具体实施方式
以下所述是本发明实施例的优选实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本发明实施例原理的前提下,还可以做出若干改进和润饰,这些改进和润饰也视为本发明实施例的保护范围。
若无特别说明,本发明实施例所采用的原料及其它化学试剂皆为市售商品。
1、α-氨基酸酯酰基转移酶的表达
(1)α-氨基酸酯酰基转移酶的核苷酸序列
通过实验筛选获得α-氨基酸酯酰基转移酶XPD-01,所述α-氨基酸酯酰基转移酶XPD01的核苷酸序列如SEQ ID NO:37所示的核苷酸序列;
(2)根据表1设计的上、下游引物,通过反向PCR技术对α-氨基酸酯酰基转移酶XPD-01进行突变等基因改造,制备得到α-氨基酸酯酰基转移酶XPD-02至XPD-07;其中,所述α-氨基酸酯酰基转移酶XPD-02至XPD-07对应的核苷酸及氨基酸序列参见表2:
表1.突变引物序列
所述PCR扩增体系实验参数如下:
PCR扩增程序为:98℃预变性2min;98℃变性10s;55-65℃退火30s;72℃延伸7min;30个循环后,72℃延伸10min。PCR产物经胶回收试剂盒纯化后,分别利用限制性内切酶进行酶切,酶切后用T4连接酶连接至质粒pET28a(+)。提取质粒,经过测序成功后即得到的重组质粒。例如,以α-氨基酸酯酰基转移酶XPD-02为例,提供上游引物和下游引物,并通过实验得到的α-氨基酸酯酰基转移酶(XPD-02)的基因编码序列;所述α-氨基酸酯酰基转移酶XPD-02的基因编码序列包括如SEQ ID NO:1所示的核苷酸序列;
将所述XPD-02的基因编码序列插入到pET28a(+)载体质粒的BamH I和Hind III酶切位点之间。所述XPD-02的基因编码序列插入到pET28a(+)载体质粒时,所述XPD的基因编码序列的5’端添加起始密码子(如ATG)与pET28a(+)载体质粒中BamH I酶切位点相连,3’端还添加有终止密码子(如TAA)与pET28a(+)载体质粒中Hind III酶切位点相连。然后转入大肠杆菌感受态细胞DH5α,进行阳性克隆PCR鉴定和测序鉴定,以成功构建pET28a-XPD-02重组质粒,如图1所示的重组质粒pET28a-XPD-02的质粒图谱。其他α-氨基酸酯酰基转移酶XPD-01,及XPD-03至XPD-07的重组质粒可同样参照上述方法制得。
选取表达宿主细胞,所述表达宿主细胞包括大肠杆菌和酵母菌中的一种或多种。将制备得到的重组质粒转至表达宿主细胞-大肠杆菌中,表达得到对应的α-氨基酸酯酰基转移酶XPD-01至XPD-07。
表2.α-氨基酸酯酰基转移酶数据表
2、α-氨基酸酯酰基转移酶酶活力测定
(1)取0.5mL菌液,加入500μL含有200mM谷氨酰胺,200mM L-丙氨酸甲酯盐酸盐,在pH=9的0.1M硼酸-氢氧化钠缓冲液,混匀后置25℃恒温水浴锅中反应5min,加入等体积的1.7%磷酸(v/v)溶液终止反应,12000r/min离心5min,取反应液上清按高效液相色谱(HPLC)方法测定丙谷二肽浓度,(备注:一个酶活单位定义为1min内生成1μmol产物所需酶量);实验测试参数参见下表(备注:按体积比计算):
色谱柱 |
月旭UItimate XB-NH2 5μm×250×4.6mm |
流动相 |
0.05mol/L磷酸二氢钾缓冲液(用磷酸调节pH值至4.0)-乙腈(35∶65) |
仪器型号 |
Agilent1260 |
检测器 |
UV215nm |
流速 |
1.0mL/min |
柱温 |
30℃ |
进样量 |
20μL |
样品处理 |
用流动相稀释 |
产品浓度 |
0.5mg/mL左右 |
标准曲线的绘制:设置进样量和进样度分别为2μL、4μL、5μL、6μL、8μL,绘制标准曲线;计算得到的α-氨基酸酯酰基转移酶XPD-01至XPD-07的酶活力相关数据,并计算通过基因改造获得的α-氨基酸酯酰基转移酶XPD-02至XPD-07相比于α-氨基酸酯酰基转移酶XPD-01的酶活力增长率,如下表3所示,:
表3.α-氨基酸酯酰基转移酶XPD-01至XPD-07的酶活力数据表
实验测试结果显示,本申请所述的α-氨基酸酯酰基转移酶XPD-02至XPD-07具有突出的酶活力,相较于α-氨基酸酯酰基转移酶XPD-01均具有较高的酶活力增长率,且pH稳定性也有较大的范围增长,特别是α-氨基酸酯酰基转移酶XPD-04的酶活力增长率高于50%。
3、α-氨基酸酯酰基转移酶在重组改造后的表达宿主细胞上的表达
(1)选取表达宿主细胞,所述表达宿主细胞包括大肠杆菌和酵母菌中的一种或多种。所述大肠杆菌可以为大肠杆菌JM109(DE3)或大肠杆菌BL21(DE3)。利用CRISPR/Cas9基因编辑技术对所述表达宿主细胞进行重组,包括如下步骤:
a)基因克隆
设计引物,分别以PASGF/PASGR、PBSGF/PBSGR、PDSGF/PDSGR和PNSGF/PNSGR的引物对,以质粒pTargetF为模板,PCR扩增出可识别pepA、pepB、pepD和pepN基因的pTargetF,并带有与基因组中目的片段20bp碱基互补的sgRNA,然后分别命名为sgRNA-pepA、sgRNA-pepB、sgRNA-pepD和sgRNA-pepN;然后将所述扩增的PCR产物转化至大肠杆菌DH5α内,分别利用上述引物鉴定阳性克隆并测序,将测序正确的阳性克隆分别命名为pTargetF-pepA、pTargetF-pepB、pTargetF-pepD和pTargetF-pepN。
分别以引物PA01/PA02和PA03/PA04、PB01/PB02和PB03/PB04、PD01/PD02和PD03/PD04、PN01/PN02和PN03/PN04扩增用于同源重组的pepA、pepB、pepD和pepN基因的上下游同源臂片段pepA上/下、pepB上/下、pepD上/下和pepN上/下,然后通过重叠PCR(Overlap PCR)将pepA上/下、pepB上/下、pepD上/下和pepN上/下分别连接起来,将测序条带正确的分别命名pepA同、pepB同、pepD同和pepN同。
其中,所述PA01包括如SEQ ID NO:13所示的核苷酸序列;所述PA02包括如SEQ IDNO:14所示的核苷酸序列;所述PA03包括如SEQ ID NO:15所示的核苷酸序列;所述PA04包括如SEQ ID NO:16所示的核苷酸序列;所述PASGF包括如SEQ ID NO:17所示的核苷酸序列;所述PASGR包括如SEQ ID NO:18所示的核苷酸序列。所述PB01包括如SEQ ID NO:19所示的核苷酸序列;所述PB02包括如SEQ ID NO:20所示的核苷酸序列;所述PB03包括如SEQ ID NO:21所示的核苷酸序列;所述PB04包括如SEQ ID NO:22所示的核苷酸序列;所述PBSGF包括如SEQ ID NO:23所示的核苷酸序列;所述PBSGR包括如SEQ ID NO:24所示的核苷酸序列。所述PD01包括如SEQ ID NO:25所示的核苷酸序列;所述PD02包括如SEQ ID NO:26所示的核苷酸序列;所述PD03包括如SEQ ID NO:27所示的核苷酸序列;所述PD04包括如SEQ ID NO:28所示的核苷酸序列;所述PDSGF包括如SEQ ID NO:29所示的核苷酸序列;所述PDSGR包括如SEQID NO:30所示的核苷酸序列。所述PN01包括如SEQ ID NO:31所示的核苷酸序列;所述PN02包括如SEQ ID NO:32所示的核苷酸序列;所述PN03包括如SEQ ID NO:33所示的核苷酸序列;所述PN04包括如SEQ ID NO:34所示的核苷酸序列;所述PNSGF包括如SEQ ID NO:35所示的核苷酸序列;所述PNSGR包括如SEQ ID NO:36所示的核苷酸序列。
b)基因敲除
将pCas转化到表达宿主细胞中,然后挑取含有pCas的单克隆,再加入50mg/L卡那霉素的LB培养基中,30℃、200rpm培养至OD600为0.2时,向摇瓶中加入终浓度为10mM的阿拉伯糖诱导pCas载体上λ-Red蛋白的表达,然后摇至OD600为0.6时回收表达宿主细胞并制备电转感受态。电转时,向100μL的制备得到的感受态细胞中加入100ng的pTargetF质粒(包括pTargetF-pepA、pTargetF-pepB、pTargetF-pepD和pTargetF-pepN中的一种或多种)和400ng的同源臂DNA片段(包括pepA同、pepB同、pepD同和pepN同中的一种或多种),轻柔混合后,加入到预冷的0.2cm电转杯中,在2.2kv的条件下放入电转仪(Bio-Rad)中电转,电转完后迅速加入1mL的LB培养基,30℃、180rpm培养1h复苏,涂含有50mg/L卡那霉素和50mg/L壮观霉素的LB双抗平板上,30℃培养;挑取单克隆,PCR验证正确后消除pTargetF和pCas。
所述消除pTargetF和pCas的步骤包括:将重组的表达宿主细胞(含有pTargetF和pCas)培养在50mg/L卡那霉素LB培养基中,并加入终浓度为0.5mM IPTG诱导培养过夜,并将其涂布于含50mg/L卡那霉素的平板上用于消除pTargetF,待平板上长出单克隆,挑取该单克隆在含50mg/L壮观霉素的液体LB中培养过夜,不长即为pTargetF消除的重组的表达宿主细胞,然后挑取该重组的表达宿主细胞在42℃的环境中培养过夜,用以消除pCas。
(2)α-氨基酸酯酰基转移酶的表达
以α-氨基酸酯酰基转移酶XPD-02为例,将构建的重组质粒pET28a-XPD-02转入重组的表达宿主细胞中,并以1%的接种量接种至含有4mL的LB培养基中,维持恒定的37℃,200rpm的摇晃速率,过夜培养后,将表达宿主细胞以1%的接种量转接到含有1LLB培养基(50μg/mL卡那霉素)的三角瓶中,继续37℃恒温培养至培养基中的OD600值达到0.6左右,加入终浓度为度0.5mM的诱导剂IPTG,在30℃条件培养16-20小时后离心收集表达宿主细胞。将表达宿主细胞用100mM硼砂-硼酸缓冲液(pH=8.0)进行重悬并保存得到表达宿主细胞溶液。通过参数调节,可以制得质量分数各异的表达宿主细胞溶液;实验过程中,通常加入质量分数为5%-50%的表达宿主细胞溶液。
取部分表达宿主细胞溶液,经超声破碎和离心,收集所述XPD-02;采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)鉴定。本实施方式表达获得的XPD和XPD的分子大小均与相应蛋白的理论计算值相近,其中,XPD-02的理论分子量为66.8kDa。此外,对收集得到的XPD-02进行进一步纯化,可制得XPD-02酶粉。其他α-氨基酸酯酰基转移酶XPD-03至XPD-07可采用上述制备方法相应制备得到,本实施方式不做过多论述。
实施例1
一种丙谷二肽的制备方法,包括:
将180g甲醇加入1L反应釜中,并降温至15℃;将78.6g二氯亚砜在10℃滴加入反应釜中;二氯亚砜滴加完全后将L-丙氨酸加入到反应釜中,保温搅拌30min,再缓慢升温至25℃,搅拌1h,再升温至50℃搅拌2h;50℃减压蒸馏,结晶制得82g L-丙氨酸甲酯盐酸盐。
取反应底物L-丙氨酸甲酯盐酸盐20g和谷氨酰胺21g溶解在纯水(400mL)中,将100mL XPD-02细胞溶液缓慢加入反应底物中,其中XPD-02细胞在整个反应液中的质量百分比为1.5%,实时监控反应液的pH值,用5M氢氧化钠溶液调节pH值在8,温度保持在25℃。反应过程中取样稀释后用液相色谱法检测生成丙谷二肽量。丙谷二肽开始分解时停止反应。反应液离心后,上清加入终浓度20%甲醇60℃水浴30min,0.5%活性炭搅拌30min,离心得上清反应液625mL,得到丙谷二肽含量59.9g/L,转化率92.0%。
对制得的丙谷二肽进行取样检测,得到丙谷二肽核磁氢谱(图2)、和丙谷二肽核磁碳谱(图3)。
实施例2
一种丙谷二肽的制备方法,包括:
将180g甲醇加入1L反应釜中,并降温至10℃;将78.6g二氯亚砜在0℃滴加入反应釜中;二氯亚砜滴加完全后将L-丙氨酸加入到反应釜中,保温搅拌60min,再缓慢升温至30℃,搅拌2h,再升温至45℃搅拌2h;50℃减压蒸馏,结晶制得82.7g L-丙氨酸甲酯盐酸盐。
取反应底物L-丙氨酸甲酯盐酸盐20g和谷氨酰胺21g溶解在纯水(400mL)中,将100mL XPD-02细胞溶液缓慢加入反应底物中,其中XPD-02细胞在整个反应液中的质量百分比为5.0%,实时监控反应液的pH值,用5M氢氧化钠溶液调节pH值在9,温度保持在40℃。反应过程中取样稀释后用液相色谱法检测生成丙谷二肽量。丙谷二肽开始分解时停止反应。反应液离心后,上清加入终浓度20%甲醇60℃水浴30min,0.5%活性炭搅拌30min,离心得上清反应液625mL,得到丙谷二肽含量60.6g/L,转化率93.0%。
实施例3
一种丙谷二肽的制备方法,包括:
将180g甲醇加入1L反应釜中,并降温至15℃;将78.6g二氯亚砜在5℃滴加入反应釜中;二氯亚砜滴加完全后将L-丙氨酸加入到反应釜中,保温搅拌60min,再缓慢升温至30℃,搅拌1.5h,再升温至48℃搅拌2h;50℃减压蒸馏,结晶制得82.5g L-丙氨酸甲酯盐酸盐。
取反应底物L-丙氨酸甲酯盐酸盐20g和谷氨酰胺21g溶解在纯水(400mL)中,将100mL XPD-03细胞溶液缓慢加入反应底物中,其中XPD-03细胞在整个反应液中的质量百分比为1.5%,实时监控反应液的pH值,用5M氢氧化钠溶液调节pH值在8,温度保持在25℃。反应过程中取样稀释后用液相色谱法检测生成丙谷二肽量。丙谷二肽开始分解时停止反应。反应液离心后,上清加入终浓度20%甲醇60℃水浴30min,0.5%活性炭搅拌30min,离心得上清反应液625mL,丙谷二肽含量60.4.g/L,转化率92.8%。
实施例4
一种丙谷二肽的制备方法,包括:
取反应底物L-丙氨酸甲酯盐酸盐20g和谷氨酰胺21g溶解在纯水(400mL)中,将80mL XPD-04细胞溶液缓慢加入反应底物中,其中XPD-04细胞在整个反应液中的质量百分比为1.5%,实时监控反应液的pH值,用5M氢氧化钠溶液调节pH值在8,温度保持在25℃。反应过程中取样稀释后用液相色谱法检测生成丙谷二肽量。丙谷二肽开始分解时停止反应。反应液离心后,上清加入终浓度20%甲醇60℃水浴30min,0.5%活性炭搅拌30min,离心得上清反应液600mL,丙谷二肽含量61.5g/L,转化率94.5%。
实施例5
一种丙谷二肽的制备方法,包括:
取反应底物L-丙氨酸甲酯盐酸盐20g和谷氨酰胺21g溶解在纯水(400mL)中,将100mLXPD-05细胞溶液缓慢加入反应底物中,其中XPD-05细胞在整个反应液中的质量百分比为1.5%,实时监控反应液的pH值,调节pH=8,温度保持在25℃。反应过程中取样稀释后用液相色谱法检测生成丙谷二肽量。丙谷二肽开始分解时停止反应。反应液离心后,上清加入终浓度20%甲醇60℃水浴30min,0.5%活性炭搅拌30min,离心得上清反应液625mL,丙谷二肽含量55.8g/L,转化率85.7%。
实施例6
一种丙谷二肽的制备方法,包括:
取反应底物L-丙氨酸甲酯盐酸盐20g和谷氨酰胺21g溶解在纯水(400mL)中,将90mL XPD-06细胞溶液缓慢加入反应底物中,其中XPD-05细胞在整个反应液中的质量百分比为1.5%,实时监控反应液的pH值,用5M氢氧化钠溶液调节pH值在8,温度保持在25℃。反应过程中取样稀释后用液相色谱法检测生成丙谷二肽量。丙谷二肽开始分解时停止反应。反应液离心后,上清加入终浓度20%甲醇60℃水浴30min,0.5%活性炭搅拌30min,离心得上清反应液612.5mL,丙谷二肽含量60.7g/L,转化率93.2%。
实施例7
一种丙谷二肽的制备方法,包括:
取反应底物L-丙氨酸甲酯盐酸盐20g和谷氨酰胺21g溶解在纯水(400mL)中,将50mL XPD-07细胞溶液缓慢加入反应底物中,其中XPD-07细胞在整个反应液中的质量百分比为1.5%,实时监控反应液的pH值,用5M氢氧化钠溶液调节pH值在8,温度保持在25℃。反应过程中取样稀释后用液相色谱法检测生成丙谷二肽量。丙谷二肽开始分解时停止反应。反应液离心后,上清加入终浓度20%甲醇60℃水浴30min,0.5%活性炭搅拌30min,离心得上清反应液562.5mL,丙谷二肽含量60.9g/L,转化率93.5%。
以上所述实施例仅表达了本发明的几种实施方式,其描述较为具体和详细,但并不能因此而理解为对本发明专利范围的限制。应当指出的是,对于本领域的普通技术人员来说,在不脱离本发明构思的前提下,还可以做出若干变形和改进,这些都属于本发明的保护范围。因此,本发明专利的保护范围应以所附权利要求为准。
序列表
<110> 邦泰生物工程(深圳)有限公司
邦泰合盛生物科技(深圳)有限公司
江西安泽麦生物科技有限公司
<120> 丙谷二肽的制备方法、丙谷二肽制备用酶及应用
<130>
<160> 37
<170> PatentIn version 3.5
<210> 1
<211> 617
<212> PRT
<213> 脑膜败血伊丽莎白菌(Elizabethkingia meningoseptica)
<400> 1
Lys Lys Arg Phe Leu Leu Ala Met Ala Met Ala Gly Thr Ala Met Leu
1 5 10 15
Phe Ser Gln Ser Arg Lys Glu Asp Ser Leu Tyr Val Arg Glu Asn Tyr
20 25 30
Thr Lys Ile Glu Lys Leu Ile Pro Met Arg Asp Gly Lys Lys Leu Phe
35 40 45
Thr Ala Ile Tyr Met Pro Lys Asp Gln Lys Gln Lys Tyr Pro Val Leu
50 55 60
Leu Asn Arg Thr Pro Tyr Thr Val Ala Pro Tyr Gly Glu Asn Gln Tyr
65 70 75 80
Lys Leu Ser Leu Gly Asn Phe Pro Ala Glu Met Arg Glu Gly Phe Ile
85 90 95
Phe Val Tyr Gln Asp Val Arg Gly Lys Trp Met Ser Glu Gly Glu Phe
100 105 110
Glu Asp Val Arg Pro Thr Leu Lys Pro Gly Gln Lys Gly Ile Asp Glu
115 120 125
Ser Thr Asp Thr Tyr Asp Thr Leu Glu Trp Leu Ser Lys Asn Leu Lys
130 135 140
Asn Tyr Asn Gln Lys Ala Gly Val Tyr Gly Ile Ser Tyr Pro Gly Phe
145 150 155 160
Tyr Ser Thr Ala Thr Leu Val Asn Ser His Pro Thr Leu Lys Ala Val
165 170 175
Ser Pro Gln Ala Pro Val Thr Asp Trp Tyr Leu Gly Asp Asp Phe His
180 185 190
His Lys Gly Ala Met Phe Leu Asn Asp Ala Phe Met Phe Met Thr Ser
195 200 205
Phe Gly Val Pro Arg Pro Gln Pro Ile Thr Pro Asp Lys Gly Pro Lys
210 215 220
Arg Phe Ile Pro Pro Val Lys Glu Thr Tyr Lys Phe Phe Leu Glu Ser
225 230 235 240
Gly Ser Asn Lys Glu Leu Lys Asp Lys Tyr Met Gly Thr Asn Ile Lys
245 250 255
Phe Phe Asn Asp Met Tyr Ala His Pro Asp Tyr Asp Gln Phe Trp Lys
260 265 270
Asp Arg Asn Ile Leu Pro His Leu Thr Gln Val Lys Pro Ala Val Met
275 280 285
Val Val Gly Gly Phe Phe Asp Ala Glu Asp Ala Tyr Gly Thr Phe Glu
290 295 300
Thr Tyr Lys Ala Ile Glu Lys Gln Asn Pro Lys Ala Asn Asn Ile Leu
305 310 315 320
Val Ala Gly Pro Trp Phe His Gly Gly Trp Val Arg Gly Glu Gly Lys
325 330 335
Gln Phe Gly Asp Ile Leu Phe Gln Lys Pro Thr Ser Ile Asp Tyr Gln
340 345 350
Gln Asn Leu Glu Leu Pro Phe Phe Asn Tyr Tyr Leu Lys Gly Lys Gly
355 360 365
Glu Phe Lys Gly Gly Glu Ala Asn Ile Phe Val Thr Gly Ser Asn Glu
370 375 380
Trp Lys Thr Phe Asp Thr Trp Pro Pro Lys Asn Thr Glu Thr Lys Gln
385 390 395 400
Leu Tyr Phe Gln Pro Asn Gly Lys Leu Ser Phe Asp Lys Val Gln Arg
405 410 415
Thr Asp Ser Trp Asp Glu Tyr Val Ser Asp Pro Asn Lys Pro Val Pro
420 425 430
His Gln Asp Gly Val Gln Thr Ser Arg Thr Arg Glu Tyr Met Ile Asp
435 440 445
Asp Gln Arg Phe Ala Ser Lys Arg Pro Asp Val Met Val Tyr Gln Thr
450 455 460
Asp Ala Leu Gln Glu Asp Ile Thr Leu Val Gly Pro Val Ile Asn His
465 470 475 480
Leu Phe Val Ser Thr Thr Gly Thr Asp Ala Asp Tyr Val Val Lys Leu
485 490 495
Ile Asp Val Tyr Pro Glu Ser Glu Ala Asp Phe Asn Gly Lys Thr Met
500 505 510
Ala Gly Tyr Glu Met Leu Val Arg Gly Glu Ile Met Arg Gly Lys Tyr
515 520 525
Arg Asn Gly Phe Asp Lys Pro Glu Ala Phe Gln Pro Gly Phe Val Thr
530 535 540
Lys Val Asn Tyr Asp Met Pro Asp Ile Ala His Thr Phe Lys Lys Gly
545 550 555 560
His Arg Ile Met Ile Gln Val Gln Asn Ser Trp Phe Pro Leu Ala Asp
565 570 575
Arg Asn Pro Gln Lys Phe Met Asn Ile Tyr Glu Ala Thr Ser Ala Asp
580 585 590
Phe Gln Lys Ala Thr His Arg Ile Phe His Asp Val Lys Asn Pro Thr
595 600 605
Ser Val Glu Val Ser Val Leu Lys Asn
610 615
<210> 2
<211> 617
<212> PRT
<213> 脑膜败血伊丽莎白菌(Elizabethkingia meningoseptica)
<400> 2
Lys Lys Arg Phe Leu Leu Ala Met Ala Met Ala Gly Thr Ala Met Leu
1 5 10 15
Phe Ser Gln Ser Arg Lys Glu Asp Ser Leu Tyr Val Arg Glu Asn Tyr
20 25 30
Thr Lys Ile Glu Lys Leu Ile Pro Met Arg Asp Gly Lys Lys Leu Phe
35 40 45
Thr Ala Ile Tyr Met Pro Lys Asp Gln Lys Gln Lys Tyr Pro Val Leu
50 55 60
Leu Asn Arg Thr Pro Tyr Thr Val Ala Pro Tyr Gly Glu Asn Gln Tyr
65 70 75 80
Lys Leu Ser Leu Gly Asn Phe Pro Ala Glu Met Arg Glu Gly Phe Ile
85 90 95
Phe Val Tyr Gln Asp Val Arg Gly Lys Trp Met Ser Glu Gly Glu Phe
100 105 110
Glu Asp Val Arg Pro Thr Leu Lys Pro Gly Gln Lys Gly Ile Asp Glu
115 120 125
Ser Thr Asp Thr Tyr Asp Thr Leu Glu Trp Leu Ser Lys Asn Leu Lys
130 135 140
Asn Tyr Asn Gln Lys Ala Gly Val Tyr Gly Ile Ser Tyr Pro Gly Phe
145 150 155 160
Tyr Ser Thr Ala Thr Leu Val Asn Ser His Pro Thr Leu Lys Ala Val
165 170 175
Ser Pro Gln Ala Pro Val Thr Asn Trp Tyr Ile Gly Asp Asp Phe His
180 185 190
His Lys Gly Ala Met Phe Leu Asn Asp Ala Phe Met Phe Met Thr Ser
195 200 205
Phe Gly Val Pro Arg Pro Gln Pro Ile Thr Pro Asp Lys Gly Pro Lys
210 215 220
Arg Phe Ile Pro Pro Val Lys Glu Thr Tyr Lys Phe Phe Leu Glu Ser
225 230 235 240
Gly Ser Asn Lys Glu Leu Lys Asp Lys Tyr Met Gly Thr Asn Ile Lys
245 250 255
Phe Phe Asn Asp Met Tyr Ala His Pro Asp Tyr Asp Gln Phe Trp Lys
260 265 270
Asp Arg Asn Ile Leu Pro His Leu Thr Gln Val Lys Pro Ala Val Met
275 280 285
Val Val Gly Gly Phe Phe Asp Ala Glu Asp Ala Tyr Gly Thr Phe Glu
290 295 300
Thr Tyr Lys Ala Ile Glu Lys Gln Asn Pro Lys Ala Asn Asn Ile Leu
305 310 315 320
Val Ala Gly Pro Trp Phe His Gly Gly Trp Val Arg Gly Glu Gly Lys
325 330 335
Gln Phe Gly Asp Ile Leu Phe Gln Lys Pro Thr Ser Ile Asp Tyr Gln
340 345 350
Gln Asn Leu Glu Leu Pro Phe Phe Asn Tyr Tyr Leu Lys Gly Lys Gly
355 360 365
Glu Phe Lys Gly Gly Glu Ala Asn Ile Phe Val Thr Gly Ser Asn Glu
370 375 380
Trp Lys Thr Phe Asp Thr Trp Pro Pro Lys Asn Thr Glu Thr Lys Gln
385 390 395 400
Leu Tyr Phe Gln Pro Asn Gly Lys Leu Ser Phe Asp Lys Val Gln Arg
405 410 415
Thr Asp Ser Trp Asp Glu Tyr Val Ser Asp Pro Asn Lys Pro Val Pro
420 425 430
His Gln Asp Gly Val Gln Thr Ser Arg Thr Arg Glu Tyr Met Ile Asp
435 440 445
Asp Gln Arg Phe Ala Ser Lys Arg Pro Asp Val Met Val Tyr Gln Thr
450 455 460
Asp Ala Leu Gln Glu Asp Ile Thr Leu Val Gly Pro Val Ile Asn His
465 470 475 480
Leu Phe Val Ser Thr Thr Gly Thr Asp Ala Asp Tyr Val Val Lys Leu
485 490 495
Ile Asp Val Tyr Pro Glu Ser Glu Ala Asp Phe Asn Gly Lys Thr Met
500 505 510
Ala Gly Tyr Glu Met Leu Val Arg Gly Glu Ile Met Arg Gly Lys Tyr
515 520 525
Arg Asn Gly Phe Asp Lys Pro Glu Ala Phe Gln Pro Gly Phe Val Thr
530 535 540
Lys Val Asn Tyr Asp Met Pro Asp Ile Ala His Thr Phe Lys Lys Gly
545 550 555 560
His Arg Ile Met Ile Gln Val Gln Asn Ser Trp Phe Pro Leu Ala Asp
565 570 575
Arg Asn Pro Gln Lys Phe Met Asn Ile Tyr Glu Ala Thr Ser Ala Asp
580 585 590
Phe Gln Lys Ala Thr His Arg Ile Phe His Asp Val Lys Asn Pro Thr
595 600 605
Ser Val Glu Val Ser Val Leu Lys Asn
610 615
<210> 3
<211> 617
<212> PRT
<213> 脑膜败血伊丽莎白菌(Elizabethkingia meningoseptica)
<400> 3
Lys Lys Arg Phe Leu Leu Ala Met Ala Met Ala Gly Thr Ala Met Leu
1 5 10 15
Phe Ser Gln Ser Arg Lys Glu Asp Ser Leu Tyr Val Arg Glu Asn Tyr
20 25 30
Thr Lys Ile Glu Lys Leu Ile Pro Met Arg Asp Gly Lys Lys Leu Phe
35 40 45
Thr Ala Ile Tyr Met Pro Lys Asp Gln Lys Gln Lys Tyr Pro Val Leu
50 55 60
Leu Asn Arg Thr Pro Tyr Thr Val Ala Pro Tyr Gly Glu Asn Gln Tyr
65 70 75 80
Lys Leu Ser Leu Gly Asn Phe Pro Ala Glu Met Arg Glu Gly Phe Ile
85 90 95
Phe Val Tyr Gln Asp Val Arg Gly Lys Trp Met Ser Glu Gly Glu Phe
100 105 110
Glu Asp Val Arg Pro Thr Leu Lys Pro Gly Gln Lys Gly Ile Asp Glu
115 120 125
Ser Thr Asp Thr Tyr Asp Thr Leu Glu Trp Leu Ser Lys Asn Leu Lys
130 135 140
Asn Tyr Asn Gln Lys Ala Gly Val Tyr Gly Ile Ser Tyr Pro Gly Phe
145 150 155 160
Tyr Ser Thr Ala Thr Leu Val Asn Ser His Pro Thr Leu Lys Ala Val
165 170 175
Ser Pro Gln Ala Pro Val Thr Asn Trp Tyr Leu Gly Asp Asp Phe His
180 185 190
His Lys Gly Ala Met Phe Leu Asn Asp Ala Phe Met Phe Met Thr Ser
195 200 205
Phe Gly Val Pro Arg Pro Gln Pro Ile Thr Pro Asp Lys Gly Pro Lys
210 215 220
Arg Phe Ile Pro Pro Val Lys Glu Thr Tyr Lys Phe Phe Leu Glu Ser
225 230 235 240
Gly Ser Asn Lys Glu Leu Lys Asp Lys Tyr Met Gly Thr Asn Ile Lys
245 250 255
Phe Phe Asn Asp Met Tyr Ala His Pro Asp Tyr Asp Gln Phe Trp Lys
260 265 270
Asp Arg Asn Ile Leu Pro His Leu Thr Gln Val Lys Pro Ala Val Met
275 280 285
Val Val Gly Gly Phe Phe Asp Ala Glu Asp Ala Tyr Gly Thr Phe Glu
290 295 300
Thr Tyr Lys Ala Ile Glu Lys Gln Asn Pro Lys Ala Asn Asn Ile Leu
305 310 315 320
Val Ala Gly Pro Trp Phe His Gly Gly Trp Val Arg Gly Glu Gly Lys
325 330 335
Gln Phe Gly Asp Ile Leu Phe Gln Lys Pro Thr Ser Ile Asp Tyr Gln
340 345 350
Gln Asn Leu Glu Leu Pro Phe Phe Asn Tyr Tyr Leu Lys Gly Lys Gly
355 360 365
Glu Phe Lys Gly Gly Glu Ala Asn Ile Phe Val Thr Gly Ser Asn Glu
370 375 380
Trp Lys Thr Phe Asp Thr Trp Pro Pro Lys Asn Thr Glu Thr Lys Gln
385 390 395 400
Leu Tyr Phe Gln Pro Asn Gly Lys Leu Ser Phe Asp Lys Val Gln Arg
405 410 415
Thr Asp Ser Trp Asp Glu Tyr Val Ser Asp Pro Asn Lys Pro Val Pro
420 425 430
His Gln Asp Gly Val Gln Thr Ser Arg Thr Arg Glu Tyr Met Ile Asp
435 440 445
Asp Gln Arg Phe Ala Ser Lys Arg Pro Asp Val Lys Thr Tyr Gln Thr
450 455 460
Asp Ala Leu Gln Glu Asp Ile Thr Leu Val Gly Pro Val Ile Asn His
465 470 475 480
Leu Phe Val Ser Thr Thr Gly Thr Asp Ala Asp Tyr Val Val Lys Leu
485 490 495
Ile Asp Val Tyr Pro Glu Ser Glu Ala Asp Phe Asn Gly Lys Thr Met
500 505 510
Ala Gly Tyr Glu Met Leu Val Arg Gly Glu Ile Met Arg Gly Lys Tyr
515 520 525
Arg Asn Gly Phe Asp Lys Pro Glu Ala Phe Gln Pro Gly Phe Val Thr
530 535 540
Lys Val Asn Tyr Asp Met Pro Asp Ile Ala His Thr Phe Lys Lys Gly
545 550 555 560
His Arg Ile Met Ile Gln Val Gln Asn Ser Trp Phe Pro Leu Ala Asp
565 570 575
Arg Asn Pro Gln Lys Phe Met Asn Ile Tyr Glu Ala Thr Ser Ala Asp
580 585 590
Phe Gln Lys Ala Thr His Arg Ile Phe His Asp Val Lys Asn Pro Thr
595 600 605
Ser Val Glu Val Ser Val Leu Lys Asn
610 615
<210> 4
<211> 617
<212> PRT
<213> 脑膜败血伊丽莎白菌(Elizabethkingia meningoseptica)
<400> 4
Lys Lys Arg Phe Leu Leu Ala Met Ala Met Ala Gly Thr Ala Met Leu
1 5 10 15
Phe Ser Gln Ser Arg Lys Glu Asp Ser Leu Tyr Val Arg Glu Asn Tyr
20 25 30
Thr Lys Ile Glu Lys Leu Ile Pro Met Arg Asp Gly Lys Lys Leu Phe
35 40 45
Thr Ala Ile Tyr Met Pro Lys Asp Gln Lys Gln Lys Tyr Pro Val Leu
50 55 60
Leu Asn Arg Thr Pro Tyr Thr Val Ala Pro Tyr Gly Glu Asn Gln Tyr
65 70 75 80
Lys Leu Ser Leu Gly Asn Phe Pro Ala Glu Met Arg Glu Gly Phe Ile
85 90 95
Phe Val Tyr Gln Asp Val Arg Gly Lys Trp Met Ser Glu Gly Glu Phe
100 105 110
Glu Asp Val Arg Pro Thr Leu Lys Pro Gly Gln Lys Gly Ile Asp Glu
115 120 125
Ser Thr Asp Thr Tyr Asp Thr Leu Glu Trp Leu Ser Lys Asn Leu Lys
130 135 140
Asn Tyr Asn Gln Lys Ala Gly Val Tyr Gly Ile Ser Tyr Pro Gly Phe
145 150 155 160
Tyr Ser Thr Ala Thr Leu Val Asn Ser His Pro Thr Leu Lys Ala Val
165 170 175
Ser Pro Gln Ala Pro Val Thr Asn Trp Tyr Leu Gly Asp Asp Phe His
180 185 190
His Lys Gly Ala Met Phe Leu Asn Asp Ala Phe Met Phe Met Thr Ser
195 200 205
Phe Gly Val Pro Arg Pro Gln Pro Ile Thr Pro Asp Lys Gly Pro Lys
210 215 220
Arg Phe Ile Pro Pro Val Lys Glu Thr Tyr Lys Phe Phe Leu Glu Ser
225 230 235 240
Gly Ser Asn Lys Glu Leu Lys Asp Lys Tyr Met Gly Thr Asn Ile Lys
245 250 255
Phe Phe Asn Asp Met Tyr Ala His Pro Asp Tyr Asp Gln Phe Trp Lys
260 265 270
Asp Arg Asn Ile Leu Pro His Leu Thr Gln Val Lys Pro Ala Val Met
275 280 285
Val Val Gly Gly Phe Phe Asp Ala Glu Asp Ala Tyr Gly Thr Phe Glu
290 295 300
Thr Tyr Lys Ala Ile Glu Lys Gln Asn Pro Lys Ala Asn Asn Ile Leu
305 310 315 320
Val Ala Gly Pro Trp Phe His Gly Gly Trp Val Arg Gly Glu Gly Lys
325 330 335
Gln Phe Gly Asp Ile Leu Phe Gln Lys Pro Thr Ser Ile Asp Tyr Gln
340 345 350
Gln Asn Leu Glu Leu Pro Phe Phe Asn Tyr Tyr Leu Lys Gly Lys Gly
355 360 365
Glu Phe Lys Gly Gly Glu Ala Asn Ile Phe Val Thr Gly Ser Asn Glu
370 375 380
Trp Lys Thr Phe Asp Thr Trp Pro Pro Lys Asn Thr Glu Thr Lys Gln
385 390 395 400
Leu Tyr Phe Gln Pro Asn Gly Lys Leu Ser Phe Asp Lys Val Gln Arg
405 410 415
Thr Asp Ser Trp Asp Glu Tyr Val Ser Asp Pro Asn Lys Pro Val Pro
420 425 430
His Gln Asp Gly Val Gln Thr Ser Arg Thr Arg Glu Tyr Met Ile Asp
435 440 445
Asp Gln Arg Phe Ala Ser Lys Arg Pro Asp Val Met Val Tyr Gln Thr
450 455 460
Asp Ala Leu Gln Glu Asp Ile Thr Leu Val Gly Pro Val Ile Asn His
465 470 475 480
Leu Phe Val Ser Thr Thr Gly Thr Asp Ala Asp Tyr Val Val Lys Val
485 490 495
Ile Asp Val Tyr Pro Glu Ser Glu Ala Asp Phe Asn Gly Lys Thr Met
500 505 510
Ala Gly Tyr Glu Met Leu Val Arg Gly Glu Ile Met Arg Gly Lys Tyr
515 520 525
Arg Asn Gly Phe Asp Lys Pro Glu Ala Phe Gln Pro Gly Phe Val Thr
530 535 540
Lys Val Asn Tyr Asp Met Pro Asp Ile Ala His Thr Phe Lys Lys Gly
545 550 555 560
His Arg Ile Met Ile Gln Val Gln Asn Ser Trp Phe Pro Leu Ala Asp
565 570 575
Arg Asn Pro Gln Lys Phe Met Asn Ile Tyr Glu Ala Thr Ser Ala Asp
580 585 590
Phe Gln Lys Ala Thr His Arg Ile Phe His Asp Val Lys Asn Pro Thr
595 600 605
Ser Val Glu Val Ser Val Leu Lys Asn
610 615
<210> 5
<211> 617
<212> PRT
<213> 脑膜败血伊丽莎白菌(Elizabethkingia meningoseptica)
<400> 5
Lys Lys Arg Phe Leu Leu Ala Met Ala Met Ala Gly Thr Ala Met Leu
1 5 10 15
Phe Ser Gln Ser Arg Lys Glu Asp Ser Leu Tyr Val Arg Glu Asn Tyr
20 25 30
Thr Lys Ile Glu Lys Leu Ile Pro Met Arg Asp Gly Lys Lys Leu Phe
35 40 45
Thr Ala Ile Tyr Met Pro Lys Asp Gln Lys Gln Lys Tyr Pro Val Leu
50 55 60
Leu Asn Arg Thr Pro Tyr Thr Val Ala Pro Tyr Gly Glu Asn Gln Tyr
65 70 75 80
Lys Leu Ser Leu Gly Asn Phe Pro Ala Glu Met Arg Glu Gly Phe Ile
85 90 95
Phe Val Tyr Gln Asp Val Arg Gly Lys Trp Met Ser Glu Gly Glu Phe
100 105 110
Glu Asp Val Arg Pro Thr Leu Lys Pro Gly Gln Lys Gly Ile Asp Glu
115 120 125
Ser Thr Asp Thr Tyr Asp Thr Leu Glu Trp Leu Ser Lys Asn Leu Lys
130 135 140
Asn Tyr Asn Gln Lys Ala Gly Val Tyr Gly Ile Ser Tyr Pro Gly Phe
145 150 155 160
Tyr Ser Thr Ala Thr Leu Val Asn Ser His Pro Thr Leu Lys Ala Val
165 170 175
Ser Pro Gln Ala Pro Val Thr Asn Trp Tyr Leu Gly Asp Asp Phe His
180 185 190
His Lys Gly Ala Met Phe Leu Asn Asp Ala Phe Met Phe Met Thr Ser
195 200 205
Phe Gly Val Pro Arg Pro Gln Pro Ile Thr Pro Asp Lys Gly Pro Lys
210 215 220
Arg Phe Ile Pro Pro Val Lys Glu Thr Tyr Lys Phe Phe Leu Glu Ser
225 230 235 240
Gly Ser Asn Lys Glu Leu Lys Asp Lys Tyr Met Gly Thr Asn Ile Lys
245 250 255
Phe Phe Asn Asp Met Tyr Ala His Pro Asp Tyr Asp Gln Phe Trp Lys
260 265 270
Asp Arg Asn Ile Leu Pro His Leu Thr Gln Val Lys Pro Ala Val Met
275 280 285
Val Val Gly Gly Phe Phe Asp Ala Glu Asp Ala Tyr Gly Thr Phe Glu
290 295 300
Thr Tyr Lys Ala Ile Glu Lys Gln Asn Pro Lys Ala Asn Asn Ile Leu
305 310 315 320
Val Ala Gly Pro Trp Phe His Gly Gly Trp Val Arg Gly Glu Gly Lys
325 330 335
Gln Phe Gly Asp Ile Leu Phe Gln Lys Pro Thr Ser Ile Asp Tyr Gln
340 345 350
Gln Asn Leu Glu Leu Pro Phe Phe Asn Tyr Tyr Leu Lys Gly Lys Gly
355 360 365
Glu Phe Lys Gly Gly Glu Ala Asn Ile Phe Val Thr Gly Ser Asn Glu
370 375 380
Trp Lys Thr Phe Asp Thr Trp Pro Pro Lys Asn Thr Glu Thr Lys Gln
385 390 395 400
Leu Tyr Phe Gln Pro Asn Gly Lys Leu Ser Phe Asp Lys Val Gln Arg
405 410 415
Thr Asp Ser Trp Asp Glu Tyr Val Ser Asp Pro Asn Lys Pro Val Pro
420 425 430
His Gln Asp Gly Val Gln Thr Ser Arg Thr Arg Glu Tyr Met Ile Asp
435 440 445
Asp Gln Arg Phe Ala Ser Lys Arg Pro Asp Val Met Val Tyr Gln Thr
450 455 460
Asp Ala Leu Gln Glu Asp Ile Thr Leu Val Gly Pro Val Ile Asn His
465 470 475 480
Leu Phe Val Ser Thr Thr Gly Thr Asp Ala Asp Tyr Val Val Lys Leu
485 490 495
Ile Asp Val Tyr Pro Glu Ser Glu Ala Asp Phe Asn Gly Lys Thr Met
500 505 510
Ala Gly Tyr Glu Met Leu Val Arg Gly Glu Ile Met Arg Gly Lys Tyr
515 520 525
Arg Asn Gly Phe Asp Lys Pro Glu Ala Phe Gln Pro Gly Phe Val Thr
530 535 540
Lys Val Asn Tyr Glu Met Pro Asp Ile Ala His Thr Phe Lys Lys Gly
545 550 555 560
His Arg Ile Met Ile Gln Val Gln Asn Ser Trp Phe Pro Leu Ala Asp
565 570 575
Arg Asn Pro Gln Lys Phe Met Asn Ile Tyr Glu Ala Thr Ser Ala Asp
580 585 590
Phe Gln Lys Ala Thr His Arg Ile Phe His Asp Val Lys Asn Pro Thr
595 600 605
Ser Val Glu Val Ser Val Leu Lys Asn
610 615
<210> 6
<211> 617
<212> PRT
<213> 脑膜败血伊丽莎白菌(Elizabethkingia meningoseptica)
<400> 6
Lys Lys Arg Phe Leu Leu Ala Met Ala Met Ala Gly Thr Ala Met Leu
1 5 10 15
Phe Ser Gln Ser Arg Lys Glu Asp Ser Leu Tyr Val Arg Glu Asn Tyr
20 25 30
Thr Lys Ile Glu Lys Leu Ile Pro Met Arg Asp Gly Lys Lys Leu Phe
35 40 45
Thr Ala Ile Tyr Met Pro Lys Asp Gln Lys Gln Lys Tyr Pro Val Leu
50 55 60
Leu Asn Arg Thr Pro Tyr Thr Val Ala Pro Tyr Gly Glu Asn Gln Tyr
65 70 75 80
Lys Leu Ser Leu Gly Asn Phe Pro Ala Glu Met Arg Glu Gly Phe Ile
85 90 95
Phe Val Tyr Gln Asp Val Arg Gly Lys Trp Met Ser Glu Gly Glu Phe
100 105 110
Glu Asp Val Arg Pro Thr Leu Lys Pro Gly Gln Lys Gly Ile Asp Glu
115 120 125
Ser Thr Asp Thr Tyr Asp Thr Leu Glu Trp Leu Ser Lys Asn Leu Lys
130 135 140
Asn Tyr Asn Gln Lys Ala Gly Val Tyr Gly Ile Ser Tyr Pro Gly Phe
145 150 155 160
Tyr Ser Thr Ala Thr Leu Val Asn Ser His Pro Thr Leu Lys Ala Val
165 170 175
Ser Pro Gln Ala Pro Val Thr Asn Trp Tyr Leu Gly Asp Asp Phe His
180 185 190
His Lys Gly Ala Met Phe Leu Asn Asp Ala Phe Met Phe Met Thr Ser
195 200 205
Phe Gly Val Pro Arg Pro Gln Pro Ile Thr Pro Asp Lys Gly Pro Lys
210 215 220
Arg Phe Ile Pro Pro Val Lys Glu Thr Tyr Lys Phe Phe Leu Glu Ser
225 230 235 240
Gly Ser Asn Lys Glu Leu Lys Asp Lys Tyr Met Gly Thr Asn Ile Lys
245 250 255
Phe Phe Asn Asp Met Tyr Ala His Pro Asp Tyr Asp Gln Phe Trp Lys
260 265 270
Asp Arg Asn Ile Leu Pro His Leu Thr Gln Val Lys Pro Ala Val Met
275 280 285
Val Val Gly Gly Phe Phe Asp Ala Glu Asp Ala Tyr Gly Thr Phe Glu
290 295 300
Thr Tyr Lys Ala Ile Glu Lys Gln Asn Pro Lys Ala Asn Asn Ile Leu
305 310 315 320
Val Ala Gly Pro Trp Phe His Gly Gly Trp Val Arg Gly Glu Gly Lys
325 330 335
Gln Phe Gly Asp Ile Leu Phe Gln Lys Pro Thr Ser Ile Asp Tyr Gln
340 345 350
Gln Asn Leu Glu Leu Pro Phe Phe Asn Tyr Tyr Leu Lys Gly Lys Gly
355 360 365
Glu Phe Lys Gly Gly Glu Ala Asn Ile Phe Val Thr Gly Ser Asn Glu
370 375 380
Trp Lys Thr Phe Asp Thr Trp Pro Pro Lys Asn Thr Glu Thr Lys Gln
385 390 395 400
Leu Tyr Phe Gln Pro Asn Gly Lys Leu Ser Phe Asp Lys Val Gln Arg
405 410 415
Thr Asp Ser Trp Asp Glu Tyr Val Ser Asp Pro Asn Lys Pro Val Pro
420 425 430
His Gln Asp Gly Val Gln Thr Ser Arg Thr Arg Glu Tyr Met Ile Asp
435 440 445
Asp Gln Arg Phe Ala Ser Lys Arg Pro Asp Val Met Val Tyr Gln Thr
450 455 460
Asp Ala Leu Gln Glu Asp Ile Thr Leu Val Gly Pro Val Ile Asn His
465 470 475 480
Leu Phe Val Ser Thr Thr Gly Thr Asp Ala Asp Tyr Val Val Lys Leu
485 490 495
Ile Asp Val Tyr Pro Glu Ser Glu Ala Asp Phe Asn Gly Lys Thr Met
500 505 510
Ala Gly Tyr Glu Met Leu Val Arg Gly Glu Ile Met Arg Gly Lys Tyr
515 520 525
Arg Asn Gly Phe Asp Lys Pro Glu Ala Phe Gln Pro Gly Phe Val Thr
530 535 540
Lys Val Asn Tyr Asp Met Pro Asp Ile Ala His Thr Phe Lys Lys Gly
545 550 555 560
His Arg Ile Met Ile Gln Ile Gln Asn Ser Trp Phe Pro Leu Ala Asp
565 570 575
Arg Asn Pro Gln Lys Phe Met Asn Ile Tyr Glu Ala Thr Ser Ala Asp
580 585 590
Phe Gln Lys Ala Thr His Arg Ile Phe His Asp Val Lys Asn Pro Thr
595 600 605
Ser Val Glu Val Ser Val Leu Lys Asn
610 615
<210> 7
<211> 1851
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
aagaagcgct ttctgctggc aatggccatg gcaggcaccg caatgctgtt tagtcagagt 60
cgtaaagaag atagcctgta tgtgcgcgaa aattatacca aaattgaaaa actgatcccg 120
atgcgcgatg gtaaaaaact gtttaccgcc atctatatgc cgaaagatca gaaacagaaa 180
tatccggttc tgctgaatcg taccccgtat accgtggcac cgtatggtga aaatcagtat 240
aaactgagtc tgggtaattt tccggccgaa atgcgtgaag gttttatttt tgtttaccag 300
gatgttcgtg gtaaatggat gagtgaaggc gaatttgaag atgttcgtcc gaccctgaaa 360
ccgggtcaga aaggcattga tgaaagcacc gatacctatg ataccctgga atggctgagc 420
aaaaatctga aaaattataa ccagaaggcc ggcgtttatg gtattagtta tccgggtttt 480
tatagtaccg ccaccctggt gaatagccat ccgaccctga aggcagttag cccgcaggcc 540
ccggttaccg attggtatct gggtgacgat tttcatcata aaggcgcaat gtttctgaat 600
gatgccttta tgtttatgac cagctttggc gttccgcgtc cgcagccgat taccccggat 660
aaaggtccga aacgttttat tccgccggtg aaagaaacct ataaattttt cctggaaagc 720
ggcagtaata aggaactgaa agataaatat atgggcacca atattaagtt cttcaatgat 780
atgtacgccc atccggatta tgatcagttt tggaaagatc gtaatatcct gccgcatctg 840
acccaggtta aaccggcagt tatggtggtg ggtggctttt tcgatgccga agatgcatat 900
ggtacctttg aaacctataa ggcaattgaa aaacagaatc cgaaagccaa taatattctg 960
gtggcaggtc cgtggtttca tggcggctgg gttcgtggtg aaggcaaaca gtttggcgat 1020
attctgtttc agaaaccgac cagtattgat tatcagcaga atctggaact gccgtttttc 1080
aattattatc tgaaaggtaa aggtgagttc aaaggcggtg aagcaaatat ttttgtgacc 1140
ggtagtaatg aatggaaaac ctttgatacc tggccgccga aaaataccga aaccaaacag 1200
ctgtattttc agccgaatgg caaactgagc tttgataaag ttcagcgtac cgatagctgg 1260
gatgaatatg ttagcgatcc gaataagccg gttccgcatc aggatggcgt gcagaccagt 1320
cgcacccgcg aatatatgat tgatgatcag cgttttgcaa gtaaacgccc ggatgttatg 1380
gtttatcaga ccgatgccct gcaggaagat attaccctgg tgggcccggt tattaatcat 1440
ctgtttgtga gcaccaccgg caccgatgcc gattatgttg tgaaactgat tgatgtgtat 1500
ccggaaagcg aagcagattt taatggtaaa accatggccg gttatgaaat gctggttcgt 1560
ggcgaaatta tgcgtggcaa atatcgcaat ggttttgata aaccggaagc atttcagccg 1620
ggctttgtta ccaaagtgaa ttatgatatg ccggatattg cccatacctt taaaaaaggc 1680
catcgtatta tgattcaggt tcagaatagt tggtttccgc tggcagatcg caatccgcag 1740
aaattcatga atatctatga agcaaccagc gcagattttc agaaagccac ccatcgcatt 1800
tttcatgatg ttaaaaatcc gaccagcgtt gaagttagtg tgctgaaaaa t 1851
<210> 8
<211> 1851
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
aagaagcgct ttctgctggc aatggccatg gcaggcaccg caatgctgtt tagtcagagt 60
cgtaaagaag atagcctgta tgtgcgcgaa aattatacca aaattgaaaa actgatcccg 120
atgcgcgatg gtaaaaaact gtttaccgcc atctatatgc cgaaagatca gaaacagaaa 180
tatccggttc tgctgaatcg taccccgtat accgtggcac cgtatggtga aaatcagtat 240
aaactgagtc tgggtaattt tccggccgaa atgcgtgaag gttttatttt tgtttaccag 300
gatgttcgtg gtaaatggat gagtgaaggc gaatttgaag atgttcgtcc gaccctgaaa 360
ccgggtcaga aaggcattga tgaaagcacc gatacctatg ataccctgga atggctgagc 420
aaaaatctga aaaattataa ccagaaggcc ggcgtttatg gtattagtta tccgggtttt 480
tatagtaccg ccaccctggt gaatagccat ccgaccctga aggcagttag cccgcaggcc 540
ccggttacca attggtatat cggtgacgat tttcatcata aaggcgcaat gtttctgaat 600
gatgccttta tgtttatgac cagctttggc gttccgcgtc cgcagccgat taccccggat 660
aaaggtccga aacgttttat tccgccggtg aaagaaacct ataaattttt cctggaaagc 720
ggcagtaata aggaactgaa agataaatat atgggcacca atattaagtt cttcaatgat 780
atgtacgccc atccggatta tgatcagttt tggaaagatc gtaatatcct gccgcatctg 840
acccaggtta aaccggcagt tatggtggtg ggtggctttt tcgatgccga agatgcatat 900
ggtacctttg aaacctataa ggcaattgaa aaacagaatc cgaaagccaa taatattctg 960
gtggcaggtc cgtggtttca tggcggctgg gttcgtggtg aaggcaaaca gtttggcgat 1020
attctgtttc agaaaccgac cagtattgat tatcagcaga atctggaact gccgtttttc 1080
aattattatc tgaaaggtaa aggtgagttc aaaggcggtg aagcaaatat ttttgtgacc 1140
ggtagtaatg aatggaaaac ctttgatacc tggccgccga aaaataccga aaccaaacag 1200
ctgtattttc agccgaatgg caaactgagc tttgataaag ttcagcgtac cgatagctgg 1260
gatgaatatg ttagcgatcc gaataagccg gttccgcatc aggatggcgt gcagaccagt 1320
cgcacccgcg aatatatgat tgatgatcag cgttttgcaa gtaaacgccc ggatgttatg 1380
gtttatcaga ccgatgccct gcaggaagat attaccctgg tgggcccggt tattaatcat 1440
ctgtttgtga gcaccaccgg caccgatgcc gattatgttg tgaaactgat tgatgtgtat 1500
ccggaaagcg aagcagattt taatggtaaa accatggccg gttatgaaat gctggttcgt 1560
ggcgaaatta tgcgtggcaa atatcgcaat ggttttgata aaccggaagc atttcagccg 1620
ggctttgtta ccaaagtgaa ttatgatatg ccggatattg cccatacctt taaaaaaggc 1680
catcgtatta tgattcaggt tcagaatagt tggtttccgc tggcagatcg caatccgcag 1740
aaattcatga atatctatga agcaaccagc gcagattttc agaaagccac ccatcgcatt 1800
tttcatgatg ttaaaaatcc gaccagcgtt gaagttagtg tgctgaaaaa t 1851
<210> 9
<211> 1851
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
aagaagcgct ttctgctggc aatggccatg gcaggcaccg caatgctgtt tagtcagagt 60
cgtaaagaag atagcctgta tgtgcgcgaa aattatacca aaattgaaaa actgatcccg 120
atgcgcgatg gtaaaaaact gtttaccgcc atctatatgc cgaaagatca gaaacagaaa 180
tatccggttc tgctgaatcg taccccgtat accgtggcac cgtatggtga aaatcagtat 240
aaactgagtc tgggtaattt tccggccgaa atgcgtgaag gttttatttt tgtttaccag 300
gatgttcgtg gtaaatggat gagtgaaggc gaatttgaag atgttcgtcc gaccctgaaa 360
ccgggtcaga aaggcattga tgaaagcacc gatacctatg ataccctgga atggctgagc 420
aaaaatctga aaaattataa ccagaaggcc ggcgtttatg gtattagtta tccgggtttt 480
tatagtaccg ccaccctggt gaatagccat ccgaccctga aggcagttag cccgcaggcc 540
ccggttacca attggtatct gggtgacgat tttcatcata aaggcgcaat gtttctgaat 600
gatgccttta tgtttatgac cagctttggc gttccgcgtc cgcagccgat taccccggat 660
aaaggtccga aacgttttat tccgccggtg aaagaaacct ataaattttt cctggaaagc 720
ggcagtaata aggaactgaa agataaatat atgggcacca atattaagtt cttcaatgat 780
atgtacgccc atccggatta tgatcagttt tggaaagatc gtaatatcct gccgcatctg 840
acccaggtta aaccggcagt tatggtggtg ggtggctttt tcgatgccga agatgcatat 900
ggtacctttg aaacctataa ggcaattgaa aaacagaatc cgaaagccaa taatattctg 960
gtggcaggtc cgtggtttca tggcggctgg gttcgtggtg aaggcaaaca gtttggcgat 1020
attctgtttc agaaaccgac cagtattgat tatcagcaga atctggaact gccgtttttc 1080
aattattatc tgaaaggtaa aggtgagttc aaaggcggtg aagcaaatat ttttgtgacc 1140
ggtagtaatg aatggaaaac ctttgatacc tggccgccga aaaataccga aaccaaacag 1200
ctgtattttc agccgaatgg caaactgagc tttgataaag ttcagcgtac cgatagctgg 1260
gatgaatatg ttagcgatcc gaataagccg gttccgcatc aggatggcgt gcagaccagt 1320
cgcacccgcg aatatatgat tgatgatcag cgttttgcaa gtaaacgccc ggatgttaag 1380
acttatcaga ccgatgccct gcaggaagat attaccctgg tgggcccggt tattaatcat 1440
ctgtttgtga gcaccaccgg caccgatgcc gattatgttg tgaaactgat tgatgtgtat 1500
ccggaaagcg aagcagattt taatggtaaa accatggccg gttatgaaat gctggttcgt 1560
ggcgaaatta tgcgtggcaa atatcgcaat ggttttgata aaccggaagc atttcagccg 1620
ggctttgtta ccaaagtgaa ttatgatatg ccggatattg cccatacctt taaaaaaggc 1680
catcgtatta tgattcaggt tcagaatagt tggtttccgc tggcagatcg caatccgcag 1740
aaattcatga atatctatga agcaaccagc gcagattttc agaaagccac ccatcgcatt 1800
tttcatgatg ttaaaaatcc gaccagcgtt gaagttagtg tgctgaaaaa t 1851
<210> 10
<211> 1851
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
aagaagcgct ttctgctggc aatggccatg gcaggcaccg caatgctgtt tagtcagagt 60
cgtaaagaag atagcctgta tgtgcgcgaa aattatacca aaattgaaaa actgatcccg 120
atgcgcgatg gtaaaaaact gtttaccgcc atctatatgc cgaaagatca gaaacagaaa 180
tatccggttc tgctgaatcg taccccgtat accgtggcac cgtatggtga aaatcagtat 240
aaactgagtc tgggtaattt tccggccgaa atgcgtgaag gttttatttt tgtttaccag 300
gatgttcgtg gtaaatggat gagtgaaggc gaatttgaag atgttcgtcc gaccctgaaa 360
ccgggtcaga aaggcattga tgaaagcacc gatacctatg ataccctgga atggctgagc 420
aaaaatctga aaaattataa ccagaaggcc ggcgtttatg gtattagtta tccgggtttt 480
tatagtaccg ccaccctggt gaatagccat ccgaccctga aggcagttag cccgcaggcc 540
ccggttacca attggtatct gggtgacgat tttcatcata aaggcgcaat gtttctgaat 600
gatgccttta tgtttatgac cagctttggc gttccgcgtc cgcagccgat taccccggat 660
aaaggtccga aacgttttat tccgccggtg aaagaaacct ataaattttt cctggaaagc 720
ggcagtaata aggaactgaa agataaatat atgggcacca atattaagtt cttcaatgat 780
atgtacgccc atccggatta tgatcagttt tggaaagatc gtaatatcct gccgcatctg 840
acccaggtta aaccggcagt tatggtggtg ggtggctttt tcgatgccga agatgcatat 900
ggtacctttg aaacctataa ggcaattgaa aaacagaatc cgaaagccaa taatattctg 960
gtggcaggtc cgtggtttca tggcggctgg gttcgtggtg aaggcaaaca gtttggcgat 1020
attctgtttc agaaaccgac cagtattgat tatcagcaga atctggaact gccgtttttc 1080
aattattatc tgaaaggtaa aggtgagttc aaaggcggtg aagcaaatat ttttgtgacc 1140
ggtagtaatg aatggaaaac ctttgatacc tggccgccga aaaataccga aaccaaacag 1200
ctgtattttc agccgaatgg caaactgagc tttgataaag ttcagcgtac cgatagctgg 1260
gatgaatatg ttagcgatcc gaataagccg gttccgcatc aggatggcgt gcagaccagt 1320
cgcacccgcg aatatatgat tgatgatcag cgttttgcaa gtaaacgccc ggatgttatg 1380
gtttatcaga ccgatgccct gcaggaagat attaccctgg tgggcccggt tattaatcat 1440
ctgtttgtga gcaccaccgg caccgatgcc gattatgttg tgaaagtgat tgatgtgtat 1500
ccggaaagcg aagcagattt taatggtaaa accatggccg gttatgaaat gctggttcgt 1560
ggcgaaatta tgcgtggcaa atatcgcaat ggttttgata aaccggaagc atttcagccg 1620
ggctttgtta ccaaagtgaa ttatgatatg ccggatattg cccatacctt taaaaaaggc 1680
catcgtatta tgattcaggt tcagaatagt tggtttccgc tggcagatcg caatccgcag 1740
aaattcatga atatctatga agcaaccagc gcagattttc agaaagccac ccatcgcatt 1800
tttcatgatg ttaaaaatcc gaccagcgtt gaagttagtg tgctgaaaaa t 1851
<210> 11
<211> 1851
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 11
aagaagcgct ttctgctggc aatggccatg gcaggcaccg caatgctgtt tagtcagagt 60
cgtaaagaag atagcctgta tgtgcgcgaa aattatacca aaattgaaaa actgatcccg 120
atgcgcgatg gtaaaaaact gtttaccgcc atctatatgc cgaaagatca gaaacagaaa 180
tatccggttc tgctgaatcg taccccgtat accgtggcac cgtatggtga aaatcagtat 240
aaactgagtc tgggtaattt tccggccgaa atgcgtgaag gttttatttt tgtttaccag 300
gatgttcgtg gtaaatggat gagtgaaggc gaatttgaag atgttcgtcc gaccctgaaa 360
ccgggtcaga aaggcattga tgaaagcacc gatacctatg ataccctgga atggctgagc 420
aaaaatctga aaaattataa ccagaaggcc ggcgtttatg gtattagtta tccgggtttt 480
tatagtaccg ccaccctggt gaatagccat ccgaccctga aggcagttag cccgcaggcc 540
ccggttacca attggtatct gggtgacgat tttcatcata aaggcgcaat gtttctgaat 600
gatgccttta tgtttatgac cagctttggc gttccgcgtc cgcagccgat taccccggat 660
aaaggtccga aacgttttat tccgccggtg aaagaaacct ataaattttt cctggaaagc 720
ggcagtaata aggaactgaa agataaatat atgggcacca atattaagtt cttcaatgat 780
atgtacgccc atccggatta tgatcagttt tggaaagatc gtaatatcct gccgcatctg 840
acccaggtta aaccggcagt tatggtggtg ggtggctttt tcgatgccga agatgcatat 900
ggtacctttg aaacctataa ggcaattgaa aaacagaatc cgaaagccaa taatattctg 960
gtggcaggtc cgtggtttca tggcggctgg gttcgtggtg aaggcaaaca gtttggcgat 1020
attctgtttc agaaaccgac cagtattgat tatcagcaga atctggaact gccgtttttc 1080
aattattatc tgaaaggtaa aggtgagttc aaaggcggtg aagcaaatat ttttgtgacc 1140
ggtagtaatg aatggaaaac ctttgatacc tggccgccga aaaataccga aaccaaacag 1200
ctgtattttc agccgaatgg caaactgagc tttgataaag ttcagcgtac cgatagctgg 1260
gatgaatatg ttagcgatcc gaataagccg gttccgcatc aggatggcgt gcagaccagt 1320
cgcacccgcg aatatatgat tgatgatcag cgttttgcaa gtaaacgccc ggatgttatg 1380
gtttatcaga ccgatgccct gcaggaagat attaccctgg tgggcccggt tattaatcat 1440
ctgtttgtga gcaccaccgg caccgatgcc gattatgttg tgaaactgat tgatgtgtat 1500
ccggaaagcg aagcagattt taatggtaaa accatggccg gttatgaaat gctggttcgt 1560
ggcgaaatta tgcgtggcaa atatcgcaat ggttttgata aaccggaagc atttcagccg 1620
ggctttgtta ccaaagtgaa ttatgagatg ccggatattg cccatacctt taaaaaaggc 1680
catcgtatta tgattcaggt tcagaatagt tggtttccgc tggcagatcg caatccgcag 1740
aaattcatga atatctatga agcaaccagc gcagattttc agaaagccac ccatcgcatt 1800
tttcatgatg ttaaaaatcc gaccagcgtt gaagttagtg tgctgaaaaa t 1851
<210> 12
<211> 1851
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 12
aagaagcgct ttctgctggc aatggccatg gcaggcaccg caatgctgtt tagtcagagt 60
cgtaaagaag atagcctgta tgtgcgcgaa aattatacca aaattgaaaa actgatcccg 120
atgcgcgatg gtaaaaaact gtttaccgcc atctatatgc cgaaagatca gaaacagaaa 180
tatccggttc tgctgaatcg taccccgtat accgtggcac cgtatggtga aaatcagtat 240
aaactgagtc tgggtaattt tccggccgaa atgcgtgaag gttttatttt tgtttaccag 300
gatgttcgtg gtaaatggat gagtgaaggc gaatttgaag atgttcgtcc gaccctgaaa 360
ccgggtcaga aaggcattga tgaaagcacc gatacctatg ataccctgga atggctgagc 420
aaaaatctga aaaattataa ccagaaggcc ggcgtttatg gtattagtta tccgggtttt 480
tatagtaccg ccaccctggt gaatagccat ccgaccctga aggcagttag cccgcaggcc 540
ccggttacca attggtatct gggtgacgat tttcatcata aaggcgcaat gtttctgaat 600
gatgccttta tgtttatgac cagctttggc gttccgcgtc cgcagccgat taccccggat 660
aaaggtccga aacgttttat tccgccggtg aaagaaacct ataaattttt cctggaaagc 720
ggcagtaata aggaactgaa agataaatat atgggcacca atattaagtt cttcaatgat 780
atgtacgccc atccggatta tgatcagttt tggaaagatc gtaatatcct gccgcatctg 840
acccaggtta aaccggcagt tatggtggtg ggtggctttt tcgatgccga agatgcatat 900
ggtacctttg aaacctataa ggcaattgaa aaacagaatc cgaaagccaa taatattctg 960
gtggcaggtc cgtggtttca tggcggctgg gttcgtggtg aaggcaaaca gtttggcgat 1020
attctgtttc agaaaccgac cagtattgat tatcagcaga atctggaact gccgtttttc 1080
aattattatc tgaaaggtaa aggtgagttc aaaggcggtg aagcaaatat ttttgtgacc 1140
ggtagtaatg aatggaaaac ctttgatacc tggccgccga aaaataccga aaccaaacag 1200
ctgtattttc agccgaatgg caaactgagc tttgataaag ttcagcgtac cgatagctgg 1260
gatgaatatg ttagcgatcc gaataagccg gttccgcatc aggatggcgt gcagaccagt 1320
cgcacccgcg aatatatgat tgatgatcag cgttttgcaa gtaaacgccc ggatgttatg 1380
gtttatcaga ccgatgccct gcaggaagat attaccctgg tgggcccggt tattaatcat 1440
ctgtttgtga gcaccaccgg caccgatgcc gattatgttg tgaaactgat tgatgtgtat 1500
ccggaaagcg aagcagattt taatggtaaa accatggccg gttatgaaat gctggttcgt 1560
ggcgaaatta tgcgtggcaa atatcgcaat ggttttgata aaccggaagc atttcagccg 1620
ggctttgtta ccaaagtgaa ttatgatatg ccggatattg cccatacctt taaaaaaggc 1680
catcgtatta tgattcagat tcagaatagt tggtttccgc tggcagatcg caatccgcag 1740
aaattcatga atatctatga agcaaccagc gcagattttc agaaagccac ccatcgcatt 1800
tttcatgatg ttaaaaatcc gaccagcgtt gaagttagtg tgctgaaaaa t 1851
<210> 13
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 13
ttctctagag tcgacggctt aatggcagga taagc 35
<210> 14
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 14
ccatgccttc tgaaggcgat gacttcgtgt taatc 35
<210> 15
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 15
acacgaagtc atcgccttca gaaggcatgg atgag 35
<210> 16
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 16
cagggtaata gatctccata tcggcaaaat tggac 35
<210> 17
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 17
cagtacaagc gttttagagc tagaaatagc aag 33
<210> 18
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 18
acgctcatcc actagtatta tacctaggac tgagc 35
<210> 19
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 19
ttctctagag tcgacgtgga ccgatagccg cgaaa 35
<210> 20
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 20
ttgaacgcat tgccgctgcc ccgttcaaat gcagg 35
<210> 21
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 21
catttgaacg gggcagcggc aatgcgttca agctggg 37
<210> 22
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 22
cagggtaata gatctgacca ctgttcaacc ggcgc 35
<210> 23
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 23
ggtgaaggct gttttagagc tagaaatagc aag 33
<210> 24
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 24
gcttaactga actagtatta tacctaggac tgagc 35
<210> 25
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 25
ttctctagag tcgacgcact ttaagctcgc gctggt 36
<210> 26
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 26
accgttcggg gtggccgatc acccgcaaaa cagtattgc 39
<210> 27
<211> 38
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 27
ttttgcgggt gatcggccac cccgaacggt gtgattcg 38
<210> 28
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 28
cagggtaata gatcgtggac cggtgatagt tggcccg 37
<210> 29
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 29
gtcgtcttac gttttagagc tagaaatagc aag 33
<210> 30
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 30
cggtttacga actagtatta tacctaggac tgagc 35
<210> 31
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 31
ttctctagag tcgacccgcc gcgacatgca catcg 35
<210> 32
<211> 36
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 32
ggaaccagtc gcgacaggac agcctggctg accgcg 36
<210> 33
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 33
gtcagccagg ctgcctgtcg cgactggttc cagc 34
<210> 34
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 34
cagggtaata gatctgcgtg gctggcgtgc gctgg 35
<210> 35
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 35
tggaccgccg ttttagagct agaaatagca ag 32
<210> 36
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 36
ggctcatcat actagtatta tacctaggac tgagc 35
<210> 37
<211> 1851
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 37
aagaagcgct ttctgctggc aatggccatg gcaggcaccg caatgctgtt tagtcagagt 60
cgtaaagaag atagcctgta tgtgcgcgaa aattatacca aaattgaaaa actgatcccg 120
atgcgcgatg gtaaaaaact gtttaccgcc atctatatgc cgaaagatca gaaacagaaa 180
tatccggttc tgctgaatcg taccccgtat accgtggcac cgtatggtga aaatcagtat 240
aaactgagtc tgggtaattt tccggccgaa atgcgtgaag gttttatttt tgtttaccag 300
gatgttcgtg gtaaatggat gagtgaaggc gaatttgaag atgttcgtcc gaccctgaaa 360
ccgggtcaga aaggcattga tgaaagcacc gatacctatg ataccctgga atggctgagc 420
aaaaatctga aaaattataa ccagaaggcc ggcgtttatg gtattagtta tccgggtttt 480
tatagtaccg ccaccctggt gaatagccat ccgaccctga aggcagttag cccgcaggcc 540
ccggttacca attggtatct gggtgacgat tttcatcata aaggcgcaat gtttctgaat 600
gatgccttta tgtttatgac cagctttggc gttccgcgtc cgcagccgat taccccggat 660
aaaggtccga aacgttttat tccgccggtg aaagaaacct ataaattttt cctggaaagc 720
ggcagtaata aggaactgaa agataaatat atgggcacca atattaagtt cttcaatgat 780
atgtacgccc atccggatta tgatcagttt tggaaagatc gtaatatcct gccgcatctg 840
acccaggtta aaccggcagt tatggtggtg ggtggctttt tcgatgccga agatgcatat 900
ggtacctttg aaacctataa ggcaattgaa aaacagaatc cgaaagccaa taatattctg 960
gtggcaggtc cgtggtttca tggcggctgg gttcgtggtg aaggcaaaca gtttggcgat 1020
attctgtttc agaaaccgac cagtattgat tatcagcaga atctggaact gccgtttttc 1080
aattattatc tgaaaggtaa aggtgagttc aaaggcggtg aagcaaatat ttttgtgacc 1140
ggtagtaatg aatggaaaac ctttgatacc tggccgccga aaaataccga aaccaaacag 1200
ctgtattttc agccgaatgg caaactgagc tttgataaag ttcagcgtac cgatagctgg 1260
gatgaatatg ttagcgatcc gaataagccg gttccgcatc aggatggcgt gcagaccagt 1320
cgcacccgcg aatatatgat tgatgatcag cgttttgcaa gtaaacgccc ggatgttatg 1380
gtttatcaga ccgatgccct gcaggaagat attaccctgg tgggcccggt tattaatcat 1440
ctgtttgtga gcaccaccgg caccgatgcc gattatgttg tgaaactgat tgatgtgtat 1500
ccggaaagcg aagcagattt taatggtaaa accatggccg gttatgaaat gctggttcgt 1560
ggcgaaatta tgcgtggcaa atatcgcaat ggttttgata aaccggaagc atttcagccg 1620
ggctttgtta ccaaagtgaa ttatgatatg ccggatattg cccatacctt taaaaaaggc 1680
catcgtatta tgattcaggt tcagaatagt tggtttccgc tggcagatcg caatccgcag 1740
aaattcatga atatctatga agcaaccagc gcagattttc agaaagccac ccatcgcatt 1800
tttcatgatg ttaaaaatcc gaccagcgtt gaagttagtg tgctgaaaaa t 1851