CN110358858A - A kind of method and primer of mating type that identifying spot jade gill fungus White strain monocaryon - Google Patents
A kind of method and primer of mating type that identifying spot jade gill fungus White strain monocaryon Download PDFInfo
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- CN110358858A CN110358858A CN201910689443.9A CN201910689443A CN110358858A CN 110358858 A CN110358858 A CN 110358858A CN 201910689443 A CN201910689443 A CN 201910689443A CN 110358858 A CN110358858 A CN 110358858A
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Abstract
A kind of method and primer of mating type that identifying spot jade gill fungus White strain monocaryon, extract the genomic DNA of spot jade gill fungus white mononuclear bacterial strain, then PCR amplification is carried out with primer HM1F/1R, HM2F/2R and HM3F/3R, if cannot be considered as A with primer amplified shaping bandXBXMating type bacterial strain, if can be considered as AyBy mating type bacterial strain with primer amplified shaping band.Method of the invention is used to identify the mating type of spot jade gill fungus White strain monocaryon bacterial strain, has time-consuming short, easy to operate, precision height, advantage at low cost.
Description
Technical field
The invention belongs to edible mushroom molecular identification technical fields, and in particular to a kind of identification spot jade gill fungus White strain monocaryon
Mating type method and primer.
Background technique
Spot jade gill fungus (Hypsizygusmarmoreus) it is a kind of rare rare saprophytic edible fungus of large size wood in north temperate zone,
Also known as: true pleurotus cornucopiae, false matsutake, spot dried mushroom, Pholiota nameko (t.Ito) S. Ito et Imai, crab flavour mushroom, letter happiness mushroom etc..Spot jade gill fungus is under the jurisdiction of load on classification position
Bacterium door (Basidiomycota), Hymenomycetes (Hymenomycetes), Agaricales (Agaricales), Tricholomataceae
(Tricholomataceae), beautiful gill fungus category (Hypsizygus).Spot jade gill fungus is rich in protein, insatiable hunger as food medicine dual-purpose fungi
With fatty acid, amino acid, crude fibre, carbohydrate, vitamin etc.；There is also various active substances simultaneously, such as: ribosomes inhibits egg
White, collagen binding protein, polysaccharide and angiotensin converting enzyme inhibitor etc..Spot jade gill fungus is as the factorial production skill in recent years
Art one of edible fungus variety the most mature, it is deep to be liked by the people of various countries.
With the continuous development and growth of current domestic spot jade gill fungus the factorial production, industrialized mechanical equipment and advanced
Technical matters plays main function wherein, still, since the spot jade gill fungus microorganism resource in China researches and develops weaker, Some Domestic
Larger enterprise depends on external (Japan, South Korea) to introduce strain for a long time.And the strain quantity of China's independent research
It is also very limited, so far without the kind of entirely appropriate domestic production.
And different genotype and ecotypic monocaryon parent are mainly carried out hybridization pairing by common breeding process, then
Robustness and productivity verifying are carried out to dicaryon hybridization after pairing with clamp connection, due to the gene type of monocaryon
It is difficult to determine, and clamp connection is observed after hybridization complexity and the lower reason of efficiency, so that the hybridization of spot jade gill fungus white is educated
The cumbersome time and effort consuming of kind.
Summary of the invention
The purpose of the present invention is to provide the methods and primer of a kind of mating type for identifying spot jade gill fungus White strain monocaryon.
In order to achieve the above objects and other related objects, present invention provide the technical scheme that a kind of identification spot jade gill fungus is white
The primer of color bacterial strain monocaryon, it is characterised in that: including 3 groups of primer pairs, the nucleotide sequence of first group of primer pair is as follows:
HM1F: 5'- ACCTCTACTTCATCCTTGC-3'；
HM1R: 5'- TCTTGGGCGTCCGTTT-3'；
The nucleotide sequence of second group of primer pair is as follows:
HM2F: 5'-CGTCCCAGATATTCAACTC-3'；
HM2R: 5'-GAAACCTCCAACGCACT-3'；
The nucleotide sequence of third group primer pair is as follows:
HM3F: 5'-CTGGCGTTACCTTCCC-3'；
HM3R: 5’-GTGGTTGTGGCGTGAT-3’。
In order to achieve the above objects and other related objects, present invention provide the technical scheme that a kind of identification spot jade gill fungus is white
The method of color bacterial strain monocaryon, including 3 groups of primer pairs, the nucleotide sequence of first group of primer pair are as follows:
HM1F: 5'- ACCTCTACTTCATCCTTGC-3'；
HM1R: 5'- TCTTGGGCGTCCGTTT-3'；
The nucleotide sequence of second group of primer pair is as follows:
HM2F: 5'-CGTCCCAGATATTCAACTC-3'；
HM2R: 5'-GAAACCTCCAACGCACT-3'；
The nucleotide sequence of third group primer pair is as follows:
HM3F: 5'-CTGGCGTTACCTTCCC-3'；
HM3R: 5'-GTGGTTGTGGCGTGAT-3'；
The method of identification spot jade gill fungus White strain monocaryon includes: to extract the genomic DNA of spot jade gill fungus white mononuclear bacterial strain, then
PCR amplification is carried out with first group of primer pair, second group of primer pair and third group primer pair respectively, the product that PCR amplification comes out is used
The agarose gel electrophoresis of 1%-2% detects；If cannot be considered as AxBx mating type with primer amplified shaping band
Bacterial strain, if can be considered as AyBy mating type bacterial strain with primer amplified shaping band.
Preferred technical solution are as follows: the method for extracting the genomic DNA of spot jade gill fungus White strain monocaryon includes following step
It is rapid: a, to cultivate spot jade gill fungus White strain monocaryon in PDA plate culture medium；B, picking mycelia is placed on after culture
In DNA lysate, after 75-85 DEG C of warm bath 5-10min to obtain the final product.
Preferred technical solution are as follows: the system of pcr amplification reaction are as follows: 150ng/ul spot jade gill fungus White strain monocaryon
DNA2ul, 10umol/L primer 2 ul, 0.1mmol/LdNTP 2 ul, high fidelity enzyme Pfu0.5ul, 5ul loading
Buffer uses ddH2O polishing 25ul.
Preferred technical solution are as follows: the condition of pcr amplification reaction: 94 DEG C of initial denaturation 5min；94 DEG C of denaturation 30s；60 DEG C are moved back
Fiery 30s；72 DEG C of extension 5min；35 circulations.
Preferred technical solution are as follows: 6ulPCR is amplified into the product come and is mixed with 2ul loading buffer, is used in combination
Volume fraction is that 1-2% Ago-Gel carries out electrophoresis, is then clapped with EC3 Imaging System gel imaging system
According to；The Gene Finder fluorescent dye of 5ul is added in the Ago-Gel.
Since above-mentioned technical proposal is used, the present invention has the advantage, that compared with prior art
Method of the invention is used to identify the mating type of spot jade gill fungus White strain monocaryon bacterial strain, has time-consuming short, easy to operate,
Precision is high, advantage at low cost.
Detailed description of the invention
Fig. 1 is the electrophoretogram that primer HM1F/1R distinguishes spot jade gill fungus white monocaryon AxBx and AyBy mating type.
Fig. 2 is the electrophoretogram that primer HM2F/2R distinguishes spot jade gill fungus white monocaryon AxBx and AyBy mating type.
Fig. 3 is the electrophoretogram that primer HM3F/3R distinguishes spot jade gill fungus white monocaryon AxBx and AyBy mating type.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily.
Please refer to Fig. 1-3.It should be clear that this specification structure depicted in this specification institute accompanying drawings, ratio, size etc., only to match
The revealed content of specification is closed, so that those skilled in the art understands and reads, is not intended to limit the invention implementable
Qualifications, therefore do not have technical essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size,
In the case where not influencing the effect of present invention can be generated and the purpose that can reach, should all still fall in disclosed technology
In the range of Rong get Neng is covered.Meanwhile it is cited such as "upper", "lower", "left", "right", " centre " and " one " in this specification
Deng term, be merely convenient to being illustrated for narration, rather than to limit the scope of the invention, the change of relativeness
Or adjustment, under the content of no substantial changes in technology, when being also considered as the enforceable scope of the present invention.
Embodiment 1: a kind of method and primer of mating type that identifying spot jade gill fungus White strain monocaryon
A kind of method and primer for identifying spot jade gill fungus White strain monocaryon, the nucleotide sequence of primer pair are as follows:
HM1F: 5'- ACCTCTACTTCATCCTTGC-3'；
HM1R: 5'- TCTTGGGCGTCCGTTT-3'；
The special primer is exactly the sequence design according to AxBx mating type.Design principle is exactly the principle of PCR, base pairing and one
It causes.Band can be amplified with specific primer PCR, illustrate that its sequence and the sequence that cannot be expanded there is difference.
The method of identification spot jade gill fungus White strain monocaryon includes: to extract the genomic DNA of spot jade gill fungus white mononuclear bacterial strain,
Then PCR amplification, the production that PCR amplification comes out are carried out with first group of primer pair, second group of primer pair and third group primer pair respectively
Object is detected with 1% agarose gel electrophoresis；As shown in Figure 1.If cannot be considered as with primer amplified shaping band
AxBx mating type bacterial strain, if can be considered as AyBy mating type bacterial strain with primer amplified shaping band.
The method for extracting the genomic DNA of spot jade gill fungus White strain monocaryon includes the following steps: a, by spot jade gill fungus white
Bacterial strain monocaryon is cultivated in PDA plate culture medium；B, picking mycelia is placed in DNA lysate after culture, at 75 DEG C
After warm bath 5-10min to obtain the final product.The preparation of spot jade gill fungus White strain monocaryon: from leukasmus jade gill fungus Strain Protoplast regenerated plate
Middle separation obtains 343 plants of single Protoplast regeneration strains, observes 102 plants of mycelia without clamp connection under inverted fluorescence microscope
Leukasmus jade gill fungus bacterial strain monocaryon, randomly choose 21 plants of monocaryons and tested.
The system of pcr amplification reaction are as follows: 150ng/ul spot jade gill fungus White strain monocaryon DNA2ul, 10umol/L primer
2 ul of 2ul, 0.1mmol/LdNTP, high fidelity enzyme Pfu0.5ul, 5ul loading buffer, uses ddH2O polishing 25ul.
The condition of pcr amplification reaction: 94 DEG C of initial denaturation 5min；94 DEG C of denaturation 30s；60 DEG C of annealing 30s；72 DEG C of extensions
5min；35 circulations.
6ulPCR is amplified the product come to mix with 2ul loading buffer, and is 1% agarose with volume fraction
Gel carries out electrophoresis, is then taken pictures with EC3 Imaging System gel imaging system；Add in the Ago-Gel
Added with the Gene Finder fluorescent dye of 5ul.
Embodiment 2: a kind of method and primer of mating type that identifying spot jade gill fungus White strain monocaryon
A kind of method and primer for identifying spot jade gill fungus White strain monocaryon, the nucleotide sequence of primer pair are as follows:
HM2F: 5'-CGTCCCAGATATTCAACTC-3'；
HM2R: 5'-GAAACCTCCAACGCACT-3'；
The special primer is exactly the sequence design according to AxBx mating type.Design principle is exactly the principle of PCR, base pairing and one
It causes.Band can be amplified with specific primer PCR, illustrate that its sequence and the sequence that cannot be expanded there is difference.
The method of identification spot jade gill fungus White strain monocaryon includes: to extract the genomic DNA of spot jade gill fungus white mononuclear bacterial strain,
Then PCR amplification, the production that PCR amplification comes out are carried out with first group of primer pair, second group of primer pair and third group primer pair respectively
Object is detected with 1.5% agarose gel electrophoresis, as shown in Figure 2；If cannot be considered with primer amplified shaping band
It is AxBx mating type bacterial strain, if can be considered as AyBy mating type bacterial strain with primer amplified shaping band.
Preferred embodiment are as follows: the method for extracting the genomic DNA of spot jade gill fungus White strain monocaryon includes following step
It is rapid: a, to cultivate spot jade gill fungus White strain monocaryon in PDA plate culture medium；B, picking mycelia is placed on after culture
In DNA lysate, after 80 DEG C of warm bath 5-10min to obtain the final product.The preparation of spot jade gill fungus White strain monocaryon: from leukasmus jade gill fungus bacterium
Separation obtains 343 plants of single Protoplast regeneration strains in strain protoplast regeneration plate, observes under inverted fluorescence microscope
Leukasmus jade gill fungus bacterial strain monocaryon of 102 plants of mycelia without clamp connection, randomly chooses 21 plants of monocaryons and is tested, same to embodiment
One.
Preferred embodiment are as follows: the system of pcr amplification reaction are as follows: 150ng/ul spot jade gill fungus White strain monocaryon
DNA2ul, 10umol/L primer 2 ul, 0.1mmol/LdNTP 2 ul, high fidelity enzyme Pfu0.5ul, 5ul loading
Buffer uses ddH2O polishing 25ul.
Preferred embodiment are as follows: the condition of pcr amplification reaction: 94 DEG C of initial denaturation 5min；94 DEG C of denaturation 30s；60 DEG C are moved back
Fiery 30s；72 DEG C of extension 5min；35 circulations.
Preferred technical solution are as follows: 6ulPCR is amplified into the product come and is mixed with 2ul loading buffer, is used in combination
Volume fraction is that 2% Ago-Gel carries out electrophoresis, is then taken pictures with EC3 Imaging System gel imaging system；
The Gene Finder fluorescent dye of 5ul is added in the Ago-Gel.
Embodiment 3: a kind of method and primer of mating type that identifying spot jade gill fungus White strain monocaryon
A kind of method and primer for identifying spot jade gill fungus White strain monocaryon, the nucleotide sequence of primer pair are as follows:
HM3F: 5'-CTGGCGTTACCTTCCC-3'；
HM3R: 5'-GTGGTTGTGGCGTGAT-3'；
The special primer is exactly the sequence design according to AxBx mating type.Design principle is exactly the principle of PCR, base pairing and one
It causes.Band can be amplified with specific primer PCR, illustrate that its sequence and the sequence that cannot be expanded there is difference.
The method of identification spot jade gill fungus White strain monocaryon includes: to extract the genomic DNA of spot jade gill fungus white mononuclear bacterial strain,
Then PCR amplification, the production that PCR amplification comes out are carried out with first group of primer pair, second group of primer pair and third group primer pair respectively
Object is detected with the agarose gel electrophoresis of 1%-2%, as shown in Figure 3；If cannot being recognized with primer amplified shaping band
To be AxBx mating type bacterial strain, if can be considered as AyBy mating type bacterial strain with primer amplified shaping band.
Preferred embodiment are as follows: the method for extracting the genomic DNA of spot jade gill fungus White strain monocaryon includes following step
It is rapid: a, to cultivate spot jade gill fungus White strain monocaryon in PDA plate culture medium；B, picking mycelia is placed on after culture
In DNA lysate, after 85 DEG C of warm bath 5-10min to obtain the final product.The preparation of spot jade gill fungus White strain monocaryon: from leukasmus jade gill fungus bacterium
Separation obtains 343 plants of single Protoplast regeneration strains in strain protoplast regeneration plate, observes under inverted fluorescence microscope
Leukasmus jade gill fungus bacterial strain monocaryon of 102 plants of mycelia without clamp connection, randomly chooses 21 plants of monocaryons and is tested, same to embodiment
One.
Preferred embodiment are as follows: the system of pcr amplification reaction are as follows: 150ng/ul spot jade gill fungus White strain monocaryon
DNA2ul, 10umol/L primer 2 ul, 0.1mmol/LdNTP 2 ul, high fidelity enzyme Pfu0.5ul, 5ul loading
Buffer uses ddH2O polishing 25ul.
Preferred embodiment are as follows: the condition of pcr amplification reaction: 94 DEG C of initial denaturation 5min；94 DEG C of denaturation 30s；60 DEG C are moved back
Fiery 30s；72 DEG C of extension 5min；35 circulations.
Preferred embodiment are as follows: 6ulPCR is amplified into the product come and is mixed with 2ul loading buffer, is used in combination
Volume fraction is that 1-2% Ago-Gel carries out electrophoresis, is then clapped with EC3 Imaging System gel imaging system
According to；The Gene Finder fluorescent dye of 5ul is added in the Ago-Gel.
As described above is only to be not intended to tool to explain the preferred embodiments of the invention to do any shape to the present invention
Limitation in formula should all wrap therefore all have any modification or change for making the related present invention under identical spirit
It includes in the scope that the invention is intended to protect.
Sequence table
<110>Academy of Agricultural Sciences, Shanghai City
<120>a kind of method and primer of the mating type for identifying spot jade gill fungus White strain monocaryon
<130> 20190729
<160> 6
<210> 1
<211> 19
<212> DNA
<213>artificial sequence
<223> HM1F
<400> 1 ACCTCTACTT CATCCTTGC 19
<210> 2
<211> 16
<212> DNA
<213>artificial sequence
<223> HM1R
<400> 2 TCTTGGGCGT CCGTTT 16
<210> 3
<211> 19
<212> DNA
<213>artificial sequence
<223> HM2F
<400> 3 CGTCCCAGAT ATTCAACTC 19
<210> 4
<211> 16
<212> DNA
<213>artificial sequence
<223> HM2R
<400> 4 GAAACCTCCA ACGCACT 17
<210> 5
<211> 19
<212> DNA
<213>artificial sequence
<223> HM3F
<400> 5 CTGGCGTTAC CTTCCC 16
<210> 6
<211> 16
<212> DNA
<213>artificial sequence
<223> HM3R
<400> 6 GTGGTTGTGG CGTGAT 16
Claims (6)
1. a kind of primer for identifying spot jade gill fungus White strain monocaryon, it is characterised in that: including 3 groups of primer pairs, first group of primer
Pair nucleotide sequence it is as follows:
HM1F: 5'- ACCTCTACTTCATCCTTGC-3'；
HM1R: 5'- TCTTGGGCGTCCGTTT-3'；
The nucleotide sequence of second group of primer pair is as follows:
HM2F: 5'-CGTCCCAGATATTCAACTC-3'；
HM2R: 5'-GAAACCTCCAACGCACT-3'；
The nucleotide sequence of third group primer pair is as follows:
HM3F: 5'-CTGGCGTTACCTTCCC-3'；
HM3R: 5’-GTGGTTGTGGCGTGAT-3’。
2. a kind of method for identifying spot jade gill fungus White strain monocaryon, it is characterised in that: including 3 groups of primer pairs, first group of primer
Pair nucleotide sequence it is as follows:
HM1F: 5'- ACCTCTACTTCATCCTTGC-3'；
HM1R: 5'- TCTTGGGCGTCCGTTT-3'；
The nucleotide sequence of second group of primer pair is as follows:
HM2F: 5'-CGTCCCAGATATTCAACTC-3'；
HM2R: 5'-GAAACCTCCAACGCACT-3'；
The nucleotide sequence of third group primer pair is as follows:
HM3F: 5'-CTGGCGTTACCTTCCC-3'；
HM3R: 5'-GTGGTTGTGGCGTGAT-3'；
The method of identification spot jade gill fungus White strain monocaryon includes: to extract the genomic DNA of spot jade gill fungus white mononuclear bacterial strain, then
PCR amplification is carried out with first group of primer pair, second group of primer pair and third group primer pair respectively, the product that PCR amplification comes out is used
The agarose gel electrophoresis of 1%-2% detects；If cannot be considered as AxBx mating type with primer amplified shaping band
Bacterial strain, if can be considered as AyBy mating type bacterial strain with primer amplified shaping band.
3. the method for identification spot jade gill fungus White strain monocaryon according to claim 2, it is characterised in that: extract spot jade gill fungus
The method of the genomic DNA of White strain monocaryon includes the following steps: a, by spot jade gill fungus White strain monocaryon in PDA plate
Culture medium is cultivated；B, picking mycelia is placed in DNA lysate after culture, after 75-85 DEG C of warm bath 5-10min i.e.
?.
4. the method for identification spot jade gill fungus White strain monocaryon according to claim 2, it is characterised in that: PCR amplification is anti-
The system answered are as follows: the 10umol/L primer of 150ng/ul spot jade gill fungus White strain the monocaryon DNA, 2ul of 2ul, 2ul's
The loading buffer of the high fidelity enzyme Pfu, 5ul of 0.1mmol/LdNTP, 0.5ul, use ddH2O polishing 25ul.
5. the method for identification spot jade gill fungus White strain monocaryon according to claim 2, it is characterised in that: PCR amplification is anti-
The condition answered: 94 DEG C of initial denaturation 5min；94 DEG C of denaturation 30s；60 DEG C of annealing 30s；72 DEG C of extension 5min；35 circulations.
6. the method for identification spot jade gill fungus White strain monocaryon according to claim 2, it is characterised in that: expand 6ulPCR
Increase the product come out to mix with the loading buffer of 2ul, and be that 1-2% Ago-Gel carries out electrophoresis with volume fraction, so
It is taken pictures afterwards with EC3 Imaging System gel imaging system；The Gene of 5ul is added in the Ago-Gel
Finder fluorescent dye.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112126700A (en) * | 2020-09-16 | 2020-12-25 | 广东省科学院生物工程研究所 | Primer pair and kit for identifying mating type of ganoderma lucidum protoplast monokaryon and application of primer pair and kit |
| CN112126701A (en) * | 2020-09-16 | 2020-12-25 | 广东省科学院生物工程研究所 | Primer pair and kit for identifying mating types of protoplast monokaryons of Leptosphaeria scoparia and application of primer pair and kit |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000125659A (en) * | 1998-10-28 | 2000-05-09 | Katsuyama Technos:Kk | Culture of pseudo tricholoma matsutake |
| CN101608213A (en) * | 2008-06-17 | 2009-12-23 | 上海市农业科学院 | A kind of discrimination method of hypsizigus marmoreus mating type gene |
| CN103305606A (en) * | 2013-05-20 | 2013-09-18 | 北京市农林科学院 | Method for identifying mating type of protoplasted monokaryon of lepista sordida and special primer pair SR-6*4 thereof |
| CN108611435A (en) * | 2018-05-21 | 2018-10-02 | 上海市农业科学院 | A kind of identification agaricus bisporus is the same as Genetic Sterility single-ascospore strain and its method and primer of mating type |
-
2019
- 2019-07-29 CN CN201910689443.9A patent/CN110358858A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000125659A (en) * | 1998-10-28 | 2000-05-09 | Katsuyama Technos:Kk | Culture of pseudo tricholoma matsutake |
| CN101608213A (en) * | 2008-06-17 | 2009-12-23 | 上海市农业科学院 | A kind of discrimination method of hypsizigus marmoreus mating type gene |
| CN103305606A (en) * | 2013-05-20 | 2013-09-18 | 北京市农林科学院 | Method for identifying mating type of protoplasted monokaryon of lepista sordida and special primer pair SR-6*4 thereof |
| CN108611435A (en) * | 2018-05-21 | 2018-10-02 | 上海市农业科学院 | A kind of identification agaricus bisporus is the same as Genetic Sterility single-ascospore strain and its method and primer of mating type |
Non-Patent Citations (3)
| Title |
|---|
| CHANG Y. LEE ET AL.: ""Development of new strains and related SCAR markers for an edible mushroom, Hypsizygus marmoreus"", 《FEMS MICROBIOL LETT》 * |
| 许占伍: ""真姬菇颜色遗传及单核体多态性初步分析"", 《万方学位论文》 * |
| 陈志娥: ""斑玉蕈SCAR标记遗传连锁图的构建"", 《万方学位论文》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112126700A (en) * | 2020-09-16 | 2020-12-25 | 广东省科学院生物工程研究所 | Primer pair and kit for identifying mating type of ganoderma lucidum protoplast monokaryon and application of primer pair and kit |
| CN112126701A (en) * | 2020-09-16 | 2020-12-25 | 广东省科学院生物工程研究所 | Primer pair and kit for identifying mating types of protoplast monokaryons of Leptosphaeria scoparia and application of primer pair and kit |
| CN112126701B (en) * | 2020-09-16 | 2022-04-22 | 广东省科学院生物工程研究所 | Primer pair and kit for identifying mating types of protoplast monokaryons of Leptosphaeria scoparia and application of primer pair and kit |
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