the art can voluntarily select in the present invention.Though
So, above the present invention is described in detail with a general description of the specific embodiments, but on basis of the invention
On, it can be made some modifications or improvements, this is obvious for those of ordinary skill in the art.Therefore, not
These modifications or improvements on the basis of deviation spirit of that invention, fall within the scope of the claimed invention.
Claims (10)
1. a kind of culture medium for cultivating of selenium-rich agaric, which is characterized in that the culture medium for cultivating include agaric extract slag, sawdust,
Rice chaff, wheat bran skin, bean powder, lime, gypsum, broken wall Se-enriched yeast.
2. culture medium for cultivating according to claim 1, which is characterized in that according to parts by weight, it is 1 that the agaric, which extracts slag,
~5 parts, 75~80 parts of the sawdust, 5~8 parts of the rice chaff, 3~6 parts of the wheat bran skin, the bean powder be 2~3 parts, it is described
0.2~0.8 part of lime, 0.2~0.8 part of the gypsum and the broken wall Se-enriched yeast are 1~3 part.
3. culture medium for cultivating according to claim 2, which is characterized in that further include producing lignin drop according to parts by weight
Solve 0.1~0.3 part of enzyme fungi and/or 0.1~0.3 part of cellulase-producing fungi liquid seeds.
4. a kind of cultural method of selenium-rich agaric, which is characterized in that extract agaric in culture medium for cultivating as claimed in claim 2
Slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, the progress of broken wall Se-enriched yeast are dry-mixed uniformly, then turning mixes repeatedly with sawdust,
It mixes thoroughly, is sieved after adding water, bored heap；Bacterium bag is made using conventional method, about 2.5 jin of every bag of weight in wet base, is sealed with bacteria stick, high pressure is damp and hot
121~123 DEG C of sterilizing 8~10h of 1~2h or normal-pressure sterilization after cooling, are inoculated with agaric fungus, cultivate in 23~27 DEG C, periodically see
Examine hypha growth condition；The full light cultivation mode of the conventional black fungus of reference or greenhouse hanging bag mode carry out out syrinx after bacterium bag covers with
Reason, harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
5. a kind of cultural method of selenium-rich agaric, which is characterized in that extract agaric in culture medium for cultivating as claimed in claim 3
Slag, rice chaff, wheat bran skin, bean powder, lime, gypsum, the progress of broken wall Se-enriched yeast are dry-mixed uniformly, then turning mixes repeatedly with sawdust,
It mixes thoroughly, is sieved after adding water, bored heap；Square plate equipped with culture medium is neatly arranged on vehicle of sterilization, is pushed between sterilizing and uses
Damp and hot 121~123 DEG C of sterilizings, the 8~10h of 1~2h or normal-pressure sterilization of high pressure, vehicle of sterilization is shifted between the preparation between solid fermentation
It is interior, 26~28 DEG C are cooled to, inoculation produces lignin-degrading enzymes fungi and/or cellulase-producing fungi liquid seeds 0.1~0.3
Part, the interior holding temperature of solid fermentation is 26~28 DEG C, and ferment 5~10d；Bacterium bag is made using conventional method, every bag of weight in wet base is about
It 2.5 jin, is sealed with bacteria stick, damp and hot 121~123 DEG C of sterilizings, the 8~10h of 1~2h or normal-pressure sterilization of high pressure, after cooling, inoculation wood
Ear bacterium is cultivated, routine observation hypha growth condition in 23~27 DEG C；The full light cultivation mode of the conventional black fungus of reference after bacterium bag covers with
Or greenhouse hanging bag mode carries out ear management, harvesting and sunning；Statistical experiment is as a result, calculate single bag of yield.
6. cultural method according to claim 4 or 5, which is characterized in that culture medium moisture content is 55 after described plus water
~60%.
7. cultural method according to claim 4 or 5, which is characterized in that the 1~5h of bored heap time.
8. cultural method according to claim 4 or 5, which is characterized in that cultivated after the inoculation agaric fungus in 25 DEG C.
9. cultural method according to claim 5, which is characterized in that 3d keeps the environmental wet in sweathouse before the fermentation
Degree is 70%~80%；From 4d, ambient humidity 30%~50% is kept.
10. cultural method according to claim 5, which is characterized in that the fermentation time is 7d.
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