CN110438092A - 病毒运送培养基及其制备方法和应用 - Google Patents
病毒运送培养基及其制备方法和应用 Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
组分名称 | 贮存液浓度 | 用量 | 终浓度 |
Hank’s平衡盐溶液 | 4× | 125mL | 0.5× |
明胶 | 5% | 20mL | 1g/L |
BSA | 10% | 25mL | 2.5g/L |
蔗糖 | 5% | 20mL | 1g/L |
L-半胱氨酸 | 0.015mmol/mL | 6.6mL | 0.1mM/L |
L-谷氨酸 | 0.015mmol/mL | 6.6mL | 0.1mM/L |
Hepes | 1M/L | 5mL | 5mM/L |
两性霉素B | 2.5mg/mL | 4mL | 10mg/L |
多粘菌素B | 2mg/mL | 5mL | 10mg/L |
庆大霉素 | 10mg/mL | 1mL | 10mg/L |
水 | / | 定容至1L | / |
组分名称 | 贮存液浓度 | 用量(配制1L) | 终浓度 |
Hank’s平衡盐溶液 | 4× | 250mL | 1× |
明胶 | 5% | 50mL | 2.5g/L |
BSA | 10% | 50mL | 5g/L |
蔗糖 | 5% | 50mL | 2.5g/L |
L-半胱氨酸 | 0.015mmol/mL | 10mL | 0.150mM/L |
L-谷氨酸 | 0.015mmol/mL | 10mL | 0.150mM/L |
Hepes | 1M | 10mL | 10mM/L |
两性霉素B | 2.5mg/mL | 8mL | 20mg/L |
多粘菌素B | 2mg/mL | 10mL | 20mg/L |
庆大霉素 | 10mg/mL | 2mL | 20mg/L |
水 | / | 定容至1L | / |
组分名称 | 贮存液浓度 | 用量(配制1L) | 终浓度 |
Hank’s平衡盐溶液 | 4× | 500mL | 2× |
明胶 | 5% | 100mL | 5g/L |
BSA | 10% | 100mL | 10g/L |
蔗糖 | 5% | 100mL | 5g/L |
L-半胱氨酸 | 0.015mmol/mL | 20mL | 0.3mM/L |
L-谷氨酸 | 0.015mmol/mL | 20mL | 0.3mM/L |
Hepes | 1M | 20mL | 20mM/L |
两性霉素B | 2.5mg/mL | 16mL | 40mg/L |
多粘菌素B | 2mg/mL | 20mL | 40mg/L |
庆大霉素 | 10mg/mL | 4mL | 40mg/L |
水 | / | 定容至1L | / |
Claims (10)
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110982876A (zh) * | 2020-03-04 | 2020-04-10 | 圣湘生物科技股份有限公司 | 病毒核酸检测的预处理方法、预处理液、试剂盒及其用途 |
CN111876330A (zh) * | 2020-07-07 | 2020-11-03 | 广州金域医学检验中心有限公司 | 一种适用于核酸检测的病毒运送培养基及其制备方法 |
CN112314593A (zh) * | 2020-11-03 | 2021-02-05 | 武汉尚飞生物科技有限公司 | 一种适用于病毒样本常温保存的保存液及其制备方法 |
CN112779164A (zh) * | 2021-01-28 | 2021-05-11 | 苏州赛普生物科技有限公司 | 一种病毒运送培养基 |
CN113462815A (zh) * | 2021-06-17 | 2021-10-01 | 深圳逗点生物技术有限公司 | 病毒培养基及其制备方法、病毒转运培养瓶和病毒采样管 |
WO2022101877A1 (pt) * | 2020-11-13 | 2022-05-19 | Targetalent, Lda | Composição de criopreservação de amostras biológicas para pcr |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110982876A (zh) * | 2020-03-04 | 2020-04-10 | 圣湘生物科技股份有限公司 | 病毒核酸检测的预处理方法、预处理液、试剂盒及其用途 |
CN111876330A (zh) * | 2020-07-07 | 2020-11-03 | 广州金域医学检验中心有限公司 | 一种适用于核酸检测的病毒运送培养基及其制备方法 |
CN111876330B (zh) * | 2020-07-07 | 2022-08-30 | 广州金域医学检验中心有限公司 | 一种适用于核酸检测的病毒运送培养基及其制备方法 |
CN112314593A (zh) * | 2020-11-03 | 2021-02-05 | 武汉尚飞生物科技有限公司 | 一种适用于病毒样本常温保存的保存液及其制备方法 |
WO2022101877A1 (pt) * | 2020-11-13 | 2022-05-19 | Targetalent, Lda | Composição de criopreservação de amostras biológicas para pcr |
CN112779164A (zh) * | 2021-01-28 | 2021-05-11 | 苏州赛普生物科技有限公司 | 一种病毒运送培养基 |
CN113462815A (zh) * | 2021-06-17 | 2021-10-01 | 深圳逗点生物技术有限公司 | 病毒培养基及其制备方法、病毒转运培养瓶和病毒采样管 |
CN113462815B (zh) * | 2021-06-17 | 2023-11-24 | 深圳逗点生物技术有限公司 | 病毒培养基及其制备方法、病毒转运培养瓶和病毒采样管 |
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